Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003360

Physical Location & Seq

Genomic Coordinates Reverse (-)

14899670 .. 14907328

Gene Structure 7659 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1095300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3996 bp

ATGTACTTTCAGATCTTTGAGATTGTCAATCTGGCAAATGAGCTACTTCCTCCATTGCCACAAGGAACCATTTCTTCCAAAACTGTTTCAGCTCGTGAGAAATTATTGAATGAACAGCCTGGGCTTCTCCAGCAATTTGGAATGGATCTCCTTCCTGTTTTAATACAGATTTATGGTTCTAGCGTTAATGGCCCTGTTCGCCACAAATGTCTTTCAGTAATTGGGAAATTAATGTACTTCAGCAATGCAGAGATGATACAGTCTTTATTAAGTTCAACAAATATTTCAAGTTTCTTAGCCGGTGTCTTAGCATGGAAAGATCCGCATGTCTTGGTTCCTGCTCTCCAAATTGCTGAGATACTCATGGAGAAGCTTCCCAATACTTTCTCCAAGGTGTTTGTGAGAGAAGGTGTTGTTCATGCCGTGGACCAACTTATCTTACCTGGTACTCCAAATTCAGTTCCTGTCCCAGTATCTTCTGCTGAAAAGGATAGTGATCCTGTTCCTGGAACATCATCACGTTCTAGGCGTTATAGACGACGCAACAGTAACTCAAATCCAGATGGAAATTCATTGGAAGAAACTAAGATTCCCCCTTCAGCAAATATTGGTTCACCTCCAAGTTCAGTGGAGATTCCTACTGTTAACTCCAGCCTTCGAATGGCTGTCAGTGCATGTGCTAAAGCTTTTAAAGACAAGTACTTCCCTTCAGATCCTGGGGCTGTTGAAGTTGGAGTTACAGATGATCTATTACATTTAAAGAATCTATGCATGAAGTTGAATGCAGGTGTTGATGACCAAAAAACGAAAGCAAAGGGAAAGTCGAAAGCTTTTGGGTCTCGCTTGGTTGATAGTTCTGCAAGTAAGGAGGAGTACATGATTGGTGTGGTATCTGAGATGCTAGCCGAACTAAGCAAGGGGGATGGTGTATCCACTTTTGAGTTCATTGGTAGTGGTGTTGTTGCAGCATTGCTCAACTATTTTTCTTATGGGTACTTCTCCAAGGAGAGGATTTCGGAAGCTAATTTGCCCAAGCTTCATGAGCAAGCACTGAGAAGATTTAAGTCATTTGTTGCTGTTGCTCTTCCTTTCAGTATTAATGAAGGAAGTGTAGCACCAATGACTGTCCTCGTTCAGAAGCTCCAAAATGCTTTGTCCTCCTTAGAGCGTTTTTCCGGTTGTGCTGACTGCTGTAGAAGAGTTTCTTTGGCCTCGAGTACACAGCGAGGGGAATCTGGTCAGAAGCCTGCTGCCTCTGCTGGAAACTCGGAATCTGGAACTACACCTACAGGTGCTGGTGCTTCATCTCTGTCTACTTCAAATCCTGCTTCCACCACTCGTCGCCATTCAACGCGATCCAGAACATCTGTGAATATAGGAGATACAGCTAGGAGGGAACCATCACAAGAGAAGAGCACAAGCTCATCAAAAGGGAAGGGTAAAGCTGTTTTGAAGCCTTCTCAGGAGGAAGCAAGAGGACCTCAAACGAGAAATGCAGCTCGTAGACGGGCAGCTCTTGATAAAGATGTTCAAATGAAGCCCACAAATGGGGACACTACTTCTGAGGATGAGGAATTGGATATATCGCCTGTTGAGATGGATGAATTGGTTATTGAAGATGATGATATCTCAGATGACGAAGATGATGACCATGATGTTCTAAGAGATGACTCTCTTCCCGTTTGCATGCCGGATAAAGTACACGATGTCAAATTGGGAGACTCAACTGAGGATGCTACTGTAGCTTCAGCCACAAGTGACAATCATACTAACCCAGCTTCTGGCTCTAGTAGCAGAGCTGCTACAGGTAGAGGCTCAGATTCTGCTGAGCATAGGAGTAGTAATTCTGCTTATGGTTCAAAAGGTGCAATGTCATTTGCTGCTGCCGCCATGGCTGGGCTTGGATCTGCCAGCAGGGGCATTAGGGGAGGCAGAGATAGACAAGGACGTCCTATTTTTGGCAGTTCTAGTGATCCTCCTAAGTTGATTTTTACTTCTGGAGGGAAGCAGCTTAATAGGCATCTGACTATCTATCAGGCTATTCAGAGACAGCTTGTGCAGGATGAGGATGATGATGAAAGGTATGCTGGGAGTGATTTTGTATCCAGTGAAGGTAGCAGGCTATGGAGTGATATTTACACTATCACATACCAGAGGCCAGACAATCAAACTGACAGGGCATCTGTTGGAGGTGCAAGTCCTACAACTGCAACAAAATCTGGGAAATCTGCCTCTGCTTCCAATTCCAACTCCGAATCTCAATTGCATCGAATGTCACTTTTGGATAGTATTTTGCAGGGAGAGCTTCCTTGTGATTTAGAAAAATCTAATCCTACTTATAATATACTGGCACTACTGCGTGTACTAGAGGGCTTGAATCAGCTTGCTCCTCGCCTGAGAGCTCAGATAGTTTCCGACAGTTTTGCGGAGGGTAAAGTTTTGAATTTGGATGACTTGAGTACAACTGGTGCTAGAGTTTTTCCTGAGGAATTTATTAACAGCAAACTCACTCCCAAATTAGCCCGGCAAATTCAAGATGCCCTTGCATTGTGCAGTGGGAGTCTTCCTTCTTGGTGTTACCAGTTGACAAAAGCATGTCCATTTTTGTTTCCTTTTGAGACTCGGAGACAGTATTTCTATTCAACAGCTTTTGGATTGTCTCGCGCTCTGTACCGTCTTCAGCAGCAGCAGGGTGCTGATGGTCATGCAAATGAACGAGAGGTGAGAGTTGGGAGATTGCAACGGCAGAAAGTCCGCGTTTCCCGAAACCGGATTTTGGATTCTGCTGCAAAAGTAATGGAGATGTATTCCAGCCAAAAATCTGTGCTTGAAGTAGAATATTTTGGTGAAGTGGGCACTGGCTTGGGTCCTACGCTTGAGTTCTACACACTTTTAAGTCATGACCTACAAAGAGTTAGACTTGGCATGTGGAGGTCAAATTCTTCAATGGAGAAGACATCAATGGATATTGATGGAGATGAACCTAAAGATGGCAAAAGCAATGGCGACATTGTCCAAGCTCCGCTTGGGTTGTTCCCTCGTCCCTGGCCTCCAAACGCTGTTGCTTCTGATGGTAGCCAGTTTTCAAAAGTGATAGAATACTTTAGGTTGGTTGGGCGTGTGATGGCGAAGGCTCTTCAAGATGGGCGGCTATTGGATTTGCCACTATCAACAGCATTTTATAAGCTTTTGCTTGGTCAAGAGCTTGATTTACATGATATCCTTTCCTTTGATGCTGAGCTTGGAAAGACTCTGCAAGAATTACATAATCTTGTCTGCCGGAAACTATATTTAGAATCAAATGGTGATAATCGTGATGCCATTGTTGAATTGCATTTACGTGGAGTGTCAATTGATGACCTGTGTTTAGATTTTACACTCCCTGGCTATCCAGATTATGTGTTGAAACCTGGAGATGAAAATGTTGACATCAATAACTTGGAGGAGTACATAACTTTAGTTGTTGACGCGACAGTGAAGACTGGGATAATGAGGCAAATTGAAGCATTTAGAGCAGGGTTTAATCAGGTCTTTGACATATCGTCTTTACAAATATTTACTCCACATGAGTTGGACTATCTGCTTTGTGGTCGTCGAGAGTTGTGGGAGGCGGAGACCCTTGCTGATCATATAAAGTTTGATCATGGATACAATGCCAAGAGCCCTGCAATACTTAATTTGCTCGAGATTATGGGTGAGTTCACGCCAGAGCAGCAGCGTGCCTTCTGCCAGTTTGTTACTGGTGCACCTCGGCTTCCACCTGGTGGTTTGGCGGTGCTGAATCCAAAACTGACGATTGTGAGAAAGCATTCTTCAACCGCAAATAATGCTGCAACCAACGGGACCGGGGTTTCAGAGTTGGCAGATGATGACCTGCCCAGTGTCATGACATGCGCTAATTACCTGAAGCTTCCTCCTTATTCAACCAAGGAAATTATGTTCAAGAAATTATTGTATGCAATCAGTGAAGGGCAAGGATCCTTTGATTTGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006464 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0030154 GO:0032446 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0036211 GO:0042023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:0140096 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1332

Amino Acids

145.11

Weight (kDa)

5.69

