FvH4_7g31431

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006952
Physical Location & Seq
Forward (+)
22713226 .. 22714800
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31431.t1

Sequence Viewer

Length: 1398 bp
ATGGGAGCAGGTGGCGATAGTAATAGTGTTTCTACCAAAAGAAGAAGTTACTGCAGTACTGTCGTGAATACTAACATTGATGATCTTACTGACAAAGTATTGGCCGACATCCTTTCTAGACTTCCAAGGTCTGATCACCTTTCTAAATACATGTTTGTGTGCAACCGTTGGTGGTATATTTTCATGTCCGACCTATACTTCCGAGGCCTCTATCTACTTCGACAGAAGCAACCACCGGTACCGGCACATACTCTGATCAACAAAAGAGGAGATACCACCCCATTTGAATTGGGTCTTCCTTATTCATCTCCCAAGTTTCTATCTAGGCTTTTCGACGAACTCATGAGTGTCATGTCTCTAACACGCGAGCCAGTCGTGGTAGGGGCGTCTAATGACTTGATTTTGTGCTGCGCAACCGAGTATTTTCAACGTGATTACTTCATTTGCAATCCATTTAGCTTACAATGGATTAAGCTTCCTCCCCCCTCTCGAGTCCTCCATGACGTACCAATTGGGTTCATATGTGATCCCTATTATAGTTATATTAAGAAAGACGACAAAGACAATGTGCATTATATTCCTCGAAGAGAAAAGTGTGCAATCATCGATGATGGCAATGAGGACCTAGTTATTATCAATCACTTGTATAGGTGCAAGGTTGTGAGAATTCTTCCTTACGACGACAAATTTGAAGAAGACGATTCCTCCAAGTTTAAGCTGGATATCTTCTCTTTTGAGACGTGGGAATGGAGAGGGTCGATTATATCATTCCCACGAAGTTTTACTTTCGATAGAATCAACTCGGATAGTCACTTCTCTCACAACGGAATGTTATATTGGTCGAGTACGTGGGAAACTACCAACTTTCTGATAGGGTTAGACCCTTTCATGATCAACGACGCTAACATGAATGCCGATAATTCGAGCGGTTATGAAGGTAGTTACATTAAGTTAACAGTTAAACACCCGGCGTTATACAGTTCAGAAAAACATTACTATCAGATAAAGTGTCTGAGTGTGCGATCGGCCGGAGGTGGAGGGTGTCTGCAAGTGTGTGTCTTAGACTCTTATGACAACAGTATATTGGTTTGGGAGTTGAAAGAAGAGAAAGATCTGATCATTGCGGGAGATGGGGCTGGAATATTGCGTTCGAAAGGAGAAGCTATCAAAGTGCATTGGCAGGAACAAGTGGAGCTGATAGGTTTCGACCCAGATGTCGAGGGTTTGCTGTATGTAAGACGCGACGGAGTTGAGGGAGTTGACGGAGGATCTTGCTATTGTAAGATCAACGTTCGTGCTAGAGAGTATACAAACTTAATAATGGATGGAGGTGTAATTAGTAACAATTGCTTCTTCCCAATAAAAGTGAATCCATGCTGGCCGACACCAGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

466

Amino Acids

53.24

Weight (kDa)

