pycom01g18410
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001828
Physical Location & Seq
Forward (+)
17420264 .. 17422628
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18410.1

Sequence Viewer

Length: 1095 bp
ATGGAAGGGGGAAGGAGAATCTCTGTGAGTCCTCGGCCTTGTAACGGTAGGAGGGTGGTGGCGAAGAAACGACCTCGTATTGGTGGGGTTGATGGGTTCGTCAACAGCGTTAAGAAGCTTCAGAGGCGCGAAATCAGCTCCAAGCGCGACCGTGCTTTCACTATGAGCGATGCCCAAGAGAGGTTTCGCAATATTCGCCTCCAGGAGGAATATGATACACATGATCCGAAAGGTCATTGTGCCATGGTTCTGCCTTTCCTGCGAAAGAGGTCCAAAATTATTGAAATTGTAGCAGCACGTGACATTGTGTTTGCCCTTGCACAGTCTGGTGTTTGTGCAGCATTTAGCCGAGAAACCAACCAGAGAATATGCTTTCTGAATGTCAGTCCTGATGAAGTGATAAGAAGCTTGTTTTACAATAAAAACAATGACTCACTTATCACTGTCTCGGTTTATGCTTCAGACAACTTCAGTTCTTTGAAATGCAGAAGCACAAGGATTGAATACATACGAAGGGGTAAACCAGATGCTGGATTTGCTCTTTTTGAATCCGAGTCACTGAAATGGCCTGGTTTTGTAGAGTTCGATGATGTAAATGGGAAGGTTCTTACTTATTCTGCACAGGATAGTATATACAAGGTGTTTGATCTGAAAAACTATACAATGTTGTACTCCATATCGGATAAGCATGTCCAAGAGATTAAGATCAGTCCTGGGATCATGCTGTTGATTTTTACTAAAGCTAGCAGTCATGTTCCTCTTAAGATTCTGTCTATAGAGGATGGTACTGTTCTCAAATCCTTTAACCATCTCCTTCATCGGAATAAGAAGGTGGATTTCATTGAACAGTTCAACGAGAAGCTTCTTGTAAAGCAAGAAAATGAGAACCTTCAAATTCTTGATGTTCGCAATTTTGAGATGACAGAAGTAAGCAGAACTCAATTCATGACCCCATCAGCCTTCATATTTCTATATGAGAATCAGTTATTCTTGACGTTTCGGAATCGAACAGTGGCTGTTTGGAACTTTAGAGGAGAACTTGTGACTTCATTTGAGGATCGATCTTTTGTGGCATCCCGACTGCAATACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

365

Amino Acids

42.14

Weight (kDa)

