pycom08g16440

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001890
Physical Location & Seq
Reverse (-)
16419779 .. 16425208
5430 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16440.1

Sequence Viewer

Length: 750 bp
ATGCTATGGGCGTGGCTATCCACTAATGATGATTATGGAAATATTTCTTCTAAAAGGAGTCAGTTTGAGTTACAGTTCCTCCATTCACATCAACATGCAGTACGTGTAAAGCTCAATGATCGATCGGGCCAAATAATCAAGGAATATTGCTTGTCATGCAGAGGATTAGGGATTATTGAAGGTGTTAGACAGGTTGAAGTTTCGATACCAGAAGGTGTGGATTCTGGAGATACAATCAGTGTTCCAGAGGCTGGGAATAGTGGGGCACGAGGAAGCGCGGCAGGCTGGCTATACATTAAACTAAAGGTTGACGAAGATCCTATCTTTGCTAGAGACGGTGCAGATCTTTATGTGGACTCGAATATTAGCTTTACACAAGCTATTCTTGGTGGTACGGTTGAGGTACCAGCTTTATCTGGGAAGATTGAAGTAAAAATACCAAAGCGGGTTCAGCCTGGACAACGTCTGGTACTCAGAGGCAAACGACTGCCAAAACATGGTTTTCTAGTTAAGCATGGAGATCAATATGTGCAATTCCGGGTTAAATTTCCCACTGAACTCAACGAACGGCAAAATGCAATATTAGAAGACTTCGTGGATGGGGAAATTATTCATGGAGACGGTACTTCCAATTCCAACAAAAGAGATTGGTTTCAGAGAATCCTTGAACGTATTTCGGAGCCTAGGTTAATGCTTCAACTCTCGATATTCGTATTGATCCTTTTGTTGCTGAACAAATCCATGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

250

Amino Acids

27.91

Weight (kDa)

