Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002708

Physical Location & Seq

Genomic Coordinates Forward (+)

13398447 .. 13402523

Gene Structure 4077 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1159500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGCCTTCCGACGCCGACAGTGGGGTCCAGTACTCGTTCGCGATAGAGTACAACGGTCCTCCCATCAGTTACGATCTCCCGCGGGCGGTTCCGATTAACATCGATAGGATTCCGGTGGCGGCTGTGGTCGGAGGGGTTGCGCTGCCCGAGAAGCTGACCCTGCCGGTGGTCCAGCCGGTGATGGCGCCCGCAACTTTCTCGAAAGAGCTCAAGGCGTCTAAATCGACGGTCTCTCCGACTTCCGTTTTCGATCGGGGCAGCGAGGATTACACGAAGGAGTTGGAAGGTTCTGAATCGACGGTTTCGCCGACGTCGGTGATTGGATTTGAGGAGAGGGGTGAGGAGAGTGTGGTTGGGTGTGCGTTGTCCGGTGAATTGAGTAGCTCCGGCGCATCGGAGTTTTCGAACGGGTTGAATTACAGGTCGGGTGAGTTTTCCGATGTGATTGGTGGCGGGAAGGAGAGTGTGGACTTCAACAGCGATTCGAACCGGCCCGAACCGGAACAGGATTGGGCGTTCACGGAGTCCGTTTTGAGCTTGGACTATCCATCGTCTCGGGTTTCATCCAGTAAGGCTTTGGATTATGACGCACTGCGGCCGCCGGTGGTGACTTTTCGGGACATTGAATCGGACGATGGAGCTGATGAAGACGAGGCTGAAGTTGTGCAGGCAAAGCGGGAGCCTCAGAGCAAAGGGAAGAAGAAGGCGTGCTATAGATGTTTGAAGGGGACGAGGTTTACTGAGAAGGAGGTTTGCATTGTCTGTGATGCTAAGTATTGTAGTAGTTGTGTGCTTAGAGCAATGGGGTCGATGCCCGAAGGTCGAAAATGTGTGAGTTGCATTGGGTTTCCTATTGACGAGTCGAAAAGAGGGAGCTTGGGGAAGTGCTCTAGAATGCTCAAGCGGCTGTTGAATGATTTGGAGGTTAGACAGGTGATGAAAACCGAAAAGTTTTGTGAGGCGAATCAGCTGCCTTCGGACTATATTTGTGTGAATGGACAGCCTCTTTGTCCGGAGGAGCTGGTTCTATTACAAACCTGCCCAAATCCGCCGAAGAAATTAAAACCAGGGAACTATTGGTATGATAAAGTTTCTGGTCTTTGGGGAAAGGAAGGACAGAAGCCTTCCCAGATAATTAGTCCCCATCTTAGTGTTGGGGGTCCCATCGAAGCAAATGCTAGCAATGGAAACACACAGGTTTATATAAATGGTCGGGAAATTACCAAAGTCGAGCTTCGGATGTTGCAGTTGGCTGGGGTTCAATGTGCTGGTAAACCACACTTTTGGGTAAACGAGGACGGTTCATACCAAGAGGAGGGCCAGAAAAATACTAAAGGATACATTTGGGGCAAGGCTGGAACAAAGCTTGTTTGTGCCGCCTTGTCATTACCAGTCCCTTCTAAATCTTCAACTCCTTGTGGAGAATCCCTGAACTATGTGGGTAGTAGAGTCGTCCCTGATTACATTGAGCAGAGAATACTTCAGAAGATTCTTTTAGTTGGATATAATGGTTCTGGAACAAGTACTATATTTAAACAGGCAAAGATTCTATATAAAGCTGTCCCTTTCTTGGAGGATGAGCGTGAAAATATCAAATGTACAATACAGAGTAACGTGTATGGTTATCTTGGTATGCTTCTTGAGGGCCGTGAGCGTTTTGAAGAGGAATGTTTGGATGGGATGAGGAAGCAATGTTCGTCCAGTCAAACAGATGCCAACGGAAACAATGAGAAAACTCTTTACTCCATTGGCCCAAGGCTGAAAGCGTTCTCTGATTGGCTTCTCAAGACTATGGTGTCAGGCAATCTGGAAGCCATTTTTCCAGCGGCTACTCGTGAATATGCACCATTAGTTGAGGAATTGTGGAATGATTCAGCCATTCAGGTCACATACAAGCGGAGAAATGAATTGGAAATGCTACCTAGTGTTGCTACTTATTTCTTAGAGAGGGCTGTTGACATATTAAGAATAGGCTACGAGCCCACAGATCTAGATATATTATATGCTGAAGGTGTCACTTCATCTAATGGGCTTGCTTGTGTGGACTTCTCATTTCCCAACGCAGAATCTGAAGACACCATCAATACTACTGACCAGCATGACTCTTTGCTTAGGTATGTACCAACTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009898 