Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003360

Physical Location & Seq

Genomic Coordinates Forward (+)

40272546 .. 40278466

Gene Structure 5921 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1173700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGGAAACTCGGAGCCGGAAGCGGGCGGAGGCCACCTCAGCAGCCCCTTCTTCTTCTTCGTCTGGTCGCACCACTCGTTCTTCCAAGCGCGCTCGTCTCTCTGCTTCTTCATTGTCTTCTGTTAGCCCCACTCCGATTACGACCCGGTCCCGCGCCTCTCGCACCCAAAACGAACCTGCACCCGCCGCACCAATGGACCCCACGAACGAATCGTCCGGCTCGCGTGGCCGCCGCAACAAGAGTTCCGATAAGGATGGCTCGGATAAAGGGAAGGAGAAGGAGCATGAGGTTAGGGTTAGGGATAGGGAGAGAGAGAGGGAGAGGGAAAGAGAGAGGGAAGCTGAGAGGAACCTAGGGTTGAATATGGATGCAGGTGGAACTGGCGACGACGATGACAATGATAGCGAAGGCGGTGTTGGAATTTTGCACCAGAACTTGACTTCAGCGAGTAGTGCCCTTCAGGGGCTTCTTAGGAAGATCGGTGCTGGACTGGATGACCTGCTTCCTTCTTCGGCTATGGGTTCGGCTTCGTCCTCGCACCAGAGCGGGAGGCTGAAGAAGATTTTATCTGGGTTAAGAGCTGATGGGGAAGAAGGGAAACAGGTGGAAGCTTTGACTCAGCTGTGTGAAATGCTTTCAATTGGAACTGAGGAGTCGTTGAGCACATTTTCGGTTGATTCATTTGTGCCTGTACTTGTGGGATTGCTAAATCATGAGAGCAATCCAGATATTATGCTTCTTGCAGCTAGAGCACTCACTCATCTTTGCGATGTGCTTCCATCGTCTTGTGCCGCCGTGGTACATTATGGTGCTGTTTCTTGCTTTTGTGCAAGGTTGTTGACGATTGAGTACATGGACTTGGCAGAGCAGTCACTGCAAGCTCTCAAGAAAATATCACAAGAACACCCAACTGCCTGTCTTCGAGCTGGTGCTCTTATGGCGGTGCTTTCTTATCTGGATTTTTTCTCCACAGGAGTCCAGCGTGTGGCATTATCAACTGCTGCGAATATGTGCAAGAAACTTCCTTTAGATGCAGCTGACTTTGTGATGGAAGCTGTCCCATTGTTGACAAACCTTCTGCAGTATCATGATGCAAAGGTTTTGGAACATGCTTCTGTATGTCTGACACGAATTGCTGAAGCATTTGCATCATCTCCTGACAAACTTGACGAATTATGCAACCATGGGCTAGTTACTCAAGCTGCGTCCCTTATTTCCACCAGCAATTCTGGAGGTGGTCAGTCAACTCTAAGTACACCTACTTATACTGGTTTAATTCGACTACTATCAACCTGTGCAAGTGGTTCCCCATTAGGGTCTAAAACTTTGCTTCTTCTTGGGATCAGTGGAATTCTTAAAGATGTACTTTCAGGTTCTGGAATTTCTTCTAGCACATCAGTTTCTCCTGCTTTAAGTAAGCCACCAGAGCAGATCTTTGAGATTGTCAATCTGGCAAATGAGCTACTTCCTCCATTGCCACAAGGAACCATTTCCATTCCATCCAGCTTCAATGTATTTATGAAAGGTCCTGTTGTGAAAAAGTCGTCTGCTAGCGGCTCTGGGAAACAAGAAGACACCAATGGAACTGGTTCTGAGGTTTCAGCTCGTGAGAAATTATTGAATGAACAGCCTGGGCTTCTCCAGCAATTTGGAATGGATCTCCTTCCTGTTTTAATACAGATTTATGGTTCTAGCGTTAATGGCCCTGTTCGCCACAAATGTCTTTCAGTAATTGGGAAATTAATGTACTTCAGCAATGCAGAGATGATACAGTCTTTATTAAGTTCAACAAATATTTCAAGTTTCTTAGCCGGTGTCTTAGCATGGAAAGATCCGCATGTCTTGGTTCCTGCTCTCCAAATTGCTGAGATACTCATGGAGAAGCTTCCCAATACTTTCTCCAAGGTGTTTGTGAGAGAAGGTGTTGTTCATGCCGTGGACCAACTTATCTTACCTGGTACTCCAAATTCAGTTCCTGTCCCAGTATCTTCTGCTGAAAAGGATAGTGATCCTGTTCCTGGAACATCATCACGTTCTAGGCGTTATAGACGACGCAACAGTAACTCAAATCCAGATGGAAATTCATTGGAAGAAACTAAGATTCCCCCTTCAGCAAATATTGGTTCACCTCCAAGTTCAGTGGAGATTCCTACTGTTAACTCCAGCCTTCGAATGGCTGTCAGTGCATGTGCTAAAGCTTTTAAAGACAAGTACTTCCCTTCAGATCCTGGGGCTGTTGAAGTTGGAGTTACAGATGATCTATTACATTTAAAGAATCTATGCATGAAGTTGAATGCAGGTGTTGATGACCAAAAAACGAAAGCAAAGGGAAAGTCGAAAGCTTTTGGGTCTCGCTTGGTTGATAGTTCTGCAAGTAAGGAGGAGTACATGATTGGTGTGGTATCTGAGATGCTAGCCGAACTAAGCAAGGGGGATGGTGTATCCACTTTTGAGTTCATTGGTAGTGGTGTTGTTGCAGCATTGCTCAACTATTTTTCTTATGGGTACTTCTCCAAGGAGAGGATTTCGGAAGCTAATTTGCCCAAGCTTCATGAGCAAGCACTGAGAAGATTTAAGTCATTTGTTGCTGTTGCTCTTCCTTTCAGTATTAATGAAGGAAGTGTAGCACCAATGACTGTCCTCGTTCAGAAGCTCCAAAATGCTTTGTCCTCCTTAGAGCGTTTTCCGGTTGTGCTGAGTCATTCATCTAGGTCATCCACTGGGAGCGCACGCCTCTCCTCTGGACTTAGTGCATTATCTCAGCCCTTTAAGTTGCGTCTTTGTCGAGCACAAGGAGAAAAGACCATCCGAGACTATTCATCGAACGTTGTTCTGATTGATCCATTAGCAAGTTTAGCTGCTGTAGAAGAGTTTCTTTGGCCTCGAGTACAGCGAGGGGAATCTGGTCAGAAGCCTGCTGCCTCTGCTGGAAACTCGGAATCTGGAACTACACCTACAGGTGCTGGTGCTTCATCTCTGTCTACTTCAAATCCTGCTTCCACCACTCGTCGCCATTCAACGCGATCCAGAACATCTGTGAATATAGGAGATACAGCTAGGAGGGAACCATCACAAGAGAAGAGCACAAGCTCATCAAAAGGGAAGGGTAAAGCTGTTTTGAAGCCTTCTCAGGAGGAAGCAAGAGGACCTCAAACGAGAAATGCAGCTCGTAGACGGGCAGCTCTTGATAAAGATGTTCAAATGAAGCCCACAAATGGGGACACTACTTCTGAGGATGAGGAATTGGATATATCGCCTGTGTTGAGATGGATGAATTGGTTATTGATGAAGATGATGATATCTCAGATGACGAAGATGATGACCATGATGTATGTTCTAAGAGATGACTCTCTTCCCGTTTGCATGCCGGATAAAGTACACGATGTCAAATTGGGAGACTCAACTGAGGATGCTACTGTAGCTTCAGCCACAAGTGACAATCATACTAACCCAGCTTCTGGCTCTAGTAGCAGAGCTGCTACAGGTAGAGGCTCAGATTCTGCTGAAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006464 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0030154 GO:0032446 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0036211 GO:0042023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:0140096 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1171

Amino Acids

124.63

Weight (kDa)

8.32

Isoelectric Point (pI)

50.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	177 - 308	1.3e-50	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38600 AT4G38600 AT4G38600
