MD07G1226600.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002494
Physical Location & Seq
Forward (+)
30226202 .. 30227101
900 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1226600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGATTATCTTTTTTCTAAGACACTGTAGTTTGATCTATATTAGGCACTTAAAGCCCCACACGTTCTCTTGTATTATAAATAGAGACTCCCCCCCATTTCATTTTCTCCAAGTTGTTTTCAATCACACTTTCTTCCTCTTCTCTCCACTACTCTCCAAGCTTTTCGAAATCAACATGGCCGGAACTTCGCCGTGCGCTTCCTGCAAGTTGCTGCGACGCCGATGCGCCAAAGACTGCATTTTTGCTCCTTACTTTCCTTCTGATGATCCCCACAAGTTTGCCATTGTTCACAAGGTCTTTGGTGCCAGCAATGTTAGCAAAATGTTACAGGAGCTTCCGGCTCATCAGAGAGCAGATGCAGTGAGCAGTTTAGTTTATGAAGCAAATGCAAGAATGAGGGACCCAGTGTACGGATGCGTTGGGGCCATCTCTTACCTGCAAAACCAAGTCTCCGAGCTACAAATGCAGCTTGCTGTGGTCCAAGCTGAGATCCTCTGCACCCAAATGCAGCATGAGCCCATGGTCCCTACCCCACAACAGATAATGGACGATCCGGATGATCAGAAATCATATTTTCTCCAGAACAACAATCTCGCCCAGTACCTCAGTTTTCCCTCGTCCAGCAATGTAATCCATGACTCTCTCCAGAGGGAGAACATTTTTGGACACGACATGGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

228

Amino Acids

25.94

Weight (kDa)