GO:0009987 GO:0016020 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0030234 GO:0030695 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0034260 GO:0035639 GO:0036094 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0048519 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051346 GO:0051716 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

711

Amino Acids

78.07

Weight (kDa)

4.97

Isoelectric Point (pI)

48.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	495 - 687	2.5e-29	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23460
fragaria_vesca	FvH4_1g14590 FvH4_1g14590
malus_domestica	MD00G1067300.v1.1 MD02G1159500.v1.1
prunus_persica	Prupe.7G144400_v2.0.a1
pyrus_communis	pycom02g12580 pycom15g23720
rosa_chinensis	RchiOBHm_Chr1g0367271 RchiOBHm_Chr1g0367281 RchiOBHm_Chr1g0367331 RchiOBHm_Chr2g0103371
rosa_laevigata	RLG00000017231 RLG00000027279 RLG00000027284
rosa_multiflora	Rmu_sc0001555.1_g000003 Rmu_sc0002434.1_g000010 Rmu_sc0011000.1_g000013
rosa_roxburghii	Rroxscaffold_2G00139680 Rroxscaffold_4G00289390 Rroxscaffold_4G00289470 Rroxscaffold_4G00289480
rosa_rugosa	Rorug02G0114700 Rorug06G0002300
rosa_samantha	Rh1AG342600 Rh1BG303200 Rh1BG303600 Rh1BG303700 Rh1CG319500 Rh1CG319700 Rh1DG335600 Rh1DG336000 Rh1DG336100 Rh2AG162700 Rh2BG169800 Rh2CG169900 Rh2DG168800
rosa_wichuraiana	Rw1G030440 Rw2G012780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	23, 1795
AatII	GACGTC	1 cut(s)	314
Acc36I	ACCTGC	1 cut(s)	1046
AccB1I	GGYRCC	1 cut(s)	184
AccII	CGCG	2 cut(s)	41, 82
AccIII	TCCGGA	1 cut(s)	1012
AcoI	YGGCCR	1 cut(s)	596
AcuI	CTGAAG	4 cut(s)	678, 1466, 2028, 2091
AcyI	GRCGYC	4 cut(s)	12, 185, 215, 311
AfaI	GTAC	5 cut(s)	32, 50, 1525, 1600, 2121
AfiI	CCNNNNNNNGG	5 cut(s)	21, 85, 166, 1315, 1570
AflIII	ACRYGT	1 cut(s)	1614
AgsI	TTSAA	8 cut(s)	415, 475, 626, 724, 913, 1262, 1410, 1661
