Rh1AG152200

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001590
Physical Location & Seq
Reverse (-)
28486043 .. 28495990
9948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG152200.1

Sequence Viewer

Length: 3135 bp
ATGCTGCCGTCGGTGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTGCGGCGAGGCGTTTCTGCCTCTGGACCCGTCCTGGCCAAAACAGAGGCTGCTCTCCGTCGTTGCTTCGGCCAATGTCGACCTAATTATCACCTGCACGACGCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATTCCAATCGTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGGGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGACGGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCACTGAATGGAGAGAAGGTTTTGTTGTTCAAGGCAGCAATAAGCTTTGTTGATCACTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTCTCGATTTTCTACAGGCTTATTCTATCAATAGACTTACTGCTGTTCCATCACTGATGAGAATAATCCTTCCAGCTTTGCAAGGTCGAGACTACCAGCGGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGTGGTGAAGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGTTAACAAGTGTTCCAATTGGTATACCTATAGTTGGTTGTGATGTTGTGCTTGTTAGTGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCGATCATATTTTCAAACTGGTGATTTCGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGGACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGAACATACATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCATAATGGTCAAGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTGGCAAAACATATCCAAGATGAGCTTGGTCACATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATAATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAGATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTGGATTCTGAATCCGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTATGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGTGGTTTCATCCAGTGGGAGTTACAACTCGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGTGACTTTACTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGTTGGACTTTCCAAACGTCTGGTGAGGTAAAGTCACAGCCGGTTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCGTAACTTGTATGCGCTAGATTATAAAAATCAATGCTGTGTTTATCAGCTTCCATGTGGTGGGAGTATCTGTGGGTCCCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGAGGCCAAATGACAGCCATTTCAGTAACGGCATTCCCATTTTCTATTATGTGGGTGCATGAATTAGAAGTGCCAGTATTTGGGTCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAATACAAGTGGATCTATTATTTGGAGGTATAGAACTGCTGGGCCGGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCAAACTCGAATCGGGAGGTTTACTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCCGGAAGCATGGTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGGTGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTCTCTACAGGAACAATGAGTTGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1044

Amino Acids

114.99

Weight (kDa)

5.33

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 3 - 313 1.1e-44 AMP-binding enzyme
AMP-binding_C PF13193 380 - 457 1.8e-09 AMP-binding enzyme C-terminal domain
