Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003360

Physical Location & Seq

Genomic Coordinates Forward (+)

192765 .. 194315

Gene Structure 1551 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G000110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGCCGTCCAGTTTCAATTTGTTTATGAAAGGACCTATTGTGAAAAAGTCATCTGGTAGCAGTTCTGGGAAGCCAGAAGATACCAGTGGAAGCGCTCCTGAGGTTTCAGCTCGTGAGAAATTATTGAACGAACAGCCTGGTCTTCTTCAGCAATTTGGAATGGATCTCCTTCCTGTGTTTATACAGATTTATGGCTCTAGTGTAAATGGCCCTGTTCGCCACAAATGTCTATCGGTCATCGGCAAGTTAATGTACTTCAGCCCTGCAGAGATGATTGAGTCTTTGTTAAGTATGACAAATATTGCAAGTTTCTTAGCCGGTGTTTTAGCATGGAAAGATCCGCATGTCTTGGTTCCTGCTCTCCAAATTGCCGAGATACTCATGGAGAAGCTTCCCAAGACTTTCTCCAAGGTGTTTGTCAGAGAAGGTGTGGTTCATGCTGTTGACCAACTTATTTTAGCTGGTACTCCGAATTCAGTTACTTCACAAGTCTCTTCTGCTGAAAAGGACAATGATTCTGTGCCGGGATCATCACGTTCTCGGCGTTATAGACGTCGCAACAGTAACTCAAATCCAGATGGAAATTCATTGGAAGAATCGAAGATTCCTGCTTCGGTAACTGTTGGTTCGCCTCCAAGTTCAGTGGAGATCCCAACTGTCAACTCCAGCCTTCGTGTAGCTGTGAGTACATGTGCAAAAGCGTTTAAGGACAAGTACTTCCCTTCAGACCCTGGGGCGGGTGAAGTTGGTGTCACCGATGATCTATTACATTTAAAAAATCTGTGTGTGAAGTTGAATGCCGGTGTAGATGACCCAAAAACAAAAGCAAAGGGAAAATCAAAAGCTTCTGGGTCTCGCTTAGTTGATAGTTATGCATATAAGGAAGAGTATTTGATTGGTGTGGTAGCCGAGATGGTAGCCGAACTAAGCAAAGGGGATGGTATGTCTACCTTTGAGTTCATAGGTAGCGGTGTTGTCGCAGCTTTGCTGAACTATTTTTCTTGTGGCTACTTCTCGAAGGAGAGAATTTCAGAAGCTAATTTGCCTAAGCTTCGTCAACAAGCCCTGAAGTGGTTTAAGTCATTTGTTGCTGTTGCTCTTCCTTTGAGTATTGATGAAGGAAGAGTAGCCCCAATGACTATCACAGTTCAGAAGCTACAAGGTGCTTTGTCCTCTTTAGAGCGGTTCCCTGTTGTGCTGAGTCATTCATCTAGGTCATGTACTGGAAATGCACGCCTCTCCTCTGGACTTAGTGCATTATCCCAGCCCTTGAAGTTGCGCCTATGTCGAGCACAAGGAGAAAAGGCTCTCCGAGATTATTCATCGAATGCTGTACTGATTGACCCATTAGCAAGCTTAGCTGCTGTTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006464 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0030154 GO:0032446 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0036211 GO:0042023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:0140096 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

457

Amino Acids

48.97

Weight (kDa)

9.1

Isoelectric Point (pI)

