MD00G1154100.v1.1

Integrator complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004459
Physical Location & Seq
Forward (+)
33826367 .. 33829780
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1154100.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGGTTGGCATTTCAAATCTTGCACTAAGAATTATGTTGAAGCAAATGTGAAACTAGCTCTACTTTATGATTGGCTCTTTTTCGAGGAAAGAGTAGACAATATTATGGATATTGAACCTGCAATGTTATTAATGGTCTATTCTATACCCAGATACATTGATGTAACTCACACTATTCTTGAATTTTTCTTCCTTCTTATGGACAACTATGATGTGGAACATTACGATATCTTAGCTAAAGGTGTGTCATCTTCTTTTAGTGTACTGGTTAAGAGAGGAGTGATTCGTTCATTTGATGTCTTGACCTCTTGTGATGCACTGTCTCCTATATTGAAAGAGAGGCTTGGTAGGTTTTTGTCACGTAGGAAATTTGAAATCTCTAAGGAGGTTCAACAAGCTTGTCGTCCTGGTGATTTTGTGAGTTTGAACTCTTCATGTATGGAAACTCCATTACCATTAACAGAATGGCAACCTAAATGTAGCCAAGGAGACAGATTTGGAACCAAATTAGCTGAAACTAAAGTTATGATATCAGATGATGCAGCTCCTTCCCATAGTCCATTAGTAGTGGCCAGGGAAGGTCAAGTTCATGATATAGACAATTTGATTCATAATCTTGGAGAAGCAATTAAAAATTCAAATCCAATGGCTCTTCATACTTTGGAAGAACTGTTACTTTTATTTGTGAGTTTTGATGGCCAAGGACCAAAGAGTGGCTTCATTCTCCCTGATGTTTTATCTTCTATAATGAGAATGTTGTATGAATTGAGTGGGTCCAAATTATTTTCTCCTCTCATCCAGATAGTCCTGAAAACGAATGGTTTTCCAGTTGGAGCAAGGTTATTATCGTATGCATCAAGACTAGCTTATGAGGCACACATGGTGGGTTTTTTTGGAGGTGCCATGGTTGACGTTAATGCTATTACTCCAAGTGAATCAGGCATCCATCTGCTGCTATATCATGTCAATGGGTACTTTTCTTTTTTGCATGGCAGAACTGAAAACTCATATGAAACCATTGAATCTATTTCTAAAGCAGAAAAGAAGTTAGTTGCAATGTTAGTCGATGGTGCCTTTGCTGCCTACAAATGCTTCCTTATGTATTCAAGAACTGCCTTATTCAAGGATGCTGAAAATTCTCTGCCACGGCTCCTTTTTTCTGACATAATGACTTGTTCTGTGTGGGAGAGGAAGCGATTAAAATTCTTGTTTTCCAGTGTCTATTCTCATCTTTCTGATATGTCCATTGGTGAGGATGGCATCATCAAGCTACTTGAACTACTTTCAAAGTTTTTCTGGTTGGAGAAATTAGATGCAAATGCGAGTGCCATTGCGGTTGAAGGTCTTCTTACACTCTGTAGTTGCTGTCCACCCATGCCTAAGCTTGTTGGGGCTGTCATGTTATTACCTAACAGTGTCTTTCAGGATTTTGCTTCTGCTGTTATGGCTAAATGGGCTGTATCTAATGGCTTCATGCTATTTGAAAGCTTGGCTGAGTTTTCAGAAAAAGTTGAGGGCAAAAATGGGGATCTCGTCATTCATGATTCAGCAGGAATGATAAACCGCTCTGCCATTTTGTGGTTATTGAAGTATTTTAATGGGAGTGACACTGATGGGTTATGTAATGCGTTTCATTGGAAGGTCTTTCTTTTGAAAGTGGACATGCAATCTGAAGATGAAAAGGTTGTATTCAGAATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ints3

Protein Analysis

568

Amino Acids

63.77

Weight (kDa)

