Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002708

Physical Location & Seq

Genomic Coordinates Reverse (-)

12968724 .. 12973559

Gene Structure 4836 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1067300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

ATGCCTTCCGGCGCCGACGACGGCGCCCAGTACTCGTTTGCGATAGAGTACAAGGGTCCTGCGGTCAGTTACGATCTTCCTCGGGCGGTTCCTATCAACGTCGACAGAATTCCAGTGGCGGCGGTGGTCGGAGGGGTTGCGCTGCCCGAGAAGCTCACCTTGCCGGTGGTCCAGCCGGTGCTTGCGCCCGCAACGTTCTCTAAAGAGCTCAAGGCGTCGAACTCGACGGTGTCTCCGACTTCCGTTTTTGATCGGGGCAGCGAGGATTACACGAAGGAGTTGGAAGGTTCTGAATCGACTGTTTCACGGACGTCGGTGATTGGATTTGAGGAGACTGGTGTGGAGAGTGTGGCTGGGTGTGCGTTGTCCGGTGAATTGAGCAGCTCCGGCCCGTCGGAGTTTTCGAACGGGTTGAATAACAGGTCGGGTGAGTTTTCCGATGTGATTGGTGACGGGAAGGAGAGTGTGGACTTCAACAGTGATTCGAATCGGCCAGAACCTGAGCTGGACTGGGTCTCCACGGAGTCGGTTTTGAGCTTGGACTATCCGTCGTCTCGTGTTTCATCCAGTAAGGCTTTGGATTATGATGCACGGCGGCCGCCGGTGGTGACTTTTCGGGACATTGAGTCGGATGATGGGGCTGATGAAGACGAGGCTGAGGTTGTGCAGGCGAAGCGGGAGCCTCAGAGCAAAGGGAAGAAGAAGGCGTGCTACCGATGTTTGAAGGGGACGAGGTTTACTGAGAAGGAGGTTTGCATTGTTTGTGATGCCAAGTATTGTAGTAGTTGTGTGCTTAGAGCAATGGGATCAATGCCCGAAGGTCGAAAATGTGTGGCTTGTATTGGGTTTCCAATTGAGGAGTCGAAAAGAGGGAGCTTGGGGAAGTGCTCTAGAATGCTCAAGCGGCTGTTGAATGATTTGGAGGTTCGACAGGTGATGAAATCCGAAAAATTTTGTGAGGCAAATCAGCTGCCCCCCGATTATATTTGTGTTAATGGACAGCCTCTTTGTCATGAGGAGCTGGTTCTATTACAAACCTGCCCTAATCCGCCGAAGAAATTAAAACCAGGAAACTATTGGTATGATAAAGTTTCTGGTCTTTGGGGAAAGGAAGGGCAGAAGCCTTCTAAGATCATTAGTCCCCATCTCAGTGTTGGGGGTCCTATCAAGGCAAATGCTAGCAATGGGAACACACAGGTTTACATAAATGGCCGGGAAATTACCAAAGTTGAGCTTCGGATGTTGCAGTTGGCTGGGGTTCAATGTGCTGGTAACCCACATTTTTGGGTGAACGAAGATGGGTCATACCAAGAGGAGGGCCAGAAAAATACTAAAGGATACATATGGGGCAAGGCTGGAACAAAGCTTGTCTGTGCTGCCTTGTCACTACCAGTCCCTTCTAAATCTTCAACTCCTTGTGGAAAATCCTTCAACTATGTGGGTAGTAGGGTTGTCCCTGATTACATTGAGCGGAGAATTCTTCAGAAGATTCTTTTAGTTGGATGTAATGGTTCTGGAACAAGTACTATATTTAAACAGGCCAAGATTCTATATAAAGCTGTCCCTTTCTTGGAGGATGAGCGTGAAAATATCAAATGTAAAATACAGAGTAATGTGTATGGTTATCTTGGTATACTTCTTGAGGGCCGTGAGCGTTTTGAAGAGGAATGTTTGGATGGGATGAGGAAGAAATGTTCTTCCAGTCAAACAGATGCCAATGGGAACAATGACAAAACTCTTTACTCCGTTGGGCCGAGGCTGAAAGCGTTCTCTGATTGGCTTCTCAAGACTATGGTGTCAGGCAATCTGGAAGCCATTTTTCCAGCTGCTACTCGTGAATATGCACCATTAGTTGAGGAATTGTGGAATGATTCAGCCATTCAGGCCACATACAAGCGGAGAAGGGAATTGGAAATGCTACCTAGTGTTGCGACTTATTTCCTAGAGAGGGCTGTTGACATATTAAGAATAGACTACGAGCCCACAGATCTAGACATCCTATATGCTGAAGGTGTCACTTCATCTAATGGGCTTGCTTGTGTGGACTTCTCATTTCCCAACTCAGAATCTGAAGATACCATCAATACTAGTGATCAGCATGACTCTTTGCTTAGGTACCAACTTATTAGAGTAAATGCAAGAGGACTTGGAGAAAACTGTAAATGGTTAGAGATGTTTGAAGATGTTGGAATGGTCATATTCTGCGTGTCCTTGAGCGACTACGATCGGTTCTCTGCTGATGGGAATGGGTATTTCAATAACAAGATGTTGCTGGCCAGGACTTTCTTTGAAAGCATTGTTACTCATCCGACCTTCGAACAGATGGACTTCCTCTTGATTCTAAACAAGTTTGATTTATTTGAGGATAAGGTAGAACGGGTACCGTTAAATCAGTGTGAGTGGTTTGAAGATTTTCGTCCAGTGATGAGTCGTCATCGCTCGAGTAGCAACACGAGCAGCAACAACATTAACAGCAGCCCCTCATTGGGTCATTTAGGAGCTCACTACATTGCAGCAAAGTTCAAGAGGCTCTATTCTTCTCTAACCGGGAAGAAATTGTACGTTTCCGTGGTGAAGGGATTGCAGCCGGATAGTGTCGATGCAGCTCTTAAATATTCGAGGGAGATTTTAAAGTGGGATGAAGAGAGGGCCAATTTTGGCTTTGATTATTCGGTCTATAGCACCGAAGCAAGCTCCTATTCAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009898 GO:0009987 GO:0016020 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0030234 GO:0030695 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0034260 GO:0035639 GO:0036094 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0048519 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051346 GO:0051716 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

903

Amino Acids

100.07

Weight (kDa)

5.39

Isoelectric Point (pI)

46.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	495 - 874	3.9e-63	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23460
fragaria_vesca	FvH4_1g14590 FvH4_1g14590
malus_domestica	MD00G1067300.v1.1 MD02G1159500.v1.1
prunus_persica	Prupe.7G144400_v2.0.a1
pyrus_communis	pycom02g12580 pycom15g23720
rosa_chinensis	RchiOBHm_Chr1g0367271 RchiOBHm_Chr1g0367281 RchiOBHm_Chr1g0367331 RchiOBHm_Chr2g0103371
rosa_laevigata	RLG00000017231 RLG00000027279 RLG00000027284
rosa_multiflora	Rmu_sc0001555.1_g000003 Rmu_sc0002434.1_g000010 Rmu_sc0011000.1_g000013
rosa_roxburghii	Rroxscaffold_2G00139680 Rroxscaffold_4G00289390 Rroxscaffold_4G00289470 Rroxscaffold_4G00289480
rosa_rugosa	Rorug02G0114700 Rorug06G0002300
rosa_samantha	Rh1AG342600 Rh1BG303200 Rh1BG303600 Rh1BG303700 Rh1CG319500 Rh1CG319700 Rh1DG335600 Rh1DG336000 Rh1DG336100 Rh2AG162700 Rh2BG169800 Rh2CG169900 Rh2DG168800
rosa_wichuraiana	Rw1G030440 Rw2G012780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1795
AatII	GACGTC	1 cut(s)	314
Acc36I	ACCTGC	1 cut(s)	1046
Acc65I	GGTACC	2 cut(s)	2115, 2380
AccB1I	GGYRCC	4 cut(s)	11, 23, 2115, 2380
AccBSI	CCGCTC	1 cut(s)	1471
AccI	GTMKAC	2 cut(s)	102, 1633
AclI	AACGTT	1 cut(s)	194
AclWI	GGATC	1 cut(s)	814
AcoI	YGGCCR	4 cut(s)	491, 596, 1210, 2274
