Rroxscaffold_6G00420170
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001828
Physical Location & Seq
Forward (+)
41496460 .. 41500933
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420170.1

Sequence Viewer

Length: 1419 bp
ATGGAAGTCGGGAGGAGGATATCGGCAAGTCCTCGGCCGTGCTCCGGGCGGCGAGTGGTGGCGAGGAAGAAGAGGGCCGCCGGAGATGGGTTCGTCAACAGCGTCAAGAAGCTTCAGAGGCGTGAGATTTGTTCAAAGCGTGATCGAGCCTTTGGGATGAGCAATGCCCAAGAGAGATTCCGCAACATGCACTTGATGGAGGAATATGATACTCATGATCCCAAGGGTCATTTGTCTTCCAAATTGCCTTTTCTGATGAAGAGGACAAAGGTAATTGAGATAGTTGCGGCACGTGACATAGTCTTCGCTCTTGCACACTCTGGTGTATGTGCAGCTTTTAGTAGAGAGACAAATGAGAGGATCTGTTTCTTGAATGTTAGTCCTGATGAAGTCATCCGAAGCTTGTTCTATAACAAGAACAATGATTCGCTCATCACAGTTTCTGTCTATGCTTCGGACAACTTCAGCTCTTTGAAATGCAGATCTACAAGGATCGAGTATATAAGGAGGGGCAAGCCAGATGCTGGTTTTGCTCTTTTTGAGTCCGAGTCCTTGAAGTGGCCTGGGTTTGTAGAGTTTGATGATGTTAATGGCAAGGTCTTAACATACTCTGCACAAGATAGTATATATAAGGTGTTCGACCTTAAAAACTATACCATGCTTTACTCTATATCAGACAAAAATGTACAAGAAATCAAGATCAGTCCAGGTATCATGCTGCTGATTTTTAATAAAGCTAGCAGCCATGTTCCGCTGAAGATTCTGTCAATAGAAGATGGCACAGTTCTCAAAGCGTTTAATCATCTGCTTCATCGAAATAAGAAGGTGGATTTCATAGAACAATTCAACGAAAAGCTTCTCGTTAAGCAAGAAAATGAGAATCTTCAGATTCTTGATGTTCGAAATGCGGAGCTGATGGAAGTTAACAGAGCTGAGTTCATGACTCCATCAGCATTTATCTTTCTGTATGAGAACCAGCTATTCCTGACATTCAGAAATAGGACTGTGGCTGTCTGGAACTTTCGTGGAGAACTTGTAACTTCATTTGAGGATCACTTGTTGTGGCATCCTGACTGCAATACCAATAACATTTATATAACAAGTGATCAGGATTTGATCATATCCTACTGCAAAGCTGATTCTGATGATCATTGGATGGAACCAAATGCTGGTTCTATTCATGTCAGCAATATCTTAACCGGGAAATGCCTGGCCAAAATAAATGCTAGCAATGCTGGTCCCAAGGTTGATGAGTGCAGCAGCAGTTCTAGCACCTCAAGGAAGCGGATCAACTCATCTCTAGTGAGAAGCACTGTTGCTGAGGCCTTGGAAGACATCACTGCCCTTTTTTATGATGAAGAACGCAATGAGATATATACTGGAAATAGGCATGGTCTTGTTCATGTGTGGTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

472

Amino Acids

53.9

Weight (kDa)

