Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004459

Physical Location & Seq

Genomic Coordinates Forward (+)

27810505 .. 27814956

Gene Structure 4452 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1194100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2979 bp

ATGAAACTAATTCAAACCTCTTCCCATGAAGCCGAAAACCCAATAGAGCTCTCTCTGAGACAAGCCTTTGAACTTCTTGAACCAAAACTGAGGCCCCCTTTTGCATTAACAATTCCAAACCCACAAGAATACTTGTTGCTCAACAAAGCGATCCTTTATGGTGTGTTATGTGAAAGCCATGTTGGAAAAACACATATGAAGCACTTACATGCTATTGTCACTGATGGGTATTTCTGGTTTGTTAGCTTGCTAGTCAAGGTCGTGAATGAACTGTATGTGAAACTTCTTGAACCGGTGAAGTCTCAATTAATTTGGGTTACCAAGGAGTTGATCAATGTTCTAGCGGTAGGTGTTGATGGTTTGTTGGTTTGCTTGTTGAGGCAAATTATTGGAGGGGATTATAGTGATGGAAATCTGTGGCTTTGCTTTGAGATGGTGAGCATTTTTATGACCAAATGGGATTGTTTGTTAGAAGTAGAACCGATGATTTTGACTAGTGGATTGTATACATATCTTCGGTTATTAGCTGATCACTGTAGGTTGTTAAGCAATCCAAAATTAGAGGCATTAAAGCAATTGGAGGTCGATTTTTGCATTAAAGTTTTTAAGGAGAAGTTTCCTTTGTGTTTGAAGATTGGCAGGGATCTTGTTCGACTTCTACAAGATTTGGTTCATTTACCCAAGTTTAGAACAATATGGAAGGATTTGGTATTGAATCGCGGCGAATTTAAAGCTCAGGGATTTTCAGGTATTCCACAACTCTATAACACAAGGACTCCAAGCGAGTATATGTTACTTCGGATTACTCCAGAAATGGAGGCCCATTTACGATTTTTGTTCACTCATGTGAAGTTGGGAAACCAGAAGCGTCATCAGGCATGGTTTTCCAAGAAGTTTCTATTTGAACCAAACAGAGAGACTCTCATAATTGACATTGTTCGCTTTATATGTTGTGCTTACCACCCAACCAATGAAATCATTCAGTCAGATGTCATTCCAAGATGGGCTATTATGGGTTGGCTTTTCAAATCTTGCACAAAGAATTATGTTGAAGCGAATGTGAAACTAGCTCTACTTTATGATTGGCTCTTTTTCAAGGAAAGAGCAGACAATATTATGGATATTGAACCAGCAATGTTATTAATGGTCTATTCTATACCCAGATACATTGATGTAACTCACACTATTCTTGAATTTTTGTTCCTTCTTATGGACAACTATGATGTGGAACATTACGATATCTTAGCTAAAGGTGTGTCATCTTCTTTTAGTGTACTTGTTAAGAGAGGAGTGATTCGTTCACTTGATGTCTTGACCTCTTGTGATGCATTGTCTCCTATATTGAAAGAAAGGCTTGGTAGGTTTTTGTCAGGTAGGAAATTTGAAATCTCTAAAGAGTTGCAACAAGCTTGCCGTCCTGCTGATTTTGTGAGTTTGAACTCTTCATGTATGGAAACTCCATTACCATTAACAGAATGGCAACCAAAATGTAGCCAAGGAGACAAATTTGGTACCAAATCAGCTGAAACTAAAGTTATGATATCAGATGATGCAGCTTCTTCCCATAGTCCATTAGTAGTGGCCAGGGAAGGCCAAGTTCATGATATAGACAATATGATTCATAATCTTGGAGAAGCAATTAAAAATTCAAATCCAATGGCTCTTCGTACTTTGGAAGAACTGTTACTTTTATTTGTGAGTTTTGATGGCCAAGGACCAGAGAGTGGCTTCATTCTGCCTGATGTTTTATCTTCTAAAATAAAAAAGTTGTATGAATTGAGTGGGTCCAAATTATTTTCTCCTCTCAAATTTCATCCAGATAGTCCTGAAAACAGTGATGAAGTTGGGTCTGCCACATCCTTAATTATCCGTATGTTTGTTCTTTCTCAACATCAAAATATGCAAGCTATGCTTCTATTTTGGTTAAAGAATGGTTTTCCAGTTGGAGCAAGGTTATTATTGTATGCATCAAGACTAGCTTATGAGGCACGCATGGTGGGTTTTTTTGGAGATGCCATGGTTGACGTTAATTCTATTACTCCAAGTGATTCAGGCATCCGTCTGCTGCTATATCATGTCAATGGGTACTTTTCTTTTTTGCATGGTAGAACTGAAAACTCATATGAAACCATTGAGTCTATTTCTAAAGCGGAAAAGAAGTTAGTTGCAATGTTAGTCGATGGTGCCTTTGCTGCCTACAAATGCTTCCTTGTGTATTCAAGAACTGCCTTATTCAAGGATGCTGAAAATTCTTTGCCGCAGCTCCTTTTTTCTGACATAATGACTTGTTCTGTGTGGGAGAGGAAGCGATTAAAATTCTTGTTTTCCAGTGTCTATTGTCATCTTTCTGATATGTCCATTGGTGAGGAAGGCATCATCAAGCTACTTGTTGGTCAATTAGATCATGCTGATCTTGTTAATATACAGATTGAGATTGGCTTGAAAAGATTTTCCATTTTTGGTGAGAATACTGAAACAGTTTTCTGCTTAATGAAGAATTCCCTTAATTGGGGTTGTATGGAACAACATAAATTCTGGGGCTTGATTAGATCAGAGCTAGCGGTTTCCACGGTGGAAGTGCAGGGACTACTTTCAAAGTTTTTCTGGTTGGAGAAATTAGATGCAAATGCGAGTGCCATTGCGGTTGAAGGTCTTCTTACACTCTGTAGTTGCTGCCCACCCACGCCTGAGCTTGTTGGGGCTCTCATGTTATTACCTAACAGTGTCTTTCAGGATTTTGCTTCTGCTGTTATGGCTAAATGGGCTGTATCTAATGGCGCCATGCTATTTGAAAGCTTGGCTGAGTTTTCAGAAAAAGTTGAGAGCAAAAATGGGGATCTCGTCATTCATGATTCAGCAGGAATGTTAAACCGTTCTGCCATTTTGTGGTTATTGAAGTATTTTAATGGGAGTGACACTGATATTTCTGGCAACTTATCCTTAAAAACTATGTGCAAAAATGCAATGCGACAAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ints3

Protein Analysis

993

Amino Acids

112.48

Weight (kDa)

6.28

Isoelectric Point (pI)

33.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ints3_N	PF10189	43 - 452	7e-151	Integrator complex subunit 3 N-terminal
HEAT_Ints3_C	PF24566	748 - 938	1.8e-15	Ints3-like, C-terminal HEAT repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14590
fragaria_vesca	FvH4_3g28960
malus_domestica	MD00G1154100.v1.1 MD11G1194100.v1.1 MD15G1397300.v1.1
prunus_persica	Prupe.1G578000_v2.0.a1
pyrus_communis	pycom11g16260 pycom15g35700
rosa_chinensis	RchiOBHm_Chr2g0127281 RchiOBHm_Chr6g0255981 RchiOBHm_Chr6g0267091
rosa_laevigata	RLG00000014039 RLG00000014796 RLG00000034863
rosa_multiflora	Rmu_sc0002938.1_g000050 Rmu_sc0004659.1_g000003 Rmu_ssc0000388.1_g000011
rosa_roxburghii	Rroxscaffold_2G00116300 Rroxscaffold_7G00201030
