Rroxscaffold_4G00284650
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001828
Physical Location & Seq
Reverse (-)
6204772 .. 6208353
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284650.1

Sequence Viewer

Length: 1275 bp
ATGAGCGATGCCCAGGAGAGGTTTCGGAATATTCGTCTCCAGGAGGAATATGATACCCATGATCCAAAAGGTCATTGTGCCATGGTTCTGCCTTTTCTGCGAAAGAGGTCGAAAATTATTGAAATTGTAGCAGCACGTGACATTGTGTTTGCTCTTGCGCAGTCTGGTGTTTGTGCAGCGTTTAGCCGAGAAACCAATCAGAGGATATGCTTTCTGAATGTCAGTCCCGACGAAGTTATAAGAAGCTTGTTTTATAATAAAAACAATGACTCACTTATCACAGTTTCAGTTTATGCTTCAGACAATTTCAGTTCTTTGAAATGTAGAAGCACGAGGATTGAATACATACGAAGGGGTAAACCAGATGCTGGCTTTGCTCTTTTTGAGTCTGAGTCACTAAAGTGGCCTGGATTTGTAGAGTTCGATGATGTAAATGGGAAGGTTCTCACTTACTCTGCACAAGATAGTATATACAAGGTGTTTGATCTTAAAAACTATACAATGTTGTACTCCATATCAGATAAACATGTCCAAGAAATAAAGATTAGTCCGGGGATCATGTTGTTAATTTTCAATAGAGCTAGTAGCCATGTTCCTCTCAAGATTCTTTCTATAGAGGATGGTAGTGTTCTCAAATCCTTCAACCATCTACTTCATCGGAATAAGAAGGTAGATTTCATTGAACAGTTCAATGAAAAGCTTCTTGTAAAACAAGAAAATGAGAACCTCCAGATTCTTGATGTTCGCAATTCTGAGATGACAGAAGTTAGCAAAAGTGAATTCATGACACCATCGGCTTTTATATTTCTGTACGAGAATCAGTTATTTTTAACGTTTCGTAATCGTACTGTGGCTGTTTGGAACTTTCGAGGGGAACTTGTGACTTCGTTTGAGGATCATCTTTTGTGGCATCCCGACTGCAATACTAATAACATATATATTACTAGTGACCAGGATCTTATTATTTCTTACTGCAAGGCTGATTCTGATGATCCATTAGCTGAAGGAAATGCAGGATCCATCAATATCAGCAATATTTTGACAGGGAAATGCCTTGCTAAAATAAAAGCCAGCAAGAATGTGGAAAATGAATGCAGTTGTAGTGGTGACTGTTGTGGAAGCAGTTGCAGTTCTAAGAAGCGTGCCCAGTTATCCAGAATTAGAAGTACAGTCGCAGAAGCCTTGGAAGATATTACTGCTCTCTTCTACGATGAAGAGCGCAACGAGATCTATACAGGCAATAGGCTCGGTCTAGTTCATGTATGGTCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

48.33

Weight (kDa)

5.93

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 3 - 422 1.2e-226 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 239, 255
Acc16I TGCGCA 1 cut(s) 159
AccB7I CCANNNNNTGG 1 cut(s) 368
AclI AACGTT 1 cut(s) 833
AclWI GGATC 7 cut(s) 56, 563, 903, 963, 986, 1011, 1024
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 2 cut(s) 282, 1023
AcvI CACGTG 1 cut(s) 137
AfaI GTAC 4 cut(s) 509, 812, 847, 1168
AfiI CCNNNNNNNGG 3 cut(s) 18, 201, 368
AflIII ACRYGT 1 cut(s) 526
AgsI TTSAA 7 cut(s) 122, 319, 341, 574, 643, 683, 691
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 4 cut(s) 12, 39, 406, 951
AleI CACNNNNGTG 1 cut(s) 400
AluBI AGCT 4 cut(s) 246, 581, 700, 1001
AluI AGCT 4 cut(s) 246, 581, 700, 1001
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 7 cut(s) 56, 563, 903, 963, 986, 1011, 1024
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 131, 176
ApoI RAATTY 1 cut(s) 779
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 1 cut(s) 699
AspLEI GCGC 2 cut(s) 160, 1221
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 1 cut(s) 1118
BaeGI GKGCMC 1 cut(s) 1147
BamHI GGATCC 1 cut(s) 1016
BauI CACGAG 1 cut(s) 331