8.44

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 49 - 470 8.3e-226 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 524, 1169
AciI CCGC 7 cut(s) 49, 78, 181, 287, 752, 908, 1285
AclWI GGATC 5 cut(s) 212, 368, 500, 1059, 1295
AcoI YGGCCR 2 cut(s) 35, 1212
AcuI CTGAAG 4 cut(s) 98, 448, 776, 869
AcvI CACGTG 1 cut(s) 293
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 5 cut(s) 44, 87, 524, 558, 1169
AgsI TTSAA 5 cut(s) 135, 373, 475, 556, 847
AjnI CCWGG 3 cut(s) 562, 706, 1209
AjuI GAANNNNNNNTTGG 2 cut(s) 1156, 1188
AleI CACNNNNGTG 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 5 cut(s) 212, 368, 500, 1059, 1295
AlwNI CAGNNNCTG 2 cut(s) 443, 524
AoxI GGCC 5 cut(s) 35, 75, 560, 1212, 1323
ApeKI GCWGC 5 cut(s) 332, 718, 741, 1257, 1260
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 1 cut(s) 855
AspS9I GGNCC 2 cut(s) 75, 1238
AsuC2I CCSGG 2 cut(s) 46, 1201
AsuII TTCGAA 1 cut(s) 901
AsuNHI GCTAGC 2 cut(s) 737, 1226
AvaII GGWCC 1 cut(s) 1238
BalI TGGCCA 1 cut(s) 1214
BbrPI CACGTG 1 cut(s) 293
BbsI GAAGAC 3 cut(s) 228, 295, 1338
Bbv12I GWGCWC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 1320
BbvI GCAGC 5 cut(s) 344, 705, 753, 1269, 1272
BccI CCATC 6 cut(s) 80, 190, 770, 910, 955, 1150
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 3 cut(s) 564, 708, 1211
BclI TGATCA 3 cut(s) 1105, 1116, 1147
BcnI CCSGG 2 cut(s) 46, 1201
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 4 cut(s) 738, 1227, 1269, 1301
BglII AGATCT 1 cut(s) 482
BisI GCNGC 8 cut(s) 50, 78, 288, 333, 719, 742, 1258, 1261
BlsI GCNGC 8 cut(s) 51, 79, 289, 334, 720, 743, 1259, 1262
Bme1390I CCNGG 5 cut(s) 46, 564, 708, 1201, 1211
Bme18I GGWCC 1 cut(s) 1238
BmgT120I GGNCC 2 cut(s) 75, 1238
BmiI GGNNCC 2 cut(s) 1161, 1240
BmrFI CCNGG 5 cut(s) 46, 564, 708, 1201, 1211
BmsI GCATC 2 cut(s) 511, 1075
BmtI GCTAGC 2 cut(s) 741, 1230
BpiI GAAGAC 3 cut(s) 228, 295, 1338
Bpu10I CCTNAGC 1 cut(s) 1320
Bpu14I TTCGAA 1 cut(s) 901
BpuEI CTTGAG 1 cut(s) 1261
BpuMI CCSGG 2 cut(s) 46, 1201
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 32, 222, 563, 1242, 1326
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 87, 524, 558, 1169
Bse1I ACTGG 1 cut(s) 1384
Bse3DI GCAATG 3 cut(s) 169, 1237, 1372
BseBI CCWGG 3 cut(s) 564, 708, 1211
BseDI CCNNGG 5 cut(s) 32, 222, 563, 1242, 1326
BseGI GGATG 4 cut(s) 162, 393, 1066, 1161
BseLI CCNNNNNNNGG 5 cut(s) 44, 87, 524, 558, 1169
BseMI GCAATG 3 cut(s) 169, 1237, 1372
BseMII CTCAG 2 cut(s) 924, 1311
BseNI ACTGG 1 cut(s) 1384
BseRI GAGGAG 1 cut(s) 28
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 5 cut(s) 344, 705, 753, 1269, 1272
BsgI GTGCAG 3 cut(s) 351, 597, 1276
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 5 cut(s) 37, 77, 562, 1214, 1325
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 3 cut(s) 45, 81, 1200
BslFI GGGAC 1 cut(s) 1224
BslI CCNNNNNNNGG 5 cut(s) 44, 87, 524, 558, 1169
BsmAI GTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 1224
BsnI GGCC 5 cut(s) 37, 77, 562, 1214, 1325
Bsp119I TTCGAA 1 cut(s) 901
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 685
BspACI CCGC 7 cut(s) 49, 78, 181, 287, 752, 908, 1285
BspANI GGCC 5 cut(s) 37, 77, 562, 1214, 1325
BspCNI CTCAG 2 cut(s) 925, 1312
BspHI TCATGA 2 cut(s) 214, 939
BspLI GGNNCC 2 cut(s) 1161, 1240
BspOI GCTAGC 2 cut(s) 741, 1230
BspPI GGATC 5 cut(s) 212, 368, 500, 1059, 1295
BspT104I TTCGAA 1 cut(s) 901