rosa_rugosa	Rorug02G0275800
rosa_samantha	Rh2BG336100 Rh2DG353800 Rh5CG385400 Rh6AG150700 Rh6BG074000 Rh6BG151500 Rh6CG147100 Rh6DG135600
rosa_wichuraiana	Rw0G004690 Rw2G026530 Rw5G032980 Rw6G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1511
AccB1I	GGYRCC	3 cut(s)	1511, 2183, 2777
AccB7I	CCANNNNNTGG	2 cut(s)	1724, 2886
AccI	GTMKAC	1 cut(s)	506
AccII	CGCG	1 cut(s)	720
AciI	CCGC	6 cut(s)	344, 720, 2150, 2258, 2561, 2642
AclWI	GGATC	3 cut(s)	145, 651, 2844
AcoI	YGGCCR	2 cut(s)	1581, 1708
AcyI	GRCGYC	1 cut(s)	2778
AfaI	GTAC	4 cut(s)	1275, 1513, 1669, 2087
AfiI	CCNNNNNNNGG	5 cut(s)	1210, 1724, 2508, 2509, 2886
AgeI	ACCGGT	1 cut(s)	292
AhlI	ACTAGT	1 cut(s)	494
AjnI	CCWGG	1 cut(s)	1583
AjuI	GAANNNNNNNTTGG	2 cut(s)	165, 197
AleI	CACNNNNGTG	1 cut(s)	845
Alw21I	GWGCWC	1 cut(s)	51
Alw26I	GTCTC	5 cut(s)	52, 306, 911, 1338, 1494
AlwI	GGATC	3 cut(s)	145, 651, 2844
AoxI	GGCC	5 cut(s)	92, 819, 1581, 1591, 1708
ApeKI	GCWGC	5 cut(s)	1553, 2065, 2192, 2260, 2673
AseI	ATTAAT	2 cut(s)	308, 1142
AsiGI	ACCGGT	1 cut(s)	292
Asp700I	GAANNNNTTC	2 cut(s)	978, 2652
Asp718I	GGTACC	1 cut(s)	1511
AspLEI	GCGC	1 cut(s)	2780
AspS9I	GGNCC	4 cut(s)	93, 820, 1715, 1785
AsuHPI	GGTGA	4 cut(s)	307, 448, 2375, 2474
AsuNHI	GCTAGC	1 cut(s)	2557
AvaII	GGWCC	2 cut(s)	1715, 1785
BalI	TGGCCA	2 cut(s)	1583, 1710
BanI	GGYRCC	3 cut(s)	1511, 2183, 2777
BanII	GRGCYC	2 cut(s)	51, 2704
BarI	GAAGNNNNNNTAC	2 cut(s)	1668, 1700
BbsI	GAAGAC	1 cut(s)	2645
Bbv12I	GWGCWC	1 cut(s)	51
BbvI	GCAGC	5 cut(s)	1565, 2052, 2179, 2272, 2660
BccI	CCATC	7 cut(s)	218, 350, 401, 427, 996, 1700, 2174
BceAI	ACGGC	1 cut(s)	1398
BciT130I	CCWGG	1 cut(s)	1585
BclI	TGATCA	2 cut(s)	330, 529
BcoDI	GTCTC	5 cut(s)	52, 306, 911, 1338, 1494
BcuI	ACTAGT	1 cut(s)	494
BfaI	CTAG	6 cut(s)	251, 341, 495, 1067, 1976, 2558
BfmI	CTRYAG	2 cut(s)	535, 2665
BfoI	RGCGCY	1 cut(s)	2781
BisI	GCNGC	7 cut(s)	721, 1554, 2066, 2193, 2258, 2261, 2674
BlsI	GCNGC	7 cut(s)	722, 1555, 2067, 2194, 2259, 2262, 2675
Bme1390I	CCNGG	1 cut(s)	1585
Bme18I	GGWCC	2 cut(s)	1715, 1785
BmgT120I	GGNCC	4 cut(s)	93, 820, 1715, 1785
