Rw1G012430

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001590
Physical Location & Seq
Reverse (-)
27656181 .. 27666856
10676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012430.1

Sequence Viewer

Length: 3375 bp
ATGAGAAGCCATGAAGGTTCTATACTCCACCACTTCTCCAAAGCAGCGTCCCAAAACCTCAACAAAATCGCAGTCATACACGCCTCCGGTGACCGCCGTCGTACCCGCACCTCCGTCACCGTCCAACCTCTCTACGACGGCGACCGCTTCTTCACGTACGCAGAGTTAATCTCCGCCATCGATTCCCTCGCTTCGCAGCTCCGTCGCAATAACCTTAATTCAGCTTCATCAGAGCTCAGACCGAGAATTCTCGGCATATATATGCCGCCGTCGGTGGAATACATAGTCTCCGTACTCTCGGTTTTGAGGTGCGGCGAGGCGTTTCTGCCTCTGGACCCGTCCTGGCCAAAACAGAGGCTGCTCTCCGTCGTTGCTTCAGCCAATGTCGACCTAATTATCACCTGCACGACGCCGTTCGGTTACGAATTGGACTCCAATTGGGTGGAAAAGAATTCCAATCGTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGAGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTCTTCTGTTATGTAATGTACACTTCGGGCTCGACGGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTCTCGATTTTCTACAGGCTTATTCTATCAATAGACTTACTGCTGTTCCATCACTGATGAGAGTAATCCTTCCAGCTTTGCAAGGTCGAGACTACCAGCAGCTTGTAAGTTCACTGAATTTGTTAGTGCTAAGCGGTGAAGTTTTGCCTTTATCGTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGTTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAGTAATGGTGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCGATCATATTTTCAAACTGGTGATGTCGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGGACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGAACATACATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCATAATGGTCATGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTGGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATAATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAGATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTTGATTATGAATCCGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAATCTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTATGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGCAAAGACCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGTGGTTCCATCCAGTGGGAGTTACAACTTGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGTGACTTTACTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGTTGGACTTTCCAAACGTCTGGTGAGGTAAAGTCACAGCCGGTTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCGTAACTTGTATGCGCTAGATTATAAAAATCATTGCTGTGTTTATCAGCTTCCATGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGAGGCCAAATGACAGCCATTTCAGTAACGGCATTCCCATTTTCTATTATGTGGGTGCATGAATTAGAAGTGCCAGTATTTGGGTCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAATACAAGTGGATCTATTATTTGGAGGTATAGAACTGCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCGAACTCGAATCGGGAGGTTTACTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCCGGAAGCATGGTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGGCAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1124

Amino Acids

124.13

Weight (kDa)

5.55

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 41 - 400 4.7e-52 AMP-binding enzyme
AMP-binding_C PF13193 467 - 544 2.7e-09 AMP-binding enzyme C-terminal domain
