Rh1DG336100

guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002708
Physical Location & Seq
Forward (+)
56171134 .. 56174082
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG336100.1

Sequence Viewer

Length: 1299 bp
ATGAATGGCATTAAAGGATCCATATGGGGCATGGCTGGAACAAAGCTTGTCTGTGCTGTCTTGTCACTGCCAGTCCCTTCTAGATCTTCAAATCCTATCATTCCCGAAATCATTGAGCAGAGAACACTTCAGAAGATACTTTTAGTTGGATGTAACGGATCTGGAGCAAGTACCATATTTAAACAGGCCAAGATTCTATATGAAGCCCCTTTTTCGGTGGATGAACGCGAAAGTATTAAGTCTACAATACAAACAAACGTGTATAAGTATGTTGGTATACTTCTTGAGGTTCGGGAACGTTTTGAAGAGGAATGTTTGGCTGAGATGAGGAGGCAATGCTCTAGTCAACCAGTTGCCAGTGGAGACAGCAACAAAACGCCCTACTCCATTGGCCCGAAGCTGAAAGCATTCTCTGATTGGCTTCTTAAGACTGTGGTGTCAGGCTATGTAGAGGCCATCTTCCAAGCTGCTACGCGTGAATTTGCACCATTTGTGGAGGAATTGTGGAATGATTCAGCCATTCAGGCCACATACAAGCGGTCAACTGAACTGGGAATGCTACCTAGTGTTGCTACTTATTTCATAAAGAGGGCTGTTCACATATTAAGGGTAAACTATGAGCCTACGGATGGAGACATCCTATATGCCGAAGATGATACTTCATCTACTGGGTTGGCTTGTGTGGACTTCTCATTGCCCCAGCCAGTATTGGGAAATGACTTCTATGGTCGTGATACTACTGATCAACATGACTCTTTGCTTAGGTACCAACTTGTTAGAGTAAATGCAGGACTTGGAGAAAGCTGCGAACAGTTGGACATGTTTGGAGATGTTGGAATAGTCATCTTCTGTGTGTCCTTGAGTGACTATGATCAATTTTCTGCTGACAGTAATGGGACTTTCACAAACAAGATGCTGCAGAGCAGATCTGTTTTTGAGAGAATCGTTACTCATCCCACCTTTGAGCAGATGAACTGCCTTTTGCTTCTCAACGAGTTTGATTTGTTTGTGAAAAAGATGGCACGGGTCCCTTTAACTCGATGCGAATGGTTTGATGATTTTAATCCAGTGATTAACAATGACCTGGGTCATCGAGCATATGAGTATATAGCAGTGAAGTTCAAGAGGCTCTATTCTTCTCTAACTGACAAGAGATTGTATGTTTCGCTGGTGGAGGACTTTGAACCTGGTGTACCTGAAGCTTTAAATTATTCAACGTGTTGTAAAACGAACATAAAAACTGTTTCAGAAAATGGAGAGAGCTTTGCTTTCAAGAACTTGAGAGAGAGAGAGAGTTAG

Protein Analysis

432

Amino Acids

48.67

Weight (kDa)

