MD01G1172600.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001828
Physical Location & Seq
Forward (+)
27464216 .. 27467383
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1172600.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGAAGGGGGAAGGAGAATCTCGGCGAGTCCTCGGCCTTGCAACGGTAGGAGAGTGGTGGCGAAGAAACGACCTCGTATTGGTGGGGTTGATGGGTTCGTCAACAGCGTTAAGAAGCTTCAGAGGCGCGAAATCAGCTCCAAGCGCGACCGTGCTTTCACTATGAGCGATGCCCAAGAGAGGTTTCGCAATATTCGCCTCCAGGATGAATATGATACGCATGATCCGAAAGGTCATTGTGCCATGGTTCTGCCTTTCCTGCGAAAGAGGTCCAAAATTATTGAAATTGTAGCAGCACGTGACATTGTGTTTGCCCTCGCACAGTCTGGTGTTTGTGCAGCATTTAGCCGAGAAACCAACCAGAGAATATGCTTTCTGAATGTCAGTCCTGATGAAGTGATAAGAAGCTTGTTTTACAATAAAAACAATGACTCACTTATCACTGTCTCGGTTTATGCTTCAGACAACTTCAGTTCTTTGAAATGCCGAAGCACAAGGATTGAATACATACGAAGGGGTAAACCAGATGCTGGATTTGCTCTTTTTGAATCCGAGTCACTGAAATGGCCTGGTTTTGTAGAGTTCGATGATGTAAATGGGAAGGTTCTTACTTATTCTGCACAGGATAGTATATACAAGGTGTTTGATCTGAAAAACTATACAATGTTGTACTCCATATCGGATAAGCATGTCCAAGAGATTAAGATCAGTCCTGGGATCATGCTGTTGATTTTTACTAAAGCTAGCAGTCATGTTCCTCTTAAGATTCTGTCTATAGAGGATGGTACTGTTCTCAAATCCTTTAACCATCTCCTTCATCGGAATAAGAAGGTGGATTTCATCGAACAGTTCAACGAGAAGCTTCTTGTAAAGCAAGAAAATGAGAACCTTCAAATTCTTGATGTTCGCAATTTTGAGATGACAGAAGTAAGCAGAACTCAATTCATGACCCCATCAGCCTTCATATTTCTATATGAGAATCAGTTATTCTTGACGTTTCGGAATCGAACAGTGGCTGTTTGGAACTTTAGAGGAGAACTTGTGACTTCATTTGAGGATCATCTTTTGTGGCATCCCGACTGCAATACCAATAACATATATATTACTAGTGACCAGGATCTTATTATTTCTTACTGCAAGGCTGATTCTGATGATCCATTGTCGGAAGGAAATGCCGGATCCATCAATATCAGCAATATCTTGACTGGAAAATGCCTTGCTAAGATAAGAGCTACCAACAACGCTGAAAAAGAATGCATTTGTAGTGGTGAGGCTTGTGGCAGCAGTTGCAATTCAAAGAAGCGGTCCCTTTCCTCCCGAATTAGGAGTACAGTTGCAGAAGCCTTGGAAGACATTACTGCCCTCTTCTACGACGAAGAGCGCAACGAGATCTACACGGGCAATAGGCTCGGTCTAGTTCACGTATGGTCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

479

Amino Acids

54.5

Weight (kDa)

8.7

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 51 - 476 2e-226 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 530
AccII CGCG 2 cut(s) 129, 147
AciI CCGC 1 cut(s) 1303
AclWI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
AcsI RAATTY 1 cut(s) 894
AcuI CTGAAG 3 cut(s) 104, 444, 454
AcvI CACGTG 1 cut(s) 299
AfaI GTAC 3 cut(s) 671, 787, 1330
AfiI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 530, 1323
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 7 cut(s) 284, 481, 503, 548, 853, 893, 1296
AhlI ACTAGT 1 cut(s) 1106
AjnI CCWGG 4 cut(s) 201, 568, 712, 1113
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 6 cut(s) 118, 138, 408, 743, 862, 1232
AluI AGCT 6 cut(s) 118, 138, 408, 743, 862, 1232
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
AlwNI CAGNNNCTG 2 cut(s) 530, 1016
AoxI GGCC 2 cut(s) 35, 566
ApeKI GCWGC 3 cut(s) 293, 338, 1281
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 4 cut(s) 293, 325, 1289, 1321
AspLEI GCGC 3 cut(s) 129, 147, 1383
AspS9I GGNCC 2 cut(s) 270, 1305
AsuHPI GGTGA 1 cut(s) 1280
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 2 cut(s) 270, 1305
BamHI GGATCC 1 cut(s) 1178
BbrPI CACGTG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 1356
