Rw7G035160

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006952
Physical Location & Seq
Reverse (-)
52411800 .. 52413140
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035160.1

Sequence Viewer

Length: 1341 bp
ATGAATAACACTATTGATGATCTCCCTGATGTTGTATTGGTTGACATTCTCTGTCGACTTCCGTGCTTTGATTTTGTGTTCAAATGCAAGTCTGTGTGCAAACGTTGGAGCATTCTTATGTCGGACCCATACTTCATTGGCCGCTTTCTACTTCGACTCCAAAGCCGACATACGCCAATTCCACCACTTACTCTGATCAATCAAAAGGGGGAGAACTTCCCTATGGCATTCAATCCTCTTTCACCACTCAAGTTTCTAACTAGGATGTTCTTGAGACTCATGAGTTCCCTCTCTTTGAAAAAAGAGCCAGTTGTAGTAGGGGCGTGTAATGACTTGGTTTTGTGTTTCGCAAGTGAGTATTTTCAACGTGATTACTTCATCTGTAATCCATATACCACCCAATGGGTTGCTCTTCCTCCTGTCTCTCGAGTTCTCCGTGACGTACCAGTTGGATTCATATGTGATCCCTACTATAATTATCATAAGAAAGAAGAGGAAGTGCATCAAACAAAAAGTACTATCATACAAGATAAGTTGATTAATATTAACGTGGACTATAGGTGCATGGTAGTGAGAATTCTTCCTTATGATGACACATTTGAAGATGAACTTTCCTACAAATTCAAGGTGGAGATCTTTTCTTTCGAGATTGGTGAATGGAGAGAGTCAATTATCTCATTCCCACAAAGATTTAAACTTGATGATATCGATTCCAATGTTCACTTTGCTTACAATGAAATGCTGTATTGGTCGAGTATGTGGGGAGATTATAACTTTCTGATTGGGTTGAACCCGTTCATGATCAGCGACGTCACTAATAACAACATCATCAGGACCAGTAGTACTAGCAATAGTAACTCCGGTGATAATCCTCAATATTACCCTAAATGTATTGAACTCATAGTTAAAGCCGCTAATTATGATGAGTTATCTTACCTCCATAACTGTCAAATACATGACGTAGTTGTGCACGGAACCGGAGGGTATCTGCAAATATGTCTTTTAGACGTTGAACATTCTGCATTCATTTGGGAGTTAAAAAAAGAAGAAGATGATCTGATAATTGCGGCCGATGGAGCAGGAAAATTGTATTCGAAGCATCTTTTACTGCCTTTGGCCAAAGAAATGGAGCTGATTGGTTTAGACCCATATAATGAAGGCTGCTTATACGTAAGACTCAACGGATCCCTTTTTGATCTTATAAACCTTCGTAGAAGCTTGTTATACGAGATGAACATGCCTAAAAGGAGTCGAAATATTAGTAACTATTGCTTCTTCCCATTAAAAGCGAAACCATGGTGGCCAACACCAGTTCCCAGACTACCTCACCCATCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

446

Amino Acids

51.92

Weight (kDa)

