Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0003360

Physical Location & Seq

Genomic Coordinates Forward (+)

14026280 .. 14029428

Gene Structure 3149 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g10610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGAAACTCGGAGCCGGAAGCGGGCGGAGGCCACCTCAGCAGCCCCTTCTTCTTCTTCGTCTGGTCGCACCACTCGTTCTTCCAAGCGCGCTCGTCTCTCTGCTTCTTCATTATCTTCTGTTAGCCCCACTCCGATTACGACCCGGTCCCGCGCCTCTCGCACCCAAAACGAACCCGCACCCGCCGCACCCATGGACCCCACGAACGAATCGTCCGGCTCACGCGGCCGCCGGTTGAATATGGATGCAGGTGGAACTGGCGACGACGATGACAATGATAGCGAAGGCGGTGTTGGGATTTTGCACCAGAACTTGACTTCAGCGAGTAGTGCCCTTCAGGGGCTTCTTAGGAAGATCGGTGCCGGACTGGATGACCTGCTTCCTTCTTCGGCTATGGGTTCGGCTTCGTCCTCGCACCAGAGCGGGAGGCTGAAGAAGATTTTATCTGGGTTAAGAGCTGATGGGGAAGAAGGGAAACAGGTGGAAGCTTTGACTCAACTGTGTGAAATGCTTTCAATTGGAACTGAGGAGTCGTTGAGCACGTTTTCGGTTGATTCATTTGTGCCTGTGCTTGTGGGATTGCTTAATCATGAGAGCAATCCAGATATTATGCTTCTTGCAGCTAGAGCACTCACTCATCTTTGCGATGTGCTTCCATCGTCTTGTGCCGCCGTGGTACATTATGGTGCTGTTTCTTGCTTTTGTGCAAGGTTGTTGACGATTGAGTACATGGACTTGGCAGAGCAGTCACTGCAAGCTCTCAAGAAAATATCACAAGAACACCCAACTGCCTGTCTTCGAGCCGGTGCTCTTATGGCAGTGCTTTCTTATCTGGATTTTTTCTCCACAGGAGTCCAGCGTGTGGCATTATCAACTGCTGCGAATATGTGCAAGAAACTTCCTTTAGATGCAGCTGACTTTGTGATGGAAGCTGTCCCATTGTTGACAAACCTTCTGCAGTATCATGATGCAAAGGTTTTGGAACATGCTTCTGTATGTCTGACACGAATTGCTGAAGCATTTGCATCATCTCCTGACAAACTTGATGAATTATGCAACCATGGGCTAGTTACTCACGCTGCGTCCCTTATTTCCACCAGCAATTCTGGAGGTGGTCAGTCAACTCTAAGTACACCTACTTATACTGGTTTAATTCGACTACTATCAACCTGTGCAAGTGGTTCCCCATTAGGGTCTAAAACTTTGCTTCTTCTTGGGATCGATGGAATTCTTAAAGATGTACTTTCAGGTTCTGGAATTTCTTGTAGCACATCAGTTTCTCCTGCTTTAAGTAAGCCACCAGAGCAGATCTTTGAGATTGTCAATCTGGCAAATGAGCTACTTCCTCCATTGCCACAAGGAACCATTTCCATTCCATCCAGCTTCAATGTATTTATGAAGGGTCCTGTTGTGAAAAAGTCGTCTGCTAGCGGCTCTGGGAAACAAGAAGACACCAATGGAACTGGTTCTGAGGTTTCAGCTCGTGAGAAATTATTGAATGAACAGCCTGGGCTTCTCCAGCAATTTGGAATGATCGATCTCCTTCCTGTTTTAATACAGATTTATGGTTCTAGCGTTAATGGCCCTGTTCGCCACAAATGTCTTTCAGTAATTGGGAAATTAATGTACTTCAGCAATGCAGAGATGATACAGTCTTTATTAAGTTCAACAAATATTTCAAGTTTCTTAGCCGGTGTCTTAGCATGGAAAGATCCGCATGTCTTGGTTCCTGCTCTCCAAATTGCTGAGATACTCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006464 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0030154 GO:0032446 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0036211 GO:0042023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:0140096 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

