Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003039

Physical Location & Seq

Genomic Coordinates Reverse (-)

23131465 .. 23135881

Gene Structure 4417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1117500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGGAGAAAGGCAAATCGAGCAAACGCGACCGCAGGGATCGAGACAGAGACTACGATAAGCATCGCAGCCGTGACGCCGATGAAAAGCGATCGTCAAAGGACACCGATTACCACCGCCACCACCATCGATCGGACCGCGATTCCAAGCGCGAGCCCCGCGACCACAAATCTCGTCGGGAACGGTCACGCGAGCCCAGTGAAGACGGCAGGGATGTCTCGCGGGACCACCGCGAGCGGTCCTACGAGCCCAGGGCCGAGAGGGAAGGGAGCAGAGAGAGGTCCAGGCATCGGGAAGCGAAACGCGAGAGGTCGGAGGAGCGAGAGGAATCGAAGCGTAAGAGGAAAGAGAGGGAGAGGAGTGAGGGTAGAGATGGCGATCGTGATGGTTTAGAAGATAAGAAGAGGTCCAGGGTTTCGGAGGGGGAGAGGAGAGAGAGGAAGCGATTCGAGGAAGATGGTCAGGAAGTGAATGGAGATCAGAGAAAGAGTAGGAGGTTTGATGACCCCCGAGTCAAGGAAGAGGATGGAGAGAATGGTGGAGCTGTTGGACGGAGAACTGAATTGAATGTGAATGCGAGTGAGGTTAATGTTAAGAAAGTAGAAGTTGGGGAGGATCAGATTGGCAATTTCCAGAGCGTCGCCGCCCCCCCAAATGGGAGTGGTTGGGTGGGTAATGGTGCTGCTCTGGAACCTGCTATGACATCAAGGACTATGCCTGAGACCTCTTTTGCTCCCAATCATCCCCTTCCTACCAAGGTATCTTCAATTCCTACAACAAATGAAAATAAGGGAGTTAGTATTACCAGATCTCATGAGGTTCATGGAAACTCTAGTACAGATGGAACATCTTCAACTGCTGGGAAAAGTGCGAATCTGTCTCTTGGTGATGTAGAAAAAATTAAGAGAACTTTACAAAAGCAGAAGGAATTGACTGAGAAGCTGAAGAAGCTTAACCAGTTGAAGAAGGGCGGCAATGCAAGCAAAGATGCTAGCCATAACTTGGAAACGAAGGAGGCACTGAAACCACCATCTACCACTGGTGGGATACTACATGCACCGGCTCCATCAACAGCTGGTGGTACAGCTGCTTCTGTGGCAACATTTGATTCATCTGCACTACCCATTTCTGCAGCAGCCATTCCCAATTTTGAAGCTGTAAAACGTGCACAAGAGCTTGCTGCTCAGATGGGGTTCCGTCATGACCCCGAGTTTTCTCCTCTTATAAACGTGTTTCCCGGACAGGTGGCAACTGATGTTGCTGCCCCTCAGAAGCCTACTAAGGCCCCTGTTCTTCGTCTTGATGCCCTTGGAAGGGAAATAGATGAGCACGGAAATTTAGTGAATGTGACCAAACCAAACAACCTCAGCACTCTTAAGGTCAACATTAACAAACAGAAGAAAGAAGCATTCCAGATTCTAAAACCTGAATTGGATGTGGATCCTGAAAAGAATCCTCATTTTGATCCGGATGTGGGCATCAGTAAAAAATTTTTGAGGCCAAAGAGGATGGGTTTTGCATTTGTAGAGGAAGGAAAATGGACAAAAGATGCTGAGCTTATAAAATTAAAGAGTAAATTTGGAGAAGCACAAGCCAAAGAGCAAAGAGCAAAGCAGCAACAGTTTGCAAAGGCAAGGGCAGCGCCAGATATTAATCCAAATTTGATAGAGGTAGGAGAGAGAGTTATCACCAAAGAAAAGCCAAAGGATCCAATTCCTGAAATCGAGTGGTGGGATGTGCCCCTCTTGCACTCTGGTACTTATAATGAAGTCATCGATGGTACAGTAGCTGAAGAAAAATTAAAGATGGAAAAAATCACAATCTATATTGAGCATCCTCAACCTATTGAGCCCCCTACTGAGCCAGCTCCTCCACCGCCTCAACCTCTGAAGTTGACCAAGAAGGAACAGAAAAAACTACGTACACAGAAACGTCTGGCCAGAGAGAAAGATAGGCAGGAGATGATTAGACAAGGCCTACTTGAGCCTCCAAAGCCAAAAGTTAAAATGAGTAATTTGATGAAAGTTCTTGGCTCTGAAGCAACCCAAGATCCAACCAGGCTTGAAAAGGAGATCCGGAGTGCAGCTGCTGAACGTGAACAAGCTCATGCAGACAGGAATATAGCACGCAAGCTCACTCCTGCCGAACGACGCGAGAAGAAAGAAAGGAAGCTTTTTGATGACCCAAATAACGTGGAGACCATAGTCTCAGTTTACAGGATCAATGAGCTGTCGCATCCAAAGGCCCGCTTCAAGATAGATGTTAATGCGCGAGAGAACCGGCTGACTGGATCTTGTGTGATATCAGATGGTATTAATGTTGCGGTTGTTGAAGGTGGAAGCAAGTCTATTAAGAGGTATGCTAAGGTTATGCTTAGGCGCATAAACTGGGCTGAAGCAGTAAAAGAAGAGGAAGAAGATGATGATGTTGAAGATGACAAGCCTCCTAACAAGTGCGTGTTAGTATGGCAAGGAAGTGTTGCCAGACCGAGCTTCAATAGATTTTATGTTCATGAATGCATGACCGAAGCTGCTGCCCGGAAGATTTTTGCTGATGCTGGCGTTGCTCATTACTGGGATCTTGCGGTCAACTTCCAAGATGATAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022414 GO:0030532 GO:0031323 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DUF1115 PRP3

Protein Analysis

869

Amino Acids

98.23

Weight (kDa)

9.3

Isoelectric Point (pI)

51.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRP3	PF08572	484 - 712	3.7e-64	pre-mRNA processing factor 3 domain
Prp3_C	PF06544	736 - 861	1.7e-41	Small nuclear ribonucleoprotein Prp3, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28060 AT1G28060 AT1G28060 AT3G55930
fragaria_vesca	FvH4_2g04410 FvH4_7g20820 FvH4_7g20820 FvH4_7g20820 FvH4_7g20820 FvH4_7g20820
malus_domestica	MD01G1117500.v1.1 MD07G1185000.v1.1
prunus_persica	Prupe.2G224900_v2.0.a1 Prupe.2G224900_v2.0.a1 Prupe.6G042200_v2.0.a1
pyrus_communis	pycom01g14520 pycom07g17720
rosa_chinensis	RchiOBHm_Chr1g0365041
rosa_laevigata	RLG00000027448
rosa_multiflora	Rmu_sc0002451.1_g000035
rosa_roxburghii	Rroxscaffold_4G00291520 Rroxscaffold_4G00291970
rosa_rugosa	Rorug01G0319100 Rorug01G0319200
rosa_samantha	Rh1AG188900 Rh1AG328200 Rh1BG289700 Rh1CG174500 Rh1CG306300 Rh1DG163200 Rh1DG321100 Rh4DG027000
rosa_wichuraiana	Rw1G029120 Rw2G017970 Rw3G016000 Rw4G013150 Rw5G015830 Rw5G032310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1223, 1559, 1761
AasI	GACNNNNNNGTC	1 cut(s)	509
Acc36I	ACCTGC	1 cut(s)	700
AccB7I	CCANNNNNTGG	1 cut(s)	1000
AccBSI	CCGCTC	1 cut(s)	235
AccIII	TCCGGA	2 cut(s)	1465, 2073
