Rorug07G0308000

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001890
Physical Location & Seq
Forward (+)
30951132 .. 30958878
7747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0308000.1

Sequence Viewer

Length: 3702 bp
ATGTCAGTAGCAGCAATGGGGGTGTCTGATCCAAGCCAACTCCACTTGAAGAAAGAGCTCACCCAGATTCGAAAGGCCGCCCGCGTTCTCCGCGATCCGGGGACCACTTCTTCTTGGCGCTCGCCTTTGGCTTCCTCTAGATCTGCGGCGGGGGTTCCTTCTACTTGTTCCACTTCCCACAATACCAACAATGGCGGCGGCGGCGGCGGCGACAACAAGAAGGTGTTCCTGTACAACTGGAAGGGCTCTTCCAAATCGTCGAGAAATGATGACTACGAAGAGGAGGAGGAGGAGGAGGAGGATGATTATGGTGTTGAAGATGAGGATGAAGCTTCTTCTGTTGCGGCATTGAGTGTGGATGACAGCTTGAGCCACGGAGATTCCAGGAGTGACGCCGGTGGTGGTGGTCAGAGCCGGTCGTTGATGATGCTCCGGCGCAGATATCCGAATCTTCTACCGCCGCTCAAAGATGGTAATGGTAATGGTAAGAAAGCAAGTAAGAAAATCGAGTCCCATTCTCATGTTTTGTCCAAACATCAGCAGAAAGAGTTGATTCTTGGTAGGAACTTAAGAAAGGGAGGCCGGGATGAGCAATCCGATGACACTGAGGATTACTCTAATTCTGAGGATCTGAGGCACATTAGAGGGGCTTCCCCATTGCTCTTGAAGCTTAGGCACAAGCATTGGCCTCCGGCCTCCACTAATGTTAGGAGAAATGATAGTAGAGCAGAGGACTCTTCTTATTGCTATAGCACTCCTGCATTGTCGACTAGTTCTTATAATAGGTATGGTGTTCGGAATCCAAGTACTGTTGGATCTTGGGATGGAACTGCGACTTCAATGAATGATGGGGATGATGAGGTTGAGGACCATTTGGATATTCCGGGTAGCAAAGGATGTGGGATTCCTTGTTATTGGTCCAAAAAGACCCCAAAGCACAGAGGCATGTATGGAAGCTGTTGCTCCCCTTCGCTTTCTGATACTATAAGGAGAAAAGGAAGTGTCCTTTTGTGTGGAAGTCAAAATGTTTATCCTAGACGACGGGGGTCGTCGTCAGGTTCCAATAAGCGGAGAATTGCATCTAGAAGTGCGCAAGGTGTTCTGCCATTGCTAAACAATAGTGGTGATGGTAGAGGAGGGTCATCCATAGGAACTGGGAGGAGTGATGACGAACTCTCCACAAACTTTGGGGAACTTGATTTGGAAGCTTTGAGTAGATTGGATGGACGGAGATGGTCAAGCTGCCGAAGTCAAGAAGGCTTGGAGATTGTAGCTCTGAGTGGAGTGGGGGAAGAGGAAAGCACTCCAGATAGTGTTAGAAGTTTTAGCCAGAAGTACAAGCCGATGTTCTTTCGTGAATTGATTGGCCAGAATATTGTGGTGCAATCACTTATAAATGCTATTGATAGGTGTAGGATTGCTCCCGTTTATCTTTTCCAGGGACCCCGTGGAACTGGAAAAACGTCAACAGCTAGAATTTTTGCTGCTTCCTTGAATTGCCTAGCTCCTGATGAAACTAAACCCTGTGGCTACTGCAGGGAGTGCACTGATTTTATGTCTGGTAAGAATAAGGATCTATTGGAAGTTGATGGTACCAATAAGAAAGGAATTGATAAAGTCAGGTACCTATTGAAAACTCTATCTGTGGCTCCCTCATCAGCATCATCAAGGTATAAGGTTTTTGTAATTGATGAGTGTCACTTATTGCCTTCTAAGACATGGTTGGCATTTCTTAAATATTTGGAAGAACCACCGCAGCGAGTTGTCTTCATATTCATTACAACTGATTTGGATAATGTTCCTCGTACCATACAATCTCGGTGTCAGAAATATCTCTTCAACAAAATTAAGGATAGTGAGATTGTGGCCAGGTTGAGGAAAATTGCTGTTGAAGAGAACCTAGATGTTGGACCAGATGCATTAGATTTGATTGCTTTGAATGCAGATGGTTCACTTCGAGATGCAGAAACTATGTTGGACCAGTTGAGTTTGCTGGGGAAAAGAATTTCAACATCTCTTGTGAATGAACTTGTGGGAGTTGTTTCCGATGACAAGTTACTAGATCTTTTGGAGTTAGCAATGTCATCGGACACTGCAGAAACAGTGAAAAGGGCCAGAGATTTGATGGACTCCGGGGTTGATCCCATGGTTTTGATGTCTCAGTTGGCCAGCTTAATTATGGACATCATTGCGGGAACCAACAGTACTAATGATGTCAAACATGACTATGGCGCTCGGAACTTGACTGAAGCAGAATTGGAGAGATTAAAACATGCTCTGAAGATTCTTTCGGAGGCTGAAAAACAGCTTAGGGTTTCAAGTGAGCGCTCAACATGGTTTACAGCGACTCTGTTGCAGCTTGGTTCAGTGCCTTCTCCAGACCTTAGTCACTCATGCAGCAGGAGGCATAGCTGCAAGACAACTGAGGATGATTCTTCAAGTGCTTCAACTTCAAGAGAAGCAGCCACTTACAAACAGAAGTTAGATGGTCACTATATACTACAAAAATCAACTCAACAAGCTTCTGTACAAAGAGCACCAAATGACAATTCCAACCATCAGGGAGATTTGTTGTCAAGAAATTATGGTTTCGGTGTGAATACCAAGCCCTCACATGATCAGTTCGTGGATAGTGGAGCCTCAATTCCCTTGTGTGGAAATGTTATGGCTGGAAATATGAGCCTTAGATGTGTAAACTCTGAAAAGTTAAATGATGTATGGGCACAGTGTATTGAGAAGTGTCATTCAAAGACATTGAGGCAGCTACTACATTCTCATGGAAAGCTAGTGTCTATATCTGAAGCTGAAGGTGTTCTGGTTGCTTATGTTGCATTTGAAGATGGAAGTATCAAATCTAGAGCAGAGAGGTTTGTGAGCAGTATCACAAATTCAATGGAAATAGTTCTCAGACGCAATGTAGAGGTTAGAATTGTCCACTTGCCAGGTGGGGAGGCTTCTCTAAATGGTCCAAGTCCGGTTCACTTGCTCGAAGGTGTAAAGCAGGCAGGAACTATAGCAGAACTTGTCAGGGAAAGGAAAAGAGTTGGTTCAAATGCCACAGATGGTTATTCCAATTGTTCTCTGTTTCTGGATGGGACCCACAAGTCAACCTCTGATTCATCAGACTTAGTTGCAGATGGCAATGCTCAAACAAGTGATACAAGAGAGAGCGGACAGGAGATCCCAATGCAGAGAATAGAATCCATTATTCGCGACCAAAGGTTAGAGACTGCGTGGCTACAGGTTGTAGAGAAAGGCACCCCTGGTTCTTTGAGTCGTTTGAAACCTGAAAAGAATCAGGTCCTGCCTCAGGATGGCATTTATTACGAAGATCAGATGGAATCGATGAATTCAATGGGATTATCCTCTCAGCAATGGGATGATGGATTAAACCATGAAGTAAAAATCTTGAGTGCTAACAGTGGGAAGGTCATTCAGCAGGACCAAATAGGTAAAAGGGTTGATCATTATCCTATGTCCCCGAGTTTACTACATGAAAAGGGAAAGTCCGACAAAGATCACCTGGGCGATGAATCAGGGTCAGGTAGAGGAGGTTGCAGTGGATTTTTTCATTGTTATAACACTAGACGTCGTAAAAGAGGAAAGGTAAAAGGGACCCCTGTTCAACCACGCAAAGGTAGACGGTTTTCATTGTTTGGTGTGTGTGGCAAGTCAAGGAAGAAAGCAGAGAAGACAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

1233

Amino Acids

135.4

Weight (kDa)

8.3

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 457 - 616 2.7e-34 DNA polymerase III, delta subunit
AAA PF00004 478 - 613 8.6e-09 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 620 - 661 2.3e-08 DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3 PF12169 670 - 786 1.9e-08 DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI PF23007 896 - 977 6.3e-31 STICHEL, DnaA_N-like alpha-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 780, 1394, 3579
AasI GACNNNNNNGTC 1 cut(s) 3103
AatII GACGTC 1 cut(s) 3592