fragaria_vesca	FvH4_2g01290
malus_domestica	MD00G1173700.v1.1 MD00G1173800.v1.1 MD00G1173900.v1.1 MD05G1120900.v1.1 MD10G1095300.v1.1
prunus_persica	Prupe.8G164700_v2.0.a1
pyrus_communis	pycom05g11490 pycom05g11500 pycom10g10610 pycom10g10620
rosa_chinensis	RchiOBHm_Chr6g0251951 RchiOBHm_Chr6g0252001
rosa_laevigata	RLG00000015410 RLG00000015417
rosa_multiflora	Rmu_sc0002522.1_g000052 Rmu_sc0002522.1_g000062 Rmu_ssc0000303.1_g000016
rosa_roxburghii	Rroxscaffold_7G00216270
rosa_rugosa	Rorug05G0505300.1
rosa_samantha	Rh6AG016800 Rh6AG017300 Rh6AG017400 Rh6AG018000 Rh6AG018100 Rh6AG018900 Rh6AG019000 Rh6CG011400 Rh6DG012700
rosa_wichuraiana	Rw6G000110 Rw6G000120 Rw6G000150 Rw6G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	362, 2288
Acc36I	ACCTGC	4 cut(s)	184, 362, 507, 2288
AccB7I	CCANNNNNTGG	2 cut(s)	985, 3458
AccBSI	CCGCTC	1 cut(s)	546
AccI	GTMKAC	2 cut(s)	2981, 3172
AccII	CGCG	4 cut(s)	90, 153, 223, 3022
AclI	AACGTT	1 cut(s)	2826
AclWI	GGATC	7 cut(s)	1349, 1665, 1826, 2003, 2219, 2834, 3018
AcoI	YGGCCR	1 cut(s)	226
AcsI	RAATTY	5 cut(s)	420, 1350, 1380, 1966, 2080
AcuI	CTGAAG	8 cut(s)	426, 443, 575, 1158, 1735, 2094, 2205, 3408
AjnI	CCWGG	4 cut(s)	1630, 1954, 2017, 2227
AjuI	GAANNNNNNNTTGG	2 cut(s)	399, 431
AloI	GAACNNNNNNTCC	2 cut(s)	197, 229
Alw21I	GWGCWC	5 cut(s)	665, 754, 934, 2791, 3086
Alw26I	GTCTC	4 cut(s)	101, 2355, 2805, 3390
AlwI	GGATC	7 cut(s)	1349, 1665, 1826, 2003, 2219, 2834, 3018
AlwNI	CAGNNNCTG	7 cut(s)	874, 1376, 1976, 2228, 2963, 3458, 3500
Ama87I	CYCGRG	1 cut(s)	2883
AoxI	GGCC	4 cut(s)	30, 226, 1702, 2879
ApoI	RAATTY	5 cut(s)	420, 1350, 1380, 1966, 2080
ArsI	GACNNNNNNTTYG	4 cut(s)	197, 229, 2318, 2350
AseI	ATTAAT	2 cut(s)	1742, 2610
Asp700I	GAANNNNTTC	4 cut(s)	1384, 1489, 1588, 2871
AspA2I	CCTAGG	1 cut(s)	352
AspLEI	GCGC	4 cut(s)	90, 92, 155, 2729
AspS9I	GGNCC	6 cut(s)	147, 196, 1526, 1703, 1939, 3146
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	1 cut(s)	2118
AsuII	TTCGAA	1 cut(s)	2170
AsuNHI	GCTAGC	2 cut(s)	1550, 2413
AvaI	CYCGRG	1 cut(s)	2883
AvaII	GGWCC	5 cut(s)	147, 196, 1526, 1939, 3146