9.56

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 51 - 357 1e-177 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 530
AccII CGCG 2 cut(s) 129, 147
AclWI GGATC 3 cut(s) 218, 725, 1065
AcsI RAATTY 1 cut(s) 894
AcuI CTGAAG 3 cut(s) 104, 444, 454
AcvI CACGTG 1 cut(s) 299
AfaI GTAC 2 cut(s) 671, 787
AfiI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 205, 530
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 7 cut(s) 284, 481, 503, 548, 845, 853, 893
AjnI CCWGG 3 cut(s) 201, 568, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 5 cut(s) 118, 138, 408, 743, 862
AluI AGCT 5 cut(s) 118, 138, 408, 743, 862
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 3 cut(s) 218, 725, 1065
AlwNI CAGNNNCTG 2 cut(s) 530, 1016
AoxI GGCC 2 cut(s) 35, 566
ApeKI GCWGC 2 cut(s) 293, 338
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspLEI GCGC 2 cut(s) 129, 147
AspS9I GGNCC 1 cut(s) 270
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 1 cut(s) 270
BbrPI CACGTG 1 cut(s) 299
BbvI GCAGC 2 cut(s) 305, 350
BccI CCATC 4 cut(s) 86, 776, 816, 961
BciT130I CCWGG 3 cut(s) 203, 570, 714
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 774
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 2 cut(s) 294, 339
BlsI GCNGC 2 cut(s) 295, 340
Bme1390I CCNGG 3 cut(s) 203, 570, 714
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 270
BmrFI CCNGG 3 cut(s) 203, 570, 714
BmsI GCATC 3 cut(s) 160, 517, 1082
BmtI GCTAGC 1 cut(s) 747
BpmI CTGGAG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 1060
BsaAI YACGTR 1 cut(s) 299
BsaBI GATNNNNATC 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 32, 243, 713
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 80, 180, 205, 530
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 3 cut(s) 203, 570, 714
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 3 cut(s) 32, 243, 713
BseGI GGATG 2 cut(s) 787, 1073
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 205, 530
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 2 cut(s) 305, 350
BsgI GTGCAG 2 cut(s) 357, 603
Bsh1236I CGCG 2 cut(s) 129, 147
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 2 cut(s) 37, 568
BshVI ATCGAT 1 cut(s) 1060
BsiEI CGRYCG 1 cut(s) 151
BslI CCNNNNNNNGG 5 cut(s) 44, 80, 180, 205, 530
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 2 cut(s) 37, 568
Bsp143I GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
Bsp19I CCATGG 1 cut(s) 243
BspANI GGCC 2 cut(s) 37, 568
BspDI ATCGAT 1 cut(s) 1060
BspFNI CGCG 2 cut(s) 129, 147
BspHI TCATGA 1 cut(s) 945
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 3 cut(s) 218, 725, 1065
BspTI CTTAAG 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 32, 243, 713
BssMI GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 3 cut(s) 203, 570, 714
Bst4CI ACNGT 7 cut(s) 47, 152, 324, 445, 790, 849, 1012
BstAFI CTTAAG 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 745
BstDSI CCRYGG 1 cut(s) 243
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 787, 1073
BstFNI CGCG 2 cut(s) 129, 147
BstHHI GCGC 2 cut(s) 129, 147
BstKTI GATC 6 cut(s) 226, 649, 708, 720, 1060, 1064
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
BstNI CCWGG 3 cut(s) 203, 570, 714
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 3 cut(s) 201, 568, 712
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 2 cut(s) 129, 147
BstV1I GCAGC 2 cut(s) 305, 350
Bsu15I ATCGAT 1 cut(s) 1060
BsuRI GGCC 2 cut(s) 37, 568
BsuTUI ATCGAT 1 cut(s) 1060
BtgI CCRYGG 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 787, 1073
BtsIMutI CAGTG 3 cut(s) 441, 557, 1017
Cac8I GCNNGC 1 cut(s) 745
CaiI CAGNNNCTG 2 cut(s) 530, 1016
CciI TCATGA 1 cut(s) 945
CfoI GCGC 2 cut(s) 129, 147
Cfr13I GGNCC 1 cut(s) 270
ClaI ATCGAT 1 cut(s) 1060
Csp6I GTAC 2 cut(s) 670, 786
CviAII CATG 6 cut(s) 221, 244, 689, 721, 752, 946
CviQI GTAC 2 cut(s) 670, 786
DpnI GATC 6 cut(s) 225, 648, 707, 719, 1059, 1063
DpnII GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 270
Eco57I CTGAAG 3 cut(s) 104, 444, 454
