Rorug01G0131300

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001590
Physical Location & Seq
Reverse (-)
22265880 .. 22270199
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0131300.1

Sequence Viewer

Length: 2142 bp
ATGTATCAGTACAATTCCTCAGGAATGGACAGTGCAGGAAAAATGGAACAGGCTGCATATGTAGACTATATAAAATCAAAGAAAATGCTTGCGAGCTGTGAGACCCACAAGAAAGAACTCATGAAGGCGCTTGAACAGAGTGAAGTTGAAAAGGAAGACCTGAAGAGGCAGATTGAGAAAAGCAAAACAGAAAAAGAAGAGCTTAAGAAAGATCTTGAGTGGAGCGAGGCTCTGAAGCATGATCTCAAGAAAGAGCTTGTGAAGAGTGAATCTCATAAGAAAGAACTCAAGAATAAAGTGTCTGAGTTGCAGGGTGAGGCTGAGAAATATTTTACGAAGAAAATAGAGTTTATGAGGATTAATCTTCAACAACATAGTAGAGATCTTGAGGAAAAACTCAATATGAAGGAATCTGAGACAGTTGAGCTTAAGAAACAGCTCAAGAAGATTGAGGCTCAAAAGGAAGATCTTAAGTATGAGCTTGAGCAGACGACAGTTGAGAAAGAATATCTTGAAGCTGAAAAGGAAAAACTTGCAACTGAAAATGAAGAGTTTGAAACTGAAATGATATATCTTGAGGAAGAGTTTGCGCAGAGTGAAGCTCAAAAGGAAAACCTTAAGAAACAACTTGAGAAGAGTGAAGCTCATAAAAAAGAGCTAAAGGAAGCTCTTGAGGAGAGCGAAGCGCAAAAGAATGATCTTGTGGAGAAGCTTAGTTTGAGTCAAGCTATAAACAAAGATCTTAAAAAAAAACTTGAGCATTGTGAAGCTAAAAAAAAGGAACTTGAAAATGAAAATGAACGGCTTCAGCAAGAGCTTGAGGAGAGCAAAGCTGAAAAGATCGAGATTCAAAAAGAACTTGAGCAACTTGATATGCAACTGCAACAAGTGCAAAGTGAAGCTCAAAAGGAAGACCTTAAGAAAGAGCTCGAGCAATCTGAAGTTGAGAAGGAAAATCTAAAGAAAAAACTTGAGAAGAGTGATGCTCAAAATGAAAATTTAAAGAAAAGGATTGAGCAGATTGAAGCTGAAAAGAAACATATTGAGGAAAAGCTTGAGGAGTGTGAAACTGAAAGAGACGATATTGAAGCTGAAAAAGAAGATCTTGAGGAAAGGCTCGAGGAGTGTGAAGCGGAAAAGGAAGATCTTGAAGGAGAAAAGGAAGATCTTGAGGAAAAGCTTGAGCAAAGCCTCGCTAAAAAGGCTCATCTTAAGAAAGAGCTTGAGCAAACTGAAGCTCTAAATGAAGATCTTAAGAAAAAAATTGATCAGAGTGAAGTTGTAAGAGCAGATCTTAAGAAAGAGCTTGAGCAGAGTGATGCTGAAAAGATAGAACTAAAGAAAGAACTTGACCAACGGGAGTTGAAACTAGAAAAAATGCAAATTAGTGAAGCTGAAAAGGAAGAATATTTTAAGAATGAGCTAGCACAAAATGAACTGGAAAAAGAAGATATTAAGCAGGAACTTGAGAATAGCGTAGCTATAAATAAAGATCTTCAGAAAACACTTGAGGATAGTGATGCTGAAAAGGAGGAACTTAAGGAAAAGCTCAAGAAGAGGGAAACTGAAAAGGAAGAGCTTAAGGAAGTTGTTGTGGAGATAGAAGCTAAAAAGGTGGATCTTAAGAGGGAGCTTGAGCGGACTGCAGCTGAAAAGAAAGAGCTTACCAAAGTACTAGAGGAGAAAAAACTGAAACGAATGCAGAGTGAAGTTGAAATGGAAGCATTTAAAAAGCAGATTGAAGTTGAAGCGGAAGATGTTAAGAGAGAACTTGAGATGAGCAAAGCTGAAATGATCACATTTAAAAAGCAGAGTGAAGTTGAAATGCTAGCTGTTGAGAAAGAACTTGAGGTGAGCAAAGCTGAAAAGATGGCATTTAAAAAATTACTTCAGCAGCGTGATCTAGAACTTCAACAACTGGTGGAACACACGGATAAGAAAGTTACTTTAGAGCAAAACAAAGTTGATGACTTGCTGGCAGACTTGGCAAAGGAACGCAAGCAATGGCAGAGTGAAAAGGAAAGACTTCAATGGATCAATGCTGAATTGGAAAAGCAGCTTGAAGATGTCAACAAGAAAAACATCAGCATAGAGGAAGATGATGAGTTAGAAGTTGTTGATGATGCGTGGTTGAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