AvrII	CCTAGG	1 cut(s)	352
BaeGI	GKGCMC	1 cut(s)	457
BauI	CACGAG	1 cut(s)	1605
BbsI	GAAGAC	3 cut(s)	108, 911, 1578
Bbv12I	GWGCWC	5 cut(s)	665, 754, 934, 2791, 3086
BbvCI	CCTCAGC	1 cut(s)	37
BceAI	ACGGC	2 cut(s)	779, 1919
BciT130I	CCWGG	4 cut(s)	1632, 1956, 2019, 2229
BciVI	GTATCC	1 cut(s)	2452
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	4 cut(s)	101, 2355, 2805, 3390
BfmI	CTRYAG	5 cut(s)	1079, 2862, 2955, 3417, 3480
BfuAI	ACCTGC	4 cut(s)	184, 362, 507, 2288
BfuI	GTATCC	1 cut(s)	2452
BglII	AGATCT	1 cut(s)	1431
BlnI	CCTAGG	1 cut(s)	352
BmcAI	AGTACT	1 cut(s)	2213
Bme1390I	CCNGG	5 cut(s)	145, 1632, 1956, 2019, 2229
Bme18I	GGWCC	5 cut(s)	147, 196, 1526, 1939, 3146
BmeT110I	CYCGRG	1 cut(s)	2883
BmgT120I	GGNCC	6 cut(s)	147, 196, 1526, 1703, 1939, 3146
BmiI	GGNNCC	8 cut(s)	14, 149, 198, 350, 1306, 1486, 1848, 3066
BmrFI	CCNGG	5 cut(s)	145, 1632, 1956, 2019, 2229
BmrI	ACTGGG	2 cut(s)	1976, 2730
BmsI	GCATC	6 cut(s)	358, 1021, 1081, 1157, 2400, 3400
BmtI	GCTAGC	2 cut(s)	1554, 2417
BmuI	ACTGGG	2 cut(s)	1976, 2730
BpiI	GAAGAC	3 cut(s)	108, 911, 1578
BplI	GAGNNNNNCTC	2 cut(s)	20, 52
BpmI	CTGGAG	3 cut(s)	1251, 1625, 2146
Bpu10I	CCTNAGC	1 cut(s)	37
Bpu14I	TTCGAA	1 cut(s)	2170
BpuEI	CTTGAG	2 cut(s)	869, 1182
BpuMI	CCSGG	1 cut(s)	145
BsaBI	GATNNNNATC	1 cut(s)	2007
BsaI	GGTCTC	1 cut(s)	2355
BsaJI	CCNNGG	8 cut(s)	352, 795, 1183, 1631, 1902, 1935, 2228, 2514
BsaWI	WCCGGW	1 cut(s)	2686
Bse118I	RCCGGY	1 cut(s)	1811
Bse1I	ACTGG	6 cut(s)	385, 495, 1273, 1591, 1982, 2725
Bse3DI	GCAATG	3 cut(s)	1472, 1762, 2480
Bse8I	GATNNNNATC	1 cut(s)	2007
BseBI	CCWGG	4 cut(s)	1632, 1956, 2019, 2229
BseDI	CCNNGG	8 cut(s)	352, 795, 1183, 1631, 1902, 1935, 2228, 2514
BseJI	GATNNNNATC	1 cut(s)	2007
BseMI	GCAATG	3 cut(s)	1472, 1762, 2480
BseNI	ACTGG	6 cut(s)	385, 495, 1273, 1591, 1982, 2725
BsePI	GCGCGC	1 cut(s)	88
BseRI	GAGGAG	3 cut(s)	665, 2396, 2728
BseSI	GKGCMC	1 cut(s)	457
BseYI	CCCAGC	1 cut(s)	3451
BsgI	GTGCAG	1 cut(s)	162
Bsh1236I	CGCG	4 cut(s)	90, 153, 223, 3022
BshFI	GGCC	4 cut(s)	32, 228, 1704, 2881
BsiHKAI	GWGCWC	5 cut(s)	665, 754, 934, 2791, 3086
BsiHKCI	CYCGRG	1 cut(s)	2883
