Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002708

Physical Location & Seq

Genomic Coordinates Reverse (-)

10022061 .. 10033315

Gene Structure 11255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00289390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGACTAGTGGTGGGATTCGAACAAGCGTGAAGGCCTACATTAATGAGCAGCGACTCGCTTCTTTGGCTGTGAATCATGAGAACAGTGAGGGCATCAATACCCATTTGGCTCCACTTGCAGAGGCTACCATGGCAGCAGCTTCAAGTAATTCATATGGGCTCTGTGATGGTAACATGCCTTGCTTGCCAATCTTGAGCTCTGCTACAAAGTCTTGTGAAGCAAATCAGCTGCCCCCGGCAGCGGATACTATTAGTGTGAATGGACAACCTCTTACTCCTGAAGAGCTTCTCACGTTACAAAGCTGCCCAAACCCTCCAAAGAAGCTAAAACCAGGAAACTATTGGTATGACAAGATTTGTGGACTTTGGGGGAAGGAAGGACAGAAGCCTTCAGAGGTAATCTCTCCCCACCTTTGTGTTGGGGGTCGCATGAAGGAAAATGCTAGCAACGGAAGCACACGGGTTTTCATTAATGGTCGGGAAATCACCAAAGTAGAGCTCCGGATGTTGCAGCTGGTAGGAGTTGAATGTACTGGTAACCCACACTTTTGGGTGACTGAAGATGGGTCATACCAAGAGGAGGGCATGAACAATGTTAAAGGATCCATATGGCGCATGCCACAATGCCACATGAGAAAGCTAGAAATGGAGCTGTCATCCAAGTTGTATGTTGGTATACTTCTTGAGGTTCGGGAACGTTTTGAAGAGGAATGTTTGGCTGAGATGAGGAGGCAATGCTCTAGTCAACCAGTTGCCAGTGGAGACAGCAACAAAACGCCCTACTCCATTGGCCCGAAGCTGAAAGCATTCTCTGATTGGCTTCTTAAGACTGTGGTGTCAGGCTATGTAGAGGCCATCTTCCCAGCTGCTACGCGTGAATTTGCACCATTTGTGGAGGAATTGTGGAATGATTCAGCCATTCAGGCCACATACAAGCGGTCAACTGAACTGGGAATGCTACCTAGTGTTGCTACTTATTTCATAAAGAGGGCTGTTCACATATTAAGAGTAAACTATGAGCCCACAGATGGAGACATCCTATATGCCGAAGTTGATACTTCATCTACTGGGTTGGCTTGTGTGGACTTCTCATTGCCCCAGTCAGTAATGAGAGGTGACTTTTATGGTCGTGATACTACTGATCAGCATGACTCTTTGCTTAGGTACCAACTTGTTAGAGTAAATGCAGGACTTGGAGAAAGCTGGGAACAGTTGGACATGTTTGGAGATGTTGGAATAGTCATCTTCTGTGTGTCCTTGAGTGACTATGATCAATTTTCTGCTGACGGTAATGGGACTTTCACAAACAAGATGCTGCAGAGCAGATCTGTTTTTGAGAGAATCGTTACTCATCCCACCTTTGAGCAGATGAACTGCCTTTTGCTTCTCAACGAGTTTGATTTGTTTGTGAAAAAGATGGCACGGGTCCCTTTAACTCGATGCGAATGGTTTGATGATTTTAATCCAGTGATTAACAATGACCTGGGTCATCGAGCATATGAGTATATAGCAGTCAAGTTCAAGAGGCTCAATTCTTCTCTAACTGACAAGAGATTGTATGTTTCGCTGGTGGAGGACTTTGAACCTGGTAATGTACGTGAAGCTTTAAAGTATTCAACGGAGATTTTAAATTGGGATGAAGTGAGAGACGATTATTGGTGTTATTCAGATTCAGATTACTCTATTTCAGATACAGATTCAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009898 GO:0009987 GO:0016020 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0030234 GO:0030695 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0034260 GO:0035639 GO:0036094 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0048519 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051346 GO:0051716 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

