MD04G1179700.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001828
Physical Location & Seq
Forward (+)
27078008 .. 27082099
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1179700.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGGAAGTGGGAAGGAGGATATCGGCGAGTCCTCGACCGTGCTCGGGGCGGCGAGTGGTGGCGAAGAAGAGGAGGGGTGGTTCCGATGGGTTCGTTAACAGCGTGAAGAAGCTTCAGAGGCGTGAGATTTCTTCGAAGCGTGATCGGGCTTTTGGGATGAGCAATTCCCAGGAGAGGTTCCGCAACATGCACCTCGTGGAGCAATATGATACTCATGATCCCAAGGGGCATTCTTCGGCCATATTGCCTTTTCTGATGAAAAGGACAAAGGTCATTGAGATAGTTTCTGCACGTGACATCGTTTTTGCTCTTGCACACTCTGGTGTTTGCGCAGCTTTTAGTAGAGAGAAAAACGAGAGGATTTGTTTCATGAATGTTAGTCCTGATGAAGTCATTCGAAGCTTGTTCTATAATAAGAACAATGATTCACTCATCACAGTTTCGGTGTATGCTTCAGACAACTTCAGCTCTTTGAAATGCAGATCCACAAGGATTGAATATATAAGGAGGGGACAGCCAGAAGCTGGTTTTTCTCTTTTCGAGTCTGAGTCTTTGAAGTGGCCTGGGTTTGTAGAGTTTGATGATGTTAATGGCAAGGTGCTGACATACTCAGCACAAGATAGTATATACAAGGTGTTTGATCTGAAAAACTATACCATGCTGTACTCTATATCAGACAAACATGTCCAAGAAATCAAAATCAGTCCAGGCATCATGCTGTTGATTTATAACAGAGCAAGTAGCCATGTTCCTCTGAAAATTCTGTCAATAGAAGATGGCACAGTTCTCAAAGACTTCAATCATCTACTTCATAGAAATAAGAAGGTGGACTTCATAGAACAGTTCAATGAAAAGCTTCTTGTCAAGCAAGAAAATGAGAATCTTCAGATTCTGGATGTTCGAAATGCTGAGCTGATGGAAGTTAGCAGAACAGACTTCATCACTCCATCAGCATTTATCTTTCTGTATGAGAACCAGCTATTCCTGACATTCAGAAATCGATTGGTGTCTGTCTGGAACTTTCGTGGAGAGCTTGTCACTGCATTTGAGGATCACTTGTTATGGCATCCTGACTGCAATACCAATAACATTTATATAACAAGCGATCAGGATCTTATCATATCTTACTGCAAGGCCGATTCTGATGATCATTGGTCGGAAGGAAATGCTGGATCTATTCATGTTAGCAATATCTTAACCGGAAAATGCCTGGCCAAAATAAATCCGAGCAATGGTGATCCCAAGGTCGATGACACAGAAGGCAACACTGGCTCCTCAAGGAAGCTAAGCAACTCATCTCTGGTGAGAAGCACAGTCGCTGAGGCTTTGGAAGACGTAACTGCTCTCTTCTATGATGAAGATCGCAATGAGATATATACCGGAAATAGGCATGGTCTTGTTCATGTGTGGTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

473

Amino Acids

53.88

Weight (kDa)

7.23

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 49 - 470 3.5e-224 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AasI GACNNNNNNGTC 1 cut(s) 683
Acc16I TGCGCA 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 524
AciI CCGC 2 cut(s) 49, 181
AclWI GGATC 6 cut(s) 212, 477, 1059, 1119, 1180, 1232
AcoI YGGCCR 2 cut(s) 237, 1212
AcsI RAATTY 1 cut(s) 759
AcuI CTGAAG 4 cut(s) 98, 438, 448, 869
AcvI CACGTG 1 cut(s) 293
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 1 cut(s) 665
AfiI CCNNNNNNNGG 5 cut(s) 44, 174, 524, 1232, 1386
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 5 cut(s) 475, 497, 556, 799, 847
AjnI CCWGG 4 cut(s) 168, 562, 706, 1209
AleI CACNNNNGTG 1 cut(s) 321
AloI GAACNNNNNNTCC 2 cut(s) 64, 96
Alw21I GWGCWC 1 cut(s) 44
AlwI GGATC 6 cut(s) 212, 477, 1059, 1119, 1180, 1232
AlwNI CAGNNNCTG 3 cut(s) 524, 892, 1319
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 4 cut(s) 237, 560, 1134, 1212
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 759