PQQ_2 PF13360 673 - 782 1.9e-07 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 680 - 1030 1.4e-101 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 822 - 1030 9.9e-11 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1235, 1337, 2391
AarI CACCTGC 2 cut(s) 149, 1908
AasI GACNNNNNNGTC 1 cut(s) 1020
Acc36I ACCTGC 2 cut(s) 149, 1908
AccB7I CCANNNNNTGG 4 cut(s) 398, 1868, 2426, 2570
AccI GTMKAC 3 cut(s) 126, 838, 1358
AccIII TCCGGA 1 cut(s) 2912
AciI CCGC 6 cut(s) 51, 631, 1068, 1099, 1973, 2884
AclWI GGATC 6 cut(s) 2037, 2050, 2113, 2662, 2807, 2820
AcoI YGGCCR 3 cut(s) 83, 117, 1324
AcsI RAATTY 7 cut(s) 190, 455, 649, 900, 2971, 2987, 3048
AcuI CTGAAG 2 cut(s) 1521, 1830
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 4 cut(s) 302, 769, 885, 1855
AfiI CCNNNNNNNGG 8 cut(s) 398, 848, 1868, 2426, 2570, 2854, 2886, 2890
AflIII ACRYGT 3 cut(s) 1716, 1904, 2179
AhlI ACTAGT 2 cut(s) 1361, 1856
AjiI CACGTC 1 cut(s) 2180
AjnI CCWGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
AleI CACNNNNGTG 1 cut(s) 752
AloI GAACNNNNNNTCC 2 cut(s) 2907, 2939
Alw21I GWGCWC 4 cut(s) 36, 749, 2847, 3081
Alw26I GTCTC 7 cut(s) 31, 615, 800, 806, 920, 2580, 3109
Alw44I GTGCAC 1 cut(s) 3077
AlwI GGATC 6 cut(s) 2037, 2050, 2113, 2662, 2807, 2820
AlwNI CAGNNNCTG 2 cut(s) 97, 989
Aor13HI TCCGGA 1 cut(s) 2912
AoxI GGCC 7 cut(s) 83, 117, 1324, 1473, 2080, 2494, 2684
ApaLI GTGCAC 1 cut(s) 3077
ApeKI GCWGC 9 cut(s) 4, 97, 259, 425, 1120, 1181, 1553, 1556, 2195
ApoI RAATTY 7 cut(s) 190, 455, 649, 900, 2971, 2987, 3048
ArsI GACNNNNNNTTYG 2 cut(s) 712, 744
Asp700I GAANNNNTTC 2 cut(s) 524, 1347
AspLEI GCGC 1 cut(s) 2383
AspS9I GGNCC 5 cut(s) 73, 720, 1096, 2442, 2684
AsuHPI GGTGA 9 cut(s) 130, 680, 773, 1055, 1082, 1966, 2052, 2219, 2315
AvaII GGWCC 4 cut(s) 73, 720, 1096, 2442
BaeGI GKGCMC 1 cut(s) 3081
BalI TGGCCA 2 cut(s) 85, 1326
BamHI GGATCC 2 cut(s) 2042, 2812
BanII GRGCYC 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 1258
Bbv12I GWGCWC 4 cut(s) 36, 749, 2847, 3081
BbvI GCAGC 8 cut(s) 84, 271, 437, 1132, 1168, 1540, 1543, 2207
BccI CCATC 9 cut(s) 245, 589, 1285, 1286, 1624, 1862, 2591, 2618, 2744
BceAI ACGGC 2 cut(s) 136, 2535
BcgI CGANNNNNNTGC 2 cut(s) 3034, 3068
BciT130I CCWGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
BciVI GTATCC 3 cut(s) 399, 926, 1884
BclI TGATCA 2 cut(s) 442, 2863
BcoDI GTCTC 7 cut(s) 31, 615, 800, 806, 920, 2580, 3109
BcuI ACTAGT 2 cut(s) 1361, 1856
BfaI CTAG 7 cut(s) 1362, 1640, 1743, 1779, 1857, 1977, 2384
BfmI CTRYAG 3 cut(s) 545, 843, 3108
BfuAI ACCTGC 2 cut(s) 149, 1908
BfuI GTATCC 3 cut(s) 399, 926, 1884
BglII AGATCT 1 cut(s) 1271
BmcAI AGTACT 1 cut(s) 1855
Bme1390I CCNGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
Bme18I GGWCC 4 cut(s) 73, 720, 1096, 2442
BmgBI CACGTC 1 cut(s) 2180
BmgT120I GGNCC 5 cut(s) 73, 720, 1096, 2442, 2684
BmiI GGNNCC 6 cut(s) 75, 2044, 2443, 2444, 2701, 2814
BmrFI CCNGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
BmsI GCATC 5 cut(s) 366, 1168, 2066, 2222, 2935
