MD00G1135700.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001890
Physical Location & Seq
Reverse (-)
29527919 .. 29532943
5025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1135700.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGGGGTAAGGCTAGCCGACATTCACCTCTCCCAGACCCTGCTTAAAGCGGGAGCCTTGTGCACTGGGTTGACGAAGATCCTATCTTTGCTAGAGATGGCATTGAAGGCAATTTTCGTTGTCCTGTTGTCCCGTGTCGTACCCAGTGCACAAGGCTCCCGCTTTACGCAGGGTCTGGGAGAGGCTATTCTTGGTGGTACGGTTGAGGTACCAACTTTATCTGGGAAGATAGAAGTAAAAATACCAAAGGGGGTTCAGCCTGGACAACACCTGGTACTCAGAGGCAAAGGACTGCCAAAACATGGTTTTCTAGTTAAGCATGGAGATCAATATGTGCGATTCCGAGTTAAATTTCCCACTGAAATCAACGAACAGCAAAATGCTATATTAGAAGACTTTGTGGAGGAGGAAATTATTCATGGAGACGGTACTTCCAATTCCAACAAAAGAGATTGGTTTCAGAGAATCCTTGAACGTGTTTCGGAGCCTAGGTTTATGCTTCAACTCTCGATATTCGTATTGATCCTTTTGTTGCTGAACAAATCCATGGGCTCAATGCTGGCCCCAGGCAATTTTGATCGAGATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

197

Amino Acids

21.66

Weight (kDa)