BbrPI CACGTG 1 cut(s) 137
BbvI GCAGC 2 cut(s) 143, 188
BccI CCATC 4 cut(s) 614, 654, 799, 1028
BcgI CGANNNNNNTGC 2 cut(s) 1228, 1262
BciT130I CCWGG 4 cut(s) 14, 41, 408, 953
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 1 cut(s) 41
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 3 cut(s) 582, 945, 1253
BfmI CTRYAG 1 cut(s) 612
BglII AGATCT 1 cut(s) 1227
BisI GCNGC 2 cut(s) 132, 177
BlsI GCNGC 2 cut(s) 133, 178
Bme1390I CCNGG 5 cut(s) 14, 41, 408, 552, 953
BmiI GGNNCC 1 cut(s) 1018
BmrFI CCNGG 5 cut(s) 14, 41, 408, 552, 953
BmrI ACTGGG 1 cut(s) 1141
BmsI GCATC 2 cut(s) 355, 919
BmuI ACTGGG 1 cut(s) 1141
BpmI CTGGAG 2 cut(s) 23, 713
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 137
BsaJI CCNNGG 4 cut(s) 12, 81, 551, 1182
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 201, 368
Bse1I ACTGG 1 cut(s) 1147
BseBI CCWGG 4 cut(s) 14, 41, 408, 953
BseDI CCNNGG 4 cut(s) 12, 81, 551, 1182
BseGI GGATG 2 cut(s) 625, 910
BseLI CCNNNNNNNGG 3 cut(s) 18, 201, 368
BseMII CTCAG 2 cut(s) 381, 744
BseNI ACTGG 1 cut(s) 1147
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 2 cut(s) 143, 188
BsgI GTGCAG 2 cut(s) 195, 441
BshFI GGCC 1 cut(s) 406
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 3 cut(s) 18, 201, 368
BsmAI GTCTC 1 cut(s) 41
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 1147
Bsp143I GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
Bsp19I CCATGG 1 cut(s) 81
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 2 cut(s) 382, 745
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 1 cut(s) 1018
BspPI GGATC 7 cut(s) 56, 563, 903, 963, 986, 1011, 1024
BspQI GCTCTTC 1 cut(s) 1209
BsrI ACTGG 1 cut(s) 1147
BssECI CCNNGG 4 cut(s) 12, 81, 551, 1182
BssMI GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
BssSI CACGAG 1 cut(s) 331
BssT1I CCWWGG 2 cut(s) 81, 1182
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 4 cut(s) 14, 41, 408, 953
Bst4CI ACNGT 5 cut(s) 283, 687, 850, 1112, 1171
Bst6I CTCTTC 2 cut(s) 1208, 1209
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 370, 1072, 1143
BstDEI CTNAG 3 cut(s) 390, 753, 1134
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 625, 910
BstHHI GCGC 2 cut(s) 160, 1221
BstKTI GATC 8 cut(s) 64, 487, 558, 898, 958, 994, 1019, 1230
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
BstMWI GCNNNNNNNGC 2 cut(s) 97, 374
BstNI CCWGG 4 cut(s) 14, 41, 408, 953
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 12, 39, 406, 550, 951
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 1147
BstV1I GCAGC 2 cut(s) 143, 188
BstX2I RGATCY 3 cut(s) 955, 1016, 1227
BstYI RGATCY 3 cut(s) 955, 1016, 1227
BsuRI GGCC 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 2 cut(s) 625, 910
Cac8I GCNNGC 3 cut(s) 370, 1072, 1143