44.91

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38600 AT4G38600 AT4G38600
fragaria_vesca	FvH4_2g01290
malus_domestica	MD00G1173700.v1.1 MD00G1173800.v1.1 MD00G1173900.v1.1 MD05G1120900.v1.1 MD10G1095300.v1.1
prunus_persica	Prupe.8G164700_v2.0.a1
pyrus_communis	pycom05g11490 pycom05g11500 pycom10g10610 pycom10g10620
rosa_chinensis	RchiOBHm_Chr6g0251951 RchiOBHm_Chr6g0252001
rosa_laevigata	RLG00000015410 RLG00000015417
rosa_multiflora	Rmu_sc0002522.1_g000052 Rmu_sc0002522.1_g000062 Rmu_ssc0000303.1_g000016
rosa_roxburghii	Rroxscaffold_7G00216270
rosa_rugosa	Rorug05G0505300.1
rosa_samantha	Rh6AG016800 Rh6AG017300 Rh6AG017400 Rh6AG018000 Rh6AG018100 Rh6AG018900 Rh6AG019000 Rh6CG011400 Rh6DG012700
rosa_wichuraiana	Rw6G000110 Rw6G000120 Rw6G000150 Rw6G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	556
AccBSI	CCGCTC	1 cut(s)	1183
AccI	GTMKAC	1 cut(s)	947
AciI	CCGC	4 cut(s)	341, 737, 969, 1183
AclWI	GGATC	4 cut(s)	171, 332, 535, 643
AcsI	RAATTY	3 cut(s)	472, 583, 1026
AcuI	CTGAAG	4 cut(s)	131, 241, 708, 1088
AcyI	GRCGYC	1 cut(s)	553
AfaI	GTAC	6 cut(s)	254, 466, 688, 716, 1222, 1335
AfeI	AGCGCT	1 cut(s)	94
AfiI	CCNNNNNNNGG	3 cut(s)	736, 737, 1071
AflIII	ACRYGT	1 cut(s)	689
AgsI	TTSAA	5 cut(s)	16, 127, 796, 1273, 1370
AjnI	CCWGG	2 cut(s)	136, 730
Alw21I	GWGCWC	1 cut(s)	1294
Alw26I	GTCTC	2 cut(s)	496, 858
AlwI	GGATC	4 cut(s)	171, 332, 535, 643
AlwNI	CAGNNNCTG	1 cut(s)	731
Aor51HI	AGCGCT	1 cut(s)	94
AoxI	GGCC	1 cut(s)	208
ApeKI	GCWGC	2 cut(s)	980, 1361
ApoI	RAATTY	3 cut(s)	472, 583, 1026
AspLEI	GCGC	2 cut(s)	95, 1281
AspS9I	GGNCC	2 cut(s)	32, 209
AsuC2I	CCSGG	1 cut(s)	525
AsuHPI	GGTGA	2 cut(s)	745, 752
AvaII	GGWCC	1 cut(s)	32
AxyI	CCTNAGG	1 cut(s)	99
BauI	CACGAG	1 cut(s)	111
BbsI	GAAGAC	1 cut(s)	134
Bbv12I	GWGCWC	1 cut(s)	1294
BbvI	GCAGC	2 cut(s)	992, 1348
BccI	CCATC	3 cut(s)	572, 907, 932
BciT130I	CCWGG	2 cut(s)	138, 732
BcnI	CCSGG	1 cut(s)	525
BcoDI	GTCTC	2 cut(s)	496, 858
BfaI	CTAG	2 cut(s)	198, 1212
BfmI	CTRYAG	1 cut(s)	264
BfoI	RGCGCY	1 cut(s)	96
BisI	GCNGC	2 cut(s)	981, 1362
BlpI	GCTNAGC	1 cut(s)	1357
BlsI	GCNGC	2 cut(s)	982, 1363
