Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002708

Physical Location & Seq

Genomic Coordinates Forward (+)

13381123 .. 13386253

Gene Structure 5131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G012780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGCCGCCCGAGGCGGAAGAAGCGGAGGAGGGGGCGCAGTACTCGTTTGCTATGGAGTACAAAGGTCCTCCGGTGAGTTACGATCTTCCGAAGGCGGTTCCGATCAACGTCGACAGAATCCCCGTCGCGGCTGTCGTCGGACAGGTGTCTATTCCGGATAAGTTGTCGTTGCCGGTGGTCCAGCCGGTTCTCGCGCCCGGTTCGCTGATGAAAACGTTCTCCAAAGAGCTCAAGGCTTCCCAGTCGACGGTGTCTCCGACCTCGGTGATCGCTTTCGATCGGAGGAGCGAGGACGACACGACCAAGGAGTTGGAAGGGTCGGAATCGGCGACGGTCTCGCCGGCGTCGGTGATTGGGTTCGAGGAGAGAGCGGAGGAGAGGGGGGCTGGCTGTGGTGCTTTGTCCGGAGAATTGAGTAGCTCCGGCGGGTTGGAGTTTTCGAACGGGTTGAATTACGGGTCGGGTGAGCTGTCCGATGTGAATAATGGTAGCTGGGCTTGGAGCGGAGGAGCATTGACGGCTCCGAGTGGTGGGAAGGAGAGTTTGGATTTGAATCAACCCGAACCCGATTGGGCTTCCTCCGAGTCCGTTTTGAGCTTGGACTATCCGTCTTCTCGGGTTTCCTCCACCAAGGCTGTGGATTGCGATGTGAAACGACCCGCTGTAGTGACGTTTCGGGACATTGAATCTGATGATGATGACGATGGTGTTGAGGAAGATGAGGCTGAGGTTGTGCCGGTGAAGCGGGAGCCTCAGAGCAAAGGAAAGAAGAAGTCTTGTTATAGATGTCTCAAGGGGACTAGGTTCACTGAGAAGGAGGTGTGCATTGTTTGTGACGCCAAGTATTGTAGTAGTTGTGTTCTGAGAGCTATGGGGTCTATGCCCGAAGGCCGAAAATGTGTTACTTGTATTGGATTTCCTATTGATGAGAACAAAAGAGGATGCTTGGGGAAGTGCTCTAGATGCTCAAGCGGCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0004857 GO:0005095 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009898 GO:0009987 GO:0016020 GO:0016247 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0030234 GO:0030695 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0034260 GO:0035639 GO:0036094 GO:0043086 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0048519 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051346 GO:0051716 GO:0060589 GO:0065007 GO:0065009 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0098772 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-alpha

Protein Analysis

326

Amino Acids

34.63

Weight (kDa)

4.66

Isoelectric Point (pI)