PQQ_2 PF13360 760 - 869 3.7e-08 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 767 - 1117 1.1e-102 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 909 - 1116 2.2e-10 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1496, 1598, 2652
AarI CACCTGC 2 cut(s) 410, 2169
AasI GACNNNNNNGTC 1 cut(s) 1281
Acc36I ACCTGC 2 cut(s) 410, 2169
AccB7I CCANNNNNTGG 5 cut(s) 659, 2129, 2418, 2687, 2831
AccI GTMKAC 3 cut(s) 387, 1099, 1619
AccIII TCCGGA 1 cut(s) 3173
AclWI GGATC 6 cut(s) 2298, 2311, 2374, 2923, 3068, 3081
AcoI YGGCCR 2 cut(s) 344, 1585
AcsI RAATTY 8 cut(s) 246, 451, 716, 910, 1161, 3232, 3248, 3309
AcuI CTGAAG 3 cut(s) 360, 1782, 2091
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 7 cut(s) 103, 158, 294, 563, 1030, 1146, 2116
AfiI CCNNNNNNNGG 9 cut(s) 659, 1109, 2129, 2418, 2687, 2831, 3115, 3147, 3151
AflIII ACRYGT 3 cut(s) 1977, 2165, 2440
AhlI ACTAGT 2 cut(s) 1622, 2117
AjiI CACGTC 1 cut(s) 2441
AjnI CCWGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
AleI CACNNNNGTG 1 cut(s) 1013
AloI GAACNNNNNNTCC 2 cut(s) 3168, 3200
Alw21I GWGCWC 4 cut(s) 237, 1010, 3108, 3342
Alw26I GTCTC 7 cut(s) 292, 876, 1061, 1067, 1181, 2709, 2841
Alw44I GTGCAC 1 cut(s) 3338
AlwI GGATC 6 cut(s) 2298, 2311, 2374, 2923, 3068, 3081
AlwNI CAGNNNCTG 2 cut(s) 358, 1250
Aor13HI TCCGGA 1 cut(s) 3173
AoxI GGCC 5 cut(s) 344, 1585, 1734, 2755, 2945
ApaLI GTGCAC 1 cut(s) 3338
ApoI RAATTY 8 cut(s) 246, 451, 716, 910, 1161, 3232, 3248, 3309
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 973, 1005
Asp700I GAANNNNTTC 3 cut(s) 785, 1608, 2407
AspLEI GCGC 1 cut(s) 2644
AspS9I GGNCC 4 cut(s) 334, 981, 1357, 2945
AvaII GGWCC 3 cut(s) 334, 981, 1357
BaeGI GKGCMC 1 cut(s) 3342
BalI TGGCCA 2 cut(s) 346, 1587
BamHI GGATCC 2 cut(s) 2303, 3073
BanII GRGCYC 2 cut(s) 237, 575
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 1519
Bbv12I GWGCWC 4 cut(s) 237, 1010, 3108, 3342
BceAI ACGGC 5 cut(s) 81, 154, 253, 397, 2796
BcgI CGANNNNNNTGC 4 cut(s) 185, 219, 3295, 3329
BciT130I CCWGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
BciVI GTATCC 3 cut(s) 660, 1187, 2145
BclI TGATCA 2 cut(s) 703, 3124
BcoDI GTCTC 7 cut(s) 292, 876, 1061, 1067, 1181, 2709, 2841
BcuI ACTAGT 2 cut(s) 1622, 2117
BfaI CTAG 8 cut(s) 1623, 1901, 2004, 2040, 2118, 2238, 2645, 3373
BfmI CTRYAG 2 cut(s) 806, 1104
BfuAI ACCTGC 2 cut(s) 410, 2169
BfuI GTATCC 3 cut(s) 660, 1187, 2145
BglII AGATCT 1 cut(s) 1532
BlpI GCTNAGC 1 cut(s) 923
BmcAI AGTACT 1 cut(s) 2116
Bme1390I CCNGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
Bme18I GGWCC 3 cut(s) 334, 981, 1357
BmgBI CACGTC 1 cut(s) 2441
BmgT120I GGNCC 4 cut(s) 334, 981, 1357, 2945
BmiI GGNNCC 5 cut(s) 336, 2305, 2410, 2962, 3075
BmrFI CCNGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
BmsI GCATC 5 cut(s) 627, 1429, 2327, 2483, 3196
BoxI GACNNNNGTC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 1519
BplI GAGNNNNNCTC 4 cut(s) 155, 187, 3036, 3068
BpmI CTGGAG 3 cut(s) 1227, 2772, 2979
Bpu10I CCTNAGC 1 cut(s) 3110
Bpu1102I GCTNAGC 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 2133