BmcAI	AGTACT	1 cut(s)	716
Bme1390I	CCNGG	3 cut(s)	138, 525, 732
Bme18I	GGWCC	1 cut(s)	32
BmgT120I	GGNCC	2 cut(s)	32, 209
BmiI	GGNNCC	2 cut(s)	354, 1187
BmrFI	CCNGG	3 cut(s)	138, 525, 732
BpiI	GAAGAC	1 cut(s)	134
BpmI	CTGGAG	1 cut(s)	649
Bpu10I	CCTNAGC	1 cut(s)	1047
Bpu1102I	GCTNAGC	1 cut(s)	1357
BpuMI	CCSGG	1 cut(s)	525
BsaHI	GRCGYC	1 cut(s)	553
BsaI	GGTCTC	1 cut(s)	858
BsaJI	CCNNGG	3 cut(s)	408, 730, 731
Bsc4I	CCNNNNNNNGG	3 cut(s)	736, 737, 1071
Bse118I	RCCGGY	2 cut(s)	317, 800
Bse1I	ACTGG	3 cut(s)	9, 84, 1228
Bse21I	CCTNAGG	1 cut(s)	99
BseBI	CCWGG	2 cut(s)	138, 732
BseDI	CCNNGG	3 cut(s)	408, 730, 731
BseGI	GGATG	1 cut(s)	943
BseLI	CCNNNNNNNGG	3 cut(s)	736, 737, 1071
BseMII	CTCAG	2 cut(s)	90, 1190
BseNI	ACTGG	3 cut(s)	9, 84, 1228
BseRI	GAGGAG	1 cut(s)	1231
BseXI	GCAGC	2 cut(s)	992, 1348
BseYI	CCCAGC	1 cut(s)	1263
BshFI	GGCC	1 cut(s)	210
BsiHKAI	GWGCWC	1 cut(s)	1294
BsiSI	CCGG	3 cut(s)	318, 524, 801
BslI	CCNNNNNNNGG	3 cut(s)	736, 737, 1071
BsmAI	GTCTC	2 cut(s)	496, 858
BsmI	GAATGC	2 cut(s)	802, 1333
BsnI	GGCC	1 cut(s)	210
Bso31I	GGTCTC	1 cut(s)	858
Bsp1286I	GDGCHC	1 cut(s)	1294
Bsp143I	GATC	5 cut(s)	163, 337, 527, 648, 760
Bsp1720I	GCTNAGC	1 cut(s)	1357
BspACI	CCGC	4 cut(s)	341, 737, 969, 1183
BspANI	GGCC	1 cut(s)	210
BspCNI	CTCAG	2 cut(s)	91, 1191
BspLI	GGNNCC	2 cut(s)	354, 1187
BspMAI	CTGCAG	1 cut(s)	268
BspPI	GGATC	4 cut(s)	171, 332, 535, 643
BspQI	GCTCTTC	1 cut(s)	1104
BspTNI	GGTCTC	1 cut(s)	858
BsrBI	CCGCTC	1 cut(s)	1183
BsrFI	RCCGGY	2 cut(s)	317, 800
BsrI	ACTGG	3 cut(s)	9, 84, 1228
BssAI	RCCGGY	2 cut(s)	317, 800
BssECI	CCNNGG	3 cut(s)	408, 730, 731
BssMI	GATC	5 cut(s)	163, 337, 527, 648, 760
BssNI	GRCGYC	1 cut(s)	553
BssSI	CACGAG	1 cut(s)	111
BssT1I	CCWWGG	1 cut(s)	408
Bst2BI	CACGAG	1 cut(s)	111
Bst2UI	CCWGG	2 cut(s)	138, 732
Bst4CI	ACNGT	4 cut(s)	563, 622, 658, 1147
Bst6I	CTCTTC	4 cut(s)	499, 879, 1104, 1117
BstACI	GRCGYC	1 cut(s)	553
BstC8I	GCNNGC	2 cut(s)	1234, 1354
BstDEI	CTNAG	8 cut(s)	99, 313, 859, 926, 1047, 1199, 1250, 1357
BstF5I	GGATG	1 cut(s)	943
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	2 cut(s)	95, 1281