7.8

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 41 - 184 1.7e-35 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccB7I CCANNNNNTGG 2 cut(s) 251, 497
AccII CGCG 1 cut(s) 278
AciI CCGC 2 cut(s) 278, 445
AclWI GGATC 2 cut(s) 311, 712
AcsI RAATTY 1 cut(s) 545
AfaI GTAC 5 cut(s) 102, 394, 405, 471, 625
AfiI CCNNNNNNNGG 2 cut(s) 251, 497
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 5 cut(s) 179, 197, 428, 668, 698
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 4 cut(s) 112, 369, 380, 410
AluI AGCT 4 cut(s) 112, 369, 380, 410
Alw26I GTCTC 2 cut(s) 327, 612
AlwI GGATC 2 cut(s) 311, 712
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 545
Asp700I GAANNNNTTC 2 cut(s) 43, 609
Asp718I GGTACC 1 cut(s) 403
AspA2I CCTAGG 1 cut(s) 683
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 539
AvrII CCTAGG 1 cut(s) 683
BaeGI GKGCMC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 403
BarI GAAGNNNNNNTAC 4 cut(s) 189, 221, 420, 452
BauI CACGAG 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 594
BccI CCATC 1 cut(s) 593
BceAI ACGGC 1 cut(s) 584
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 2 cut(s) 327, 612
BfaI CTAG 3 cut(s) 330, 506, 684
BglII AGATCT 1 cut(s) 343
BisI GCNGC 1 cut(s) 279
BlnI CCTAGG 1 cut(s) 683
BlsI GCNGC 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 456, 539
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 405, 681
BmrFI CCNGG 2 cut(s) 456, 539
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 539
Bsa29I ATCGAT 1 cut(s) 121
BsaAI YACGTR 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 683, 741
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 671, 701
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 497
BseBI CCWGG 1 cut(s) 456
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 2 cut(s) 683, 741
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 2 cut(s) 251, 497
BseMII CTCAG 1 cut(s) 487
BseSI GKGCMC 1 cut(s) 268
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 538
BslI CCNNNNNNNGG 2 cut(s) 251, 497
BsmAI GTCTC 2 cut(s) 327, 612
BsmBI CGTCTC 2 cut(s) 327, 612
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 6 cut(s) 118, 122, 316, 343, 520, 717
Bsp19I CCATGG 1 cut(s) 741
BspACI CCGC 2 cut(s) 278, 445
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 486
BspDI ATCGAT 1 cut(s) 121
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 405, 681
BspPI GGATC 2 cut(s) 311, 712
BspT107I GGYRCC 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 683, 741
BssMI GATC 6 cut(s) 118, 122, 316, 343, 520, 717
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 683, 741
Bst2BI CACGAG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 4 cut(s) 75, 338, 397, 623
BstBAI YACGTR 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 283, 287
BstDEI CTNAG 1 cut(s) 473
BstDSI CCRYGG 1 cut(s) 741
BstF5I GGATG 1 cut(s) 604
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 6 cut(s) 121, 125, 319, 346, 523, 720
BstMAI GTCTC 2 cut(s) 327, 612
BstMBI GATC 6 cut(s) 118, 122, 316, 343, 520, 717
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 3 cut(s) 156, 282, 451
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 454, 537
BstSLI GKGCMC 1 cut(s) 268
BstUI CGCG 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 2 cut(s) 316, 343
BstYI RGATCY 2 cut(s) 316, 343
Bsu15I ATCGAT 1 cut(s) 121
BsuRI GGCC 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 121
BtgI CCRYGG 1 cut(s) 741
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 2 cut(s) 244, 552
Cac8I GCNNGC 2 cut(s) 283, 287
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 127
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 5 cut(s) 101, 393, 404, 470, 624
CviAII CATG 6 cut(s) 95, 156, 497, 515, 614, 742
CviQI GTAC 5 cut(s) 101, 393, 404, 470, 624
DdeI CTNAG 1 cut(s) 473
DpnI GATC 6 cut(s) 120, 124, 318, 345, 522, 719
DpnII GATC 6 cut(s) 118, 122, 316, 343, 520, 717
Eco130I CCWWGG 2 cut(s) 683, 741
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 683, 741
ErhI CCWWGG 2 cut(s) 683, 741
Esp3I CGTCTC 2 cut(s) 327, 612
FaeI CATG 6 cut(s) 98, 159, 500, 518, 617, 745
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 6 cut(s) 94, 155, 496, 514, 613, 741
FauI CCCGC 1 cut(s) 438
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 3 cut(s) 330, 506, 684
GlaI GCGC 1 cut(s) 277
GluI GCNGC 1 cut(s) 279
GsaI CCCAGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 538
HhaI GCGC 1 cut(s) 278
Hin1II CATG 6 cut(s) 98, 159, 500, 518, 617, 745
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 58, 221, 356, 660
HpaII CCGG 1 cut(s) 538
Hpy166II GTNNAC 2 cut(s) 310, 355
Hpy188I TCNGA 3 cut(s) 476, 657, 679
Hpy188III TCNNGA 3 cut(s) 225, 245, 703
Hpy8I GTNNAC 2 cut(s) 310, 355
HpyAV CCTTC 2 cut(s) 173, 206
HpyCH4III ACNGT 4 cut(s) 75, 338, 397, 623
HpyCH4IV ACGT 3 cut(s) 103, 463, 670
HpyCH4V TGCA 5 cut(s) 98, 159, 341, 532, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 282, 451
HpyF3I CTNAG 1 cut(s) 473
HpySE526I ACGT 3 cut(s) 103, 463, 670
Hsp92II CATG 6 cut(s) 98, 159, 500, 518, 617, 745
HspAI GCGC 1 cut(s) 276
KpnI GGTACC 1 cut(s) 407
Kzo9I GATC 6 cut(s) 118, 122, 316, 343, 520, 717
LmnI GCTCC 1 cut(s) 679
MaeI CTAG 3 cut(s) 330, 506, 684
MaeII ACGT 3 cut(s) 103, 463, 670
MaeIII GTNAC 1 cut(s) 69
MalI GATC 6 cut(s) 120, 124, 318, 345, 522, 719
MboI GATC 6 cut(s) 118, 122, 316, 343, 520, 717
MboII GAAGA 4 cut(s) 39, 326, 433, 599
MflI RGATCY 2 cut(s) 316, 343
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 4 cut(s) 533, 545, 606, 631
MlyI GAGTC 2 cut(s) 67, 350
MmeI TCCRAC 1 cut(s) 660
MnlI CCTC 6 cut(s) 89, 155, 241, 263, 394, 470
MroXI GAANNNNTTC 2 cut(s) 43, 609
MseI TTAA 4 cut(s) 297, 510, 543, 689
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 2 cut(s) 456, 539
MvaI CCWGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 3 cut(s) 156, 282, 451
NciI CCSGG 1 cut(s) 539
NcoI CCATGG 1 cut(s) 741
NdeII GATC 6 cut(s) 118, 122, 316, 343, 520, 717
NlaIII CATG 6 cut(s) 98, 159, 500, 518, 617, 745
NlaIV GGNNCC 2 cut(s) 405, 681
NspI RCATGY 1 cut(s) 98
PdmI GAANNNNTTC 2 cut(s) 43, 609
PfeI GAWTC 2 cut(s) 221, 660
PflFI GACNNNGTC 1 cut(s) 462
PflMI CCANNNNNTGG 2 cut(s) 251, 497
PkrI GCNGC 1 cut(s) 280
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 2 cut(s) 66, 350
PpsI GAGTC 2 cut(s) 66, 350
Ppu21I YACGTR 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 405, 681
PspPI GGNCC 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 2 cut(s) 316, 343
PsyI GACNNNGTC 1 cut(s) 462
PvuI CGATCG 1 cut(s) 125
RsaI GTAC 5 cut(s) 102, 394, 405, 471, 625
RsaNI GTAC 5 cut(s) 101, 393, 404, 470, 624
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 4 cut(s) 297, 510, 543, 689
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 6 cut(s) 118, 122, 316, 343, 520, 717
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 2 cut(s) 67, 350
ScrFI CCNGG 2 cut(s) 456, 539
SduI GDGCHC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 4 cut(s) 533, 545, 606, 631
SsiI CCGC 2 cut(s) 278, 445
SspI AATATT 4 cut(s) 43, 146, 364, 582
SspMI CTAG 3 cut(s) 330, 506, 684
StyD4I CCNGG 2 cut(s) 454, 537
StyI CCWWGG 2 cut(s) 683, 741
TaaI ACNGT 4 cut(s) 75, 338, 397, 623
TaiI ACGT 3 cut(s) 106, 466, 673
TaqI TCGA 4 cut(s) 121, 203, 359, 704
TasI AATT 4 cut(s) 533, 545, 606, 631
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 221, 660
Tru1I TTAA 4 cut(s) 297, 510, 543, 689
Tru9I TTAA 4 cut(s) 297, 510, 543, 689
TscAI CASTG 2 cut(s) 244, 559
TspDTI ATGAA 1 cut(s) 602
TspRI CASTG 2 cut(s) 244, 559
Tth111I GACNNNGTC 1 cut(s) 462
Van91I CCANNNNNTGG 2 cut(s) 251, 497
XapI RAATTY 1 cut(s) 545
XceI RCATGY 1 cut(s) 98
XmaJI CCTAGG 1 cut(s) 683
XmnI GAANNNNTTC 2 cut(s) 43, 609
XspI CTAG 3 cut(s) 330, 506, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.