54.79

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23460
fragaria_vesca	FvH4_1g14590 FvH4_1g14590
malus_domestica	MD00G1067300.v1.1 MD02G1159500.v1.1
prunus_persica	Prupe.7G144400_v2.0.a1
pyrus_communis	pycom02g12580 pycom15g23720
rosa_chinensis	RchiOBHm_Chr1g0367271 RchiOBHm_Chr1g0367281 RchiOBHm_Chr1g0367331 RchiOBHm_Chr2g0103371
rosa_laevigata	RLG00000017231 RLG00000027279 RLG00000027284
rosa_multiflora	Rmu_sc0001555.1_g000003 Rmu_sc0002434.1_g000010 Rmu_sc0011000.1_g000013
rosa_roxburghii	Rroxscaffold_2G00139680 Rroxscaffold_4G00289390 Rroxscaffold_4G00289470 Rroxscaffold_4G00289480
rosa_rugosa	Rorug02G0114700 Rorug06G0002300
rosa_samantha	Rh1AG342600 Rh1BG303200 Rh1BG303600 Rh1BG303700 Rh1CG319500 Rh1CG319700 Rh1DG335600 Rh1DG336000 Rh1DG336100 Rh2AG162700 Rh2BG169800 Rh2CG169900 Rh2DG168800
rosa_wichuraiana	Rw1G030440 Rw2G012780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	371, 504
AccI	GTMKAC	2 cut(s)	111, 245
AccII	CGCG	2 cut(s)	128, 194
AccIII	TCCGGA	2 cut(s)	154, 404
AclI	AACGTT	1 cut(s)	215
AcyI	GRCGYC	2 cut(s)	344, 837
AfaI	GTAC	2 cut(s)	41, 59
AfiI	CCNNNNNNNGG	3 cut(s)	127, 247, 530
AgsI	TTSAA	3 cut(s)	451, 553, 686
AhdI	GACNNNNNGTC	1 cut(s)	607
AjuI	GAANNNNNNNTTGG	2 cut(s)	527, 559
AluBI	AGCT	6 cut(s)	229, 420, 469, 492, 597, 869
AluI	AGCT	6 cut(s)	229, 420, 469, 492, 597, 869
Alw21I	GWGCWC	2 cut(s)	231, 959
Alw26I	GTCTC	3 cut(s)	258, 340, 794
Ama87I	CYCGRG	2 cut(s)	8, 615
Aor13HI	TCCGGA	2 cut(s)	154, 404
AoxI	GGCC	1 cut(s)	889
ApeKI	GCWGC	1 cut(s)	975
Asp700I	GAANNNNTTC	1 cut(s)	215
AspLEI	GCGC	2 cut(s)	37, 196
AspS9I	GGNCC	2 cut(s)	65, 178
AsuC2I	CCSGG	1 cut(s)	198
AsuHPI	GGTGA	5 cut(s)	85, 277, 361, 476, 751
AsuII	TTCGAA	1 cut(s)	440
AvaI	CYCGRG	2 cut(s)	8, 615
AvaII	GGWCC	2 cut(s)	65, 178
BanII	GRGCYC	1 cut(s)	231
BbsI	GAAGAC	1 cut(s)	603
Bbv12I	GWGCWC	2 cut(s)	231, 959
BbvCI	CCTCAGC	1 cut(s)	726
BbvI	GCAGC	1 cut(s)	962
BccI	CCATC	1 cut(s)	698
BceAI	ACGGC	1 cut(s)	534
BcnI	CCSGG	1 cut(s)	198
BcoDI	GTCTC	3 cut(s)	258, 340, 794
BfaI	CTAG	2 cut(s)	801, 960
BfmI	CTRYAG	1 cut(s)	663
BisI	GCNGC	4 cut(s)	5, 129, 973, 976
BlsI	GCNGC	4 cut(s)	6, 130, 974, 977
BmcAI	AGTACT	1 cut(s)	41
Bme1390I	CCNGG	1 cut(s)	198
Bme18I	GGWCC	2 cut(s)	65, 178
BmeRI	GACNNNNNGTC	1 cut(s)	607
BmeT110I	CYCGRG	2 cut(s)	8, 615
BmgT120I	GGNCC	2 cut(s)	65, 178
BmiI	GGNNCC	3 cut(s)	99, 522, 750
BmrFI	CCNGG	1 cut(s)	198
BmrI	ACTGGG	1 cut(s)	235
BmsI	GCATC	2 cut(s)	932, 953
BmuI	ACTGGG	1 cut(s)	235
BoxI	GACNNNNGTC	1 cut(s)	145
BpiI	GAAGAC	1 cut(s)	603
Bpu10I	CCTNAGC	1 cut(s)	726
Bpu14I	TTCGAA	1 cut(s)	440
BpuEI	CTTGAG	3 cut(s)	215, 776, 952