713

Amino Acids

84.71

Weight (kDa)

4.87

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 591
AccBSI CCGCTC 1 cut(s) 1639
AccI GTMKAC 1 cut(s) 63
AciI CCGC 3 cut(s) 1133, 1639, 1751
AclWI GGATC 2 cut(s) 1626, 2042
AcsI RAATTY 2 cut(s) 997, 2136
AcuI CTGAAG 7 cut(s) 182, 254, 791, 960, 1254, 1481, 1874
AfaI GTAC 2 cut(s) 11, 1674
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 3 cut(s) 95, 410, 1071
AlwI GGATC 2 cut(s) 1626, 2042
Ama87I CYCGRG 2 cut(s) 929, 1118
ApeKI GCWGC 4 cut(s) 53, 1646, 1894, 2056
ApoI RAATTY 2 cut(s) 997, 2136
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 2 cut(s) 804, 2025
AspLEI GCGC 3 cut(s) 130, 592, 688
AsuHPI GGTGA 2 cut(s) 326, 1864
AsuNHI GCTAGC 2 cut(s) 1423, 1828
AvaI CYCGRG 2 cut(s) 929, 1118
AxyI CCTNAGG 1 cut(s) 19
BanII GRGCYC 1 cut(s) 930
BbsI GAAGAC 2 cut(s) 162, 918
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 4 cut(s) 40, 1658, 1906, 2068
BccI CCATC 1 cut(s) 1864
BceAI ACGGC 1 cut(s) 818
BclI TGATCA 2 cut(s) 1267, 1794
BcoDI GTCTC 3 cut(s) 95, 410, 1071
BfaI CTAG 5 cut(s) 1370, 1424, 1676, 1829, 1904
BfmI CTRYAG 1 cut(s) 1644
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 4 cut(s) 54, 1647, 1895, 2057
BlsI GCNGC 4 cut(s) 55, 1648, 1896, 2058
BmcAI AGTACT 1 cut(s) 1674
BmeT110I CYCGRG 2 cut(s) 929, 1118
BmsI GCATC 4 cut(s) 973, 1309, 1510, 2113
BmtI GCTAGC 2 cut(s) 1427, 1832
BpiI GAAGAC 2 cut(s) 162, 918
BsaI GGTCTC 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 1443, 1923
Bse21I CCTNAGG 1 cut(s) 19
Bse3DI GCAATG 1 cut(s) 2009
BseMI GCAATG 1 cut(s) 2009
BseMII CTCAG 4 cut(s) 33, 294, 312, 405
BseNI ACTGG 2 cut(s) 1443, 1923
BseRI GAGGAG 5 cut(s) 689, 836, 1073, 1136, 1694
BseXI GCAGC 4 cut(s) 40, 1658, 1906, 2068
BsgI GTGCAG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 2 cut(s) 929, 1118
BsmAI GTCTC 3 cut(s) 95, 410, 1071
BsmBI CGTCTC 1 cut(s) 1071
BsmI GAATGC 1 cut(s) 1704
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 929, 1118
Bsp1286I GDGCHC 1 cut(s) 930
BspACI CCGC 3 cut(s) 1133, 1639, 1751
BspCNI CTCAG 4 cut(s) 32, 295, 313, 406
BspHI TCATGA 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 1648
BspOI GCTAGC 2 cut(s) 1427, 1832
BspPI GGATC 2 cut(s) 1626, 2042
BspQI GCTCTTC 2 cut(s) 192, 1569
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 1639
BsrDI GCAATG 1 cut(s) 2009
BsrI ACTGG 2 cut(s) 1443, 1923
Bst4CI ACNGT 3 cut(s) 32, 421, 496