AcoI	YGGCCR	1 cut(s)	1935
AcsI	RAATTY	4 cut(s)	1333, 1487, 1574, 1657
AcuI	CTGAAG	5 cut(s)	962, 1809, 1907, 2055, 2412
AcyI	GRCGYC	1 cut(s)	75
AfaI	GTAC	5 cut(s)	835, 1081, 1756, 1780, 1921
AflII	CTTAAG	1 cut(s)	1373
AflIII	ACRYGT	1 cut(s)	1227
AjnI	CCWGG	4 cut(s)	248, 281, 407, 2054
Alw21I	GWGCWC	2 cut(s)	1168, 1329
Alw26I	GTCTC	7 cut(s)	36, 42, 220, 713, 882, 2189, 2209
Alw44I	GTGCAC	1 cut(s)	1164
AlwNI	CAGNNNCTG	2 cut(s)	1787, 2087
Ama87I	CYCGRG	2 cut(s)	507, 1205
Aor13HI	TCCGGA	2 cut(s)	1465, 2073
AoxI	GGCC	6 cut(s)	252, 1281, 1496, 1935, 1972, 2241
ApaLI	GTGCAC	1 cut(s)	1164
ApoI	RAATTY	4 cut(s)	1333, 1487, 1574, 1657
AseI	ATTAAT	2 cut(s)	1650, 2313
Asp700I	GAANNNNTTC	2 cut(s)	443, 847
AspLEI	GCGC	4 cut(s)	150, 1642, 2269, 2379
AspS9I	GGNCC	8 cut(s)	133, 223, 237, 252, 279, 405, 1282, 2242
AsuC2I	CCSGG	2 cut(s)	1236, 2536
AsuHPI	GGTGA	2 cut(s)	896, 1678
AsuNHI	GCTAGC	1 cut(s)	989
AvaI	CYCGRG	2 cut(s)	507, 1205
AvaII	GGWCC	5 cut(s)	133, 223, 237, 279, 405
BaeGI	GKGCMC	2 cut(s)	1168, 1740
BaeI	ACNNNNGTAYC	2 cut(s)	741, 774
BalI	TGGCCA	1 cut(s)	1937
BamHI	GGATCC	2 cut(s)	1438, 1705
BanII	GRGCYC	4 cut(s)	156, 195, 249, 1851
BarI	GAAGNNNNNNTAC	2 cut(s)	1072, 1104
BbsI	GAAGAC	1 cut(s)	207
Bbv12I	GWGCWC	2 cut(s)	1168, 1329
BbvCI	CCTCAGC	1 cut(s)	1364
BceAI	ACGGC	2 cut(s)	54, 220
BcgI	CGANNNNNNTGC	2 cut(s)	2513, 2547
BciT130I	CCWGG	4 cut(s)	250, 283, 409, 2056
BciVI	GTATCC	1 cut(s)	1038
BcnI	CCSGG	2 cut(s)	1236, 2536
BcoDI	GTCTC	7 cut(s)	36, 42, 220, 713, 882, 2189, 2209
BfaI	CTAG	2 cut(s)	831, 990
BfmI	CTRYAG	1 cut(s)	1128
BfoI	RGCGCY	1 cut(s)	1643
BfrI	CTTAAG	1 cut(s)	1373
BfuAI	ACCTGC	1 cut(s)	700
BfuI	GTATCC	1 cut(s)	1038
BglII	AGATCT	1 cut(s)	806
BlpI	GCTNAGC	1 cut(s)	1551
Bme1390I	CCNGG	6 cut(s)	250, 283, 409, 1236, 2056, 2536
Bme18I	GGWCC	5 cut(s)	133, 223, 237, 279, 405
BmeT110I	CYCGRG	2 cut(s)	507, 1205
BmgT120I	GGNCC	8 cut(s)	133, 223, 237, 252, 279, 405, 1282, 2242
BmiI	GGNNCC	7 cut(s)	224, 690, 1062, 1193, 1284, 1440, 1707
BmrFI	CCNGG	6 cut(s)	250, 283, 409, 1236, 2056, 2536
BmrI	ACTGGG	3 cut(s)	189, 2395, 2581
BmsI	GCATC	9 cut(s)	70, 295, 976, 1291, 1485, 1537, 1840, 2242, 2542