AcsI	RAATTY	3 cut(s)	108, 950, 1476
AcuI	CTGAAG	3 cut(s)	1466, 2028, 2091
AcyI	GRCGYC	4 cut(s)	12, 24, 215, 311
AfaI	GTAC	6 cut(s)	32, 50, 1525, 2117, 2382, 2561
AfiI	CCNNNNNNNGG	5 cut(s)	1315, 1570, 1948, 2485, 2486
AhdI	GACNNNNNGTC	2 cut(s)	547, 625
AhlI	ACTAGT	1 cut(s)	2087
AjnI	CCWGG	2 cut(s)	1066, 2276
AloI	GAACNNNNNNTCC	2 cut(s)	1678, 1710
Alw21I	GWGCWC	3 cut(s)	210, 890, 2503
Alw26I	GTCTC	4 cut(s)	237, 326, 520, 558
AlwI	GGATC	1 cut(s)	814
AlwNI	CAGNNNCTG	2 cut(s)	500, 2069
Ama87I	CYCGRG	3 cut(s)	81, 146, 2440
ApoI	RAATTY	3 cut(s)	108, 950, 1476
ArsI	GACNNNNNNTTYG	2 cut(s)	990, 1022
Asp700I	GAANNNNTTC	2 cut(s)	1766, 2412
Asp718I	GGTACC	2 cut(s)	2115, 2380
AspLEI	GCGC	4 cut(s)	14, 26, 142, 187
AspS9I	GGNCC	8 cut(s)	56, 169, 389, 1160, 1318, 1645, 1751, 2649
AsuC2I	CCSGG	2 cut(s)	1214, 2548
AsuHPI	GGTGA	9 cut(s)	148, 328, 383, 440, 461, 619, 946, 1300, 2584
AsuII	TTCGAA	3 cut(s)	404, 485, 2316
AsuNHI	GCTAGC	1 cut(s)	1178
AvaI	CYCGRG	3 cut(s)	81, 146, 2440
AvaII	GGWCC	3 cut(s)	56, 169, 1160
BalI	TGGCCA	1 cut(s)	2276
BanI	GGYRCC	4 cut(s)	11, 23, 2115, 2380
BanII	GRGCYC	3 cut(s)	210, 1983, 2503
BarI	GAAGNNNNNNTAC	4 cut(s)	695, 727, 2543, 2575
BauI	CACGAG	3 cut(s)	555, 1833, 2452
BbsI	GAAGAC	1 cut(s)	654
Bbv12I	GWGCWC	3 cut(s)	210, 890, 2503
BbvCI	CCTCAGC	1 cut(s)	657
BccI	CCATC	7 cut(s)	629, 1152, 1292, 1670, 2087, 2234, 2317
BceAI	ACGGC	3 cut(s)	37, 608, 1632
BciT130I	CCWGG	2 cut(s)	1068, 2278
BciVI	GTATCC	1 cut(s)	1331
BclI	TGATCA	1 cut(s)	2092
BcnI	CCSGG	2 cut(s)	1214, 2548
BcoDI	GTCTC	4 cut(s)	237, 326, 520, 558
BcuI	ACTAGT	1 cut(s)	2087
BfaI	CTAG	6 cut(s)	891, 1179, 1923, 1943, 1991, 2088
BfmI	CTRYAG	1 cut(s)	2677
BfoI	RGCGCY	2 cut(s)	15, 27
BfuAI	ACCTGC	1 cut(s)	1046
BfuI	GTATCC	1 cut(s)	1331
BglI	GCCNNNNNGGC	1 cut(s)	1883
BglII	AGATCT	1 cut(s)	1987
BmcAI	AGTACT	2 cut(s)	32, 1525
Bme1390I	CCNGG	4 cut(s)	1068, 1214, 2278, 2548
Bme18I	GGWCC	3 cut(s)	56, 169, 1160
BmeRI	GACNNNNNGTC	2 cut(s)	547, 625
BmeT110I	CYCGRG	3 cut(s)	81, 146, 2440
BmgT120I	GGNCC	8 cut(s)	56, 169, 389, 1160, 1318, 1645, 1751, 2649
BmiI	GGNNCC	8 cut(s)	13, 25, 57, 90, 681, 1161, 2117, 2382
BmrFI	CCNGG	4 cut(s)	1068, 1214, 2278, 2548
BmrI	ACTGGG	2 cut(s)	22, 520
BmsI	GCATC	4 cut(s)	577, 757, 1702, 2590
BmtI	GCTAGC	1 cut(s)	1182
BmuI	ACTGGG	2 cut(s)	22, 520
BpiI	GAAGAC	1 cut(s)	654
Bpu10I	CCTNAGC	3 cut(s)	501, 657, 2111
Bpu14I	TTCGAA	3 cut(s)	404, 485, 2316