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 1 cut(s) 783
CfoI GCGC 2 cut(s) 160, 1221
Csp6I GTAC 4 cut(s) 508, 811, 846, 1167
CviAII CATG 7 cut(s) 59, 82, 527, 559, 590, 784, 1259
CviQI GTAC 4 cut(s) 508, 811, 846, 1167
DdeI CTNAG 3 cut(s) 390, 753, 1134
DpnI GATC 8 cut(s) 63, 486, 557, 897, 957, 993, 1018, 1229
DpnII GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
Eam1104I CTCTTC 2 cut(s) 1208, 1209
EarI CTCTTC 2 cut(s) 1208, 1209
Eco130I CCWWGG 2 cut(s) 81, 1182
Eco57I CTGAAG 2 cut(s) 282, 1023
Eco72I CACGTG 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 779
EcoRII CCWGG 4 cut(s) 12, 39, 406, 951
EcoT14I CCWWGG 2 cut(s) 81, 1182
ErhI CCWWGG 2 cut(s) 81, 1182
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 7 cut(s) 62, 85, 530, 562, 593, 787, 1262
FaqI GGGAC 1 cut(s) 210
FatI CATG 7 cut(s) 58, 81, 526, 558, 589, 783, 1258
Fnu4HI GCNGC 2 cut(s) 132, 177
FokI GGATG 2 cut(s) 632, 897
Fsp4HI GCNGC 2 cut(s) 132, 177
FspBI CTAG 3 cut(s) 582, 945, 1253
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 2 cut(s) 159, 1220
GluI GCNGC 2 cut(s) 132, 177
GsuI CTGGAG 2 cut(s) 23, 713
HaeIII GGCC 1 cut(s) 406
HapII CCGG 1 cut(s) 551
HhaI GCGC 2 cut(s) 160, 1221
Hin1II CATG 7 cut(s) 62, 85, 530, 562, 593, 787, 1262
Hin6I GCGC 2 cut(s) 158, 1219
HinP1I GCGC 2 cut(s) 158, 1219
HindIII AAGCTT 2 cut(s) 244, 698
HinfI GANTC 7 cut(s) 269, 386, 392, 604, 733, 817, 983
HpaII CCGG 1 cut(s) 551
HphI GGTGA 1 cut(s) 1118
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 9 cut(s) 27, 201, 216, 301, 391, 520, 660, 754, 988
Hpy188III TCNNGA 7 cut(s) 227, 601, 730, 737, 784, 914, 1155
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 5 cut(s) 345, 433, 649, 661, 998
HpyCH4III ACNGT 5 cut(s) 283, 687, 850, 1112, 1171
HpyCH4IV ACGT 2 cut(s) 136, 833
HpyCH4V TGCA 7 cut(s) 176, 458, 921, 975, 1013, 1095, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 374
HpyF3I CTNAG 3 cut(s) 390, 753, 1134
HpySE526I ACGT 2 cut(s) 136, 833
Hsp92II CATG 7 cut(s) 62, 85, 530, 562, 593, 787, 1262
HspAI GCGC 2 cut(s) 158, 1219
Kzo9I GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
LguI GCTCTTC 1 cut(s) 1209
Lsp1109I GCAGC 2 cut(s) 143, 188
LweI GCATC 2 cut(s) 355, 919
MaeI CTAG 3 cut(s) 582, 945, 1253
MaeII ACGT 2 cut(s) 136, 833
MaeIII GTNAC 5 cut(s) 137, 393, 880, 947, 1106
MalI GATC 8 cut(s) 63, 486, 557, 897, 957, 993, 1018, 1229
MboI GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
MboII GAAGA 3 cut(s) 1195, 1199, 1226
MflI RGATCY 3 cut(s) 955, 1016, 1227
MhlI GDGCHC 1 cut(s) 1147
MluCI AATT 7 cut(s) 114, 123, 304, 567, 748, 779, 1158
MlyI GAGTC 3 cut(s) 263, 395, 401
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 3 cut(s) 489, 566, 830
MslI CAYNNNNRTG 1 cut(s) 400