BmtI	GCTAGC	1 cut(s)	993
BmuI	ACTGGG	3 cut(s)	189, 2395, 2581
BoxI	GACNNNNGTC	1 cut(s)	2201
BpiI	GAAGAC	1 cut(s)	207
Bpu10I	CCTNAGC	3 cut(s)	1364, 2361, 2372
Bpu1102I	GCTNAGC	1 cut(s)	1551
BpuEI	CTTGAG	1 cut(s)	2000
BpuMI	CCSGG	2 cut(s)	1236, 2536
Bsa29I	ATCGAT	2 cut(s)	127, 1773
BsaAI	YACGTR	1 cut(s)	1919
BsaBI	GATNNNNATC	4 cut(s)	60, 375, 1437, 1650
BsaHI	GRCGYC	1 cut(s)	75
BsaI	GGTCTC	2 cut(s)	713, 2189
BsaJI	CCNNGG	5 cut(s)	248, 249, 408, 753, 1306
BsaWI	WCCGGW	2 cut(s)	1465, 2073
BsaXI	ACNNNNNCTCC	4 cut(s)	349, 379, 1665, 1695
Bse118I	RCCGGY	2 cut(s)	1057, 2277
Bse1I	ACTGG	6 cut(s)	195, 955, 1042, 2290, 2390, 2576
Bse3DI	GCAATG	1 cut(s)	979
Bse8I	GATNNNNATC	4 cut(s)	60, 375, 1437, 1650
BseAI	TCCGGA	2 cut(s)	1465, 2073
BseBI	CCWGG	4 cut(s)	250, 283, 409, 2056
BseCI	ATCGAT	2 cut(s)	127, 1773
BseDI	CCNNGG	5 cut(s)	248, 249, 408, 753, 1306
BseGI	GGATG	9 cut(s)	217, 529, 739, 1438, 1474, 1512, 1738, 1831, 2233
BseJI	GATNNNNATC	4 cut(s)	60, 375, 1437, 1650
BseMI	GCAATG	1 cut(s)	979
BseMII	CTCAG	8 cut(s)	708, 924, 1196, 1280, 1378, 1542, 1848, 2220
BseNI	ACTGG	6 cut(s)	195, 955, 1042, 2290, 2390, 2576
BseRI	GAGGAG	5 cut(s)	329, 370, 442, 1206, 1857
BseSI	GKGCMC	2 cut(s)	1168, 1740
BseYI	CCCAGC	1 cut(s)	857
BsgI	GTGCAG	2 cut(s)	1098, 2100
Bsh1285I	CGRYCG	4 cut(s)	31, 92, 131, 379
BshFI	GGCC	6 cut(s)	254, 1283, 1498, 1937, 1974, 2243
BshVI	ATCGAT	2 cut(s)	127, 1773
BsiEI	CGRYCG	4 cut(s)	31, 92, 131, 379
BsiHKAI	GWGCWC	2 cut(s)	1168, 1329
BsiHKCI	CYCGRG	2 cut(s)	507, 1205
BsiSI	CCGG	6 cut(s)	1058, 1236, 1466, 2074, 2278, 2536
BslFI	GGGAC	1 cut(s)	236
BsmAI	GTCTC	7 cut(s)	36, 42, 220, 713, 882, 2189, 2209
BsmFI	GGGAC	1 cut(s)	236
BsmI	GAATGC	3 cut(s)	577, 1406, 2519
BsnI	GGCC	6 cut(s)	254, 1283, 1498, 1937, 1974, 2243
Bso31I	GGTCTC	2 cut(s)	713, 2189
BsoBI	CYCGRG	2 cut(s)	507, 1205
Bsp1286I	GDGCHC	7 cut(s)	156, 195, 249, 1168, 1329, 1740, 1851
Bsp13I	TCCGGA	2 cut(s)	1465, 2073
Bsp1720I	GCTNAGC	1 cut(s)	1551
BspANI	GGCC	6 cut(s)	254, 1283, 1498, 1937, 1974, 2243
BspCNI	CTCAG	8 cut(s)	709, 925, 1195, 1279, 1377, 1543, 1849, 2219
BspDI	ATCGAT	2 cut(s)	127, 1773
BspEI	TCCGGA	2 cut(s)	1465, 2073
