MD01G1157900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002494
Physical Location & Seq
Forward (+)
26469945 .. 26470836
892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1157900.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCCGGAACTTCTCCGTGCGCTTCCTGCAAGTTGCTGCGACGCCGATGCGCCAAAGACTGCATTTTTGCTCCTTTCTTTCCTTCAGATGACCCCCACAAGTTTGCCAATGTTCACAAGATCTTTGGTGCCAGCAATGTTAGCAAAATGTTGCAGGAGCTTCCGGTTCATCAGAGAGCAGATGCAGTGAGCAGTTTAGTGTATGAAGCAAATGCAAGAATGAGGGACCCAGTGTACGGATGCGTTGGGGCCATCTCTTACCTGCAAAACCAAGTCTCCGAGCTACAAATGCAGCTTGCTGTGGCTCAAGCTGAGATCCTTTGCATCAAAATGCAGCATGAGCCCATGGTCCCTCCACAACAGATAATGGACGGGCCAGATGATCAGAAATCGTATTTTCTCCACAACAACAATCTCGCTCAGTACCTCAGTTTTCCCCCTTCCAGCAATGTAATCCATGACTCTCTCAATAGGGAGAACATTTTCGGACACGACATGGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

169

Amino Acids

18.78

Weight (kDa)

6.36

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 103 1.7e-42 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 128
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 43
AfaI GTAC 2 cut(s) 236, 425
AfiI CCNNNNNNNGG 1 cut(s) 236
AluBI AGCT 4 cut(s) 160, 283, 295, 311
AluI AGCT 4 cut(s) 160, 283, 295, 311
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 310
AoxI GGCC 3 cut(s) 3, 249, 374
ApeKI GCWGC 3 cut(s) 37, 292, 334
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 2 cut(s) 23, 53
AspS9I GGNCC 4 cut(s) 226, 249, 349, 374
AvaII GGWCC 2 cut(s) 226, 349
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 24, 304, 346
BccI CCATC 1 cut(s) 260
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 280
BfuAI ACCTGC 1 cut(s) 270
BglII AGATCT 1 cut(s) 120
BisI GCNGC 3 cut(s) 38, 293, 335
BlsI GCNGC 3 cut(s) 39, 294, 336
Bme18I GGWCC 2 cut(s) 226, 349
BmgT120I GGNCC 4 cut(s) 226, 249, 349, 374
BmiI GGNNCC 5 cut(s) 130, 227, 228, 250, 351
BmrI ACTGGG 1 cut(s) 224
BmsI GCATC 4 cut(s) 38, 172, 230, 333
BmuI ACTGGG 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 230
Bse3DI GCAATG 2 cut(s) 142, 454
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 2 cut(s) 142, 454
BseMII CTCAG 3 cut(s) 303, 434, 442
BseNI ACTGG 1 cut(s) 230
BseXI GCAGC 3 cut(s) 24, 304, 346
BshFI GGCC 3 cut(s) 5, 251, 376
BshNI GGYRCC 1 cut(s) 128
BsiSI CCGG 2 cut(s) 6, 164
BslFI GGGAC 2 cut(s) 239, 335
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 2 cut(s) 239, 335
BsnI GGCC 3 cut(s) 5, 251, 376
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 3 cut(s) 120, 315, 382
Bsp19I CCATGG 1 cut(s) 345
BspANI GGCC 3 cut(s) 5, 251, 376
BspCNI CTCAG 3 cut(s) 304, 433, 441
BspLI GGNNCC 5 cut(s) 130, 227, 228, 250, 351
BspMI ACCTGC 1 cut(s) 270
BspPI GGATC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 142, 454
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 3 cut(s) 120, 315, 382
BssNI GRCGYC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 345
BstACI GRCGYC 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 133, 297
BstDEI CTNAG 3 cut(s) 312, 420, 428
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 245
BstHHI GCGC 2 cut(s) 23, 53
BstKTI GATC 3 cut(s) 123, 318, 385
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 120, 315, 382
BstMWI GCNNNNNNNGC 4 cut(s) 27, 141, 289, 340
BstV1I GCAGC 3 cut(s) 24, 304, 346
BstX2I RGATCY 2 cut(s) 120, 315
BstYI RGATCY 2 cut(s) 120, 315
BsuRI GGCC 3 cut(s) 5, 251, 376
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 1 cut(s) 245
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 192, 237
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 133, 297