AjnI	CCWGG	1 cut(s)	1066
AloI	GAACNNNNNNTCC	4 cut(s)	500, 532, 1678, 1710
Alw21I	GWGCWC	2 cut(s)	210, 890
Alw26I	GTCTC	2 cut(s)	235, 558
AlwNI	CAGNNNCTG	1 cut(s)	2069
Ama87I	CYCGRG	2 cut(s)	146, 555
Aor13HI	TCCGGA	1 cut(s)	1012
AoxI	GGCC	5 cut(s)	491, 596, 1318, 1645, 1750
ApeKI	GCWGC	3 cut(s)	142, 258, 970
ArsI	GACNNNNNNTTYG	2 cut(s)	990, 1022
Asp700I	GAANNNNTTC	1 cut(s)	1766
AspLEI	GCGC	3 cut(s)	142, 187, 392
AspS9I	GGNCC	8 cut(s)	25, 56, 169, 492, 1160, 1318, 1645, 1751
AsuHPI	GGTGA	7 cut(s)	190, 328, 350, 383, 440, 619, 946
AsuII	TTCGAA	2 cut(s)	404, 485
AsuNHI	GCTAGC	1 cut(s)	1178
AvaI	CYCGRG	2 cut(s)	146, 555
AvaII	GGWCC	4 cut(s)	25, 56, 169, 1160
BanI	GGYRCC	1 cut(s)	184
BanII	GRGCYC	2 cut(s)	210, 1983
BauI	CACGAG	1 cut(s)	1833
BbsI	GAAGAC	2 cut(s)	654, 2079
Bbv12I	GWGCWC	2 cut(s)	210, 890
BbvI	GCAGC	3 cut(s)	129, 270, 957
BccI	CCATC	8 cut(s)	71, 175, 556, 629, 1152, 1172, 1670, 2087
BceAI	ACGGC	1 cut(s)	1632
BcgI	CGANNNNNNTGC	8 cut(s)	180, 214, 789, 823, 952, 986, 1157, 1191
BciT130I	CCWGG	1 cut(s)	1068
BciVI	GTATCC	1 cut(s)	1331
BcoDI	GTCTC	2 cut(s)	235, 558
BfaI	CTAG	4 cut(s)	891, 1179, 1923, 1991
BfmI	CTRYAG	1 cut(s)	712
BfoI	RGCGCY	1 cut(s)	188
BfuAI	ACCTGC	1 cut(s)	1046
BfuI	GTATCC	1 cut(s)	1331
BglII	AGATCT	1 cut(s)	1987
BisI	GCNGC	9 cut(s)	120, 143, 259, 596, 599, 905, 971, 1377, 1827
BlsI	GCNGC	9 cut(s)	121, 144, 260, 597, 600, 906, 972, 1378, 1828
BmcAI	AGTACT	2 cut(s)	32, 1525
Bme1390I	CCNGG	1 cut(s)	1068
Bme18I	GGWCC	4 cut(s)	25, 56, 169, 1160
BmeT110I	CYCGRG	2 cut(s)	146, 555
BmgT120I	GGNCC	8 cut(s)	25, 56, 169, 492, 1160, 1318, 1645, 1751
BmiI	GGNNCC	6 cut(s)	26, 90, 186, 681, 1161, 1162
BmrFI	CCNGG	1 cut(s)	1068
BmsI	GCATC	4 cut(s)	401, 757, 801, 1702
BmtI	GCTAGC	1 cut(s)	1182
BpiI	GAAGAC	2 cut(s)	654, 2079
Bpu10I	CCTNAGC	1 cut(s)	2111
Bpu14I	TTCGAA	2 cut(s)	404, 485
BpuEI	CTTGAG	4 cut(s)	194, 884, 1661, 1769
Bsa29I	ATCGAT	1 cut(s)	102
BsaBI	GATNNNNATC	1 cut(s)	98
BsaHI	GRCGYC	4 cut(s)	12, 185, 215, 311
BsaI	GGTCTC	1 cut(s)	235
BsaJI	CCNNGG	3 cut(s)	80, 1067, 1754
BsaWI	WCCGGW	4 cut(s)	112, 368, 499, 1012
BsaXI	ACNNNNNCTCC	2 cut(s)	217, 247
