Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002708

Physical Location & Seq

Genomic Coordinates Forward (+)

14391470 .. 14396198

Gene Structure 4729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017231

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGCCGCCGGAGGCGGAGAAGGCGGAGGAGGGGGCGCAGTACTCGTTTGCTATGGAGTATAAAGGTCCTCCGGTGAGTTACGACCTTCCGAAGGCGGTTCCGATCAACGTCGACAGAATCCCCGTGGCGGCTGTCGTCGGACAGGTGTCTATTCCGGATAAGTTGTCGTTGCCGGTGGTCCAGCCGGTTCTGGCGCCCGGTTCGCTGATGAAAACGTTCTCCAAAGAGCTCAAGGCTTCCCAGTCGACGGTGTCTCCGACTTCGGTGATCGCTTTTGATCGGAGAAGTGAGGACGACACGACGAAGGAGTTGGAAGGGTCGGAATCGGCGACGGTTTCGCCGACGTCGGTGATTGGGTTCGAGGAGAGAGCTGAGGAGAGGGGAACTGGGTGTGGTGCTTTGTCCGGAGAATTGAGTAGCTCCGGCGGGTTGGAGTTTTCGAACGGGTTGAATTACGGGTCGGGTGAGCTGTCCGATGTGAATAACGGTAGTTGGGCTTGGAGCGGAGGAGCATTGACGGCTCCGAGTGGTGGGAAGGAGAGTTTGGATTTGAATAGCGATTTGAATCAACCCGAACCCGATTGGGCTTCTTCTGAGTCCGTTTTGAGCTTGGACTATCCGTCTTCGCGGGTTTCCTCCACCAAGGCTGTGGATTGCGATGTGAAACGACCCGCTGTGGTGACGTTTCGGGACATTGAATCTGATGATGATGTTGGTGAGGAAGATGAGGCTGAGGTTGTGCCGGTGAAGCGGGAGCCTCAGAGCAAAGGAAAGAAGAAGTCTTGTTATAGATGTCTGAAGGGGACTAGGTTCACTGAGAAGGAGGTGTGCATTGTTTGTGACGCCAAGTATTGTAGTAGTTGTGTTCTGAGAGCTATGGGGTCTATGCCGGAAGGCCGAAAGTGTGTTGCTTGTATTGGATTTCCTATTGATGAGAACAAAAGAGGGTCCTTGGGGAAGTGCTCTAGGATGCTGAAGCGGCTGCTGAATGATTTGGAGGTTCGGCAGGTGATGAAAGCCGAAAAGTTTTGTGAAGCAAATCAGCTACCGCCGAATTACATCTGTGTGAATGGACAGCCTCTTTGTCATGAGGAGCTTGTTATGTTACAGACCTGCTCAAACCCCCCGAAGAAGCTAAAACCAGGAAACTATTGGTATGATAAAGTTTCTGGACTTTGGGGAAAGGAAGGACAGAAGCCTTCGAAGGTAATCAGTCCCCATCTTAGTGTTGGGGGTCCCATCAAGTCAAATGCTAGTAATGGAAACACACAGGTTTTTATCAATGGCCGGGAAATTACCAAAGTTGAGCTTCGGATGTTACAGTTGGCAGGAGTTCAGTGTGCTGGTAACCCACACTTTTGGGTGAATGAGGATGGGTCATACCAAGAGGAGGGCCAGAAAAATACTAAAGGATACATATGGGGAAAGGCTGGAACCAAGCTTGTTTGTGCTGTCTTGTCACTGCCAGTGCCTTCTAAATCTTCGAATCCTTGTGGAGAACCCCTGAGCTATGTTGGTGGCAGAGTTGTCCCTGATTACATTGAGCAGAGAATACTTCAGAAAATTCTTTTAGTTGGATATAATGGATCCGGAACAAGTACTATATTCAAACAGGCCAAGATTCTATATAAAGCTATCCCTTTCTCGGAAGATGAGCGTGAAAATATCAAGTTTACAATACAGAGCAATGTATATGGCTATCTTGGTATACTACTGGAGGGACGTGAGCGTTTTGAAGAGGAAAGTTTGGCTGAGATTAGAAATCAATGTTCTTCTAGTCAAACAGATGCGAGAGGGAACAACGACAAAACTCTTTATTCCATTGGCCCGAGACTGAAAGCATTTTCTGATTGGCTTCTCAAGATAATGGTGTCAGGTGATTTGGAAGCCATTTTCCCAGCTGCTACCCGTGAATATGCACCATTGGTTGAGGAGTTGTGGAATGATTCAGCCATTCAAGCTACATTCAAGCGGAGAAGTGAATTGGAAATGTTACCTGGTGTTGCTACTTATTTCATAGAGAGGGCTGTTGACATATTAAGAGTAGACTATGAGCCCTCGGATCTGGATATTCTATACGCTGAAGGTGTTACTTCATCTAATGGGCTTGCTTGTGTAGACTTCTCATTTCCACTGTCGGCATCTGAAGATAACATCAATCATAATAATGATCAGCAGGACTCTTTGCTTAGGTACCAACTGATTAGAGTAAATGCAAGAGGACTAGGAGAAAACTGCAAATGGTTGGAGATGTTTGAAGATGTTGGAATGGTCATATTCTGTGTATCCTTGAGCGACTATGATCATTTTTCTGTGGATTGTAGTGGGTCCTTTTCAAACAAGATGATACAGACCAGAAGTTTCTTTGAAAGCATCATTACTCATCCAACATTTGAACATATGGACTTCCTTTTGATTCTAAACAAGTTTGATGTGCTTGAGGAAAAGGTAGAAAGGGTACCACTTACTCAGTGTGACTGGTTTGATGATTTCCATCCGGTGGTTAGCAGACATCGGTCCAGCAGCAACAACAGCAGCAACAACATTAATAGTAGCCCCTCGCTGGGTCATCTAGCAGCTTACTACATTGCAGTAAAGTTCAAGAGGCTATATTCTTCTCTAACCGGAAAGAAACTTTACGTTTCTCTGGTAAAAGGGTTGCAGCCTAATAGCGTAGATGCAGCTCTTAAATATTCAAGGGAGATTTTAAAATGGGATGAACAGAGAGGCAACTTCAGCTTTGATTACTCTGTCTATAGCACTGAGGCAAGCTCTTATTCACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009898 GO:0009987 GO:0016020 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0030234 GO:0030695 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0034260 GO:0035639 GO:0036094 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0048519 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051346 GO:0051716 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

929

Amino Acids

102.62

Weight (kDa)

5.26

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	520 - 901	9.2e-63	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23460
fragaria_vesca	FvH4_1g14590 FvH4_1g14590
malus_domestica	MD00G1067300.v1.1 MD02G1159500.v1.1
prunus_persica	Prupe.7G144400_v2.0.a1
pyrus_communis	pycom02g12580 pycom15g23720
rosa_chinensis	RchiOBHm_Chr1g0367271 RchiOBHm_Chr1g0367281 RchiOBHm_Chr1g0367331 RchiOBHm_Chr2g0103371
rosa_laevigata	RLG00000017231 RLG00000027279 RLG00000027284
rosa_multiflora	Rmu_sc0001555.1_g000003 Rmu_sc0002434.1_g000010 Rmu_sc0011000.1_g000013
rosa_roxburghii	Rroxscaffold_2G00139680 Rroxscaffold_4G00289390 Rroxscaffold_4G00289470 Rroxscaffold_4G00289480
rosa_rugosa	Rorug02G0114700 Rorug06G0002300
rosa_samantha	Rh1AG342600 Rh1BG303200 Rh1BG303600 Rh1BG303700 Rh1CG319500 Rh1CG319700 Rh1DG335600 Rh1DG336000 Rh1DG336100 Rh2AG162700 Rh2BG169800 Rh2CG169900 Rh2DG168800
rosa_wichuraiana	Rw1G030440 Rw2G012780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	997
AatII	GACGTC	1 cut(s)	347
Acc36I	ACCTGC	2 cut(s)	997, 1121
Acc65I	GGTACC	2 cut(s)	2193, 2458
AccB1I	GGYRCC	3 cut(s)	193, 2193, 2458