BpiI GAAGAC 1 cut(s) 1258
BplI GAGNNNNNCTC 2 cut(s) 2775, 2807
BpmI CTGGAG 3 cut(s) 966, 2511, 2718
Bpu10I CCTNAGC 1 cut(s) 2849
BsaBI GATNNNNATC 1 cut(s) 1872
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 3 cut(s) 920, 2580, 3109
BsaJI CCNNGG 6 cut(s) 219, 716, 1877, 1950, 3007, 3099
BsaWI WCCGGW 1 cut(s) 2912
Bsc4I CCNNNNNNNGG 8 cut(s) 398, 848, 1868, 2426, 2570, 2854, 2886, 2890
Bse118I RCCGGY 2 cut(s) 2320, 2686
Bse1I ACTGG 7 cut(s) 481, 999, 1045, 1314, 2155, 2494, 2564
Bse3DI GCAATG 3 cut(s) 928, 1320, 3084
Bse8I GATNNNNATC 1 cut(s) 1872
BseAI TCCGGA 1 cut(s) 2912
BseBI CCWGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
BseDI CCNNGG 6 cut(s) 219, 716, 1877, 1950, 3007, 3099
BseGI GGATG 8 cut(s) 381, 1162, 1296, 2151, 2237, 2629, 2950, 3073
BseJI GATNNNNATC 1 cut(s) 1872
BseLI CCNNNNNNNGG 8 cut(s) 398, 848, 1868, 2426, 2570, 2854, 2886, 2890
BseMI GCAATG 3 cut(s) 928, 1320, 3084
BseMII CTCAG 2 cut(s) 2231, 2840
BseNI ACTGG 7 cut(s) 481, 999, 1045, 1314, 2155, 2494, 2564
BseSI GKGCMC 1 cut(s) 3081
BseXI GCAGC 8 cut(s) 84, 271, 437, 1132, 1168, 1540, 1543, 2207
BseYI CCCAGC 1 cut(s) 2681
BsgI GTGCAG 2 cut(s) 127, 1542
Bsh1285I CGRYCG 1 cut(s) 2884
BshFI GGCC 7 cut(s) 85, 119, 1326, 1475, 2082, 2496, 2686
BsiEI CGRYCG 1 cut(s) 2884
BsiHKAI GWGCWC 4 cut(s) 36, 749, 2847, 3081
BsiSI CCGG 3 cut(s) 2321, 2687, 2913
BslFI GGGAC 2 cut(s) 1976, 2428
BslI CCNNNNNNNGG 8 cut(s) 398, 848, 1868, 2426, 2570, 2854, 2886, 2890
BsmAI GTCTC 7 cut(s) 31, 615, 800, 806, 920, 2580, 3109
BsmBI CGTCTC 1 cut(s) 806
BsmFI GGGAC 2 cut(s) 1976, 2428
BsmI GAATGC 1 cut(s) 2522
BsnI GGCC 7 cut(s) 85, 119, 1326, 1475, 2082, 2496, 2686
Bso31I GGTCTC 3 cut(s) 920, 2580, 3109
Bsp1286I GDGCHC 5 cut(s) 36, 314, 749, 2847, 3081
Bsp13I TCCGGA 1 cut(s) 2912
Bsp1407I TGTACA 2 cut(s) 300, 767
Bsp143I GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
Bsp19I CCATGG 2 cut(s) 219, 1877
BspACI CCGC 6 cut(s) 51, 631, 1068, 1099, 1973, 2884
BspANI GGCC 7 cut(s) 85, 119, 1326, 1475, 2082, 2496, 2686
BspCNI CTCAG 2 cut(s) 2230, 2841
BspEI TCCGGA 1 cut(s) 2912
BspLI GGNNCC 6 cut(s) 75, 2044, 2443, 2444, 2701, 2814
BspMI ACCTGC 2 cut(s) 149, 1908
BspPI GGATC 6 cut(s) 2037, 2050, 2113, 2662, 2807, 2820
BspQI GCTCTTC 2 cut(s) 1641, 2724
BspTNI GGTCTC 3 cut(s) 920, 2580, 3109
BsrDI GCAATG 3 cut(s) 928, 1320, 3084
BsrFI RCCGGY 2 cut(s) 2320, 2686
BsrGI TGTACA 2 cut(s) 300, 767
BsrI ACTGG 7 cut(s) 481, 999, 1045, 1314, 2155, 2494, 2564
BssAI RCCGGY 2 cut(s) 2320, 2686
BssECI CCNNGG 6 cut(s) 219, 716, 1877, 1950, 3007, 3099
BssMI GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
BssNAI GTATAC 1 cut(s) 839
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 3 cut(s) 219, 716, 1877
Bst1107I GTATAC 1 cut(s) 839
Bst2UI CCWGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
Bst4CI ACNGT 5 cut(s) 244, 970, 1764, 2333, 2369
Bst6I CTCTTC 4 cut(s) 1497, 1641, 2724, 3028