5.12

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 42 - 391 5.5e-61 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 436
Acc65I GGTACC 1 cut(s) 765
AccB1I GGYRCC 1 cut(s) 765
AccI GTMKAC 2 cut(s) 242, 277
AccII CGCG 2 cut(s) 228, 475
AciI CCGC 1 cut(s) 538
AclI AACGTT 1 cut(s) 298
AclWI GGATC 3 cut(s) 12, 25, 166
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 2 cut(s) 113, 1218
AfaI GTAC 3 cut(s) 172, 767, 1194
AfiI CCNNNNNNNGG 3 cut(s) 214, 629, 710
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 4 cut(s) 258, 473, 819, 1217
AgsI TTSAA 6 cut(s) 90, 305, 1123, 1184, 1215, 1273
AjnI CCWGG 2 cut(s) 1083, 1186
AluBI AGCT 6 cut(s) 46, 400, 467, 804, 1202, 1263
AluI AGCT 6 cut(s) 46, 400, 467, 804, 1202, 1263
Alw26I GTCTC 2 cut(s) 357, 627
AlwI GGATC 3 cut(s) 12, 25, 166
AoxI GGCC 4 cut(s) 186, 391, 453, 525
ApeKI GCWGC 3 cut(s) 467, 804, 916
ApoI RAATTY 1 cut(s) 479
Asp700I GAANNNNTTC 1 cut(s) 407
Asp718I GGTACC 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 392, 1027
AvaII GGWCC 1 cut(s) 1027
BamHI GGATCC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 765
BbvI GCAGC 3 cut(s) 454, 791, 903
BccI CCATC 3 cut(s) 464, 623, 1012
BciT130I CCWGG 2 cut(s) 1085, 1188
BclI TGATCA 2 cut(s) 742, 871
BcoDI GTCTC 2 cut(s) 357, 627
BfaI CTAG 3 cut(s) 81, 342, 564
BfmI CTRYAG 1 cut(s) 917
BfrI CTTAAG 1 cut(s) 425
BglI GCCNNNNNGGC 1 cut(s) 524
BglII AGATCT 2 cut(s) 83, 926
BisI GCNGC 3 cut(s) 468, 805, 917
BlsI GCNGC 3 cut(s) 469, 806, 918
Bme1390I CCNGG 2 cut(s) 1085, 1188
Bme18I GGWCC 1 cut(s) 1027
BmgT120I GGNCC 2 cut(s) 392, 1027
BmiI GGNNCC 4 cut(s) 19, 767, 1028, 1029
BmrFI CCNGG 2 cut(s) 1085, 1188
BmrI ACTGGG 2 cut(s) 560, 678
BmsI GCATC 2 cut(s) 903, 1031
BmuI ACTGGG 2 cut(s) 560, 678
BoxI GACNNNNGTC 1 cut(s) 1086
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 761
BpuEI CTTGAG 2 cut(s) 305, 880
BsaBI GATNNNNATC 1 cut(s) 1062
BsaJI CCNNGG 1 cut(s) 1084
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 629, 710
Bse1I ACTGG 7 cut(s) 71, 350, 357, 555, 673, 704, 1067
Bse3DI GCAATG 2 cut(s) 341, 692
Bse8I GATNNNNATC 1 cut(s) 1062
BseBI CCWGG 2 cut(s) 1085, 1188
BseDI CCNNGG 1 cut(s) 1084
BseGI GGATG 5 cut(s) 155, 226, 634, 636, 952
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 3 cut(s) 214, 629, 710
BseMI GCAATG 2 cut(s) 341, 692
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 7 cut(s) 71, 350, 357, 555, 673, 704, 1067
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 3 cut(s) 454, 791, 903
BseYI CCCAGC 1 cut(s) 699
Bsh1236I CGCG 2 cut(s) 228, 475
BshFI GGCC 4 cut(s) 188, 393, 455, 527
BshNI GGYRCC 1 cut(s) 765
BslFI GGGAC 3 cut(s) 59, 910, 1013
BslI CCNNNNNNNGG 3 cut(s) 214, 629, 710
BsmAI GTCTC 2 cut(s) 357, 627
BsmFI GGGAC 3 cut(s) 59, 910, 1013
BsmI GAATGC 2 cut(s) 407, 561
BsnI GGCC 4 cut(s) 188, 393, 455, 527
Bsp143I GATC 6 cut(s) 17, 83, 158, 742, 871, 926
BspACI CCGC 1 cut(s) 538
BspANI GGCC 4 cut(s) 188, 393, 455, 527
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 2 cut(s) 228, 475
BspLI GGNNCC 4 cut(s) 19, 767, 1028, 1029
BspMAI CTGCAG 1 cut(s) 921
BspPI GGATC 3 cut(s) 12, 25, 166
BspT107I GGYRCC 1 cut(s) 765