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 5 cut(s) 14, 41, 408, 552, 953
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 4 cut(s) 14, 41, 408, 953
MwoI GCNNNNNNNGC 2 cut(s) 97, 374
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 1 cut(s) 81
NdeII GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
NlaIII CATG 7 cut(s) 62, 85, 530, 562, 593, 787, 1262
NlaIV GGNNCC 1 cut(s) 1018
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 5 cut(s) 137, 393, 880, 947, 1106
NsbI TGCGCA 1 cut(s) 159
NspI RCATGY 1 cut(s) 530
OliI CACNNNNGTG 1 cut(s) 400
PagI TCATGA 1 cut(s) 783
PciI ACATGT 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 1209
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PctI GAATGC 1 cut(s) 1097
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 4 cut(s) 604, 733, 817, 983
PflMI CCANNNNNTGG 1 cut(s) 368
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 2 cut(s) 133, 178
PleI GAGTC 3 cut(s) 263, 394, 400
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 3 cut(s) 263, 394, 400
Ppu21I YACGTR 1 cut(s) 137
PscI ACATGT 1 cut(s) 526
PsiI TTATAA 2 cut(s) 239, 255
Psp1406I AACGTT 1 cut(s) 833
Psp6I CCWGG 4 cut(s) 12, 39, 406, 951
PspCI CACGTG 1 cut(s) 137
PspGI CCWGG 4 cut(s) 12, 39, 406, 951
PspN4I GGNNCC 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 3 cut(s) 955, 1016, 1227
RsaI GTAC 4 cut(s) 509, 812, 847, 1168
RsaNI GTAC 4 cut(s) 508, 811, 846, 1167
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 1209
SaqAI TTAA 3 cut(s) 489, 566, 830
SatI GCNGC 2 cut(s) 132, 177
Sau3AI GATC 8 cut(s) 61, 484, 555, 895, 955, 991, 1016, 1227
SchI GAGTC 3 cut(s) 263, 395, 401
ScrFI CCNGG 5 cut(s) 14, 41, 408, 552, 953
SduI GDGCHC 1 cut(s) 1147
SfaNI GCATC 2 cut(s) 355, 919
SfcI CTRYAG 1 cut(s) 612
SmiMI CAYNNNNRTG 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SpeI ACTAGT 1 cut(s) 944
Sse9I AATT 7 cut(s) 114, 123, 304, 567, 748, 779, 1158
SspI AATATT 2 cut(s) 31, 1036
SspMI CTAG 3 cut(s) 582, 945, 1253
StyD4I CCNGG 5 cut(s) 12, 39, 406, 550, 951
StyI CCWWGG 2 cut(s) 81, 1182
TaaI ACNGT 5 cut(s) 283, 687, 850, 1112, 1171
TaiI ACGT 2 cut(s) 139, 836
TaqI TCGA 3 cut(s) 110, 423, 868
TaqII GACCGA 1 cut(s) 1238
TasI AATT 7 cut(s) 114, 123, 304, 567, 748, 779, 1158
TatI WGTACW 2 cut(s) 507, 1166
TfiI GAWTC 4 cut(s) 604, 733, 817, 983
Tru1I TTAA 3 cut(s) 489, 566, 830
Tru9I TTAA 3 cut(s) 489, 566, 830
TseFI GTSAC 5 cut(s) 137, 393, 880, 947, 1106
TseI GCWGC 2 cut(s) 131, 176
Tsp45I GTSAC 5 cut(s) 137, 393, 880, 947, 1106
TspDTI ATGAA 7 cut(s) 644, 667, 708, 772, 1104, 1227, 1247
Van91I CCANNNNNTGG 1 cut(s) 368
XapI RAATTY 1 cut(s) 779
XceI RCATGY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 3 cut(s) 582, 945, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.