BpuMI	CCSGG	1 cut(s)	198
BsaBI	GATNNNNATC	1 cut(s)	552
BsaHI	GRCGYC	2 cut(s)	344, 837
BsaI	GGTCTC	1 cut(s)	340
BsaJI	CCNNGG	4 cut(s)	9, 261, 303, 630
BsaWI	WCCGGW	3 cut(s)	70, 154, 404
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	3 cut(s)	127, 247, 530
Bse118I	RCCGGY	4 cut(s)	172, 184, 340, 736
Bse1I	ACTGG	1 cut(s)	241
Bse8I	GATNNNNATC	1 cut(s)	552
BseAI	TCCGGA	2 cut(s)	154, 404
BseDI	CCNNGG	4 cut(s)	9, 261, 303, 630
BseGI	GGATG	1 cut(s)	947
BseJI	GATNNNNATC	1 cut(s)	552
BseLI	CCNNNNNNNGG	3 cut(s)	127, 247, 530
BseMII	CTCAG	4 cut(s)	717, 767, 801, 854
BseNI	ACTGG	1 cut(s)	241
BseRI	GAGGAG	5 cut(s)	41, 298, 377, 389, 522
BseXI	GCAGC	1 cut(s)	962
BseYI	CCCAGC	1 cut(s)	492
Bsh1236I	CGCG	2 cut(s)	128, 194
Bsh1285I	CGRYCG	1 cut(s)	280
BshFI	GGCC	1 cut(s)	891
BsiEI	CGRYCG	1 cut(s)	280
BsiHKAI	GWGCWC	2 cut(s)	231, 959
BsiHKCI	CYCGRG	2 cut(s)	8, 615
BsiSI	CCGG	9 cut(s)	71, 155, 173, 185, 198, 341, 405, 423, 737
BslFI	GGGAC	2 cut(s)	692, 811
BslI	CCNNNNNNNGG	3 cut(s)	127, 247, 530
BsmAI	GTCTC	3 cut(s)	258, 340, 794
BsmFI	GGGAC	2 cut(s)	692, 811
BsnI	GGCC	1 cut(s)	891
Bso31I	GGTCTC	1 cut(s)	340
BsoBI	CYCGRG	2 cut(s)	8, 615
Bsp119I	TTCGAA	1 cut(s)	440
Bsp1286I	GDGCHC	2 cut(s)	231, 959
Bsp13I	TCCGGA	2 cut(s)	154, 404
Bsp143I	GATC	4 cut(s)	82, 102, 267, 277
BspANI	GGCC	1 cut(s)	891
BspCNI	CTCAG	4 cut(s)	718, 766, 802, 855
BspEI	TCCGGA	2 cut(s)	154, 404
BspFNI	CGCG	2 cut(s)	128, 194
BspLI	GGNNCC	3 cut(s)	99, 522, 750
BspT104I	TTCGAA	1 cut(s)	440
BspTNI	GGTCTC	1 cut(s)	340
BsrBI	CCGCTC	2 cut(s)	371, 504
BsrFI	RCCGGY	4 cut(s)	172, 184, 340, 736
BsrI	ACTGG	1 cut(s)	241
BssAI	RCCGGY	4 cut(s)	172, 184, 340, 736
BssECI	CCNNGG	4 cut(s)	9, 261, 303, 630
BssMI	GATC	4 cut(s)	82, 102, 267, 277
BssNI	GRCGYC	2 cut(s)	344, 837
BssT1I	CCWWGG	2 cut(s)	303, 630
Bst4CI	ACNGT	2 cut(s)	250, 334
BstACI	GRCGYC	2 cut(s)	344, 837
BstBI	TTCGAA	1 cut(s)	440
BstC8I	GCNNGC	2 cut(s)	342, 388
BstDEI	CTNAG	4 cut(s)	726, 753, 810, 863
BstF5I	GGATG	1 cut(s)	947
BstFNI	CGCG	2 cut(s)	128, 194
BstHHI	GCGC	2 cut(s)	37, 196
BstKTI	GATC	4 cut(s)	85, 105, 270, 280
BstMAI	GTCTC	3 cut(s)	258, 340, 794
BstMBI	GATC	4 cut(s)	82, 102, 267, 277
BstMCI	CGRYCG	1 cut(s)	280
BstMWI	GCNNNNNNNGC	6 cut(s)	20, 202, 518, 742, 963, 972
BstPAI	GACNNNNGTC	1 cut(s)	145
BstSCI	CCNGG	1 cut(s)	196
BstSFI	CTRYAG	1 cut(s)	663
BstUI	CGCG	2 cut(s)	128, 194
BstV1I	GCAGC	1 cut(s)	962
BstV2I	GAAGAC	1 cut(s)	603
BstXI	CCANNNNNNTGG	2 cut(s)	310, 637
BsuRI	GGCC	1 cut(s)	891
BtgZI	GCGATG	1 cut(s)	660
BtsCI	GGATG	1 cut(s)	947
BtsIMutI	CAGTG	1 cut(s)	807
Cac8I	GCNNGC	2 cut(s)	342, 388
CfoI	GCGC	2 cut(s)	37, 196