AccB7I	CCANNNNNTGG	1 cut(s)	2500
AccBSI	CCGCTC	1 cut(s)	504
AccI	GTMKAC	5 cut(s)	111, 245, 1708, 2046, 2118
AccII	CGCG	1 cut(s)	628
AccIII	TCCGGA	3 cut(s)	154, 404, 1589
AclI	AACGTT	1 cut(s)	215
AclWI	GGATC	3 cut(s)	1581, 1594, 2070
AcoI	YGGCCR	1 cut(s)	1285
AcsI	RAATTY	1 cut(s)	1563
AcuI	CTGAAG	6 cut(s)	818, 995, 1541, 2103, 2166, 2719
AcyI	GRCGYC	3 cut(s)	194, 344, 843
AfaI	GTAC	4 cut(s)	41, 1600, 2195, 2460
AfiI	CCNNNNNNNGG	9 cut(s)	91, 127, 247, 530, 1390, 1645, 2500, 2563, 2564
AhdI	GACNNNNNGTC	1 cut(s)	619
AjiI	CACGTC	1 cut(s)	1724
AjnI	CCWGG	2 cut(s)	1141, 1996
AjuI	GAANNNNNNNTTGG	2 cut(s)	527, 559
Alw21I	GWGCWC	2 cut(s)	231, 965
Alw26I	GTCTC	2 cut(s)	258, 1825
AlwI	GGATC	3 cut(s)	1581, 1594, 2070
Ama87I	CYCGRG	1 cut(s)	1828
Aor13HI	TCCGGA	3 cut(s)	154, 404, 1589
AoxI	GGCC	5 cut(s)	895, 1285, 1393, 1614, 1825
ApeKI	GCWGC	7 cut(s)	982, 1901, 2523, 2535, 2576, 2662, 2681
ApoI	RAATTY	1 cut(s)	1563
ArsI	GACNNNNNNTTYG	2 cut(s)	1065, 1097
AseI	ATTAAT	1 cut(s)	2547
Asp700I	GAANNNNTTC	1 cut(s)	215
Asp718I	GGTACC	2 cut(s)	2193, 2458
AspLEI	GCGC	2 cut(s)	37, 196
AspS9I	GGNCC	8 cut(s)	65, 178, 948, 1235, 1393, 1826, 2328, 2517
AsuC2I	CCSGG	2 cut(s)	198, 1289
AsuII	TTCGAA	3 cut(s)	440, 1202, 1484
AvaI	CYCGRG	1 cut(s)	1828
AvaII	GGWCC	6 cut(s)	65, 178, 948, 1235, 2328, 2517
BamHI	GGATCC	1 cut(s)	1586
BanI	GGYRCC	3 cut(s)	193, 2193, 2458
BanII	GRGCYC	2 cut(s)	231, 2058
BbsI	GAAGAC	1 cut(s)	615
Bbv12I	GWGCWC	2 cut(s)	231, 965
BbvCI	CCTCAGC	2 cut(s)	372, 732
BbvI	GCAGC	7 cut(s)	969, 1888, 2535, 2547, 2588, 2674, 2693
BccI	CCATC	4 cut(s)	1227, 1247, 1367, 2502
BceAI	ACGGC	1 cut(s)	534
BciT130I	CCWGG	2 cut(s)	1143, 1998
BciVI	GTATCC	2 cut(s)	1406, 2296
BclI	TGATCA	2 cut(s)	2170, 2302
BcnI	CCSGG	2 cut(s)	198, 1289
BcoDI	GTCTC	2 cut(s)	258, 1825
BfaI	CTAG	6 cut(s)	807, 966, 1254, 1776, 2225, 2573
BfmI	CTRYAG	1 cut(s)	2755
BfoI	RGCGCY	1 cut(s)	197
BfuAI	ACCTGC	2 cut(s)	997, 1121
BfuI	GTATCC	2 cut(s)	1406, 2296
BmcAI	AGTACT	2 cut(s)	41, 1600
Bme1390I	CCNGG	4 cut(s)	198, 1143, 1289, 1998
Bme18I	GGWCC	6 cut(s)	65, 178, 948, 1235, 2328, 2517
BmeRI	GACNNNNNGTC	1 cut(s)	619
BmeT110I	CYCGRG	1 cut(s)	1828
BmgBI	CACGTC	1 cut(s)	1724
BmgT120I	GGNCC	8 cut(s)	65, 178, 948, 1235, 1393, 1826, 2328, 2517
BmrFI	CCNGG	4 cut(s)	198, 1143, 1289, 1998
BmrI	ACTGGG	2 cut(s)	235, 396
BmsI	GCATC	5 cut(s)	960, 1777, 2150, 2382, 2668