Bsc4I	CCNNNNNNNGG	5 cut(s)	21, 85, 166, 1315, 1570
Bse118I	RCCGGY	4 cut(s)	163, 175, 489, 601
Bse1I	ACTGG	4 cut(s)	28, 567, 1391, 1701
Bse3DI	GCAATG	3 cut(s)	807, 1189, 1697
Bse8I	GATNNNNATC	1 cut(s)	98
BseAI	TCCGGA	1 cut(s)	1012
BseBI	CCWGG	1 cut(s)	1068
BseCI	ATCGAT	1 cut(s)	102
BseDI	CCNNGG	3 cut(s)	80, 1067, 1754
BseGI	GGATG	5 cut(s)	563, 1245, 1582, 1681, 1686
BseJI	GATNNNNATC	1 cut(s)	98
BseLI	CCNNNNNNNGG	5 cut(s)	21, 85, 166, 1315, 1570
BseMI	GCAATG	3 cut(s)	807, 1189, 1697
BseMII	CTCAG	2 cut(s)	698, 732
BseNI	ACTGG	4 cut(s)	28, 567, 1391, 1701
BseRI	GAGGAG	4 cut(s)	344, 356, 1031, 1328
BseX3I	CGGCCG	1 cut(s)	596
BseXI	GCAGC	3 cut(s)	129, 270, 957
BseYI	CCCAGC	1 cut(s)	1253
BsgI	GTGCAG	1 cut(s)	686
Bsh1236I	CGCG	2 cut(s)	41, 82
Bsh1285I	CGRYCG	2 cut(s)	253, 599
BshFI	GGCC	5 cut(s)	493, 598, 1320, 1647, 1752
BshNI	GGYRCC	1 cut(s)	184
BshVI	ATCGAT	1 cut(s)	102
BsiEI	CGRYCG	2 cut(s)	253, 599
BsiHKAI	GWGCWC	2 cut(s)	210, 890
BsiHKCI	CYCGRG	2 cut(s)	146, 555
BsiSI	CCGG	9 cut(s)	113, 164, 176, 369, 387, 490, 500, 602, 1013
BslFI	GGGAC	7 cut(s)	632, 742, 1125, 1146, 1379, 1439, 1547
BslI	CCNNNNNNNGG	5 cut(s)	21, 85, 166, 1315, 1570
BsmAI	GTCTC	2 cut(s)	235, 558
BsmBI	CGTCTC	1 cut(s)	558
BsmFI	GGGAC	7 cut(s)	632, 742, 1125, 1146, 1379, 1439, 1547
BsmI	GAATGC	1 cut(s)	900
BsnI	GGCC	5 cut(s)	493, 598, 1320, 1647, 1752
Bso31I	GGTCTC	1 cut(s)	235
BsoBI	CYCGRG	2 cut(s)	146, 555
Bsp119I	TTCGAA	2 cut(s)	404, 485
Bsp1286I	GDGCHC	3 cut(s)	210, 890, 1983
Bsp13I	TCCGGA	1 cut(s)	1012
Bsp1407I	TGTACA	1 cut(s)	1598
Bsp143I	GATC	3 cut(s)	73, 250, 1987
Bsp68I	TCGCGA	1 cut(s)	41
BspANI	GGCC	5 cut(s)	493, 598, 1320, 1647, 1752
BspCNI	CTCAG	2 cut(s)	697, 733
BspDI	ATCGAT	1 cut(s)	102
BspEI	TCCGGA	1 cut(s)	1012
BspFNI	CGCG	2 cut(s)	41, 82
BspLI	GGNNCC	6 cut(s)	26, 90, 186, 681, 1161, 1162
BspMI	ACCTGC	1 cut(s)	1046
BspOI	GCTAGC	1 cut(s)	1182
BspT104I	TTCGAA	2 cut(s)	404, 485
BspT107I	GGYRCC	1 cut(s)	184
BspTNI	GGTCTC	1 cut(s)	235
BsrDI	GCAATG	3 cut(s)	807, 1189, 1697
BsrFI	RCCGGY	4 cut(s)	163, 175, 489, 601
BsrGI	TGTACA	1 cut(s)	1598
BsrI	ACTGG	4 cut(s)	28, 567, 1391, 1701
BssAI	RCCGGY	4 cut(s)	163, 175, 489, 601
BssECI	CCNNGG	3 cut(s)	80, 1067, 1754