BstACI GRCGYC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 2 cut(s) 300, 767
BstC8I GCNNGC 1 cut(s) 2704
BstDEI CTNAG 6 cut(s) 461, 638, 662, 1620, 2217, 2849
BstDSI CCRYGG 2 cut(s) 219, 1877
BstF5I GGATG 8 cut(s) 381, 1162, 1296, 2151, 2237, 2629, 2950, 3073
BstHHI GCGC 1 cut(s) 2383
BstKTI GATC 9 cut(s) 445, 1028, 1274, 2045, 2108, 2349, 2657, 2815, 2866
BstMAI GTCTC 7 cut(s) 31, 615, 800, 806, 920, 2580, 3109
BstMBI GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
BstMCI CGRYCG 1 cut(s) 2884
BstMWI GCNNNNNNNGC 4 cut(s) 1117, 1388, 1565, 2916
BstNI CCWGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
BstNSI RCATGY 3 cut(s) 1720, 1908, 2633
BstSCI CCNGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
BstSFI CTRYAG 3 cut(s) 545, 843, 3108
BstSLI GKGCMC 1 cut(s) 3081
BstV1I GCAGC 8 cut(s) 84, 271, 437, 1132, 1168, 1540, 1543, 2207
BstV2I GAAGAC 1 cut(s) 1258
BstX2I RGATCY 4 cut(s) 1271, 2042, 2654, 2812
BstXI CCANNNNNNTGG 2 cut(s) 181, 2300
BstYI RGATCY 4 cut(s) 1271, 2042, 2654, 2812
BstZ17I GTATAC 1 cut(s) 839
BsuI GTATCC 3 cut(s) 399, 926, 1884
BsuRI GGCC 7 cut(s) 85, 119, 1326, 1475, 2082, 2496, 2686
BtgI CCRYGG 2 cut(s) 219, 1877
BtrI CACGTC 1 cut(s) 2180
BtsCI GGATG 8 cut(s) 381, 1162, 1296, 2151, 2237, 2629, 2950, 3073
BtsI GCAGTG 1 cut(s) 3079
BtsIMutI CAGTG 8 cut(s) 249, 392, 584, 644, 966, 1307, 2162, 3079
BveI ACCTGC 2 cut(s) 149, 1908
Cac8I GCNNGC 1 cut(s) 2704
CaiI CAGNNNCTG 2 cut(s) 97, 989
CfoI GCGC 1 cut(s) 2383
Cfr10I RCCGGY 2 cut(s) 2320, 2686
Cfr13I GGNCC 5 cut(s) 73, 720, 1096, 2442, 2684
CseI GACGC 2 cut(s) 157, 2063
Csp6I GTAC 4 cut(s) 301, 768, 884, 1854
CviQI GTAC 4 cut(s) 301, 768, 884, 1854
DdeI CTNAG 6 cut(s) 461, 638, 662, 1620, 2217, 2849
DpnI GATC 9 cut(s) 444, 1027, 1273, 2044, 2107, 2348, 2656, 2814, 2865
DpnII GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
DrdI GACNNNNNNGTC 1 cut(s) 1020
DseDI GACNNNNNNGTC 1 cut(s) 1020
EaeI YGGCCR 3 cut(s) 83, 117, 1324
Eam1104I CTCTTC 4 cut(s) 1497, 1641, 2724, 3028
EarI CTCTTC 4 cut(s) 1497, 1641, 2724, 3028
Eco130I CCWWGG 3 cut(s) 219, 716, 1877
Eco147I AGGCCT 1 cut(s) 1475
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 3 cut(s) 920, 2580, 3109
Eco32I GATATC 1 cut(s) 2089
Eco47I GGWCC 4 cut(s) 73, 720, 1096, 2442
Eco57I CTGAAG 2 cut(s) 1521, 1830
EcoO109I RGGNCCY 2 cut(s) 720, 2442
EcoRI GAATTC 1 cut(s) 190
EcoRII CCWGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
EcoRV GATATC 1 cut(s) 2089
EcoT14I CCWWGG 3 cut(s) 219, 716, 1877
EcoT22I ATGCAT 1 cut(s) 1901
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 3 cut(s) 219, 716, 1877
Esp3I CGTCTC 1 cut(s) 806
FalI AAGNNNNNCTT 6 cut(s) 444, 476, 1197, 1229, 1413, 1445
FaqI GGGAC 2 cut(s) 1976, 2428
FauNDI CATATG 2 cut(s) 2279, 2362
FbaI TGATCA 2 cut(s) 442, 2863
FblI GTMKAC 3 cut(s) 126, 838, 1358
FokI GGATG 8 cut(s) 388, 1149, 1303, 2138, 2244, 2636, 2957, 3080