5.85

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 45 7.3e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 771, 1202
AatII GACGTC 1 cut(s) 813
AccB7I CCANNNNNTGG 1 cut(s) 402
AccI GTMKAC 1 cut(s) 55
AciI CCGC 3 cut(s) 142, 912, 1067
AclI AACGTT 1 cut(s) 103
AclWI GGATC 3 cut(s) 458, 1179, 1192
AcoI YGGCCR 4 cut(s) 139, 1068, 1116, 1301
AcsI RAATTY 2 cut(s) 576, 620
AcyI GRCGYC 1 cut(s) 810
AfaI GTAC 3 cut(s) 444, 517, 844
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 9 cut(s) 82, 232, 298, 365, 602, 625, 790, 896, 1013
AluBI AGCT 3 cut(s) 1132, 1218, 1337
AluI AGCT 3 cut(s) 1132, 1218, 1337
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 2 cut(s) 268, 427
Alw44I GTGCAC 1 cut(s) 968
AlwI GGATC 3 cut(s) 458, 1179, 1192
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 4 cut(s) 139, 1068, 1116, 1301
ApaLI GTGCAC 1 cut(s) 968
ApeKI GCWGC 1 cut(s) 1161
ApoI RAATTY 2 cut(s) 576, 620
AseI ATTAAT 1 cut(s) 540
Asp700I GAANNNNTTC 1 cut(s) 794
AspS9I GGNCC 2 cut(s) 124, 834
AsuHPI GGTGA 4 cut(s) 234, 665, 875, 1319
AsuII TTCGAA 1 cut(s) 1094
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 2 cut(s) 124, 834
BaeGI GKGCMC 1 cut(s) 972
BalI TGGCCA 2 cut(s) 1118, 1303
BamHI GGATCC 1 cut(s) 1184
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 1 cut(s) 1148
BccI CCATC 2 cut(s) 1067, 1339
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BclI TGATCA 2 cut(s) 195, 801
BcoDI GTCTC 2 cut(s) 268, 427
BfaI CTAG 2 cut(s) 261, 846
BfmI CTRYAG 1 cut(s) 556
BglII AGATCT 1 cut(s) 633
BisI GCNGC 4 cut(s) 142, 912, 1068, 1162
BlsI GCNGC 4 cut(s) 143, 913, 1069, 1163
BmcAI AGTACT 2 cut(s) 517, 844
Bme18I GGWCC 2 cut(s) 124, 834
BmeT110I CYCGRG 1 cut(s) 426
BmgT120I GGNCC 2 cut(s) 124, 834
BmiI GGNNCC 3 cut(s) 126, 976, 1186
BmsI GCATC 2 cut(s) 511, 1108
Bpu14I TTCGAA 1 cut(s) 1094
BpuEI CTTGAG 2 cut(s) 233, 292
Bsa29I ATCGAT 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 1171
BsaHI GRCGYC 1 cut(s) 810
BsaJI CCNNGG 1 cut(s) 1295
BsaWI WCCGGW 2 cut(s) 860, 977
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 4 cut(s) 308, 446, 837, 1310
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 1 cut(s) 1295
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 402
BseNI ACTGG 4 cut(s) 308, 446, 837, 1310
BseSI GKGCMC 1 cut(s) 972
BseX3I CGGCCG 1 cut(s) 1068
BseXI GCAGC 1 cut(s) 1148
Bsh1285I CGRYCG 1 cut(s) 1071
BshFI GGCC 4 cut(s) 141, 1070, 1118, 1303
BshVI ATCGAT 1 cut(s) 708
BsiEI CGRYCG 1 cut(s) 1071
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 2 cut(s) 861, 978
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 2 cut(s) 268, 427
BsmI GAATGC 3 cut(s) 111, 227, 1022
BsnI GGCC 4 cut(s) 141, 1070, 1118, 1303
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 1094
Bsp1286I GDGCHC 1 cut(s) 972
Bsp143I GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
Bsp19I CCATGG 1 cut(s) 1295
BspACI CCGC 3 cut(s) 142, 912, 1067
BspANI GGCC 4 cut(s) 141, 1070, 1118, 1303
BspDI ATCGAT 1 cut(s) 708
BspHI TCATGA 2 cut(s) 279, 798
BspLI GGNNCC 3 cut(s) 126, 976, 1186
BspPI GGATC 3 cut(s) 458, 1179, 1192
BspQI GCTCTTC 1 cut(s) 417
BspT104I TTCGAA 1 cut(s) 1094
BsrI ACTGG 4 cut(s) 308, 446, 837, 1310
BssECI CCNNGG 1 cut(s) 1295
BssMI GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
BssNI GRCGYC 1 cut(s) 810
BssT1I CCWWGG 1 cut(s) 1295
Bst4CI ACNGT 1 cut(s) 947
Bst6I CTCTTC 2 cut(s) 417, 486
BstACI GRCGYC 1 cut(s) 810