568

Amino Acids

63.85

Weight (kDa)

4.97

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	285 - 541	1.6e-44	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23460
fragaria_vesca	FvH4_1g14590 FvH4_1g14590
malus_domestica	MD00G1067300.v1.1 MD02G1159500.v1.1
prunus_persica	Prupe.7G144400_v2.0.a1
pyrus_communis	pycom02g12580 pycom15g23720
rosa_chinensis	RchiOBHm_Chr1g0367271 RchiOBHm_Chr1g0367281 RchiOBHm_Chr1g0367331 RchiOBHm_Chr2g0103371
rosa_laevigata	RLG00000017231 RLG00000027279 RLG00000027284
rosa_multiflora	Rmu_sc0001555.1_g000003 Rmu_sc0002434.1_g000010 Rmu_sc0011000.1_g000013
rosa_roxburghii	Rroxscaffold_2G00139680 Rroxscaffold_4G00289390 Rroxscaffold_4G00289470 Rroxscaffold_4G00289480
rosa_rugosa	Rorug02G0114700 Rorug06G0002300
rosa_samantha	Rh1AG342600 Rh1BG303200 Rh1BG303600 Rh1BG303700 Rh1CG319500 Rh1CG319700 Rh1DG335600 Rh1DG336000 Rh1DG336100 Rh2AG162700 Rh2BG169800 Rh2CG169900 Rh2DG168800
rosa_wichuraiana	Rw1G030440 Rw2G012780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	835
Acc65I	GGTACC	1 cut(s)	1164
AccB1I	GGYRCC	1 cut(s)	1164
AccI	GTMKAC	1 cut(s)	676
AccII	CGCG	1 cut(s)	874
AccIII	TCCGGA	1 cut(s)	501
AciI	CCGC	2 cut(s)	242, 937
AclI	AACGTT	1 cut(s)	697
AclWI	GGATC	2 cut(s)	597, 610
AcsI	RAATTY	1 cut(s)	878
AcuI	CTGAAG	3 cut(s)	300, 375, 579
AfaI	GTAC	3 cut(s)	532, 1166, 1596
AfiI	CCNNNNNNNGG	3 cut(s)	241, 580, 1028
AflII	CTTAAG	1 cut(s)	824
AflIII	ACRYGT	2 cut(s)	872, 1218
AgsI	TTSAA	6 cut(s)	144, 527, 704, 1522, 1583, 1617
AhlI	ACTAGT	1 cut(s)	5
AjnI	CCWGG	3 cut(s)	331, 1482, 1585
AleI	CACNNNNGTG	1 cut(s)	414
Alw21I	GWGCWC	2 cut(s)	200, 501
Alw26I	GTCTC	3 cut(s)	756, 1026, 1641
AlwI	GGATC	2 cut(s)	597, 610
Aor13HI	TCCGGA	1 cut(s)	501
AoxI	GGCC	4 cut(s)	33, 790, 852, 924
ApeKI	GCWGC	9 cut(s)	49, 134, 137, 229, 239, 303, 511, 866, 1315
ApoI	RAATTY	1 cut(s)	878
AseI	ATTAAT	2 cut(s)	42, 471
Asp700I	GAANNNNTTC	2 cut(s)	285, 806
Asp718I	GGTACC	1 cut(s)	1164
AspLEI	GCGC	1 cut(s)	615
AspS9I	GGNCC	2 cut(s)	791, 1426
AsuC2I	CCSGG	1 cut(s)	236
AsuHPI	GGTGA	3 cut(s)	478, 565, 1127
AsuII	TTCGAA	1 cut(s)	19
AsuNHI	GCTAGC	1 cut(s)	443
AvaII	GGWCC	1 cut(s)	1426
BamHI	GGATCC	1 cut(s)	602
BanI	GGYRCC	1 cut(s)	1164
BanII	GRGCYC	4 cut(s)	162, 200, 501, 1023
