Rroxscaffold_2G00116300

Integrator complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004459
Physical Location & Seq
Reverse (-)
43419873 .. 43424948
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116300.1

Sequence Viewer

Length: 1299 bp
ATGGCTTTGAACCTTATTCGGATTGCTTCCCATGAAGCTGAAAACCCCGTGGGCTGCTCTCTGAAGGAGGCCTTTGAGCTTCTTGAACCAAAACTAAGACCCCCATTGGCAATAACAATCCCAACCCCACAGGAATACTTGCTGCTCAACAAGGCTATTTTGTATGGAGTTCTATGTGAAACCCATTTTGCCAAAACGCATATTAAGCATTTACATGCCATTGTCACTGATGGGTATGGCTTGTTTGCTAGTTTGATTGCTAAGGTTGTGAATGAATTGTACCCGAAACTTGTTGACCCGGTGAAGTGCCAGTTGATTTGGGTTACTAAGGAGATGATTGATGTTCATGCTGTAGGCATCCATGGTTTGTTGGTTTGTTTTCTGAGGCAAATTGTTGGTGGGTATTTTAGTGAAGGAAATTTGTGGTTGTGTTTTGAGATCGTGAGCATTTTTTTGACCAAATGGGATTCTCTTTTGGAAGTAGAACCAATGATTTTGACCAGTGGATTGTACACATATCTTCGGCTAGTAGCTGATCACTATAGATTGCTAAGTAATCCAAAGTTGGAAGCGTTAAAGCAATTGGAAATTGATTTCTGCACTAAAGTGTTAAGGGAGCAGTTTTCTGTCTGTTTAAAGATTGGGAGGGATCTTGTTCGGCTCCTACAAGATTTGGTTCATATACCAAACTTTAGAGCTACGTGGAAAGATTTGGTATTGAATCAAGGCAAGTTTAAGACTCTTGGTTTTTCTGTTATTTCGCAGCTCTATAACACAAGGACTTCAAGTCAGTATATTTTACTTCGGATTTCACCAGAAATGGAGACACAGTTGCGATTTTTACTTACTTGCGTGAAGTTGGGGAGTCAAAAGCGTTACCAGGCATGGTTTGCTAAGAAGTTTCTATGTGCACCAAACAGAGAGACTTTGATAGTTGACATTGTCCGTTTTATATGTTGTGCACACCACCCACCAAATGAAATCATACAATCAGATATCATTCCAAGATGGGCTGTTGTAGGTTGGCTATTGAAATATTGCACAAAGAATTATGTCCAAGCCAATGTGAAACTAGCTCTACTTTATGATTGGCTATTCTTTGATGAAAGAGTAGATAATATAATGAATATTGAGCCTGCAATGCTACTGATGGTGTACTCCATACCCAGATACATTGATGTGACTCACACACTTCTTGAGTTTTTGTTCCTTCTTGTAGACAGTTATGATGTGGAACATAAAGATATAATTGTTAGAGGCGTGTCTTCATCTCTTCGTGTACTTGTCAAGGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ints3

Protein Analysis

432

Amino Acids

49.84

Weight (kDa)