BbvI GCAGC 3 cut(s) 305, 350, 1293
BccI CCATC 5 cut(s) 86, 776, 816, 961, 1190
BcgI CGANNNNNNTGC 2 cut(s) 1390, 1424
BciT130I CCWGG 4 cut(s) 203, 570, 714, 1115
BcoDI GTCTC 1 cut(s) 451
BcuI ACTAGT 1 cut(s) 1106
BfaI CTAG 3 cut(s) 744, 1107, 1415
BfmI CTRYAG 1 cut(s) 774
BfrI CTTAAG 1 cut(s) 761
BglII AGATCT 1 cut(s) 1389
BisI GCNGC 3 cut(s) 294, 339, 1282
BlsI GCNGC 3 cut(s) 295, 340, 1283
Bme1390I CCNGG 4 cut(s) 203, 570, 714, 1115
Bme18I GGWCC 2 cut(s) 270, 1305
BmgT120I GGNCC 2 cut(s) 270, 1305
BmiI GGNNCC 2 cut(s) 1180, 1307
BmrFI CCNGG 4 cut(s) 203, 570, 714, 1115
BmsI GCATC 3 cut(s) 160, 517, 1081
BmtI GCTAGC 1 cut(s) 747
BpiI GAAGAC 1 cut(s) 1356
BpmI CTGGAG 1 cut(s) 185
BsaAI YACGTR 2 cut(s) 299, 1423
BsaBI GATNNNNATC 1 cut(s) 704
BsaJI CCNNGG 4 cut(s) 32, 243, 713, 1344
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 80, 180, 530, 1323
Bse1I ACTGG 1 cut(s) 1210
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 4 cut(s) 203, 570, 714, 1115
BseDI CCNNGG 4 cut(s) 32, 243, 713, 1344
BseGI GGATG 3 cut(s) 211, 787, 1072
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 530, 1323
BseNI ACTGG 1 cut(s) 1210
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 3 cut(s) 305, 350, 1293
BsgI GTGCAG 2 cut(s) 357, 603
Bsh1236I CGCG 2 cut(s) 129, 147
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 2 cut(s) 37, 568
BsiEI CGRYCG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 1176
BslFI GGGAC 1 cut(s) 1291
BslI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 530, 1323
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 1291
BsmI GAATGC 1 cut(s) 1259
BsnI GGCC 2 cut(s) 37, 568
Bsp143I GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 1 cut(s) 1303
BspANI GGCC 2 cut(s) 37, 568
BspFNI CGCG 2 cut(s) 129, 147
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 1180, 1307
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
BspQI GCTCTTC 1 cut(s) 1371
BspTI CTTAAG 1 cut(s) 761
BsrI ACTGG 1 cut(s) 1210
BssECI CCNNGG 4 cut(s) 32, 243, 713, 1344
BssMI GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
BssT1I CCWWGG 2 cut(s) 243, 1344
Bst2UI CCWGG 4 cut(s) 203, 570, 714, 1115
Bst4CI ACNGT 8 cut(s) 47, 152, 324, 445, 790, 849, 1012, 1333
Bst6I CTCTTC 2 cut(s) 1370, 1371
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 1287
BstBAI YACGTR 2 cut(s) 299, 1423
BstC8I GCNNGC 1 cut(s) 745
BstDEI CTNAG 1 cut(s) 1221
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 3 cut(s) 211, 787, 1072
BstFNI CGCG 2 cut(s) 129, 147
BstHHI GCGC 3 cut(s) 129, 147, 1383
BstKTI GATC 9 cut(s) 226, 649, 708, 720, 1060, 1120, 1156, 1181, 1392
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 7 cut(s) 124, 135, 144, 195, 259, 536, 1287
BstNI CCWGG 4 cut(s) 203, 570, 714, 1115
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 4 cut(s) 201, 568, 712, 1113
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 2 cut(s) 129, 147
BstV1I GCAGC 3 cut(s) 305, 350, 1293
BstV2I GAAGAC 1 cut(s) 1356
