RchiOBHm_Chr5g0071311

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002494
Physical Location & Seq
Forward (+)
77200107 .. 77203580
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34641

Sequence Viewer

Length: 588 bp
ATGGCTGGCATTGGAAATCCAACACCACTCTGTTCAAGCAAATTGAAATTTCAGGGTGAAATGGGAGGAACTTCACCATGTGCTTCGTGCAAGTTGTTGAGGCGCCGCTGCGCTAAGGACTGCATCTTTGCCCCTTACTTTCCTTCCGATGACCCTCATAAGTTTGCCATTGTTCACAAGGTCTTTGGTGCTAGCAATGTCAGCAAAATGTTGCAGGAGCTTCCGCTTCATCAGAGAGGAGATGCTGTGAGCAGCTTGGTGTATGAAGCAAATGCAAGAGTGAGAGACCCGGTTTACGGCTGTGTCGGTGCTATATCTTTCCTGCAGAATCAAGTTTCGAATCTGCAAATGCAGCTCGCGGTGGCTCAGGCTGAAATACTCTGCATCCAGATGCAACAAGAGCCAGTTGCAGCGACCTTACCCGCATCGCAGATGCTAGATCATCACCAATATCAAGATCACGATCAGAAATCCCTTCTCTTGTCCAATAGTCACGACTTCAACAACATTCCACAGTACCTCAGCAGCTTTGCTTCTTCCAGCAATGTAATCCAAGACCCTCTTAAGAGAGAGTCTCTATGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

195

Amino Acids

21.52

Weight (kDa)