BsiSI	CCGG	6 cut(s)	16, 145, 216, 1812, 2687, 3368
BslFI	GGGAC	5 cut(s)	133, 1043, 1192, 1964, 3233
BsmAI	GTCTC	4 cut(s)	101, 2355, 2805, 3390
BsmBI	CGTCTC	1 cut(s)	101
BsmFI	GGGAC	5 cut(s)	133, 1043, 1192, 1964, 3233
BsmI	GAATGC	1 cut(s)	2299
BsnI	GGCC	4 cut(s)	32, 228, 1704, 2881
Bso31I	GGTCTC	1 cut(s)	2355
BsoBI	CYCGRG	1 cut(s)	2883
Bsp119I	TTCGAA	1 cut(s)	2170
Bsp1286I	GDGCHC	6 cut(s)	457, 665, 754, 934, 2791, 3086
Bsp19I	CCATGG	1 cut(s)	1183
BspANI	GGCC	4 cut(s)	32, 228, 1704, 2881
BspFNI	CGCG	4 cut(s)	90, 153, 223, 3022
BspHI	TCATGA	3 cut(s)	712, 1087, 2551
BspLI	GGNNCC	8 cut(s)	14, 149, 198, 350, 1306, 1486, 1848, 3066
BspMAI	CTGCAG	1 cut(s)	1083
BspMI	ACCTGC	4 cut(s)	184, 362, 507, 2288
BspOI	GCTAGC	2 cut(s)	1554, 2417
BspPI	GGATC	7 cut(s)	1349, 1665, 1826, 2003, 2219, 2834, 3018
BspQI	GCTCTTC	2 cut(s)	2601, 3074
BspT104I	TTCGAA	1 cut(s)	2170
BspTNI	GGTCTC	1 cut(s)	2355
BsrBI	CCGCTC	1 cut(s)	546
BsrDI	GCAATG	3 cut(s)	1472, 1762, 2480
BsrFI	RCCGGY	1 cut(s)	1811
BsrI	ACTGG	6 cut(s)	385, 495, 1273, 1591, 1982, 2725
BssAI	RCCGGY	1 cut(s)	1811
BssECI	CCNNGG	8 cut(s)	352, 795, 1183, 1631, 1902, 1935, 2228, 2514
BssHII	GCGCGC	1 cut(s)	88
BssSI	CACGAG	1 cut(s)	1605
BssT1I	CCWWGG	4 cut(s)	352, 1183, 1902, 2514
Bst2BI	CACGAG	1 cut(s)	1605
Bst2UI	CCWGG	4 cut(s)	1632, 1956, 2019, 2229
Bst4CI	ACNGT	5 cut(s)	1773, 2060, 2155, 2638, 3418
Bst6I	CTCTTC	4 cut(s)	2601, 2862, 3074, 3357
BstAPI	GCANNNNNTGC	1 cut(s)	874
BstBI	TTCGAA	1 cut(s)	2170
BstDSI	CCRYGG	3 cut(s)	795, 1183, 1935
BstFNI	CGCG	4 cut(s)	90, 153, 223, 3022
BstHHI	GCGC	4 cut(s)	90, 92, 155, 2729
BstMAI	GTCTC	4 cut(s)	101, 2355, 2805, 3390
BstNI	CCWGG	4 cut(s)	1632, 1956, 2019, 2229
BstNSI	RCATGY	4 cut(s)	1112, 1841, 2190, 3367
BstSCI	CCNGG	5 cut(s)	143, 1630, 1954, 2017, 2227
BstSFI	CTRYAG	5 cut(s)	1079, 2862, 2955, 3417, 3480
BstSLI	GKGCMC	1 cut(s)	457
BstUI	CGCG	4 cut(s)	90, 153, 223, 3022
BstV2I	GAAGAC	3 cut(s)	108, 911, 1578
BstX2I	RGATCY	4 cut(s)	1431, 1657, 1831, 2224
BstXI	CCANNNNNNTGG	1 cut(s)	1649
BstYI	RGATCY	4 cut(s)	1431, 1657, 1831, 2224
BsuI	GTATCC	1 cut(s)	2452
BsuRI	GGCC	4 cut(s)	32, 228, 1704, 2881
BtgI	CCRYGG	3 cut(s)	795, 1183, 1935