BsrDI GCAATG 3 cut(s) 169, 1237, 1372
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 1 cut(s) 1384
BssECI CCNNGG 5 cut(s) 32, 222, 563, 1242, 1326
BssT1I CCWWGG 3 cut(s) 222, 1242, 1326
Bst2UI CCWGG 3 cut(s) 564, 708, 1211
Bst4CI ACNGT 4 cut(s) 439, 784, 1006, 1315
Bst6I CTCTTC 2 cut(s) 65, 254
BstAUI TGTACA 1 cut(s) 685
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 901
BstC8I GCNNGC 3 cut(s) 515, 739, 1228
BstDEI CTNAG 2 cut(s) 933, 1320
BstF5I GGATG 4 cut(s) 162, 393, 1066, 1161
BstMAI GTCTC 1 cut(s) 341
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 4 cut(s) 118, 530, 1232, 1269
BstNI CCWGG 3 cut(s) 564, 708, 1211
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 5 cut(s) 44, 562, 706, 1199, 1209
BstV1I GCAGC 5 cut(s) 344, 705, 753, 1269, 1272
BstV2I GAAGAC 3 cut(s) 228, 295, 1338
BstX2I RGATCY 2 cut(s) 360, 482
BstYI RGATCY 2 cut(s) 360, 482
BstZI CGGCCG 1 cut(s) 35
BsuRI GGCC 5 cut(s) 37, 77, 562, 1214, 1325
BtsCI GGATG 4 cut(s) 162, 393, 1066, 1161
BtsI GCAGTG 1 cut(s) 1338
BtsIMutI CAGTG 2 cut(s) 1311, 1338
Cac8I GCNNGC 3 cut(s) 515, 739, 1228
CaiI CAGNNNCTG 2 cut(s) 443, 524
CciI TCATGA 2 cut(s) 214, 939
Cfr13I GGNCC 2 cut(s) 75, 1238
CseI GACGC 1 cut(s) 91
Csp6I GTAC 1 cut(s) 686
CviAII CATG 9 cut(s) 187, 215, 658, 715, 746, 940, 1181, 1391, 1403
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 933, 1320
EaeI YGGCCR 2 cut(s) 35, 1212
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 2 cut(s) 65, 254
EarI CTCTTC 2 cut(s) 65, 254
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 3 cut(s) 222, 1242, 1326
Eco147I AGGCCT 1 cut(s) 1325
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 1238
Eco52I CGGCCG 1 cut(s) 35
Eco57I CTGAAG 4 cut(s) 98, 448, 776, 869
Eco72I CACGTG 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 562, 706, 1209
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 3 cut(s) 222, 1242, 1326
ErhI CCWWGG 3 cut(s) 222, 1242, 1326
FaeI CATG 9 cut(s) 190, 218, 661, 718, 749, 943, 1184, 1394, 1406
FaqI GGGAC 1 cut(s) 1224
FatI CATG 9 cut(s) 186, 214, 657, 714, 745, 939, 1180, 1390, 1402
FbaI TGATCA 3 cut(s) 1105, 1116, 1147
Fnu4HI GCNGC 8 cut(s) 50, 78, 288, 333, 719, 742, 1258, 1261
FokI GGATG 4 cut(s) 169, 380, 1053, 1168
Fsp4HI GCNGC 8 cut(s) 50, 78, 288, 333, 719, 742, 1258, 1261
FspBI CTAG 4 cut(s) 738, 1227, 1269, 1301
GluI GCNGC 8 cut(s) 50, 78, 288, 333, 719, 742, 1258, 1261
HaeIII GGCC 5 cut(s) 37, 77, 562, 1214, 1325
HapII CCGG 3 cut(s) 45, 81, 1200
HgaI GACGC 1 cut(s) 91
Hin1II CATG 9 cut(s) 190, 218, 661, 718, 749, 943, 1184, 1394, 1406
HincII GTYRAC 2 cut(s) 97, 925
HindII GTYRAC 2 cut(s) 97, 925
HindIII AAGCTT 3 cut(s) 110, 400, 854
HinfI GANTC 9 cut(s) 177, 425, 542, 548, 760, 880, 889, 943, 1139
HpaI GTTAAC 1 cut(s) 925
HpaII CCGG 3 cut(s) 45, 81, 1200
Hpy166II GTNNAC 2 cut(s) 97, 925
Hpy188I TCNGA 9 cut(s) 117, 255, 398, 457, 547, 676, 888, 995, 1144
Hpy8I GTNNAC 2 cut(s) 97, 925
HpyAV CCTTC 1 cut(s) 817
HpyCH4III ACNGT 4 cut(s) 439, 784, 1006, 1315
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 8 cut(s) 190, 314, 332, 480, 614, 1077, 1131, 1257
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 530, 1232, 1269
HpyF3I CTNAG 2 cut(s) 933, 1320
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 9 cut(s) 190, 218, 661, 718, 749, 943, 1184, 1394, 1406
Ksp22I TGATCA 3 cut(s) 1105, 1116, 1147
KspAI GTTAAC 1 cut(s) 925