Acc16I TGCGCA 1 cut(s) 1092
Acc65I GGTACC 2 cut(s) 1592, 1623
AccB1I GGYRCC 3 cut(s) 1592, 1623, 3257
AccBSI CCGCTC 2 cut(s) 463, 3171
AccI GTMKAC 2 cut(s) 767, 3640
AccII CGCG 3 cut(s) 84, 93, 3213
AclWI GGATC 7 cut(s) 23, 89, 636, 823, 1581, 2135, 3175
AcoI YGGCCR 3 cut(s) 1366, 1866, 2166
AcsI RAATTY 4 cut(s) 1476, 2004, 2887, 3349
AcuI CTGAAG 4 cut(s) 2268, 2300, 2820, 2826
AcyI GRCGYC 2 cut(s) 393, 3589
AfaI GTAC 8 cut(s) 233, 808, 1337, 1594, 1625, 1807, 2206, 2529
AfeI AGCGCT 1 cut(s) 2327
AfiI CCNNNNNNNGG 7 cut(s) 97, 1068, 1875, 2654, 3310, 3314, 3635
AflII CTTAAG 1 cut(s) 568
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 6 cut(s) 383, 1437, 1868, 2941, 3262, 3522
AjuI GAANNNNNNNTTGG 2 cut(s) 2533, 2565
Alw21I GWGCWC 3 cut(s) 60, 1547, 2539
Alw26I GTCTC 2 cut(s) 2163, 3221
Alw44I GTGCAC 1 cut(s) 1543
AlwI GGATC 7 cut(s) 23, 89, 636, 823, 1581, 2135, 3175
AlwNI CAGNNNCTG 1 cut(s) 3304
Ama87I CYCGRG 1 cut(s) 3481
Aor51HI AGCGCT 1 cut(s) 2327
AoxI GGCC 8 cut(s) 75, 580, 686, 693, 1366, 1866, 2112, 2166
ApaLI GTGCAC 1 cut(s) 1543
ApeKI GCWGC 9 cut(s) 11, 1242, 1484, 1756, 2356, 2397, 2412, 2462, 2761
ApoI RAATTY 4 cut(s) 1476, 2004, 2887, 3349
Asp700I GAANNNNTTC 3 cut(s) 224, 2811, 2901
Asp718I GGTACC 2 cut(s) 1592, 1623
AspLEI GCGC 5 cut(s) 120, 438, 1093, 2234, 2328
AsuC2I CCSGG 4 cut(s) 99, 584, 885, 2134
AsuHPI GGTGA 3 cut(s) 52, 1136, 3512
AsuII TTCGAA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 3481
AxyI CCTNAGG 1 cut(s) 3309
BaeGI GKGCMC 2 cut(s) 1547, 2725
BalI TGGCCA 3 cut(s) 1368, 1868, 2168
BanI GGYRCC 3 cut(s) 1592, 1623, 3257
BanII GRGCYC 2 cut(s) 60, 248
BbsI GAAGAC 2 cut(s) 1759, 3698
Bbv12I GWGCWC 3 cut(s) 60, 1547, 2539
BbvI GCAGC 9 cut(s) 23, 1229, 1471, 1768, 2368, 2399, 2409, 2474, 2773
BcgI CGANNNNNNTGC 4 cut(s) 2067, 2101, 2335, 2369
BciT130I CCWGG 6 cut(s) 385, 1439, 1870, 2943, 3264, 3524
BclI TGATCA 2 cut(s) 2617, 3463
BcnI CCSGG 4 cut(s) 99, 584, 885, 2134
BcoDI GTCTC 2 cut(s) 2163, 3221
BcuI ACTAGT 1 cut(s) 770
BfmI CTRYAG 5 cut(s) 748, 1534, 2094, 3012, 3239
BfoI RGCGCY 3 cut(s) 121, 2235, 2329
BfrI CTTAAG 1 cut(s) 568
BglII AGATCT 2 cut(s) 140, 2062
BmcAI AGTACT 2 cut(s) 808, 2206
BmeT110I CYCGRG 1 cut(s) 3481
BmrI ACTGGG 1 cut(s) 1164
BmsI GCATC 5 cut(s) 417, 1088, 1670, 1906, 1951