Bbv12I	GWGCWC	2 cut(s)	200, 501
BbvI	GCAGC	9 cut(s)	61, 146, 149, 216, 251, 290, 523, 853, 1302
BccI	CCATC	5 cut(s)	161, 557, 863, 1022, 1411
BciT130I	CCWGG	3 cut(s)	333, 1484, 1587
BciVI	GTATCC	1 cut(s)	238
BclI	TGATCA	2 cut(s)	1141, 1270
BcnI	CCSGG	1 cut(s)	236
BcoDI	GTCTC	3 cut(s)	756, 1026, 1641
BcuI	ACTAGT	1 cut(s)	5
BfaI	CTAG	5 cut(s)	6, 444, 641, 741, 963
BfmI	CTRYAG	1 cut(s)	1316
BfrI	CTTAAG	1 cut(s)	824
BfuI	GTATCC	1 cut(s)	238
BglI	GCCNNNNNGGC	1 cut(s)	923
BglII	AGATCT	1 cut(s)	1325
BisI	GCNGC	9 cut(s)	50, 135, 138, 230, 240, 304, 512, 867, 1316
BlsI	GCNGC	9 cut(s)	51, 136, 139, 231, 241, 305, 513, 868, 1317
Bme1390I	CCNGG	4 cut(s)	236, 333, 1484, 1587
Bme18I	GGWCC	1 cut(s)	1426
BmgT120I	GGNCC	2 cut(s)	791, 1426
BmiI	GGNNCC	5 cut(s)	111, 604, 1166, 1427, 1428
BmrFI	CCNGG	4 cut(s)	236, 333, 1484, 1587
BmrI	ACTGGG	3 cut(s)	959, 1077, 1093
BmsI	GCATC	3 cut(s)	102, 1302, 1430
BmtI	GCTAGC	1 cut(s)	447
BmuI	ACTGGG	3 cut(s)	959, 1077, 1093
BoxI	GACNNNNGTC	1 cut(s)	1485
BplI	GAGNNNNNCTC	2 cut(s)	386, 418
Bpu10I	CCTNAGC	1 cut(s)	1160
Bpu14I	TTCGAA	1 cut(s)	19
BpuEI	CTTGAG	3 cut(s)	214, 704, 1279
BpuMI	CCSGG	1 cut(s)	236
BsaAI	YACGTR	1 cut(s)	1598
BsaBI	GATNNNNATC	1 cut(s)	1461
BsaJI	CCNNGG	3 cut(s)	129, 234, 1483
BsaWI	WCCGGW	1 cut(s)	501
BsaXI	ACNNNNNCTCC	2 cut(s)	887, 917
Bsc4I	CCNNNNNNNGG	3 cut(s)	241, 580, 1028
Bse1I	ACTGG	7 cut(s)	538, 749, 756, 954, 1072, 1099, 1466
Bse3DI	GCAATG	2 cut(s)	740, 1091
Bse8I	GATNNNNATC	1 cut(s)	1461
BseAI	TCCGGA	1 cut(s)	501
BseBI	CCWGG	3 cut(s)	333, 1484, 1587
BseDI	CCNNGG	3 cut(s)	129, 234, 1483
BseGI	GGATG	5 cut(s)	510, 656, 1035, 1351, 1642
BseJI	GATNNNNATC	1 cut(s)	1461
BseLI	CCNNNNNNNGG	3 cut(s)	241, 580, 1028
BseMI	GCAATG	2 cut(s)	740, 1091
BseMII	CTCAG	1 cut(s)	711
BseNI	ACTGG	7 cut(s)	538, 749, 756, 954, 1072, 1099, 1466
BseRI	GAGGAG	2 cut(s)	593, 742
BseXI	GCAGC	9 cut(s)	61, 146, 149, 216, 251, 290, 523, 853, 1302
BseYI	CCCAGC	2 cut(s)	862, 1203
Bsh1236I	CGCG	1 cut(s)	874
BshFI	GGCC	4 cut(s)	35, 792, 854, 926
BshNI	GGYRCC	1 cut(s)	1164
BsiHKAI	GWGCWC	2 cut(s)	200, 501
BsiSI	CCGG	2 cut(s)	236, 502
BslFI	GGGAC	2 cut(s)	1309, 1412
BslI	CCNNNNNNNGG	3 cut(s)	241, 580, 1028
BsmAI	GTCTC	3 cut(s)	756, 1026, 1641
BsmBI	CGTCTC	1 cut(s)	1641
BsmFI	GGGAC	2 cut(s)	1309, 1412
BsmI	GAATGC	2 cut(s)	806, 960
BsnI	GGCC	4 cut(s)	35, 792, 854, 926
