Rroxscaffold_4G00324060

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001590
Physical Location & Seq
Forward (+)
55348020 .. 55348734
715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324060.1

Sequence Viewer

Length: 450 bp
ATGTTGCGTTCGGTTGCTCGCTTGGCCGGACACGTGGATGAGGCAAGTGCAAGTGATGAAGCTAAGGTTGTAGAAGAACAAGATAAGAAATTGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAAATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGATTGAAAACTCGCCCTGGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCGGGGAGTACTAGTCCGGTAGATGGGTATCCATGGAGTTCTGTGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCGGGGTGAATGATGTTTGTCAAGCGACTAGTTCTCGTGGTTGTCCCAAAAAGTCCGAAGGCGCATTTGGAAGAATTGTGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

149

Amino Acids

15.84

Weight (kDa)

6.13

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 117
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 1 cut(s) 281
AflIII ACRYGT 2 cut(s) 31, 317
AgsI TTSAA 3 cut(s) 181, 232, 243
AhlI ACTAGT 2 cut(s) 269, 389
AjnI CCWGG 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 411, 443
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 149, 152
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 379
BauI CACGAG 1 cut(s) 396
BbrPI CACGTG 1 cut(s) 34
BbvI GCAGC 2 cut(s) 136, 139
BccI CCATC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 192
BciVI GTATCC 1 cut(s) 297
BcnI CCSGG 1 cut(s) 364
BcuI ACTAGT 2 cut(s) 269, 389
BfaI CTAG 2 cut(s) 270, 390
BfuAI ACCTGC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 297
BisI GCNGC 2 cut(s) 150, 153
BlsI GCNGC 2 cut(s) 151, 154
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 2 cut(s) 192, 364
BmrFI CCNGG 2 cut(s) 192, 364
Bpu10I CCTNAGC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 190, 290, 363
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 190, 290, 363
BseGI GGATG 1 cut(s) 43
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 281
BseXI GCAGC 2 cut(s) 136, 139
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 1 cut(s) 26
BsiSI CCGG 3 cut(s) 27, 275, 363
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 1 cut(s) 26
Bsp19I CCATGG 1 cut(s) 290
BspANI GGCC 1 cut(s) 26
BspMI ACCTGC 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 190, 290, 363
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 192
Bst6I CTCTTC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 1 cut(s) 43
BstHHI GCGC 1 cut(s) 425
BstMWI GCNNNNNNNGC 2 cut(s) 23, 161
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 190, 362
BstV1I GCAGC 2 cut(s) 136, 139
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 1 cut(s) 43
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 267
CviAII CATG 3 cut(s) 291, 313, 318
CviJI RGCY 4 cut(s) 26, 62, 95, 236
CviKI_1 RGCY 4 cut(s) 26, 62, 95, 236
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 117
Eco72I CACGTG 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 290
EcoT22I ATGCAT 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 3 cut(s) 294, 316, 321
FaiI YATR 8 cut(s) 128, 173, 218, 248, 292, 314, 319, 351
FaqI GGGAC 1 cut(s) 390
FatI CATG 3 cut(s) 290, 312, 317
Fnu4HI GCNGC 2 cut(s) 150, 153
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 2 cut(s) 150, 153
FspBI CTAG 2 cut(s) 270, 390
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 150, 153
HaeIII GGCC 1 cut(s) 26
HapII CCGG 3 cut(s) 27, 275, 363
HhaI GCGC 1 cut(s) 425
Hin1II CATG 3 cut(s) 294, 316, 321
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HinfI GANTC 1 cut(s) 239
HpaII CCGG 3 cut(s) 27, 275, 363
HphI GGTGA 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 109, 418
HpyAV CCTTC 2 cut(s) 189, 413
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 5 cut(s) 50, 155, 304, 312, 336
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 161
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 3 cut(s) 294, 316, 321
HspAI GCGC 1 cut(s) 423
LpnPI CCDG 6 cut(s) 40, 177, 204, 288, 316, 376
Lsp1109I GCAGC 2 cut(s) 136, 139
MaeI CTAG 2 cut(s) 270, 390
MaeII ACGT 1 cut(s) 33
MboII GAAGA 3 cut(s) 86, 110, 444
MluCI AATT 3 cut(s) 89, 139, 435
MnlI CCTC 3 cut(s) 34, 94, 346
Mph1103I ATGCAT 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 3 cut(s) 27, 275, 363
MspR9I CCNGG 2 cut(s) 192, 364
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 23, 161
NciI CCSGG 1 cut(s) 364
NcoI CCATGG 1 cut(s) 290
NlaIII CATG 3 cut(s) 294, 316, 321
NsiI ATGCAT 1 cut(s) 314
NspI RCATGY 1 cut(s) 321
PaqCI CACCTGC 1 cut(s) 321
PciI ACATGT 1 cut(s) 317
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 151, 154
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 34
PscI ACATGT 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 190
PspCI CACGTG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 190
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 1 cut(s) 36
SatI GCNGC 2 cut(s) 150, 153
ScaI AGTACT 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 192, 364
SetI ASST 5 cut(s) 36, 64, 69, 105, 335
SmiMI CAYNNNNRTG 1 cut(s) 36
SpeI ACTAGT 2 cut(s) 269, 389
Sse9I AATT 3 cut(s) 89, 139, 435
SspMI CTAG 2 cut(s) 270, 390
StyD4I CCNGG 2 cut(s) 190, 362
StyI CCWWGG 1 cut(s) 290
TaiI ACGT 1 cut(s) 36
TasI AATT 3 cut(s) 89, 139, 435
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 1 cut(s) 239
TseI GCWGC 2 cut(s) 149, 152
TspDTI ATGAA 2 cut(s) 72, 216
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 321
XspI CTAG 2 cut(s) 270, 390
ZrmI AGTACT 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.