BmuI ACTGGG 1 cut(s) 1164
BoxI GACNNNNGTC 2 cut(s) 1045, 2385
BpiI GAAGAC 2 cut(s) 1759, 3698
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 2 cut(s) 1290, 2361
Bpu10I CCTNAGC 2 cut(s) 671, 2309
Bpu14I TTCGAA 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 388, 3430
BpuMI CCSGG 4 cut(s) 99, 584, 885, 2134
Bsa29I ATCGAT 1 cut(s) 3344
BsaBI GATNNNNATC 1 cut(s) 3468
BsaHI GRCGYC 2 cut(s) 393, 3589
BsaJI CCNNGG 8 cut(s) 98, 373, 1438, 1447, 2133, 2145, 3262, 3523
BsaWI WCCGGW 1 cut(s) 2974
BsaXI ACNNNNNCTCC 2 cut(s) 1160, 1190
Bsc4I CCNNNNNNNGG 7 cut(s) 97, 1068, 1875, 2654, 3310, 3314, 3635
Bse118I RCCGGY 2 cut(s) 395, 414
Bse1I ACTGG 4 cut(s) 242, 1159, 1459, 1981
Bse21I CCTNAGG 1 cut(s) 3309
Bse3DI GCAATG 8 cut(s) 21, 656, 1106, 2085, 2187, 2920, 3148, 3380
Bse8I GATNNNNATC 1 cut(s) 3468
BseBI CCWGG 6 cut(s) 385, 1439, 1870, 2943, 3264, 3524
BseCI ATCGAT 1 cut(s) 3344
BseDI CCNNGG 8 cut(s) 98, 373, 1438, 1447, 2133, 2145, 3262, 3523
BseJI GATNNNNATC 1 cut(s) 3468
BseLI CCNNNNNNNGG 7 cut(s) 97, 1068, 1875, 2654, 3310, 3314, 3635
BseMI GCAATG 8 cut(s) 21, 656, 1106, 2085, 2187, 2920, 3148, 3380
BseMII CTCAG 9 cut(s) 597, 615, 623, 1268, 2174, 2415, 2920, 3323, 3383
BseNI ACTGG 4 cut(s) 242, 1159, 1459, 1981
BseRI GAGGAG 9 cut(s) 296, 299, 302, 305, 308, 311, 1149, 1174, 3564
BseSI GKGCMC 2 cut(s) 1547, 2725
BseXI GCAGC 9 cut(s) 23, 1229, 1471, 1768, 2368, 2399, 2409, 2474, 2773
BseYI CCCAGC 1 cut(s) 1993
Bsh1236I CGCG 3 cut(s) 84, 93, 3213
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 8 cut(s) 77, 582, 688, 695, 1368, 1868, 2114, 2168
BshNI GGYRCC 3 cut(s) 1592, 1623, 3257
BshVI ATCGAT 1 cut(s) 3344
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 3 cut(s) 60, 1547, 2539
BsiHKCI CYCGRG 1 cut(s) 3481
BsiSI CCGG 9 cut(s) 98, 396, 415, 433, 583, 692, 884, 2133, 2975
BslFI GGGAC 6 cut(s) 115, 496, 1455, 3109, 3463, 3628
BslI CCNNNNNNNGG 7 cut(s) 97, 1068, 1875, 2654, 3310, 3314, 3635
BsmAI GTCTC 2 cut(s) 2163, 3221
BsmFI GGGAC 6 cut(s) 115, 496, 1455, 3109, 3463, 3628
BsmI GAATGC 1 cut(s) 1945
BsnI GGCC 8 cut(s) 77, 582, 688, 695, 1368, 1868, 2114, 2168
BsoBI CYCGRG 1 cut(s) 3481
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 5 cut(s) 60, 248, 1547, 2539, 2725
Bsp1407I TGTACA 2 cut(s) 231, 2527