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HECT	PF00632	1003 - 1331	1.4e-79	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38600 AT4G38600 AT4G38600
fragaria_vesca	FvH4_2g01290
malus_domestica	MD00G1173700.v1.1 MD00G1173800.v1.1 MD00G1173900.v1.1 MD05G1120900.v1.1 MD10G1095300.v1.1
prunus_persica	Prupe.8G164700_v2.0.a1
pyrus_communis	pycom05g11490 pycom05g11500 pycom10g10610 pycom10g10620
rosa_chinensis	RchiOBHm_Chr6g0251951 RchiOBHm_Chr6g0252001
rosa_laevigata	RLG00000015410 RLG00000015417
rosa_multiflora	Rmu_sc0002522.1_g000052 Rmu_sc0002522.1_g000062 Rmu_ssc0000303.1_g000016
rosa_roxburghii	Rroxscaffold_7G00216270
rosa_rugosa	Rorug05G0505300.1
rosa_samantha	Rh6AG016800 Rh6AG017300 Rh6AG017400 Rh6AG018000 Rh6AG018100 Rh6AG018900 Rh6AG019000 Rh6CG011400 Rh6DG012700
rosa_wichuraiana	Rw6G000110 Rw6G000120 Rw6G000150 Rw6G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2340, 3183
AarI	CACCTGC	1 cut(s)	776
AasI	GACNNNNNNGTC	1 cut(s)	1947
AatII	GACGTC	1 cut(s)	1951
Acc36I	ACCTGC	2 cut(s)	776, 3882
AccB7I	CCANNNNNTGG	2 cut(s)	1781, 3764
AccI	GTMKAC	2 cut(s)	1313, 1504
AccII	CGCG	4 cut(s)	1354, 2664, 2757, 3470
AciI	CCGC	9 cut(s)	323, 1887, 2426, 2755, 3023, 3148, 3611, 3773, 3819
AclWI	GGATC	9 cut(s)	153, 314, 491, 707, 1350, 1912, 1967, 3972, 3985
AcsI	RAATTY	6 cut(s)	454, 568, 2443, 2489, 2529, 2938
AcuI	CTGAAG	6 cut(s)	223, 582, 693, 1731, 2663, 3926
AcyI	GRCGYC	1 cut(s)	1948
AdeI	CACNNNGTG	1 cut(s)	3764
AhdI	GACNNNNNGTC	1 cut(s)	3541
AjnI	CCWGG	8 cut(s)	118, 442, 505, 715, 3044, 3382, 3409, 3760
AloI	GAACNNNNNNTCC	2 cut(s)	3923, 3955
Alw21I	GWGCWC	3 cut(s)	1418, 2404, 3748
Alw26I	GTCTC	7 cut(s)	843, 1713, 2041, 2612, 2620, 2664, 3608
Alw44I	GTGCAC	1 cut(s)	3744
AlwI	GGATC	9 cut(s)	153, 314, 491, 707, 1350, 1912, 1967, 3972, 3985
AlwNI	CAGNNNCTG	5 cut(s)	464, 716, 1295, 1781, 1823
Ama87I	CYCGRG	2 cut(s)	1213, 3683
AoxI	GGCC	4 cut(s)	190, 1209, 2156, 3047
ApaLI	GTGCAC	1 cut(s)	3744
ApoI	RAATTY	6 cut(s)	454, 568, 2443, 2489, 2529, 2938
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
AseI	ATTAAT	2 cut(s)	230, 1098
Asp700I	GAANNNNTTC	3 cut(s)	70, 1201, 3808
AspLEI	GCGC	2 cut(s)	2666, 3896
AspS9I	GGNCC	5 cut(s)	191, 427, 1478, 2867, 3843
AsuC2I	CCSGG	2 cut(s)	2524, 3847
AsuHPI	GGTGA	5 cut(s)	606, 2734, 2858, 3317, 3707
AsuII	TTCGAA	1 cut(s)	658
AsuNHI	GCTAGC	1 cut(s)	901
AvaI	CYCGRG	2 cut(s)	1213, 3683