BmiI	GGNNCC	5 cut(s)	95, 1513, 1786, 2185, 2779
BmrFI	CCNGG	1 cut(s)	1585
BmsI	GCATC	8 cut(s)	1315, 1540, 1976, 2002, 2064, 2230, 2382, 2611
BmtI	GCTAGC	1 cut(s)	2561
BpiI	GAAGAC	1 cut(s)	2645
BplI	GAGNNNNNCTC	2 cut(s)	906, 938
BpmI	CTGGAG	1 cut(s)	792
Bpu10I	CCTNAGC	2 cut(s)	735, 2688
BsaBI	GATNNNNATC	1 cut(s)	411
BsaHI	GRCGYC	1 cut(s)	2778
BsaJI	CCNNGG	6 cut(s)	321, 1495, 1584, 1711, 2016, 2568
BsaWI	WCCGGW	1 cut(s)	292
BsaXI	ACNNNNNCTCC	2 cut(s)	760, 790
Bsc4I	CCNNNNNNNGG	5 cut(s)	1210, 1724, 2508, 2509, 2886
Bse118I	RCCGGY	1 cut(s)	292
Bse1I	ACTGG	2 cut(s)	1940, 2328
Bse3DI	GCAATG	4 cut(s)	1140, 2175, 2637, 2970
Bse8I	GATNNNNATC	1 cut(s)	411
BseBI	CCWGG	1 cut(s)	1585
BseDI	CCNNGG	6 cut(s)	321, 1495, 1584, 1711, 2016, 2568
BseGI	GGATG	4 cut(s)	1813, 1856, 2055, 2245
BseJI	GATNNNNATC	1 cut(s)	411
BseLI	CCNNNNNNNGG	5 cut(s)	1210, 1724, 2508, 2509, 2886
BseMI	GCAATG	4 cut(s)	1140, 2175, 2637, 2970
BseMII	CTCAG	5 cut(s)	47, 80, 749, 2679, 2793
BseNI	ACTGG	2 cut(s)	1940, 2328
BseRI	GAGGAG	2 cut(s)	1302, 1791
BseXI	GCAGC	5 cut(s)	1565, 2052, 2179, 2272, 2660
BsgI	GTGCAG	1 cut(s)	2600
Bsh1236I	CGCG	1 cut(s)	720
BshFI	GGCC	5 cut(s)	94, 821, 1583, 1593, 1710
BshNI	GGYRCC	3 cut(s)	1511, 2183, 2777
BshTI	ACCGGT	1 cut(s)	292
BsiHKAI	GWGCWC	1 cut(s)	51
BsiSI	CCGG	1 cut(s)	293
BslFI	GGGAC	1 cut(s)	2598
BslI	CCNNNNNNNGG	5 cut(s)	1210, 1724, 2508, 2509, 2886
BsmAI	GTCTC	5 cut(s)	52, 306, 911, 1338, 1494
BsmFI	GGGAC	1 cut(s)	2598
BsnI	GGCC	5 cut(s)	94, 821, 1583, 1593, 1710
Bsp1286I	GDGCHC	2 cut(s)	51, 2704
Bsp143I	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
Bsp19I	CCATGG	1 cut(s)	2016
BspACI	CCGC	6 cut(s)	344, 720, 2150, 2258, 2561, 2642
BspANI	GGCC	5 cut(s)	94, 821, 1583, 1593, 1710
BspCNI	CTCAG	5 cut(s)	48, 81, 748, 2680, 2794
BspFNI	CGCG	1 cut(s)	720
BspHI	TCATGA	2 cut(s)	1600, 2848
BspLI	GGNNCC	5 cut(s)	95, 1513, 1786, 2185, 2779
BspOI	GCTAGC	1 cut(s)	2561
BspPI	GGATC	3 cut(s)	145, 651, 2844
BspQI	GCTCTTC	1 cut(s)	1668
BspT107I	GGYRCC	3 cut(s)	1511, 2183, 2777
BsrDI	GCAATG	4 cut(s)	1140, 2175, 2637, 2970
BsrFI	RCCGGY	1 cut(s)	292