BpuEI	CTTGAG	5 cut(s)	194, 884, 1661, 1769, 2233
BpuMI	CCSGG	2 cut(s)	1214, 2548
BsaBI	GATNNNNATC	1 cut(s)	486
BsaHI	GRCGYC	4 cut(s)	12, 24, 215, 311
BsaI	GGTCTC	1 cut(s)	520
BsaJI	CCNNGG	4 cut(s)	80, 519, 1754, 2568
BsaWI	WCCGGW	1 cut(s)	368
BsaXI	ACNNNNNCTCC	4 cut(s)	217, 247, 500, 530
Bsc4I	CCNNNNNNNGG	5 cut(s)	1315, 1570, 1948, 2485, 2486
Bse118I	RCCGGY	3 cut(s)	163, 175, 601
Bse1I	ACTGG	8 cut(s)	28, 113, 340, 515, 567, 1391, 1701, 2420
Bse3DI	GCAATG	3 cut(s)	807, 1189, 2508
Bse8I	GATNNNNATC	1 cut(s)	486
BseBI	CCWGG	2 cut(s)	1068, 2278
BseDI	CCNNGG	4 cut(s)	80, 519, 1754, 2568
BseJI	GATNNNNATC	1 cut(s)	486
BseLI	CCNNNNNNNGG	5 cut(s)	1315, 1570, 1948, 2485, 2486
BseMI	GCAATG	3 cut(s)	807, 1189, 2508
BseMII	CTCAG	6 cut(s)	492, 648, 698, 732, 1162, 2076
BseNI	ACTGG	8 cut(s)	28, 113, 340, 515, 567, 1391, 1701, 2420
BseRI	GAGGAG	4 cut(s)	344, 872, 1031, 1328
BseX3I	CGGCCG	1 cut(s)	596
BseYI	CCCAGC	2 cut(s)	353, 1253
BsgI	GTGCAG	1 cut(s)	686
Bsh1285I	CGRYCG	2 cut(s)	599, 2227
BshNI	GGYRCC	4 cut(s)	11, 23, 2115, 2380
BsiEI	CGRYCG	2 cut(s)	599, 2227
BsiHKAI	GWGCWC	3 cut(s)	210, 890, 2503
BsiHKCI	CYCGRG	3 cut(s)	81, 146, 2440
BsiSI	CCGG	9 cut(s)	9, 164, 176, 369, 387, 602, 1213, 2547, 2588
BslFI	GGGAC	6 cut(s)	632, 742, 1125, 1379, 1439, 1547
BslI	CCNNNNNNNGG	5 cut(s)	1315, 1570, 1948, 2485, 2486
BsmAI	GTCTC	4 cut(s)	237, 326, 520, 558
BsmBI	CGTCTC	1 cut(s)	558
BsmFI	GGGAC	6 cut(s)	632, 742, 1125, 1379, 1439, 1547
BsmI	GAATGC	1 cut(s)	900
Bso31I	GGTCTC	1 cut(s)	520
BsoBI	CYCGRG	3 cut(s)	81, 146, 2440
Bsp119I	TTCGAA	3 cut(s)	404, 485, 2316
Bsp1286I	GDGCHC	4 cut(s)	210, 890, 1983, 2503
Bsp143I	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
BspCNI	CTCAG	6 cut(s)	493, 649, 697, 733, 1161, 2075
BspHI	TCATGA	1 cut(s)	1012
BspLI	GGNNCC	8 cut(s)	13, 25, 57, 90, 681, 1161, 2117, 2382
BspMI	ACCTGC	1 cut(s)	1046
BspOI	GCTAGC	1 cut(s)	1182
BspPI	GGATC	1 cut(s)	814
BspT104I	TTCGAA	3 cut(s)	404, 485, 2316
BspT107I	GGYRCC	4 cut(s)	11, 23, 2115, 2380
BspTNI	GGTCTC	1 cut(s)	520
BsrBI	CCGCTC	1 cut(s)	1471
BsrDI	GCAATG	3 cut(s)	807, 1189, 2508
BsrFI	RCCGGY	3 cut(s)	163, 175, 601
BsrI	ACTGG	8 cut(s)	28, 113, 340, 515, 567, 1391, 1701, 2420
BssAI	RCCGGY	3 cut(s)	163, 175, 601
BssECI	CCNNGG	4 cut(s)	80, 519, 1754, 2568
BssMI	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
BssNAI	GTATAC	1 cut(s)	1634
BssNI	GRCGYC	4 cut(s)	12, 24, 215, 311
BssSI	CACGAG	3 cut(s)	555, 1833, 2452
Bst1107I	GTATAC	1 cut(s)	1634
Bst2BI	CACGAG	3 cut(s)	555, 1833, 2452
Bst2UI	CCWGG	2 cut(s)	1068, 2278
Bst4CI	ACNGT	5 cut(s)	229, 301, 479, 2159, 2385
Bst6I	CTCTTC	2 cut(s)	1656, 2637
BstACI	GRCGYC	4 cut(s)	12, 24, 215, 311
BstBI	TTCGAA	3 cut(s)	404, 485, 2316
BstC8I	GCNNGC	8 cut(s)	183, 189, 669, 709, 1180, 2034, 2274, 2692
BstDEI	CTNAG	9 cut(s)	501, 657, 684, 741, 794, 1128, 1148, 2062, 2111
BstDSI	CCRYGG	2 cut(s)	519, 2568
BstEII	GGTNACC	1 cut(s)	1271
BstH2I	RGCGCY	2 cut(s)	15, 27
BstHHI	GCGC	4 cut(s)	14, 26, 142, 187
BstKTI	GATC	7 cut(s)	76, 253, 809, 1134, 1990, 2095, 2227
BstMAI	GTCTC	4 cut(s)	237, 326, 520, 558
BstMBI	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
BstMCI	CGRYCG	2 cut(s)	599, 2227
BstMWI	GCNNNNNNNGC	9 cut(s)	151, 160, 359, 387, 673, 904, 1883, 2445, 2454
BstNI	CCWGG	2 cut(s)	1068, 2278
BstPI	GGTNACC	1 cut(s)	1271
BstSCI	CCNGG	4 cut(s)	1066, 1212, 2276, 2546
BstSFI	CTRYAG	1 cut(s)	2677
BstV2I	GAAGAC	1 cut(s)	654
BstX2I	RGATCY	1 cut(s)	1987
BstXI	CCANNNNNNTGG	1 cut(s)	1284
BstYI	RGATCY	1 cut(s)	1987
BstZ17I	GTATAC	1 cut(s)	1634
BstZI	CGGCCG	1 cut(s)	596
BsuI	GTATCC	1 cut(s)	1331
BtgI	CCRYGG	2 cut(s)	519, 2568
BtgZI	GCGATG	1 cut(s)	2420
BtsIMutI	CAGTG	5 cut(s)	120, 484, 1156, 2399, 2427
BveI	ACCTGC	1 cut(s)	1046
Cac8I	GCNNGC	8 cut(s)	183, 189, 669, 709, 1180, 2034, 2274, 2692
CaiI	CAGNNNCTG	2 cut(s)	500, 2069
CciI	TCATGA	1 cut(s)	1012
CciNI	GCGGCCGC	1 cut(s)	596
CfoI	GCGC	4 cut(s)	14, 26, 142, 187
Cfr10I	RCCGGY	3 cut(s)	163, 175, 601
Cfr13I	GGNCC	8 cut(s)	56, 169, 389, 1160, 1318, 1645, 1751, 2649
CseI	GACGC	1 cut(s)	204
Csp6I	GTAC	6 cut(s)	31, 49, 1524, 2116, 2381, 2560
CviAII	CATG	2 cut(s)	1013, 2099
CviQI	GTAC	6 cut(s)	31, 49, 1524, 2116, 2381, 2560
DdeI	CTNAG	9 cut(s)	501, 657, 684, 741, 794, 1128, 1148, 2062, 2111
DinI	GGCGCC	2 cut(s)	13, 25
DpnI	GATC	7 cut(s)	75, 252, 808, 1133, 1989, 2094, 2226
DpnII	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
DraI	TTTAAA	2 cut(s)	1534, 2631
DrdI	GACNNNNNNGTC	1 cut(s)	1795
DriI	GACNNNNNGTC	2 cut(s)	547, 625
DseDI	GACNNNNNNGTC	1 cut(s)	1795
EaeI	YGGCCR	4 cut(s)	491, 596, 1210, 2274
EagI	CGGCCG	1 cut(s)	596
Eam1104I	CTCTTC	2 cut(s)	1656, 2637
Eam1105I	GACNNNNNGTC	2 cut(s)	547, 625
EarI	CTCTTC	2 cut(s)	1656, 2637
EciI	GGCGGA	1 cut(s)	1038
Ecl136II	GAGCTC	2 cut(s)	208, 2501
EclXI	CGGCCG	1 cut(s)	596
Eco24I	GRGCYC	3 cut(s)	210, 1983, 2503