AvaII	GGWCC	4 cut(s)	427, 1478, 2867, 3843
AxyI	CCTNAGG	1 cut(s)	2484
BaeGI	GKGCMC	2 cut(s)	2858, 3748
BamHI	GGATCC	1 cut(s)	3977
BanII	GRGCYC	2 cut(s)	2404, 3665
BauI	CACGAG	1 cut(s)	93
BbsI	GAAGAC	4 cut(s)	2555, 2669, 2960, 3485
Bbv12I	GWGCWC	3 cut(s)	1418, 2404, 3748
BceAI	ACGGC	2 cut(s)	407, 2759
BcgI	CGANNNNNNTGC	2 cut(s)	2405, 2439
BciT130I	CCWGG	8 cut(s)	120, 444, 507, 717, 3046, 3384, 3411, 3762
BciVI	GTATCC	3 cut(s)	940, 2113, 3641
BclI	TGATCA	2 cut(s)	3625, 3640
BcnI	CCSGG	2 cut(s)	2524, 3847
BcoDI	GTCTC	7 cut(s)	843, 1713, 2041, 2612, 2620, 2664, 3608
BfaI	CTAG	8 cut(s)	180, 525, 902, 1389, 1788, 1968, 2366, 2472
BfmI	CTRYAG	4 cut(s)	1192, 1287, 1740, 1803
BfuAI	ACCTGC	2 cut(s)	776, 3882
BfuI	GTATCC	3 cut(s)	940, 2113, 3641
BglI	GCCNNNNNGGC	1 cut(s)	1892
BglII	AGATCT	1 cut(s)	12
BlpI	GCTNAGC	2 cut(s)	1827, 3237
BmcAI	AGTACT	1 cut(s)	701
Bme18I	GGWCC	4 cut(s)	427, 1478, 2867, 3843
BmeRI	GACNNNNNGTC	1 cut(s)	3541
BmeT110I	CYCGRG	2 cut(s)	1213, 3683
BmgT120I	GGNCC	5 cut(s)	191, 427, 1478, 2867, 3843
BmiI	GGNNCC	6 cut(s)	67, 336, 1398, 2868, 3844, 3979
BmrI	ACTGGG	3 cut(s)	464, 3492, 3873
BmsI	GCATC	8 cut(s)	888, 1723, 2029, 2189, 2275, 2527, 3223, 3307
BmtI	GCTAGC	1 cut(s)	905
BmuI	ACTGGG	3 cut(s)	464, 3492, 3873
BpiI	GAAGAC	4 cut(s)	2555, 2669, 2960, 3485
BpmI	CTGGAG	4 cut(s)	113, 634, 2019, 3432
Bpu1102I	GCTNAGC	2 cut(s)	1827, 3237
Bpu14I	TTCGAA	1 cut(s)	658
BpuEI	CTTGAG	2 cut(s)	2476, 2897
BpuMI	CCSGG	2 cut(s)	2524, 3847
BsaAI	YACGTR	1 cut(s)	3341
BsaBI	GATNNNNATC	1 cut(s)	495
BsaHI	GRCGYC	1 cut(s)	1948
BsaI	GGTCTC	2 cut(s)	843, 3608
BsaWI	WCCGGW	2 cut(s)	1175, 2769
Bse118I	RCCGGY	1 cut(s)	299
Bse21I	CCTNAGG	1 cut(s)	2484
Bse3DI	GCAATG	5 cut(s)	53, 250, 968, 1875, 3007
Bse8I	GATNNNNATC	1 cut(s)	495
BseBI	CCWGG	8 cut(s)	120, 444, 507, 717, 3046, 3384, 3411, 3762
BseGI	GGATG	7 cut(s)	928, 1573, 1606, 1738, 2068, 2074, 2455
BseJI	GATNNNNATC	1 cut(s)	495
BseMI	GCAATG	5 cut(s)	53, 250, 968, 1875, 3007
BseRI	GAGGAG	3 cut(s)	884, 2379, 3458
BseSI	GKGCMC	2 cut(s)	2858, 3748
BseYI	CCCAGC	3 cut(s)	1774, 1895, 2087
BsgI	GTGCAG	2 cut(s)	2078, 2572
Bsh1236I	CGCG	4 cut(s)	1354, 2664, 2757, 3470
BshFI	GGCC	4 cut(s)	192, 1211, 2158, 3049
BsiHKAI	GWGCWC	3 cut(s)	1418, 2404, 3748
BsiHKCI	CYCGRG	2 cut(s)	1213, 3683
BsiSI	CCGG	7 cut(s)	300, 1176, 1691, 2524, 2770, 3280, 3846
BslFI	GGGAC	4 cut(s)	452, 1565, 3027, 3856