587

Amino Acids

61.5

Weight (kDa)

6.09

Isoelectric Point (pI)

49.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	135 - 267	4.2e-51	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38600 AT4G38600 AT4G38600
fragaria_vesca	FvH4_2g01290
malus_domestica	MD00G1173700.v1.1 MD00G1173800.v1.1 MD00G1173900.v1.1 MD05G1120900.v1.1 MD10G1095300.v1.1
prunus_persica	Prupe.8G164700_v2.0.a1
pyrus_communis	pycom05g11490 pycom05g11500 pycom10g10610 pycom10g10620
rosa_chinensis	RchiOBHm_Chr6g0251951 RchiOBHm_Chr6g0252001
rosa_laevigata	RLG00000015410 RLG00000015417
rosa_multiflora	Rmu_sc0002522.1_g000052 Rmu_sc0002522.1_g000062 Rmu_ssc0000303.1_g000016
rosa_roxburghii	Rroxscaffold_7G00216270
rosa_rugosa	Rorug05G0505300.1
rosa_samantha	Rh6AG016800 Rh6AG017300 Rh6AG017400 Rh6AG018000 Rh6AG018100 Rh6AG018900 Rh6AG019000 Rh6CG011400 Rh6DG012700
rosa_wichuraiana	Rw6G000110 Rw6G000120 Rw6G000150 Rw6G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	239
Acc36I	ACCTGC	2 cut(s)	239, 384
AccB1I	GGYRCC	1 cut(s)	359
AccB7I	CCANNNNNTGG	1 cut(s)	862
AccBSI	CCGCTC	1 cut(s)	423
AccII	CGCG	3 cut(s)	90, 153, 225
AclWI	GGATC	2 cut(s)	1226, 1706
AcoI	YGGCCR	1 cut(s)	226
AcsI	RAATTY	2 cut(s)	1227, 1257
AcuI	CTGAAG	5 cut(s)	303, 320, 452, 1035, 1615
AfaI	GTAC	5 cut(s)	678, 728, 1132, 1242, 1628
AfiI	CCNNNNNNNGG	4 cut(s)	22, 339, 862, 1191
AgsI	TTSAA	6 cut(s)	238, 516, 1387, 1498, 1668, 1680
AjnI	CCWGG	1 cut(s)	1507
AjuI	GAANNNNNNNTTGG	2 cut(s)	276, 308
AloI	GAACNNNNNNTCC	2 cut(s)	197, 229
AluBI	AGCT	9 cut(s)	458, 488, 623, 758, 914, 932, 1339, 1383, 1481
AluI	AGCT	9 cut(s)	458, 488, 623, 758, 914, 932, 1339, 1383, 1481
Alw21I	GWGCWC	3 cut(s)	542, 631, 811
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	2 cut(s)	1226, 1706
AlwNI	CAGNNNCTG	2 cut(s)	751, 1253
AoxI	GGCC	3 cut(s)	30, 226, 1582
ApeKI	GCWGC	5 cut(s)	41, 620, 878, 911, 1079
ApoI	RAATTY	2 cut(s)	1227, 1257