BstBAI YACGTR 1 cut(s) 1171
BstBI TTCGAA 1 cut(s) 1094
BstDSI CCRYGG 1 cut(s) 1295
BstF5I GGATG 1 cut(s) 270
BstKTI GATC 8 cut(s) 22, 198, 466, 636, 804, 1057, 1187, 1198
BstMAI GTCTC 2 cut(s) 268, 427
BstMBI GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
BstMCI CGRYCG 1 cut(s) 1071
BstMWI GCNNNNNNNGC 1 cut(s) 1076
BstNSI RCATGY 1 cut(s) 1240
BstSFI CTRYAG 1 cut(s) 556
BstSLI GKGCMC 1 cut(s) 972
BstSNI TACGTA 1 cut(s) 1171
BstV1I GCAGC 1 cut(s) 1148
BstX2I RGATCY 2 cut(s) 633, 1184
BstXI CCANNNNNNTGG 1 cut(s) 1126
BstYI RGATCY 2 cut(s) 633, 1184
BstZI CGGCCG 1 cut(s) 1068
Bsu15I ATCGAT 1 cut(s) 708
BsuRI GGCC 4 cut(s) 141, 1070, 1118, 1303
BsuTUI ATCGAT 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 1295
BtsCI GGATG 1 cut(s) 270
CciI TCATGA 2 cut(s) 279, 798
Cfr13I GGNCC 2 cut(s) 124, 834
ClaI ATCGAT 1 cut(s) 708
Csp6I GTAC 3 cut(s) 443, 516, 843
CviAII CATG 6 cut(s) 280, 565, 799, 956, 1237, 1296
CviQI GTAC 3 cut(s) 443, 516, 843
DpnI GATC 8 cut(s) 21, 197, 465, 635, 803, 1056, 1186, 1197
DpnII GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 4 cut(s) 139, 1068, 1116, 1301
EagI CGGCCG 1 cut(s) 1068
Eam1104I CTCTTC 2 cut(s) 417, 486
EarI CTCTTC 2 cut(s) 417, 486
EclXI CGGCCG 1 cut(s) 1068
Eco105I TACGTA 1 cut(s) 1171
Eco130I CCWWGG 1 cut(s) 1295
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 2 cut(s) 124, 834
Eco52I CGGCCG 1 cut(s) 1068
Eco88I CYCGRG 1 cut(s) 426
EcoRI GAATTC 1 cut(s) 576
EcoRV GATATC 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 1295
ErhI CCWWGG 1 cut(s) 1295
FaeI CATG 6 cut(s) 283, 568, 802, 959, 1240, 1299
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FatI CATG 6 cut(s) 279, 564, 798, 955, 1236, 1295
FauNDI CATATG 1 cut(s) 458
FbaI TGATCA 2 cut(s) 195, 801
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 4 cut(s) 142, 912, 1068, 1162
FokI GGATG 1 cut(s) 277
Fsp4HI GCNGC 4 cut(s) 142, 912, 1068, 1162
FspBI CTAG 2 cut(s) 261, 846
GluI GCNGC 4 cut(s) 142, 912, 1068, 1162
HaeIII GGCC 4 cut(s) 141, 1070, 1118, 1303
HapII CCGG 2 cut(s) 861, 978
Hin1I GRCGYC 1 cut(s) 810
Hin1II CATG 6 cut(s) 283, 568, 802, 959, 1240, 1299
HincII GTYRAC 2 cut(s) 43, 56
HindII GTYRAC 2 cut(s) 43, 56
HindIII AAGCTT 1 cut(s) 1216
HinfI GANTC 7 cut(s) 156, 276, 453, 665, 710, 1176, 1249
HpaII CCGG 2 cut(s) 861, 978
HphI GGTGA 4 cut(s) 234, 665, 875, 1319
Hpy166II GTNNAC 5 cut(s) 43, 56, 553, 721, 970
Hpy188I TCNGA 4 cut(s) 124, 195, 780, 1059
Hpy188III TCNNGA 6 cut(s) 271, 280, 426, 646, 799, 832
Hpy8I GTNNAC 5 cut(s) 43, 56, 553, 721, 970
Hpy99I CGWCG 1 cut(s) 812
HpyAV CCTTC 2 cut(s) 1151, 1217
HpyCH4III ACNGT 1 cut(s) 947
HpyCH4IV ACGT 8 cut(s) 103, 367, 441, 549, 810, 960, 1008, 1170
HpyCH4V TGCA 7 cut(s) 87, 99, 502, 564, 970, 991, 1022
HpyF10VI GCNNNNNNNGC 1 cut(s) 1076
HpySE526I ACGT 8 cut(s) 103, 367, 441, 549, 810, 960, 1008, 1170
Hsp92I GRCGYC 1 cut(s) 810
Hsp92II CATG 6 cut(s) 283, 568, 802, 959, 1240, 1299
Ksp22I TGATCA 2 cut(s) 195, 801
Kzo9I GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 3 cut(s) 108, 1076, 1129
Lsp1109I GCAGC 1 cut(s) 1148
LweI GCATC 2 cut(s) 511, 1108
MaeI CTAG 2 cut(s) 261, 846
MaeII ACGT 8 cut(s) 103, 367, 441, 549, 810, 960, 1008, 1170
MaeIII GTNAC 4 cut(s) 437, 811, 854, 1262
MalI GATC 8 cut(s) 21, 197, 465, 635, 803, 1056, 1186, 1197
MboI GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
MboII GAAGA 7 cut(s) 404, 503, 572, 614, 1058, 1061, 1267