BtgZI	GCGATG	1 cut(s)	783
BtsI	GCAGTG	1 cut(s)	872
BtsIMutI	CAGTG	6 cut(s)	872, 1351, 2145, 2188, 2561, 2718
BveI	ACCTGC	4 cut(s)	184, 362, 507, 2288
CaiI	CAGNNNCTG	7 cut(s)	874, 1376, 1976, 2228, 2963, 3458, 3500
CciI	TCATGA	3 cut(s)	712, 1087, 2551
CfoI	GCGC	4 cut(s)	90, 92, 155, 2729
Cfr10I	RCCGGY	1 cut(s)	1811
Cfr13I	GGNCC	6 cut(s)	147, 196, 1526, 1703, 1939, 3146
CseI	GACGC	3 cut(s)	1194, 2061, 2765
CsiI	ACCWGGT	1 cut(s)	1954
CspCI	CAANNNNNGTGG	2 cut(s)	2121, 2156
DraI	TTTAAA	2 cut(s)	2203, 2271
EaeI	YGGCCR	1 cut(s)	226
Eam1104I	CTCTTC	4 cut(s)	2601, 2862, 3074, 3357
EarI	CTCTTC	4 cut(s)	2601, 2862, 3074, 3357
EciI	GGCGGA	1 cut(s)	41
Eco130I	CCWWGG	4 cut(s)	352, 1183, 1902, 2514
Eco31I	GGTCTC	1 cut(s)	2355
Eco32I	GATATC	1 cut(s)	3300
Eco47I	GGWCC	5 cut(s)	147, 196, 1526, 1939, 3146
Eco57I	CTGAAG	8 cut(s)	426, 443, 575, 1158, 1735, 2094, 2205, 3408
Eco88I	CYCGRG	1 cut(s)	2883
EcoO109I	RGGNCCY	2 cut(s)	1526, 3146
EcoRI	GAATTC	1 cut(s)	1350
EcoRII	CCWGG	4 cut(s)	1630, 1954, 2017, 2227
EcoRV	GATATC	1 cut(s)	3300
EcoT14I	CCWWGG	4 cut(s)	352, 1183, 1902, 2514
EcoT22I	ATGCAT	1 cut(s)	2285
ErhI	CCWWGG	4 cut(s)	352, 1183, 1902, 2514
Esp3I	CGTCTC	1 cut(s)	101
FalI	AAGNNNNNCTT	4 cut(s)	1350, 1382, 2859, 2891
FaqI	GGGAC	5 cut(s)	133, 1043, 1192, 1964, 3233
FauI	CCCGC	4 cut(s)	15, 158, 190, 539
FblI	GTMKAC	2 cut(s)	2981, 3172
GlaI	GCGC	4 cut(s)	89, 91, 154, 2728
GsaI	CCCAGC	1 cut(s)	3455
GsuI	CTGGAG	3 cut(s)	1251, 1625, 2146
HaeIII	GGCC	4 cut(s)	32, 228, 1704, 2881
HapII	CCGG	6 cut(s)	16, 145, 216, 1812, 2687, 3368
HgaI	GACGC	3 cut(s)	1194, 2061, 2765
HhaI	GCGC	4 cut(s)	90, 92, 155, 2729
Hin6I	GCGC	4 cut(s)	88, 90, 153, 2727
HinP1I	GCGC	4 cut(s)	88, 90, 153, 2727
HincII	GTYRAC	4 cut(s)	840, 1068, 1245, 2158
HindII	GTYRAC	4 cut(s)	840, 1068, 1245, 2158
HindIII	AAGCTT	5 cut(s)	609, 1883, 2196, 2340, 2546
HpaI	GTTAAC	1 cut(s)	2158
HpaII	CCGG	6 cut(s)	16, 145, 216, 1812, 2687, 3368
HphI	GGTGA	1 cut(s)	2118
Hpy99I	CGWCG	4 cut(s)	389, 392, 2055, 3012
HpyCH4III	ACNGT	5 cut(s)	1773, 2060, 2155, 2638, 3418
HpyCH4IV	ACGT	2 cut(s)	2032, 2826
HpySE526I	ACGT	2 cut(s)	2032, 2826
HspAI	GCGC	4 cut(s)	88, 90, 153, 2727