BssMI	GATC	3 cut(s)	73, 250, 1987
BssNI	GRCGYC	4 cut(s)	12, 185, 215, 311
BssSI	CACGAG	1 cut(s)	1833
BssT1I	CCWWGG	1 cut(s)	1754
Bst2BI	CACGAG	1 cut(s)	1833
Bst2UI	CCWGG	1 cut(s)	1068
Bst4CI	ACNGT	5 cut(s)	20, 56, 229, 301, 1301
Bst6I	CTCTTC	1 cut(s)	1656
BstACI	GRCGYC	4 cut(s)	12, 185, 215, 311
BstAUI	TGTACA	1 cut(s)	1598
BstBI	TTCGAA	2 cut(s)	404, 485
BstC8I	GCNNGC	6 cut(s)	84, 189, 669, 709, 1180, 2034
BstDEI	CTNAG	7 cut(s)	684, 741, 771, 794, 1148, 1942, 2111
BstDSI	CCRYGG	1 cut(s)	80
BstF5I	GGATG	5 cut(s)	563, 1245, 1582, 1681, 1686
BstFNI	CGCG	2 cut(s)	41, 82
BstH2I	RGCGCY	1 cut(s)	188
BstHHI	GCGC	3 cut(s)	142, 187, 392
BstKTI	GATC	3 cut(s)	76, 253, 1990
BstMAI	GTCTC	2 cut(s)	235, 558
BstMBI	GATC	3 cut(s)	73, 250, 1987
BstMCI	CGRYCG	2 cut(s)	253, 599
BstMWI	GCNNNNNNNGC	4 cut(s)	151, 160, 673, 904
BstNI	CCWGG	1 cut(s)	1068
BstSCI	CCNGG	1 cut(s)	1066
BstSFI	CTRYAG	1 cut(s)	712
BstUI	CGCG	2 cut(s)	41, 82
BstV1I	GCAGC	3 cut(s)	129, 270, 957
BstV2I	GAAGAC	2 cut(s)	654, 2079
BstX2I	RGATCY	1 cut(s)	1987
BstXI	CCANNNNNNTGG	1 cut(s)	1284
BstYI	RGATCY	1 cut(s)	1987
BstZI	CGGCCG	1 cut(s)	596
Bsu15I	ATCGAT	1 cut(s)	102
BsuI	GTATCC	1 cut(s)	1331
BsuRI	GGCC	5 cut(s)	493, 598, 1320, 1647, 1752
BsuTUI	ATCGAT	1 cut(s)	102
BtgI	CCRYGG	1 cut(s)	80
BtsCI	GGATG	5 cut(s)	563, 1245, 1582, 1681, 1686
BtsI	GCAGTG	1 cut(s)	590
BtsIMutI	CAGTG	2 cut(s)	25, 590
BtuMI	TCGCGA	1 cut(s)	41
BveI	ACCTGC	1 cut(s)	1046
Cac8I	GCNNGC	6 cut(s)	84, 189, 669, 709, 1180, 2034
CaiI	CAGNNNCTG	1 cut(s)	2069
CciNI	GCGGCCGC	1 cut(s)	596
CfoI	GCGC	3 cut(s)	142, 187, 392
Cfr10I	RCCGGY	4 cut(s)	163, 175, 489, 601
Cfr13I	GGNCC	8 cut(s)	25, 56, 169, 492, 1160, 1318, 1645, 1751
Cfr42I	CCGCGG	1 cut(s)	83
ClaI	ATCGAT	1 cut(s)	102
CseI	GACGC	3 cut(s)	20, 204, 596
Csp6I	GTAC	5 cut(s)	31, 49, 1524, 1599, 2120
CviAII	CATG	1 cut(s)	2099
CviQI	GTAC	5 cut(s)	31, 49, 1524, 1599, 2120
DdeI	CTNAG	7 cut(s)	684, 741, 771, 794, 1148, 1942, 2111
DinI	GGCGCC	1 cut(s)	186
DpnI	GATC	3 cut(s)	75, 252, 1989
DpnII	GATC	3 cut(s)	73, 250, 1987
DraI	TTTAAA	1 cut(s)	1534
DrdI	GACNNNNNNGTC	2 cut(s)	23, 1795
DseDI	GACNNNNNNGTC	2 cut(s)	23, 1795
EaeI	YGGCCR	1 cut(s)	596