ArsI	GACNNNNNNTTYG	2 cut(s)	197, 229
AseI	ATTAAT	1 cut(s)	1622
Asp700I	GAANNNNTTC	2 cut(s)	1366, 1465
AspLEI	GCGC	3 cut(s)	90, 92, 155
AspS9I	GGNCC	4 cut(s)	147, 196, 1403, 1583
AsuC2I	CCSGG	1 cut(s)	145
AsuNHI	GCTAGC	1 cut(s)	1427
AvaII	GGWCC	3 cut(s)	147, 196, 1403
BaeGI	GKGCMC	1 cut(s)	334
BaeI	ACNNNNGTAYC	1 cut(s)	1742
BanI	GGYRCC	1 cut(s)	359
BauI	CACGAG	1 cut(s)	1482
BbsI	GAAGAC	2 cut(s)	788, 1455
Bbv12I	GWGCWC	3 cut(s)	542, 631, 811
BbvCI	CCTCAGC	1 cut(s)	37
BbvI	GCAGC	5 cut(s)	53, 632, 865, 923, 1066
BccI	CCATC	5 cut(s)	455, 664, 919, 1217, 1384
BceAI	ACGGC	1 cut(s)	656
BciT130I	CCWGG	1 cut(s)	1509
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	1 cut(s)	101
BfaI	CTAG	4 cut(s)	624, 1067, 1428, 1572
BfmI	CTRYAG	1 cut(s)	956
BfuAI	ACCTGC	2 cut(s)	239, 384
BglII	AGATCT	1 cut(s)	1308
Bme1390I	CCNGG	2 cut(s)	145, 1509
Bme18I	GGWCC	3 cut(s)	147, 196, 1403
BmgT120I	GGNCC	4 cut(s)	147, 196, 1403, 1583
BmiI	GGNNCC	8 cut(s)	14, 149, 198, 361, 1183, 1363, 1404, 1728
BmrFI	CCNGG	2 cut(s)	145, 1509
BmsI	GCATC	4 cut(s)	235, 898, 958, 1034
BmtI	GCTAGC	1 cut(s)	1431
BpiI	GAAGAC	2 cut(s)	788, 1455
BplI	GAGNNNNNCTC	2 cut(s)	20, 52
BpmI	CTGGAG	2 cut(s)	1128, 1502
Bpu10I	CCTNAGC	1 cut(s)	37
BpuEI	CTTGAG	1 cut(s)	746
BpuMI	CCSGG	1 cut(s)	145
Bsa29I	ATCGAT	2 cut(s)	1221, 1536
BsaJI	CCNNGG	4 cut(s)	192, 672, 1060, 1508
Bsc4I	CCNNNNNNNGG	4 cut(s)	22, 339, 862, 1191
Bse118I	RCCGGY	3 cut(s)	231, 803, 1691
Bse1I	ACTGG	4 cut(s)	262, 372, 1150, 1468
Bse3DI	GCAATG	2 cut(s)	1349, 1642
BseBI	CCWGG	1 cut(s)	1509
BseCI	ATCGAT	2 cut(s)	1221, 1536
BseDI	CCNNGG	4 cut(s)	192, 672, 1060, 1508
BseGI	GGATG	3 cut(s)	250, 376, 1376
BseLI	CCNNNNNNNGG	4 cut(s)	22, 339, 862, 1191
BseMI	GCAATG	2 cut(s)	1349, 1642
BseMII	CTCAG	4 cut(s)	51, 516, 1461, 1737
BseNI	ACTGG	4 cut(s)	262, 372, 1150, 1468
BsePI	GCGCGC	1 cut(s)	88
BseRI	GAGGAG	1 cut(s)	542
BseSI	GKGCMC	1 cut(s)	334
BseX3I	CGGCCG	1 cut(s)	226
BseXI	GCAGC	5 cut(s)	53, 632, 865, 923, 1066
Bsh1236I	CGCG	3 cut(s)	90, 153, 225
Bsh1285I	CGRYCG	1 cut(s)	229
BshFI	GGCC	3 cut(s)	32, 228, 1584
BshNI	GGYRCC	1 cut(s)	359
BshVI	ATCGAT	2 cut(s)	1221, 1536
BsiEI	CGRYCG	1 cut(s)	229