BstKTI	GATC	5 cut(s)	166, 340, 530, 651, 763
BstMAI	GTCTC	2 cut(s)	496, 858
BstMBI	GATC	5 cut(s)	163, 337, 527, 648, 760
BstMWI	GCNNNNNNNGC	2 cut(s)	216, 1358
BstNI	CCWGG	2 cut(s)	138, 732
BstNSI	RCATGY	2 cut(s)	347, 693
BstSCI	CCNGG	3 cut(s)	136, 523, 730
BstSFI	CTRYAG	1 cut(s)	264
BstV1I	GCAGC	2 cut(s)	992, 1348
BstV2I	GAAGAC	1 cut(s)	134
BstX2I	RGATCY	3 cut(s)	163, 337, 648
BstYI	RGATCY	3 cut(s)	163, 337, 648
Bsu36I	CCTNAGG	1 cut(s)	99
BsuRI	GGCC	1 cut(s)	210
BtsCI	GGATG	1 cut(s)	943
BtsIMutI	CAGTG	2 cut(s)	91, 648
Cac8I	GCNNGC	2 cut(s)	1234, 1354
CaiI	CAGNNNCTG	1 cut(s)	731
CfoI	GCGC	2 cut(s)	95, 1281
Cfr10I	RCCGGY	2 cut(s)	317, 800
Cfr13I	GGNCC	2 cut(s)	32, 209
Csp6I	GTAC	6 cut(s)	253, 465, 687, 715, 1221, 1334
CspCI	CAANNNNNGTGG	2 cut(s)	624, 659
CviAII	CATG	6 cut(s)	330, 344, 382, 437, 690, 1218
CviQI	GTAC	6 cut(s)	253, 465, 687, 715, 1221, 1334
DdeI	CTNAG	8 cut(s)	99, 313, 859, 926, 1047, 1199, 1250, 1357
DpnI	GATC	5 cut(s)	165, 339, 529, 650, 762
DpnII	GATC	5 cut(s)	163, 337, 527, 648, 760
DraI	TTTAAA	1 cut(s)	774
Eam1104I	CTCTTC	4 cut(s)	499, 879, 1104, 1117
EarI	CTCTTC	4 cut(s)	499, 879, 1104, 1117
Eco130I	CCWWGG	1 cut(s)	408
Eco31I	GGTCTC	1 cut(s)	858
Eco47I	GGWCC	1 cut(s)	32
Eco47III	AGCGCT	1 cut(s)	94
Eco57I	CTGAAG	4 cut(s)	131, 241, 708, 1088
Eco81I	CCTNAGG	1 cut(s)	99
EcoO109I	RGGNCCY	1 cut(s)	32
EcoRI	GAATTC	1 cut(s)	472
EcoRII	CCWGG	2 cut(s)	136, 730
EcoT14I	CCWWGG	1 cut(s)	408
EcoT22I	ATGCAT	1 cut(s)	877
ErhI	CCWWGG	1 cut(s)	408
FaeI	CATG	6 cut(s)	333, 347, 385, 440, 693, 1221
FatI	CATG	6 cut(s)	329, 343, 381, 436, 689, 1217
FauI	CCCGC	1 cut(s)	730
FblI	GTMKAC	1 cut(s)	947
Fnu4HI	GCNGC	2 cut(s)	981, 1362
FokI	GGATG	1 cut(s)	950
Fsp4HI	GCNGC	2 cut(s)	981, 1362
FspBI	CTAG	2 cut(s)	198, 1212
GlaI	GCGC	2 cut(s)	94, 1280
GluI	GCNGC	2 cut(s)	981, 1362
GsaI	CCCAGC	1 cut(s)	1267
GsuI	CTGGAG	1 cut(s)	649
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	1 cut(s)	210
HapII	CCGG	3 cut(s)	318, 524, 801
HhaI	GCGC	2 cut(s)	95, 1281
Hin1I	GRCGYC	1 cut(s)	553
Hin1II	CATG	6 cut(s)	333, 347, 385, 440, 693, 1221