7.92

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ints3_N PF10189 45 - 431 2.8e-146 Integrator complex subunit 3 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1218
AclWI GGATC 1 cut(s) 657
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 4 cut(s) 281, 512, 1157, 1281
AgsI TTSAA 5 cut(s) 10, 86, 721, 786, 1033
AhdI GACNNNNNGTC 1 cut(s) 786
AjnI CCWGG 1 cut(s) 879
AleI CACNNNNGTG 1 cut(s) 605
AluBI AGCT 6 cut(s) 38, 79, 533, 698, 766, 1076
AluI AGCT 6 cut(s) 38, 79, 533, 698, 766, 1076
Alw21I GWGCWC 2 cut(s) 913, 964
Alw26I GTCTC 2 cut(s) 818, 917
Alw44I GTGCAC 2 cut(s) 909, 960
AlwI GGATC 1 cut(s) 657
AoxI GGCC 1 cut(s) 69
ApaLI GTGCAC 2 cut(s) 909, 960
ApeKI GCWGC 3 cut(s) 54, 142, 763
ApoI RAATTY 1 cut(s) 418
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 313, 804
BaeGI GKGCMC 2 cut(s) 913, 964
BbsI GAAGAC 1 cut(s) 1257
Bbv12I GWGCWC 2 cut(s) 913, 964
BbvI GCAGC 3 cut(s) 41, 129, 775
BccI CCATC 3 cut(s) 224, 1002, 1144
BciT130I CCWGG 1 cut(s) 881
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 818, 917
BfaI CTAG 3 cut(s) 249, 527, 1073
BfmI CTRYAG 2 cut(s) 351, 541
BisI GCNGC 3 cut(s) 55, 143, 764
BlsI GCNGC 3 cut(s) 56, 144, 765
Bme1390I CCNGG 2 cut(s) 299, 881
BmeRI GACNNNNNGTC 1 cut(s) 786
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 2 cut(s) 299, 881
BmsI GCATC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 1257
Bpu10I CCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 1217
BpuMI CCSGG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 48, 361
BsaXI ACNNNNNCTCC 2 cut(s) 815, 845
Bse1I ACTGG 2 cut(s) 310, 501
Bse3DI GCAATG 1 cut(s) 1146
BseBI CCWGG 1 cut(s) 881
BseDI CCNNGG 2 cut(s) 48, 361
BseGI GGATG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 310, 501
BseSI GKGCMC 2 cut(s) 913, 964
BseXI GCAGC 3 cut(s) 41, 129, 775
BsgI GTGCAG 1 cut(s) 583
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 913, 964
BsiSI CCGG 1 cut(s) 299
BsmAI GTCTC 2 cut(s) 818, 917
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 913, 964
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 438, 535, 649
Bsp19I CCATGG 1 cut(s) 361
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 662
BspPI GGATC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 1146
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 2 cut(s) 310, 501
BssECI CCNNGG 2 cut(s) 48, 361
BssMI GATC 3 cut(s) 438, 535, 649
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 881
Bst4CI ACNGT 2 cut(s) 831, 1223
Bst6I CTCTTC 1 cut(s) 1278
BstAPI GCANNNNNTGC 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 6 cut(s) 95, 261, 327, 383, 551, 894
BstDSI CCRYGG 2 cut(s) 48, 361
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 3 cut(s) 441, 538, 652
BstMAI GTCTC 2 cut(s) 818, 917
BstMBI GATC 3 cut(s) 438, 535, 649
BstMWI GCNNNNNNNGC 3 cut(s) 205, 890, 1141
BstNI CCWGG 1 cut(s) 881
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 297, 879
BstSFI CTRYAG 2 cut(s) 351, 541
BstSLI GKGCMC 2 cut(s) 913, 964
BstV1I GCAGC 3 cut(s) 41, 129, 775
BstV2I GAAGAC 1 cut(s) 1257
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 2 cut(s) 48, 361
BtsCI GGATG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 225, 508
Cac8I GCNNGC 1 cut(s) 1137
Csp6I GTAC 4 cut(s) 280, 511, 1156, 1280
CviAII CATG 5 cut(s) 32, 215, 347, 362, 885
CviQI GTAC 4 cut(s) 280, 511, 1156, 1280
DdeI CTNAG 6 cut(s) 95, 261, 327, 383, 551, 894
DpnI GATC 3 cut(s) 440, 537, 651
DpnII GATC 3 cut(s) 438, 535, 649
DraI TTTAAA 1 cut(s) 636
DriI GACNNNNNGTC 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 1278
Eam1105I GACNNNNNGTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 1278
Eco130I CCWWGG 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 71
Eco32I GATATC 1 cut(s) 997
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 879
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 5 cut(s) 35, 218, 350, 365, 888
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 5 cut(s) 31, 214, 346, 361, 884
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 3 cut(s) 55, 143, 764