BsiHKAI	GWGCWC	3 cut(s)	542, 631, 811
BsiSI	CCGG	7 cut(s)	16, 145, 216, 232, 363, 804, 1692
BslFI	GGGAC	3 cut(s)	133, 920, 1069
BslI	CCNNNNNNNGG	4 cut(s)	22, 339, 862, 1191
BsmAI	GTCTC	1 cut(s)	101
BsmBI	CGTCTC	1 cut(s)	101
BsmFI	GGGAC	3 cut(s)	133, 920, 1069
BsnI	GGCC	3 cut(s)	32, 228, 1584
Bsp1286I	GDGCHC	4 cut(s)	334, 542, 631, 811
Bsp143I	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
Bsp19I	CCATGG	2 cut(s)	192, 1060
BspANI	GGCC	3 cut(s)	32, 228, 1584
BspCNI	CTCAG	4 cut(s)	50, 517, 1462, 1738
BspDI	ATCGAT	2 cut(s)	1221, 1536
BspFNI	CGCG	3 cut(s)	90, 153, 225
BspHI	TCATGA	2 cut(s)	589, 964
BspLI	GGNNCC	8 cut(s)	14, 149, 198, 361, 1183, 1363, 1404, 1728
BspMAI	CTGCAG	1 cut(s)	960
BspMI	ACCTGC	2 cut(s)	239, 384
BspOI	GCTAGC	1 cut(s)	1431
BspPI	GGATC	2 cut(s)	1226, 1706
BspT107I	GGYRCC	1 cut(s)	359
BsrBI	CCGCTC	1 cut(s)	423
BsrDI	GCAATG	2 cut(s)	1349, 1642
BsrFI	RCCGGY	3 cut(s)	231, 803, 1691
BsrI	ACTGG	4 cut(s)	262, 372, 1150, 1468
BssAI	RCCGGY	3 cut(s)	231, 803, 1691
BssECI	CCNNGG	4 cut(s)	192, 672, 1060, 1508
BssHII	GCGCGC	1 cut(s)	88
BssMI	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
BssSI	CACGAG	1 cut(s)	1482
BssT1I	CCWWGG	2 cut(s)	192, 1060
Bst2BI	CACGAG	1 cut(s)	1482
Bst2UI	CCWGG	1 cut(s)	1509
Bst4CI	ACNGT	2 cut(s)	501, 1653
BstAPI	GCANNNNNTGC	1 cut(s)	751
BstC8I	GCNNGC	4 cut(s)	24, 90, 756, 1429
BstDEI	CTNAG	8 cut(s)	37, 347, 525, 1127, 1470, 1687, 1699, 1746
BstDSI	CCRYGG	3 cut(s)	192, 672, 1060
BstF5I	GGATG	3 cut(s)	250, 376, 1376
BstFNI	CGCG	3 cut(s)	90, 153, 225
BstHHI	GCGC	3 cut(s)	90, 92, 155
BstKTI	GATC	6 cut(s)	357, 1221, 1311, 1536, 1540, 1714
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
BstMCI	CGRYCG	1 cut(s)	229
BstNI	CCWGG	1 cut(s)	1509
BstNSI	RCATGY	2 cut(s)	989, 1721
BstSCI	CCNGG	2 cut(s)	143, 1507
BstSFI	CTRYAG	1 cut(s)	956
BstSLI	GKGCMC	1 cut(s)	334
BstUI	CGCG	3 cut(s)	90, 153, 225
BstV1I	GCAGC	5 cut(s)	53, 632, 865, 923, 1066
BstV2I	GAAGAC	2 cut(s)	788, 1455
BstX2I	RGATCY	2 cut(s)	1308, 1711
BstXI	CCANNNNNNTGG	1 cut(s)	1526
BstYI	RGATCY	2 cut(s)	1308, 1711
BstZI	CGGCCG	1 cut(s)	226
Bsu15I	ATCGAT	2 cut(s)	1221, 1536
BsuRI	GGCC	3 cut(s)	32, 228, 1584