Hin6I	GCGC	2 cut(s)	93, 1279
HinP1I	GCGC	2 cut(s)	93, 1279
HincII	GTYRAC	3 cut(s)	445, 661, 1058
HindII	GTYRAC	3 cut(s)	445, 661, 1058
HindIII	AAGCTT	4 cut(s)	389, 843, 1049, 1354
HinfI	GANTC	5 cut(s)	278, 515, 596, 604, 1201
HpaII	CCGG	3 cut(s)	318, 524, 801
HphI	GGTGA	2 cut(s)	745, 752
Hpy166II	GTNNAC	4 cut(s)	445, 661, 948, 1058
Hpy188I	TCNGA	6 cut(s)	422, 471, 727, 1033, 1152, 1313
Hpy188III	TCNNGA	5 cut(s)	98, 113, 575, 1015, 1245
Hpy8I	GTNNAC	4 cut(s)	445, 661, 948, 1058
Hpy99I	CGWCG	1 cut(s)	558
HpyAV	CCTTC	6 cut(s)	179, 419, 680, 732, 1012, 1112
HpyCH4III	ACNGT	4 cut(s)	563, 622, 658, 1147
HpyCH4IV	ACGT	2 cut(s)	535, 553
HpyCH4V	TGCA	6 cut(s)	266, 305, 695, 875, 1232, 1256
HpyF10VI	GCNNNNNNNGC	2 cut(s)	216, 1358
HpyF3I	CTNAG	8 cut(s)	99, 313, 859, 926, 1047, 1199, 1250, 1357
HpySE526I	ACGT	2 cut(s)	535, 553
Hsp92I	GRCGYC	1 cut(s)	553
Hsp92II	CATG	6 cut(s)	333, 347, 385, 440, 693, 1221
HspAI	GCGC	2 cut(s)	93, 1279
Kzo9I	GATC	5 cut(s)	163, 337, 527, 648, 760
LguI	GCTCTTC	1 cut(s)	1104
LmnI	GCTCC	1 cut(s)	100
Lsp1109I	GCAGC	2 cut(s)	992, 1348
MaeI	CTAG	2 cut(s)	198, 1212
MaeII	ACGT	2 cut(s)	535, 553
MaeIII	GTNAC	4 cut(s)	478, 563, 616, 751
MalI	GATC	5 cut(s)	165, 339, 529, 650, 762
MbiI	CCGCTC	1 cut(s)	1183
MboI	GATC	5 cut(s)	163, 337, 527, 648, 760
MboII	GAAGA	9 cut(s)	89, 134, 137, 486, 605, 613, 896, 1091, 1134
MflI	RGATCY	3 cut(s)	163, 337, 648
MhlI	GDGCHC	1 cut(s)	1294
MluCI	AATT	8 cut(s)	16, 119, 152, 366, 472, 583, 1026, 1039
MlyI	GAGTC	2 cut(s)	287, 1210
MnlI	CCTC	5 cut(s)	94, 642, 1183, 1247, 1252
Mph1103I	ATGCAT	1 cut(s)	877
MseI	TTAA	5 cut(s)	248, 287, 705, 773, 1077
MspI	CCGG	3 cut(s)	318, 524, 801
MspR9I	CCNGG	3 cut(s)	138, 525, 732
Mva1269I	GAATGC	2 cut(s)	802, 1333
MvaI	CCWGG	2 cut(s)	138, 732
MwoI	GCNNNNNNNGC	2 cut(s)	216, 1358
NciI	CCSGG	1 cut(s)	525
NdeII	GATC	5 cut(s)	163, 337, 527, 648, 760
NlaIII	CATG	6 cut(s)	333, 347, 385, 440, 693, 1221
NlaIV	GGNNCC	2 cut(s)	354, 1187
NmeAIII	GCCGAG	3 cut(s)	397, 520, 934
NmuCI	GTSAC	1 cut(s)	751
NsiI	ATGCAT	1 cut(s)	877
NspI	RCATGY	2 cut(s)	347, 693
PasI	CCCWGGG	1 cut(s)	731
