Rorug03G0036500
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001828
Physical Location & Seq
Reverse (-)
2921687 .. 2924686
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0036500.1

Sequence Viewer

Length: 942 bp
ATGGCTTTGAGAAATCCGGCCACCGTCGCCGCGTTATTGCTTGCTCTTCTATTGATTTCCGACCCCAATTTCGCGTCCGCCGCTACGGGTGGGCGGTCCGGCGGAAGCCTGTTCCCCTCCGGCGGAGATTCTTCTTCATCTTCATCTTCGTCTTCATCGTGGTCCGGGTCTTCGGGTTCATCGGACTCTTGGTCGTGGTACCACTCGCCGTCGTCGTCGTCGTCTTCCTCGTCGGACGGTGGACTCTACGTCTTCTTGGTTATAATGGCGGTTGTGGTTGGTGTGGCGGTCATCTGCCGCTGTGCTGGTGTTGGGAAAACAAGCGTCGTCAAGCTTCAGGTTGGTCTGGTGGGCAAGGCGCGATCACTTCAAAAGGATCTTAATCGACTTACTGAAACTACAGATATGTCTACGTCTACGGGTTTGAGCCATCTGTTGACAGGGGCATCAACATCTTTGCTTCGCAATCTTGATTATTGCATCTCAAGTTATTCATCTGTTACTCGAAAGTCCGAGACAGAAGATGCAGAGCAATGCTTCAAACAACTTTCTATGGAAGAAGCAGCTAAATTTGATGAAGTGACGCTTGCCAATGTGAACAACTTTATAGTGCGTTCAACAAGCCCGACACCAAAAGAAGTCCACAAGGAATATATAGTGGTTACAATTTTGGTGGCTGCTCAAGGAGCACCTAAGTTGCCTAGCATCAATGGCCCAGGTGACTTGAAGAAAGCATTACAGAGTCTTGGATCCATTCCCTCGAACAAAATACTGGCGGTTGAGGTCTTGTGGACTCCTCAACAAGAAAATGATACATTGACTGAGAAGGAACTACTTAAAGATTACCCGCAGTTGAGGGGCTTTTATGATGAAGGGTCACTTGAAGAAGGGCCATATGACGATTCCCACCCGTTCTTAGGGGTTACTGTAGTCAAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

33.06

Weight (kDa)