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 2 cut(s) 393, 855
AspLEI GCGC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 1247, 1315
AsuII TTCGAA 3 cut(s) 134, 397, 901
AvaI CYCGRG 1 cut(s) 43
BalI TGGCCA 1 cut(s) 1214
BauI CACGAG 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 1338
Bbv12I GWGCWC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 1320
BbvI GCAGC 1 cut(s) 344
BccI CCATC 4 cut(s) 80, 770, 910, 955
BciT130I CCWGG 4 cut(s) 170, 564, 708, 1211
BclI TGATCA 1 cut(s) 1147
BisI GCNGC 2 cut(s) 50, 333
BlpI GCTNAGC 2 cut(s) 909, 1286
BlsI GCNGC 2 cut(s) 51, 334
Bme1390I CCNGG 4 cut(s) 170, 564, 708, 1211
BmeT110I CYCGRG 1 cut(s) 43
BmiI GGNNCC 3 cut(s) 82, 179, 1273
BmrFI CCNGG 4 cut(s) 170, 564, 708, 1211
BmsI GCATC 2 cut(s) 720, 1075
BoxI GACNNNNGTC 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 1338
Bpu10I CCTNAGC 1 cut(s) 1320
Bpu1102I GCTNAGC 2 cut(s) 909, 1286
Bpu14I TTCGAA 3 cut(s) 134, 397, 901
BpuEI CTTGAG 1 cut(s) 1261
Bsa29I ATCGAT 1 cut(s) 1000
BsaAI YACGTR 1 cut(s) 293
BsaBI GATNNNNATC 2 cut(s) 1110, 1359
BsaJI CCNNGG 4 cut(s) 168, 222, 563, 1242
BsaWI WCCGGW 2 cut(s) 1199, 1379
BsaXI ACNNNNNCTCC 6 cut(s) 64, 94, 1020, 1050, 1256, 1286
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 174, 524, 1232, 1386
Bse1I ACTGG 1 cut(s) 1273
Bse3DI GCAATG 2 cut(s) 1237, 1372
Bse8I GATNNNNATC 2 cut(s) 1110, 1359
BseBI CCWGG 4 cut(s) 170, 564, 708, 1211
BseCI ATCGAT 1 cut(s) 1000
BseDI CCNNGG 4 cut(s) 168, 222, 563, 1242
BseGI GGATG 3 cut(s) 162, 901, 1066
BseJI GATNNNNATC 2 cut(s) 1110, 1359
BseLI CCNNNNNNNGG 5 cut(s) 44, 174, 524, 1232, 1386
BseMI GCAATG 2 cut(s) 1237, 1372
BseMII CTCAG 4 cut(s) 537, 624, 900, 1311
BseNI ACTGG 1 cut(s) 1273
BseRI GAGGAG 2 cut(s) 85, 1264
BseXI GCAGC 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 4 cut(s) 239, 562, 1136, 1214
BshVI ATCGAT 1 cut(s) 1000
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 1200, 1380
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 5 cut(s) 44, 174, 524, 1232, 1386
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 4 cut(s) 239, 562, 1136, 1214
BsoBI CYCGRG 1 cut(s) 43
Bsp119I TTCGAA 3 cut(s) 134, 397, 901
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1720I GCTNAGC 2 cut(s) 909, 1286
BspACI CCGC 2 cut(s) 49, 181
BspANI GGCC 4 cut(s) 239, 562, 1136, 1214
BspCNI CTCAG 4 cut(s) 538, 623, 901, 1312
BspDI ATCGAT 1 cut(s) 1000
BspHI TCATGA 2 cut(s) 214, 369
BspLI GGNNCC 3 cut(s) 82, 179, 1273
BspPI GGATC 6 cut(s) 212, 477, 1059, 1119, 1180, 1232
BspT104I TTCGAA 3 cut(s) 134, 397, 901
BsrDI GCAATG 2 cut(s) 1237, 1372
BsrI ACTGG 1 cut(s) 1273
BssECI CCNNGG 4 cut(s) 168, 222, 563, 1242
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 2 cut(s) 222, 1242