BmuI	ACTGGG	2 cut(s)	235, 396
BoxI	GACNNNNGTC	2 cut(s)	145, 2515
BpiI	GAAGAC	1 cut(s)	615
BplI	GAGNNNNNCTC	1 cut(s)	2756
BpmI	CTGGAG	1 cut(s)	1736
Bpu10I	CCTNAGC	4 cut(s)	372, 732, 1505, 2189
Bpu14I	TTCGAA	3 cut(s)	440, 1202, 1484
BpuEI	CTTGAG	4 cut(s)	215, 1844, 2311, 2459
BpuMI	CCSGG	2 cut(s)	198, 1289
BsaBI	GATNNNNATC	2 cut(s)	564, 2493
BsaHI	GRCGYC	3 cut(s)	194, 344, 843
BsaJI	CCNNGG	4 cut(s)	123, 642, 951, 2058
BsaWI	WCCGGW	6 cut(s)	70, 154, 404, 1589, 2497, 2624
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	9 cut(s)	91, 127, 247, 530, 1390, 1645, 2500, 2563, 2564
Bse118I	RCCGGY	3 cut(s)	172, 184, 742
Bse1I	ACTGG	5 cut(s)	241, 391, 1466, 1719, 2483
Bse3DI	GCAATG	2 cut(s)	1693, 2586
Bse8I	GATNNNNATC	2 cut(s)	564, 2493
BseAI	TCCGGA	3 cut(s)	154, 404, 1589
BseBI	CCWGG	2 cut(s)	1143, 1998
BseDI	CCNNGG	4 cut(s)	123, 642, 951, 2058
BseGI	GGATG	6 cut(s)	975, 1320, 1378, 2383, 2494, 2722
BseJI	GATNNNNATC	2 cut(s)	564, 2493
BseLI	CCNNNNNNNGG	9 cut(s)	91, 127, 247, 530, 1390, 1645, 2500, 2563, 2564
BseMI	GCAATG	2 cut(s)	1693, 2586
BseNI	ACTGG	5 cut(s)	241, 391, 1466, 1719, 2483
BseRI	GAGGAG	7 cut(s)	41, 377, 389, 522, 1106, 1403, 1946
BseXI	GCAGC	7 cut(s)	969, 1888, 2535, 2547, 2588, 2674, 2693
BseYI	CCCAGC	2 cut(s)	1897, 2563
Bsh1236I	CGCG	1 cut(s)	628
BshFI	GGCC	5 cut(s)	897, 1287, 1395, 1616, 1827
BshNI	GGYRCC	3 cut(s)	193, 2193, 2458
BsiHKAI	GWGCWC	2 cut(s)	231, 965
BsiHKCI	CYCGRG	1 cut(s)	1828
BslFI	GGGAC	6 cut(s)	704, 817, 1200, 1221, 1514, 1734
BslI	CCNNNNNNNGG	9 cut(s)	91, 127, 247, 530, 1390, 1645, 2500, 2563, 2564
BsmAI	GTCTC	2 cut(s)	258, 1825
BsmFI	GGGAC	6 cut(s)	704, 817, 1200, 1221, 1514, 1734
BsnI	GGCC	5 cut(s)	897, 1287, 1395, 1616, 1827
BsoBI	CYCGRG	1 cut(s)	1828
Bsp119I	TTCGAA	3 cut(s)	440, 1202, 1484
Bsp1286I	GDGCHC	3 cut(s)	231, 965, 2058
Bsp13I	TCCGGA	3 cut(s)	154, 404, 1589
Bsp143I	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
BspANI	GGCC	5 cut(s)	897, 1287, 1395, 1616, 1827
BspEI	TCCGGA	3 cut(s)	154, 404, 1589
BspFNI	CGCG	1 cut(s)	628
BspHI	TCATGA	1 cut(s)	1087
BspMI	ACCTGC	2 cut(s)	997, 1121
BspPI	GGATC	3 cut(s)	1581, 1594, 2070
BspT104I	TTCGAA	3 cut(s)	440, 1202, 1484
BspT107I	GGYRCC	3 cut(s)	193, 2193, 2458
BsrBI	CCGCTC	1 cut(s)	504
BsrDI	GCAATG	2 cut(s)	1693, 2586
BsrFI	RCCGGY	3 cut(s)	172, 184, 742