BsrI	ACTGG	2 cut(s)	1940, 2328
BssAI	RCCGGY	1 cut(s)	292
BssECI	CCNNGG	6 cut(s)	321, 1495, 1584, 1711, 2016, 2568
BssMI	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
BssNAI	GTATAC	1 cut(s)	507
BssNI	GRCGYC	1 cut(s)	2778
BssT1I	CCWWGG	4 cut(s)	321, 1495, 1711, 2016
Bst1107I	GTATAC	1 cut(s)	507
Bst2UI	CCWGG	1 cut(s)	1585
Bst4CI	ACNGT	8 cut(s)	273, 536, 1683, 1835, 2479, 2572, 2723, 2873
Bst6I	CTCTTC	3 cut(s)	25, 1447, 1668
BstACI	GRCGYC	1 cut(s)	2778
BstAPI	GCANNNNNTGC	1 cut(s)	1909
BstC8I	GCNNGC	5 cut(s)	248, 1411, 1905, 1990, 2559
BstDEI	CTNAG	6 cut(s)	56, 89, 735, 1243, 2688, 2802
BstDSI	CCRYGG	2 cut(s)	2016, 2568
BstEII	GGTNACC	1 cut(s)	316
BstF5I	GGATG	4 cut(s)	1813, 1856, 2055, 2245
BstFNI	CGCG	1 cut(s)	720
BstH2I	RGCGCY	1 cut(s)	2781
BstHHI	GCGC	1 cut(s)	2780
BstKTI	GATC	8 cut(s)	153, 333, 532, 646, 2404, 2413, 2552, 2839
BstMAI	GTCTC	5 cut(s)	52, 306, 911, 1338, 1494
BstMBI	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
BstMWI	GCNNNNNNNGC	5 cut(s)	1909, 1985, 2192, 2753, 2762
BstNI	CCWGG	1 cut(s)	1585
BstNSI	RCATGY	1 cut(s)	212
BstPI	GGTNACC	1 cut(s)	316
BstSCI	CCNGG	1 cut(s)	1583
BstSFI	CTRYAG	2 cut(s)	535, 2665
BstUI	CGCG	1 cut(s)	720
BstV1I	GCAGC	5 cut(s)	1565, 2052, 2179, 2272, 2660
BstV2I	GAAGAC	1 cut(s)	2645
BstX2I	RGATCY	2 cut(s)	643, 2836
BstYI	RGATCY	2 cut(s)	643, 2836
BstZ17I	GTATAC	1 cut(s)	507
BsuRI	GGCC	5 cut(s)	94, 821, 1583, 1593, 1710
BtgI	CCRYGG	2 cut(s)	2016, 2568
BtsCI	GGATG	4 cut(s)	1813, 1856, 2055, 2245
BtsIMutI	CAGTG	6 cut(s)	219, 532, 1840, 2335, 2728, 2916
Cac8I	GCNNGC	5 cut(s)	248, 1411, 1905, 1990, 2559
CciI	TCATGA	2 cut(s)	1600, 2848
CfoI	GCGC	1 cut(s)	2780
Cfr10I	RCCGGY	1 cut(s)	292
Cfr13I	GGNCC	4 cut(s)	93, 820, 1715, 1785
CseI	GACGC	1 cut(s)	857
Csp6I	GTAC	4 cut(s)	1274, 1512, 1668, 2086
CspAI	ACCGGT	1 cut(s)	292
CviQI	GTAC	4 cut(s)	1274, 1512, 1668, 2086
DdeI	CTNAG	6 cut(s)	56, 89, 735, 1243, 2688, 2802
DinI	GGCGCC	1 cut(s)	2779
DpnI	GATC	8 cut(s)	152, 332, 531, 645, 2403, 2412, 2551, 2838
DpnII	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
DraI	TTTAAA	1 cut(s)	730