5.31

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 107 - 286 1.7e-60 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 198
AccI GTMKAC 2 cut(s) 410, 416
AccII CGCG 3 cut(s) 32, 74, 361
AclWI GGATC 3 cut(s) 384, 744, 757
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 122, 917
AgsI TTSAA 5 cut(s) 371, 541, 618, 727, 884
AhdI GACNNNNNGTC 2 cut(s) 190, 248
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 2 cut(s) 334, 566
AluI AGCT 2 cut(s) 334, 566
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 1 cut(s) 509
AlwI GGATC 3 cut(s) 384, 744, 757
AoxI GGCC 3 cut(s) 18, 712, 890
ApeKI GCWGC 2 cut(s) 563, 677
ApoI RAATTY 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp718I GGTACC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 4 cut(s) 96, 162, 713, 890
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 731
AvaII GGWCC 2 cut(s) 96, 162
BamHI GGATCC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 198
BbsI GAAGAC 4 cut(s) 144, 162, 216, 244
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 2 cut(s) 575, 664
BccI CCATC 1 cut(s) 438
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 717
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 509
BfaI CTAG 2 cut(s) 702, 940
BfmI CTRYAG 2 cut(s) 399, 927
BisI GCNGC 5 cut(s) 30, 81, 298, 564, 678
BlsI GCNGC 5 cut(s) 31, 82, 299, 565, 679
Bme1390I CCNGG 2 cut(s) 166, 717
Bme18I GGWCC 2 cut(s) 96, 162
BmeRI GACNNNNNGTC 2 cut(s) 190, 248
BmgT120I GGNCC 4 cut(s) 96, 162, 713, 890
BmiI GGNNCC 2 cut(s) 200, 751
BmrFI CCNGG 2 cut(s) 166, 717
BmsI GCATC 4 cut(s) 455, 489, 514, 714
BpiI GAAGAC 4 cut(s) 144, 162, 216, 244
BpuEI CTTGAG 2 cut(s) 469, 666
BpuMI CCSGG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 715
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 917
Bse1I ACTGG 1 cut(s) 777
Bse3DI GCAATG 1 cut(s) 539
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 2 cut(s) 122, 917
BseMI GCAATG 1 cut(s) 539
BseMII CTCAG 1 cut(s) 813
BseNI ACTGG 1 cut(s) 777
BseRI GAGGAG 1 cut(s) 786
BseXI GCAGC 2 cut(s) 575, 664
Bsh1236I CGCG 3 cut(s) 32, 74, 361
BshFI GGCC 3 cut(s) 20, 714, 892
BshNI GGYRCC 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 4 cut(s) 17, 99, 120, 165
BslI CCNNNNNNNGG 2 cut(s) 122, 917
BsmAI GTCTC 1 cut(s) 509
BsnI GGCC 3 cut(s) 20, 714, 892
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 3 cut(s) 362, 376, 749
BspANI GGCC 3 cut(s) 20, 714, 892
BspCNI CTCAG 1 cut(s) 814
BspFNI CGCG 3 cut(s) 32, 74, 361
BspLI GGNNCC 2 cut(s) 200, 751
BspPI GGATC 3 cut(s) 384, 744, 757
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 539
BsrI ACTGG 1 cut(s) 777
BssECI CCNNGG 1 cut(s) 715
BssMI GATC 3 cut(s) 362, 376, 749
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 3 cut(s) 25, 239, 928
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 42, 588
BstDEI CTNAG 3 cut(s) 693, 822, 916
BstFNI CGCG 3 cut(s) 32, 74, 361
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 365, 379, 752
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 3 cut(s) 362, 376, 749
BstMWI GCNNNNNNNGC 4 cut(s) 26, 80, 686, 711
BstNI CCWGG 1 cut(s) 717
BstSCI CCNGG 2 cut(s) 164, 715
BstSFI CTRYAG 2 cut(s) 399, 927
BstUI CGCG 3 cut(s) 32, 74, 361
BstV1I GCAGC 2 cut(s) 575, 664
BstV2I GAAGAC 4 cut(s) 144, 162, 216, 244
BstX2I RGATCY 2 cut(s) 376, 749
BstYI RGATCY 2 cut(s) 376, 749
BsuRI GGCC 3 cut(s) 20, 714, 892
Cac8I GCNNGC 2 cut(s) 42, 588
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 4 cut(s) 96, 162, 713, 890
CpoI CGGWCCG 1 cut(s) 96
CseI GACGC 3 cut(s) 63, 313, 592
Csp6I GTAC 1 cut(s) 199
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CspI CGGWCCG 1 cut(s) 96
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 693, 822, 916
DpnI GATC 3 cut(s) 364, 378, 751
DpnII GATC 3 cut(s) 362, 376, 749
DriI GACNNNNNGTC 2 cut(s) 190, 248
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 2 cut(s) 190, 248
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 3 cut(s) 67, 117, 138
