MD15G1397300.v1.1

Integrator complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004459
Physical Location & Seq
Forward (+)
49747083 .. 49750969
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1397300.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGAAACTAATTCAAATCTCTTCCCACGAAGCCAAAAACCCGACAGAGCTCTCTCTCAGGCAAGCCTTTGAACTTTTTGAACCAAAACTGAGGCCCCCTTTTGCATTAACAATTCCAAATCCACAAGAATACTTGTTTCTCAACAAAGCCATCCTTTATGGTGTTTTATGTGAAACCCATGTTGGAAAGGCACATATGAAGCACTTGCATGCTATTGTCACTGATGGGTATTGCTGCTTTGTTAGTTTGCTGGTCGAGGTCGTGAATGAATTGTATGTCAAGCTTCTTGAACCAGTCAAGTCTCGATTGATTTGGGCTACTAAGGAGTTGATCAATGTTCTAGCAGTAGGCGTTGAAGGTTTGTTGGTTTGTTTGTTGAGGCAAATTCTTGGAGGGGATTTTAGTGATGGAAATCTGTGGCTGTGTTTTGAGATGGCGACCATCTTTATGTCGAAATGGGATTACTTGTTACAGGTTGATCCGATGATTTTGACTAGTGGGCTGTATACTTATCTTCGGCTTTTAGCTGATCACTCTAGATTTGTGAGTAATCCAAAATTAGAGGCATTAAAGCAATTGGAAATTGATTTTTGTATTAAAGTTTTAAAGGAGCAGTTTCCTTTGTGTTTGAAGGTTGGCAGGGATCTTGTTCGACTTCTACAAGATTTGGTTCATTTACCCAAGTTTAGAGCAATATGGAAGGATTTGGTATTGAACCAGGGCGAGTTTAAGGCTCGGGGATTTTATGGTATTCCACAGCTCTATAACACAAGGACTCCAAGTGAGTATATGTTACTTCGGATTACTCCAGAAATGGAGGCCCAATTGCGATTTTTACTCACTCATGTGAAGTTGGGAAACCAGAAGCGTCACCAGGCATGGTTTGCCAAGAAGTTTCTATGGGAACCAAACAGGGAGACTCTCATAATTGACATTGTTCGATTTATATGTTGTGCCTACCACCCGACCAACGAAGTCATTCAGTCAGATGTCGTTCCAAGATGGGCTGTTATAGGTTGGCTTTTGAAATCTTGCACGAAGAATTATGTCGAAGCAAATGTGAAATTGGCTCTACTTTATGATTGGCTCTTTTTTGAGGAAAGAGTAGACAATATTATGGATATTGAACCCGCAATGCTATTAATGGTATATTCTATACCCAGATACATTGATATAACTCACACTCTTCTTGAATTTCTATTCCTTCTTATGGAGAACTATGATGTGGAACATTACGATATCTTAGTTAAAGGTGTGTCAACTTCTTTTAGTGTACTTGTTAAGAGAGGAGTGATTTGTTCACTTGATGTCTTGACCTCTTGTGATGCACTGTCTCCTATATTGCAACAAGCTCGACAGGTTTTTGTCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ints3

Protein Analysis

458

Amino Acids

53.0

Weight (kDa)

