Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003360

Physical Location & Seq

Genomic Coordinates Forward (+)

24355470 .. 24364864

Gene Structure 9395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1120900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5655 bp

ATGGAAACTCGGAGCCGGAAGCGGGCGGAGGCCACCTCAGCAGCCCCTTCTTCTTCGTCTGGTCGTACTACTCGTTCTTCCAAGCGCGCTCGTCTTTCTGCTTCTACATTGTCTTCTATTGGCCCCGCTTCGATTACGACCCGGTCCCGCGCTTCTCGGACCCAAAACGAACCCTCACCTGCCGCACCCATGGACCCCACGAACGAATCGTCCGGCTCGCGCGGCCGCCGCAACAAGAGTTCCGATAAGGATGGCTCGGATAAAGGTAAGGAGAAGGAGCATGAGGTTAGGATTAGGGATAGGGAGAGGGAGAGGGAAAGAGAGAGGGAAGCTGAGAGGAACCTAGGGTTGAATATGGATGGTGGAGGAACTGGCGATGACGATGACAATGATAGTGAAGGCGGTGTTGGGATTTTGCACCAGAACTTGACTTCAGCGAGTAGTGCCCTTCAGGGGCTTCTTAGGAAGATCGGCGCCGGATTGGACGACCTGCTTCCTTCTTCGGCTATGGGTTCGGCTTCTTCATCGCACCAGAGCGGGAGGCTGAAGAAGATATTATCTGGGTTAAGAGCTGATGGGGAAGAAGGGAAACAGGTGGAAGCTCTGACGCAGCTCTGTGAAATGCTTTCAATTGGAACTGAGGAGTCGTTGAGCACGTTTTCGGTTGATTCATTCGTGCCTGTACTTGTGGGGTTGCTTAATCATGAGAGCAATCCAGATATTATGCTCCTTGCAGCTAGAGCACTCACTCATCTTTGTGATGTGCTTCCATCATCTTGTGCCGCTGTGGTGCATTATGGTGCTGTTTCTTGCTTTTGTGCAAGGTTGTTGACGATTGAGTACATGGACTTGGCAGAGCAGTCACTGCAAGCTCTCAAGAAAATATCACAGGAACACCCAACTGCCTGTCTTCGAGCGGGTGCTCTTATGGCAGTGCTTTCTTATTTGGATTTTTTCTCCACTGGAGTCCAGCGTGTGGCATTATCAACTGCTGCGAATATGTGCAAGAAACTTCCTTTAGATGCAGCTGACTTTGTGATGGAAGCTGTCCCATTGCTGACAAACCTTCTGCAGTATCATGATGCAAAGGTTTTGGAGCATGCTTCTGTATGTCTGACACGAATTGCTGAAGCTTTTGCATCATCTCCTGACAAACTTGACGAATTATGCAACCATGGGCTAGTTACTCAAGCTGCGTCTCTTATTTCCACCAGCAATTCTGGAGGTGCCCAGTCAACTCTCAGTACACCTACTTATACTGGTTTAATTCGGCTACTATCAACCTGTGCAAGTGGTTCCCCATTAGGGTCTAAAACTTTACTTCTTCTTGGGATCAGTGGAATTCTTAAAGATGTACTTTCAGGTTCTGGAATTTCTTCTGGCACGACAGTTTCTCCTGCTTTAAGTAAGCCACCGGAGCAGATCTTTGAGATTGTCAATCTGGCAAATGAGCTACTTCCTCCTTTACCACAAGGAACCATTTCCATTCCGTCCAGCTTCAATGTATTTATGAAAGGTCCTGTTGTGAAAAAGTCATCTGCTAGCGCTTCTGGGAAACAAGAAGACACCAACGGAAATGGTTCTGAGGTTTCAGCTCGCGAGAAATTATTGAATGAACAACCTTCCCTTCTCCAGCAATTTGGAATGGATCTCCTTCCTGTTCTAATACAGATATATGGTTCTAGTGTTAATGGCCCTGTTCGCCACAAATGTCTTTCAGTAATTGGGAAATTAATGTACTTCAGCAATGCAGAGATGATACAGTCATTATTAAGTTCGACAAATATTTCAAGTTTCTTAGCCGGTGTCTTAGCATGGAAAGATCCGCACGTCTTGGTTCCTGCTCTCCAAGTTGCTGAGATACTCATGGAGAAGCTTCCCAATACTTTCTCCAAGGTGTTTGTGAGAGAAGGTGTTGTTCATGCTGTGGACCAACTTATCTTACCTAGTACTCCAAATTCAGTTCCTGCCTCAGTATCTTCTGCTGAAAAGGATAGTGATCCTGTTTCTGGAACATCATCACGGTCTAGGCGTTATAGACGACGCAACAGTAACCCAATTCCAGATGGAAATTCATTGGAAGAAACCAAGAGTCCTGCTTCAGCAAATATTGGTTCACCTCCAAGTTCAGTGGAGATTCCTACTGTGAGCTCCAGCCTTCGAATCGCTGTCAGTGCATGTGCTAAAGCTTTTAAAGACAAGTACTTCCCCTCAGATCCTGGGGCTGTTGAAATTGGAGTTACAGATGATCTATTACATTTAAAGAATCTATGCATGAAGTTGAATGCCGGTGTTGATGACCAAAAAACGAAAGCAAAGGGAAAGTCGAAAGCATCTGGGTCTCGCTTGATTGATAGTTCTGCAAGCAAGGAGGAGTACTTGACTGGTGTCGTATATGAGATGCTAGCCGAACTAAGCAAAGGGGACGGTGTATCCACTTTTGAGTTCATTGGTAGTGGTGTTGTTGCAGCGTTGCTTAACTATTTTTCTTGTGGGTACTTTTCCAAGGAGAGGATTTCAGAAGCTAATTTGCCCAAGCTTCGTGAGCAAGCCCTTAAAAGATTTAAGTCATTTGTTGCTGTTGCTCTTCCTTTGAGTATTGACGAAGGAAGTGTAGCACTGATGACTGTCCTTGTTCAGAAGCTCCAAAATGCTTTGTCCTCCTTAGAGCGTTTTCCTGTCGTGCTGAGTCATTCATCTAGGTCATCCACTGGGAGTGCACGCCTCTCCTCTGGACTTAGTGCATTATCTCAGCCCTTTAAGTTGCGTCTTTGTCGTGCACAAGGAGAAAAGACCCTCCGAGACTATTCATCAAACGTCGTTCTGATTGATCCATTAGCAAGTTTAGCTGCTGTAGAAGAGTTTCTTTGGCCTCGAGTTCAGCGAGGTGAATCTGGTCAGAAGCCTGCTGCATCTGCTGGAAACTCGGAATCTGGAGCTACACCTACAGGTGCTGGTGCTTCATCTCTGCCTACTTCAAATTCTGCTTCCTCCACTCGTCGCCATTCAACGCGGTCAAGAACATCTGTGAATATAGGAGATACAGGTAGGAGGGAACCATCACAAGAGAAGAACACAAGCTCATCAAAAGGGAAGGGGAAAGCTGTTTTGAAGCCTTCTCAGGAGGAAGCAAGAGGAACTCAAACGAGAAATGCAGCTCGTCGACGAGCCGCTCTTGATAAAGAAGTTCAAATGAAGCCTGCAAATGGGGACACTACTTCTGAGGATGAGGAATTGGATATATCGCCCGTTGAGATGGATGAATTGGTCATTGAAGATGATGATATCTCAGATGACGAAGATGATGACCATGATGTTCTAAGAGATGACTCTCTTCCTGTTTGCATGCCGGATAAAGTACATGATGTGAAATTGGGAGACTCAACTGAGGATGCTACTGTAGCTTCCGCCACGAGTGACACCCACACTATCCCAGCTCCTGGCTCTAGTAGCAGAGCTGCTACGGTTAGAGGCTCAGATTATGCCGAACATAGGAGTAGTAATTCTTATGGTTCAAAGGGTGCAATGTCATTTGCTGCTGCCGCCATGGCTGGGCTTGGATCTGCCAGCAGGGGCATCAGGGGAGGCAGGGATCGACAAGGACATCCCATTTTTGGCATTTCTAGTGATCCTCCTAAGTTGATTTTTACTTCTGGAGGGAAGCAGCTTAATAGGCATCTGACTATCTATCAGGCTATTCAGAGACAGCTTGTGCAAGATGAGGATGATGATGAAAGGTATGCTGGCAGTGATTTCGTATCCAGTGATGGTAGCAGGCTGTGGAGTGATATTTACACTATCACATACCAGAGGCCAGACAATCAAGCTGACAGGGCATCTGTTGGAGGTGCAAGTTCTATGACTTCAACAAAATCTTGCAAATCTGGATCTGCTTCCAATTCCAACTCCGACTCCCAATTGCTTCAAATGTCACTTTTGGATAGCATTTTGCAGGGAGAGCTTCCTTGTGATTTAGAAAAATCTAATCCTACTTACAATATTGTGGCACTACTCCATGTACTAGAGGGCTTGAATCAGCTTGCACCTCGCCTAAGAGCTCAGATAGTTTCCGACAGTTTTGCTGAGGGTAAAGTTTTGAATATGGATGACTTGAGTACAACTGGTGCTAAGGTTTTTCCTGAAGAATTTGTTAACAGCAAACTCACTCCCAAATTAGCCCGACAAATTCAAGATGCCCTTGCGTTGTGCAGTGGGAGTCTTCCTTCTTGGTGTTACCAGTTGACAAAAGCATGTCCATTTTTGTTTCCTTTTGAGACTCGGAGACAGTATTTCTATTCAACGGCTTTTGGATTGTCCCGCGCTTTGCACCGTCTTCAGCAGCAGCAGGGTGCTGATGGTCATGGTGCAAATGAACGAGAGGTGAGAGTTGGGAGATTGCAACGACAGAAAGTTCGTGTGTCCCGAAACCGGATTTTGGATTCTGCTGCAAAAGTAATGGAGATGTATTCCAGCCAAAAATCTGTGCTTGAAGTAGAATATTTCGGTGAAGTGGGCACTGGCTTGGGTCCTACACTTGAGTTTTACACACTTTTAAGTCATGACCTACAAAGAGTTAGACTGGGCATGTGGAGATCGAATTCTTCCATGGAGAAAACATCAATGGATATTGATGGAGATGAACATAAAGATGGAAAAAGCAATGGTGACATTGTCCAAGCTCCTCTTGGGTTGTTCCCACGTCCCTGGCCTCCAAATGCTGTTGCTTCTGATGGTAGCCAATTTTCAAAAGTCATAGAATACTTTAGGTTGGTTGGGCGTGTGATGGCTAAGGCTCTTCAAGATGGGCGGCTATTGGACTTGCCACTATCAACAGCATTTTATAAGCTTTTGCTTGGTCAAGAGCTTGATTTACATGATGTTCTTTCCTTTGATGCTGAGCTTGGAAAGACTCTGCAGGAATTACATAACCTTGTCTGCCGGAAACTGTATTTAGAATCAAATGGTGATAATTGTGATGCCATTGCTGAATTGCGTTTCCGTGGAGCATCAGTGGATGACCTCTGTTTAGATTTTACACTTCCTGGCTATCCAGATTATGTGTTGAAACCGGGAGATGAAAATGTTGATATCAATAACTTGGAGGAGTATATATCTTTTGTTGTTGACGCGACAGTGAAGACTGGGATAATGAGGCAAATTGAAGCATTTAGAGCCGGGTTTAATCAGGTCTTTGACTTATCATCTTTACAAATATTTACTCCACATGAGTTGGACTACCTGCTTTGTGGCCGTAGAGAGTTGTGGGAGGCAGATACCCTTGCTGATCATATAAAGTTTGATCATGGATACAATGCCAAGAGCCCTGCAATACTTAATTTGCTCGAGATTATGGGTGAGTTCACCCCAGAGCAGCAGCGCGCTTTCTGCCAGTTTGTTACTGGTGCACCTCGGCTTCCACCTGGTGGTTTGGCGGTGCTGAATCCAAAACTGACCATTGTGAGAAAGCATTCTTCGACCGCAAATAATGCTGCAATCAACGGGACCGGGGTTTCAGAGTTGGCAGATGATGACCTGCCCAGTGTCATGACATGCGCTAATTACCTGAAGCTTCCTCCATATTCAACCAAGGAAATTATGTTCAAGAAACTATTGTATGCAATCAGTGAAGGCCAAGGATCCTTTGATTTGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006464 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0030154 GO:0032446 GO:0032502 GO:0032989 GO:0034641 GO:0034645 GO:0036211 GO:0042023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044786 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:0140096 GO:1901360 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1885

Amino Acids

203.1

Weight (kDa)

5.85

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	174 - 305	2.4e-50	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT	PF00632	1551 - 1884	2.4e-78	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38600 AT4G38600 AT4G38600
fragaria_vesca	FvH4_2g01290
malus_domestica	MD00G1173700.v1.1 MD00G1173800.v1.1 MD00G1173900.v1.1 MD05G1120900.v1.1 MD10G1095300.v1.1
prunus_persica	Prupe.8G164700_v2.0.a1
pyrus_communis	pycom05g11490 pycom05g11500 pycom10g10610 pycom10g10620
rosa_chinensis	RchiOBHm_Chr6g0251951 RchiOBHm_Chr6g0252001
rosa_laevigata	RLG00000015410 RLG00000015417
rosa_multiflora	Rmu_sc0002522.1_g000052 Rmu_sc0002522.1_g000062 Rmu_ssc0000303.1_g000016
rosa_roxburghii	Rroxscaffold_7G00216270
rosa_rugosa	Rorug05G0505300.1
rosa_samantha	Rh6AG016800 Rh6AG017300 Rh6AG017400 Rh6AG018000 Rh6AG018100 Rh6AG018900 Rh6AG019000 Rh6CG011400 Rh6DG012700
rosa_wichuraiana	Rw6G000110 Rw6G000120 Rw6G000150 Rw6G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4842
AarI	CACCTGC	1 cut(s)	187
Acc36I	ACCTGC	4 cut(s)	187, 498, 5247, 5541
AccB1I	GGYRCC	2 cut(s)	473, 1226
AccB7I	CCANNNNNTGG	3 cut(s)	976, 3440, 5423
AccBSI	CCGCTC	3 cut(s)	537, 917, 3173
AccI	GTMKAC	1 cut(s)	3163
AccII	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
AcoI	YGGCCR	2 cut(s)	223, 5248
AcsI	RAATTY	8 cut(s)	1341, 1371, 1957, 2071, 2980, 4145, 4185, 4597
AcuI	CTGAAG	9 cut(s)	417, 434, 566, 1149, 1726, 2085, 4161, 4319, 5585
AcyI	GRCGYC	1 cut(s)	474
AdeI	CACNNNGTG	1 cut(s)	5423
AfeI	AGCGCT	1 cut(s)	1546
AjiI	CACGTC	2 cut(s)	1831, 4700
AjnI	CCWGG	5 cut(s)	2218, 3439, 4703, 5041, 5419
AjuI	GAANNNNNNNTTGG	2 cut(s)	390, 422
AloI	GAACNNNNNNTCC	4 cut(s)	194, 226, 5582, 5614