BsrI	ACTGG	5 cut(s)	241, 391, 1466, 1719, 2483
BssAI	RCCGGY	3 cut(s)	172, 184, 742
BssECI	CCNNGG	4 cut(s)	123, 642, 951, 2058
BssMI	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
BssNAI	GTATAC	1 cut(s)	1709
BssNI	GRCGYC	3 cut(s)	194, 344, 843
BssT1I	CCWWGG	2 cut(s)	642, 951
Bst1107I	GTATAC	1 cut(s)	1709
Bst2UI	CCWGG	2 cut(s)	1143, 1998
Bst4CI	ACNGT	5 cut(s)	250, 334, 488, 1323, 2136
Bst6I	CTCTTC	1 cut(s)	1731
BstACI	GRCGYC	3 cut(s)	194, 344, 843
BstBI	TTCGAA	3 cut(s)	440, 1202, 1484
BstC8I	GCNNGC	2 cut(s)	2109, 2770
BstDSI	CCRYGG	1 cut(s)	123
BstEII	GGTNACC	1 cut(s)	1346
BstENI	CCTNNNNNAGG	1 cut(s)	89
BstF5I	GGATG	6 cut(s)	975, 1320, 1378, 2383, 2494, 2722
BstFNI	CGCG	1 cut(s)	628
BstH2I	RGCGCY	1 cut(s)	197
BstHHI	GCGC	2 cut(s)	37, 196
BstKTI	GATC	7 cut(s)	105, 270, 280, 1589, 2065, 2173, 2305
BstMAI	GTCTC	2 cut(s)	258, 1825
BstMBI	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
BstMWI	GCNNNNNNNGC	8 cut(s)	20, 202, 518, 748, 979, 1958, 2532, 2736
BstNI	CCWGG	2 cut(s)	1143, 1998
BstPAI	GACNNNNGTC	2 cut(s)	145, 2515
BstPI	GGTNACC	1 cut(s)	1346
BstSCI	CCNGG	4 cut(s)	196, 1141, 1287, 1996
BstSFI	CTRYAG	1 cut(s)	2755
BstUI	CGCG	1 cut(s)	628
BstV1I	GCAGC	7 cut(s)	969, 1888, 2535, 2547, 2588, 2674, 2693
BstV2I	GAAGAC	1 cut(s)	615
BstX2I	RGATCY	2 cut(s)	1586, 2062
BstXI	CCANNNNNNTGG	2 cut(s)	649, 1359
BstYI	RGATCY	2 cut(s)	1586, 2062
BstZ17I	GTATAC	1 cut(s)	1709
BsuI	GTATCC	2 cut(s)	1406, 2296
BsuRI	GGCC	5 cut(s)	897, 1287, 1395, 1616, 1827
BtgI	CCRYGG	1 cut(s)	123
BtgZI	GCGATG	1 cut(s)	672
BtrI	CACGTC	1 cut(s)	1724
BtsCI	GGATG	6 cut(s)	975, 1320, 1378, 2383, 2494, 2722
BtsI	GCAGTG	1 cut(s)	1460
BtsIMutI	CAGTG	7 cut(s)	813, 1343, 1460, 1473, 2132, 2477, 2760
BveI	ACCTGC	2 cut(s)	997, 1121
Cac8I	GCNNGC	2 cut(s)	2109, 2770
CciI	TCATGA	1 cut(s)	1087
CfoI	GCGC	2 cut(s)	37, 196
Cfr10I	RCCGGY	3 cut(s)	172, 184, 742
Cfr13I	GGNCC	8 cut(s)	65, 178, 948, 1235, 1393, 1826, 2328, 2517
CseI	GACGC	1 cut(s)	851
CsiI	ACCWGGT	1 cut(s)	1996
Csp6I	GTAC	4 cut(s)	40, 1599, 2194, 2459
CviAII	CATG	1 cut(s)	1088
CviQI	GTAC	4 cut(s)	40, 1599, 2194, 2459
DinI	GGCGCC	1 cut(s)	195
DpnI	GATC	7 cut(s)	104, 269, 279, 1588, 2064, 2172, 2304
DpnII	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
DraI	TTTAAA	1 cut(s)	2709
DriI	GACNNNNNGTC	1 cut(s)	619
EaeI	YGGCCR	1 cut(s)	1285
Eam1104I	CTCTTC	1 cut(s)	1731