Bsp119I	TTCGAA	1 cut(s)	19
Bsp1286I	GDGCHC	4 cut(s)	162, 200, 501, 1023
Bsp13I	TCCGGA	1 cut(s)	501
Bsp143I	GATC	4 cut(s)	602, 1141, 1270, 1325
Bsp19I	CCATGG	1 cut(s)	129
BspACI	CCGC	2 cut(s)	242, 937
BspANI	GGCC	4 cut(s)	35, 792, 854, 926
BspCNI	CTCAG	1 cut(s)	712
BspEI	TCCGGA	1 cut(s)	501
BspFNI	CGCG	1 cut(s)	874
BspHI	TCATGA	1 cut(s)	76
BspLI	GGNNCC	5 cut(s)	111, 604, 1166, 1427, 1428
BspMAI	CTGCAG	1 cut(s)	1320
BspOI	GCTAGC	1 cut(s)	447
BspPI	GGATC	2 cut(s)	597, 610
BspQI	GCTCTTC	1 cut(s)	276
BspT104I	TTCGAA	1 cut(s)	19
BspT107I	GGYRCC	1 cut(s)	1164
BspTI	CTTAAG	1 cut(s)	824
BsrDI	GCAATG	2 cut(s)	740, 1091
BsrI	ACTGG	7 cut(s)	538, 749, 756, 954, 1072, 1099, 1466
BssECI	CCNNGG	3 cut(s)	129, 234, 1483
BssMI	GATC	4 cut(s)	602, 1141, 1270, 1325
BssNAI	GTATAC	1 cut(s)	677
BssT1I	CCWWGG	1 cut(s)	129
Bst1107I	GTATAC	1 cut(s)	677
Bst2UI	CCWGG	3 cut(s)	333, 1484, 1587
Bst4CI	ACNGT	4 cut(s)	86, 832, 1212, 1289
Bst6I	CTCTTC	2 cut(s)	276, 699
BstAFI	CTTAAG	1 cut(s)	824
BstBAI	YACGTR	1 cut(s)	1598
BstBI	TTCGAA	1 cut(s)	19
BstC8I	GCNNGC	3 cut(s)	185, 445, 617
BstDEI	CTNAG	2 cut(s)	720, 1160
BstDSI	CCRYGG	1 cut(s)	129
BstEII	GGTNACC	1 cut(s)	536
BstF5I	GGATG	5 cut(s)	510, 656, 1035, 1351, 1642
BstFNI	CGCG	1 cut(s)	874
BstHHI	GCGC	1 cut(s)	615
BstKTI	GATC	4 cut(s)	605, 1144, 1273, 1328
BstMAI	GTCTC	3 cut(s)	756, 1026, 1641
BstMBI	GATC	4 cut(s)	602, 1141, 1270, 1325
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 116, 131, 184, 453, 923
BstNI	CCWGG	3 cut(s)	333, 1484, 1587
BstNSI	RCATGY	3 cut(s)	178, 619, 1222
BstPAI	GACNNNNGTC	1 cut(s)	1485
BstPI	GGTNACC	1 cut(s)	536
BstSCI	CCNGG	4 cut(s)	234, 331, 1482, 1585
BstSFI	CTRYAG	1 cut(s)	1316
BstUI	CGCG	1 cut(s)	874
BstV1I	GCAGC	9 cut(s)	61, 146, 149, 216, 251, 290, 523, 853, 1302
BstX2I	RGATCY	2 cut(s)	602, 1325
BstXI	CCANNNNNNTGG	1 cut(s)	549
BstYI	RGATCY	2 cut(s)	602, 1325
BstZ17I	GTATAC	1 cut(s)	677
BsuI	GTATCC	1 cut(s)	238
BsuRI	GGCC	4 cut(s)	35, 792, 854, 926
BtgI	CCRYGG	1 cut(s)	129
BtsCI	GGATG	5 cut(s)	510, 656, 1035, 1351, 1642
BtsIMutI	CAGTG	3 cut(s)	91, 763, 1473
Cac8I	GCNNGC	3 cut(s)	185, 445, 617
CciI	TCATGA	1 cut(s)	76
CfoI	GCGC	1 cut(s)	615
Cfr13I	GGNCC	2 cut(s)	791, 1426
CsiI	ACCWGGT	1 cut(s)	1585
Csp6I	GTAC	3 cut(s)	531, 1165, 1595
CspCI	CAANNNNNGTGG	2 cut(s)	340, 375
CviAII	CATG	9 cut(s)	77, 130, 175, 430, 586, 616, 631, 1148, 1219