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 3 cut(s) 1181, 2709, 2841
BsaJI CCNNGG 7 cut(s) 480, 977, 2138, 2211, 3268, 3360, 3361
BsaWI WCCGGW 2 cut(s) 86, 3173
Bsc4I CCNNNNNNNGG 9 cut(s) 659, 1109, 2129, 2418, 2687, 2831, 3115, 3147, 3151
Bse118I RCCGGY 1 cut(s) 2581
Bse1I ACTGG 7 cut(s) 742, 1260, 1306, 1575, 2416, 2755, 2825
Bse3DI GCAATG 4 cut(s) 1189, 1581, 2659, 3345
Bse8I GATNNNNATC 1 cut(s) 2133
BseAI TCCGGA 1 cut(s) 3173
BseBI CCWGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 7 cut(s) 480, 977, 2138, 2211, 3268, 3360, 3361
BseGI GGATG 8 cut(s) 642, 1423, 1557, 2412, 2498, 2890, 3211, 3334
BseJI GATNNNNATC 1 cut(s) 2133
BseLI CCNNNNNNNGG 9 cut(s) 659, 1109, 2129, 2418, 2687, 2831, 3115, 3147, 3151
BseMI GCAATG 4 cut(s) 1189, 1581, 2659, 3345
BseMII CTCAG 3 cut(s) 250, 2492, 3101
BseNI ACTGG 7 cut(s) 742, 1260, 1306, 1575, 2416, 2755, 2825
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 3342
BseYI CCCAGC 1 cut(s) 2942
BsgI GTGCAG 2 cut(s) 388, 1803
Bsh1285I CGRYCG 2 cut(s) 145, 3145
BshFI GGCC 5 cut(s) 346, 1587, 1736, 2757, 2947
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 2 cut(s) 145, 3145
BsiHKAI GWGCWC 4 cut(s) 237, 1010, 3108, 3342
BsiSI CCGG 3 cut(s) 87, 2582, 3174
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 34, 2237
BslI CCNNNNNNNGG 9 cut(s) 659, 1109, 2129, 2418, 2687, 2831, 3115, 3147, 3151
BsmAI GTCTC 7 cut(s) 292, 876, 1061, 1067, 1181, 2709, 2841
BsmBI CGTCTC 1 cut(s) 1067
BsmFI GGGAC 2 cut(s) 34, 2237
BsmI GAATGC 1 cut(s) 2783
BsnI GGCC 5 cut(s) 346, 1587, 1736, 2757, 2947
Bso31I GGTCTC 3 cut(s) 1181, 2709, 2841
Bsp1286I GDGCHC 5 cut(s) 237, 575, 1010, 3108, 3342
Bsp13I TCCGGA 1 cut(s) 3173
Bsp1407I TGTACA 2 cut(s) 561, 1028
Bsp143I GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
Bsp1720I GCTNAGC 1 cut(s) 923
Bsp19I CCATGG 2 cut(s) 480, 2138
BspANI GGCC 5 cut(s) 346, 1587, 1736, 2757, 2947
BspCNI CTCAG 3 cut(s) 249, 2491, 3102
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 1 cut(s) 3173
BspLI GGNNCC 5 cut(s) 336, 2305, 2410, 2962, 3075
BspMI ACCTGC 2 cut(s) 410, 2169
BspPI GGATC 6 cut(s) 2298, 2311, 2374, 2923, 3068, 3081
BspQI GCTCTTC 3 cut(s) 551, 1902, 2985
BspTNI GGTCTC 3 cut(s) 1181, 2709, 2841
BsrDI GCAATG 4 cut(s) 1189, 1581, 2659, 3345
BsrFI RCCGGY 1 cut(s) 2581
BsrGI TGTACA 2 cut(s) 561, 1028
BsrI ACTGG 7 cut(s) 742, 1260, 1306, 1575, 2416, 2755, 2825
BssAI RCCGGY 1 cut(s) 2581
BssECI CCNNGG 7 cut(s) 480, 977, 2138, 2211, 3268, 3360, 3361
BssMI GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
BssNAI GTATAC 1 cut(s) 1100
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 3 cut(s) 480, 977, 2138
Bst1107I GTATAC 1 cut(s) 1100
Bst2UI CCWGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
Bst4CI ACNGT 6 cut(s) 121, 505, 1231, 2025, 2594, 2630
Bst6I CTCTTC 5 cut(s) 551, 1758, 1902, 2985, 3289
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 1826
BstAUI TGTACA 2 cut(s) 561, 1028
BstBAI YACGTR 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 2965