BsmAI	GTCTC	7 cut(s)	843, 1713, 2041, 2612, 2620, 2664, 3608
BsmFI	GGGAC	4 cut(s)	452, 1565, 3027, 3856
BsmI	GAATGC	2 cut(s)	787, 3808
BsnI	GGCC	4 cut(s)	192, 1211, 2158, 3049
Bso31I	GGTCTC	2 cut(s)	843, 3608
BsoBI	CYCGRG	2 cut(s)	1213, 3683
Bsp119I	TTCGAA	1 cut(s)	658
Bsp1286I	GDGCHC	5 cut(s)	1418, 2404, 2858, 3665, 3748
Bsp1720I	GCTNAGC	2 cut(s)	1827, 3237
Bsp19I	CCATGG	1 cut(s)	1890
BspACI	CCGC	9 cut(s)	323, 1887, 2426, 2755, 3023, 3148, 3611, 3773, 3819
BspANI	GGCC	4 cut(s)	192, 1211, 2158, 3049
BspFNI	CGCG	4 cut(s)	1354, 2664, 2757, 3470
BspHI	TCATGA	4 cut(s)	1039, 2899, 3885, 3992
BspLI	GGNNCC	6 cut(s)	67, 336, 1398, 2868, 3844, 3979
BspMI	ACCTGC	2 cut(s)	776, 3882
BspOI	GCTAGC	1 cut(s)	905
BspPI	GGATC	9 cut(s)	153, 314, 491, 707, 1350, 1912, 1967, 3972, 3985
BspQI	GCTCTTC	3 cut(s)	1089, 1406, 3141
BspT104I	TTCGAA	1 cut(s)	658
BspTNI	GGTCTC	2 cut(s)	843, 3608
BsrDI	GCAATG	5 cut(s)	53, 250, 968, 1875, 3007
BsrFI	RCCGGY	1 cut(s)	299
BssAI	RCCGGY	1 cut(s)	299
BssNI	GRCGYC	1 cut(s)	1948
BssSI	CACGAG	1 cut(s)	93
BssT1I	CCWWGG	4 cut(s)	390, 1002, 1890, 3927
Bst2BI	CACGAG	1 cut(s)	93
Bst2UI	CCWGG	8 cut(s)	120, 444, 507, 717, 3046, 3384, 3411, 3762
Bst6I	CTCTTC	5 cut(s)	1089, 1192, 1406, 1680, 3141
BstACI	GRCGYC	1 cut(s)	1948
BstAPI	GCANNNNNTGC	1 cut(s)	3827
BstBAI	YACGTR	1 cut(s)	3341
BstBI	TTCGAA	1 cut(s)	658
BstC8I	GCNNGC	8 cut(s)	903, 1047, 1248, 1688, 1912, 2120, 2385, 3720
BstDSI	CCRYGG	2 cut(s)	423, 1890
BstF5I	GGATG	7 cut(s)	928, 1573, 1606, 1738, 2068, 2074, 2455
BstFNI	CGCG	4 cut(s)	1354, 2664, 2757, 3470
BstHHI	GCGC	2 cut(s)	2666, 3896
BstMAI	GTCTC	7 cut(s)	843, 1713, 2041, 2612, 2620, 2664, 3608
BstNI	CCWGG	8 cut(s)	120, 444, 507, 717, 3046, 3384, 3411, 3762
BstNSI	RCATGY	6 cut(s)	329, 678, 1690, 2598, 2929, 3894
BstSFI	CTRYAG	4 cut(s)	1192, 1287, 1740, 1803
BstSLI	GKGCMC	2 cut(s)	2858, 3748
BstUI	CGCG	4 cut(s)	1354, 2664, 2757, 3470
BstV2I	GAAGAC	4 cut(s)	2555, 2669, 2960, 3485
BstX2I	RGATCY	6 cut(s)	12, 145, 319, 712, 1904, 3977
BstXI	CCANNNNNNTGG	1 cut(s)	137
BstYI	RGATCY	6 cut(s)	12, 145, 319, 712, 1904, 3977
Bsu36I	CCTNAGG	1 cut(s)	2484
BsuI	GTATCC	3 cut(s)	940, 2113, 3641
BsuRI	GGCC	4 cut(s)	192, 1211, 2158, 3049
BtgI	CCRYGG	2 cut(s)	423, 1890
BtsCI	GGATG	7 cut(s)	928, 1573, 1606, 1738, 2068, 2074, 2455
BtsI	GCAGTG	1 cut(s)	2560
BtsIMutI	CAGTG	9 cut(s)	633, 676, 1049, 2113, 2560, 2856, 3480, 3886, 3970