EagI	CGGCCG	1 cut(s)	596
Eam1104I	CTCTTC	1 cut(s)	1656
EarI	CTCTTC	1 cut(s)	1656
EciI	GGCGGA	1 cut(s)	1038
Ecl136II	GAGCTC	1 cut(s)	208
EclXI	CGGCCG	1 cut(s)	596
Eco130I	CCWWGG	1 cut(s)	1754
Eco24I	GRGCYC	2 cut(s)	210, 1983
Eco31I	GGTCTC	1 cut(s)	235
Eco47I	GGWCC	4 cut(s)	25, 56, 169, 1160
Eco52I	CGGCCG	1 cut(s)	596
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	4 cut(s)	678, 1466, 2028, 2091
Eco88I	CYCGRG	2 cut(s)	146, 555
EcoICRI	GAGCTC	1 cut(s)	208
EcoO109I	RGGNCCY	1 cut(s)	1160
EcoRII	CCWGG	1 cut(s)	1066
EcoT14I	CCWWGG	1 cut(s)	1754
EcoT38I	GRGCYC	2 cut(s)	210, 1983
EgeI	GGCGCC	1 cut(s)	186
EheI	GGCGCC	1 cut(s)	186
ErhI	CCWWGG	1 cut(s)	1754
Esp3I	CGTCTC	1 cut(s)	558
FaeI	CATG	1 cut(s)	2102
FalI	AAGNNNNNCTT	2 cut(s)	1218, 1250
FaqI	GGGAC	7 cut(s)	632, 742, 1125, 1146, 1379, 1439, 1547
FatI	CATG	1 cut(s)	2098
FauI	CCCGC	5 cut(s)	75, 87, 196, 446, 669
Fnu4HI	GCNGC	9 cut(s)	120, 143, 259, 596, 599, 905, 971, 1377, 1827
FokI	GGATG	5 cut(s)	550, 1252, 1589, 1688, 1693
FriOI	GRGCYC	2 cut(s)	210, 1983
Fsp4HI	GCNGC	9 cut(s)	120, 143, 259, 596, 599, 905, 971, 1377, 1827
FspBI	CTAG	4 cut(s)	891, 1179, 1923, 1991
GlaI	GCGC	3 cut(s)	141, 186, 391
GluI	GCNGC	9 cut(s)	120, 143, 259, 596, 599, 905, 971, 1377, 1827
GsaI	CCCAGC	1 cut(s)	1257
HaeII	RGCGCY	1 cut(s)	188
HaeIII	GGCC	5 cut(s)	493, 598, 1320, 1647, 1752
HapII	CCGG	9 cut(s)	113, 164, 176, 369, 387, 490, 500, 602, 1013
HgaI	GACGC	3 cut(s)	20, 204, 596
HhaI	GCGC	3 cut(s)	142, 187, 392
Hin1I	GRCGYC	4 cut(s)	12, 185, 215, 311
Hin1II	CATG	1 cut(s)	2102
Hin6I	GCGC	3 cut(s)	140, 185, 390
HinP1I	GCGC	3 cut(s)	140, 185, 390
HincII	GTYRAC	1 cut(s)	1957
HindII	GTYRAC	1 cut(s)	1957
HindIII	AAGCTT	1 cut(s)	1364
HpaII	CCGG	9 cut(s)	113, 164, 176, 369, 387, 490, 500, 602, 1013
HphI	GGTGA	7 cut(s)	190, 328, 350, 383, 440, 619, 946
Hpy166II	GTNNAC	7 cut(s)	469, 519, 738, 1274, 1291, 1957, 2044
Hpy8I	GTNNAC	7 cut(s)	469, 519, 738, 1274, 1291, 1957, 2044
Hpy99I	CGWCG	5 cut(s)	14, 229, 301, 313, 316
HpyCH4III	ACNGT	5 cut(s)	20, 56, 229, 301, 1301
HpyCH4IV	ACGT	2 cut(s)	311, 1614
HpyCH4V	TGCA	5 cut(s)	667, 756, 840, 1246, 1844
HpyF10VI	GCNNNNNNNGC	4 cut(s)	151, 160, 673, 904