BstDEI CTNAG 6 cut(s) 236, 722, 923, 1881, 2478, 3110
BstDSI CCRYGG 2 cut(s) 480, 2138
BstEII GGTNACC 1 cut(s) 89
BstF5I GGATG 8 cut(s) 642, 1423, 1557, 2412, 2498, 2890, 3211, 3334
BstHHI GCGC 1 cut(s) 2644
BstKTI GATC 9 cut(s) 706, 1289, 1535, 2306, 2369, 2610, 2918, 3076, 3127
BstMAI GTCTC 7 cut(s) 292, 876, 1061, 1067, 1181, 2709, 2841
BstMBI GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
BstMCI CGRYCG 2 cut(s) 145, 3145
BstMWI GCNNNNNNNGC 4 cut(s) 1378, 1649, 1826, 3177
BstNI CCWGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
BstNSI RCATGY 3 cut(s) 1981, 2169, 2894
BstPAI GACNNNNGTC 1 cut(s) 96
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
BstSFI CTRYAG 2 cut(s) 806, 1104
BstSLI GKGCMC 1 cut(s) 3342
BstV2I GAAGAC 1 cut(s) 1519
BstX2I RGATCY 4 cut(s) 1532, 2303, 2915, 3073
BstXI CCANNNNNNTGG 2 cut(s) 442, 2561
BstYI RGATCY 4 cut(s) 1532, 2303, 2915, 3073
BstZ17I GTATAC 1 cut(s) 1100
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 3 cut(s) 660, 1187, 2145
BsuRI GGCC 5 cut(s) 346, 1587, 1736, 2757, 2947
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 2 cut(s) 480, 2138
BtrI CACGTC 1 cut(s) 2441
BtsCI GGATG 8 cut(s) 642, 1423, 1557, 2412, 2498, 2890, 3211, 3334
BtsI GCAGTG 1 cut(s) 3340
BtsIMutI CAGTG 7 cut(s) 510, 845, 905, 1227, 1568, 2423, 3340
BveI ACCTGC 2 cut(s) 410, 2169
Cac8I GCNNGC 1 cut(s) 2965
CaiI CAGNNNCTG 2 cut(s) 358, 1250
CfoI GCGC 1 cut(s) 2644
Cfr10I RCCGGY 1 cut(s) 2581
Cfr13I GGNCC 4 cut(s) 334, 981, 1357, 2945
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 3 cut(s) 36, 418, 2324
Csp6I GTAC 7 cut(s) 102, 157, 293, 562, 1029, 1145, 2115
CviQI GTAC 7 cut(s) 102, 157, 293, 562, 1029, 1145, 2115
DdeI CTNAG 6 cut(s) 236, 722, 923, 1881, 2478, 3110
DpnI GATC 9 cut(s) 705, 1288, 1534, 2305, 2368, 2609, 2917, 3075, 3126
DpnII GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
DrdI GACNNNNNNGTC 1 cut(s) 1281
DseDI GACNNNNNNGTC 1 cut(s) 1281
EaeI YGGCCR 2 cut(s) 344, 1585
Eam1104I CTCTTC 5 cut(s) 551, 1758, 1902, 2985, 3289
EarI CTCTTC 5 cut(s) 551, 1758, 1902, 2985, 3289
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 480, 977, 2138
Eco147I AGGCCT 1 cut(s) 1736
Eco24I GRGCYC 2 cut(s) 237, 575
Eco31I GGTCTC 3 cut(s) 1181, 2709, 2841
Eco32I GATATC 1 cut(s) 2350
Eco47I GGWCC 3 cut(s) 334, 981, 1357
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 3 cut(s) 360, 1782, 2091
Eco91I GGTNACC 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 981
EcoO65I GGTNACC 1 cut(s) 89
EcoRI GAATTC 2 cut(s) 246, 451
EcoRII CCWGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
EcoRV GATATC 1 cut(s) 2350
EcoT14I CCWWGG 3 cut(s) 480, 977, 2138
EcoT22I ATGCAT 1 cut(s) 2162
EcoT38I GRGCYC 2 cut(s) 237, 575
ErhI CCWWGG 3 cut(s) 480, 977, 2138
Esp3I CGTCTC 1 cut(s) 1067
FalI AAGNNNNNCTT 4 cut(s) 705, 737, 1674, 1706
FaqI GGGAC 2 cut(s) 34, 2237
FauI CCCGC 1 cut(s) 113
FauNDI CATATG 2 cut(s) 2540, 2623
FbaI TGATCA 2 cut(s) 703, 3124
FblI GTMKAC 3 cut(s) 387, 1099, 1619