Eco31I	GGTCTC	1 cut(s)	520
Eco47I	GGWCC	3 cut(s)	56, 169, 1160
Eco52I	CGGCCG	1 cut(s)	596
Eco53kI	GAGCTC	2 cut(s)	208, 2501
Eco57I	CTGAAG	3 cut(s)	1466, 2028, 2091
Eco88I	CYCGRG	3 cut(s)	81, 146, 2440
Eco91I	GGTNACC	1 cut(s)	1271
EcoICRI	GAGCTC	2 cut(s)	208, 2501
EcoO109I	RGGNCCY	2 cut(s)	56, 1160
EcoO65I	GGTNACC	1 cut(s)	1271
EcoRI	GAATTC	2 cut(s)	108, 1476
EcoRII	CCWGG	2 cut(s)	1066, 2276
EcoT38I	GRGCYC	3 cut(s)	210, 1983, 2503
EgeI	GGCGCC	2 cut(s)	13, 25
EheI	GGCGCC	2 cut(s)	13, 25
Esp3I	CGTCTC	1 cut(s)	558
FaeI	CATG	2 cut(s)	1016, 2102
FalI	AAGNNNNNCTT	4 cut(s)	143, 175, 1218, 1250
FaqI	GGGAC	6 cut(s)	632, 742, 1125, 1379, 1439, 1547
FatI	CATG	2 cut(s)	1012, 2098
FauI	CCCGC	2 cut(s)	196, 669
FauNDI	CATATG	1 cut(s)	1343
FbaI	TGATCA	1 cut(s)	2092
FblI	GTMKAC	2 cut(s)	102, 1633
FriOI	GRGCYC	3 cut(s)	210, 1983, 2503
FspBI	CTAG	6 cut(s)	891, 1179, 1923, 1943, 1991, 2088
GlaI	GCGC	4 cut(s)	13, 25, 141, 186
GsaI	CCCAGC	2 cut(s)	357, 1257
HaeII	RGCGCY	2 cut(s)	15, 27
HapII	CCGG	9 cut(s)	9, 164, 176, 369, 387, 602, 1213, 2547, 2588
HgaI	GACGC	1 cut(s)	204
HhaI	GCGC	4 cut(s)	14, 26, 142, 187
Hin1I	GRCGYC	4 cut(s)	12, 24, 215, 311
Hin1II	CATG	2 cut(s)	1016, 2102
Hin6I	GCGC	4 cut(s)	12, 24, 140, 185
HinP1I	GCGC	4 cut(s)	12, 24, 140, 185
HincII	GTYRAC	2 cut(s)	103, 1957
HindII	GTYRAC	2 cut(s)	103, 1957
HindIII	AAGCTT	1 cut(s)	1364
HpaII	CCGG	9 cut(s)	9, 164, 176, 369, 387, 602, 1213, 2547, 2588
HphI	GGTGA	9 cut(s)	148, 328, 383, 440, 461, 619, 946, 1300, 2584
Hpy166II	GTNNAC	8 cut(s)	103, 469, 738, 1201, 1291, 1634, 1957, 2044
Hpy8I	GTNNAC	8 cut(s)	103, 469, 738, 1201, 1291, 1634, 1957, 2044
Hpy99I	CGWCG	8 cut(s)	20, 23, 104, 220, 229, 316, 397, 553
HpyCH4III	ACNGT	5 cut(s)	229, 301, 479, 2159, 2385
HpyCH4IV	ACGT	4 cut(s)	99, 194, 311, 2562
HpyCH4V	TGCA	9 cut(s)	590, 667, 756, 1246, 1844, 2138, 2513, 2584, 2603
HpyF10VI	GCNNNNNNNGC	9 cut(s)	151, 160, 359, 387, 673, 904, 1883, 2445, 2454
HpyF3I	CTNAG	9 cut(s)	501, 657, 684, 741, 794, 1128, 1148, 2062, 2111
HpySE526I	ACGT	4 cut(s)	99, 194, 311, 2562
Hsp92I	GRCGYC	4 cut(s)	12, 24, 215, 311
Hsp92II	CATG	2 cut(s)	1016, 2102
HspAI	GCGC	4 cut(s)	12, 24, 140, 185
KasI	GGCGCC	2 cut(s)	11, 23
KpnI	GGTACC	2 cut(s)	2119, 2384
Ksp22I	TGATCA	1 cut(s)	2092
Kzo9I	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
LmnI	GCTCC	6 cut(s)	389, 679, 873, 1018, 2498, 2699
LweI	GCATC	4 cut(s)	577, 757, 1702, 2590