BstX2I RGATCY 3 cut(s) 1117, 1178, 1389
BstYI RGATCY 3 cut(s) 1117, 1178, 1389
BsuRI GGCC 2 cut(s) 37, 568
BtgI CCRYGG 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 3 cut(s) 211, 787, 1072
BtsIMutI CAGTG 3 cut(s) 441, 557, 1017
Cac8I GCNNGC 1 cut(s) 745
CaiI CAGNNNCTG 2 cut(s) 530, 1016
CciI TCATGA 1 cut(s) 945
CfoI GCGC 3 cut(s) 129, 147, 1383
Cfr13I GGNCC 2 cut(s) 270, 1305
Csp6I GTAC 3 cut(s) 670, 786, 1329
CviAII CATG 6 cut(s) 221, 244, 689, 721, 752, 946
CviQI GTAC 3 cut(s) 670, 786, 1329
DdeI CTNAG 1 cut(s) 1221
DpnI GATC 9 cut(s) 225, 648, 707, 719, 1059, 1119, 1155, 1180, 1391
DpnII GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
Eam1104I CTCTTC 2 cut(s) 1370, 1371
EarI CTCTTC 2 cut(s) 1370, 1371
Eco130I CCWWGG 2 cut(s) 243, 1344
Eco47I GGWCC 2 cut(s) 270, 1305
Eco57I CTGAAG 3 cut(s) 104, 444, 454
Eco72I CACGTG 1 cut(s) 299
EcoRII CCWGG 4 cut(s) 201, 568, 712, 1113
EcoT14I CCWWGG 2 cut(s) 243, 1344
EcoT22I ATGCAT 1 cut(s) 1259
ErhI CCWWGG 2 cut(s) 243, 1344
FaeI CATG 6 cut(s) 224, 247, 692, 724, 755, 949
FaqI GGGAC 1 cut(s) 1291
FatI CATG 6 cut(s) 220, 243, 688, 720, 751, 945
Fnu4HI GCNGC 3 cut(s) 294, 339, 1282
FokI GGATG 3 cut(s) 218, 794, 1059
Fsp4HI GCNGC 3 cut(s) 294, 339, 1282
FspBI CTAG 3 cut(s) 744, 1107, 1415
GlaI GCGC 3 cut(s) 128, 146, 1382
GluI GCNGC 3 cut(s) 294, 339, 1282
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 2 cut(s) 37, 568
HapII CCGG 1 cut(s) 1176
HhaI GCGC 3 cut(s) 129, 147, 1383
Hin1II CATG 6 cut(s) 224, 247, 692, 724, 755, 949
Hin6I GCGC 3 cut(s) 127, 145, 1381
HinP1I GCGC 3 cut(s) 127, 145, 1381
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 3 cut(s) 116, 406, 860
HinfI GANTC 9 cut(s) 18, 28, 431, 548, 554, 766, 979, 1003, 1145
HpaII CCGG 1 cut(s) 1176
HphI GGTGA 1 cut(s) 1280
Hpy166II GTNNAC 3 cut(s) 103, 521, 1420
Hpy188III TCNNGA 7 cut(s) 389, 899, 946, 991, 1076, 1201, 1317
Hpy8I GTNNAC 3 cut(s) 103, 521, 1420
Hpy99I CGWCG 1 cut(s) 1376
HpyAV CCTTC 8 cut(s) 6, 507, 595, 823, 824, 899, 970, 1160
HpyCH4III ACNGT 8 cut(s) 47, 152, 324, 445, 790, 849, 1012, 1333
HpyCH4IV ACGT 3 cut(s) 298, 995, 1422
HpyCH4V TGCA 8 cut(s) 42, 338, 620, 1083, 1137, 1257, 1290, 1337
HpyF10VI GCNNNNNNNGC 7 cut(s) 124, 135, 144, 195, 259, 536, 1287
HpyF3I CTNAG 1 cut(s) 1221
HpySE526I ACGT 3 cut(s) 298, 995, 1422
Hsp92II CATG 6 cut(s) 224, 247, 692, 724, 755, 949
HspAI GCGC 3 cut(s) 127, 145, 1381
Kzo9I GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 3 cut(s) 305, 350, 1293
LweI GCATC 3 cut(s) 160, 517, 1081
MaeI CTAG 3 cut(s) 744, 1107, 1415
MaeII ACGT 3 cut(s) 298, 995, 1422
MaeIII GTNAC 4 cut(s) 299, 555, 1042, 1109
MalI GATC 9 cut(s) 225, 648, 707, 719, 1059, 1119, 1155, 1180, 1391
MboI GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
MboII GAAGA 4 cut(s) 76, 1357, 1361, 1388
MflI RGATCY 3 cut(s) 1117, 1178, 1389
MluCI AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
MlyI GAGTC 3 cut(s) 37, 425, 563
MmeI TCCRAC 1 cut(s) 1143
Mph1103I ATGCAT 1 cut(s) 1259