5.55

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 122 - 287 2.1e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1214
Acc16I TGCGCA 1 cut(s) 412
Acc65I GGTACC 1 cut(s) 238
AccB1I GGYRCC 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 927
AccI GTMKAC 1 cut(s) 1307
AccII CGCG 2 cut(s) 366, 1242
AciI CCGC 2 cut(s) 927, 1124
AclI AACGTT 1 cut(s) 1290
AclWI GGATC 2 cut(s) 521, 1276
AcoI YGGCCR 3 cut(s) 102, 1026, 1379
AcsI RAATTY 2 cut(s) 666, 686
AcyI GRCGYC 1 cut(s) 386
AfaI GTAC 4 cut(s) 58, 240, 507, 847
AflIII ACRYGT 1 cut(s) 150
AgeI ACCGGT 1 cut(s) 235
AgsI TTSAA 4 cut(s) 287, 428, 692, 1099
AjiI CACGTC 1 cut(s) 741
AjuI GAANNNNNNNTTGG 2 cut(s) 820, 852
AluBI AGCT 5 cut(s) 459, 475, 718, 1163, 1195
AluI AGCT 5 cut(s) 459, 475, 718, 1163, 1195
Alw26I GTCTC 2 cut(s) 360, 731
AlwI GGATC 2 cut(s) 521, 1276
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 4 cut(s) 102, 205, 1026, 1379
ApeKI GCWGC 1 cut(s) 408
ApoI RAATTY 2 cut(s) 666, 686
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AsiGI ACCGGT 1 cut(s) 235
Asp718I GGTACC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 622
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 1 cut(s) 128
AsuII TTCGAA 1 cut(s) 1151
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 238
BbsI GAAGAC 2 cut(s) 287, 702
BbvI GCAGC 1 cut(s) 395
BccI CCATC 3 cut(s) 605, 1124, 1319
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BclI TGATCA 4 cut(s) 133, 255, 891, 1116
BcnI CCSGG 1 cut(s) 968
BcoDI GTCTC 2 cut(s) 360, 731
BfaI CTAG 4 cut(s) 117, 324, 626, 1299
BfmI CTRYAG 1 cut(s) 52
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 968
Bme18I GGWCC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 489
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 968
BpiI GAAGAC 2 cut(s) 287, 702
Bpu14I TTCGAA 1 cut(s) 1151
BpuMI CCSGG 1 cut(s) 968
Bsa29I ATCGAT 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 125, 202
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 689, 719
Bse118I RCCGGY 2 cut(s) 235, 241
Bse1I ACTGG 2 cut(s) 371, 1388
Bse3DI GCAATG 2 cut(s) 622, 1119
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 125, 202
BseGI GGATG 2 cut(s) 108, 1330
BseMI GCAATG 2 cut(s) 622, 1119
BseMII CTCAG 1 cut(s) 1004
BseNI ACTGG 2 cut(s) 371, 1388
BseRI GAGGAG 1 cut(s) 282
BseX3I CGGCCG 1 cut(s) 1026
BseXI GCAGC 1 cut(s) 395
Bsh1236I CGCG 2 cut(s) 366, 1242
Bsh1285I CGRYCG 2 cut(s) 1025, 1029
BshFI GGCC 4 cut(s) 104, 207, 1028, 1381
BshNI GGYRCC 1 cut(s) 238
BshTI ACCGGT 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 2 cut(s) 1025, 1029
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 4 cut(s) 236, 242, 968, 1029
BsmAI GTCTC 2 cut(s) 360, 731
BsmBI CGTCTC 1 cut(s) 731
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 4 cut(s) 104, 207, 1028, 1381
BsoBI CYCGRG 1 cut(s) 489
Bsp119I TTCGAA 1 cut(s) 1151
BspACI CCGC 2 cut(s) 927, 1124
BspANI GGCC 4 cut(s) 104, 207, 1028, 1381
BspCNI CTCAG 1 cut(s) 1005
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 2 cut(s) 366, 1242
BspHI TCATGA 2 cut(s) 342, 888
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 521, 1276
BspT104I TTCGAA 1 cut(s) 1151
BspT107I GGYRCC 1 cut(s) 238
BsrBI CCGCTC 1 cut(s) 927
BsrDI GCAATG 2 cut(s) 622, 1119
BsrFI RCCGGY 2 cut(s) 235, 241
BsrI ACTGG 2 cut(s) 371, 1388
BssAI RCCGGY 2 cut(s) 235, 241
BssECI CCNNGG 2 cut(s) 125, 202
BssNAI GTATAC 1 cut(s) 1308
BssNI GRCGYC 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 125