BspTI CTTAAG 1 cut(s) 425
BsrDI GCAATG 2 cut(s) 341, 692
BsrI ACTGG 7 cut(s) 71, 350, 357, 555, 673, 704, 1067
BssECI CCNNGG 1 cut(s) 1084
BssMI GATC 6 cut(s) 17, 83, 158, 742, 871, 926
BssNAI GTATAC 1 cut(s) 278
Bst1107I GTATAC 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 1085, 1188
Bst4CI ACNGT 4 cut(s) 433, 813, 890, 1243
Bst6I CTCTTC 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 425
BstDEI CTNAG 2 cut(s) 321, 761
BstF5I GGATG 5 cut(s) 155, 226, 634, 636, 952
BstFNI CGCG 2 cut(s) 228, 475
BstKTI GATC 6 cut(s) 20, 86, 161, 745, 874, 929
BstMAI GTCTC 2 cut(s) 357, 627
BstMBI GATC 6 cut(s) 17, 83, 158, 742, 871, 926
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstNI CCWGG 2 cut(s) 1085, 1188
BstNSI RCATGY 1 cut(s) 823
BstPAI GACNNNNGTC 1 cut(s) 1086
BstSCI CCNGG 2 cut(s) 1083, 1186
BstSFI CTRYAG 1 cut(s) 917
BstUI CGCG 2 cut(s) 228, 475
BstV1I GCAGC 3 cut(s) 454, 791, 903
BstX2I RGATCY 4 cut(s) 17, 83, 158, 926
BstYI RGATCY 4 cut(s) 17, 83, 158, 926
BstZ17I GTATAC 1 cut(s) 278
BsuRI GGCC 4 cut(s) 188, 393, 455, 527
BtsCI GGATG 5 cut(s) 155, 226, 634, 636, 952
BtsI GCAGTG 2 cut(s) 65, 1119
BtsIMutI CAGTG 4 cut(s) 65, 364, 1074, 1119
Cfr13I GGNCC 2 cut(s) 392, 1027
CsiI ACCWGGT 1 cut(s) 1186
Csp6I GTAC 3 cut(s) 171, 766, 1193
CviAII CATG 3 cut(s) 31, 749, 820
CviQI GTAC 3 cut(s) 171, 766, 1193
DdeI CTNAG 2 cut(s) 321, 761
DpnI GATC 6 cut(s) 19, 85, 160, 744, 873, 928
DpnII GATC 6 cut(s) 17, 83, 158, 742, 871, 926
DraI TTTAAA 2 cut(s) 181, 1206
DrdI GACNNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 1027
Eco57I CTGAAG 2 cut(s) 113, 1218
EcoO109I RGGNCCY 1 cut(s) 1027
EcoRII CCWGG 2 cut(s) 1083, 1186
FaeI CATG 3 cut(s) 34, 752, 823
FaqI GGGAC 3 cut(s) 59, 910, 1013
FatI CATG 3 cut(s) 30, 748, 819
FauNDI CATATG 2 cut(s) 23, 1099
FbaI TGATCA 2 cut(s) 742, 871
FblI GTMKAC 2 cut(s) 242, 277
Fnu4HI GCNGC 3 cut(s) 468, 805, 917
FokI GGATG 5 cut(s) 162, 233, 623, 641, 939
Fsp4HI GCNGC 3 cut(s) 468, 805, 917
FspBI CTAG 3 cut(s) 81, 342, 564
GluI GCNGC 3 cut(s) 468, 805, 917
GsaI CCCAGC 1 cut(s) 703
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 4 cut(s) 188, 393, 455, 527
Hin1II CATG 3 cut(s) 34, 752, 823
HincII GTYRAC 2 cut(s) 347, 543
HindII GTYRAC 2 cut(s) 347, 543
HindIII AAGCTT 2 cut(s) 44, 1200
HinfI GANTC 4 cut(s) 193, 512, 752, 942
Hpy166II GTNNAC 8 cut(s) 243, 278, 347, 543, 598, 613, 685, 1193
Hpy188I TCNGA 3 cut(s) 132, 415, 1249
Hpy188III TCNNGA 8 cut(s) 81, 104, 162, 284, 293, 731, 1123, 1273
Hpy8I GTNNAC 8 cut(s) 243, 278, 347, 543, 598, 613, 685, 1193
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 4 cut(s) 433, 813, 890, 1243
HpyCH4IV ACGT 3 cut(s) 258, 298, 1217
HpyCH4V TGCA 3 cut(s) 485, 788, 919
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpyF3I CTNAG 2 cut(s) 321, 761
HpySE526I ACGT 3 cut(s) 258, 298, 1217
Hsp92II CATG 3 cut(s) 34, 752, 823
KflI GGGWCCC 1 cut(s) 1027
KpnI GGTACC 1 cut(s) 769
Ksp22I TGATCA 2 cut(s) 742, 871
Kzo9I GATC 6 cut(s) 17, 83, 158, 742, 871, 926
LmnI GCTCC 1 cut(s) 164
Lsp1109I GCAGC 3 cut(s) 454, 791, 903
LweI GCATC 2 cut(s) 903, 1031
MabI ACCWGGT 1 cut(s) 1186
MaeI CTAG 3 cut(s) 81, 342, 564