Bst6I CTCTTC 9 cut(s) 158, 192, 257, 543, 576, 629, 971, 1550, 1569
BstAPI GCANNNNNTGC 1 cut(s) 889
BstC8I GCNNGC 6 cut(s) 90, 94, 1425, 1830, 1977, 2000
BstDEI CTNAG 5 cut(s) 19, 303, 321, 414, 713
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 3 cut(s) 130, 592, 688
BstMAI GTCTC 3 cut(s) 95, 410, 1071
BstMWI GCNNNNNNNGC 3 cut(s) 889, 1202, 1985
BstSFI CTRYAG 1 cut(s) 1644
BstV1I GCAGC 4 cut(s) 40, 1658, 1906, 2068
BstV2I GAAGAC 2 cut(s) 162, 918
Bsu36I CCTNAGG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 37
Cac8I GCNNGC 6 cut(s) 90, 94, 1425, 1830, 1977, 2000
CciI TCATGA 1 cut(s) 120
CfoI GCGC 3 cut(s) 130, 592, 688
Csp6I GTAC 2 cut(s) 10, 1673
CviAII CATG 2 cut(s) 121, 239
CviQI GTAC 2 cut(s) 10, 1673
DdeI CTNAG 5 cut(s) 19, 303, 321, 414, 713
DraI TTTAAA 4 cut(s) 1002, 1729, 1804, 1879
Eam1104I CTCTTC 9 cut(s) 158, 192, 257, 543, 576, 629, 971, 1550, 1569
EarI CTCTTC 9 cut(s) 158, 192, 257, 543, 576, 629, 971, 1550, 1569
Ecl136II GAGCTC 1 cut(s) 928
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 7 cut(s) 182, 254, 791, 960, 1254, 1481, 1874
Eco81I CCTNAGG 1 cut(s) 19
Eco88I CYCGRG 2 cut(s) 929, 1118
EcoICRI GAGCTC 1 cut(s) 928
EcoT38I GRGCYC 1 cut(s) 930
Esp3I CGTCTC 1 cut(s) 1071
FaeI CATG 2 cut(s) 124, 242
FalI AAGNNNNNCTT 6 cut(s) 186, 218, 495, 527, 1089, 1121
FatI CATG 2 cut(s) 120, 238
FauNDI CATATG 1 cut(s) 58
FbaI TGATCA 2 cut(s) 1267, 1794
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 54, 1647, 1895, 2057
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 4 cut(s) 54, 1647, 1895, 2057
FspBI CTAG 5 cut(s) 1370, 1424, 1676, 1829, 1904
FspI TGCGCA 1 cut(s) 591
GlaI GCGC 3 cut(s) 129, 591, 687
GluI GCNGC 4 cut(s) 54, 1647, 1895, 2057
HaeII RGCGCY 1 cut(s) 131
HhaI GCGC 3 cut(s) 130, 592, 688
Hin1II CATG 2 cut(s) 124, 242
Hin6I GCGC 3 cut(s) 128, 590, 686
HinP1I GCGC 3 cut(s) 128, 590, 686
HincII GTYRAC 1 cut(s) 2071
HindII GTYRAC 1 cut(s) 2071
HindIII AAGCTT 3 cut(s) 710, 1052, 1178
HinfI GANTC 4 cut(s) 269, 410, 721, 847
HphI GGTGA 2 cut(s) 326, 1864
Hpy166II GTNNAC 2 cut(s) 64, 2071
Hpy188I TCNGA 6 cut(s) 234, 304, 415, 940, 1272, 1500
Hpy8I GTNNAC 2 cut(s) 64, 2071
HpyAV CCTTC 4 cut(s) 118, 400, 943, 1145
HpyCH4III ACNGT 3 cut(s) 32, 421, 496
HpyF10VI GCNNNNNNNGC 3 cut(s) 889, 1202, 1985
HpyF3I CTNAG 5 cut(s) 19, 303, 321, 414, 713
Hsp92II CATG 2 cut(s) 124, 242
HspAI GCGC 3 cut(s) 128, 590, 686
Ksp22I TGATCA 2 cut(s) 1267, 1794