BveI	ACCTGC	2 cut(s)	776, 3882
Cac8I	GCNNGC	8 cut(s)	903, 1047, 1248, 1688, 1912, 2120, 2385, 3720
CaiI	CAGNNNCTG	5 cut(s)	464, 716, 1295, 1781, 1823
CciI	TCATGA	4 cut(s)	1039, 2899, 3885, 3992
CfoI	GCGC	2 cut(s)	2666, 3896
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	5 cut(s)	191, 427, 1478, 2867, 3843
CseI	GACGC	2 cut(s)	549, 3476
CsiI	ACCWGGT	2 cut(s)	442, 3760
CspCI	CAANNNNNGTGG	4 cut(s)	609, 644, 3552, 3587
DraI	TTTAAA	2 cut(s)	691, 759
DraIII	CACNNNGTG	1 cut(s)	3764
DrdI	GACNNNNNNGTC	1 cut(s)	1947
DriI	GACNNNNNGTC	1 cut(s)	3541
DseDI	GACNNNNNNGTC	1 cut(s)	1947
Eam1104I	CTCTTC	5 cut(s)	1089, 1192, 1406, 1680, 3141
Eam1105I	GACNNNNNGTC	1 cut(s)	3541
EarI	CTCTTC	5 cut(s)	1089, 1192, 1406, 1680, 3141
EciI	GGCGGA	1 cut(s)	3626
Ecl136II	GAGCTC	1 cut(s)	2402
Eco130I	CCWWGG	4 cut(s)	390, 1002, 1890, 3927
Eco24I	GRGCYC	2 cut(s)	2404, 3665
Eco31I	GGTCTC	2 cut(s)	843, 3608
Eco32I	GATATC	2 cut(s)	1627, 3220
Eco47I	GGWCC	4 cut(s)	427, 1478, 2867, 3843
Eco53kI	GAGCTC	1 cut(s)	2402
Eco57I	CTGAAG	6 cut(s)	223, 582, 693, 1731, 2663, 3926
Eco81I	CCTNAGG	1 cut(s)	2484
Eco88I	CYCGRG	2 cut(s)	1213, 3683
EcoICRI	GAGCTC	1 cut(s)	2402
EcoO109I	RGGNCCY	2 cut(s)	1478, 2867
EcoRII	CCWGG	8 cut(s)	118, 442, 505, 715, 3044, 3382, 3409, 3760
EcoRV	GATATC	2 cut(s)	1627, 3220
EcoT14I	CCWWGG	4 cut(s)	390, 1002, 1890, 3927
EcoT22I	ATGCAT	1 cut(s)	773
EcoT38I	GRGCYC	2 cut(s)	2404, 3665
ErhI	CCWWGG	4 cut(s)	390, 1002, 1890, 3927
FalI	AAGNNNNNCTT	6 cut(s)	1189, 1221, 2526, 2558, 3009, 3041
FaqI	GGGAC	4 cut(s)	452, 1565, 3027, 3856
FbaI	TGATCA	2 cut(s)	3625, 3640
FblI	GTMKAC	2 cut(s)	1313, 1504
FokI	GGATG	7 cut(s)	935, 1580, 1613, 1745, 2075, 2081, 2462
FriOI	GRGCYC	2 cut(s)	2404, 3665
FspBI	CTAG	8 cut(s)	180, 525, 902, 1389, 1788, 1968, 2366, 2472
GlaI	GCGC	2 cut(s)	2665, 3895
GsaI	CCCAGC	3 cut(s)	1778, 1899, 2091
GsuI	CTGGAG	4 cut(s)	113, 634, 2019, 3432
HaeIII	GGCC	4 cut(s)	192, 1211, 2158, 3049
HapII	CCGG	7 cut(s)	300, 1176, 1691, 2524, 2770, 3280, 3846
HgaI	GACGC	2 cut(s)	549, 3476
HhaI	GCGC	2 cut(s)	2666, 3896
Hin1I	GRCGYC	1 cut(s)	1948
Hin6I	GCGC	2 cut(s)	2664, 3894
HinP1I	GCGC	2 cut(s)	2664, 3894
HincII	GTYRAC	4 cut(s)	646, 2586, 3427, 3466
HindII	GTYRAC	4 cut(s)	646, 2586, 3427, 3466
HindIII	AAGCTT	6 cut(s)	371, 684, 828, 1034, 3185, 3908
HpaI	GTTAAC	1 cut(s)	646