CviQI	GTAC	3 cut(s)	531, 1165, 1595
DdeI	CTNAG	2 cut(s)	720, 1160
DpnI	GATC	4 cut(s)	604, 1143, 1272, 1327
DpnII	GATC	4 cut(s)	602, 1141, 1270, 1325
DraI	TTTAAA	2 cut(s)	1608, 1629
DrdI	GACNNNNNNGTC	1 cut(s)	835
DseDI	GACNNNNNNGTC	1 cut(s)	835
Eam1104I	CTCTTC	2 cut(s)	276, 699
EarI	CTCTTC	2 cut(s)	276, 699
Ecl136II	GAGCTC	2 cut(s)	198, 499
Eco130I	CCWWGG	1 cut(s)	129
Eco147I	AGGCCT	1 cut(s)	35
Eco24I	GRGCYC	4 cut(s)	162, 200, 501, 1023
Eco47I	GGWCC	1 cut(s)	1426
Eco53kI	GAGCTC	2 cut(s)	198, 499
Eco57I	CTGAAG	3 cut(s)	300, 375, 579
Eco91I	GGTNACC	1 cut(s)	536
EcoICRI	GAGCTC	2 cut(s)	198, 499
EcoO109I	RGGNCCY	1 cut(s)	1426
EcoO65I	GGTNACC	1 cut(s)	536
EcoRII	CCWGG	3 cut(s)	331, 1482, 1585
EcoT14I	CCWWGG	1 cut(s)	129
EcoT38I	GRGCYC	4 cut(s)	162, 200, 501, 1023
ErhI	CCWWGG	1 cut(s)	129
Esp3I	CGTCTC	1 cut(s)	1641
FaeI	CATG	9 cut(s)	80, 133, 178, 433, 589, 619, 634, 1151, 1222
FaqI	GGGAC	2 cut(s)	1309, 1412
FatI	CATG	9 cut(s)	76, 129, 174, 429, 585, 615, 630, 1147, 1218
FauNDI	CATATG	3 cut(s)	154, 608, 1498
FbaI	TGATCA	2 cut(s)	1141, 1270
FblI	GTMKAC	1 cut(s)	676
Fnu4HI	GCNGC	9 cut(s)	50, 135, 138, 230, 240, 304, 512, 867, 1316
FokI	GGATG	5 cut(s)	517, 643, 1022, 1338, 1649
FriOI	GRGCYC	4 cut(s)	162, 200, 501, 1023
Fsp4HI	GCNGC	9 cut(s)	50, 135, 138, 230, 240, 304, 512, 867, 1316
FspBI	CTAG	5 cut(s)	6, 444, 641, 741, 963
GlaI	GCGC	1 cut(s)	614
GluI	GCNGC	9 cut(s)	50, 135, 138, 230, 240, 304, 512, 867, 1316
GsaI	CCCAGC	2 cut(s)	866, 1207
HaeIII	GGCC	4 cut(s)	35, 792, 854, 926
HapII	CCGG	2 cut(s)	236, 502
HhaI	GCGC	1 cut(s)	615
Hin1II	CATG	9 cut(s)	80, 133, 178, 433, 589, 619, 634, 1151, 1222
Hin6I	GCGC	1 cut(s)	613
HinP1I	GCGC	1 cut(s)	613
HincII	GTYRAC	2 cut(s)	746, 942
HindII	GTYRAC	2 cut(s)	746, 942
HindIII	AAGCTT	1 cut(s)	1602
HinfI	GANTC	8 cut(s)	16, 54, 73, 911, 1151, 1341, 1670, 1697
HpaII	CCGG	2 cut(s)	236, 502
HphI	GGTGA	3 cut(s)	478, 565, 1127
Hpy166II	GTNNAC	7 cut(s)	362, 677, 746, 942, 997, 1012, 1084
Hpy188I	TCNGA	6 cut(s)	394, 814, 1669, 1675, 1690, 1702
Hpy188III	TCNNGA	9 cut(s)	77, 193, 278, 479, 502, 683, 692, 1130, 1522
Hpy8I	GTNNAC	7 cut(s)	362, 677, 746, 942, 997, 1012, 1084
HpyAV	CCTTC	5 cut(s)	25, 367, 371, 399, 427
HpyCH4III	ACNGT	4 cut(s)	86, 832, 1212, 1289
HpyCH4IV	ACGT	3 cut(s)	293, 697, 1597
HpyCH4V	TGCA	5 cut(s)	119, 511, 884, 1187, 1318