7.16

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ints3_N PF10189 43 - 450 8.3e-149 Integrator complex subunit 3 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 506, 1107
AciI CCGC 1 cut(s) 1131
AclWI GGATC 2 cut(s) 473, 651
AcsI RAATTY 2 cut(s) 384, 1193
AfaI GTAC 1 cut(s) 1275
AfiI CCNNNNNNNGG 1 cut(s) 1210
AhlI ACTAGT 1 cut(s) 494
AjnI CCWGG 2 cut(s) 717, 873
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 5 cut(s) 49, 283, 527, 760, 1352
AluI AGCT 5 cut(s) 49, 283, 527, 760, 1352
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 3 cut(s) 306, 911, 1338
AlwI GGATC 2 cut(s) 473, 651
Ama87I CYCGRG 1 cut(s) 735
AoxI GGCC 2 cut(s) 92, 819
ApeKI GCWGC 1 cut(s) 234
ApoI RAATTY 2 cut(s) 384, 1193
AseI ATTAAT 1 cut(s) 1142
Asp700I GAANNNNTTC 1 cut(s) 978
AspS9I GGNCC 2 cut(s) 93, 820
AsuHPI GGTGA 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 735
BanII GRGCYC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 221
BccI CCATC 6 cut(s) 158, 218, 401, 427, 449, 996
BcgI CGANNNNNNTGC 2 cut(s) 1334, 1368
BciT130I CCWGG 2 cut(s) 719, 875
BclI TGATCA 2 cut(s) 330, 529
BcoDI GTCTC 3 cut(s) 306, 911, 1338
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 3 cut(s) 341, 495, 537
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 2 cut(s) 719, 875
BmeT110I CYCGRG 1 cut(s) 735
BmgT120I GGNCC 2 cut(s) 93, 820
BmiI GGNNCC 2 cut(s) 95, 906
BmrFI CCNGG 2 cut(s) 719, 875
BmsI GCATC 1 cut(s) 1315
BpmI CTGGAG 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 760, 790
Bsc4I CCNNNNNNNGG 1 cut(s) 1210
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 1140
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 2 cut(s) 719, 875
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 1 cut(s) 150
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 1210
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 2 cut(s) 70, 80
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 1302
BseXI GCAGC 1 cut(s) 221
BshFI GGCC 2 cut(s) 94, 821
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 735
BslI CCNNNNNNNGG 1 cut(s) 1210
BsmAI GTCTC 3 cut(s) 306, 911, 1338
BsnI GGCC 2 cut(s) 94, 821
BsoBI CYCGRG 1 cut(s) 735
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 4 cut(s) 330, 478, 529, 643
BspACI CCGC 1 cut(s) 1131
BspANI GGCC 2 cut(s) 94, 821
BspCNI CTCAG 2 cut(s) 69, 81
BspLI GGNNCC 2 cut(s) 95, 906
BspPI GGATC 2 cut(s) 473, 651
BsrDI GCAATG 1 cut(s) 1140
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 4 cut(s) 330, 478, 529, 643
BssNAI GTATAC 1 cut(s) 507
Bst1107I GTATAC 1 cut(s) 507
Bst2UI CCWGG 2 cut(s) 719, 875
Bst4CI ACNGT 1 cut(s) 1332
Bst6I CTCTTC 2 cut(s) 25, 1191
BstAPI GCANNNNNTGC 1 cut(s) 884
BstC8I GCNNGC 2 cut(s) 63, 210
BstDEI CTNAG 4 cut(s) 56, 89, 321, 1243
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 4 cut(s) 333, 481, 532, 646
BstMAI GTCTC 3 cut(s) 306, 911, 1338
BstMBI GATC 4 cut(s) 330, 478, 529, 643
BstMWI GCNNNNNNNGC 1 cut(s) 884
BstNI CCWGG 2 cut(s) 719, 875
BstNSI RCATGY 1 cut(s) 212
BstSCI CCNGG 2 cut(s) 717, 873
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BstZ17I GTATAC 1 cut(s) 507
BsuRI GGCC 2 cut(s) 94, 821
BtsCI GGATG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 219, 1328
Cac8I GCNNGC 2 cut(s) 63, 210
Cfr13I GGNCC 2 cut(s) 93, 820
CseI GACGC 1 cut(s) 857
Csp6I GTAC 1 cut(s) 1274
CviAII CATG 4 cut(s) 179, 209, 845, 879
CviQI GTAC 1 cut(s) 1274
DdeI CTNAG 4 cut(s) 56, 89, 321, 1243
DpnI GATC 4 cut(s) 332, 480, 531, 645
DpnII GATC 4 cut(s) 330, 478, 529, 643
DraI TTTAAA 1 cut(s) 606
Eam1104I CTCTTC 2 cut(s) 25, 1191
EarI CTCTTC 2 cut(s) 25, 1191
Ecl136II GAGCTC 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 51
Eco32I GATATC 1 cut(s) 1240
Eco53kI GAGCTC 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 735
EcoICRI GAGCTC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 93
EcoRII CCWGG 2 cut(s) 717, 873