HpaII	CCGG	7 cut(s)	300, 1176, 1691, 2524, 2770, 3280, 3846
HphI	GGTGA	5 cut(s)	606, 2734, 2858, 3317, 3707
Hpy99I	CGWCG	3 cut(s)	543, 1344, 3597
HpyCH4IV	ACGT	3 cut(s)	520, 1948, 3340
HpySE526I	ACGT	3 cut(s)	520, 1948, 3340
Hsp92I	GRCGYC	1 cut(s)	1948
HspAI	GCGC	2 cut(s)	2664, 3894
Ksp22I	TGATCA	2 cut(s)	3625, 3640
KspAI	GTTAAC	1 cut(s)	646
LguI	GCTCTTC	3 cut(s)	1089, 1406, 3141
LmnI	GCTCC	3 cut(s)	1146, 2392, 3025
LweI	GCATC	8 cut(s)	888, 1723, 2029, 2189, 2275, 2527, 3223, 3307
MabI	ACCWGGT	2 cut(s)	442, 3760
MaeI	CTAG	8 cut(s)	180, 525, 902, 1389, 1788, 1968, 2366, 2472
MaeII	ACGT	3 cut(s)	520, 1948, 3340
MaeIII	GTNAC	6 cut(s)	548, 736, 1757, 2274, 2576, 3736
MfeI	CAATTG	2 cut(s)	2261, 3351
MflI	RGATCY	6 cut(s)	12, 145, 319, 712, 1904, 3977
MhlI	GDGCHC	5 cut(s)	1418, 2404, 2858, 3665, 3748
MlyI	GAGTC	5 cut(s)	1664, 1715, 2569, 2614, 3244
MmeI	TCCRAC	5 cut(s)	712, 2167, 2271, 2439, 3552
Mph1103I	ATGCAT	1 cut(s)	773
MroXI	GAANNNNTTC	3 cut(s)	70, 1201, 3808
MslI	CAYNNNNRTG	2 cut(s)	2709, 3339
MspI	CCGG	7 cut(s)	300, 1176, 1691, 2524, 2770, 3280, 3846
MunI	CAATTG	2 cut(s)	2261, 3351
Mva1269I	GAATGC	2 cut(s)	787, 3808
MvaI	CCWGG	8 cut(s)	120, 444, 507, 717, 3046, 3384, 3411, 3762
MvnI	CGCG	4 cut(s)	1354, 2664, 2757, 3470
NciI	CCSGG	2 cut(s)	2524, 3847
NcoI	CCATGG	1 cut(s)	1890
NheI	GCTAGC	1 cut(s)	901
NlaIV	GGNNCC	6 cut(s)	67, 336, 1398, 2868, 3844, 3979
NmeAIII	GCCGAG	1 cut(s)	3730
NmuCI	GTSAC	2 cut(s)	1757, 2274
NsiI	ATGCAT	1 cut(s)	773
NspI	RCATGY	6 cut(s)	329, 678, 1690, 2598, 2929, 3894
NspV	TTCGAA	1 cut(s)	658
PaeI	GCATGC	1 cut(s)	1690
PaeR7I	CTCGAG	2 cut(s)	1213, 3683
PagI	TCATGA	4 cut(s)	1039, 2899, 3885, 3992
PaqCI	CACCTGC	1 cut(s)	776
PciSI	GCTCTTC	3 cut(s)	1089, 1406, 3141
PcsI	WCGNNNNNNNCGW	1 cut(s)	526
PctI	GAATGC	2 cut(s)	787, 3808
PdmI	GAANNNNTTC	3 cut(s)	70, 1201, 3808
PflFI	GACNNNGTC	1 cut(s)	3011
PflMI	CCANNNNNTGG	2 cut(s)	1781, 3764
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	5 cut(s)	1664, 1715, 2568, 2614, 3244
PpsI	GAGTC	5 cut(s)	1664, 1715, 2568, 2614, 3244
Ppu21I	YACGTR	1 cut(s)	3341
PpuMI	RGGWCCY	2 cut(s)	1478, 2867
PshBI	ATTAAT	2 cut(s)	230, 1098
PsiI	TTATAA	2 cut(s)	2340, 3183
Psp124BI	GAGCTC	1 cut(s)	2404
Psp5II	RGGWCCY	2 cut(s)	1478, 2867
Psp6I	CCWGG	8 cut(s)	118, 442, 505, 715, 3044, 3382, 3409, 3760
PspFI	CCCAGC	3 cut(s)	1774, 1895, 2087
PspGI	CCWGG	8 cut(s)	118, 442, 505, 715, 3044, 3382, 3409, 3760
