MD03G1060100.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002494
Physical Location & Seq
Reverse (-)
4847728 .. 4848758
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1060100.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGGATCGGGCTCTTCGCCGTGTGCTTCTTGCAGGTTGTTGAGGCGCCGGTGTGCCAAGGACTGCATCTTTGCCCCATACTTCCCTTCTGATGACCCTTATAAGTTTGCCATTGTTCATAAGGTCTTTGGTGCTAGCAATGTCAGCAAAATGTTGCAGGAGCTTCCCATTCATCAAAGAGGAGATGCTGTGAGCAGTTTGGTTTATGAAGCAAATGCAAGAGTGAGAGACCCGGTTTACGGGTGTGTTGGGGCAATTTCTTTCCTGCAGAATCAAGTTTCTGAGCTGCAAATGCAGCTTGCAGTGGCTCAGGCAGAGACACTTTGCTTCCAGATGCAACAGGATCCTGTAGCCTTACCAACCCAGATTGATCAACACCATCATCAACATCAAGATCATGACCAGAAGTCCCTTCTTTTATCCAACAGTGACTTCAATAGCATTCCACATCAATACTTCAGCAGCTTCGCTTCTCCTAGCAATGTTATCCAAGACCCTCTTAAAAGAGAGTCTCTTTGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

175

Amino Acids

19.51

Weight (kDa)