MseI TTAA 4 cut(s) 111, 702, 762, 804
MslI CAYNNNNRTG 1 cut(s) 562
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 1 cut(s) 1176
MspR9I CCNGG 4 cut(s) 203, 570, 714, 1115
Mva1269I GAATGC 1 cut(s) 1259
MvaI CCWGG 4 cut(s) 203, 570, 714, 1115
MvnI CGCG 2 cut(s) 129, 147
MwoI GCNNNNNNNGC 7 cut(s) 124, 135, 144, 195, 259, 536, 1287
NcoI CCATGG 1 cut(s) 243
NdeII GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 6 cut(s) 224, 247, 692, 724, 755, 949
NlaIV GGNNCC 2 cut(s) 1180, 1307
NmeAIII GCCGAG 2 cut(s) 13, 374
NmuCI GTSAC 4 cut(s) 299, 555, 1042, 1109
NsiI ATGCAT 1 cut(s) 1259
NspI RCATGY 1 cut(s) 692
PagI TCATGA 1 cut(s) 945
PciSI GCTCTTC 1 cut(s) 1371
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 224
PctI GAATGC 1 cut(s) 1259
PfeI GAWTC 6 cut(s) 18, 548, 766, 979, 1003, 1145
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 3 cut(s) 295, 340, 1283
PleI GAGTC 3 cut(s) 36, 425, 562
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
PpsI GAGTC 3 cut(s) 36, 425, 562
Ppu21I YACGTR 2 cut(s) 299, 1423
Psp6I CCWGG 4 cut(s) 201, 568, 712, 1113
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 4 cut(s) 201, 568, 712, 1113
PspN4I GGNNCC 2 cut(s) 1180, 1307
PspPI GGNCC 2 cut(s) 270, 1305
PstNI CAGNNNCTG 2 cut(s) 530, 1016
PsuI RGATCY 3 cut(s) 1117, 1178, 1389
RsaI GTAC 3 cut(s) 671, 787, 1330
RsaNI GTAC 3 cut(s) 670, 786, 1329
RseI CAYNNNNRTG 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 4 cut(s) 111, 702, 762, 804
SatI GCNGC 3 cut(s) 294, 339, 1282
Sau3AI GATC 9 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178, 1389
Sau96I GGNCC 2 cut(s) 270, 1305
SchI GAGTC 3 cut(s) 37, 425, 563
ScrFI CCNGG 4 cut(s) 203, 570, 714, 1115
SfaNI GCATC 3 cut(s) 160, 517, 1081
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 2 cut(s) 270, 1305
SmiMI CAYNNNNRTG 1 cut(s) 562
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SpeI ACTAGT 1 cut(s) 1106
Sse9I AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
SsiI CCGC 1 cut(s) 1303
SspI AATATT 1 cut(s) 193
SspMI CTAG 3 cut(s) 744, 1107, 1415
StyD4I CCNGG 4 cut(s) 201, 568, 712, 1113
StyI CCWWGG 2 cut(s) 243, 1344
TaaI ACNGT 8 cut(s) 47, 152, 324, 445, 790, 849, 1012, 1333
TaiI ACGT 3 cut(s) 301, 998, 1425
TaqI TCGA 3 cut(s) 585, 843, 1006
TaqII GACCGA 1 cut(s) 1400
TasI AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
TatI WGTACW 2 cut(s) 669, 1328
TfiI GAWTC 6 cut(s) 18, 548, 766, 979, 1003, 1145
Tru1I TTAA 4 cut(s) 111, 702, 762, 804
Tru9I TTAA 4 cut(s) 111, 702, 762, 804
TscAI CASTG 3 cut(s) 448, 564, 1017
TseFI GTSAC 4 cut(s) 299, 555, 1042, 1109
TseI GCWGC 3 cut(s) 293, 338, 1281
Tsp45I GTSAC 4 cut(s) 299, 555, 1042, 1109
TspDTI ATGAA 7 cut(s) 222, 408, 806, 829, 934, 952, 1038
TspRI CASTG 3 cut(s) 448, 564, 1017
Van91I CCANNNNNTGG 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 2 cut(s) 270, 1305
XapI RAATTY 1 cut(s) 894
XceI RCATGY 1 cut(s) 692
XspI CTAG 3 cut(s) 744, 1107, 1415
Zsp2I ATGCAT 1 cut(s) 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.