BspHI	TCATGA	3 cut(s)	811, 1198, 2509
BspLI	GGNNCC	7 cut(s)	224, 690, 1062, 1193, 1284, 1440, 1707
BspMAI	CTGCAG	1 cut(s)	1132
BspMI	ACCTGC	1 cut(s)	700
BspOI	GCTAGC	1 cut(s)	993
BspTI	CTTAAG	1 cut(s)	1373
BspTNI	GGTCTC	2 cut(s)	713, 2189
BsrBI	CCGCTC	1 cut(s)	235
BsrDI	GCAATG	1 cut(s)	979
BsrFI	RCCGGY	2 cut(s)	1057, 2277
BsrI	ACTGG	6 cut(s)	195, 955, 1042, 2290, 2390, 2576
BssAI	RCCGGY	2 cut(s)	1057, 2277
BssECI	CCNNGG	5 cut(s)	248, 249, 408, 753, 1306
BssNI	GRCGYC	1 cut(s)	75
BssT1I	CCWWGG	2 cut(s)	753, 1306
Bst2UI	CCWGG	4 cut(s)	250, 283, 409, 2056
Bst4CI	ACNGT	3 cut(s)	183, 1620, 1783
Bst6I	CTCTTC	3 cut(s)	395, 513, 2400
BstACI	GRCGYC	1 cut(s)	75
BstAFI	CTTAAG	1 cut(s)	1373
BstBAI	YACGTR	1 cut(s)	1919
BstF5I	GGATG	9 cut(s)	217, 529, 739, 1438, 1474, 1512, 1738, 1831, 2233
BstH2I	RGCGCY	1 cut(s)	1643
BstHHI	GCGC	4 cut(s)	150, 1642, 2269, 2379
BstMAI	GTCTC	7 cut(s)	36, 42, 220, 713, 882, 2189, 2209
BstMCI	CGRYCG	4 cut(s)	31, 92, 131, 379
BstMWI	GCNNNNNNNGC	9 cut(s)	18, 156, 946, 978, 1094, 1637, 1744, 1990, 2561
BstNI	CCWGG	4 cut(s)	250, 283, 409, 2056
BstNSI	RCATGY	1 cut(s)	1055
BstPAI	GACNNNNGTC	1 cut(s)	2201
BstSCI	CCNGG	6 cut(s)	248, 281, 407, 1234, 2054, 2534
BstSFI	CTRYAG	1 cut(s)	1128
BstSLI	GKGCMC	2 cut(s)	1168, 1740
BstSNI	TACGTA	1 cut(s)	1919
BstV2I	GAAGAC	1 cut(s)	207
BstX2I	RGATCY	7 cut(s)	806, 1438, 1705, 2047, 2070, 2288, 2575
BstYI	RGATCY	7 cut(s)	806, 1438, 1705, 2047, 2070, 2288, 2575
Bsu15I	ATCGAT	2 cut(s)	127, 1773
BsuI	GTATCC	1 cut(s)	1038
BsuRI	GGCC	6 cut(s)	254, 1283, 1498, 1937, 1974, 2243
BsuTUI	ATCGAT	2 cut(s)	127, 1773
BtgZI	GCGATG	1 cut(s)	47
BtsCI	GGATG	9 cut(s)	217, 529, 739, 1438, 1474, 1512, 1738, 1831, 2233
BtsIMutI	CAGTG	3 cut(s)	202, 1016, 1035
BveI	ACCTGC	1 cut(s)	700
CaiI	CAGNNNCTG	2 cut(s)	1787, 2087
CciI	TCATGA	3 cut(s)	811, 1198, 2509
CfoI	GCGC	4 cut(s)	150, 1642, 2269, 2379
Cfr10I	RCCGGY	2 cut(s)	1057, 2277
Cfr13I	GGNCC	8 cut(s)	133, 223, 237, 252, 279, 405, 1282, 2242
ClaI	ATCGAT	2 cut(s)	127, 1773
CpoI	CGGWCCG	1 cut(s)	133
CseI	GACGC	3 cut(s)	83, 625, 2157
Csp6I	GTAC	5 cut(s)	834, 1080, 1755, 1779, 1920
CspCI	CAANNNNNGTGG	2 cut(s)	2172, 2207
CspI	CGGWCCG	1 cut(s)	133