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 4 cut(s) 170, 564, 708, 1211
Bst4CI ACNGT 5 cut(s) 39, 439, 784, 843, 1315
Bst6I CTCTTC 2 cut(s) 62, 1352
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 3 cut(s) 134, 397, 901
BstDEI CTNAG 5 cut(s) 546, 610, 909, 1286, 1320
BstF5I GGATG 3 cut(s) 162, 901, 1066
BstHHI GCGC 1 cut(s) 332
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 118, 1269
BstNI CCWGG 4 cut(s) 170, 564, 708, 1211
BstNSI RCATGY 2 cut(s) 190, 686
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 4 cut(s) 168, 562, 706, 1209
BstV1I GCAGC 1 cut(s) 344
BstV2I GAAGAC 1 cut(s) 1338
BstX2I RGATCY 3 cut(s) 482, 1111, 1172
BstYI RGATCY 3 cut(s) 482, 1111, 1172
Bsu15I ATCGAT 1 cut(s) 1000
BsuRI GGCC 4 cut(s) 239, 562, 1136, 1214
BsuTUI ATCGAT 1 cut(s) 1000
BtsCI GGATG 3 cut(s) 162, 901, 1066
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 2 cut(s) 1038, 1266
CaiI CAGNNNCTG 3 cut(s) 524, 892, 1319
CciI TCATGA 2 cut(s) 214, 369
CfoI GCGC 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 1000
Csp6I GTAC 1 cut(s) 664
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 5 cut(s) 546, 610, 909, 1286, 1320
DraIII CACNNNGTG 1 cut(s) 196
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 237, 1212
Eam1104I CTCTTC 2 cut(s) 62, 1352
EarI CTCTTC 2 cut(s) 62, 1352
Eco130I CCWWGG 2 cut(s) 222, 1242
Eco32I GATATC 1 cut(s) 21
Eco57I CTGAAG 4 cut(s) 98, 438, 448, 869
Eco72I CACGTG 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 4 cut(s) 168, 562, 706, 1209
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 222, 1242
ErhI CCWWGG 2 cut(s) 222, 1242
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 525
FbaI TGATCA 1 cut(s) 1147
Fnu4HI GCNGC 2 cut(s) 50, 333
FokI GGATG 3 cut(s) 169, 908, 1053
Fsp4HI GCNGC 2 cut(s) 50, 333
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 50, 333
HaeIII GGCC 4 cut(s) 239, 562, 1136, 1214
HapII CCGG 2 cut(s) 1200, 1380
HhaI GCGC 1 cut(s) 332
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 3 cut(s) 110, 400, 854
HinfI GANTC 7 cut(s) 28, 425, 542, 548, 880, 889, 1139
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 2 cut(s) 1200, 1380
HphI GGTGA 2 cut(s) 1247, 1315
Hpy166II GTNNAC 2 cut(s) 97, 829
Hpy188III TCNNGA 8 cut(s) 215, 370, 383, 893, 985, 1015, 1070, 1109
Hpy8I GTNNAC 2 cut(s) 97, 829
HpyAV CCTTC 4 cut(s) 6, 817, 1154, 1253
HpyCH4III ACNGT 5 cut(s) 39, 439, 784, 843, 1315
HpyCH4IV ACGT 2 cut(s) 292, 1335
HpyCH4V TGCA 7 cut(s) 190, 290, 314, 480, 1043, 1077, 1131
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 1269
HpyF3I CTNAG 5 cut(s) 546, 610, 909, 1286, 1320
HpySE526I ACGT 2 cut(s) 292, 1335
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 1147
KspAI GTTAAC 1 cut(s) 97