Eco47I GGWCC 2 cut(s) 96, 162
Eco57I CTGAAG 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 715
FaiI YATR 9 cut(s) 263, 407, 554, 608, 654, 656, 867, 895, 897
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 864, 896
FauI CCCGC 1 cut(s) 855
FauNDI CATATG 1 cut(s) 895
FblI GTMKAC 2 cut(s) 410, 416
Fnu4HI GCNGC 5 cut(s) 30, 81, 298, 564, 678
Fsp4HI GCNGC 5 cut(s) 30, 81, 298, 564, 678
FspBI CTAG 2 cut(s) 702, 940
GlaI GCGC 1 cut(s) 360
GluI GCNGC 5 cut(s) 30, 81, 298, 564, 678
HaeIII GGCC 3 cut(s) 20, 714, 892
HapII CCGG 4 cut(s) 17, 99, 120, 165
HgaI GACGC 3 cut(s) 63, 313, 592
HhaI GCGC 1 cut(s) 361
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 6 cut(s) 128, 185, 243, 742, 793, 902
HpaII CCGG 4 cut(s) 17, 99, 120, 165
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 7 cut(s) 242, 411, 417, 438, 598, 643, 792
Hpy188I TCNGA 4 cut(s) 61, 184, 235, 514
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 7 cut(s) 242, 411, 417, 438, 598, 643, 792
Hpy99I CGWCG 7 cut(s) 29, 214, 217, 220, 223, 235, 329
HpyAV CCTTC 3 cut(s) 820, 866, 881
HpyCH4III ACNGT 3 cut(s) 25, 239, 928
HpyCH4IV ACGT 2 cut(s) 249, 413
HpyCH4V TGCA 2 cut(s) 480, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 80, 686, 711
HpyF3I CTNAG 3 cut(s) 693, 822, 916
HpySE526I ACGT 2 cut(s) 249, 413
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 362, 376, 749
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 686
Lsp1109I GCAGC 2 cut(s) 575, 664
LweI GCATC 4 cut(s) 455, 489, 514, 714
MaeI CTAG 2 cut(s) 702, 940
MaeII ACGT 2 cut(s) 249, 413
MaeIII GTNAC 6 cut(s) 499, 580, 661, 719, 876, 922
MalI GATC 3 cut(s) 364, 378, 751
MboI GATC 3 cut(s) 362, 376, 749
MflI RGATCY 2 cut(s) 376, 749
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 3 cut(s) 67, 569, 666
MlyI GAGTC 4 cut(s) 179, 237, 751, 787
MmeI TCCRAC 2 cut(s) 84, 213
MnlI CCTC 6 cut(s) 127, 238, 769, 775, 807, 849
MseI TTAA 2 cut(s) 381, 837
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 4 cut(s) 17, 99, 120, 165
MspR9I CCNGG 2 cut(s) 166, 717
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 3 cut(s) 32, 74, 361
MwoI GCNNNNNNNGC 4 cut(s) 26, 80, 686, 711
NciI CCSGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 895
NdeII GATC 3 cut(s) 362, 376, 749
NlaIV GGNNCC 2 cut(s) 200, 751
NmuCI GTSAC 3 cut(s) 580, 719, 876
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 4 cut(s) 155, 212, 218, 227
PfeI GAWTC 2 cut(s) 128, 902
PkrI GCNGC 5 cut(s) 31, 82, 299, 565, 679
PleI GAGTC 4 cut(s) 179, 237, 750, 787
PpsI GAGTC 4 cut(s) 179, 237, 750, 787
PsiI TTATAA 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 715
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 2 cut(s) 200, 751
PspPI GGNCC 4 cut(s) 96, 162, 713, 890
PsuI RGATCY 2 cut(s) 376, 749
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 2 cut(s) 381, 837
SatI GCNGC 5 cut(s) 30, 81, 298, 564, 678
Sau3AI GATC 3 cut(s) 362, 376, 749
Sau96I GGNCC 4 cut(s) 96, 162, 713, 890
SchI GAGTC 4 cut(s) 179, 237, 751, 787
ScrFI CCNGG 2 cut(s) 166, 717
SduI GDGCHC 1 cut(s) 691
SetI ASST 8 cut(s) 252, 336, 342, 416, 568, 694, 721, 786
SfaNI GCATC 4 cut(s) 455, 489, 514, 714
SfcI CTRYAG 2 cut(s) 399, 927
SinI GGWCC 2 cut(s) 96, 162
SmlI CTYRAG 2 cut(s) 484, 681
SmoI CTYRAG 2 cut(s) 484, 681
Sse9I AATT 3 cut(s) 67, 569, 666
SspMI CTAG 2 cut(s) 702, 940
StyD4I CCNGG 2 cut(s) 164, 715
TaaI ACNGT 3 cut(s) 25, 239, 928
TaiI ACGT 2 cut(s) 252, 416
TaqI TCGA 3 cut(s) 385, 505, 761
TasI AATT 3 cut(s) 67, 569, 666
TauI GCSGC 3 cut(s) 32, 83, 300
TfiI GAWTC 2 cut(s) 128, 902
Tru1I TTAA 2 cut(s) 381, 837
Tru9I TTAA 2 cut(s) 381, 837
TseFI GTSAC 3 cut(s) 580, 719, 876
TseI GCWGC 2 cut(s) 563, 677
Tsp45I GTSAC 3 cut(s) 580, 719, 876
TspDTI ATGAA 7 cut(s) 126, 132, 144, 168, 483, 591, 885
VpaK11BI GGWCC 2 cut(s) 96, 162
XapI RAATTY 1 cut(s) 569
XmiI GTMKAC 2 cut(s) 410, 416
XspI CTAG 2 cut(s) 702, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.