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 3 cut(s) 55, 143, 764
FspBI CTAG 3 cut(s) 249, 527, 1073
GluI GCNGC 3 cut(s) 55, 143, 764
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 299
Hin1II CATG 5 cut(s) 35, 218, 350, 365, 888
HincII GTYRAC 2 cut(s) 295, 937
HindII GTYRAC 2 cut(s) 295, 937
HinfI GANTC 5 cut(s) 467, 721, 739, 865, 1183
HpaII CCGG 1 cut(s) 299
HphI GGTGA 2 cut(s) 313, 804
Hpy166II GTNNAC 8 cut(s) 295, 513, 911, 937, 962, 1156, 1219, 1280
Hpy188I TCNGA 5 cut(s) 21, 63, 384, 807, 994
Hpy188III TCNNGA 3 cut(s) 83, 442, 1196
Hpy8I GTNNAC 8 cut(s) 295, 513, 911, 937, 962, 1156, 1219, 1280
HpyAV CCTTC 3 cut(s) 58, 407, 1220
HpyCH4III ACNGT 2 cut(s) 831, 1223
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 5 cut(s) 600, 911, 962, 1041, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 890, 1141
HpyF3I CTNAG 6 cut(s) 95, 261, 327, 383, 551, 894
HpySE526I ACGT 1 cut(s) 701
Hsp92II CATG 5 cut(s) 35, 218, 350, 365, 888
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 3 cut(s) 438, 535, 649
LmnI GCTCC 2 cut(s) 616, 666
LpnPI CCDG 9 cut(s) 116, 312, 323, 514, 828, 866, 893, 1149, 1180
Lsp1109I GCAGC 3 cut(s) 41, 129, 775
LweI GCATC 1 cut(s) 366
MaeI CTAG 3 cut(s) 249, 527, 1073
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 4 cut(s) 223, 322, 875, 1180
MalI GATC 3 cut(s) 440, 537, 651
MboI GATC 3 cut(s) 438, 535, 649
MboII GAAGA 3 cut(s) 512, 1257, 1265
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 2 cut(s) 913, 964
MluCI AATT 7 cut(s) 275, 390, 418, 581, 588, 1048, 1248
MlyI GAGTC 3 cut(s) 733, 874, 1177
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 5 cut(s) 61, 378, 639, 1250, 1285
MseI TTAA 5 cut(s) 204, 575, 611, 635, 735
MslI CAYNNNNRTG 3 cut(s) 213, 605, 1178
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 299, 881
MunI CAATTG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 881
MwoI GCNNNNNNNGC 3 cut(s) 205, 890, 1141
NciI CCSGG 1 cut(s) 299
NcoI CCATGG 1 cut(s) 361
NdeII GATC 3 cut(s) 438, 535, 649
NlaIII CATG 5 cut(s) 35, 218, 350, 365, 888
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 2 cut(s) 223, 1180
NspI RCATGY 1 cut(s) 218
OliI CACNNNNGTG 1 cut(s) 605
PceI AGGCCT 1 cut(s) 71
PfeI GAWTC 2 cut(s) 467, 721
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 3 cut(s) 56, 144, 765
PleI GAGTC 3 cut(s) 733, 873, 1177
PpsI GAGTC 3 cut(s) 733, 873, 1177
Ppu21I YACGTR 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 879
PspGI CCWGG 1 cut(s) 879
PspN4I GGNNCC 1 cut(s) 662
PsuI RGATCY 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 4 cut(s) 281, 512, 1157, 1281
RsaNI GTAC 4 cut(s) 280, 511, 1156, 1280
RseI CAYNNNNRTG 3 cut(s) 213, 605, 1178
SaqAI TTAA 5 cut(s) 204, 575, 611, 635, 735
SatI GCNGC 3 cut(s) 55, 143, 764
Sau3AI GATC 3 cut(s) 438, 535, 649
SchI GAGTC 3 cut(s) 733, 874, 1177
ScrFI CCNGG 2 cut(s) 299, 881
SduI GDGCHC 2 cut(s) 913, 964
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 2 cut(s) 351, 541
SmiMI CAYNNNNRTG 3 cut(s) 213, 605, 1178
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
Sse9I AATT 7 cut(s) 275, 390, 418, 581, 588, 1048, 1248
SseBI AGGCCT 1 cut(s) 71
SspI AATATT 2 cut(s) 1037, 1129
SspMI CTAG 3 cut(s) 249, 527, 1073
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 297, 879
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 2 cut(s) 831, 1223
TaiI ACGT 1 cut(s) 704
TasI AATT 7 cut(s) 275, 390, 418, 581, 588, 1048, 1248
TatI WGTACW 3 cut(s) 510, 1155, 1279
TfiI GAWTC 2 cut(s) 467, 721
Tru1I TTAA 5 cut(s) 204, 575, 611, 635, 735
Tru9I TTAA 5 cut(s) 204, 575, 611, 635, 735
TscAI CASTG 2 cut(s) 232, 508
TseFI GTSAC 2 cut(s) 223, 1180
TseI GCWGC 3 cut(s) 54, 142, 763
Tsp45I GTSAC 2 cut(s) 223, 1180
TspDTI ATGAA 8 cut(s) 48, 288, 335, 668, 993, 1119, 1139, 1257
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 2 cut(s) 232, 508
Tth111I GACNNNGTC 1 cut(s) 941
VneI GTGCAC 2 cut(s) 909, 960
XapI RAATTY 1 cut(s) 418
XceI RCATGY 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 1218
XspI CTAG 3 cut(s) 249, 527, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.