MflI RGATCY 2 cut(s) 633, 1184
MhlI GDGCHC 1 cut(s) 972
MlsI TGGCCA 2 cut(s) 1118, 1303
MluCI AATT 8 cut(s) 177, 475, 576, 620, 669, 916, 1062, 1085
MluNI TGGCCA 2 cut(s) 1118, 1303
MlyI GAGTC 5 cut(s) 150, 270, 674, 1170, 1258
MmeI TCCRAC 3 cut(s) 86, 102, 430
MnlI CCTC 8 cut(s) 246, 299, 426, 487, 882, 947, 974, 1335
Mox20I TGGCCA 2 cut(s) 1118, 1303
MroXI GAANNNNTTC 1 cut(s) 794
MscI TGGCCA 2 cut(s) 1118, 1303
MseI TTAA 6 cut(s) 540, 546, 693, 906, 1037, 1283
MslI CAYNNNNRTG 2 cut(s) 116, 569
Msp20I TGGCCA 2 cut(s) 1118, 1303
MspI CCGG 2 cut(s) 861, 978
Mva1269I GAATGC 3 cut(s) 111, 227, 1022
MwoI GCNNNNNNNGC 1 cut(s) 1076
NcoI CCATGG 1 cut(s) 1295
NdeI CATATG 1 cut(s) 458
NdeII GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
NlaIII CATG 6 cut(s) 283, 568, 802, 959, 1240, 1299
NlaIV GGNNCC 3 cut(s) 126, 976, 1186
NmuCI GTSAC 2 cut(s) 437, 811
NspI RCATGY 1 cut(s) 1240
NspV TTCGAA 1 cut(s) 1094
PaeR7I CTCGAG 1 cut(s) 426
PagI TCATGA 2 cut(s) 279, 798
PciSI GCTCTTC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PctI GAATGC 3 cut(s) 111, 227, 1022
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 2 cut(s) 453, 710
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 4 cut(s) 143, 913, 1069, 1163
PleI GAGTC 5 cut(s) 150, 270, 673, 1170, 1257
PpsI GAGTC 5 cut(s) 150, 270, 673, 1170, 1257
Ppu21I YACGTR 1 cut(s) 1171
PshBI ATTAAT 1 cut(s) 540
PsiI TTATAA 2 cut(s) 771, 1202
Psp1406I AACGTT 1 cut(s) 103
PspN4I GGNNCC 3 cut(s) 126, 976, 1186
PspPI GGNCC 2 cut(s) 124, 834
PsuI RGATCY 2 cut(s) 633, 1184
RsaI GTAC 3 cut(s) 444, 517, 844
RsaNI GTAC 3 cut(s) 443, 516, 843
RseI CAYNNNNRTG 2 cut(s) 116, 569
SalI GTCGAC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 6 cut(s) 540, 546, 693, 906, 1037, 1283
SatI GCNGC 4 cut(s) 142, 912, 1068, 1162
Sau3AI GATC 8 cut(s) 19, 195, 463, 633, 801, 1054, 1184, 1195
Sau96I GGNCC 2 cut(s) 124, 834
ScaI AGTACT 2 cut(s) 517, 844
SchI GAGTC 5 cut(s) 150, 270, 674, 1170, 1258
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 2 cut(s) 511, 1108
SfcI CTRYAG 1 cut(s) 556
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 1094
SinI GGWCC 2 cut(s) 124, 834
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 116, 569
SmlI CTYRAG 3 cut(s) 248, 271, 426
SmoI CTYRAG 3 cut(s) 248, 271, 426
SnaBI TACGTA 1 cut(s) 1171
Sse9I AATT 8 cut(s) 177, 475, 576, 620, 669, 916, 1062, 1085
SsiI CCGC 3 cut(s) 142, 912, 1067
SspI AATATT 3 cut(s) 544, 878, 1258
SspMI CTAG 2 cut(s) 261, 846
StyI CCWWGG 1 cut(s) 1295
TaaI ACNGT 1 cut(s) 947
TaiI ACGT 8 cut(s) 106, 370, 444, 552, 813, 963, 1011, 1173
TaqI TCGA 8 cut(s) 55, 154, 427, 645, 708, 752, 1094, 1252
TasI AATT 8 cut(s) 177, 475, 576, 620, 669, 916, 1062, 1085
TatI WGTACW 2 cut(s) 515, 842
TauI GCSGC 3 cut(s) 144, 914, 1070
TfiI GAWTC 2 cut(s) 453, 710
Tru1I TTAA 6 cut(s) 540, 546, 693, 906, 1037, 1283
Tru9I TTAA 6 cut(s) 540, 546, 693, 906, 1037, 1283
TseFI GTSAC 2 cut(s) 437, 811
TseI GCWGC 1 cut(s) 1161
Tsp45I GTSAC 2 cut(s) 437, 811
TspGWI ACGGA 4 cut(s) 51, 425, 987, 1197
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 968
VpaK11BI GGWCC 2 cut(s) 124, 834
VspI ATTAAT 1 cut(s) 540
XapI RAATTY 2 cut(s) 576, 620
XceI RCATGY 1 cut(s) 1240
XhoI CTCGAG 1 cut(s) 426
XmiI GTMKAC 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 794
XspI CTAG 2 cut(s) 261, 846
ZraI GACGTC 1 cut(s) 811
ZrmI AGTACT 2 cut(s) 517, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.