BsuTUI	ATCGAT	2 cut(s)	1221, 1536
BtgI	CCRYGG	3 cut(s)	192, 672, 1060
BtgZI	GCGATG	1 cut(s)	660
BtsCI	GGATG	3 cut(s)	250, 376, 1376
BtsI	GCAGTG	2 cut(s)	749, 825
BtsIMutI	CAGTG	2 cut(s)	749, 825
BveI	ACCTGC	2 cut(s)	239, 384
Cac8I	GCNNGC	4 cut(s)	24, 90, 756, 1429
CaiI	CAGNNNCTG	2 cut(s)	751, 1253
CciI	TCATGA	2 cut(s)	589, 964
CciNI	GCGGCCGC	1 cut(s)	226
CfoI	GCGC	3 cut(s)	90, 92, 155
Cfr10I	RCCGGY	3 cut(s)	231, 803, 1691
Cfr13I	GGNCC	4 cut(s)	147, 196, 1403, 1583
ClaI	ATCGAT	2 cut(s)	1221, 1536
CseI	GACGC	1 cut(s)	1071
Csp6I	GTAC	5 cut(s)	677, 727, 1131, 1241, 1627
CviAII	CATG	9 cut(s)	193, 590, 730, 965, 986, 1061, 1704, 1718, 1756
CviQI	GTAC	5 cut(s)	677, 727, 1131, 1241, 1627
DdeI	CTNAG	8 cut(s)	37, 347, 525, 1127, 1470, 1687, 1699, 1746
DpnI	GATC	6 cut(s)	356, 1220, 1310, 1535, 1539, 1713
DpnII	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
EaeI	YGGCCR	1 cut(s)	226
EagI	CGGCCG	1 cut(s)	226
EciI	GGCGGA	1 cut(s)	41
EclXI	CGGCCG	1 cut(s)	226
Eco130I	CCWWGG	2 cut(s)	192, 1060
Eco47I	GGWCC	3 cut(s)	147, 196, 1403
Eco52I	CGGCCG	1 cut(s)	226
Eco57I	CTGAAG	5 cut(s)	303, 320, 452, 1035, 1615
EcoO109I	RGGNCCY	1 cut(s)	1403
EcoRI	GAATTC	1 cut(s)	1227
EcoRII	CCWGG	1 cut(s)	1507
EcoT14I	CCWWGG	2 cut(s)	192, 1060
ErhI	CCWWGG	2 cut(s)	192, 1060
Esp3I	CGTCTC	1 cut(s)	101
FaeI	CATG	9 cut(s)	196, 593, 733, 968, 989, 1064, 1707, 1721, 1759
FalI	AAGNNNNNCTT	2 cut(s)	1227, 1259
FaqI	GGGAC	3 cut(s)	133, 920, 1069
FatI	CATG	9 cut(s)	192, 589, 729, 964, 985, 1060, 1703, 1717, 1755
FauI	CCCGC	5 cut(s)	15, 158, 184, 190, 416
FokI	GGATG	3 cut(s)	257, 383, 1363
FspBI	CTAG	4 cut(s)	624, 1067, 1428, 1572
GlaI	GCGC	3 cut(s)	89, 91, 154
GsuI	CTGGAG	2 cut(s)	1128, 1502
HaeIII	GGCC	3 cut(s)	32, 228, 1584
HapII	CCGG	7 cut(s)	16, 145, 216, 232, 363, 804, 1692
HgaI	GACGC	1 cut(s)	1071
HhaI	GCGC	3 cut(s)	90, 92, 155
Hin1II	CATG	9 cut(s)	196, 593, 733, 968, 989, 1064, 1707, 1721, 1759
Hin6I	GCGC	3 cut(s)	88, 90, 153
HinP1I	GCGC	3 cut(s)	88, 90, 153
HincII	GTYRAC	3 cut(s)	717, 945, 1122
HindII	GTYRAC	3 cut(s)	717, 945, 1122
HindIII	AAGCTT	1 cut(s)	486
HinfI	GANTC	5 cut(s)	209, 493, 530, 554, 852