Alw21I	GWGCWC	8 cut(s)	656, 745, 925, 2153, 2722, 2782, 4060, 5407
Alw26I	GTCTC	7 cut(s)	1203, 2346, 2796, 3372, 3697, 4268, 4276
Alw44I	GTGCAC	3 cut(s)	2718, 2778, 5403
AlwNI	CAGNNNCTG	6 cut(s)	865, 1367, 1967, 2219, 2954, 3440
Ama87I	CYCGRG	2 cut(s)	2874, 5342
Aor51HI	AGCGCT	1 cut(s)	1546
AoxI	GGCC	9 cut(s)	30, 121, 223, 1693, 2870, 3812, 4706, 5248, 5629
ApaLI	GTGCAC	3 cut(s)	2718, 2778, 5403
ApoI	RAATTY	8 cut(s)	1341, 1371, 1957, 2071, 2980, 4145, 4185, 4597
ArsI	GACNNNNNNTTYG	6 cut(s)	194, 226, 2309, 2341, 4395, 4427
AseI	ATTAAT	1 cut(s)	1733
Asp700I	GAANNNNTTC	5 cut(s)	1375, 1480, 1579, 2862, 5467
AspA2I	CCTAGG	1 cut(s)	343
AspS9I	GGNCC	9 cut(s)	122, 144, 159, 193, 1517, 1694, 1930, 4526, 5502
AsuC2I	CCSGG	4 cut(s)	142, 5070, 5176, 5506
AsuHPI	GGTGA	9 cut(s)	168, 2109, 2900, 4393, 4517, 4676, 4976, 5353, 5366
AsuII	TTCGAA	1 cut(s)	2161
AsuNHI	GCTAGC	2 cut(s)	1541, 2404
AvaI	CYCGRG	2 cut(s)	2874, 5342
AvaII	GGWCC	7 cut(s)	144, 159, 193, 1517, 1930, 4526, 5502
AvrII	CCTAGG	1 cut(s)	343
BaeGI	GKGCMC	6 cut(s)	448, 1231, 2722, 2782, 4517, 5407
BamHI	GGATCC	1 cut(s)	5636
BanI	GGYRCC	2 cut(s)	473, 1226
BanII	GRGCYC	3 cut(s)	2153, 4060, 5324
BauI	CACGAG	1 cut(s)	3412
BbsI	GAAGAC	6 cut(s)	105, 902, 1569, 4211, 4325, 5144
Bbv12I	GWGCWC	8 cut(s)	656, 745, 925, 2153, 2722, 2782, 4060, 5407
BbvCI	CCTCAGC	2 cut(s)	37, 4083
BceAI	ACGGC	2 cut(s)	4317, 5235
BcgI	CGANNNNNNTGC	4 cut(s)	3736, 3770, 4061, 4095
BciT130I	CCWGG	5 cut(s)	2220, 3441, 4705, 5043, 5421
BciVI	GTATCC	3 cut(s)	2443, 3769, 5300
BclI	TGATCA	2 cut(s)	5284, 5299
BcnI	CCSGG	4 cut(s)	142, 5070, 5176, 5506
BcoDI	GTCTC	7 cut(s)	1203, 2346, 2796, 3372, 3697, 4268, 4276
BfmI	CTRYAG	5 cut(s)	1070, 2853, 2946, 3399, 4913
BfoI	RGCGCY	2 cut(s)	477, 1548
BfuAI	ACCTGC	4 cut(s)	187, 498, 5247, 5541
BfuI	GTATCC	3 cut(s)	2443, 3769, 5300
BglI	GCCNNNNNGGC	1 cut(s)	3548
BglII	AGATCT	1 cut(s)	1422
BlnI	CCTAGG	1 cut(s)	343
BlpI	GCTNAGC	1 cut(s)	4896
BmcAI	AGTACT	3 cut(s)	1951, 2204, 2378
Bme1390I	CCNGG	9 cut(s)	142, 2220, 3441, 4705, 5043, 5070, 5176, 5421, 5506
Bme18I	GGWCC	7 cut(s)	144, 159, 193, 1517, 1930, 4526, 5502
BmeT110I	CYCGRG	2 cut(s)	2874, 5342
BmgBI	CACGTC	2 cut(s)	1831, 4700
BmgT120I	GGNCC	9 cut(s)	122, 144, 159, 193, 1517, 1694, 1930, 4526, 5502
BmrFI	CCNGG	9 cut(s)	142, 2220, 3441, 4705, 5043, 5070, 5176, 5421, 5506
BmrI	ACTGGG	5 cut(s)	1225, 2721, 4589, 5151, 5532
BmtI	GCTAGC	2 cut(s)	1545, 2408
BmuI	ACTGGG	5 cut(s)	1225, 2721, 4589, 5151, 5532
BoxI	GACNNNNGTC	1 cut(s)	2387
BpiI	GAAGAC	6 cut(s)	105, 902, 1569, 4211, 4325, 5144
BplI	GAGNNNNNCTC	2 cut(s)	20, 52
BpmI	CTGGAG	6 cut(s)	984, 1242, 1616, 2137, 2955, 3675
Bpu10I	CCTNAGC	4 cut(s)	37, 4083, 4128, 4788
Bpu1102I	GCTNAGC	1 cut(s)	4896