Bst1107I GTATAC 1 cut(s) 1308
Bst4CI ACNGT 5 cut(s) 61, 167, 958, 980, 1079
Bst6I CTCTTC 2 cut(s) 580, 1098
BstACI GRCGYC 1 cut(s) 386
BstBAI YACGTR 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 1151
BstC8I GCNNGC 2 cut(s) 368, 1379
BstDEI CTNAG 2 cut(s) 1013, 1060
BstF5I GGATG 2 cut(s) 108, 1330
BstFNI CGCG 2 cut(s) 366, 1242
BstHHI GCGC 1 cut(s) 413
BstMAI GTCTC 2 cut(s) 360, 731
BstMCI CGRYCG 2 cut(s) 1025, 1029
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 966
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 2 cut(s) 366, 1242
BstV1I GCAGC 1 cut(s) 395
BstV2I GAAGAC 2 cut(s) 287, 702
BstX2I RGATCY 2 cut(s) 1111, 1268
BstYI RGATCY 2 cut(s) 1111, 1268
BstZ17I GTATAC 1 cut(s) 1308
BstZI CGGCCG 1 cut(s) 1026
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 4 cut(s) 104, 207, 1028, 1381
BsuTUI ATCGAT 1 cut(s) 606
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 2 cut(s) 108, 1330
Cac8I GCNNGC 2 cut(s) 368, 1379
CciI TCATGA 2 cut(s) 342, 888
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 2 cut(s) 235, 241
Cfr13I GGNCC 1 cut(s) 622
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 3 cut(s) 375, 908, 1248
Csp6I GTAC 4 cut(s) 57, 239, 506, 846
CspAI ACCGGT 1 cut(s) 235
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 8 cut(s) 151, 184, 343, 352, 500, 889, 907, 1374
CviQI GTAC 4 cut(s) 57, 239, 506, 846
DdeI CTNAG 2 cut(s) 1013, 1060
DrdI GACNNNNNNGTC 1 cut(s) 1214
DseDI GACNNNNNNGTC 1 cut(s) 1214
EaeI YGGCCR 3 cut(s) 102, 1026, 1379
EagI CGGCCG 1 cut(s) 1026
Eam1104I CTCTTC 2 cut(s) 580, 1098
EarI CTCTTC 2 cut(s) 580, 1098
EclXI CGGCCG 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 125
Eco147I AGGCCT 1 cut(s) 207
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 622
Eco52I CGGCCG 1 cut(s) 1026
Eco88I CYCGRG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 622
EcoRI GAATTC 1 cut(s) 666
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 125
Esp3I CGTCTC 1 cut(s) 731
FaeI CATG 8 cut(s) 154, 187, 346, 355, 503, 892, 910, 1377
FalI AAGNNNNNCTT 2 cut(s) 769, 801
FatI CATG 8 cut(s) 150, 183, 342, 351, 499, 888, 906, 1373
FauI CCCGC 1 cut(s) 1117
FauNDI CATATG 1 cut(s) 521
FbaI TGATCA 4 cut(s) 133, 255, 891, 1116
FblI GTMKAC 1 cut(s) 1307
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 2 cut(s) 95, 1337
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 4 cut(s) 117, 324, 626, 1299
FspI TGCGCA 1 cut(s) 412
GlaI GCGC 1 cut(s) 412
GluI GCNGC 1 cut(s) 409
HaeIII GGCC 4 cut(s) 104, 207, 1028, 1381
HapII CCGG 4 cut(s) 236, 242, 968, 1029
HgaI GACGC 3 cut(s) 375, 908, 1248
HhaI GCGC 1 cut(s) 413
Hin1I GRCGYC 1 cut(s) 386
Hin1II CATG 8 cut(s) 154, 187, 346, 355, 503, 892, 910, 1377
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HincII GTYRAC 2 cut(s) 954, 1261
HindII GTYRAC 2 cut(s) 954, 1261
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 5 cut(s) 492, 701, 795, 1064, 1369
HpaI GTTAAC 1 cut(s) 954
HpaII CCGG 4 cut(s) 236, 242, 968, 1029
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 3 cut(s) 954, 1261, 1308
Hpy188III TCNNGA 5 cut(s) 64, 117, 343, 489, 889
Hpy8I GTNNAC 3 cut(s) 954, 1261, 1308
Hpy99I CGWCG 4 cut(s) 338, 683, 902, 1247
HpyAV CCTTC 1 cut(s) 929
HpyCH4III ACNGT 5 cut(s) 61, 167, 958, 980, 1079
HpyCH4IV ACGT 5 cut(s) 430, 504, 740, 848, 1290
HpyCH4V TGCA 8 cut(s) 54, 162, 447, 571, 599, 654, 1048, 1174
HpyF3I CTNAG 2 cut(s) 1013, 1060
HpySE526I ACGT 5 cut(s) 430, 504, 740, 848, 1290