Bsp19I CCATGG 1 cut(s) 2145
Bsp68I TCGCGA 1 cut(s) 3213
BspANI GGCC 8 cut(s) 77, 582, 688, 695, 1368, 1868, 2114, 2168
BspCNI CTCAG 9 cut(s) 598, 616, 624, 1269, 2173, 2416, 2919, 3322, 3382
BspDI ATCGAT 1 cut(s) 3344
BspFNI CGCG 3 cut(s) 84, 93, 3213
BspMAI CTGCAG 2 cut(s) 1538, 2098
BspPI GGATC 7 cut(s) 23, 89, 636, 823, 1581, 2135, 3175
BspQI GCTCTTC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 70
BspT107I GGYRCC 3 cut(s) 1592, 1623, 3257
BspTI CTTAAG 1 cut(s) 568
BsrBI CCGCTC 2 cut(s) 463, 3171
BsrDI GCAATG 8 cut(s) 21, 656, 1106, 2085, 2187, 2920, 3148, 3380
BsrFI RCCGGY 2 cut(s) 395, 414
BsrGI TGTACA 2 cut(s) 231, 2527
BsrI ACTGG 4 cut(s) 242, 1159, 1459, 1981
BssAI RCCGGY 2 cut(s) 395, 414
BssECI CCNNGG 8 cut(s) 98, 373, 1438, 1447, 2133, 2145, 3262, 3523
BssNI GRCGYC 2 cut(s) 393, 3589
BssT1I CCWWGG 1 cut(s) 2145
Bst2UI CCWGG 6 cut(s) 385, 1439, 1870, 2943, 3264, 3524
Bst4CI ACNGT 6 cut(s) 811, 2104, 2204, 2727, 3422, 3645
Bst6I CTCTTC 6 cut(s) 253, 273, 742, 1287, 1841, 1887
BstACI GRCGYC 2 cut(s) 393, 3589
BstAFI CTTAAG 1 cut(s) 568
BstAPI GCANNNNNTGC 1 cut(s) 1542
BstAUI TGTACA 2 cut(s) 231, 2527
BstBI TTCGAA 1 cut(s) 70
BstC8I GCNNGC 4 cut(s) 82, 122, 2170, 3003
BstDSI CCRYGG 3 cut(s) 373, 1447, 2145
BstENI CCTNNNNNAGG 1 cut(s) 3308
BstFNI CGCG 3 cut(s) 84, 93, 3213
BstH2I RGCGCY 3 cut(s) 121, 2235, 2329
BstHHI GCGC 5 cut(s) 120, 438, 1093, 2234, 2328
BstMAI GTCTC 2 cut(s) 2163, 3221
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 8 cut(s) 90, 201, 204, 207, 667, 1542, 1940, 2828
BstNI CCWGG 6 cut(s) 385, 1439, 1870, 2943, 3264, 3524
BstNSI RCATGY 2 cut(s) 949, 2276
BstPAI GACNNNNGTC 2 cut(s) 1045, 2385
BstSFI CTRYAG 5 cut(s) 748, 1534, 2094, 3012, 3239
BstSLI GKGCMC 2 cut(s) 1547, 2725
BstUI CGCG 3 cut(s) 84, 93, 3213
BstV1I GCAGC 9 cut(s) 23, 1229, 1471, 1768, 2368, 2399, 2409, 2474, 2773
BstV2I GAAGAC 2 cut(s) 1759, 3698
BstX2I RGATCY 6 cut(s) 140, 628, 815, 1573, 2062, 3180
BstYI RGATCY 6 cut(s) 140, 628, 815, 1573, 2062, 3180
Bsu15I ATCGAT 1 cut(s) 3344
Bsu36I CCTNAGG 1 cut(s) 3309
BsuRI GGCC 8 cut(s) 77, 582, 688, 695, 1368, 1868, 2114, 2168
BsuTUI ATCGAT 1 cut(s) 3344
BtgI CCRYGG 3 cut(s) 373, 1447, 2145
BtgZI GCGATG 1 cut(s) 3543
BtsI GCAGTG 2 cut(s) 2091, 3565
BtsIMutI CAGTG 8 cut(s) 603, 1545, 2091, 2109, 2373, 2732, 3427, 3565