CviAII	CATG	7 cut(s)	812, 821, 1052, 1199, 2105, 2510, 2518
CviQI	GTAC	5 cut(s)	834, 1080, 1755, 1779, 1920
DrdI	GACNNNNNNGTC	1 cut(s)	509
DseDI	GACNNNNNNGTC	1 cut(s)	509
EaeI	YGGCCR	1 cut(s)	1935
Eam1104I	CTCTTC	3 cut(s)	395, 513, 2400
EarI	CTCTTC	3 cut(s)	395, 513, 2400
Eco105I	TACGTA	1 cut(s)	1919
Eco130I	CCWWGG	2 cut(s)	753, 1306
Eco147I	AGGCCT	1 cut(s)	1974
Eco24I	GRGCYC	4 cut(s)	156, 195, 249, 1851
Eco31I	GGTCTC	2 cut(s)	713, 2189
Eco32I	GATATC	1 cut(s)	2301
Eco47I	GGWCC	5 cut(s)	133, 223, 237, 279, 405
Eco57I	CTGAAG	5 cut(s)	962, 1809, 1907, 2055, 2412
Eco88I	CYCGRG	2 cut(s)	507, 1205
EcoO109I	RGGNCCY	1 cut(s)	1282
EcoRII	CCWGG	4 cut(s)	248, 281, 407, 2054
EcoRV	GATATC	1 cut(s)	2301
EcoT14I	CCWWGG	2 cut(s)	753, 1306
EcoT22I	ATGCAT	1 cut(s)	2519
EcoT38I	GRGCYC	4 cut(s)	156, 195, 249, 1851
ErhI	CCWWGG	2 cut(s)	753, 1306
FaeI	CATG	7 cut(s)	815, 824, 1055, 1202, 2108, 2513, 2521
FalI	AAGNNNNNCTT	4 cut(s)	1962, 1994, 2231, 2263
FaqI	GGGAC	1 cut(s)	236
FatI	CATG	7 cut(s)	811, 820, 1051, 1198, 2104, 2509, 2517
FauI	CCCGC	3 cut(s)	164, 213, 2252
FokI	GGATG	9 cut(s)	224, 536, 726, 1445, 1481, 1519, 1745, 1818, 2220
FriOI	GRGCYC	4 cut(s)	156, 195, 249, 1851
FspBI	CTAG	2 cut(s)	831, 990
GlaI	GCGC	4 cut(s)	149, 1641, 2268, 2378
GsaI	CCCAGC	1 cut(s)	861
HaeII	RGCGCY	1 cut(s)	1643
HaeIII	GGCC	6 cut(s)	254, 1283, 1498, 1937, 1974, 2243
HapII	CCGG	6 cut(s)	1058, 1236, 1466, 2074, 2278, 2536
HgaI	GACGC	3 cut(s)	83, 625, 2157
HhaI	GCGC	4 cut(s)	150, 1642, 2269, 2379
Hin1I	GRCGYC	1 cut(s)	75
Hin1II	CATG	7 cut(s)	815, 824, 1055, 1202, 2108, 2513, 2521
Hin6I	GCGC	4 cut(s)	148, 1640, 2267, 2377
HinP1I	GCGC	4 cut(s)	148, 1640, 2267, 2377
HincII	GTYRAC	3 cut(s)	1381, 1893, 2587
HindII	GTYRAC	3 cut(s)	1381, 1893, 2587
HindIII	AAGCTT	2 cut(s)	947, 2168
HinfI	GANTC	8 cut(s)	140, 326, 444, 510, 871, 1106, 1414, 1450
HpaII	CCGG	6 cut(s)	1058, 1236, 1466, 2074, 2278, 2536
HphI	GGTGA	2 cut(s)	896, 1678
Hpy166II	GTNNAC	7 cut(s)	1166, 1381, 1893, 1922, 2096, 2212, 2587
Hpy8I	GTNNAC	7 cut(s)	1166, 1381, 1893, 1922, 2096, 2212, 2587
Hpy99I	CGWCG	3 cut(s)	177, 641, 2151
HpyAV	CCTTC	9 cut(s)	257, 755, 916, 958, 1003, 1305, 1523, 1894, 2324