MaeI	CTAG	6 cut(s)	891, 1179, 1923, 1943, 1991, 2088
MaeII	ACGT	4 cut(s)	99, 194, 311, 2562
MaeIII	GTNAC	7 cut(s)	68, 449, 607, 1271, 1383, 2014, 2299
MalI	GATC	7 cut(s)	75, 252, 808, 1133, 1989, 2094, 2226
MbiI	CCGCTC	1 cut(s)	1471
MboI	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
MfeI	CAATTG	1 cut(s)	852
MflI	RGATCY	1 cut(s)	1987
MhlI	GDGCHC	4 cut(s)	210, 890, 1983, 2503
MlsI	TGGCCA	1 cut(s)	2276
MluNI	TGGCCA	1 cut(s)	2276
Mly113I	GGCGCC	2 cut(s)	12, 24
MlyI	GAGTC	5 cut(s)	533, 635, 869, 2096, 2437
MmeI	TCCRAC	8 cut(s)	109, 260, 261, 375, 609, 1480, 2167, 2333
Mox20I	TGGCCA	1 cut(s)	2276
MroXI	GAANNNNTTC	2 cut(s)	1766, 2412
MscI	TGGCCA	1 cut(s)	2276
MseI	TTAA	8 cut(s)	993, 1061, 1533, 1964, 2387, 2469, 2610, 2630
MslI	CAYNNNNRTG	1 cut(s)	1149
Msp20I	TGGCCA	1 cut(s)	2276
MspA1I	CMGCKG	2 cut(s)	970, 1826
MspI	CCGG	9 cut(s)	9, 164, 176, 369, 387, 602, 1213, 2547, 2588
MspR9I	CCNGG	4 cut(s)	1068, 1214, 2278, 2548
MunI	CAATTG	1 cut(s)	852
Mva1269I	GAATGC	1 cut(s)	900
MvaI	CCWGG	2 cut(s)	1068, 2278
MwoI	GCNNNNNNNGC	9 cut(s)	151, 160, 359, 387, 673, 904, 1883, 2445, 2454
NarI	GGCGCC	2 cut(s)	12, 24
NciI	CCSGG	2 cut(s)	1214, 2548
NdeI	CATATG	1 cut(s)	1343
NdeII	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
NheI	GCTAGC	1 cut(s)	1178
NlaIII	CATG	2 cut(s)	1016, 2102
NlaIV	GGNNCC	8 cut(s)	13, 25, 57, 90, 681, 1161, 2117, 2382
NmeAIII	GCCGAG	1 cut(s)	1779
NmuCI	GTSAC	4 cut(s)	449, 607, 1383, 2014
NotI	GCGGCCGC	1 cut(s)	596
NspV	TTCGAA	3 cut(s)	404, 485, 2316
PaeR7I	CTCGAG	1 cut(s)	2440
PagI	TCATGA	1 cut(s)	1012
PcsI	WCGNNNNNNNCGW	2 cut(s)	233, 401
PctI	GAATGC	1 cut(s)	900
PdmI	GAANNNNTTC	2 cut(s)	1766, 2412
PfeI	GAWTC	8 cut(s)	293, 482, 487, 1489, 1546, 1871, 2066, 2338
PflFI	GACNNNGTC	2 cut(s)	229, 512
Ple19I	CGATCG	1 cut(s)	2227
PleI	GAGTC	5 cut(s)	532, 634, 868, 2096, 2436
PluTI	GGCGCC	2 cut(s)	15, 27
PpsI	GAGTC	5 cut(s)	532, 634, 868, 2096, 2436
PpuMI	RGGWCCY	2 cut(s)	56, 1160
Psp124BI	GAGCTC	2 cut(s)	210, 2503
Psp1406I	AACGTT	1 cut(s)	194
Psp5II	RGGWCCY	2 cut(s)	56, 1160
Psp6I	CCWGG	2 cut(s)	1066, 2276
PspEI	GGTNACC	1 cut(s)	1271
PspFI	CCCAGC	2 cut(s)	353, 1253
PspGI	CCWGG	2 cut(s)	1066, 2276
PspN4I	GGNNCC	8 cut(s)	13, 25, 57, 90, 681, 1161, 2117, 2382
PspPI	GGNCC	8 cut(s)	56, 169, 389, 1160, 1318, 1645, 1751, 2649
PspPPI	RGGWCCY	2 cut(s)	56, 1160
PspXI	VCTCGAGB	1 cut(s)	2440
PstNI	CAGNNNCTG	2 cut(s)	500, 2069