BtuMI TCGCGA 1 cut(s) 3213
Cac8I GCNNGC 4 cut(s) 82, 122, 2170, 3003
CaiI CAGNNNCTG 1 cut(s) 3304
CfoI GCGC 5 cut(s) 120, 438, 1093, 2234, 2328
Cfr10I RCCGGY 2 cut(s) 395, 414
ClaI ATCGAT 1 cut(s) 3344
CseI GACGC 2 cut(s) 401, 2919
Csp6I GTAC 8 cut(s) 232, 807, 1336, 1593, 1624, 1806, 2205, 2528
CspCI CAANNNNNGTGG 4 cut(s) 880, 915, 1168, 1203
CviQI GTAC 8 cut(s) 232, 807, 1336, 1593, 1624, 1806, 2205, 2528
DrdI GACNNNNNNGTC 1 cut(s) 3103
DseDI GACNNNNNNGTC 1 cut(s) 3103
EaeI YGGCCR 3 cut(s) 1366, 1866, 2166
Eam1104I CTCTTC 6 cut(s) 253, 273, 742, 1287, 1841, 1887
EarI CTCTTC 6 cut(s) 253, 273, 742, 1287, 1841, 1887
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 2145
Eco24I GRGCYC 2 cut(s) 60, 248
Eco32I GATATC 1 cut(s) 443
Eco47III AGCGCT 1 cut(s) 2327
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 4 cut(s) 2268, 2300, 2820, 2826
Eco81I CCTNAGG 1 cut(s) 3309
Eco88I CYCGRG 1 cut(s) 3481
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 3308
EcoO109I RGGNCCY 4 cut(s) 1442, 3096, 3301, 3615
EcoRI GAATTC 1 cut(s) 3349
EcoRII CCWGG 6 cut(s) 383, 1437, 1868, 2941, 3262, 3522
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 2145
EcoT22I ATGCAT 1 cut(s) 1921
EcoT38I GRGCYC 2 cut(s) 60, 248
ErhI CCWWGG 1 cut(s) 2145
FaqI GGGAC 6 cut(s) 115, 496, 1455, 3109, 3463, 3628
FauI CCCGC 3 cut(s) 89, 142, 2185
FbaI TGATCA 2 cut(s) 2617, 3463
FblI GTMKAC 2 cut(s) 767, 3640
FriOI GRGCYC 2 cut(s) 60, 248
FspI TGCGCA 1 cut(s) 1092
GlaI GCGC 5 cut(s) 119, 437, 1092, 2233, 2327
GsaI CCCAGC 1 cut(s) 1997
GsuI CTGGAG 2 cut(s) 1290, 2361
HaeII RGCGCY 3 cut(s) 121, 2235, 2329
HaeIII GGCC 8 cut(s) 77, 582, 688, 695, 1368, 1868, 2114, 2168
HapII CCGG 9 cut(s) 98, 396, 415, 433, 583, 692, 884, 2133, 2975
HgaI GACGC 2 cut(s) 401, 2919
HhaI GCGC 5 cut(s) 120, 438, 1093, 2234, 2328
Hin1I GRCGYC 2 cut(s) 393, 3589
Hin6I GCGC 5 cut(s) 118, 436, 1091, 2232, 2326
HinP1I GCGC 5 cut(s) 118, 436, 1091, 2232, 2326
HincII GTYRAC 3 cut(s) 768, 1467, 3108
HindII GTYRAC 3 cut(s) 768, 1467, 3108
HindIII AAGCTT 4 cut(s) 330, 668, 1206, 2520
HpaII CCGG 9 cut(s) 98, 396, 415, 433, 583, 692, 884, 2133, 2975
HphI GGTGA 3 cut(s) 52, 1136, 3512
Hpy99I CGWCG 4 cut(s) 262, 1044, 1054, 3594
HpyCH4III ACNGT 6 cut(s) 811, 2104, 2204, 2727, 3422, 3645