HpaII	CCGG	7 cut(s)	16, 145, 216, 232, 363, 804, 1692
Hpy166II	GTNNAC	4 cut(s)	717, 945, 1122, 1133
Hpy188I	TCNGA	4 cut(s)	12, 135, 1002, 1471
Hpy188III	TCNNGA	9 cut(s)	590, 602, 763, 833, 965, 1034, 1107, 1254, 1484
Hpy8I	GTNNAC	4 cut(s)	717, 945, 1122, 1133
Hpy99I	CGWCG	2 cut(s)	266, 269
HpyAV	CCTTC	8 cut(s)	57, 278, 344, 393, 464, 962, 1393, 1553
HpyCH4III	ACNGT	2 cut(s)	501, 1653
HpyCH4IV	ACGT	1 cut(s)	542
HpyF3I	CTNAG	8 cut(s)	37, 347, 525, 1127, 1470, 1687, 1699, 1746
HpySE526I	ACGT	1 cut(s)	542
Hsp92II	CATG	9 cut(s)	196, 593, 733, 968, 989, 1064, 1707, 1721, 1759
HspAI	GCGC	3 cut(s)	88, 90, 153
Kzo9I	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
LmnI	GCTCC	1 cut(s)	12
Lsp1109I	GCAGC	5 cut(s)	53, 632, 865, 923, 1066
LweI	GCATC	4 cut(s)	235, 898, 958, 1034
MaeI	CTAG	4 cut(s)	624, 1067, 1428, 1572
MaeII	ACGT	1 cut(s)	542
MaeIII	GTNAC	2 cut(s)	747, 1069
MalI	GATC	6 cut(s)	356, 1220, 1310, 1535, 1539, 1713
MbiI	CCGCTC	1 cut(s)	423
MboI	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
MfeI	CAATTG	1 cut(s)	516
MflI	RGATCY	2 cut(s)	1308, 1711
MhlI	GDGCHC	4 cut(s)	334, 542, 631, 811
MlyI	GAGTC	3 cut(s)	487, 539, 861
MnlI	CCTC	9 cut(s)	22, 46, 166, 420, 421, 520, 1103, 1356, 1465
MroXI	GAANNNNTTC	2 cut(s)	1366, 1465
MseI	TTAA	9 cut(s)	452, 585, 1151, 1233, 1289, 1553, 1578, 1622, 1661
MslI	CAYNNNNRTG	1 cut(s)	684
MspA1I	CMGCKG	1 cut(s)	914
MspI	CCGG	7 cut(s)	16, 145, 216, 232, 363, 804, 1692
MspR9I	CCNGG	2 cut(s)	145, 1509
MunI	CAATTG	1 cut(s)	516
MvaI	CCWGG	1 cut(s)	1509
MvnI	CGCG	3 cut(s)	90, 153, 225
NciI	CCSGG	1 cut(s)	145
NcoI	CCATGG	2 cut(s)	192, 1060
NdeII	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
NheI	GCTAGC	1 cut(s)	1427
NlaIII	CATG	9 cut(s)	196, 593, 733, 968, 989, 1064, 1707, 1721, 1759
NlaIV	GGNNCC	8 cut(s)	14, 149, 198, 361, 1183, 1363, 1404, 1728
NmuCI	GTSAC	1 cut(s)	747
NotI	GCGGCCGC	1 cut(s)	226
NspI	RCATGY	2 cut(s)	989, 1721
PagI	TCATGA	2 cut(s)	589, 964
PaqCI	CACCTGC	1 cut(s)	239
PauI	GCGCGC	1 cut(s)	88
PcsI	WCGNNNNNNNCGW	3 cut(s)	73, 209, 539
PdmI	GAANNNNTTC	2 cut(s)	1366, 1465
PfeI	GAWTC	2 cut(s)	209, 554
PflFI	GACNNNGTC	1 cut(s)	145