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 116, 131, 184, 453, 923
HpyF3I	CTNAG	2 cut(s)	720, 1160
HpySE526I	ACGT	3 cut(s)	293, 697, 1597
Hsp92II	CATG	9 cut(s)	80, 133, 178, 433, 589, 619, 634, 1151, 1222
HspAI	GCGC	1 cut(s)	613
KflI	GGGWCCC	1 cut(s)	1426
Kpn2I	TCCGGA	1 cut(s)	501
KpnI	GGTACC	1 cut(s)	1168
Ksp22I	TGATCA	2 cut(s)	1141, 1270
Kzo9I	GATC	4 cut(s)	602, 1141, 1270, 1325
LguI	GCTCTTC	1 cut(s)	276
LmnI	GCTCC	3 cut(s)	115, 504, 649
Lsp1109I	GCAGC	9 cut(s)	61, 146, 149, 216, 251, 290, 523, 853, 1302
LweI	GCATC	3 cut(s)	102, 1302, 1430
MabI	ACCWGGT	1 cut(s)	1585
MaeI	CTAG	5 cut(s)	6, 444, 641, 741, 963
MaeII	ACGT	3 cut(s)	293, 697, 1597
MaeIII	GTNAC	7 cut(s)	170, 294, 536, 553, 1115, 1262, 1345
MalI	GATC	4 cut(s)	604, 1143, 1272, 1327
MboI	GATC	4 cut(s)	602, 1141, 1270, 1325
MboII	GAAGA	6 cut(s)	293, 572, 716, 850, 1237, 1527
MflI	RGATCY	2 cut(s)	602, 1325
MhlI	GDGCHC	4 cut(s)	162, 200, 501, 1023
MluCI	AATT	6 cut(s)	148, 878, 899, 1274, 1531, 1630
MluI	ACGCGT	1 cut(s)	872
MlyI	GAGTC	2 cut(s)	48, 1145
MmeI	TCCRAC	2 cut(s)	1194, 1213
MroI	TCCGGA	1 cut(s)	501
MroXI	GAANNNNTTC	2 cut(s)	285, 806
MslI	CAYNNNNRTG	1 cut(s)	414
MspA1I	CMGCKG	4 cut(s)	229, 242, 514, 866
MspCI	CTTAAG	1 cut(s)	824
MspI	CCGG	2 cut(s)	236, 502
MspR9I	CCNGG	4 cut(s)	236, 333, 1484, 1587
Mva1269I	GAATGC	2 cut(s)	806, 960
MvaI	CCWGG	3 cut(s)	333, 1484, 1587
MvnI	CGCG	1 cut(s)	874
MwoI	GCNNNNNNNGC	6 cut(s)	65, 116, 131, 184, 453, 923
NciI	CCSGG	1 cut(s)	236
NcoI	CCATGG	1 cut(s)	129
NdeI	CATATG	3 cut(s)	154, 608, 1498
NdeII	GATC	4 cut(s)	602, 1141, 1270, 1325
NheI	GCTAGC	1 cut(s)	443
NlaIII	CATG	9 cut(s)	80, 133, 178, 433, 589, 619, 634, 1151, 1222
NlaIV	GGNNCC	5 cut(s)	111, 604, 1166, 1427, 1428
NmuCI	GTSAC	3 cut(s)	553, 1115, 1262
NspI	RCATGY	3 cut(s)	178, 619, 1222
NspV	TTCGAA	1 cut(s)	19
OliI	CACNNNNGTG	1 cut(s)	414
PaeI	GCATGC	1 cut(s)	619
PagI	TCATGA	1 cut(s)	76
PceI	AGGCCT	1 cut(s)	35
PciI	ACATGT	1 cut(s)	1218
PciSI	GCTCTTC	1 cut(s)	276
PctI	GAATGC	2 cut(s)	806, 960
PdmI	GAANNNNTTC	2 cut(s)	285, 806
PfeI	GAWTC	6 cut(s)	16, 73, 911, 1341, 1670, 1697
PkrI	GCNGC	9 cut(s)	51, 136, 139, 231, 241, 305, 513, 868, 1317
PleI	GAGTC	2 cut(s)	48, 1145
PpsI	GAGTC	2 cut(s)	48, 1145
Ppu21I	YACGTR	1 cut(s)	1598
PpuMI	RGGWCCY	1 cut(s)	1426
PscI	ACATGT	1 cut(s)	1218
PshAI	GACNNNNGTC	1 cut(s)	1485