FokI GGATG 8 cut(s) 649, 1410, 1564, 2399, 2505, 2897, 3218, 3341
FriOI GRGCYC 2 cut(s) 237, 575
FspBI CTAG 8 cut(s) 1623, 1901, 2004, 2040, 2118, 2238, 2645, 3373
GlaI GCGC 1 cut(s) 2643
GsaI CCCAGC 1 cut(s) 2946
GsuI CTGGAG 3 cut(s) 1227, 2772, 2979
HaeIII GGCC 5 cut(s) 346, 1587, 1736, 2757, 2947
HapII CCGG 3 cut(s) 87, 2582, 3174
HgaI GACGC 3 cut(s) 36, 418, 2324
HhaI GCGC 1 cut(s) 2644
Hin1I GRCGYC 1 cut(s) 410
Hin6I GCGC 1 cut(s) 2642
HinP1I GCGC 1 cut(s) 2642
HincII GTYRAC 6 cut(s) 388, 1080, 1558, 1636, 2296, 2314
HindII GTYRAC 6 cut(s) 388, 1080, 1558, 1636, 2296, 2314
HindIII AAGCTT 2 cut(s) 694, 1883
HpaI GTTAAC 1 cut(s) 1080
HpaII CCGG 3 cut(s) 87, 2582, 3174
Hpy188I TCNGA 9 cut(s) 232, 239, 1204, 1532, 1774, 1951, 2856, 3124, 3189
Hpy99I CGWCG 7 cut(s) 102, 140, 207, 274, 371, 412, 580
HpyAV CCTTC 8 cut(s) 8, 872, 1148, 1344, 1348, 2037, 2429, 3209
HpyCH4III ACNGT 6 cut(s) 121, 505, 1231, 2025, 2594, 2630
HpyCH4IV ACGT 4 cut(s) 155, 1076, 2440, 2558
HpyF10VI GCNNNNNNNGC 4 cut(s) 1378, 1649, 1826, 3177
HpyF3I CTNAG 6 cut(s) 236, 722, 923, 1881, 2478, 3110
HpySE526I ACGT 4 cut(s) 155, 1076, 2440, 2558
Hsp92I GRCGYC 1 cut(s) 410
HspAI GCGC 1 cut(s) 2642
Kpn2I TCCGGA 1 cut(s) 3173
Ksp22I TGATCA 2 cut(s) 703, 3124
KspAI GTTAAC 1 cut(s) 1080
Kzo9I GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
LguI GCTCTTC 3 cut(s) 551, 1902, 2985
LmnI GCTCC 5 cut(s) 204, 1005, 1222, 2960, 3176
LweI GCATC 5 cut(s) 627, 1429, 2327, 2483, 3196
MaeI CTAG 8 cut(s) 1623, 1901, 2004, 2040, 2118, 2238, 2645, 3373
MaeII ACGT 4 cut(s) 155, 1076, 2440, 2558
MalI GATC 9 cut(s) 705, 1288, 1534, 2305, 2368, 2609, 2917, 3075, 3126
MboI GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
MfeI CAATTG 3 cut(s) 436, 1092, 2603
MflI RGATCY 4 cut(s) 1532, 2303, 2915, 3073
MhlI GDGCHC 5 cut(s) 237, 575, 1010, 3108, 3342
MlsI TGGCCA 2 cut(s) 346, 1587
MluNI TGGCCA 2 cut(s) 346, 1587
MlyI GAGTC 2 cut(s) 425, 503
MmeI TCCRAC 6 cut(s) 148, 1529, 1895, 2470, 2525, 3240
Mox20I TGGCCA 2 cut(s) 346, 1587
Mph1103I ATGCAT 1 cut(s) 2162
MroI TCCGGA 1 cut(s) 3173
MroXI GAANNNNTTC 3 cut(s) 785, 1608, 2407
MscI TGGCCA 2 cut(s) 346, 1587
MslI CAYNNNNRTG 5 cut(s) 260, 1013, 1329, 1428, 2664
Msp20I TGGCCA 2 cut(s) 346, 1587
MspI CCGG 3 cut(s) 87, 2582, 3174
MspR9I CCNGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
MunI CAATTG 3 cut(s) 436, 1092, 2603
Mva1269I GAATGC 1 cut(s) 2783
MvaI CCWGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
MwoI GCNNNNNNNGC 4 cut(s) 1378, 1649, 1826, 3177
NcoI CCATGG 2 cut(s) 480, 2138
NdeI CATATG 2 cut(s) 2540, 2623
NdeII GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
NlaIV GGNNCC 5 cut(s) 336, 2305, 2410, 2962, 3075
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 6 cut(s) 89, 115, 499, 1331, 2468, 2574
NsiI ATGCAT 1 cut(s) 2162
NspI RCATGY 3 cut(s) 1981, 2169, 2894
OliI CACNNNNGTG 1 cut(s) 1013
PaqCI CACCTGC 2 cut(s) 410, 2169
PasI CCCWGGG 1 cut(s) 3361
PceI AGGCCT 1 cut(s) 1736
PciI ACATGT 2 cut(s) 1977, 2165
PciSI GCTCTTC 3 cut(s) 551, 1902, 2985