Cfr10I	RCCGGY	4 cut(s)	172, 184, 340, 736
Cfr13I	GGNCC	2 cut(s)	65, 178
CseI	GACGC	2 cut(s)	333, 845
Csp6I	GTAC	2 cut(s)	40, 58
CviQI	GTAC	2 cut(s)	40, 58
DdeI	CTNAG	4 cut(s)	726, 753, 810, 863
DpnI	GATC	4 cut(s)	84, 104, 269, 279
DpnII	GATC	4 cut(s)	82, 102, 267, 277
DriI	GACNNNNNGTC	1 cut(s)	607
Eam1105I	GACNNNNNGTC	1 cut(s)	607
EciI	GGCGGA	1 cut(s)	29
Ecl136II	GAGCTC	1 cut(s)	229
Eco130I	CCWWGG	2 cut(s)	303, 630
Eco24I	GRGCYC	1 cut(s)	231
Eco31I	GGTCTC	1 cut(s)	340
Eco47I	GGWCC	2 cut(s)	65, 178
Eco53kI	GAGCTC	1 cut(s)	229
Eco88I	CYCGRG	2 cut(s)	8, 615
EcoICRI	GAGCTC	1 cut(s)	229
EcoO109I	RGGNCCY	1 cut(s)	65
EcoT14I	CCWWGG	2 cut(s)	303, 630
EcoT38I	GRGCYC	1 cut(s)	231
ErhI	CCWWGG	2 cut(s)	303, 630
FaiI	YATR	4 cut(s)	53, 783, 872, 881
FaqI	GGGAC	2 cut(s)	692, 811
FauI	CCCGC	3 cut(s)	419, 667, 738
FblI	GTMKAC	2 cut(s)	111, 245
Fnu4HI	GCNGC	4 cut(s)	5, 129, 973, 976
FokI	GGATG	1 cut(s)	954
FriOI	GRGCYC	1 cut(s)	231
Fsp4HI	GCNGC	4 cut(s)	5, 129, 973, 976
FspBI	CTAG	2 cut(s)	801, 960
GlaI	GCGC	2 cut(s)	36, 195
GluI	GCNGC	4 cut(s)	5, 129, 973, 976
GsaI	CCCAGC	1 cut(s)	496
HaeIII	GGCC	1 cut(s)	891
HapII	CCGG	9 cut(s)	71, 155, 173, 185, 198, 341, 405, 423, 737
HgaI	GACGC	2 cut(s)	333, 845
HhaI	GCGC	2 cut(s)	37, 196
Hin1I	GRCGYC	2 cut(s)	344, 837
Hin6I	GCGC	2 cut(s)	35, 194
HinP1I	GCGC	2 cut(s)	35, 194
HincII	GTYRAC	2 cut(s)	112, 246
HindII	GTYRAC	2 cut(s)	112, 246
HinfI	GANTC	5 cut(s)	117, 323, 553, 584, 686
HpaII	CCGG	9 cut(s)	71, 155, 173, 185, 198, 341, 405, 423, 737
HphI	GGTGA	5 cut(s)	85, 277, 361, 476, 751
Hpy166II	GTNNAC	3 cut(s)	112, 246, 807
Hpy188III	TCNNGA	4 cut(s)	155, 405, 677, 960
Hpy8I	GTNNAC	3 cut(s)	112, 246, 807
Hpy99I	CGWCG	6 cut(s)	113, 128, 140, 250, 334, 349
HpyAV	CCTTC	5 cut(s)	85, 308, 529, 808, 881
HpyCH4III	ACNGT	2 cut(s)	250, 334
HpyCH4IV	ACGT	3 cut(s)	108, 215, 671
HpyCH4V	TGCA	1 cut(s)	825
HpyF10VI	GCNNNNNNNGC	6 cut(s)	20, 202, 518, 742, 963, 972
HpyF3I	CTNAG	4 cut(s)	726, 753, 810, 863
HpySE526I	ACGT	3 cut(s)	108, 215, 671
Hsp92I	GRCGYC	2 cut(s)	344, 837
HspAI	GCGC	2 cut(s)	35, 194
Kpn2I	TCCGGA	2 cut(s)	154, 404
KroI	GCCGGC	1 cut(s)	340
KroNI	GCCGGC	1 cut(s)	342
Kzo9I	GATC	4 cut(s)	82, 102, 267, 277
LmnI	GCTCC	6 cut(s)	285, 425, 501, 509, 526, 748
Lsp1109I	GCAGC	1 cut(s)	962
LweI	GCATC	2 cut(s)	932, 953
MaeI	CTAG	2 cut(s)	801, 960
MaeII	ACGT	3 cut(s)	108, 215, 671
MaeIII	GTNAC	4 cut(s)	77, 667, 833, 901
MalI	GATC	4 cut(s)	84, 104, 269, 279
MbiI	CCGCTC	2 cut(s)	371, 504
MboI	GATC	4 cut(s)	82, 102, 267, 277
MboII	GAAGA	5 cut(s)	29, 77, 603, 728, 781
MhlI	GDGCHC	2 cut(s)	231, 959
MluCI	AATT	2 cut(s)	410, 451
MlyI	GAGTC	1 cut(s)	593