FriOI GRGCYC 1 cut(s) 314
FspBI CTAG 7 cut(s) 1362, 1640, 1743, 1779, 1857, 1977, 2384
GlaI GCGC 1 cut(s) 2382
GsaI CCCAGC 1 cut(s) 2685
GsuI CTGGAG 3 cut(s) 966, 2511, 2718
HaeIII GGCC 7 cut(s) 85, 119, 1326, 1475, 2082, 2496, 2686
HapII CCGG 3 cut(s) 2321, 2687, 2913
HgaI GACGC 2 cut(s) 157, 2063
HhaI GCGC 1 cut(s) 2383
Hin1I GRCGYC 1 cut(s) 149
Hin6I GCGC 1 cut(s) 2381
HinP1I GCGC 1 cut(s) 2381
HincII GTYRAC 6 cut(s) 127, 819, 1297, 1375, 2035, 2053
HindII GTYRAC 6 cut(s) 127, 819, 1297, 1375, 2035, 2053
HindIII AAGCTT 2 cut(s) 433, 1622
HpaI GTTAAC 1 cut(s) 819
HpaII CCGG 3 cut(s) 2321, 2687, 2913
HphI GGTGA 9 cut(s) 130, 680, 773, 1055, 1082, 1966, 2052, 2219, 2315
Hpy188I TCNGA 8 cut(s) 943, 1271, 1513, 1684, 1690, 2595, 2863, 2928
Hpy99I CGWCG 4 cut(s) 13, 110, 151, 319
HpyAV CCTTC 8 cut(s) 400, 611, 887, 1083, 1087, 1776, 2168, 2948
HpyCH4III ACNGT 5 cut(s) 244, 970, 1764, 2333, 2369
HpyCH4IV ACGT 3 cut(s) 815, 2179, 2297
HpyF10VI GCNNNNNNNGC 4 cut(s) 1117, 1388, 1565, 2916
HpyF3I CTNAG 6 cut(s) 461, 638, 662, 1620, 2217, 2849
HpySE526I ACGT 3 cut(s) 815, 2179, 2297
Hsp92I GRCGYC 1 cut(s) 149
HspAI GCGC 1 cut(s) 2381
KflI GGGWCCC 1 cut(s) 2442
Kpn2I TCCGGA 1 cut(s) 2912
Ksp22I TGATCA 2 cut(s) 442, 2863
KspAI GTTAAC 1 cut(s) 819
Kzo9I GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
LguI GCTCTTC 2 cut(s) 1641, 2724
LmnI GCTCC 4 cut(s) 744, 961, 2699, 2915
Lsp1109I GCAGC 8 cut(s) 84, 271, 437, 1132, 1168, 1540, 1543, 2207
LweI GCATC 5 cut(s) 366, 1168, 2066, 2222, 2935
MaeI CTAG 7 cut(s) 1362, 1640, 1743, 1779, 1857, 1977, 2384
MaeII ACGT 3 cut(s) 815, 2179, 2297
MalI GATC 9 cut(s) 444, 1027, 1273, 2044, 2107, 2348, 2656, 2814, 2865
MboI GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
MfeI CAATTG 3 cut(s) 175, 831, 2342
MflI RGATCY 4 cut(s) 1271, 2042, 2654, 2812
MhlI GDGCHC 5 cut(s) 36, 314, 749, 2847, 3081
MlsI TGGCCA 2 cut(s) 85, 1326
MluNI TGGCCA 2 cut(s) 85, 1326
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 5 cut(s) 1268, 1634, 2209, 2264, 2979
Mox20I TGGCCA 2 cut(s) 85, 1326
Mph1103I ATGCAT 1 cut(s) 1901
MroI TCCGGA 1 cut(s) 2912
MroXI GAANNNNTTC 2 cut(s) 524, 1347
MscI TGGCCA 2 cut(s) 85, 1326
MslI CAYNNNNRTG 3 cut(s) 752, 1068, 1167
Msp20I TGGCCA 2 cut(s) 85, 1326
MspA1I CMGCKG 1 cut(s) 631
MspI CCGG 3 cut(s) 2321, 2687, 2913
MspR9I CCNGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
MunI CAATTG 3 cut(s) 175, 831, 2342
Mva1269I GAATGC 1 cut(s) 2522
MvaI CCWGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
MwoI GCNNNNNNNGC 4 cut(s) 1117, 1388, 1565, 2916
NcoI CCATGG 2 cut(s) 219, 1877
NdeI CATATG 2 cut(s) 2279, 2362
NdeII GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
NlaIV GGNNCC 6 cut(s) 75, 2044, 2443, 2444, 2701, 2814
NmuCI GTSAC 5 cut(s) 238, 1070, 1433, 2207, 2313
NsiI ATGCAT 1 cut(s) 1901
NspI RCATGY 3 cut(s) 1720, 1908, 2633
OliI CACNNNNGTG 1 cut(s) 752
PaqCI CACCTGC 2 cut(s) 149, 1908