6.42

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 7 - 104 1.8e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 45
AclWI GGATC 3 cut(s) 13, 338, 351
AcuI CTGAAG 1 cut(s) 442
AcyI GRCGYC 1 cut(s) 46
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 436
AhdI GACNNNNNGTC 1 cut(s) 406
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 4 cut(s) 163, 286, 298, 465
AluI AGCT 4 cut(s) 163, 286, 298, 465
Alw26I GTCTC 3 cut(s) 222, 311, 516
AlwI GGATC 3 cut(s) 13, 338, 351
ApeKI GCWGC 3 cut(s) 286, 295, 462
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 233
AsuNHI GCTAGC 1 cut(s) 134
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 273, 307, 474
BccI CCATC 1 cut(s) 387
BceAI ACGGC 1 cut(s) 4
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 222, 311, 516
BfaI CTAG 2 cut(s) 135, 477
BfmI CTRYAG 2 cut(s) 266, 348
BfoI RGCGCY 1 cut(s) 49
BfuAI ACCTGC 1 cut(s) 24
BisI GCNGC 3 cut(s) 287, 296, 463
BlsI GCNGC 3 cut(s) 288, 297, 464
Bme1390I CCNGG 1 cut(s) 233
BmeRI GACNNNNNGTC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 47, 345
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 3 cut(s) 75, 175, 324
BmtI GCTAGC 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 48
Bse3DI GCAATG 2 cut(s) 145, 487
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 2 cut(s) 145, 487
BseMII CTCAG 2 cut(s) 273, 323
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 3 cut(s) 273, 307, 474
BshNI GGYRCC 1 cut(s) 45
BsiSI CCGG 2 cut(s) 49, 233
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 3 cut(s) 222, 311, 516
BsmFI GGGAC 1 cut(s) 394
BsmI GAATGC 1 cut(s) 441
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 4 cut(s) 5, 343, 370, 394
BspCNI CTCAG 2 cut(s) 274, 322
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 47, 345
BspMAI CTGCAG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 24
BspOI GCTAGC 1 cut(s) 138
BspPI GGATC 3 cut(s) 13, 338, 351
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 2 cut(s) 145, 487
BsrFI RCCGGY 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 4 cut(s) 5, 343, 370, 394
BssNI GRCGYC 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 136, 300
BstDEI CTNAG 2 cut(s) 282, 309
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 4 cut(s) 8, 346, 373, 397
BstMAI GTCTC 3 cut(s) 222, 311, 516
BstMBI GATC 4 cut(s) 5, 343, 370, 394
BstMWI GCNNNNNNNGC 3 cut(s) 144, 292, 295
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 2 cut(s) 266, 348
BstV1I GCAGC 3 cut(s) 273, 307, 474
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 309, 433
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 136, 300
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 48
CviAII CATG 1 cut(s) 398
CviJI RGCY 7 cut(s) 12, 163, 286, 298, 308, 353, 465
CviKI_1 RGCY 7 cut(s) 12, 163, 286, 298, 308, 353, 465
DdeI CTNAG 2 cut(s) 282, 309
DinI GGCGCC 1 cut(s) 47
DpnI GATC 4 cut(s) 7, 345, 372, 396
DpnII GATC 4 cut(s) 5, 343, 370, 394
DriI GACNNNNNGTC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 14
EgeI GGCGCC 1 cut(s) 47
EheI GGCGCC 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 1 cut(s) 401
FaiI YATR 5 cut(s) 79, 102, 120, 207, 399
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 1 cut(s) 394
FatI CATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 287, 296, 463
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 3 cut(s) 287, 296, 463
FspBI CTAG 2 cut(s) 135, 477
GlaI GCGC 1 cut(s) 47
GluI GCNGC 3 cut(s) 287, 296, 463
HaeII RGCGCY 1 cut(s) 49
HapII CCGG 2 cut(s) 49, 233
HhaI GCGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HinfI GANTC 2 cut(s) 271, 509
HpaII CCGG 2 cut(s) 49, 233
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 91, 283
Hpy188III TCNNGA 3 cut(s) 331, 392, 398
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 2 cut(s) 96, 422
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4V TGCA 9 cut(s) 33, 66, 157, 218, 268, 289, 295, 302, 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 292, 295
HpyF3I CTNAG 2 cut(s) 282, 309
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 1 cut(s) 46
KasI GGCGCC 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 5, 343, 370, 394
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 3 cut(s) 273, 307, 474
LweI GCATC 3 cut(s) 75, 175, 324
MaeI CTAG 2 cut(s) 135, 477
MaeIII GTNAC 1 cut(s) 428
MalI GATC 4 cut(s) 7, 345, 372, 396
MboI GATC 4 cut(s) 5, 343, 370, 394
MboII GAAGA 1 cut(s) 6
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 1 cut(s) 255
Mly113I GGCGCC 1 cut(s) 46
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 3 cut(s) 36, 173, 507
MseI TTAA 1 cut(s) 501
MspI CCGG 2 cut(s) 49, 233
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 441
MwoI GCNNNNNNNGC 3 cut(s) 144, 292, 295
NarI GGCGCC 1 cut(s) 46
NciI CCSGG 1 cut(s) 233
NdeII GATC 4 cut(s) 5, 343, 370, 394
NheI GCTAGC 1 cut(s) 134
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 47, 345
NmuCI GTSAC 1 cut(s) 428
PagI TCATGA 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 3 cut(s) 288, 297, 464
PleI GAGTC 1 cut(s) 517
PluTI GGCGCC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 517
PsiI TTATAA 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 47, 345
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 1 cut(s) 343
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 3 cut(s) 287, 296, 463
Sau3AI GATC 4 cut(s) 5, 343, 370, 394
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 14
SetI ASST 6 cut(s) 38, 126, 165, 288, 300, 467
SfaNI GCATC 3 cut(s) 75, 175, 324
SfcI CTRYAG 2 cut(s) 266, 348
SfoI GGCGCC 1 cut(s) 47
SgrAI CRCCGGYG 1 cut(s) 48
Sse9I AATT 1 cut(s) 255
SspDI GGCGCC 1 cut(s) 45
SspMI CTAG 2 cut(s) 135, 477
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 428
TasI AATT 1 cut(s) 255
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 309, 433
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 3 cut(s) 286, 295, 462
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 3 cut(s) 107, 161, 222
TspRI CASTG 2 cut(s) 309, 433
XspI CTAG 2 cut(s) 135, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.