MmeI	TCCRAC	5 cut(s)	118, 281, 291, 300, 411
MreI	CGCCGGCG	1 cut(s)	340
MroI	TCCGGA	2 cut(s)	154, 404
MroNI	GCCGGC	1 cut(s)	340
MroXI	GAANNNNTTC	1 cut(s)	215
MspA1I	CMGCKG	1 cut(s)	662
MspI	CCGG	9 cut(s)	71, 155, 173, 185, 198, 341, 405, 423, 737
MspR9I	CCNGG	1 cut(s)	198
MvnI	CGCG	2 cut(s)	128, 194
MwoI	GCNNNNNNNGC	6 cut(s)	20, 202, 518, 742, 963, 972
NaeI	GCCGGC	1 cut(s)	342
NciI	CCSGG	1 cut(s)	198
NdeII	GATC	4 cut(s)	82, 102, 267, 277
NgoMIV	GCCGGC	1 cut(s)	340
NlaIV	GGNNCC	3 cut(s)	99, 522, 750
NmuCI	GTSAC	2 cut(s)	667, 833
NspV	TTCGAA	1 cut(s)	440
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 254, 326
PdiI	GCCGGC	1 cut(s)	342
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	4 cut(s)	117, 323, 553, 686
PflFI	GACNNNGTC	1 cut(s)	250
PkrI	GCNGC	4 cut(s)	6, 130, 974, 977
Ple19I	CGATCG	1 cut(s)	280
PleI	GAGTC	1 cut(s)	592
PpsI	GAGTC	1 cut(s)	592
PpuMI	RGGWCCY	1 cut(s)	65
PshAI	GACNNNNGTC	1 cut(s)	145
Psp124BI	GAGCTC	1 cut(s)	231
Psp1406I	AACGTT	1 cut(s)	215
Psp5II	RGGWCCY	1 cut(s)	65
PspFI	CCCAGC	1 cut(s)	492
PspN4I	GGNNCC	3 cut(s)	99, 522, 750
PspPI	GGNCC	2 cut(s)	65, 178
PspPPI	RGGWCCY	1 cut(s)	65
PsyI	GACNNNGTC	1 cut(s)	250
PvuI	CGATCG	1 cut(s)	280
RsaI	GTAC	2 cut(s)	41, 59
RsaNI	GTAC	2 cut(s)	40, 58
SacI	GAGCTC	1 cut(s)	231
SalI	GTCGAC	2 cut(s)	110, 244
SatI	GCNGC	4 cut(s)	5, 129, 973, 976
Sau3AI	GATC	4 cut(s)	82, 102, 267, 277
Sau96I	GGNCC	2 cut(s)	65, 178
ScaI	AGTACT	1 cut(s)	41
SchI	GAGTC	1 cut(s)	593
ScrFI	CCNGG	1 cut(s)	198
SduI	GDGCHC	2 cut(s)	231, 959
SfaNI	GCATC	2 cut(s)	932, 953
SfcI	CTRYAG	1 cut(s)	663
SfuI	TTCGAA	1 cut(s)	440
SgrAI	CRCCGGYG	1 cut(s)	340
SinI	GGWCC	2 cut(s)	65, 178
SmlI	CTYRAG	3 cut(s)	230, 791, 967
SmoI	CTYRAG	3 cut(s)	230, 791, 967
Sse9I	AATT	2 cut(s)	410, 451
SspMI	CTAG	2 cut(s)	801, 960
SstI	GAGCTC	1 cut(s)	231
StyD4I	CCNGG	1 cut(s)	196
StyI	CCWWGG	2 cut(s)	303, 630
TaaI	ACNGT	2 cut(s)	250, 334
TaiI	ACGT	3 cut(s)	111, 218, 674
TaqI	TCGA	5 cut(s)	111, 245, 276, 360, 440
TasI	AATT	2 cut(s)	410, 451
TatI	WGTACW	2 cut(s)	39, 57
TauI	GCSGC	3 cut(s)	7, 131, 975
TfiI	GAWTC	4 cut(s)	117, 323, 553, 686
TscAI	CASTG	1 cut(s)	814
TseFI	GTSAC	2 cut(s)	667, 833
TseI	GCWGC	1 cut(s)	975
Tsp45I	GTSAC	2 cut(s)	667, 833
TspDTI	ATGAA	1 cut(s)	224
TspGWI	ACGGA	2 cut(s)	577, 597
TspRI	CASTG	1 cut(s)	814
Tth111I	GACNNNGTC	1 cut(s)	250
VpaK11BI	GGWCC	2 cut(s)	65, 178
XbaI	TCTAGA	1 cut(s)	959
XcmI	CCANNNNNNNNNTGG	1 cut(s)	634
XmiI	GTMKAC	2 cut(s)	111, 245
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	2 cut(s)	801, 960
ZrmI	AGTACT	1 cut(s)	41

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.