LmnI GCTCC 2 cut(s) 199, 1277
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 2 cut(s) 720, 1075
MaeII ACGT 2 cut(s) 292, 1335
MaeIII GTNAC 3 cut(s) 293, 1036, 1336
MflI RGATCY 3 cut(s) 482, 1111, 1172
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 1214
MluCI AATT 2 cut(s) 163, 759
MluNI TGGCCA 1 cut(s) 1214
MlyI GAGTC 3 cut(s) 37, 551, 557
MmeI TCCRAC 1 cut(s) 1137
Mox20I TGGCCA 1 cut(s) 1214
MroXI GAANNNNTTC 2 cut(s) 393, 855
MscI TGGCCA 1 cut(s) 1214
MseI TTAA 3 cut(s) 96, 588, 1196
MslI CAYNNNNRTG 1 cut(s) 321
Msp20I TGGCCA 1 cut(s) 1214
MspI CCGG 2 cut(s) 1200, 1380
MspR9I CCNGG 4 cut(s) 170, 564, 708, 1211
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 4 cut(s) 170, 564, 708, 1211
MwoI GCNNNNNNNGC 2 cut(s) 118, 1269
NlaIV GGNNCC 3 cut(s) 82, 179, 1273
NmuCI GTSAC 2 cut(s) 293, 1036
NsbI TGCGCA 1 cut(s) 331
NspI RCATGY 2 cut(s) 190, 686
NspV TTCGAA 3 cut(s) 134, 397, 901
OliI CACNNNNGTG 1 cut(s) 321
PagI TCATGA 2 cut(s) 214, 369
PciI ACATGT 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 2 cut(s) 393, 855
PfeI GAWTC 4 cut(s) 425, 880, 889, 1139
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 2 cut(s) 51, 334
PleI GAGTC 3 cut(s) 36, 550, 556
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 3 cut(s) 36, 550, 556
Ppu21I YACGTR 1 cut(s) 293
PscI ACATGT 1 cut(s) 682
PshAI GACNNNNGTC 1 cut(s) 269
PsiI TTATAA 1 cut(s) 729
Psp6I CCWGG 4 cut(s) 168, 562, 706, 1209
PspCI CACGTG 1 cut(s) 293
PspGI CCWGG 4 cut(s) 168, 562, 706, 1209
PspN4I GGNNCC 3 cut(s) 82, 179, 1273
PstNI CAGNNNCTG 3 cut(s) 524, 892, 1319
PsuI RGATCY 3 cut(s) 482, 1111, 1172
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 3 cut(s) 96, 588, 1196
SatI GCNGC 2 cut(s) 50, 333
SchI GAGTC 3 cut(s) 37, 551, 557
ScrFI CCNGG 4 cut(s) 170, 564, 708, 1211
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 2 cut(s) 720, 1075
SfuI TTCGAA 3 cut(s) 134, 397, 901
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 1276
SmoI CTYRAG 1 cut(s) 1276
Sse9I AATT 2 cut(s) 163, 759
SsiI CCGC 2 cut(s) 49, 181
StyD4I CCNGG 4 cut(s) 168, 562, 706, 1209
StyI CCWWGG 2 cut(s) 222, 1242
TaaI ACNGT 5 cut(s) 39, 439, 784, 843, 1315
TaiI ACGT 2 cut(s) 295, 1338
TaqI TCGA 7 cut(s) 34, 134, 397, 540, 901, 1000, 1248
TasI AATT 2 cut(s) 163, 759
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 4 cut(s) 425, 880, 889, 1139
Tru1I TTAA 3 cut(s) 96, 588, 1196
Tru9I TTAA 3 cut(s) 96, 588, 1196
TscAI CASTG 2 cut(s) 1045, 1273
TseFI GTSAC 2 cut(s) 293, 1036
TseI GCWGC 1 cut(s) 332
Tsp45I GTSAC 2 cut(s) 293, 1036
TspRI CASTG 2 cut(s) 1045, 1273
Van91I CCANNNNNTGG 1 cut(s) 524
XapI RAATTY 1 cut(s) 759
XceI RCATGY 2 cut(s) 190, 686
XmnI GAANNNNTTC 2 cut(s) 393, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.