HpyF3I	CTNAG	7 cut(s)	684, 741, 771, 794, 1148, 1942, 2111
HpySE526I	ACGT	2 cut(s)	311, 1614
Hsp92I	GRCGYC	4 cut(s)	12, 185, 215, 311
Hsp92II	CATG	1 cut(s)	2102
HspAI	GCGC	3 cut(s)	140, 185, 390
KasI	GGCGCC	1 cut(s)	184
KflI	GGGWCCC	1 cut(s)	1160
Kpn2I	TCCGGA	1 cut(s)	1012
KspI	CCGCGG	1 cut(s)	83
Kzo9I	GATC	3 cut(s)	73, 250, 1987
LmnI	GCTCC	5 cut(s)	389, 638, 679, 873, 1018
Lsp1109I	GCAGC	3 cut(s)	129, 270, 957
LweI	GCATC	4 cut(s)	401, 757, 801, 1702
MaeI	CTAG	4 cut(s)	891, 1179, 1923, 1991
MaeII	ACGT	2 cut(s)	311, 1614
MaeIII	GTNAC	5 cut(s)	68, 607, 1610, 1885, 2014
MalI	GATC	3 cut(s)	75, 252, 1989
MboI	GATC	3 cut(s)	73, 250, 1987
MboII	GAAGA	8 cut(s)	659, 709, 712, 1066, 1398, 1498, 1673, 2084
MflI	RGATCY	1 cut(s)	1987
MhlI	GDGCHC	3 cut(s)	210, 890, 1983
MluCI	AATT	7 cut(s)	374, 415, 1058, 1134, 1218, 1859, 1907
Mly113I	GGCGCC	1 cut(s)	185
MlyI	GAGTC	4 cut(s)	533, 869, 1458, 2096
MmeI	TCCRAC	5 cut(s)	33, 109, 260, 261, 1480
MroI	TCCGGA	1 cut(s)	1012
MroXI	GAANNNNTTC	1 cut(s)	1766
MseI	TTAA	4 cut(s)	96, 1061, 1533, 1964
MslI	CAYNNNNRTG	1 cut(s)	1149
MspA1I	CMGCKG	3 cut(s)	82, 970, 1826
MspI	CCGG	9 cut(s)	113, 164, 176, 369, 387, 490, 500, 602, 1013
MspR9I	CCNGG	1 cut(s)	1068
Mva1269I	GAATGC	1 cut(s)	900
MvaI	CCWGG	1 cut(s)	1068
MvnI	CGCG	2 cut(s)	41, 82
MwoI	GCNNNNNNNGC	4 cut(s)	151, 160, 673, 904
NarI	GGCGCC	1 cut(s)	185
NdeII	GATC	3 cut(s)	73, 250, 1987
NheI	GCTAGC	1 cut(s)	1178
NlaIII	CATG	1 cut(s)	2102
NlaIV	GGNNCC	6 cut(s)	26, 90, 186, 681, 1161, 1162
NmuCI	GTSAC	3 cut(s)	607, 1885, 2014
NotI	GCGGCCGC	1 cut(s)	596
NruI	TCGCGA	1 cut(s)	41
NspV	TTCGAA	2 cut(s)	404, 485
PcsI	WCGNNNNNNNCGW	3 cut(s)	233, 305, 401
PctI	GAATGC	1 cut(s)	900
PdmI	GAANNNNTTC	1 cut(s)	1766
PkrI	GCNGC	9 cut(s)	121, 144, 260, 597, 600, 906, 972, 1378, 1828
Ple19I	CGATCG	1 cut(s)	253
PleI	GAGTC	4 cut(s)	532, 868, 1457, 2096
PluTI	GGCGCC	1 cut(s)	188
PpsI	GAGTC	4 cut(s)	532, 868, 1457, 2096
PpuMI	RGGWCCY	1 cut(s)	1160
Psp124BI	GAGCTC	1 cut(s)	210
Psp5II	RGGWCCY	1 cut(s)	1160
Psp6I	CCWGG	1 cut(s)	1066
PspFI	CCCAGC	1 cut(s)	1253
PspGI	CCWGG	1 cut(s)	1066
PspN4I	GGNNCC	6 cut(s)	26, 90, 186, 681, 1161, 1162