Bpu14I	TTCGAA	1 cut(s)	2161
BpuEI	CTTGAG	4 cut(s)	860, 1173, 4132, 4556
BpuMI	CCSGG	4 cut(s)	142, 5070, 5176, 5506
BsaBI	GATNNNNATC	1 cut(s)	1998
BsaHI	GRCGYC	1 cut(s)	474
BsaI	GGTCTC	1 cut(s)	2346
BsaWI	WCCGGW	2 cut(s)	1414, 4428
BsaXI	ACNNNNNCTCC	6 cut(s)	1378, 1408, 3896, 3926, 4995, 5025
Bse118I	RCCGGY	2 cut(s)	1802, 2288
Bse3DI	GCAATG	5 cut(s)	1052, 1753, 3531, 4666, 4980
Bse8I	GATNNNNATC	1 cut(s)	1998
BseBI	CCWGG	5 cut(s)	2220, 3441, 4705, 5043, 5421
BseJI	GATNNNNATC	1 cut(s)	1998
BseMI	GCAATG	5 cut(s)	1052, 1753, 3531, 4666, 4980
BsePI	GCGCGC	2 cut(s)	85, 5377
BseRI	GAGGAG	5 cut(s)	656, 2387, 2719, 4671, 5117
BseSI	GKGCMC	6 cut(s)	448, 1231, 2722, 2782, 4517, 5407
BseX3I	CGGCCG	1 cut(s)	223
BseYI	CCCAGC	2 cut(s)	3433, 3551
BsgI	GTGCAG	1 cut(s)	4228
Bsh1236I	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
Bsh1285I	CGRYCG	2 cut(s)	226, 5478
BshFI	GGCC	9 cut(s)	32, 123, 225, 1695, 2872, 3814, 4708, 5250, 5631
BshNI	GGYRCC	2 cut(s)	473, 1226
BsiEI	CGRYCG	2 cut(s)	226, 5478
BsiHKAI	GWGCWC	8 cut(s)	656, 745, 925, 2153, 2722, 2782, 4060, 5407
BsiHKCI	CYCGRG	2 cut(s)	2874, 5342
BslFI	GGGAC	8 cut(s)	130, 1034, 2438, 3224, 4300, 4405, 4686, 5515
BsmAI	GTCTC	7 cut(s)	1203, 2346, 2796, 3372, 3697, 4268, 4276
BsmBI	CGTCTC	1 cut(s)	1203
BsmFI	GGGAC	8 cut(s)	130, 1034, 2438, 3224, 4300, 4405, 4686, 5515
BsmI	GAATGC	2 cut(s)	2290, 5467
BsnI	GGCC	9 cut(s)	32, 123, 225, 1695, 2872, 3814, 4708, 5250, 5631
Bso31I	GGTCTC	1 cut(s)	2346
BsoBI	CYCGRG	2 cut(s)	2874, 5342
Bsp119I	TTCGAA	1 cut(s)	2161
Bsp1720I	GCTNAGC	1 cut(s)	4896
Bsp19I	CCATGG	4 cut(s)	189, 1174, 3546, 4605
Bsp68I	TCGCGA	1 cut(s)	1599
BspANI	GGCC	9 cut(s)	32, 123, 225, 1695, 2872, 3814, 4708, 5250, 5631
BspFNI	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
BspHI	TCATGA	5 cut(s)	703, 1078, 4558, 5544, 5651
BspMAI	CTGCAG	2 cut(s)	1074, 4917
BspMI	ACCTGC	4 cut(s)	187, 498, 5247, 5541
BspOI	GCTAGC	2 cut(s)	1545, 2408
BspQI	GCTCTTC	2 cut(s)	2592, 4800
BspT104I	TTCGAA	1 cut(s)	2161
BspT107I	GGYRCC	2 cut(s)	473, 1226
BspTNI	GGTCTC	1 cut(s)	2346
BsrBI	CCGCTC	3 cut(s)	537, 917, 3173
BsrDI	GCAATG	5 cut(s)	1052, 1753, 3531, 4666, 4980
BsrFI	RCCGGY	2 cut(s)	1802, 2288
BssAI	RCCGGY	2 cut(s)	1802, 2288
BssHII	GCGCGC	2 cut(s)	85, 5377
BssNI	GRCGYC	1 cut(s)	474
BssSI	CACGAG	1 cut(s)	3412
BssT1I	CCWWGG	9 cut(s)	189, 343, 1174, 1893, 2505, 3546, 4605, 5586, 5632
Bst2BI	CACGAG	1 cut(s)	3412
Bst2UI	CCWGG	5 cut(s)	2220, 3441, 4705, 5043, 5421
Bst6I	CTCTTC	4 cut(s)	2592, 2853, 3339, 4800
BstACI	GRCGYC	1 cut(s)	474
BstAPI	GCANNNNNTGC	2 cut(s)	865, 5486
BstBI	TTCGAA	1 cut(s)	2161
BstDSI	CCRYGG	5 cut(s)	189, 1174, 3546, 4605, 4999
BstFNI	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