EaeI	YGGCCR	2 cut(s)	1581, 1708
Eam1104I	CTCTTC	3 cut(s)	25, 1447, 1668
EarI	CTCTTC	3 cut(s)	25, 1447, 1668
Ecl136II	GAGCTC	1 cut(s)	49
Eco130I	CCWWGG	4 cut(s)	321, 1495, 1711, 2016
Eco24I	GRGCYC	2 cut(s)	51, 2704
Eco32I	GATATC	2 cut(s)	1240, 1542
Eco47I	GGWCC	2 cut(s)	1715, 1785
Eco53kI	GAGCTC	1 cut(s)	49
Eco91I	GGTNACC	1 cut(s)	316
EcoICRI	GAGCTC	1 cut(s)	49
EcoO109I	RGGNCCY	1 cut(s)	93
EcoO65I	GGTNACC	1 cut(s)	316
EcoRI	GAATTC	1 cut(s)	2497
EcoRII	CCWGG	1 cut(s)	1583
EcoRV	GATATC	2 cut(s)	1240, 1542
EcoT14I	CCWWGG	4 cut(s)	321, 1495, 1711, 2016
EcoT22I	ATGCAT	2 cut(s)	1330, 1969
EcoT38I	GRGCYC	2 cut(s)	51, 2704
EgeI	GGCGCC	1 cut(s)	2779
EheI	GGCGCC	1 cut(s)	2779
ErhI	CCWWGG	4 cut(s)	321, 1495, 1711, 2016
FaqI	GGGAC	1 cut(s)	2598
FauNDI	CATATG	2 cut(s)	195, 2122
FbaI	TGATCA	2 cut(s)	330, 529
FblI	GTMKAC	1 cut(s)	506
Fnu4HI	GCNGC	7 cut(s)	721, 1554, 2066, 2193, 2258, 2261, 2674
FokI	GGATG	4 cut(s)	1800, 1843, 2042, 2252
FriOI	GRGCYC	2 cut(s)	51, 2704
Fsp4HI	GCNGC	7 cut(s)	721, 1554, 2066, 2193, 2258, 2261, 2674
FspBI	CTAG	6 cut(s)	251, 341, 495, 1067, 1976, 2558
GlaI	GCGC	1 cut(s)	2779
GluI	GCNGC	7 cut(s)	721, 1554, 2066, 2193, 2258, 2261, 2674
GsuI	CTGGAG	1 cut(s)	792
HaeII	RGCGCY	1 cut(s)	2781
HaeIII	GGCC	5 cut(s)	94, 821, 1583, 1593, 1710
HapII	CCGG	1 cut(s)	293
HgaI	GACGC	1 cut(s)	857
HhaI	GCGC	1 cut(s)	2780
Hin1I	GRCGYC	1 cut(s)	2778
Hin6I	GCGC	1 cut(s)	2778
HinP1I	GCGC	1 cut(s)	2778
HincII	GTYRAC	1 cut(s)	2023
HindII	GTYRAC	1 cut(s)	2023
HindIII	AAGCTT	2 cut(s)	1407, 2794
HinfI	GANTC	8 cut(s)	715, 775, 919, 1294, 1618, 2048, 2135, 2852
HpaII	CCGG	1 cut(s)	293
HphI	GGTGA	4 cut(s)	307, 448, 2375, 2474
Hpy166II	GTNNAC	5 cut(s)	507, 840, 1274, 1301, 2023
Hpy188I	TCNGA	8 cut(s)	57, 801, 988, 1546, 2275, 2350, 2554, 2812
Hpy8I	GTNNAC	5 cut(s)	507, 840, 1274, 1301, 2023
HpyAV	CCTTC	5 cut(s)	694, 1214, 1583, 2363, 2642
HpyCH4III	ACNGT	8 cut(s)	273, 536, 1683, 1835, 2479, 2572, 2723, 2873
HpyCH4IV	ACGT	1 cut(s)	2025
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1909, 1985, 2192, 2753, 2762
HpyF3I	CTNAG	6 cut(s)	56, 89, 735, 1243, 2688, 2802