HpyCH4IV ACGT 2 cut(s) 1463, 3589
HpyF10VI GCNNNNNNNGC 8 cut(s) 90, 201, 204, 207, 667, 1542, 1940, 2828
HpySE526I ACGT 2 cut(s) 1463, 3589
Hsp92I GRCGYC 2 cut(s) 393, 3589
HspAI GCGC 5 cut(s) 118, 436, 1091, 2232, 2326
KflI GGGWCCC 3 cut(s) 1442, 3096, 3615
KpnI GGTACC 2 cut(s) 1596, 1627
Ksp22I TGATCA 2 cut(s) 2617, 3463
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 6 cut(s) 435, 968, 1426, 1510, 1654, 2636
Lsp1109I GCAGC 9 cut(s) 23, 1229, 1471, 1768, 2368, 2399, 2409, 2474, 2773
LweI GCATC 5 cut(s) 417, 1088, 1670, 1906, 1951
MaeII ACGT 2 cut(s) 1463, 3589
MaeIII GTNAC 5 cut(s) 389, 1697, 2055, 2387, 2489
MbiI CCGCTC 2 cut(s) 463, 3171
MfeI CAATTG 1 cut(s) 3073
MflI RGATCY 6 cut(s) 140, 628, 815, 1573, 2062, 3180
MhlI GDGCHC 5 cut(s) 60, 248, 1547, 2539, 2725
MlsI TGGCCA 3 cut(s) 1368, 1868, 2168
MluNI TGGCCA 3 cut(s) 1368, 1868, 2168
MlyI GAGTC 5 cut(s) 518, 728, 2123, 2341, 3283
MmeI TCCRAC 5 cut(s) 793, 1888, 1956, 2577, 3534
Mox20I TGGCCA 3 cut(s) 1368, 1868, 2168
Mph1103I ATGCAT 1 cut(s) 1921
MroXI GAANNNNTTC 3 cut(s) 224, 2811, 2901
MscI TGGCCA 3 cut(s) 1368, 1868, 2168
MseI TTAA 7 cut(s) 569, 1734, 1848, 2174, 2267, 2708, 3389
MslI CAYNNNNRTG 3 cut(s) 519, 2226, 2775
Msp20I TGGCCA 3 cut(s) 1368, 1868, 2168
MspCI CTTAAG 1 cut(s) 568
MspI CCGG 9 cut(s) 98, 396, 415, 433, 583, 692, 884, 2133, 2975
MunI CAATTG 1 cut(s) 3073
Mva1269I GAATGC 1 cut(s) 1945
MvaI CCWGG 6 cut(s) 385, 1439, 1870, 2943, 3264, 3524
MvnI CGCG 3 cut(s) 84, 93, 3213
MwoI GCNNNNNNNGC 8 cut(s) 90, 201, 204, 207, 667, 1542, 1940, 2828
NciI CCSGG 4 cut(s) 99, 584, 885, 2134
NcoI CCATGG 1 cut(s) 2145
NmuCI GTSAC 4 cut(s) 389, 1697, 2387, 2489
NruI TCGCGA 1 cut(s) 3213
NsbI TGCGCA 1 cut(s) 1092
NsiI ATGCAT 1 cut(s) 1921
NspI RCATGY 2 cut(s) 949, 2276
NspV TTCGAA 1 cut(s) 70
PciSI GCTCTTC 1 cut(s) 253
PctI GAATGC 1 cut(s) 1945
PdmI GAANNNNTTC 3 cut(s) 224, 2811, 2901
PfoI TCCNGGA 1 cut(s) 383
PleI GAGTC 5 cut(s) 517, 728, 2123, 2341, 3282
PpsI GAGTC 5 cut(s) 517, 728, 2123, 2341, 3282
PpuMI RGGWCCY 4 cut(s) 1442, 3096, 3301, 3615
PshAI GACNNNNGTC 2 cut(s) 1045, 2385
PsiI TTATAA 3 cut(s) 780, 1394, 3579
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 4 cut(s) 1442, 3096, 3301, 3615
Psp6I CCWGG 6 cut(s) 383, 1437, 1868, 2941, 3262, 3522
PspFI CCCAGC 1 cut(s) 1993