KspAI	GTTAAC	1 cut(s)	2158
LguI	GCTCTTC	2 cut(s)	2601, 3074
LmnI	GCTCC	4 cut(s)	12, 280, 2658, 2724
LweI	GCATC	6 cut(s)	358, 1021, 1081, 1157, 2400, 3400
MabI	ACCWGGT	1 cut(s)	1954
MaeII	ACGT	2 cut(s)	2032, 2826
MaeIII	GTNAC	5 cut(s)	870, 1192, 2060, 2248, 3434
MbiI	CCGCTC	1 cut(s)	546
MfeI	CAATTG	1 cut(s)	639
MflI	RGATCY	4 cut(s)	1431, 1657, 1831, 2224
MhlI	GDGCHC	6 cut(s)	457, 665, 754, 934, 2791, 3086
MlyI	GAGTC	6 cut(s)	610, 662, 984, 2707, 3341, 3392
MmeI	TCCRAC	2 cut(s)	397, 2224
Mph1103I	ATGCAT	1 cut(s)	2285
MroXI	GAANNNNTTC	4 cut(s)	1384, 1489, 1588, 2871
MslI	CAYNNNNRTG	1 cut(s)	807
MspA1I	CMGCKG	2 cut(s)	622, 1037
MspI	CCGG	6 cut(s)	16, 145, 216, 1812, 2687, 3368
MspR9I	CCNGG	5 cut(s)	145, 1632, 1956, 2019, 2229
MunI	CAATTG	1 cut(s)	639
Mva1269I	GAATGC	1 cut(s)	2299
MvaI	CCWGG	4 cut(s)	1632, 1956, 2019, 2229
MvnI	CGCG	4 cut(s)	90, 153, 223, 3022
NciI	CCSGG	1 cut(s)	145
NcoI	CCATGG	1 cut(s)	1183
NheI	GCTAGC	2 cut(s)	1550, 2413
NlaIV	GGNNCC	8 cut(s)	14, 149, 198, 350, 1306, 1486, 1848, 3066
NmuCI	GTSAC	2 cut(s)	870, 3434
NsiI	ATGCAT	1 cut(s)	2285
NspI	RCATGY	4 cut(s)	1112, 1841, 2190, 3367
NspV	TTCGAA	1 cut(s)	2170
PaeI	GCATGC	1 cut(s)	3367
PaeR7I	CTCGAG	1 cut(s)	2883
PagI	TCATGA	3 cut(s)	712, 1087, 2551
PaqCI	CACCTGC	2 cut(s)	362, 2288
PauI	GCGCGC	1 cut(s)	88
PciSI	GCTCTTC	2 cut(s)	2601, 3074
PcsI	WCGNNNNNNNCGW	4 cut(s)	73, 209, 2038, 2890
PctI	GAATGC	1 cut(s)	2299
PdmI	GAANNNNTTC	4 cut(s)	1384, 1489, 1588, 2871
PfeI	GAWTC	8 cut(s)	209, 677, 2101, 2146, 2275, 2900, 2939, 3497
PflFI	GACNNNGTC	1 cut(s)	145
PflMI	CCANNNNNTGG	2 cut(s)	985, 3458
PfoI	TCCNGGA	1 cut(s)	2017
PleI	GAGTC	6 cut(s)	610, 661, 983, 2706, 3341, 3392
PpsI	GAGTC	6 cut(s)	610, 661, 983, 2706, 3341, 3392
PpuMI	RGGWCCY	2 cut(s)	1526, 3146
PshBI	ATTAAT	2 cut(s)	1742, 2610
Psp1406I	AACGTT	1 cut(s)	2826
Psp5II	RGGWCCY	2 cut(s)	1526, 3146
Psp6I	CCWGG	4 cut(s)	1630, 1954, 2017, 2227
PspFI	CCCAGC	1 cut(s)	3451
PspGI	CCWGG	4 cut(s)	1630, 1954, 2017, 2227
PspN4I	GGNNCC	8 cut(s)	14, 149, 198, 350, 1306, 1486, 1848, 3066
PspPI	GGNCC	6 cut(s)	147, 196, 1526, 1703, 1939, 3146
PspPPI	RGGWCCY	2 cut(s)	1526, 3146