PciI	ACATGT	1 cut(s)	689
PciSI	GCTCTTC	1 cut(s)	1104
PcsI	WCGNNNNNNNCGW	1 cut(s)	541
PctI	GAATGC	2 cut(s)	802, 1333
PfeI	GAWTC	3 cut(s)	515, 596, 604
PkrI	GCNGC	2 cut(s)	982, 1363
PleI	GAGTC	2 cut(s)	286, 1209
PpsI	GAGTC	2 cut(s)	286, 1209
PpuMI	RGGWCCY	1 cut(s)	32
PscI	ACATGT	1 cut(s)	689
Psp5II	RGGWCCY	1 cut(s)	32
Psp6I	CCWGG	2 cut(s)	136, 730
PspFI	CCCAGC	1 cut(s)	1263
PspGI	CCWGG	2 cut(s)	136, 730
PspN4I	GGNNCC	2 cut(s)	354, 1187
PspPI	GGNCC	2 cut(s)	32, 209
PspPPI	RGGWCCY	1 cut(s)	32
PstI	CTGCAG	1 cut(s)	268
PstNI	CAGNNNCTG	1 cut(s)	731
PsuI	RGATCY	3 cut(s)	163, 337, 648
RsaI	GTAC	6 cut(s)	254, 466, 688, 716, 1222, 1335
RsaNI	GTAC	6 cut(s)	253, 465, 687, 715, 1221, 1334
SapI	GCTCTTC	1 cut(s)	1104
SaqAI	TTAA	5 cut(s)	248, 287, 705, 773, 1077
SatI	GCNGC	2 cut(s)	981, 1362
Sau3AI	GATC	5 cut(s)	163, 337, 527, 648, 760
Sau96I	GGNCC	2 cut(s)	32, 209
ScaI	AGTACT	1 cut(s)	716
SchI	GAGTC	2 cut(s)	287, 1210
ScrFI	CCNGG	3 cut(s)	138, 525, 732
SduI	GDGCHC	1 cut(s)	1294
SfcI	CTRYAG	1 cut(s)	264
SinI	GGWCC	1 cut(s)	32
Sse9I	AATT	8 cut(s)	16, 119, 152, 366, 472, 583, 1026, 1039
SsiI	CCGC	4 cut(s)	341, 737, 969, 1183
SspI	AATATT	1 cut(s)	301
SspMI	CTAG	2 cut(s)	198, 1212
StyD4I	CCNGG	3 cut(s)	136, 523, 730
StyI	CCWWGG	1 cut(s)	408
TaaI	ACNGT	4 cut(s)	563, 622, 658, 1147
TaiI	ACGT	2 cut(s)	538, 556
TaqI	TCGA	4 cut(s)	599, 1016, 1288, 1325
TaqII	GACCGA	1 cut(s)	223
TasI	AATT	8 cut(s)	16, 119, 152, 366, 472, 583, 1026, 1039
TatI	WGTACW	5 cut(s)	252, 686, 714, 1220, 1333
TfiI	GAWTC	3 cut(s)	515, 596, 604
Tru1I	TTAA	5 cut(s)	248, 287, 705, 773, 1077
Tru9I	TTAA	5 cut(s)	248, 287, 705, 773, 1077
TscAI	CASTG	2 cut(s)	91, 648
TseFI	GTSAC	1 cut(s)	751
TseI	GCWGC	2 cut(s)	980, 1361
Tsp45I	GTSAC	1 cut(s)	751
TspDTI	ATGAA	7 cut(s)	41, 425, 576, 949, 1131, 1197, 1311
TspRI	CASTG	2 cut(s)	91, 648
VpaK11BI	GGWCC	1 cut(s)	32
XapI	RAATTY	3 cut(s)	472, 583, 1026
XceI	RCATGY	2 cut(s)	347, 693
XmiI	GTMKAC	1 cut(s)	947
XspI	CTAG	2 cut(s)	198, 1212
ZraI	GACGTC	1 cut(s)	554
ZrmI	AGTACT	1 cut(s)	716
Zsp2I	ATGCAT	1 cut(s)	877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.