Eco72I CACGTG 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRII CCWGG 3 cut(s) 201, 568, 712
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 6 cut(s) 224, 247, 692, 724, 755, 949
FatI CATG 6 cut(s) 220, 243, 688, 720, 751, 945
Fnu4HI GCNGC 2 cut(s) 294, 339
FokI GGATG 2 cut(s) 794, 1060
Fsp4HI GCNGC 2 cut(s) 294, 339
FspBI CTAG 1 cut(s) 744
GlaI GCGC 2 cut(s) 128, 146
GluI GCNGC 2 cut(s) 294, 339
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 2 cut(s) 37, 568
HhaI GCGC 2 cut(s) 129, 147
Hin1II CATG 6 cut(s) 224, 247, 692, 724, 755, 949
Hin6I GCGC 2 cut(s) 127, 145
HinP1I GCGC 2 cut(s) 127, 145
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 3 cut(s) 116, 406, 860
HinfI GANTC 8 cut(s) 18, 28, 431, 548, 554, 766, 979, 1003
Hpy166II GTNNAC 2 cut(s) 103, 521
Hpy188I TCNGA 9 cut(s) 123, 228, 378, 463, 553, 651, 682, 822, 1002
Hpy188III TCNNGA 5 cut(s) 389, 899, 946, 991, 1077
Hpy8I GTNNAC 2 cut(s) 103, 521
HpyAV CCTTC 7 cut(s) 6, 507, 595, 823, 824, 899, 970
HpyCH4III ACNGT 7 cut(s) 47, 152, 324, 445, 790, 849, 1012
HpyCH4IV ACGT 2 cut(s) 298, 995
HpyCH4V TGCA 5 cut(s) 320, 338, 486, 620, 1084
HpyF10VI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
HpySE526I ACGT 2 cut(s) 298, 995
Hsp92II CATG 6 cut(s) 224, 247, 692, 724, 755, 949
HspAI GCGC 2 cut(s) 127, 145
Kzo9I GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 2 cut(s) 305, 350
LweI GCATC 3 cut(s) 160, 517, 1082
MaeI CTAG 1 cut(s) 744
MaeII ACGT 2 cut(s) 298, 995
MaeIII GTNAC 4 cut(s) 41, 299, 555, 1042
MalI GATC 6 cut(s) 225, 648, 707, 719, 1059, 1063
MboI GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
MboII GAAGA 1 cut(s) 76
MluCI AATT 5 cut(s) 276, 285, 894, 910, 941
MlyI GAGTC 3 cut(s) 37, 425, 563
MseI TTAA 4 cut(s) 111, 702, 762, 804
MslI CAYNNNNRTG 1 cut(s) 562
MspCI CTTAAG 1 cut(s) 761
MspR9I CCNGG 3 cut(s) 203, 570, 714
MvaI CCWGG 3 cut(s) 203, 570, 714
MvnI CGCG 2 cut(s) 129, 147
MwoI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
NcoI CCATGG 1 cut(s) 243
NdeII GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 6 cut(s) 224, 247, 692, 724, 755, 949
NmeAIII GCCGAG 2 cut(s) 13, 374
NmuCI GTSAC 3 cut(s) 299, 555, 1042
NspI RCATGY 1 cut(s) 692
PagI TCATGA 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 5 cut(s) 18, 548, 766, 979, 1003
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 2 cut(s) 295, 340
PleI GAGTC 3 cut(s) 36, 425, 562
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
PpsI GAGTC 3 cut(s) 36, 425, 562
Ppu21I YACGTR 1 cut(s) 299
Psp6I CCWGG 3 cut(s) 201, 568, 712
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 3 cut(s) 201, 568, 712
PspPI GGNCC 1 cut(s) 270
PstNI CAGNNNCTG 2 cut(s) 530, 1016
RsaI GTAC 2 cut(s) 671, 787
RsaNI GTAC 2 cut(s) 670, 786
RseI CAYNNNNRTG 1 cut(s) 562
SaqAI TTAA 4 cut(s) 111, 702, 762, 804
SatI GCNGC 2 cut(s) 294, 339
Sau3AI GATC 6 cut(s) 223, 646, 705, 717, 1057, 1061
Sau96I GGNCC 1 cut(s) 270
SchI GAGTC 3 cut(s) 37, 425, 563
ScrFI CCNGG 3 cut(s) 203, 570, 714
SfaNI GCATC 3 cut(s) 160, 517, 1082
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 562
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 5 cut(s) 276, 285, 894, 910, 941
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 201, 568, 712
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 7 cut(s) 47, 152, 324, 445, 790, 849, 1012
TaiI ACGT 2 cut(s) 301, 998
TaqI TCGA 3 cut(s) 585, 1006, 1060
TasI AATT 5 cut(s) 276, 285, 894, 910, 941
TatI WGTACW 1 cut(s) 669
TfiI GAWTC 5 cut(s) 18, 548, 766, 979, 1003
Tru1I TTAA 4 cut(s) 111, 702, 762, 804
Tru9I TTAA 4 cut(s) 111, 702, 762, 804
TscAI CASTG 3 cut(s) 448, 564, 1017
TseFI GTSAC 3 cut(s) 299, 555, 1042
TseI GCWGC 2 cut(s) 293, 338
Tsp45I GTSAC 3 cut(s) 299, 555, 1042
TspDTI ATGAA 6 cut(s) 408, 806, 829, 934, 952, 1038
TspRI CASTG 3 cut(s) 448, 564, 1017
Van91I CCANNNNNTGG 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 894
XceI RCATGY 1 cut(s) 692
XspI CTAG 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.