PspN4I	GGNNCC	6 cut(s)	67, 336, 1398, 2868, 3844, 3979
PspPI	GGNCC	5 cut(s)	191, 427, 1478, 2867, 3843
PspPPI	RGGWCCY	2 cut(s)	1478, 2867
PspXI	VCTCGAGB	1 cut(s)	1213
PsrI	GAACNNNNNNTAC	2 cut(s)	1270, 1302
PstNI	CAGNNNCTG	5 cut(s)	464, 716, 1295, 1781, 1823
PsuI	RGATCY	6 cut(s)	12, 145, 319, 712, 1904, 3977
PsyI	GACNNNGTC	1 cut(s)	3011
RseI	CAYNNNNRTG	2 cut(s)	2709, 3339
SacI	GAGCTC	1 cut(s)	2404
SapI	GCTCTTC	3 cut(s)	1089, 1406, 3141
Sau96I	GGNCC	5 cut(s)	191, 427, 1478, 2867, 3843
ScaI	AGTACT	1 cut(s)	701
SchI	GAGTC	5 cut(s)	1664, 1715, 2569, 2614, 3244
SduI	GDGCHC	5 cut(s)	1418, 2404, 2858, 3665, 3748
SexAI	ACCWGGT	2 cut(s)	442, 3760
SfaNI	GCATC	8 cut(s)	888, 1723, 2029, 2189, 2275, 2527, 3223, 3307
SfcI	CTRYAG	4 cut(s)	1192, 1287, 1740, 1803
Sfr274I	CTCGAG	2 cut(s)	1213, 3683
SfuI	TTCGAA	1 cut(s)	658
SinI	GGWCC	4 cut(s)	427, 1478, 2867, 3843
SlaI	CTCGAG	2 cut(s)	1213, 3683
SmiMI	CAYNNNNRTG	2 cut(s)	2709, 3339
SmlI	CTYRAG	4 cut(s)	1213, 2455, 2876, 3683
SmoI	CTYRAG	4 cut(s)	1213, 2455, 2876, 3683
SphI	GCATGC	1 cut(s)	1690
SsiI	CCGC	9 cut(s)	323, 1887, 2426, 2755, 3023, 3148, 3611, 3773, 3819
SspI	AATATT	4 cut(s)	283, 607, 2840, 3555
SspMI	CTAG	8 cut(s)	180, 525, 902, 1389, 1788, 1968, 2366, 2472
SstI	GAGCTC	1 cut(s)	2404
StyI	CCWWGG	4 cut(s)	390, 1002, 1890, 3927
TaiI	ACGT	3 cut(s)	523, 1951, 3343
TaqI	TCGA	6 cut(s)	658, 824, 1214, 2269, 3595, 3684
TatI	WGTACW	9 cut(s)	3, 234, 699, 873, 1217, 1699, 2362, 2459, 3447
TauI	GCSGC	2 cut(s)	1889, 3151
TscAI	CASTG	9 cut(s)	633, 676, 1056, 2113, 2560, 2863, 3480, 3886, 3970
TseFI	GTSAC	2 cut(s)	1757, 2274
Tsp45I	GTSAC	2 cut(s)	1757, 2274
TspRI	CASTG	9 cut(s)	633, 676, 1056, 2113, 2560, 2863, 3480, 3886, 3970
Tth111I	GACNNNGTC	1 cut(s)	3011
Van91I	CCANNNNNTGG	2 cut(s)	1781, 3764
VneI	GTGCAC	1 cut(s)	3744
VpaK11BI	GGWCC	4 cut(s)	427, 1478, 2867, 3843
VspI	ATTAAT	2 cut(s)	230, 1098
XapI	RAATTY	6 cut(s)	454, 568, 2443, 2489, 2529, 2938
XceI	RCATGY	6 cut(s)	329, 678, 1690, 2598, 2929, 3894
XcmI	CCANNNNNNNNNTGG	3 cut(s)	658, 3023, 3737
XhoI	CTCGAG	2 cut(s)	1213, 3683
XmiI	GTMKAC	2 cut(s)	1313, 1504
XmnI	GAANNNNTTC	3 cut(s)	70, 1201, 3808
XspI	CTAG	8 cut(s)	180, 525, 902, 1389, 1788, 1968, 2366, 2472
ZraI	GACGTC	1 cut(s)	1949
ZrmI	AGTACT	1 cut(s)	701
Zsp2I	ATGCAT	1 cut(s)	773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.