LguI GCTCTTC 2 cut(s) 192, 1569
LmnI GCTCC 2 cut(s) 222, 1630
LpnPI CCDG 9 cut(s) 6, 21, 35, 173, 296, 1424, 1445, 1904, 1961
Lsp1109I GCAGC 4 cut(s) 40, 1658, 1906, 2068
LweI GCATC 4 cut(s) 973, 1309, 1510, 2113
MaeI CTAG 5 cut(s) 1370, 1424, 1676, 1829, 1904
MaeIII GTNAC 1 cut(s) 1942
MbiI CCGCTC 1 cut(s) 1639
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 7 cut(s) 13, 997, 1263, 1383, 1883, 2045, 2136
MlyI GAGTC 1 cut(s) 730
MroXI GAANNNNTTC 2 cut(s) 804, 2025
MspA1I CMGCKG 1 cut(s) 1649
Mva1269I GAATGC 1 cut(s) 1704
MwoI GCNNNNNNNGC 3 cut(s) 889, 1202, 1985
NdeI CATATG 1 cut(s) 58
NheI GCTAGC 2 cut(s) 1423, 1828
NlaIII CATG 2 cut(s) 124, 242
NsbI TGCGCA 1 cut(s) 591
PaeR7I CTCGAG 2 cut(s) 929, 1118
PagI TCATGA 1 cut(s) 120
PciSI GCTCTTC 2 cut(s) 192, 1569
PctI GAATGC 1 cut(s) 1704
PdmI GAANNNNTTC 2 cut(s) 804, 2025
PfeI GAWTC 3 cut(s) 269, 410, 847
PkrI GCNGC 4 cut(s) 55, 1648, 1896, 2058
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
PshBI ATTAAT 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 930
PspXI VCTCGAGB 2 cut(s) 929, 1118
PstI CTGCAG 1 cut(s) 1648
PvuII CAGCTG 1 cut(s) 1649
RsaI GTAC 2 cut(s) 11, 1674
RsaNI GTAC 2 cut(s) 10, 1673
SacI GAGCTC 1 cut(s) 930
SapI GCTCTTC 2 cut(s) 192, 1569
SatI GCNGC 4 cut(s) 54, 1647, 1895, 2057
ScaI AGTACT 1 cut(s) 1674
SchI GAGTC 1 cut(s) 730
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 4 cut(s) 973, 1309, 1510, 2113
SfcI CTRYAG 1 cut(s) 1644
Sfr274I CTCGAG 2 cut(s) 929, 1118
SlaI CTCGAG 2 cut(s) 929, 1118
Sse9I AATT 7 cut(s) 13, 997, 1263, 1383, 1883, 2045, 2136
SsiI CCGC 3 cut(s) 1133, 1639, 1751
SspI AATATT 2 cut(s) 329, 1409
SspMI CTAG 5 cut(s) 1370, 1424, 1676, 1829, 1904
SstI GAGCTC 1 cut(s) 930
TaaI ACNGT 3 cut(s) 32, 421, 496
TaqI TCGA 3 cut(s) 843, 930, 1119
TasI AATT 7 cut(s) 13, 997, 1263, 1383, 1883, 2045, 2136
TatI WGTACW 2 cut(s) 9, 1672
TfiI GAWTC 3 cut(s) 269, 410, 847
TscAI CASTG 1 cut(s) 37
TseI GCWGC 4 cut(s) 53, 1646, 1894, 2056
TspDTI ATGAA 8 cut(s) 137, 419, 561, 807, 813, 1008, 1260, 1449
TspGWI ACGGA 1 cut(s) 1946
TspRI CASTG 1 cut(s) 37
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 2 cut(s) 997, 2136
XbaI TCTAGA 1 cut(s) 1903
XhoI CTCGAG 2 cut(s) 929, 1118
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 2 cut(s) 804, 2025
XspI CTAG 5 cut(s) 1370, 1424, 1676, 1829, 1904
ZrmI AGTACT 1 cut(s) 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.