PsuI	RGATCY	1 cut(s)	1987
PsyI	GACNNNGTC	2 cut(s)	229, 512
PvuI	CGATCG	1 cut(s)	2227
PvuII	CAGCTG	2 cut(s)	970, 1826
RsaI	GTAC	6 cut(s)	32, 50, 1525, 2117, 2382, 2561
RsaNI	GTAC	6 cut(s)	31, 49, 1524, 2116, 2381, 2560
RseI	CAYNNNNRTG	1 cut(s)	1149
SacI	GAGCTC	2 cut(s)	210, 2503
SalI	GTCGAC	1 cut(s)	101
SaqAI	TTAA	8 cut(s)	993, 1061, 1533, 1964, 2387, 2469, 2610, 2630
Sau3AI	GATC	7 cut(s)	73, 250, 806, 1131, 1987, 2092, 2224
Sau96I	GGNCC	8 cut(s)	56, 169, 389, 1160, 1318, 1645, 1751, 2649
ScaI	AGTACT	2 cut(s)	32, 1525
SchI	GAGTC	5 cut(s)	533, 635, 869, 2096, 2437
ScrFI	CCNGG	4 cut(s)	1068, 1214, 2278, 2548
SduI	GDGCHC	4 cut(s)	210, 890, 1983, 2503
SfaNI	GCATC	4 cut(s)	577, 757, 1702, 2590
SfcI	CTRYAG	1 cut(s)	2677
SfoI	GGCGCC	2 cut(s)	13, 25
Sfr274I	CTCGAG	1 cut(s)	2440
SfuI	TTCGAA	3 cut(s)	404, 485, 2316
SgrAI	CRCCGGYG	1 cut(s)	601
SinI	GGWCC	3 cut(s)	56, 169, 1160
SlaI	CTCGAG	1 cut(s)	2440
SmiMI	CAYNNNNRTG	1 cut(s)	1149
SmlI	CTYRAG	6 cut(s)	209, 899, 1640, 1784, 2212, 2440
SmoI	CTYRAG	6 cut(s)	209, 899, 1640, 1784, 2212, 2440
SpeI	ACTAGT	1 cut(s)	2087
SspDI	GGCGCC	2 cut(s)	11, 23
SspI	AATATT	1 cut(s)	2615
SspMI	CTAG	6 cut(s)	891, 1179, 1923, 1943, 1991, 2088
SstI	GAGCTC	2 cut(s)	210, 2503
StyD4I	CCNGG	4 cut(s)	1066, 1212, 2276, 2546
TaaI	ACNGT	5 cut(s)	229, 301, 479, 2159, 2385
TaiI	ACGT	4 cut(s)	102, 197, 314, 2565
TaqII	GACCGA	1 cut(s)	2662
TatI	WGTACW	3 cut(s)	30, 48, 1523
TauI	GCSGC	4 cut(s)	122, 598, 601, 907
TfiI	GAWTC	8 cut(s)	293, 482, 487, 1489, 1546, 1871, 2066, 2338
Tru1I	TTAA	8 cut(s)	993, 1061, 1533, 1964, 2387, 2469, 2610, 2630
Tru9I	TTAA	8 cut(s)	993, 1061, 1533, 1964, 2387, 2469, 2610, 2630
TscAI	CASTG	5 cut(s)	120, 484, 1156, 2399, 2427
TseFI	GTSAC	4 cut(s)	449, 607, 1383, 2014
Tsp45I	GTSAC	4 cut(s)	449, 607, 1383, 2014
TspDTI	ATGAA	5 cut(s)	552, 660, 953, 2010, 2655
TspGWI	ACGGA	6 cut(s)	232, 322, 536, 537, 1735, 2557
TspRI	CASTG	5 cut(s)	120, 484, 1156, 2399, 2427
Tth111I	GACNNNGTC	2 cut(s)	229, 512
VpaK11BI	GGWCC	3 cut(s)	56, 169, 1160
XapI	RAATTY	3 cut(s)	108, 950, 1476
XbaI	TCTAGA	2 cut(s)	890, 1990
XcmI	CCANNNNNNNNNTGG	3 cut(s)	574, 1074, 1151
XhoI	CTCGAG	1 cut(s)	2440
XmiI	GTMKAC	2 cut(s)	102, 1633
XmnI	GAANNNNTTC	2 cut(s)	1766, 2412
XspI	CTAG	6 cut(s)	891, 1179, 1923, 1943, 1991, 2088
ZraI	GACGTC	1 cut(s)	312
ZrmI	AGTACT	2 cut(s)	32, 1525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.