MaeII ACGT 3 cut(s) 258, 298, 1217
MaeIII GTNAC 4 cut(s) 63, 152, 863, 946
MalI GATC 6 cut(s) 19, 85, 160, 744, 873, 928
MboI GATC 6 cut(s) 17, 83, 158, 742, 871, 926
MboII GAAGA 7 cut(s) 78, 145, 317, 451, 662, 838, 1128
MflI RGATCY 4 cut(s) 17, 83, 158, 926
MluCI AATT 4 cut(s) 479, 500, 875, 1207
MluI ACGCGT 1 cut(s) 473
MlyI GAGTC 1 cut(s) 746
MmeI TCCRAC 3 cut(s) 127, 795, 814
MnlI CCTC 9 cut(s) 280, 301, 321, 324, 445, 490, 582, 1119, 1168
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 9 cut(s) 12, 180, 237, 426, 605, 1034, 1062, 1074, 1205
MspCI CTTAAG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 1085, 1188
Mva1269I GAATGC 2 cut(s) 407, 561
MvaI CCWGG 2 cut(s) 1085, 1188
MvnI CGCG 2 cut(s) 228, 475
MwoI GCNNNNNNNGC 1 cut(s) 524
NdeI CATATG 2 cut(s) 23, 1099
NdeII GATC 6 cut(s) 17, 83, 158, 742, 871, 926
NlaIII CATG 3 cut(s) 34, 752, 823
NlaIV GGNNCC 4 cut(s) 19, 767, 1028, 1029
NmuCI GTSAC 2 cut(s) 63, 863
NspI RCATGY 1 cut(s) 823
PciI ACATGT 1 cut(s) 819
PctI GAATGC 2 cut(s) 407, 561
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 3 cut(s) 193, 512, 942
PkrI GCNGC 3 cut(s) 469, 806, 918
PleI GAGTC 1 cut(s) 746
PpsI GAGTC 1 cut(s) 746
PpuMI RGGWCCY 1 cut(s) 1027
PscI ACATGT 1 cut(s) 819
PshAI GACNNNNGTC 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 1027
Psp6I CCWGG 2 cut(s) 1083, 1186
PspFI CCCAGC 1 cut(s) 699
PspGI CCWGG 2 cut(s) 1083, 1186
PspN4I GGNNCC 4 cut(s) 19, 767, 1028, 1029
PspPI GGNCC 2 cut(s) 392, 1027
PspPPI RGGWCCY 1 cut(s) 1027
PsrI GAACNNNNNNTAC 2 cut(s) 1176, 1208
PstI CTGCAG 1 cut(s) 921
PsuI RGATCY 4 cut(s) 17, 83, 158, 926
RsaI GTAC 3 cut(s) 172, 767, 1194
RsaNI GTAC 3 cut(s) 171, 766, 1193
SaqAI TTAA 9 cut(s) 12, 180, 237, 426, 605, 1034, 1062, 1074, 1205
SatI GCNGC 3 cut(s) 468, 805, 917
Sau3AI GATC 6 cut(s) 17, 83, 158, 742, 871, 926
Sau96I GGNCC 2 cut(s) 392, 1027
SchI GAGTC 1 cut(s) 746
ScrFI CCNGG 2 cut(s) 1085, 1188
SexAI ACCWGGT 1 cut(s) 1186
SfaNI GCATC 2 cut(s) 903, 1031
SfcI CTRYAG 1 cut(s) 917
SinI GGWCC 1 cut(s) 1027
SmlI CTYRAG 4 cut(s) 284, 425, 859, 1279
SmoI CTYRAG 4 cut(s) 284, 425, 859, 1279
Sse9I AATT 4 cut(s) 479, 500, 875, 1207
SsiI CCGC 1 cut(s) 538
SspMI CTAG 3 cut(s) 81, 342, 564
StyD4I CCNGG 2 cut(s) 1083, 1186
TaaI ACNGT 4 cut(s) 433, 813, 890, 1243
TaiI ACGT 3 cut(s) 261, 301, 1220
TaqI TCGA 2 cut(s) 1039, 1093
TasI AATT 4 cut(s) 479, 500, 875, 1207
TfiI GAWTC 3 cut(s) 193, 512, 942
Tru1I TTAA 9 cut(s) 12, 180, 237, 426, 605, 1034, 1062, 1074, 1205
Tru9I TTAA 9 cut(s) 12, 180, 237, 426, 605, 1034, 1062, 1074, 1205
TscAI CASTG 4 cut(s) 72, 364, 1074, 1119
TseFI GTSAC 2 cut(s) 63, 863
TseI GCWGC 3 cut(s) 467, 804, 916
Tsp45I GTSAC 2 cut(s) 63, 863
TspDTI ATGAA 6 cut(s) 17, 216, 237, 571, 651, 986
TspGWI ACGGA 2 cut(s) 171, 641
TspRI CASTG 4 cut(s) 72, 364, 1074, 1119
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 1027
XapI RAATTY 1 cut(s) 479
XbaI TCTAGA 1 cut(s) 80
XceI RCATGY 1 cut(s) 823
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 2 cut(s) 242, 277
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 3 cut(s) 81, 342, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.