BstH2I	RGCGCY	2 cut(s)	477, 1548
BstMAI	GTCTC	7 cut(s)	1203, 2346, 2796, 3372, 3697, 4268, 4276
BstMCI	CGRYCG	2 cut(s)	226, 5478
BstNI	CCWGG	5 cut(s)	2220, 3441, 4705, 5043, 5421
BstNSI	RCATGY	6 cut(s)	1103, 2181, 3349, 4254, 4588, 5553
BstPAI	GACNNNNGTC	1 cut(s)	2387
BstSCI	CCNGG	9 cut(s)	140, 2218, 3439, 4703, 5041, 5068, 5174, 5419, 5504
BstSFI	CTRYAG	5 cut(s)	1070, 2853, 2946, 3399, 4913
BstSLI	GKGCMC	6 cut(s)	448, 1231, 2722, 2782, 4517, 5407
BstUI	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
BstV2I	GAAGAC	6 cut(s)	105, 902, 1569, 4211, 4325, 5144
BstX2I	RGATCY	7 cut(s)	1422, 1648, 1822, 2215, 3560, 3887, 5636
BstXI	CCANNNNNNTGG	2 cut(s)	1640, 4704
BstYI	RGATCY	7 cut(s)	1422, 1648, 1822, 2215, 3560, 3887, 5636
BstZI	CGGCCG	1 cut(s)	223
BsuI	GTATCC	3 cut(s)	2443, 3769, 5300
BsuRI	GGCC	9 cut(s)	32, 123, 225, 1695, 2872, 3814, 4708, 5250, 5631
BtgI	CCRYGG	5 cut(s)	189, 1174, 3546, 4605, 4999
BtgZI	GCGATG	2 cut(s)	390, 510
BtrI	CACGTC	2 cut(s)	1831, 4700
BtsI	GCAGTG	4 cut(s)	863, 939, 3754, 4216
BtuMI	TCGCGA	1 cut(s)	1599
BveI	ACCTGC	4 cut(s)	187, 498, 5247, 5541
CaiI	CAGNNNCTG	6 cut(s)	865, 1367, 1967, 2219, 2954, 3440
CciI	TCATGA	5 cut(s)	703, 1078, 4558, 5544, 5651
CciNI	GCGGCCGC	1 cut(s)	223
Cfr10I	RCCGGY	2 cut(s)	1802, 2288
Cfr13I	GGNCC	9 cut(s)	122, 144, 159, 193, 1517, 1694, 1930, 4526, 5502
CseI	GACGC	5 cut(s)	616, 1185, 2052, 2756, 5135
CsiI	ACCWGGT	1 cut(s)	5419
CspCI	CAANNNNNGTGG	4 cut(s)	2112, 2147, 5211, 5246
DinI	GGCGCC	1 cut(s)	475
DraI	TTTAAA	2 cut(s)	2194, 2262
DraIII	CACNNNGTG	1 cut(s)	5423
EaeI	YGGCCR	2 cut(s)	223, 5248
EagI	CGGCCG	1 cut(s)	223
Eam1104I	CTCTTC	4 cut(s)	2592, 2853, 3339, 4800
EarI	CTCTTC	4 cut(s)	2592, 2853, 3339, 4800
EciI	GGCGGA	2 cut(s)	41, 3397
Ecl136II	GAGCTC	2 cut(s)	2151, 4058
EclXI	CGGCCG	1 cut(s)	223
Eco130I	CCWWGG	9 cut(s)	189, 343, 1174, 1893, 2505, 3546, 4605, 5586, 5632
Eco24I	GRGCYC	3 cut(s)	2153, 4060, 5324
Eco31I	GGTCTC	1 cut(s)	2346
Eco32I	GATATC	2 cut(s)	3286, 5089
Eco47I	GGWCC	7 cut(s)	144, 159, 193, 1517, 1930, 4526, 5502
Eco47III	AGCGCT	1 cut(s)	1546
Eco52I	CGGCCG	1 cut(s)	223
Eco53kI	GAGCTC	2 cut(s)	2151, 4058
Eco57I	CTGAAG	9 cut(s)	417, 434, 566, 1149, 1726, 2085, 4161, 4319, 5585
Eco88I	CYCGRG	2 cut(s)	2874, 5342
EcoICRI	GAGCTC	2 cut(s)	2151, 4058
EcoO109I	RGGNCCY	2 cut(s)	1517, 4526
EcoRI	GAATTC	2 cut(s)	1341, 4597
EcoRII	CCWGG	5 cut(s)	2218, 3439, 4703, 5041, 5419
EcoRV	GATATC	2 cut(s)	3286, 5089
EcoT14I	CCWWGG	9 cut(s)	189, 343, 1174, 1893, 2505, 3546, 4605, 5586, 5632
EcoT22I	ATGCAT	1 cut(s)	2276
EcoT38I	GRGCYC	3 cut(s)	2153, 4060, 5324
EgeI	GGCGCC	1 cut(s)	475
EheI	GGCGCC	1 cut(s)	475
ErhI	CCWWGG	9 cut(s)	189, 343, 1174, 1893, 2505, 3546, 4605, 5586, 5632
Esp3I	CGTCTC	1 cut(s)	1203