PspGI CCWGG 6 cut(s) 383, 1437, 1868, 2941, 3262, 3522
PspPPI RGGWCCY 4 cut(s) 1442, 3096, 3301, 3615
PsrI GAACNNNNNNTAC 2 cut(s) 2188, 2220
PstI CTGCAG 2 cut(s) 1538, 2098
PstNI CAGNNNCTG 1 cut(s) 3304
PsuI RGATCY 6 cut(s) 140, 628, 815, 1573, 2062, 3180
RruI TCGCGA 1 cut(s) 3213
RsaI GTAC 8 cut(s) 233, 808, 1337, 1594, 1625, 1807, 2206, 2529
RsaNI GTAC 8 cut(s) 232, 807, 1336, 1593, 1624, 1806, 2205, 2528
RseI CAYNNNNRTG 3 cut(s) 519, 2226, 2775
SacI GAGCTC 1 cut(s) 60
SalI GTCGAC 1 cut(s) 766
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 7 cut(s) 569, 1734, 1848, 2174, 2267, 2708, 3389
ScaI AGTACT 2 cut(s) 808, 2206
SchI GAGTC 5 cut(s) 518, 728, 2123, 2341, 3283
SduI GDGCHC 5 cut(s) 60, 248, 1547, 2539, 2725
SfaNI GCATC 5 cut(s) 417, 1088, 1670, 1906, 1951
SfcI CTRYAG 5 cut(s) 748, 1534, 2094, 3012, 3239
SfuI TTCGAA 1 cut(s) 70
SgrAI CRCCGGYG 1 cut(s) 395
SmiMI CAYNNNNRTG 3 cut(s) 519, 2226, 2775
SmlI CTYRAG 3 cut(s) 367, 568, 3409
SmoI CTYRAG 3 cut(s) 367, 568, 3409
SpeI ACTAGT 1 cut(s) 770
SspI AATATT 2 cut(s) 1375, 1739
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 1 cut(s) 2145
TaaI ACNGT 6 cut(s) 811, 2104, 2204, 2727, 3422, 3645
TaiI ACGT 2 cut(s) 1466, 3592
TaqI TCGA 7 cut(s) 70, 260, 507, 767, 1957, 2988, 3344
TatI WGTACW 5 cut(s) 231, 806, 1335, 2204, 2527
TauI GCSGC 9 cut(s) 80, 149, 198, 201, 204, 207, 210, 347, 463
Tru1I TTAA 7 cut(s) 569, 1734, 1848, 2174, 2267, 2708, 3389
Tru9I TTAA 7 cut(s) 569, 1734, 1848, 2174, 2267, 2708, 3389
TscAI CASTG 8 cut(s) 610, 1552, 2098, 2109, 2373, 2732, 3427, 3565
TseFI GTSAC 4 cut(s) 389, 1697, 2387, 2489
TseI GCWGC 9 cut(s) 11, 1242, 1484, 1756, 2356, 2397, 2412, 2462, 2761
Tsp45I GTSAC 4 cut(s) 389, 1697, 2387, 2489
TspGWI ACGGA 2 cut(s) 390, 1243
TspRI CASTG 8 cut(s) 610, 1552, 2098, 2109, 2373, 2732, 3427, 3565
Vha464I CTTAAG 1 cut(s) 568
VneI GTGCAC 1 cut(s) 1543
XagI CCTNNNNNAGG 1 cut(s) 3308
XapI RAATTY 4 cut(s) 1476, 2004, 2887, 3349
XbaI TCTAGA 3 cut(s) 137, 1082, 2855
XceI RCATGY 2 cut(s) 949, 2276
XcmI CCANNNNNNNNNTGG 4 cut(s) 1445, 2122, 2176, 2942
XmiI GTMKAC 2 cut(s) 767, 3640
XmnI GAANNNNTTC 3 cut(s) 224, 2811, 2901
ZraI GACGTC 1 cut(s) 3590
ZrmI AGTACT 2 cut(s) 808, 2206
Zsp2I ATGCAT 1 cut(s) 1921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.