Eam1105I	GACNNNNNGTC	1 cut(s)	619
EarI	CTCTTC	1 cut(s)	1731
EciI	GGCGGA	2 cut(s)	29, 38
Ecl136II	GAGCTC	1 cut(s)	229
Eco130I	CCWWGG	2 cut(s)	642, 951
Eco24I	GRGCYC	2 cut(s)	231, 2058
Eco47I	GGWCC	6 cut(s)	65, 178, 948, 1235, 2328, 2517
Eco53kI	GAGCTC	1 cut(s)	229
Eco57I	CTGAAG	6 cut(s)	818, 995, 1541, 2103, 2166, 2719
Eco88I	CYCGRG	1 cut(s)	1828
Eco91I	GGTNACC	1 cut(s)	1346
EcoICRI	GAGCTC	1 cut(s)	229
EcoNI	CCTNNNNNAGG	1 cut(s)	89
EcoO109I	RGGNCCY	4 cut(s)	65, 948, 1235, 2328
EcoO65I	GGTNACC	1 cut(s)	1346
EcoRII	CCWGG	2 cut(s)	1141, 1996
EcoT14I	CCWWGG	2 cut(s)	642, 951
EcoT38I	GRGCYC	2 cut(s)	231, 2058
EgeI	GGCGCC	1 cut(s)	195
EheI	GGCGCC	1 cut(s)	195
ErhI	CCWWGG	2 cut(s)	642, 951
FaeI	CATG	1 cut(s)	1091
FalI	AAGNNNNNCTT	2 cut(s)	1293, 1325
FaqI	GGGAC	6 cut(s)	704, 817, 1200, 1221, 1514, 1734
FatI	CATG	1 cut(s)	1087
FauI	CCCGC	4 cut(s)	419, 621, 679, 744
FauNDI	CATATG	2 cut(s)	1418, 2400
FbaI	TGATCA	2 cut(s)	2170, 2302
FblI	GTMKAC	5 cut(s)	111, 245, 1708, 2046, 2118
FokI	GGATG	6 cut(s)	982, 1327, 1385, 2370, 2481, 2729
FriOI	GRGCYC	2 cut(s)	231, 2058
FspBI	CTAG	6 cut(s)	807, 966, 1254, 1776, 2225, 2573
GlaI	GCGC	2 cut(s)	36, 195
GsaI	CCCAGC	2 cut(s)	1901, 2567
GsuI	CTGGAG	1 cut(s)	1736
HaeII	RGCGCY	1 cut(s)	197
HaeIII	GGCC	5 cut(s)	897, 1287, 1395, 1616, 1827
HgaI	GACGC	1 cut(s)	851
HhaI	GCGC	2 cut(s)	37, 196
Hin1I	GRCGYC	3 cut(s)	194, 344, 843
Hin1II	CATG	1 cut(s)	1091
Hin6I	GCGC	2 cut(s)	35, 194
HinP1I	GCGC	2 cut(s)	35, 194
HincII	GTYRAC	3 cut(s)	112, 246, 2032
HindII	GTYRAC	3 cut(s)	112, 246, 2032
HindIII	AAGCTT	1 cut(s)	1439
Hpy166II	GTNNAC	8 cut(s)	112, 246, 813, 1674, 1709, 2032, 2047, 2119
Hpy188III	TCNNGA	9 cut(s)	155, 405, 689, 1088, 1170, 1590, 1861, 2066, 2602
Hpy8I	GTNNAC	8 cut(s)	112, 246, 813, 1674, 1709, 2032, 2047, 2119
Hpy99I	CGWCG	7 cut(s)	113, 140, 250, 304, 334, 346, 349
HpyCH4III	ACNGT	5 cut(s)	250, 334, 488, 1323, 2136
HpyCH4IV	ACGT	6 cut(s)	108, 215, 344, 683, 1723, 2640
HpyCH4V	TGCA	7 cut(s)	831, 1919, 2216, 2238, 2591, 2662, 2681
HpyF10VI	GCNNNNNNNGC	8 cut(s)	20, 202, 518, 748, 979, 1958, 2532, 2736
HpySE526I	ACGT	6 cut(s)	108, 215, 344, 683, 1723, 2640
Hsp92I	GRCGYC	3 cut(s)	194, 344, 843
Hsp92II	CATG	1 cut(s)	1091
HspAI	GCGC	2 cut(s)	35, 194
KasI	GGCGCC	1 cut(s)	193
KflI	GGGWCCC	1 cut(s)	1235
Kpn2I	TCCGGA	3 cut(s)	154, 404, 1589
KpnI	GGTACC	2 cut(s)	2197, 2462