HpySE526I	ACGT	1 cut(s)	2025
Hsp92I	GRCGYC	1 cut(s)	2778
HspAI	GCGC	1 cut(s)	2778
KasI	GGCGCC	1 cut(s)	2777
KpnI	GGTACC	1 cut(s)	1515
Ksp22I	TGATCA	2 cut(s)	330, 529
Kzo9I	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
LguI	GCTCTTC	1 cut(s)	1668
LmnI	GCTCC	2 cut(s)	1946, 2268
Lsp1109I	GCAGC	5 cut(s)	1565, 2052, 2179, 2272, 2660
LweI	GCATC	8 cut(s)	1315, 1540, 1976, 2002, 2064, 2230, 2382, 2611
MaeI	CTAG	6 cut(s)	251, 341, 495, 1067, 1976, 2558
MaeII	ACGT	1 cut(s)	2025
MaeIII	GTNAC	6 cut(s)	217, 316, 792, 1174, 1683, 2912
MalI	GATC	8 cut(s)	152, 332, 531, 645, 2403, 2412, 2551, 2838
MboI	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
MfeI	CAATTG	1 cut(s)	575
MflI	RGATCY	2 cut(s)	643, 2836
MhlI	GDGCHC	2 cut(s)	51, 2704
MlsI	TGGCCA	2 cut(s)	1583, 1710
MluNI	TGGCCA	2 cut(s)	1583, 1710
Mly113I	GGCGCC	1 cut(s)	2778
MlyI	GAGTC	3 cut(s)	769, 913, 2144
MmeI	TCCRAC	3 cut(s)	163, 1924, 2589
Mox20I	TGGCCA	2 cut(s)	1583, 1710
Mph1103I	ATGCAT	2 cut(s)	1330, 1969
MroXI	GAANNNNTTC	2 cut(s)	978, 2652
MscI	TGGCCA	2 cut(s)	1583, 1710
MslI	CAYNNNNRTG	4 cut(s)	207, 446, 845, 2079
Msp20I	TGGCCA	2 cut(s)	1583, 1710
MspA1I	CMGCKG	1 cut(s)	1523
MspI	CCGG	1 cut(s)	293
MspR9I	CCNGG	1 cut(s)	1585
MunI	CAATTG	1 cut(s)	575
MvaI	CCWGG	1 cut(s)	1585
MvnI	CGCG	1 cut(s)	720
MwoI	GCNNNNNNNGC	5 cut(s)	1909, 1985, 2192, 2753, 2762
NarI	GGCGCC	1 cut(s)	2778
NcoI	CCATGG	1 cut(s)	2016
NdeI	CATATG	2 cut(s)	195, 2122
NdeII	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
NheI	GCTAGC	1 cut(s)	2557
NlaIV	GGNNCC	5 cut(s)	95, 1513, 1786, 2185, 2779
NmuCI	GTSAC	2 cut(s)	217, 2912
NsiI	ATGCAT	2 cut(s)	1330, 1969
NspI	RCATGY	1 cut(s)	212
OliI	CACNNNNGTG	1 cut(s)	845
PagI	TCATGA	2 cut(s)	1600, 2848
PciSI	GCTCTTC	1 cut(s)	1668
PdmI	GAANNNNTTC	2 cut(s)	978, 2652
PfeI	GAWTC	5 cut(s)	715, 1294, 1618, 2048, 2852
PflMI	CCANNNNNTGG	2 cut(s)	1724, 2886
PinAI	ACCGGT	1 cut(s)	292
PkrI	GCNGC	7 cut(s)	722, 1555, 2067, 2194, 2259, 2262, 2675
PleI	GAGTC	3 cut(s)	769, 913, 2143
PluTI	GGCGCC	1 cut(s)	2781
PpsI	GAGTC	3 cut(s)	769, 913, 2143
PshBI	ATTAAT	2 cut(s)	308, 1142