PceI AGGCCT 1 cut(s) 1475
PciI ACATGT 2 cut(s) 1716, 1904
PciSI GCTCTTC 2 cut(s) 1641, 2724
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 1 cut(s) 2522
PdmI GAANNNNTTC 2 cut(s) 524, 1347
PflMI CCANNNNNTGG 4 cut(s) 398, 1868, 2426, 2570
PfoI TCCNGGA 1 cut(s) 2256
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PpuMI RGGWCCY 2 cut(s) 720, 2442
PscI ACATGT 2 cut(s) 1716, 1904
PsiI TTATAA 3 cut(s) 1235, 1337, 2391
Psp5II RGGWCCY 2 cut(s) 720, 2442
Psp6I CCWGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
PspFI CCCAGC 1 cut(s) 2681
PspGI CCWGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
PspN4I GGNNCC 6 cut(s) 75, 2044, 2443, 2444, 2701, 2814
PspPI GGNCC 5 cut(s) 73, 720, 1096, 2442, 2684
PspPPI RGGWCCY 2 cut(s) 720, 2442
PstNI CAGNNNCTG 2 cut(s) 97, 989
PsuI RGATCY 4 cut(s) 1271, 2042, 2654, 2812
RsaI GTAC 4 cut(s) 302, 769, 885, 1855
RsaNI GTAC 4 cut(s) 301, 768, 884, 1854
RseI CAYNNNNRTG 3 cut(s) 752, 1068, 1167
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 2 cut(s) 1641, 2724
Sau3AI GATC 9 cut(s) 442, 1025, 1271, 2042, 2105, 2346, 2654, 2812, 2863
Sau96I GGNCC 5 cut(s) 73, 720, 1096, 2442, 2684
ScaI AGTACT 1 cut(s) 1855
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 7 cut(s) 82, 1323, 1951, 2084, 2258, 3009, 3101
SduI GDGCHC 5 cut(s) 36, 314, 749, 2847, 3081
SfaNI GCATC 5 cut(s) 366, 1168, 2066, 2222, 2935
SfcI CTRYAG 3 cut(s) 545, 843, 3108
SinI GGWCC 4 cut(s) 73, 720, 1096, 2442
SmiMI CAYNNNNRTG 3 cut(s) 752, 1068, 1167
SpeI ACTAGT 2 cut(s) 1361, 1856
SseBI AGGCCT 1 cut(s) 1475
SsiI CCGC 6 cut(s) 51, 631, 1068, 1099, 1973, 2884
SspI AATATT 2 cut(s) 1266, 2605
SspMI CTAG 7 cut(s) 1362, 1640, 1743, 1779, 1857, 1977, 2384
StuI AGGCCT 1 cut(s) 1475
StyD4I CCNGG 7 cut(s) 80, 1321, 1949, 2082, 2256, 3007, 3099
StyI CCWWGG 3 cut(s) 219, 716, 1877
TaaI ACNGT 5 cut(s) 244, 970, 1764, 2333, 2369
TaiI ACGT 3 cut(s) 818, 2182, 2300
TaqI TCGA 8 cut(s) 126, 314, 537, 619, 1024, 2172, 2775, 2838
TatI WGTACW 4 cut(s) 300, 767, 883, 1853
TauI GCSGC 3 cut(s) 54, 634, 1976
TscAI CASTG 8 cut(s) 249, 399, 591, 651, 973, 1314, 2162, 3086
TseFI GTSAC 5 cut(s) 238, 1070, 1433, 2207, 2313
TseI GCWGC 9 cut(s) 4, 97, 259, 425, 1120, 1181, 1553, 1556, 2195
Tsp45I GTSAC 5 cut(s) 238, 1070, 1433, 2207, 2313
TspGWI ACGGA 3 cut(s) 19, 94, 764
TspRI CASTG 8 cut(s) 249, 399, 591, 651, 973, 1314, 2162, 3086
Van91I CCANNNNNTGG 4 cut(s) 398, 1868, 2426, 2570
VneI GTGCAC 1 cut(s) 3077
VpaK11BI GGWCC 4 cut(s) 73, 720, 1096, 2442
XapI RAATTY 7 cut(s) 190, 455, 649, 900, 2971, 2987, 3048
XbaI TCTAGA 1 cut(s) 1639
XceI RCATGY 3 cut(s) 1720, 1908, 2633
XcmI CCANNNNNNNNNTGG 1 cut(s) 1427
XmiI GTMKAC 3 cut(s) 126, 838, 1358
XmnI GAANNNNTTC 2 cut(s) 524, 1347
XspI CTAG 7 cut(s) 1362, 1640, 1743, 1779, 1857, 1977, 2384
ZrmI AGTACT 1 cut(s) 1855
Zsp2I ATGCAT 1 cut(s) 1901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.