5.68

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ints3_N PF10189 2 - 115 1.1e-34 Integrator complex subunit 3 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 127
AccB1I GGYRCC 2 cut(s) 899, 1070
AccB7I CCANNNNNTGG 2 cut(s) 713, 1578
AccBSI CCGCTC 1 cut(s) 1566
AccI GTMKAC 1 cut(s) 96
AciI CCGC 2 cut(s) 1334, 1564
AclWI GGATC 1 cut(s) 1536
AcoI YGGCCR 2 cut(s) 570, 697
AcsI RAATTY 5 cut(s) 182, 368, 634, 1135, 1202
AcuI CTGAAG 1 cut(s) 1692
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 2 cut(s) 264, 974
AfiI CCNNNNNNNGG 3 cut(s) 199, 713, 1578
AjnI CCWGG 2 cut(s) 406, 572
AluBI AGCT 9 cut(s) 59, 236, 398, 512, 545, 866, 1270, 1384, 1488
AluI AGCT 9 cut(s) 59, 236, 398, 512, 545, 866, 1270, 1384, 1488
Alw26I GTCTC 2 cut(s) 327, 483
AlwI GGATC 1 cut(s) 1536
AoxI GGCC 2 cut(s) 570, 697
ApeKI GCWGC 3 cut(s) 542, 952, 1079
ApoI RAATTY 5 cut(s) 182, 368, 634, 1135, 1202
AseI ATTAAT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 1344
AspS9I GGNCC 2 cut(s) 704, 774
AsuHPI GGTGA 2 cut(s) 422, 1262
AvaII GGWCC 2 cut(s) 704, 774
BalI TGGCCA 2 cut(s) 572, 699
BanI GGYRCC 2 cut(s) 899, 1070
BarI GAAGNNNNNNTAC 2 cut(s) 657, 689
BbsI GAAGAC 1 cut(s) 1337
BbvI GCAGC 3 cut(s) 554, 939, 1066
BccI CCATC 5 cut(s) 689, 954, 1061, 1250, 1607
BceAI ACGGC 1 cut(s) 1163
BciT130I CCWGG 2 cut(s) 408, 574
BcoDI GTCTC 2 cut(s) 327, 483
BfaI CTAG 2 cut(s) 56, 863
BfmI CTRYAG 1 cut(s) 1357
BfuAI ACCTGC 1 cut(s) 127
BisI GCNGC 3 cut(s) 543, 953, 1080
BlsI GCNGC 3 cut(s) 544, 954, 1081
Bme1390I CCNGG 2 cut(s) 408, 574
Bme18I GGWCC 2 cut(s) 704, 774
BmgT120I GGNCC 2 cut(s) 704, 774
BmiI GGNNCC 5 cut(s) 502, 775, 901, 1072, 1151
BmrFI CCNGG 2 cut(s) 408, 574
BmsI GCATC 7 cut(s) 304, 529, 863, 951, 1117, 1269, 1303
BpiI GAAGAC 1 cut(s) 1337
Bpu10I CCTNAGC 1 cut(s) 1380
BsaAI YACGTR 1 cut(s) 362
BsaJI CCNNGG 5 cut(s) 484, 573, 700, 903, 1145
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 713, 1578
Bse1I ACTGG 3 cut(s) 270, 827, 1215
Bse3DI GCAATG 3 cut(s) 129, 1062, 1329
BseBI CCWGG 2 cut(s) 408, 574
BseDI CCNNGG 5 cut(s) 484, 573, 700, 903, 1145
BseGI GGATG 4 cut(s) 795, 942, 1132, 1261
BseLI CCNNNNNNNGG 3 cut(s) 199, 713, 1578
BseMI GCAATG 3 cut(s) 129, 1062, 1329
BseMII CTCAG 1 cut(s) 1485
BseNI ACTGG 3 cut(s) 270, 827, 1215
BseRI GAGGAG 2 cut(s) 291, 780
BseXI GCAGC 3 cut(s) 554, 939, 1066
BshFI GGCC 2 cut(s) 572, 699
BshNI GGYRCC 2 cut(s) 899, 1070
BslI CCNNNNNNNGG 3 cut(s) 199, 713, 1578
BsmAI GTCTC 2 cut(s) 327, 483
BsnI GGCC 2 cut(s) 572, 699
Bsp143I GATC 1 cut(s) 1528
Bsp19I CCATGG 1 cut(s) 903
BspACI CCGC 2 cut(s) 1334, 1564
BspANI GGCC 2 cut(s) 572, 699
BspCNI CTCAG 1 cut(s) 1486
BspHI TCATGA 2 cut(s) 589, 1540
BspLI GGNNCC 5 cut(s) 502, 775, 901, 1072, 1151
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 1 cut(s) 1536
BspQI GCTCTTC 1 cut(s) 657