FalI	AAGNNNNNCTT	8 cut(s)	1341, 1373, 2850, 2882, 4182, 4214, 4668, 4700
FaqI	GGGAC	8 cut(s)	130, 1034, 2438, 3224, 4300, 4405, 4686, 5515
FauI	CCCGC	6 cut(s)	15, 133, 155, 530, 910, 4325
FbaI	TGATCA	2 cut(s)	5284, 5299
FblI	GTMKAC	1 cut(s)	3163
FriOI	GRGCYC	3 cut(s)	2153, 4060, 5324
GsaI	CCCAGC	2 cut(s)	3437, 3555
GsuI	CTGGAG	6 cut(s)	984, 1242, 1616, 2137, 2955, 3675
HaeII	RGCGCY	2 cut(s)	477, 1548
HaeIII	GGCC	9 cut(s)	32, 123, 225, 1695, 2872, 3814, 4708, 5250, 5631
HgaI	GACGC	5 cut(s)	616, 1185, 2052, 2756, 5135
Hin1I	GRCGYC	1 cut(s)	474
HincII	GTYRAC	6 cut(s)	831, 1236, 3164, 4153, 4242, 5125
HindII	GTYRAC	6 cut(s)	831, 1236, 3164, 4153, 4242, 5125
HindIII	AAGCTT	6 cut(s)	1131, 1874, 2187, 2537, 4844, 5567
HpaI	GTTAAC	1 cut(s)	4153
HphI	GGTGA	9 cut(s)	168, 2109, 2900, 4393, 4517, 4676, 4976, 5353, 5366
Hpy99I	CGWCG	5 cut(s)	2046, 2822, 3003, 3165, 3168
HpyCH4IV	ACGT	4 cut(s)	656, 1830, 2817, 4699
HpySE526I	ACGT	4 cut(s)	656, 1830, 2817, 4699
Hsp92I	GRCGYC	1 cut(s)	474
KasI	GGCGCC	1 cut(s)	473
Ksp22I	TGATCA	2 cut(s)	5284, 5299
KspAI	GTTAAC	1 cut(s)	4153
LguI	GCTCTTC	2 cut(s)	2592, 4800
MabI	ACCWGGT	1 cut(s)	5419
MaeII	ACGT	4 cut(s)	656, 1830, 2817, 4699
MaeIII	GTNAC	9 cut(s)	861, 1183, 2051, 2239, 3416, 3930, 4232, 4664, 5395
MbiI	CCGCTC	3 cut(s)	537, 917, 3173
MfeI	CAATTG	2 cut(s)	630, 3917
MflI	RGATCY	7 cut(s)	1422, 1648, 1822, 2215, 3560, 3887, 5636
Mly113I	GGCGCC	1 cut(s)	474
MmeI	TCCRAC	5 cut(s)	3823, 3927, 3933, 4095, 5211
Mph1103I	ATGCAT	1 cut(s)	2276
MroXI	GAANNNNTTC	5 cut(s)	1375, 1480, 1579, 2862, 5467
MslI	CAYNNNNRTG	3 cut(s)	756, 798, 4647
MspA1I	CMGCKG	2 cut(s)	785, 1028
MspR9I	CCNGG	9 cut(s)	142, 2220, 3441, 4705, 5043, 5070, 5176, 5421, 5506
MunI	CAATTG	2 cut(s)	630, 3917
Mva1269I	GAATGC	2 cut(s)	2290, 5467
MvaI	CCWGG	5 cut(s)	2220, 3441, 4705, 5043, 5421
MvnI	CGCG	9 cut(s)	87, 150, 220, 222, 1599, 3013, 4320, 5129, 5379
NarI	GGCGCC	1 cut(s)	474
NciI	CCSGG	4 cut(s)	142, 5070, 5176, 5506
NcoI	CCATGG	4 cut(s)	189, 1174, 3546, 4605
NheI	GCTAGC	2 cut(s)	1541, 2404
NmeAIII	GCCGAG	1 cut(s)	5389
NmuCI	GTSAC	4 cut(s)	861, 3416, 3930, 4664
NotI	GCGGCCGC	1 cut(s)	223
NruI	TCGCGA	1 cut(s)	1599
NsiI	ATGCAT	1 cut(s)	2276
NspI	RCATGY	6 cut(s)	1103, 2181, 3349, 4254, 4588, 5553
NspV	TTCGAA	1 cut(s)	2161
PaeI	GCATGC	2 cut(s)	1103, 3349
PaeR7I	CTCGAG	2 cut(s)	2874, 5342
PagI	TCATGA	5 cut(s)	703, 1078, 4558, 5544, 5651
PaqCI	CACCTGC	1 cut(s)	187
PauI	GCGCGC	2 cut(s)	85, 5377
PciSI	GCTCTTC	2 cut(s)	2592, 4800
PcsI	WCGNNNNNNNCGW	6 cut(s)	70, 206, 653, 2029, 2881, 4420
PctI	GAATGC	2 cut(s)	2290, 5467
PdmI	GAANNNNTTC	5 cut(s)	1375, 1480, 1579, 2862, 5467
PflFI	GACNNNGTC	2 cut(s)	142, 4670
PflMI	CCANNNNNTGG	3 cut(s)	976, 3440, 5423