PshBI	ATTAAT	2 cut(s)	42, 471
Psp124BI	GAGCTC	2 cut(s)	200, 501
Psp1406I	AACGTT	1 cut(s)	697
Psp5II	RGGWCCY	1 cut(s)	1426
Psp6I	CCWGG	3 cut(s)	331, 1482, 1585
PspEI	GGTNACC	1 cut(s)	536
PspFI	CCCAGC	2 cut(s)	862, 1203
PspGI	CCWGG	3 cut(s)	331, 1482, 1585
PspN4I	GGNNCC	5 cut(s)	111, 604, 1166, 1427, 1428
PspPI	GGNCC	2 cut(s)	791, 1426
PspPPI	RGGWCCY	1 cut(s)	1426
PstI	CTGCAG	1 cut(s)	1320
PsuI	RGATCY	2 cut(s)	602, 1325
PvuII	CAGCTG	3 cut(s)	229, 514, 866
RsaI	GTAC	3 cut(s)	532, 1166, 1596
RsaNI	GTAC	3 cut(s)	531, 1165, 1595
RseI	CAYNNNNRTG	1 cut(s)	414
SacI	GAGCTC	2 cut(s)	200, 501
SapI	GCTCTTC	1 cut(s)	276
SatI	GCNGC	9 cut(s)	50, 135, 138, 230, 240, 304, 512, 867, 1316
Sau3AI	GATC	4 cut(s)	602, 1141, 1270, 1325
Sau96I	GGNCC	2 cut(s)	791, 1426
SchI	GAGTC	2 cut(s)	48, 1145
ScrFI	CCNGG	4 cut(s)	236, 333, 1484, 1587
SduI	GDGCHC	4 cut(s)	162, 200, 501, 1023
SexAI	ACCWGGT	1 cut(s)	1585
SfaNI	GCATC	3 cut(s)	102, 1302, 1430
SfcI	CTRYAG	1 cut(s)	1316
SfuI	TTCGAA	1 cut(s)	19
SinI	GGWCC	1 cut(s)	1426
SmiMI	CAYNNNNRTG	1 cut(s)	414
SmlI	CTYRAG	4 cut(s)	193, 683, 824, 1258
SmoI	CTYRAG	4 cut(s)	193, 683, 824, 1258
SpeI	ACTAGT	1 cut(s)	5
SphI	GCATGC	1 cut(s)	619
Sse9I	AATT	6 cut(s)	148, 878, 899, 1274, 1531, 1630
SseBI	AGGCCT	1 cut(s)	35
SsiI	CCGC	2 cut(s)	242, 937
SspMI	CTAG	5 cut(s)	6, 444, 641, 741, 963
SstI	GAGCTC	2 cut(s)	200, 501
StuI	AGGCCT	1 cut(s)	35
StyD4I	CCNGG	4 cut(s)	234, 331, 1482, 1585
StyI	CCWWGG	1 cut(s)	129
TaaI	ACNGT	4 cut(s)	86, 832, 1212, 1289
TaiI	ACGT	3 cut(s)	296, 700, 1600
TaqI	TCGA	3 cut(s)	19, 1438, 1492
TasI	AATT	6 cut(s)	148, 878, 899, 1274, 1531, 1630
TatI	WGTACW	1 cut(s)	530
TfiI	GAWTC	6 cut(s)	16, 73, 911, 1341, 1670, 1697
TscAI	CASTG	3 cut(s)	91, 763, 1473
TseFI	GTSAC	3 cut(s)	553, 1115, 1262
TseI	GCWGC	9 cut(s)	49, 134, 137, 229, 239, 303, 511, 866, 1315
Tsp45I	GTSAC	3 cut(s)	553, 1115, 1262
TspDTI	ATGAA	8 cut(s)	141, 446, 457, 602, 970, 1050, 1385, 1653
TspGWI	ACGGA	2 cut(s)	465, 1634
TspRI	CASTG	3 cut(s)	91, 763, 1473
Vha464I	CTTAAG	1 cut(s)	824
VpaK11BI	GGWCC	1 cut(s)	1426
VspI	ATTAAT	2 cut(s)	42, 471
XapI	RAATTY	1 cut(s)	878
XceI	RCATGY	3 cut(s)	178, 619, 1222
XcmI	CCANNNNNNNNNTGG	2 cut(s)	339, 416
XmiI	GTMKAC	1 cut(s)	676
XmnI	GAANNNNTTC	2 cut(s)	285, 806
XspI	CTAG	5 cut(s)	6, 444, 641, 741, 963

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.