BspT107I GGYRCC 2 cut(s) 899, 1070
BsrBI CCGCTC 1 cut(s) 1566
BsrDI GCAATG 3 cut(s) 129, 1062, 1329
BsrI ACTGG 3 cut(s) 270, 827, 1215
BssECI CCNNGG 5 cut(s) 484, 573, 700, 903, 1145
BssMI GATC 1 cut(s) 1528
BssT1I CCWWGG 3 cut(s) 484, 700, 903
Bst2UI CCWGG 2 cut(s) 408, 574
Bst4CI ACNGT 3 cut(s) 321, 672, 1415
Bst6I CTCTTC 2 cut(s) 436, 657
BstBAI YACGTR 1 cut(s) 362
BstDEI CTNAG 5 cut(s) 27, 232, 381, 1380, 1494
BstDSI CCRYGG 2 cut(s) 903, 1145
BstF5I GGATG 4 cut(s) 795, 942, 1132, 1261
BstKTI GATC 1 cut(s) 1531
BstMAI GTCTC 2 cut(s) 327, 483
BstMBI GATC 1 cut(s) 1528
BstMWI GCNNNNNNNGC 4 cut(s) 872, 1079, 1445, 1454
BstNI CCWGG 2 cut(s) 408, 574
BstNSI RCATGY 1 cut(s) 1666
BstSCI CCNGG 2 cut(s) 406, 572
BstSFI CTRYAG 1 cut(s) 1357
BstV1I GCAGC 3 cut(s) 554, 939, 1066
BstV2I GAAGAC 1 cut(s) 1337
BstX2I RGATCY 1 cut(s) 1528
BstYI RGATCY 1 cut(s) 1528
BsuRI GGCC 2 cut(s) 572, 699
BtgI CCRYGG 2 cut(s) 903, 1145
BtsCI GGATG 4 cut(s) 795, 942, 1132, 1261
BtsIMutI CAGTG 4 cut(s) 317, 1222, 1420, 1608
BveI ACCTGC 1 cut(s) 127
CciI TCATGA 2 cut(s) 589, 1540
Cfr13I GGNCC 2 cut(s) 704, 774
Csp6I GTAC 2 cut(s) 263, 973
CviQI GTAC 2 cut(s) 263, 973
DdeI CTNAG 5 cut(s) 27, 232, 381, 1380, 1494
DpnI GATC 1 cut(s) 1530
DpnII GATC 1 cut(s) 1528
DraIII CACNNNGTG 1 cut(s) 883
EaeI YGGCCR 2 cut(s) 570, 697
Eam1104I CTCTTC 2 cut(s) 436, 657
EarI CTCTTC 2 cut(s) 436, 657
Eco130I CCWWGG 3 cut(s) 484, 700, 903
Eco32I GATATC 2 cut(s) 229, 531
Eco47I GGWCC 2 cut(s) 704, 774
Eco57I CTGAAG 1 cut(s) 1692
EcoRII CCWGG 2 cut(s) 406, 572
EcoRV GATATC 2 cut(s) 229, 531
EcoT14I CCWWGG 3 cut(s) 484, 700, 903
EcoT22I ATGCAT 1 cut(s) 856
ErhI CCWWGG 3 cut(s) 484, 700, 903
FalI AAGNNNNNCTT 8 cut(s) 327, 359, 701, 733, 850, 882, 1332, 1364
FauNDI CATATG 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 3 cut(s) 543, 953, 1080
FokI GGATG 4 cut(s) 782, 929, 1139, 1268
Fsp4HI GCNGC 3 cut(s) 543, 953, 1080
FspBI CTAG 2 cut(s) 56, 863
GluI GCNGC 3 cut(s) 543, 953, 1080
HaeIII GGCC 2 cut(s) 572, 699
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 3 cut(s) 396, 1382, 1486
HinfI GANTC 5 cut(s) 283, 607, 935, 1022, 1544
HphI GGTGA 2 cut(s) 422, 1262
Hpy166II GTNNAC 5 cut(s) 97, 263, 910, 1370, 1660
Hpy188I TCNGA 6 cut(s) 535, 1162, 1237, 1504, 1672, 1694
Hpy188III TCNNGA 9 cut(s) 179, 301, 590, 799, 808, 858, 1107, 1424, 1541
Hpy8I GTNNAC 5 cut(s) 97, 263, 910, 1370, 1660
HpyAV CCTTC 5 cut(s) 203, 558, 572, 1334, 1633
HpyCH4III ACNGT 3 cut(s) 321, 672, 1415
HpyCH4IV ACGT 2 cut(s) 361, 912
HpyCH4V TGCA 9 cut(s) 24, 122, 317, 542, 854, 988, 1055, 1316, 1666
HpyF10VI GCNNNNNNNGC 4 cut(s) 872, 1079, 1445, 1454
HpyF3I CTNAG 5 cut(s) 27, 232, 381, 1380, 1494
HpySE526I ACGT 2 cut(s) 361, 912
Kzo9I GATC 1 cut(s) 1528
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 3 cut(s) 550, 833, 1155