HpyCH4III	ACNGT	3 cut(s)	183, 1620, 1783
HpyCH4IV	ACGT	6 cut(s)	1162, 1227, 1918, 1930, 2092, 2190
HpyF10VI	GCNNNNNNNGC	9 cut(s)	18, 156, 946, 978, 1094, 1637, 1744, 1990, 2561
HpySE526I	ACGT	6 cut(s)	1162, 1227, 1918, 1930, 2092, 2190
Hsp92I	GRCGYC	1 cut(s)	75
Hsp92II	CATG	7 cut(s)	815, 824, 1055, 1202, 2108, 2513, 2521
HspAI	GCGC	4 cut(s)	148, 1640, 2267, 2377
Kpn2I	TCCGGA	2 cut(s)	1465, 2073
LmnI	GCTCC	6 cut(s)	267, 316, 539, 736, 1066, 1870
LweI	GCATC	9 cut(s)	70, 295, 976, 1291, 1485, 1537, 1840, 2242, 2542
MaeI	CTAG	2 cut(s)	831, 990
MaeII	ACGT	6 cut(s)	1162, 1227, 1918, 1930, 2092, 2190
MaeIII	GTNAC	3 cut(s)	71, 183, 1345
MbiI	CCGCTC	1 cut(s)	235
MflI	RGATCY	7 cut(s)	806, 1438, 1705, 2047, 2070, 2288, 2575
MhlI	GDGCHC	7 cut(s)	156, 195, 249, 1168, 1329, 1740, 1851
MlsI	TGGCCA	1 cut(s)	1937
MluNI	TGGCCA	1 cut(s)	1937
MlyI	GAGTC	1 cut(s)	519
MmeI	TCCRAC	3 cut(s)	291, 526, 2075
Mox20I	TGGCCA	1 cut(s)	1937
Mph1103I	ATGCAT	1 cut(s)	2519
MroI	TCCGGA	2 cut(s)	1465, 2073
MroXI	GAANNNNTTC	2 cut(s)	443, 847
MscI	TGGCCA	1 cut(s)	1937
Msp20I	TGGCCA	1 cut(s)	1937
MspA1I	CMGCKG	3 cut(s)	1073, 1085, 2084
MspCI	CTTAAG	1 cut(s)	1373
MspI	CCGG	6 cut(s)	1058, 1236, 1466, 2074, 2278, 2536
MspR9I	CCNGG	6 cut(s)	250, 283, 409, 1236, 2056, 2536
Mva1269I	GAATGC	3 cut(s)	577, 1406, 2519
MvaI	CCWGG	4 cut(s)	250, 283, 409, 2056
MwoI	GCNNNNNNNGC	9 cut(s)	18, 156, 946, 978, 1094, 1637, 1744, 1990, 2561
NciI	CCSGG	2 cut(s)	1236, 2536
NheI	GCTAGC	1 cut(s)	989
NlaIII	CATG	7 cut(s)	815, 824, 1055, 1202, 2108, 2513, 2521
NlaIV	GGNNCC	7 cut(s)	224, 690, 1062, 1193, 1284, 1440, 1707
NmeAIII	GCCGAG	1 cut(s)	280
NmuCI	GTSAC	3 cut(s)	71, 183, 1345
NsiI	ATGCAT	1 cut(s)	2519
NspI	RCATGY	1 cut(s)	1055
PagI	TCATGA	3 cut(s)	811, 1198, 2509
PasI	CCCWGGG	1 cut(s)	249
PceI	AGGCCT	1 cut(s)	1974
PctI	GAATGC	3 cut(s)	577, 1406, 2519
PdmI	GAANNNNTTC	2 cut(s)	443, 847
PfeI	GAWTC	7 cut(s)	140, 326, 444, 871, 1106, 1414, 1450
PflMI	CCANNNNNTGG	1 cut(s)	1000
PfoI	TCCNGGA	1 cut(s)	1234
Ple19I	CGATCG	3 cut(s)	92, 131, 379
PleI	GAGTC	1 cut(s)	518
PpsI	GAGTC	1 cut(s)	518
Ppu21I	YACGTR	1 cut(s)	1919
PshAI	GACNNNNGTC	1 cut(s)	2201
PshBI	ATTAAT	2 cut(s)	1650, 2313