Hsp92I GRCGYC 1 cut(s) 386
Hsp92II CATG 8 cut(s) 154, 187, 346, 355, 503, 892, 910, 1377
HspAI GCGC 1 cut(s) 411
KpnI GGTACC 1 cut(s) 242
Ksp22I TGATCA 4 cut(s) 133, 255, 891, 1116
KspAI GTTAAC 1 cut(s) 954
LmnI GCTCC 2 cut(s) 5, 1192
Lsp1109I GCAGC 1 cut(s) 395
MaeI CTAG 4 cut(s) 117, 324, 626, 1299
MaeII ACGT 5 cut(s) 430, 504, 740, 848, 1290
MaeIII GTNAC 4 cut(s) 47, 809, 941, 1340
MbiI CCGCTC 1 cut(s) 927
MboII GAAGA 9 cut(s) 54, 287, 597, 662, 704, 707, 718, 1115, 1345
MfeI CAATTG 2 cut(s) 510, 1345
MflI RGATCY 2 cut(s) 1111, 1268
MluCI AATT 7 cut(s) 287, 510, 666, 686, 919, 1335, 1345
MlyI GAGTC 2 cut(s) 501, 1058
MmeI TCCRAC 1 cut(s) 213
MseI TTAA 7 cut(s) 471, 546, 714, 948, 953, 960, 1316
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 4 cut(s) 236, 242, 968, 1029
MspR9I CCNGG 1 cut(s) 968
MunI CAATTG 2 cut(s) 510, 1345
Mva1269I GAATGC 1 cut(s) 916
MvnI CGCG 2 cut(s) 366, 1242
NciI CCSGG 1 cut(s) 968
NdeI CATATG 1 cut(s) 521
NlaIII CATG 8 cut(s) 154, 187, 346, 355, 503, 892, 910, 1377
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 809
NsbI TGCGCA 1 cut(s) 412
NspI RCATGY 1 cut(s) 154
NspV TTCGAA 1 cut(s) 1151
PaeR7I CTCGAG 1 cut(s) 489
PagI TCATGA 2 cut(s) 342, 888
PceI AGGCCT 1 cut(s) 207
PciI ACATGT 1 cut(s) 150
PctI GAATGC 1 cut(s) 916
PfeI GAWTC 3 cut(s) 701, 795, 1369
PinAI ACCGGT 1 cut(s) 235
PkrI GCNGC 1 cut(s) 410
Ple19I CGATCG 1 cut(s) 1025
PleI GAGTC 2 cut(s) 500, 1058
PpsI GAGTC 2 cut(s) 500, 1058
Ppu21I YACGTR 1 cut(s) 849
PpuMI RGGWCCY 1 cut(s) 622
PscI ACATGT 1 cut(s) 150
Psp1406I AACGTT 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 622
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 622
PspPPI RGGWCCY 1 cut(s) 622
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 2 cut(s) 1111, 1268
PvuI CGATCG 1 cut(s) 1025
RsaI GTAC 4 cut(s) 58, 240, 507, 847
RsaNI GTAC 4 cut(s) 57, 239, 506, 846
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 7 cut(s) 471, 546, 714, 948, 953, 960, 1316
SatI GCNGC 1 cut(s) 409
Sau96I GGNCC 1 cut(s) 622
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 2 cut(s) 501, 1058
ScrFI CCNGG 1 cut(s) 968
SfcI CTRYAG 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 1151
SinI GGWCC 1 cut(s) 622
SlaI CTCGAG 1 cut(s) 489
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 7 cut(s) 287, 510, 666, 686, 919, 1335, 1345
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 2 cut(s) 927, 1124
SspI AATATT 1 cut(s) 1143
SspMI CTAG 4 cut(s) 117, 324, 626, 1299
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 966
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 5 cut(s) 61, 167, 958, 980, 1079
TaiI ACGT 5 cut(s) 433, 507, 743, 851, 1293
TasI AATT 7 cut(s) 287, 510, 666, 686, 919, 1335, 1345
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 3 cut(s) 701, 795, 1369
Tru1I TTAA 7 cut(s) 471, 546, 714, 948, 953, 960, 1316
Tru9I TTAA 7 cut(s) 471, 546, 714, 948, 953, 960, 1316
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 1 cut(s) 408
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 7 cut(s) 172, 294, 430, 508, 877, 923, 948
TspGWI ACGGA 3 cut(s) 840, 1260, 1278
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 2 cut(s) 666, 686
XbaI TCTAGA 1 cut(s) 116
XceI RCATGY 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XhoI CTCGAG 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 1307
XspI CTAG 4 cut(s) 117, 324, 626, 1299
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.