6.81

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 64 - 161 8.3e-43 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 302
AccIII TCCGGA 1 cut(s) 553
AclWI GGATC 3 cut(s) 260, 484, 545
AcoI YGGCCR 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 2 cut(s) 410, 602
AfiI CCNNNNNNNGG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 5 cut(s) 160, 334, 457, 469, 485
AluI AGCT 5 cut(s) 160, 334, 457, 469, 485
Alw26I GTCTC 2 cut(s) 78, 454
AlwI GGATC 3 cut(s) 260, 484, 545
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 2 cut(s) 177, 423
ApeKI GCWGC 3 cut(s) 211, 466, 508
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspLEI GCGC 2 cut(s) 197, 227
AspS9I GGNCC 4 cut(s) 400, 423, 478, 523
AsuII TTCGAA 1 cut(s) 165
AvaII GGWCC 3 cut(s) 400, 478, 523
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 198, 478, 520
BccI CCATC 1 cut(s) 434
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 78, 454
BfmI CTRYAG 1 cut(s) 25
BfuAI ACCTGC 1 cut(s) 444
BisI GCNGC 3 cut(s) 212, 467, 509
BlsI GCNGC 3 cut(s) 213, 468, 510
Bme18I GGWCC 3 cut(s) 400, 478, 523
BmgT120I GGNCC 4 cut(s) 400, 423, 478, 523
BmiI GGNNCC 5 cut(s) 304, 401, 402, 424, 525
BmrI ACTGGG 2 cut(s) 398, 592
BmsI GCATC 3 cut(s) 212, 346, 404
BmuI ACTGGG 2 cut(s) 398, 592
BpmI CTGGAG 2 cut(s) 563, 629
Bpu14I TTCGAA 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 2 cut(s) 404, 598
Bse3DI GCAATG 2 cut(s) 316, 631
BseAI TCCGGA 1 cut(s) 553
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 2 cut(s) 419, 562
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 2 cut(s) 316, 631
BseMII CTCAG 2 cut(s) 477, 619
BseNI ACTGG 2 cut(s) 404, 598
BseXI GCAGC 3 cut(s) 198, 478, 520
BsgI GTGCAG 1 cut(s) 481
BshFI GGCC 2 cut(s) 179, 425
BshNI GGYRCC 1 cut(s) 302
BsiSI CCGG 3 cut(s) 180, 338, 554
BslFI GGGAC 2 cut(s) 413, 509
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 78, 454
BsmFI GGGAC 2 cut(s) 413, 509
BsnI GGCC 2 cut(s) 179, 425
Bsp119I TTCGAA 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 519
Bsp13I TCCGGA 1 cut(s) 553
Bsp143I GATC 5 cut(s) 33, 265, 489, 550, 559
Bsp19I CCATGG 1 cut(s) 519
BspANI GGCC 2 cut(s) 179, 425
BspCNI CTCAG 2 cut(s) 478, 618
BspEI TCCGGA 1 cut(s) 553
BspLI GGNNCC 5 cut(s) 304, 401, 402, 424, 525
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 3 cut(s) 260, 484, 545
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 316, 631
BsrI ACTGG 2 cut(s) 404, 598
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 5 cut(s) 33, 265, 489, 550, 559
BssNI GRCGYC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 519
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 307, 471
BstDEI CTNAG 3 cut(s) 17, 486, 605
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 419, 562
BstHHI GCGC 2 cut(s) 197, 227
BstKTI GATC 5 cut(s) 36, 268, 492, 553, 562
BstMAI GTCTC 2 cut(s) 78, 454
BstMBI GATC 5 cut(s) 33, 265, 489, 550, 559
BstMWI GCNNNNNNNGC 5 cut(s) 52, 201, 315, 463, 514
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 198, 478, 520
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BsuRI GGCC 2 cut(s) 179, 425
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 2 cut(s) 419, 562
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 22, 366, 411
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 307, 471
CfoI GCGC 2 cut(s) 197, 227
Cfr13I GGNCC 4 cut(s) 400, 423, 478, 523
CseI GACGC 1 cut(s) 225
Csp6I GTAC 2 cut(s) 409, 601
CviAII CATG 5 cut(s) 175, 512, 520, 635, 673
CviQI GTAC 2 cut(s) 409, 601
DdeI CTNAG 3 cut(s) 17, 486, 605
DpnI GATC 5 cut(s) 35, 267, 491, 552, 561
DpnII GATC 5 cut(s) 33, 265, 489, 550, 559
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 519
Eco47I GGWCC 3 cut(s) 400, 478, 523
EcoO109I RGGNCCY 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 5 cut(s) 178, 515, 523, 638, 676
FaiI YATR 9 cut(s) 39, 77, 176, 378, 513, 521, 571, 636, 674
FaqI GGGAC 2 cut(s) 413, 509
FatI CATG 5 cut(s) 174, 511, 519, 634, 672
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 3 cut(s) 212, 467, 509
FokI GGATG 2 cut(s) 426, 569
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 3 cut(s) 212, 467, 509
GlaI GCGC 2 cut(s) 196, 226
GluI GCNGC 3 cut(s) 212, 467, 509
GsuI CTGGAG 2 cut(s) 563, 629
HaeIII GGCC 2 cut(s) 179, 425
HapII CCGG 3 cut(s) 180, 338, 554
HgaI GACGC 1 cut(s) 225
HhaI GCGC 2 cut(s) 197, 227
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 5 cut(s) 178, 515, 523, 638, 676
Hin6I GCGC 2 cut(s) 195, 225
HinP1I GCGC 2 cut(s) 195, 225
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 86, 638
HpaII CCGG 3 cut(s) 180, 338, 554
Hpy166II GTNNAC 2 cut(s) 289, 409
Hpy188I TCNGA 4 cut(s) 262, 348, 454, 564
Hpy188III TCNNGA 3 cut(s) 554, 580, 646
Hpy8I GTNNAC 2 cut(s) 289, 409
Hpy99I CGWCG 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 267
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 8 cut(s) 204, 237, 359, 389, 439, 466, 498, 508
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 201, 315, 463, 514
HpyF3I CTNAG 3 cut(s) 17, 486, 605
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 5 cut(s) 178, 515, 523, 638, 676
HspAI GCGC 2 cut(s) 195, 225
KflI GGGWCCC 1 cut(s) 400
Kpn2I TCCGGA 1 cut(s) 553
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 5 cut(s) 33, 265, 489, 550, 559
LmnI GCTCC 2 cut(s) 250, 331
Lsp1109I GCAGC 3 cut(s) 198, 478, 520
LweI GCATC 3 cut(s) 212, 346, 404
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 324
MalI GATC 5 cut(s) 35, 267, 491, 552, 561
MboI GATC 5 cut(s) 33, 265, 489, 550, 559
MboII GAAGA 2 cut(s) 124, 130
MflI RGATCY 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 519
MlyI GAGTC 2 cut(s) 80, 632
MnlI CCTC 6 cut(s) 146, 390, 503, 614, 625, 642
MroI TCCGGA 1 cut(s) 553
MseI TTAA 2 cut(s) 50, 682
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 3 cut(s) 180, 338, 554
MwoI GCNNNNNNNGC 5 cut(s) 52, 201, 315, 463, 514
NcoI CCATGG 1 cut(s) 519
NdeII GATC 5 cut(s) 33, 265, 489, 550, 559
NlaIII CATG 5 cut(s) 178, 515, 523, 638, 676
NlaIV GGNNCC 5 cut(s) 304, 401, 402, 424, 525
NspV TTCGAA 1 cut(s) 165
PkrI GCNGC 3 cut(s) 213, 468, 510
PleI GAGTC 2 cut(s) 80, 632
PpsI GAGTC 2 cut(s) 80, 632
PpuMI RGGWCCY 1 cut(s) 400
PsiI TTATAA 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 400
PspN4I GGNNCC 5 cut(s) 304, 401, 402, 424, 525
PspPI GGNCC 4 cut(s) 400, 423, 478, 523
PspPPI RGGWCCY 1 cut(s) 400
PsuI RGATCY 1 cut(s) 489
RsaI GTAC 2 cut(s) 410, 602
RsaNI GTAC 2 cut(s) 409, 601
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 2 cut(s) 50, 682
SatI GCNGC 3 cut(s) 212, 467, 509
Sau3AI GATC 5 cut(s) 33, 265, 489, 550, 559
Sau96I GGNCC 4 cut(s) 400, 423, 478, 523
SchI GAGTC 2 cut(s) 80, 632
SduI GDGCHC 1 cut(s) 519
SetI ASST 9 cut(s) 65, 162, 297, 336, 438, 459, 471, 487, 606
SfaNI GCATC 3 cut(s) 212, 346, 404
SfcI CTRYAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 3 cut(s) 400, 478, 523
SmiMI CAYNNNNRTG 1 cut(s) 503
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 65
TaqI TCGA 1 cut(s) 165
Tru1I TTAA 2 cut(s) 50, 682
Tru9I TTAA 2 cut(s) 50, 682
TscAI CASTG 3 cut(s) 29, 366, 411
TseI GCWGC 3 cut(s) 211, 466, 508
TspDTI ATGAA 2 cut(s) 89, 393
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 3 cut(s) 29, 366, 411
VpaK11BI GGWCC 3 cut(s) 400, 478, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.