EcoRV GATATC 1 cut(s) 1240
EcoT38I GRGCYC 1 cut(s) 51
FaeI CATG 4 cut(s) 182, 212, 848, 882
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 4 cut(s) 178, 208, 844, 878
FauI CCCGC 1 cut(s) 1138
FauNDI CATATG 1 cut(s) 195
FbaI TGATCA 2 cut(s) 330, 529
FblI GTMKAC 2 cut(s) 506, 1107
Fnu4HI GCNGC 1 cut(s) 235
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 3 cut(s) 341, 495, 537
GluI GCNGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 2 cut(s) 94, 821
HgaI GACGC 1 cut(s) 857
Hin1II CATG 4 cut(s) 182, 212, 848, 882
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 775, 919
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 5 cut(s) 507, 1108, 1260, 1274, 1301
Hpy188I TCNGA 3 cut(s) 483, 801, 988
Hpy188III TCNNGA 7 cut(s) 262, 287, 303, 537, 809, 1190, 1312
Hpy8I GTNNAC 5 cut(s) 507, 1108, 1260, 1274, 1301
HpyAV CCTTC 4 cut(s) 350, 625, 694, 1214
HpyCH4III ACNGT 1 cut(s) 1332
HpyCH4V TGCA 5 cut(s) 104, 208, 1035, 1328, 1345
HpyF10VI GCNNNNNNNGC 1 cut(s) 884
HpyF3I CTNAG 4 cut(s) 56, 89, 321, 1243
Hsp92II CATG 4 cut(s) 182, 212, 848, 882
Ksp22I TGATCA 2 cut(s) 330, 529
Kzo9I GATC 4 cut(s) 330, 478, 529, 643
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 1315
MaeI CTAG 3 cut(s) 341, 495, 537
MaeIII GTNAC 4 cut(s) 217, 468, 792, 869
MalI GATC 4 cut(s) 332, 480, 531, 645
MboI GATC 4 cut(s) 330, 478, 529, 643
MboII GAAGA 4 cut(s) 12, 506, 1051, 1178
MfeI CAATTG 2 cut(s) 575, 824
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 1 cut(s) 51
MlyI GAGTC 2 cut(s) 769, 913
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 9 cut(s) 84, 250, 372, 386, 556, 811, 1090, 1280, 1327
MroXI GAANNNNTTC 1 cut(s) 978
MseI TTAA 8 cut(s) 107, 569, 597, 605, 729, 1142, 1248, 1281
MslI CAYNNNNRTG 3 cut(s) 207, 446, 845
MspR9I CCNGG 2 cut(s) 719, 875
MunI CAATTG 2 cut(s) 575, 824
MvaI CCWGG 2 cut(s) 719, 875
MwoI GCNNNNNNNGC 1 cut(s) 884
NdeI CATATG 1 cut(s) 195
NdeII GATC 4 cut(s) 330, 478, 529, 643
NlaIII CATG 4 cut(s) 182, 212, 848, 882
NlaIV GGNNCC 2 cut(s) 95, 906
NmuCI GTSAC 2 cut(s) 217, 869
NspI RCATGY 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 845
PaeI GCATGC 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 978
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 2 cut(s) 769, 913
PpsI GAGTC 2 cut(s) 769, 913
PshBI ATTAAT 1 cut(s) 1142
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 717, 873
PspGI CCWGG 2 cut(s) 717, 873
PspN4I GGNNCC 2 cut(s) 95, 906
PspPI GGNCC 2 cut(s) 93, 820
PsuI RGATCY 1 cut(s) 643
RsaI GTAC 1 cut(s) 1275
RsaNI GTAC 1 cut(s) 1274
RseI CAYNNNNRTG 3 cut(s) 207, 446, 845
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 8 cut(s) 107, 569, 597, 605, 729, 1142, 1248, 1281
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 4 cut(s) 330, 478, 529, 643
Sau96I GGNCC 2 cut(s) 93, 820
SchI GAGTC 2 cut(s) 769, 913
ScrFI CCNGG 2 cut(s) 719, 875
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 1 cut(s) 1315
SmiMI CAYNNNNRTG 3 cut(s) 207, 446, 845
SpeI ACTAGT 1 cut(s) 494
SphI GCATGC 1 cut(s) 212
SsiI CCGC 1 cut(s) 1131
SspI AATATT 1 cut(s) 1114
SspMI CTAG 3 cut(s) 341, 495, 537
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 717, 873
TaaI ACNGT 1 cut(s) 1332
TaqI TCGA 7 cut(s) 255, 304, 452, 652, 940, 1050, 1354
TatI WGTACW 1 cut(s) 1273
Tru1I TTAA 8 cut(s) 107, 569, 597, 605, 729, 1142, 1248, 1281
Tru9I TTAA 8 cut(s) 107, 569, 597, 605, 729, 1142, 1248, 1281
TscAI CASTG 2 cut(s) 226, 1335
TseFI GTSAC 2 cut(s) 217, 869
TseI GCWGC 1 cut(s) 234
Tsp45I GTSAC 2 cut(s) 217, 869
TspDTI ATGAA 4 cut(s) 17, 212, 282, 662
TspRI CASTG 2 cut(s) 226, 1335
VspI ATTAAT 1 cut(s) 1142
XapI RAATTY 2 cut(s) 384, 1193
XbaI TCTAGA 1 cut(s) 536
XceI RCATGY 1 cut(s) 212
XmiI GTMKAC 2 cut(s) 506, 1107
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 3 cut(s) 341, 495, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.