Psp124BI	GAGCTC	1 cut(s)	51
Psp6I	CCWGG	1 cut(s)	1583
PspEI	GGTNACC	1 cut(s)	316
PspGI	CCWGG	1 cut(s)	1583
PspN4I	GGNNCC	5 cut(s)	95, 1513, 1786, 2185, 2779
PspPI	GGNCC	4 cut(s)	93, 820, 1715, 1785
PsuI	RGATCY	2 cut(s)	643, 2836
PvuII	CAGCTG	1 cut(s)	1523
RsaI	GTAC	4 cut(s)	1275, 1513, 1669, 2087
RsaNI	GTAC	4 cut(s)	1274, 1512, 1668, 2086
RseI	CAYNNNNRTG	4 cut(s)	207, 446, 845, 2079
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	1 cut(s)	1668
SatI	GCNGC	7 cut(s)	721, 1554, 2066, 2193, 2258, 2261, 2674
Sau3AI	GATC	8 cut(s)	150, 330, 529, 643, 2401, 2410, 2549, 2836
Sau96I	GGNCC	4 cut(s)	93, 820, 1715, 1785
SchI	GAGTC	3 cut(s)	769, 913, 2144
ScrFI	CCNGG	1 cut(s)	1585
SduI	GDGCHC	2 cut(s)	51, 2704
SfaNI	GCATC	8 cut(s)	1315, 1540, 1976, 2002, 2064, 2230, 2382, 2611
SfcI	CTRYAG	2 cut(s)	535, 2665
SfoI	GGCGCC	1 cut(s)	2779
SinI	GGWCC	2 cut(s)	1715, 1785
SmiMI	CAYNNNNRTG	4 cut(s)	207, 446, 845, 2079
SpeI	ACTAGT	1 cut(s)	494
SsiI	CCGC	6 cut(s)	344, 720, 2150, 2258, 2561, 2642
SspDI	GGCGCC	1 cut(s)	2777
SspI	AATATT	1 cut(s)	1114
SspMI	CTAG	6 cut(s)	251, 341, 495, 1067, 1976, 2558
SstI	GAGCTC	1 cut(s)	51
StyD4I	CCNGG	1 cut(s)	1583
StyI	CCWWGG	4 cut(s)	321, 1495, 1711, 2016
TaaI	ACNGT	8 cut(s)	273, 536, 1683, 1835, 2479, 2572, 2723, 2873
TaiI	ACGT	1 cut(s)	2028
TaqI	TCGA	3 cut(s)	585, 652, 2178
TatI	WGTACW	1 cut(s)	1273
TauI	GCSGC	2 cut(s)	723, 2260
TfiI	GAWTC	5 cut(s)	715, 1294, 1618, 2048, 2852
TscAI	CASTG	6 cut(s)	226, 539, 1840, 2335, 2728, 2923
TseFI	GTSAC	2 cut(s)	217, 2912
TseI	GCWGC	5 cut(s)	1553, 2065, 2192, 2260, 2673
Tsp45I	GTSAC	2 cut(s)	217, 2912
TspGWI	ACGGA	2 cut(s)	1859, 2048
TspRI	CASTG	6 cut(s)	226, 539, 1840, 2335, 2728, 2923
Van91I	CCANNNNNTGG	2 cut(s)	1724, 2886
VpaK11BI	GGWCC	2 cut(s)	1715, 1785
VspI	ATTAAT	2 cut(s)	308, 1142
XceI	RCATGY	1 cut(s)	212
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1473
XmiI	GTMKAC	1 cut(s)	506
XmnI	GAANNNNTTC	2 cut(s)	978, 2652
XspI	CTAG	6 cut(s)	251, 341, 495, 1067, 1976, 2558
Zsp2I	ATGCAT	2 cut(s)	1330, 1969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.