PsiI	TTATAA	3 cut(s)	1223, 1559, 1761
Psp6I	CCWGG	4 cut(s)	248, 281, 407, 2054
PspFI	CCCAGC	1 cut(s)	857
PspGI	CCWGG	4 cut(s)	248, 281, 407, 2054
PspN4I	GGNNCC	7 cut(s)	224, 690, 1062, 1193, 1284, 1440, 1707
PspPI	GGNCC	8 cut(s)	133, 223, 237, 252, 279, 405, 1282, 2242
PstI	CTGCAG	1 cut(s)	1132
PstNI	CAGNNNCTG	2 cut(s)	1787, 2087
PsuI	RGATCY	7 cut(s)	806, 1438, 1705, 2047, 2070, 2288, 2575
PvuI	CGATCG	3 cut(s)	92, 131, 379
PvuII	CAGCTG	3 cut(s)	1073, 1085, 2084
RsaI	GTAC	5 cut(s)	835, 1081, 1756, 1780, 1921
RsaNI	GTAC	5 cut(s)	834, 1080, 1755, 1779, 1920
Rsr2I	CGGWCCG	1 cut(s)	133
RsrII	CGGWCCG	1 cut(s)	133
Sau96I	GGNCC	8 cut(s)	133, 223, 237, 252, 279, 405, 1282, 2242
SchI	GAGTC	1 cut(s)	519
ScrFI	CCNGG	6 cut(s)	250, 283, 409, 1236, 2056, 2536
SduI	GDGCHC	7 cut(s)	156, 195, 249, 1168, 1329, 1740, 1851
SfaNI	GCATC	9 cut(s)	70, 295, 976, 1291, 1485, 1537, 1840, 2242, 2542
SfcI	CTRYAG	1 cut(s)	1128
SinI	GGWCC	5 cut(s)	133, 223, 237, 279, 405
SmlI	CTYRAG	2 cut(s)	1373, 1979
SmoI	CTYRAG	2 cut(s)	1373, 1979
SnaBI	TACGTA	1 cut(s)	1919
SseBI	AGGCCT	1 cut(s)	1974
SspMI	CTAG	2 cut(s)	831, 990
StuI	AGGCCT	1 cut(s)	1974
StyD4I	CCNGG	6 cut(s)	248, 281, 407, 1234, 2054, 2534
StyI	CCWWGG	2 cut(s)	753, 1306
TaaI	ACNGT	3 cut(s)	183, 1620, 1783
TaiI	ACGT	6 cut(s)	1165, 1230, 1921, 1933, 2095, 2193
TaqI	TCGA	7 cut(s)	17, 40, 127, 329, 447, 1722, 1773
TaqII	GACCGA	2 cut(s)	2500, 2537
TatI	WGTACW	1 cut(s)	833
TauI	GCSGC	2 cut(s)	644, 972
TfiI	GAWTC	7 cut(s)	140, 326, 444, 871, 1106, 1414, 1450
TscAI	CASTG	3 cut(s)	202, 1023, 1042
TseFI	GTSAC	3 cut(s)	71, 183, 1345
Tsp45I	GTSAC	3 cut(s)	71, 183, 1345
TspDTI	ATGAA	8 cut(s)	96, 795, 809, 1098, 1779, 2033, 2498, 2526
TspGWI	ACGGA	3 cut(s)	565, 1184, 1344
TspRI	CASTG	3 cut(s)	202, 1023, 1042
Van91I	CCANNNNNTGG	1 cut(s)	1000
Vha464I	CTTAAG	1 cut(s)	1373
VneI	GTGCAC	1 cut(s)	1164
VpaK11BI	GGWCC	5 cut(s)	133, 223, 237, 279, 405
VspI	ATTAAT	2 cut(s)	1650, 2313
XapI	RAATTY	4 cut(s)	1333, 1487, 1574, 1657
XceI	RCATGY	1 cut(s)	1055
XmnI	GAANNNNTTC	2 cut(s)	443, 847
XspI	CTAG	2 cut(s)	831, 990
Zsp2I	ATGCAT	1 cut(s)	2519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.