Rroxscaffold_4G00314960

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001590
Physical Location & Seq
Forward (+)
39181061 .. 39182472
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00314960.1

Sequence Viewer

Length: 330 bp
ATGGTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTCTCTACAGGAACAATGAGGCGGTACAAGGTCTTCTCCTCCAGCACTAATGTCTCGTTACCATTCACAGCTCCAGACTCTCCGGCTGGGGTGGAATTATGCGCAGTCTCAGGTCGAAATACCCCATTTCGAGGGTTTGGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

109

Amino Acids

11.73

Weight (kDa)

5.69

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ACSF4 PF13570 24 - 57 1.2e-11 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 286
AciI CCGC 1 cut(s) 205
AcsI RAATTY 3 cut(s) 52, 68, 129
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 1 cut(s) 58
AjnI CCWGG 2 cut(s) 88, 180
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 3 cut(s) 190, 241, 295
Alw44I GTGCAC 1 cut(s) 158
ApaLI GTGCAC 1 cut(s) 158
ApoI RAATTY 3 cut(s) 52, 68, 129
AspLEI GCGC 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 162
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 162
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 2 cut(s) 90, 182
BcoDI GTCTC 3 cut(s) 190, 241, 295
BfaI CTAG 1 cut(s) 328
BfmI CTRYAG 1 cut(s) 189
Bme1390I CCNGG 2 cut(s) 90, 182
BmrFI CCNGG 2 cut(s) 90, 182
BmsI GCATC 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 2 cut(s) 208, 240
BsaI GGTCTC 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 88, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 90, 182
BseDI CCNNGG 2 cut(s) 88, 180
BseGI GGATG 2 cut(s) 31, 154
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 162
BseYI CCCAGC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 3 cut(s) 190, 241, 295
Bso31I GGTCTC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 162
BspACI CCGC 1 cut(s) 205
BspCNI CTCAG 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 88, 180
Bst2UI CCWGG 2 cut(s) 90, 182
Bst6I CTCTTC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 292
BstF5I GGATG 2 cut(s) 31, 154
BstHHI GCGC 1 cut(s) 287
BstMAI GTCTC 3 cut(s) 190, 241, 295
BstNI CCWGG 2 cut(s) 90, 182
BstSCI CCNGG 2 cut(s) 88, 180
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 208
BtsCI GGATG 2 cut(s) 31, 154
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 160
CfoI GCGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 208
CviJI RGCY 3 cut(s) 178, 254, 269
CviKI_1 RGCY 3 cut(s) 178, 254, 269
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 88, 180
FaiI YATR 2 cut(s) 156, 283
FokI GGATG 2 cut(s) 38, 161
FspBI CTAG 1 cut(s) 328
FspI TGCGCA 1 cut(s) 286
GlaI GCGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 2 cut(s) 208, 240
HapII CCGG 1 cut(s) 266
HhaI GCGC 1 cut(s) 287
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 3 cut(s) 10, 260, 324
HpaII CCGG 1 cut(s) 266
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 29
HpyCH4V TGCA 4 cut(s) 41, 74, 160, 165
HpyF3I CTNAG 1 cut(s) 292
HspAI GCGC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 259
LweI GCATC 1 cut(s) 16
MaeI CTAG 1 cut(s) 328
MaeIII GTNAC 1 cut(s) 240
MboII GAAGA 2 cut(s) 96, 208
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 5 cut(s) 19, 52, 68, 129, 278
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 4 cut(s) 58, 195, 232, 308
MseI TTAA 2 cut(s) 18, 174
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 90, 182
MvaI CCWGG 2 cut(s) 90, 182
NsbI TGCGCA 1 cut(s) 286
PfeI GAWTC 1 cut(s) 10
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 88, 180
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 2 cut(s) 88, 180
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 18, 174
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 90, 182
SduI GDGCHC 1 cut(s) 162
SetI ASST 7 cut(s) 18, 40, 80, 186, 216, 256, 298
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 189
Sse9I AATT 5 cut(s) 19, 52, 68, 129, 278
SsiI CCGC 1 cut(s) 205
SspMI CTAG 1 cut(s) 328
StyD4I CCNGG 2 cut(s) 88, 180
TaqI TCGA 2 cut(s) 298, 313
TasI AATT 5 cut(s) 19, 52, 68, 129, 278
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 2 cut(s) 18, 174
Tru9I TTAA 2 cut(s) 18, 174
TscAI CASTG 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 65
TspRI CASTG 1 cut(s) 167
VneI GTGCAC 1 cut(s) 158
XapI RAATTY 3 cut(s) 52, 68, 129
XspI CTAG 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.