Lsp1109I GCAGC 3 cut(s) 554, 939, 1066
LweI GCATC 7 cut(s) 304, 529, 863, 951, 1117, 1269, 1303
MaeI CTAG 2 cut(s) 56, 863
MaeII ACGT 2 cut(s) 361, 912
MaeIII GTNAC 4 cut(s) 163, 357, 672, 1604
MalI GATC 1 cut(s) 1530
MbiI CCGCTC 1 cut(s) 1566
MboI GATC 1 cut(s) 1528
MboII GAAGA 8 cut(s) 181, 243, 423, 644, 677, 732, 1337, 1685
MflI RGATCY 1 cut(s) 1528
MlsI TGGCCA 2 cut(s) 572, 699
MluNI TGGCCA 2 cut(s) 572, 699
MmeI TCCRAC 2 cut(s) 811, 1281
Mox20I TGGCCA 2 cut(s) 572, 699
Mph1103I ATGCAT 1 cut(s) 856
MroXI GAANNNNTTC 1 cut(s) 1344
MscI TGGCCA 2 cut(s) 572, 699
MseI TTAA 7 cut(s) 131, 270, 458, 630, 915, 1199, 1596
MslI CAYNNNNRTG 1 cut(s) 966
Msp20I TGGCCA 2 cut(s) 572, 699
MspR9I CCNGG 2 cut(s) 408, 574
MvaI CCWGG 2 cut(s) 408, 574
MwoI GCNNNNNNNGC 4 cut(s) 872, 1079, 1445, 1454
NcoI CCATGG 1 cut(s) 903
NdeI CATATG 1 cut(s) 1009
NdeII GATC 1 cut(s) 1528
NlaIV GGNNCC 5 cut(s) 502, 775, 901, 1072, 1151
NmuCI GTSAC 2 cut(s) 357, 1604
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 1 cut(s) 1666
PagI TCATGA 2 cut(s) 589, 1540
PciSI GCTCTTC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 5 cut(s) 283, 607, 935, 1022, 1544
PflMI CCANNNNNTGG 2 cut(s) 713, 1578
PkrI GCNGC 3 cut(s) 544, 954, 1081
Ppu21I YACGTR 1 cut(s) 362
PshBI ATTAAT 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 406, 572
PspGI CCWGG 2 cut(s) 406, 572
PspN4I GGNNCC 5 cut(s) 502, 775, 901, 1072, 1151
PspPI GGNCC 2 cut(s) 704, 774
PsuI RGATCY 1 cut(s) 1528
RsaI GTAC 2 cut(s) 264, 974
RsaNI GTAC 2 cut(s) 263, 973
RseI CAYNNNNRTG 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 7 cut(s) 131, 270, 458, 630, 915, 1199, 1596
SatI GCNGC 3 cut(s) 543, 953, 1080
Sau3AI GATC 1 cut(s) 1528
Sau96I GGNCC 2 cut(s) 704, 774
ScrFI CCNGG 2 cut(s) 408, 574
SfaNI GCATC 7 cut(s) 304, 529, 863, 951, 1117, 1269, 1303
SfcI CTRYAG 1 cut(s) 1357
SinI GGWCC 2 cut(s) 704, 774
SmiMI CAYNNNNRTG 1 cut(s) 966
SsiI CCGC 2 cut(s) 1334, 1564
SspI AATATT 1 cut(s) 103
SspMI CTAG 2 cut(s) 56, 863
StyD4I CCNGG 2 cut(s) 406, 572
StyI CCWWGG 3 cut(s) 484, 700, 903
TaaI ACNGT 3 cut(s) 321, 672, 1415
TaiI ACGT 2 cut(s) 364, 915
TaqI TCGA 2 cut(s) 84, 1065
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 5 cut(s) 283, 607, 935, 1022, 1544
Tru1I TTAA 7 cut(s) 131, 270, 458, 630, 915, 1199, 1596
Tru9I TTAA 7 cut(s) 131, 270, 458, 630, 915, 1199, 1596
TscAI CASTG 4 cut(s) 324, 1222, 1420, 1615
TseFI GTSAC 2 cut(s) 357, 1604
TseI GCWGC 3 cut(s) 542, 952, 1079
Tsp45I GTSAC 2 cut(s) 357, 1604
TspRI CASTG 4 cut(s) 324, 1222, 1420, 1615
Van91I CCANNNNNTGG 2 cut(s) 713, 1578
VpaK11BI GGWCC 2 cut(s) 704, 774
VspI ATTAAT 1 cut(s) 131
XapI RAATTY 5 cut(s) 182, 368, 634, 1135, 1202
XceI RCATGY 1 cut(s) 1666
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 2 cut(s) 56, 863
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.