LmnI GCTCC 2 cut(s) 47, 910
Lsp1109I GCAGC 5 cut(s) 344, 705, 753, 1269, 1272
LweI GCATC 2 cut(s) 511, 1075
MaeI CTAG 4 cut(s) 738, 1227, 1269, 1301
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 293, 1036
MflI RGATCY 2 cut(s) 360, 482
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 1214
MluCI AATT 3 cut(s) 242, 273, 842
MluNI TGGCCA 1 cut(s) 1214
MlyI GAGTC 3 cut(s) 551, 557, 937
Mox20I TGGCCA 1 cut(s) 1214
MroXI GAANNNNTTC 1 cut(s) 855
MscI TGGCCA 1 cut(s) 1214
MseI TTAA 8 cut(s) 588, 602, 645, 729, 798, 864, 924, 1196
MslI CAYNNNNRTG 1 cut(s) 321
Msp20I TGGCCA 1 cut(s) 1214
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 3 cut(s) 45, 81, 1200
MspR9I CCNGG 5 cut(s) 46, 564, 708, 1201, 1211
MvaI CCWGG 3 cut(s) 564, 708, 1211
MwoI GCNNNNNNNGC 4 cut(s) 118, 530, 1232, 1269
NciI CCSGG 2 cut(s) 46, 1201
NheI GCTAGC 2 cut(s) 737, 1226
NlaIII CATG 9 cut(s) 190, 218, 661, 718, 749, 943, 1184, 1394, 1406
NlaIV GGNNCC 2 cut(s) 1161, 1240
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 190
NspV TTCGAA 1 cut(s) 901
OliI CACNNNNGTG 1 cut(s) 321
PagI TCATGA 2 cut(s) 214, 939
PceI AGGCCT 1 cut(s) 1325
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 6 cut(s) 177, 425, 760, 880, 889, 1139
PflFI GACNNNGTC 1 cut(s) 299
PflMI CCANNNNNTGG 2 cut(s) 524, 1169
PkrI GCNGC 8 cut(s) 51, 79, 289, 334, 720, 743, 1259, 1262
PleI GAGTC 3 cut(s) 550, 556, 937
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 3 cut(s) 550, 556, 937
Ppu21I YACGTR 1 cut(s) 293
Psp6I CCWGG 3 cut(s) 562, 706, 1209
PspCI CACGTG 1 cut(s) 293
PspGI CCWGG 3 cut(s) 562, 706, 1209
PspN4I GGNNCC 2 cut(s) 1161, 1240
PspPI GGNCC 2 cut(s) 75, 1238
PstNI CAGNNNCTG 2 cut(s) 443, 524
PsuI RGATCY 2 cut(s) 360, 482
PsyI GACNNNGTC 1 cut(s) 299
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 8 cut(s) 588, 602, 645, 729, 798, 864, 924, 1196
SatI GCNGC 8 cut(s) 50, 78, 288, 333, 719, 742, 1258, 1261
Sau96I GGNCC 2 cut(s) 75, 1238
SchI GAGTC 3 cut(s) 551, 557, 937
ScrFI CCNGG 5 cut(s) 46, 564, 708, 1201, 1211
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 2 cut(s) 511, 1075
SfuI TTCGAA 1 cut(s) 901
SinI GGWCC 1 cut(s) 1238
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 1276
SmoI CTYRAG 1 cut(s) 1276
Sse9I AATT 3 cut(s) 242, 273, 842
SseBI AGGCCT 1 cut(s) 1325
SsiI CCGC 7 cut(s) 49, 78, 181, 287, 752, 908, 1285
SspMI CTAG 4 cut(s) 738, 1227, 1269, 1301
StuI AGGCCT 1 cut(s) 1325
StyD4I CCNGG 5 cut(s) 44, 562, 706, 1199, 1209
StyI CCWWGG 3 cut(s) 222, 1242, 1326
TaaI ACNGT 4 cut(s) 439, 784, 1006, 1315
TaiI ACGT 1 cut(s) 295
TaqI TCGA 5 cut(s) 145, 495, 639, 814, 901
TasI AATT 3 cut(s) 242, 273, 842
TatI WGTACW 1 cut(s) 685
TauI GCSGC 3 cut(s) 52, 80, 290
TfiI GAWTC 6 cut(s) 177, 425, 760, 880, 889, 1139
Tru1I TTAA 8 cut(s) 588, 602, 645, 729, 798, 864, 924, 1196
Tru9I TTAA 8 cut(s) 588, 602, 645, 729, 798, 864, 924, 1196
TscAI CASTG 2 cut(s) 1318, 1345
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 5 cut(s) 332, 718, 741, 1257, 1260
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 9 cut(s) 272, 402, 800, 823, 928, 1032, 1169, 1371, 1391
TspRI CASTG 2 cut(s) 1318, 1345
Tth111I GACNNNGTC 1 cut(s) 299
Van91I CCANNNNNTGG 2 cut(s) 524, 1169
VpaK11BI GGWCC 1 cut(s) 1238
XceI RCATGY 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 855
XspI CTAG 4 cut(s) 738, 1227, 1269, 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.