PspXI	VCTCGAGB	1 cut(s)	2883
PsrI	GAACNNNNNNTAC	2 cut(s)	2938, 2970
PstI	CTGCAG	1 cut(s)	1083
PstNI	CAGNNNCTG	7 cut(s)	874, 1376, 1976, 2228, 2963, 3458, 3500
PsuI	RGATCY	4 cut(s)	1431, 1657, 1831, 2224
PsyI	GACNNNGTC	1 cut(s)	145
PteI	GCGCGC	1 cut(s)	88
PvuII	CAGCTG	2 cut(s)	622, 1037
RseI	CAYNNNNRTG	1 cut(s)	807
SapI	GCTCTTC	2 cut(s)	2601, 3074
Sau96I	GGNCC	6 cut(s)	147, 196, 1526, 1703, 1939, 3146
ScaI	AGTACT	1 cut(s)	2213
SchI	GAGTC	6 cut(s)	610, 662, 984, 2707, 3341, 3392
ScrFI	CCNGG	5 cut(s)	145, 1632, 1956, 2019, 2229
SduI	GDGCHC	6 cut(s)	457, 665, 754, 934, 2791, 3086
SexAI	ACCWGGT	1 cut(s)	1954
SfaNI	GCATC	6 cut(s)	358, 1021, 1081, 1157, 2400, 3400
SfcI	CTRYAG	5 cut(s)	1079, 2862, 2955, 3417, 3480
Sfr274I	CTCGAG	1 cut(s)	2883
SfuI	TTCGAA	1 cut(s)	2170
SinI	GGWCC	5 cut(s)	147, 196, 1526, 1939, 3146
SlaI	CTCGAG	1 cut(s)	2883
SmiMI	CAYNNNNRTG	1 cut(s)	807
SmlI	CTYRAG	3 cut(s)	884, 1197, 2883
SmoI	CTYRAG	3 cut(s)	884, 1197, 2883
SphI	GCATGC	1 cut(s)	3367
SspI	AATATT	2 cut(s)	1795, 2119
StyD4I	CCNGG	5 cut(s)	143, 1630, 1954, 2017, 2227
StyI	CCWWGG	4 cut(s)	352, 1183, 1902, 2514
TaaI	ACNGT	5 cut(s)	1773, 2060, 2155, 2638, 3418
TaiI	ACGT	2 cut(s)	2035, 2829
TaqI	TCGA	7 cut(s)	922, 1279, 2170, 2336, 2785, 2822, 2884
TatI	WGTACW	9 cut(s)	691, 849, 1253, 1363, 1746, 2211, 2385, 2887, 3376
TauI	GCSGC	5 cut(s)	188, 231, 234, 794, 1557
TfiI	GAWTC	8 cut(s)	209, 677, 2101, 2146, 2275, 2900, 2939, 3497
TscAI	CASTG	6 cut(s)	879, 1351, 2145, 2188, 2568, 2725
TseFI	GTSAC	2 cut(s)	870, 3434
Tsp45I	GTSAC	2 cut(s)	870, 3434
TspRI	CASTG	6 cut(s)	879, 1351, 2145, 2188, 2568, 2725
Tth111I	GACNNNGTC	1 cut(s)	145
Van91I	CCANNNNNTGG	2 cut(s)	985, 3458
VpaK11BI	GGWCC	5 cut(s)	147, 196, 1526, 1939, 3146
VspI	ATTAAT	2 cut(s)	1742, 2610
XapI	RAATTY	5 cut(s)	420, 1350, 1380, 1966, 2080
XceI	RCATGY	4 cut(s)	1112, 1841, 2190, 3367
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2170
XhoI	CTCGAG	1 cut(s)	2883
XmaJI	CCTAGG	1 cut(s)	352
XmiI	GTMKAC	2 cut(s)	2981, 3172
XmnI	GAANNNNTTC	4 cut(s)	1384, 1489, 1588, 2871
ZrmI	AGTACT	1 cut(s)	2213
Zsp2I	ATGCAT	1 cut(s)	2285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.