PluTI	GGCGCC	1 cut(s)	477
PpuMI	RGGWCCY	2 cut(s)	1517, 4526
PshAI	GACNNNNGTC	1 cut(s)	2387
PshBI	ATTAAT	1 cut(s)	1733
PsiI	TTATAA	1 cut(s)	4842
Psp124BI	GAGCTC	2 cut(s)	2153, 4060
Psp5II	RGGWCCY	2 cut(s)	1517, 4526
Psp6I	CCWGG	5 cut(s)	2218, 3439, 4703, 5041, 5419
PspFI	CCCAGC	2 cut(s)	3433, 3551
PspGI	CCWGG	5 cut(s)	2218, 3439, 4703, 5041, 5419
PspPI	GGNCC	9 cut(s)	122, 144, 159, 193, 1517, 1694, 1930, 4526, 5502
PspPPI	RGGWCCY	2 cut(s)	1517, 4526
PspXI	VCTCGAGB	1 cut(s)	2874
PsrI	GAACNNNNNNTAC	2 cut(s)	58, 90
PstI	CTGCAG	2 cut(s)	1074, 4917
PstNI	CAGNNNCTG	6 cut(s)	865, 1367, 1967, 2219, 2954, 3440
PsuI	RGATCY	7 cut(s)	1422, 1648, 1822, 2215, 3560, 3887, 5636
PsyI	GACNNNGTC	2 cut(s)	142, 4670
PteI	GCGCGC	2 cut(s)	85, 5377
PvuII	CAGCTG	1 cut(s)	1028
RruI	TCGCGA	1 cut(s)	1599
RseI	CAYNNNNRTG	3 cut(s)	756, 798, 4647
SacI	GAGCTC	2 cut(s)	2153, 4060
SalI	GTCGAC	1 cut(s)	3162
SapI	GCTCTTC	2 cut(s)	2592, 4800
Sau96I	GGNCC	9 cut(s)	122, 144, 159, 193, 1517, 1694, 1930, 4526, 5502
ScaI	AGTACT	3 cut(s)	1951, 2204, 2378
ScrFI	CCNGG	9 cut(s)	142, 2220, 3441, 4705, 5043, 5070, 5176, 5421, 5506
SexAI	ACCWGGT	1 cut(s)	5419
SfcI	CTRYAG	5 cut(s)	1070, 2853, 2946, 3399, 4913
SfoI	GGCGCC	1 cut(s)	475
Sfr274I	CTCGAG	2 cut(s)	2874, 5342
SfuI	TTCGAA	1 cut(s)	2161
SgrDI	CGTCGACG	1 cut(s)	3162
SinI	GGWCC	7 cut(s)	144, 159, 193, 1517, 1930, 4526, 5502
SlaI	CTCGAG	2 cut(s)	2874, 5342
SmiMI	CAYNNNNRTG	3 cut(s)	756, 798, 4647
SmlI	CTYRAG	6 cut(s)	875, 1188, 2874, 4111, 4535, 5342
SmoI	CTYRAG	6 cut(s)	875, 1188, 2874, 4111, 4535, 5342
SphI	GCATGC	2 cut(s)	1103, 3349
SspDI	GGCGCC	1 cut(s)	473
SspI	AATATT	5 cut(s)	1786, 2110, 4000, 4499, 5214
SstI	GAGCTC	2 cut(s)	2153, 4060
StyD4I	CCNGG	9 cut(s)	140, 2218, 3439, 4703, 5041, 5068, 5174, 5419, 5504
StyI	CCWWGG	9 cut(s)	189, 343, 1174, 1893, 2505, 3546, 4605, 5586, 5632
TaiI	ACGT	4 cut(s)	659, 1833, 2820, 4702
TauI	GCSGC	8 cut(s)	185, 225, 228, 231, 785, 3173, 3545, 4810
TseFI	GTSAC	4 cut(s)	861, 3416, 3930, 4664
Tsp45I	GTSAC	4 cut(s)	861, 3416, 3930, 4664
TspGWI	ACGGA	3 cut(s)	1479, 1587, 4988
Tth111I	GACNNNGTC	2 cut(s)	142, 4670
Van91I	CCANNNNNTGG	3 cut(s)	976, 3440, 5423
VneI	GTGCAC	3 cut(s)	2718, 2778, 5403
VpaK11BI	GGWCC	7 cut(s)	144, 159, 193, 1517, 1930, 4526, 5502
VspI	ATTAAT	1 cut(s)	1733
XapI	RAATTY	8 cut(s)	1341, 1371, 1957, 2071, 2980, 4145, 4185, 4597
XceI	RCATGY	6 cut(s)	1103, 2181, 3349, 4254, 4588, 5553
XcmI	CCANNNNNNNNNTGG	2 cut(s)	4682, 5396
XhoI	CTCGAG	2 cut(s)	2874, 5342
XmaJI	CCTAGG	1 cut(s)	343
XmiI	GTMKAC	1 cut(s)	3163
XmnI	GAANNNNTTC	5 cut(s)	1375, 1480, 1579, 2862, 5467
ZrmI	AGTACT	3 cut(s)	1951, 2204, 2378
Zsp2I	ATGCAT	1 cut(s)	2276

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.