PspPI	GGNCC	8 cut(s)	25, 56, 169, 492, 1160, 1318, 1645, 1751
PspPPI	RGGWCCY	1 cut(s)	1160
PstNI	CAGNNNCTG	1 cut(s)	2069
PsuI	RGATCY	1 cut(s)	1987
PvuI	CGATCG	1 cut(s)	253
PvuII	CAGCTG	1 cut(s)	970
RruI	TCGCGA	1 cut(s)	41
RsaI	GTAC	5 cut(s)	32, 50, 1525, 1600, 2121
RsaNI	GTAC	5 cut(s)	31, 49, 1524, 1599, 2120
RseI	CAYNNNNRTG	1 cut(s)	1149
SacI	GAGCTC	1 cut(s)	210
SacII	CCGCGG	1 cut(s)	83
SaqAI	TTAA	4 cut(s)	96, 1061, 1533, 1964
SatI	GCNGC	9 cut(s)	120, 143, 259, 596, 599, 905, 971, 1377, 1827
Sau3AI	GATC	3 cut(s)	73, 250, 1987
Sau96I	GGNCC	8 cut(s)	25, 56, 169, 492, 1160, 1318, 1645, 1751
ScaI	AGTACT	2 cut(s)	32, 1525
SchI	GAGTC	4 cut(s)	533, 869, 1458, 2096
ScrFI	CCNGG	1 cut(s)	1068
SduI	GDGCHC	3 cut(s)	210, 890, 1983
SfaNI	GCATC	4 cut(s)	401, 757, 801, 1702
SfcI	CTRYAG	1 cut(s)	712
SfoI	GGCGCC	1 cut(s)	186
Sfr303I	CCGCGG	1 cut(s)	83
SfuI	TTCGAA	2 cut(s)	404, 485
SgrAI	CRCCGGYG	1 cut(s)	601
SgrBI	CCGCGG	1 cut(s)	83
SinI	GGWCC	4 cut(s)	25, 56, 169, 1160
SmiMI	CAYNNNNRTG	1 cut(s)	1149
SmlI	CTYRAG	4 cut(s)	209, 899, 1640, 1784
SmoI	CTYRAG	4 cut(s)	209, 899, 1640, 1784
Sse9I	AATT	7 cut(s)	374, 415, 1058, 1134, 1218, 1859, 1907
SspDI	GGCGCC	1 cut(s)	184
SspMI	CTAG	4 cut(s)	891, 1179, 1923, 1991
SstI	GAGCTC	1 cut(s)	210
StyD4I	CCNGG	1 cut(s)	1066
StyI	CCWWGG	1 cut(s)	1754
TaaI	ACNGT	5 cut(s)	20, 56, 229, 301, 1301
TaiI	ACGT	2 cut(s)	314, 1617
TasI	AATT	7 cut(s)	374, 415, 1058, 1134, 1218, 1859, 1907
TatI	WGTACW	4 cut(s)	30, 48, 1523, 1598
TauI	GCSGC	6 cut(s)	122, 598, 601, 907, 1379, 1829
Tru1I	TTAA	4 cut(s)	96, 1061, 1533, 1964
Tru9I	TTAA	4 cut(s)	96, 1061, 1533, 1964
TscAI	CASTG	2 cut(s)	25, 597
TseFI	GTSAC	3 cut(s)	607, 1885, 2014
TseI	GCWGC	3 cut(s)	142, 258, 970
Tsp45I	GTSAC	3 cut(s)	607, 1885, 2014
TspDTI	ATGAA	6 cut(s)	552, 660, 953, 1293, 1920, 2010
TspGWI	ACGGA	4 cut(s)	232, 517, 536, 1734
TspRI	CASTG	2 cut(s)	25, 597
VpaK11BI	GGWCC	4 cut(s)	25, 56, 169, 1160
XbaI	TCTAGA	2 cut(s)	890, 1990
XcmI	CCANNNNNNNNNTGG	3 cut(s)	574, 1074, 1151
XmnI	GAANNNNTTC	1 cut(s)	1766
XspI	CTAG	4 cut(s)	891, 1179, 1923, 1991
ZraI	GACGTC	1 cut(s)	312
ZrmI	AGTACT	2 cut(s)	32, 1525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.