Ksp22I	TGATCA	2 cut(s)	2170, 2302
Kzo9I	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
LmnI	GCTCC	6 cut(s)	425, 501, 509, 526, 754, 1093
Lsp1109I	GCAGC	7 cut(s)	969, 1888, 2535, 2547, 2588, 2674, 2693
LweI	GCATC	5 cut(s)	960, 1777, 2150, 2382, 2668
MabI	ACCWGGT	1 cut(s)	1996
MaeI	CTAG	6 cut(s)	807, 966, 1254, 1776, 2225, 2573
MaeII	ACGT	6 cut(s)	108, 215, 344, 683, 1723, 2640
MalI	GATC	7 cut(s)	104, 269, 279, 1588, 2064, 2172, 2304
MbiI	CCGCTC	1 cut(s)	504
MboI	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
MflI	RGATCY	2 cut(s)	1586, 2062
MhlI	GDGCHC	3 cut(s)	231, 965, 2058
MluCI	AATT	6 cut(s)	410, 451, 1054, 1293, 1563, 1982
Mly113I	GGCGCC	1 cut(s)	194
MlyI	GAGTC	2 cut(s)	605, 2174
MmeI	TCCRAC	9 cut(s)	118, 281, 291, 300, 411, 1555, 2226, 2245, 2411
MroI	TCCGGA	3 cut(s)	154, 404, 1589
MroXI	GAANNNNTTC	1 cut(s)	215
MseI	TTAA	4 cut(s)	2039, 2547, 2688, 2708
MslI	CAYNNNNRTG	3 cut(s)	1064, 1224, 2166
MspA1I	CMGCKG	2 cut(s)	674, 1901
MspR9I	CCNGG	4 cut(s)	198, 1143, 1289, 1998
MvaI	CCWGG	2 cut(s)	1143, 1998
MvnI	CGCG	1 cut(s)	628
MwoI	GCNNNNNNNGC	8 cut(s)	20, 202, 518, 748, 979, 1958, 2532, 2736
NarI	GGCGCC	1 cut(s)	194
NciI	CCSGG	2 cut(s)	198, 1289
NdeI	CATATG	2 cut(s)	1418, 2400
NdeII	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
NlaIII	CATG	1 cut(s)	1091
NmuCI	GTSAC	4 cut(s)	679, 839, 1458, 2474
NspV	TTCGAA	3 cut(s)	440, 1202, 1484
PagI	TCATGA	1 cut(s)	1087
PaqCI	CACCTGC	1 cut(s)	997
PcsI	WCGNNNNNNNCGW	3 cut(s)	254, 326, 338
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	8 cut(s)	117, 323, 565, 698, 1486, 1621, 1946, 2416
PflFI	GACNNNGTC	1 cut(s)	250
PflMI	CCANNNNNTGG	1 cut(s)	2500
PleI	GAGTC	2 cut(s)	604, 2174
PluTI	GGCGCC	1 cut(s)	197
PpsI	GAGTC	2 cut(s)	604, 2174
PpuMI	RGGWCCY	4 cut(s)	65, 948, 1235, 2328
PshAI	GACNNNNGTC	2 cut(s)	145, 2515
PshBI	ATTAAT	1 cut(s)	2547
Psp124BI	GAGCTC	1 cut(s)	231
Psp1406I	AACGTT	1 cut(s)	215
Psp5II	RGGWCCY	4 cut(s)	65, 948, 1235, 2328
Psp6I	CCWGG	2 cut(s)	1141, 1996
PspEI	GGTNACC	1 cut(s)	1346
PspFI	CCCAGC	2 cut(s)	1897, 2563
PspGI	CCWGG	2 cut(s)	1141, 1996
PspPI	GGNCC	8 cut(s)	65, 178, 948, 1235, 1393, 1826, 2328, 2517
PspPPI	RGGWCCY	4 cut(s)	65, 948, 1235, 2328
PsuI	RGATCY	2 cut(s)	1586, 2062
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	1 cut(s)	1901
RsaI	GTAC	4 cut(s)	41, 1600, 2195, 2460