CfoI GCGC 2 cut(s) 23, 53
Cfr13I GGNCC 4 cut(s) 226, 249, 349, 374
CseI GACGC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 235, 424
CviAII CATG 4 cut(s) 338, 346, 458, 496
CviJI RGCY 9 cut(s) 5, 160, 251, 283, 295, 305, 311, 343, 376
CviKI_1 RGCY 9 cut(s) 5, 160, 251, 283, 295, 305, 311, 343, 376
CviQI GTAC 2 cut(s) 235, 424
DdeI CTNAG 3 cut(s) 312, 420, 428
DpnI GATC 3 cut(s) 122, 317, 384
DpnII GATC 3 cut(s) 120, 315, 382
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 226, 349
Eco57I CTGAAG 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 4 cut(s) 341, 349, 461, 499
FaiI YATR 5 cut(s) 204, 339, 347, 459, 497
FaqI GGGAC 2 cut(s) 239, 335
FatI CATG 4 cut(s) 337, 345, 457, 495
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 38, 293, 335
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 3 cut(s) 38, 293, 335
GlaI GCGC 2 cut(s) 22, 52
GluI GCNGC 3 cut(s) 38, 293, 335
HaeIII GGCC 3 cut(s) 5, 251, 376
HapII CCGG 2 cut(s) 6, 164
HgaI GACGC 1 cut(s) 51
HhaI GCGC 2 cut(s) 23, 53
Hin1I GRCGYC 1 cut(s) 43
Hin1II CATG 4 cut(s) 341, 349, 461, 499
Hin6I GCGC 2 cut(s) 21, 51
HinP1I GCGC 2 cut(s) 21, 51
HinfI GANTC 1 cut(s) 461
HpaII CCGG 2 cut(s) 6, 164
Hpy166II GTNNAC 2 cut(s) 115, 235
Hpy188I TCNGA 5 cut(s) 88, 174, 280, 387, 488
Hpy8I GTNNAC 2 cut(s) 115, 235
Hpy99I CGWCG 1 cut(s) 45
HpyAV CCTTC 2 cut(s) 93, 450
HpyCH4V TGCA 9 cut(s) 30, 63, 154, 185, 215, 265, 292, 324, 334
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 141, 289, 340
HpyF3I CTNAG 3 cut(s) 312, 420, 428
Hsp92I GRCGYC 1 cut(s) 43
Hsp92II CATG 4 cut(s) 341, 349, 461, 499
HspAI GCGC 2 cut(s) 21, 51
KflI GGGWCCC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 120, 315, 382
LmnI GCTCC 2 cut(s) 76, 157
LpnPI CCDG 9 cut(s) 19, 40, 140, 145, 177, 243, 275, 390, 457
Lsp1109I GCAGC 3 cut(s) 24, 304, 346
LweI GCATC 4 cut(s) 38, 172, 230, 333
MalI GATC 3 cut(s) 122, 317, 384
MboI GATC 3 cut(s) 120, 315, 382
MflI RGATCY 2 cut(s) 120, 315
MhlI GDGCHC 1 cut(s) 345
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 3 cut(s) 216, 363, 437
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 1 cut(s) 505
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 2 cut(s) 6, 164
MwoI GCNNNNNNNGC 4 cut(s) 27, 141, 289, 340
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 120, 315, 382
NlaIII CATG 4 cut(s) 341, 349, 461, 499
NlaIV GGNNCC 5 cut(s) 130, 227, 228, 250, 351
PdmI GAANNNNTTC 1 cut(s) 482
PkrI GCNGC 3 cut(s) 39, 294, 336
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
PspN4I GGNNCC 5 cut(s) 130, 227, 228, 250, 351
PspPI GGNCC 4 cut(s) 226, 249, 349, 374
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 2 cut(s) 120, 315
RsaI GTAC 2 cut(s) 236, 425
RsaNI GTAC 2 cut(s) 235, 424
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 505
SatI GCNGC 3 cut(s) 38, 293, 335
Sau3AI GATC 3 cut(s) 120, 315, 382
Sau96I GGNCC 4 cut(s) 226, 249, 349, 374
SchI GAGTC 1 cut(s) 455
SduI GDGCHC 1 cut(s) 345
SetI ASST 6 cut(s) 162, 264, 285, 297, 313, 429
SfaNI GCATC 4 cut(s) 38, 172, 230, 333
SinI GGWCC 2 cut(s) 226, 349
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
StyI CCWWGG 1 cut(s) 345
Tru1I TTAA 1 cut(s) 505
Tru9I TTAA 1 cut(s) 505
TscAI CASTG 2 cut(s) 192, 237
TseI GCWGC 3 cut(s) 37, 292, 334
TspDTI ATGAA 2 cut(s) 158, 219
TspGWI ACGGA 2 cut(s) 6, 252
TspRI CASTG 2 cut(s) 192, 237
VpaK11BI GGWCC 2 cut(s) 226, 349
XmnI GAANNNNTTC 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.