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PctI GAATGC 1 cut(s) 2783
PdmI GAANNNNTTC 3 cut(s) 785, 1608, 2407
Pfl23II CGTACG 1 cut(s) 156
PflMI CCANNNNNTGG 5 cut(s) 659, 2129, 2418, 2687, 2831
PfoI TCCNGGA 1 cut(s) 2517
PleI GAGTC 2 cut(s) 425, 502
PpsI GAGTC 2 cut(s) 425, 502
Ppu21I YACGTR 1 cut(s) 156
PpuMI RGGWCCY 1 cut(s) 981
PscI ACATGT 2 cut(s) 1977, 2165
PshAI GACNNNNGTC 1 cut(s) 96
PsiI TTATAA 3 cut(s) 1496, 1598, 2652
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 981
Psp6I CCWGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
PspEI GGTNACC 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 2942
PspGI CCWGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 5 cut(s) 336, 2305, 2410, 2962, 3075
PspPI GGNCC 4 cut(s) 334, 981, 1357, 2945
PspPPI RGGWCCY 1 cut(s) 981
PstNI CAGNNNCTG 2 cut(s) 358, 1250
PsuI RGATCY 4 cut(s) 1532, 2303, 2915, 3073
RsaI GTAC 7 cut(s) 103, 158, 294, 563, 1030, 1146, 2116
RsaNI GTAC 7 cut(s) 102, 157, 293, 562, 1029, 1145, 2115
RseI CAYNNNNRTG 5 cut(s) 260, 1013, 1329, 1428, 2664
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 3 cut(s) 551, 1902, 2985
Sau3AI GATC 9 cut(s) 703, 1286, 1532, 2303, 2366, 2607, 2915, 3073, 3124
Sau96I GGNCC 4 cut(s) 334, 981, 1357, 2945
ScaI AGTACT 1 cut(s) 2116
SchI GAGTC 2 cut(s) 425, 503
ScrFI CCNGG 7 cut(s) 343, 1584, 2212, 2345, 2519, 3270, 3362
SduI GDGCHC 5 cut(s) 237, 575, 1010, 3108, 3342
SfaNI GCATC 5 cut(s) 627, 1429, 2327, 2483, 3196
SfcI CTRYAG 2 cut(s) 806, 1104
SinI GGWCC 3 cut(s) 334, 981, 1357
SmiMI CAYNNNNRTG 5 cut(s) 260, 1013, 1329, 1428, 2664
SpeI ACTAGT 2 cut(s) 1622, 2117
SseBI AGGCCT 1 cut(s) 1736
SspI AATATT 2 cut(s) 1527, 2866
SspMI CTAG 8 cut(s) 1623, 1901, 2004, 2040, 2118, 2238, 2645, 3373
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 1736
StyD4I CCNGG 7 cut(s) 341, 1582, 2210, 2343, 2517, 3268, 3360
StyI CCWWGG 3 cut(s) 480, 977, 2138
TaaI ACNGT 6 cut(s) 121, 505, 1231, 2025, 2594, 2630
TaiI ACGT 4 cut(s) 158, 1079, 2443, 2561
TaqI TCGA 9 cut(s) 180, 387, 575, 798, 880, 1285, 3030, 3036, 3099
TaqII GACCGA 1 cut(s) 256
TatI WGTACW 4 cut(s) 561, 1028, 1144, 2114
TauI GCSGC 3 cut(s) 268, 315, 2237
TscAI CASTG 7 cut(s) 510, 852, 912, 1234, 1575, 2423, 3347
TseFI GTSAC 6 cut(s) 89, 115, 499, 1331, 2468, 2574
Tsp45I GTSAC 6 cut(s) 89, 115, 499, 1331, 2468, 2574
TspGWI ACGGA 5 cut(s) 103, 191, 280, 355, 1025
TspRI CASTG 7 cut(s) 510, 852, 912, 1234, 1575, 2423, 3347
Van91I CCANNNNNTGG 5 cut(s) 659, 2129, 2418, 2687, 2831
VneI GTGCAC 1 cut(s) 3338
VpaK11BI GGWCC 3 cut(s) 334, 981, 1357
XapI RAATTY 8 cut(s) 246, 451, 716, 910, 1161, 3232, 3248, 3309
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 3 cut(s) 1981, 2169, 2894
XcmI CCANNNNNNNNNTGG 1 cut(s) 1688
XmiI GTMKAC 3 cut(s) 387, 1099, 1619
XmnI GAANNNNTTC 3 cut(s) 785, 1608, 2407
XspI CTAG 8 cut(s) 1623, 1901, 2004, 2040, 2118, 2238, 2645, 3373
ZrmI AGTACT 1 cut(s) 2116
Zsp2I ATGCAT 1 cut(s) 2162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.