RsaNI	GTAC	4 cut(s)	40, 1599, 2194, 2459
RseI	CAYNNNNRTG	3 cut(s)	1064, 1224, 2166
SacI	GAGCTC	1 cut(s)	231
SalI	GTCGAC	2 cut(s)	110, 244
SaqAI	TTAA	4 cut(s)	2039, 2547, 2688, 2708
Sau3AI	GATC	7 cut(s)	102, 267, 277, 1586, 2062, 2170, 2302
Sau96I	GGNCC	8 cut(s)	65, 178, 948, 1235, 1393, 1826, 2328, 2517
ScaI	AGTACT	2 cut(s)	41, 1600
SchI	GAGTC	2 cut(s)	605, 2174
ScrFI	CCNGG	4 cut(s)	198, 1143, 1289, 1998
SduI	GDGCHC	3 cut(s)	231, 965, 2058
SexAI	ACCWGGT	1 cut(s)	1996
SfaNI	GCATC	5 cut(s)	960, 1777, 2150, 2382, 2668
SfcI	CTRYAG	1 cut(s)	2755
SfoI	GGCGCC	1 cut(s)	195
SfuI	TTCGAA	3 cut(s)	440, 1202, 1484
SinI	GGWCC	6 cut(s)	65, 178, 948, 1235, 2328, 2517
SmiMI	CAYNNNNRTG	3 cut(s)	1064, 1224, 2166
SmlI	CTYRAG	4 cut(s)	230, 1859, 2290, 2438
SmoI	CTYRAG	4 cut(s)	230, 1859, 2290, 2438
Sse9I	AATT	6 cut(s)	410, 451, 1054, 1293, 1563, 1982
SspDI	GGCGCC	1 cut(s)	193
SspI	AATATT	1 cut(s)	2693
SspMI	CTAG	6 cut(s)	807, 966, 1254, 1776, 2225, 2573
SstI	GAGCTC	1 cut(s)	231
StyD4I	CCNGG	4 cut(s)	196, 1141, 1287, 1996
StyI	CCWWGG	2 cut(s)	642, 951
TaaI	ACNGT	5 cut(s)	250, 334, 488, 1323, 2136
TaiI	ACGT	6 cut(s)	111, 218, 347, 686, 1726, 2643
TaqI	TCGA	6 cut(s)	111, 245, 360, 440, 1202, 1484
TaqII	GACCGA	1 cut(s)	2505
TasI	AATT	6 cut(s)	410, 451, 1054, 1293, 1563, 1982
TatI	WGTACW	2 cut(s)	39, 1598
TauI	GCSGC	3 cut(s)	7, 131, 982
TfiI	GAWTC	8 cut(s)	117, 323, 565, 698, 1486, 1621, 1946, 2416
Tru1I	TTAA	4 cut(s)	2039, 2547, 2688, 2708
Tru9I	TTAA	4 cut(s)	2039, 2547, 2688, 2708
TscAI	CASTG	7 cut(s)	820, 1343, 1467, 1473, 2139, 2477, 2767
TseFI	GTSAC	4 cut(s)	679, 839, 1458, 2474
TseI	GCWGC	7 cut(s)	982, 1901, 2523, 2535, 2576, 2662, 2681
Tsp45I	GTSAC	4 cut(s)	679, 839, 1458, 2474
TspDTI	ATGAA	5 cut(s)	224, 1028, 2005, 2085, 2733
TspGWI	ACGGA	2 cut(s)	589, 609
TspRI	CASTG	7 cut(s)	820, 1343, 1467, 1473, 2139, 2477, 2767
Tth111I	GACNNNGTC	1 cut(s)	250
Van91I	CCANNNNNTGG	1 cut(s)	2500
VpaK11BI	GGWCC	6 cut(s)	65, 178, 948, 1235, 2328, 2517
VspI	ATTAAT	1 cut(s)	2547
XagI	CCTNNNNNAGG	1 cut(s)	89
XapI	RAATTY	1 cut(s)	1563
XcmI	CCANNNNNNNNNTGG	3 cut(s)	646, 1149, 1226
XmiI	GTMKAC	5 cut(s)	111, 245, 1708, 2046, 2118
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	6 cut(s)	807, 966, 1254, 1776, 2225, 2573
ZraI	GACGTC	1 cut(s)	345
ZrmI	AGTACT	2 cut(s)	41, 1600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.