7.03

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 26 - 123 3.7e-42 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 102
AccII CGCG 1 cut(s) 359
AciI CCGC 4 cut(s) 106, 224, 359, 423
AcsI RAATTY 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 103
AdeI CACNNNGTG 1 cut(s) 80
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 296
AflII CTTAAG 1 cut(s) 563
AgsI TTSAA 3 cut(s) 36, 46, 502
AluBI AGCT 4 cut(s) 220, 255, 355, 528
AluI AGCT 4 cut(s) 220, 255, 355, 528
Alw26I GTCTC 2 cut(s) 279, 579
ApeKI GCWGC 5 cut(s) 108, 252, 352, 410, 525
ApoI RAATTY 1 cut(s) 47
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspLEI GCGC 2 cut(s) 105, 113
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 66, 68, 437
AsuII TTCGAA 1 cut(s) 338
AsuNHI GCTAGC 1 cut(s) 191
BanI GGYRCC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 521
BbvI GCAGC 5 cut(s) 95, 264, 364, 422, 537
BceAI ACGGC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 279, 579
BfaI CTAG 2 cut(s) 192, 437
BfmI CTRYAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 106
BfrI CTTAAG 1 cut(s) 563
BisI GCNGC 6 cut(s) 106, 109, 253, 353, 411, 526
BlsI GCNGC 6 cut(s) 107, 110, 254, 354, 412, 527
Bme1390I CCNGG 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 290
BmsI GCATC 6 cut(s) 132, 232, 381, 393, 423, 434
BmtI GCTAGC 1 cut(s) 195
BplI GAGNNNNNCTC 1 cut(s) 559
Bpu10I CCTNAGC 3 cut(s) 114, 366, 521
Bpu14I TTCGAA 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 103
BsaI GGTCTC 1 cut(s) 279
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 404
Bse3DI GCAATG 2 cut(s) 202, 550
Bse8I GATNNNNATC 1 cut(s) 462
BseGI GGATG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 2 cut(s) 202, 550
BseMII CTCAG 2 cut(s) 380, 535
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 252
BseXI GCAGC 5 cut(s) 95, 264, 364, 422, 537
Bsh1236I CGCG 1 cut(s) 359
BshNI GGYRCC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 2 cut(s) 279, 579
Bso31I GGTCTC 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 338
Bsp143I GATC 3 cut(s) 439, 457, 463
BspACI CCGC 4 cut(s) 106, 224, 359, 423
BspCNI CTCAG 2 cut(s) 379, 534
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 104
BspMAI CTGCAG 1 cut(s) 327
BspOI GCTAGC 1 cut(s) 195
BspT104I TTCGAA 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 102
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 2 cut(s) 202, 550
BsrI ACTGG 1 cut(s) 404
BssMI GATC 3 cut(s) 439, 457, 463
BssNI GRCGYC 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 103
BstAFI CTTAAG 1 cut(s) 563
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 7, 193, 357
BstDEI CTNAG 3 cut(s) 114, 366, 521
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 2 cut(s) 105, 113
BstKTI GATC 3 cut(s) 442, 460, 466
BstMAI GTCTC 2 cut(s) 279, 579
BstMBI GATC 3 cut(s) 439, 457, 463
BstMWI GCNNNNNNNGC 3 cut(s) 201, 352, 400
BstSCI CCNGG 1 cut(s) 288
BstSFI CTRYAG 1 cut(s) 323
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 5 cut(s) 95, 264, 364, 422, 537
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 1 cut(s) 384
Cac8I GCNNGC 3 cut(s) 7, 193, 357
CfoI GCGC 2 cut(s) 105, 113
Csp6I GTAC 1 cut(s) 517
CviAII CATG 1 cut(s) 78
CviJI RGCY 9 cut(s) 5, 220, 255, 300, 355, 365, 371, 403, 528
CviKI_1 RGCY 9 cut(s) 5, 220, 255, 300, 355, 365, 371, 403, 528
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 3 cut(s) 114, 366, 521
DinI GGCGCC 1 cut(s) 104
DpnI GATC 3 cut(s) 441, 459, 465
DpnII GATC 3 cut(s) 439, 457, 463
DraIII CACNNNGTG 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 279
EgeI GGCGCC 1 cut(s) 104
EheI GGCGCC 1 cut(s) 104
FaeI CATG 1 cut(s) 81
FaiI YATR 5 cut(s) 79, 159, 264, 314, 580
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FatI CATG 1 cut(s) 77
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 6 cut(s) 106, 109, 253, 353, 411, 526
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 6 cut(s) 106, 109, 253, 353, 411, 526
FspBI CTAG 2 cut(s) 192, 437
GlaI GCGC 2 cut(s) 104, 112
GluI GCNGC 6 cut(s) 106, 109, 253, 353, 411, 526
HaeII RGCGCY 1 cut(s) 106
HapII CCGG 1 cut(s) 290
HhaI GCGC 2 cut(s) 105, 113
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 1 cut(s) 81
Hin6I GCGC 2 cut(s) 103, 111
HinP1I GCGC 2 cut(s) 103, 111
HinfI GANTC 3 cut(s) 328, 340, 572
HpaII CCGG 1 cut(s) 290
HphI GGTGA 3 cut(s) 66, 68, 437
Hpy166II GTNNAC 2 cut(s) 175, 295
Hpy188I TCNGA 3 cut(s) 148, 234, 468
Hpy188III TCNNGA 4 cut(s) 388, 455, 461, 494
Hpy8I GTNNAC 2 cut(s) 175, 295
HpyAV CCTTC 2 cut(s) 153, 485
HpyCH4III ACNGT 1 cut(s) 516
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 352, 400
HpyF3I CTNAG 3 cut(s) 114, 366, 521
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 1 cut(s) 81
HspAI GCGC 2 cut(s) 103, 111
KasI GGCGCC 1 cut(s) 102
Kzo9I GATC 3 cut(s) 439, 457, 463
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 8 cut(s) 38, 200, 303, 335, 353, 401, 417, 553
Lsp1109I GCAGC 5 cut(s) 95, 264, 364, 422, 537
LweI GCATC 6 cut(s) 132, 232, 381, 393, 423, 434
MaeI CTAG 2 cut(s) 192, 437
MaeIII GTNAC 1 cut(s) 491
MalI GATC 3 cut(s) 441, 459, 465
MboI GATC 3 cut(s) 439, 457, 463
MboII GAAGA 1 cut(s) 528
MluCI AATT 2 cut(s) 41, 47
Mly113I GGCGCC 1 cut(s) 103
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 6 cut(s) 59, 93, 165, 230, 530, 570
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 108
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 201, 352, 400
NarI GGCGCC 1 cut(s) 103
NciI CCSGG 1 cut(s) 290
NdeII GATC 3 cut(s) 439, 457, 463
NheI GCTAGC 1 cut(s) 191
NlaIII CATG 1 cut(s) 81
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 491
NspV TTCGAA 1 cut(s) 338
PfeI GAWTC 2 cut(s) 328, 340
PkrI GCNGC 6 cut(s) 107, 110, 254, 354, 412, 527
PleI GAGTC 1 cut(s) 580
PluTI GGCGCC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 104
PstI CTGCAG 1 cut(s) 327
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 6 cut(s) 106, 109, 253, 353, 411, 526
Sau3AI GATC 3 cut(s) 439, 457, 463
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 1 cut(s) 290
SetI ASST 7 cut(s) 183, 222, 257, 357, 419, 522, 530
SfaNI GCATC 6 cut(s) 132, 232, 381, 393, 423, 434
SfcI CTRYAG 1 cut(s) 323
SfoI GGCGCC 1 cut(s) 104
SfuI TTCGAA 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 2 cut(s) 41, 47
SsiI CCGC 4 cut(s) 106, 224, 359, 423
SspDI GGCGCC 1 cut(s) 102
SspMI CTAG 2 cut(s) 192, 437
StyD4I CCNGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 516
TaqI TCGA 1 cut(s) 338
TasI AATT 2 cut(s) 41, 47
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 328, 340
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 5 cut(s) 108, 252, 352, 410, 525
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 218, 279
Vha464I CTTAAG 1 cut(s) 563
XapI RAATTY 1 cut(s) 47
XspI CTAG 2 cut(s) 192, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.