PflMI	CCANNNNNTGG	1 cut(s)	862
PleI	GAGTC	3 cut(s)	487, 538, 860
PpsI	GAGTC	3 cut(s)	487, 538, 860
PpuMI	RGGWCCY	1 cut(s)	1403
PshBI	ATTAAT	1 cut(s)	1622
Psp5II	RGGWCCY	1 cut(s)	1403
Psp6I	CCWGG	1 cut(s)	1507
PspGI	CCWGG	1 cut(s)	1507
PspN4I	GGNNCC	8 cut(s)	14, 149, 198, 361, 1183, 1363, 1404, 1728
PspPI	GGNCC	4 cut(s)	147, 196, 1403, 1583
PspPPI	RGGWCCY	1 cut(s)	1403
PstI	CTGCAG	1 cut(s)	960
PstNI	CAGNNNCTG	2 cut(s)	751, 1253
PsuI	RGATCY	2 cut(s)	1308, 1711
PsyI	GACNNNGTC	1 cut(s)	145
PteI	GCGCGC	1 cut(s)	88
PvuII	CAGCTG	1 cut(s)	914
RsaI	GTAC	5 cut(s)	678, 728, 1132, 1242, 1628
RsaNI	GTAC	5 cut(s)	677, 727, 1131, 1241, 1627
RseI	CAYNNNNRTG	1 cut(s)	684
SaqAI	TTAA	9 cut(s)	452, 585, 1151, 1233, 1289, 1553, 1578, 1622, 1661
Sau3AI	GATC	6 cut(s)	354, 1218, 1308, 1533, 1537, 1711
Sau96I	GGNCC	4 cut(s)	147, 196, 1403, 1583
SchI	GAGTC	3 cut(s)	487, 539, 861
ScrFI	CCNGG	2 cut(s)	145, 1509
SduI	GDGCHC	4 cut(s)	334, 542, 631, 811
SfaNI	GCATC	4 cut(s)	235, 898, 958, 1034
SfcI	CTRYAG	1 cut(s)	956
SinI	GGWCC	3 cut(s)	147, 196, 1403
SmiMI	CAYNNNNRTG	1 cut(s)	684
SmlI	CTYRAG	1 cut(s)	761
SmoI	CTYRAG	1 cut(s)	761
SspI	AATATT	1 cut(s)	1675
SspMI	CTAG	4 cut(s)	624, 1067, 1428, 1572
StyD4I	CCNGG	2 cut(s)	143, 1507
StyI	CCWWGG	2 cut(s)	192, 1060
TaaI	ACNGT	2 cut(s)	501, 1653
TaiI	ACGT	1 cut(s)	545
TaqI	TCGA	4 cut(s)	799, 1156, 1221, 1536
TatI	WGTACW	4 cut(s)	726, 1130, 1240, 1626
TauI	GCSGC	5 cut(s)	188, 228, 231, 671, 1434
TfiI	GAWTC	2 cut(s)	209, 554
Tru1I	TTAA	9 cut(s)	452, 585, 1151, 1233, 1289, 1553, 1578, 1622, 1661
Tru9I	TTAA	9 cut(s)	452, 585, 1151, 1233, 1289, 1553, 1578, 1622, 1661
TscAI	CASTG	2 cut(s)	756, 825
TseFI	GTSAC	1 cut(s)	747
TseI	GCWGC	5 cut(s)	41, 620, 878, 911, 1079
Tsp45I	GTSAC	1 cut(s)	747
TspDTI	ATGAA	5 cut(s)	99, 546, 1062, 1412, 1515
TspRI	CASTG	2 cut(s)	756, 825
Tth111I	GACNNNGTC	1 cut(s)	145
Van91I	CCANNNNNTGG	1 cut(s)	862
VpaK11BI	GGWCC	3 cut(s)	147, 196, 1403
VspI	ATTAAT	1 cut(s)	1622
XapI	RAATTY	2 cut(s)	1227, 1257
XceI	RCATGY	2 cut(s)	989, 1721
XmnI	GAANNNNTTC	2 cut(s)	1366, 1465
XspI	CTAG	4 cut(s)	624, 1067, 1428, 1572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.