9.33

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 60 - 119 5.7e-17 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 208
AccB7I CCANNNNNTGG 1 cut(s) 302
AciI CCGC 2 cut(s) 50, 160
AclWI GGATC 2 cut(s) 73, 517
AcsI RAATTY 1 cut(s) 350
AfaI GTAC 5 cut(s) 141, 199, 210, 276, 430
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 3 cut(s) 106, 473, 503
AjnI CCWGG 3 cut(s) 259, 270, 565
Alw21I GWGCWC 2 cut(s) 65, 151
Alw26I GTCTC 1 cut(s) 417
Alw44I GTGCAC 2 cut(s) 61, 147
AlwI GGATC 2 cut(s) 73, 517
AlwNI CAGNNNCTG 2 cut(s) 40, 175
AoxI GGCC 1 cut(s) 561
ApaLI GTGCAC 2 cut(s) 61, 147
ApoI RAATTY 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 414
Asp718I GGTACC 1 cut(s) 208
AspA2I CCTAGG 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 1 cut(s) 17
AsuNHI GCTAGC 1 cut(s) 13
AvrII CCTAGG 1 cut(s) 488
BaeGI GKGCMC 2 cut(s) 65, 151
BanI GGYRCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 554
BarI GAAGNNNNNNTAC 2 cut(s) 225, 257
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 2 cut(s) 65, 151
BccI CCATC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 261, 272, 567
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 4 cut(s) 14, 92, 311, 489
BlnI CCTAGG 1 cut(s) 488
Bme1390I CCNGG 3 cut(s) 261, 272, 567
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 5 cut(s) 55, 157, 210, 486, 564
BmrFI CCNGG 3 cut(s) 261, 272, 567
BmrI ACTGGG 2 cut(s) 75, 138
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 2 cut(s) 75, 138
BpiI GAAGAC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 488, 546, 565
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 70, 144
BseBI CCWGG 3 cut(s) 261, 272, 567
BseDI CCNNGG 3 cut(s) 488, 546, 565
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 292
BseNI ACTGG 2 cut(s) 70, 144
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 2 cut(s) 65, 151
BshFI GGCC 1 cut(s) 563
BshNI GGYRCC 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 65, 151
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 417
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 1 cut(s) 563
Bsp1286I GDGCHC 3 cut(s) 65, 151, 554
Bsp143I GATC 4 cut(s) 78, 325, 522, 577
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 2 cut(s) 50, 160
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 1 cut(s) 291
BspLI GGNNCC 5 cut(s) 55, 157, 210, 486, 564
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 73, 517
BspT107I GGYRCC 1 cut(s) 208
BsrI ACTGG 2 cut(s) 70, 144
BssECI CCNNGG 3 cut(s) 488, 546, 565
BssMI GATC 4 cut(s) 78, 325, 522, 577
BssT1I CCWWGG 2 cut(s) 488, 546
Bst2UI CCWGG 3 cut(s) 261, 272, 567
Bst4CI ACNGT 2 cut(s) 202, 428
BstC8I GCNNGC 2 cut(s) 15, 561
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 546
BstKTI GATC 4 cut(s) 81, 328, 525, 580
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 4 cut(s) 78, 325, 522, 577
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 3 cut(s) 261, 272, 567
BstSCI CCNGG 3 cut(s) 259, 270, 565
BstSLI GKGCMC 2 cut(s) 65, 151
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 546
BtsIMutI CAGTG 3 cut(s) 63, 151, 357
Cac8I GCNNGC 2 cut(s) 15, 561
CaiI CAGNNNCTG 2 cut(s) 40, 175
Cfr13I GGNCC 1 cut(s) 562
CsiI ACCWGGT 1 cut(s) 270
Csp6I GTAC 5 cut(s) 140, 198, 209, 275, 429
CviAII CATG 4 cut(s) 302, 320, 419, 547
CviJI RGCY 9 cut(s) 13, 17, 56, 156, 185, 259, 487, 552, 563
CviKI_1 RGCY 9 cut(s) 13, 17, 56, 156, 185, 259, 487, 552, 563
CviQI GTAC 5 cut(s) 140, 198, 209, 275, 429
DdeI CTNAG 1 cut(s) 278
DpnI GATC 4 cut(s) 80, 327, 524, 579
DpnII GATC 4 cut(s) 78, 325, 522, 577
Eco130I CCWWGG 2 cut(s) 488, 546
Eco24I GRGCYC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 259, 270, 565
EcoT14I CCWWGG 2 cut(s) 488, 546
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 2 cut(s) 488, 546
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 4 cut(s) 305, 323, 422, 550
FaiI YATR 8 cut(s) 303, 321, 333, 386, 420, 497, 548, 589
FaqI GGGAC 1 cut(s) 115
FatI CATG 4 cut(s) 301, 319, 418, 546
FauI CCCGC 2 cut(s) 43, 167
FriOI GRGCYC 1 cut(s) 554
FspBI CTAG 4 cut(s) 14, 92, 311, 489
HaeIII GGCC 1 cut(s) 563
Hin1II CATG 4 cut(s) 305, 323, 422, 550
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 2 cut(s) 339, 465
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 63, 72, 149
Hpy188I TCNGA 4 cut(s) 281, 344, 462, 484
Hpy188III TCNNGA 2 cut(s) 508, 581
Hpy8I GTNNAC 3 cut(s) 63, 72, 149
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 2 cut(s) 202, 428
HpyCH4IV ACGT 1 cut(s) 475
HpyCH4V TGCA 2 cut(s) 63, 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 278
HpySE526I ACGT 1 cut(s) 475
Hsp92II CATG 4 cut(s) 305, 323, 422, 550
KpnI GGTACC 1 cut(s) 212
Kzo9I GATC 4 cut(s) 78, 325, 522, 577
LmnI GCTCC 3 cut(s) 53, 161, 484
MabI ACCWGGT 1 cut(s) 270
MaeI CTAG 4 cut(s) 14, 92, 311, 489
MaeII ACGT 1 cut(s) 475
MalI GATC 4 cut(s) 80, 327, 524, 579
MboI GATC 4 cut(s) 78, 325, 522, 577
MboII GAAGA 3 cut(s) 88, 238, 404
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 3 cut(s) 65, 151, 554
MluCI AATT 5 cut(s) 111, 350, 411, 436, 571
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 6 cut(s) 38, 175, 199, 275, 397, 400
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 3 cut(s) 45, 315, 348
MspR9I CCNGG 3 cut(s) 261, 272, 567
MvaI CCWGG 3 cut(s) 261, 272, 567
MwoI GCNNNNNNNGC 1 cut(s) 107
NcoI CCATGG 1 cut(s) 546
NdeII GATC 4 cut(s) 78, 325, 522, 577
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 4 cut(s) 305, 323, 422, 550
NlaIV GGNNCC 5 cut(s) 55, 157, 210, 486, 564
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 339, 465
PflMI CCANNNNNTGG 1 cut(s) 302
Psp6I CCWGG 3 cut(s) 259, 270, 565
PspGI CCWGG 3 cut(s) 259, 270, 565
PspN4I GGNNCC 5 cut(s) 55, 157, 210, 486, 564
PspPI GGNCC 1 cut(s) 562
PstNI CAGNNNCTG 2 cut(s) 40, 175
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 5 cut(s) 141, 199, 210, 276, 430
RsaNI GTAC 5 cut(s) 140, 198, 209, 275, 429
SaqAI TTAA 3 cut(s) 45, 315, 348
Sau3AI GATC 4 cut(s) 78, 325, 522, 577
Sau96I GGNCC 1 cut(s) 562
ScrFI CCNGG 3 cut(s) 261, 272, 567
SduI GDGCHC 3 cut(s) 65, 151, 554
SetI ASST 5 cut(s) 30, 210, 273, 478, 494
SexAI ACCWGGT 1 cut(s) 270
Sse9I AATT 5 cut(s) 111, 350, 411, 436, 571
SsiI CCGC 2 cut(s) 50, 160
SspMI CTAG 4 cut(s) 14, 92, 311, 489
StyD4I CCNGG 3 cut(s) 259, 270, 565
StyI CCWWGG 2 cut(s) 488, 546
TaaI ACNGT 2 cut(s) 202, 428
TaiI ACGT 1 cut(s) 478
TaqI TCGA 2 cut(s) 509, 580
TasI AATT 5 cut(s) 111, 350, 411, 436, 571
TfiI GAWTC 2 cut(s) 339, 465
Tru1I TTAA 3 cut(s) 45, 315, 348
Tru9I TTAA 3 cut(s) 45, 315, 348
TscAI CASTG 3 cut(s) 70, 151, 364
TspDTI ATGAA 1 cut(s) 407
TspRI CASTG 3 cut(s) 70, 151, 364
Van91I CCANNNNNTGG 1 cut(s) 302
VneI GTGCAC 2 cut(s) 61, 147
XapI RAATTY 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 488
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 4 cut(s) 14, 92, 311, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.