MD12G1194800.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001828
Physical Location & Seq
Forward (+)
27610868 .. 27613770
2903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1194800.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGGAAGTGGGAAGGAGAATATCGGCAAGTCCTCGGCCGTGCTCCGTCCGGCGAGTGGTGGCGAAGAAGAGGGGTAGCTCCGATGGGTTCGTAAACAGCGTGAAGAAGCTTCAGAGGCGTGAGATTTCTTCGAAGCGTGATAGAGCTTTTGGGATGAGCAATTCCCAGGAGAGGTTCCGCAACATGCACCTCGTGGAGGAATATGATACTCATGACCCCAAGGGGCATTCTTCGGCCATATTGCCTTTTCTGATGAAAAGGACAAAGGTCATTGAGATAGTTGCCGCACGTGACATCGTCTTTGCTCTTGCACACTCTGGTGTTTGCGCAGCTTTTAGTAGAGAGAAAAACGAGAGGATTTGTTTCATGAATGTTAGTCCTGATGAAGTCATTCGGAGCTTGTTCTATAATAAGAACAACGATTCGCTCATCACAGTTTCGGTTTATGCATCAGACAACTTCAGCTCTTTGAAATGCAGATCCACAAGGATTGAATATATAAGGAGGGGACAGCCAGAAGCTGGCTTTGCTCTTTTCGAGTCTGAGTCTTTGAAGTGGCCTGGGTTTGTAGAGTTTGATGATGTTAATGGCAAGGTGCTGACATACTCAGCACAGGATAGTATATACAAGGTGTTTGACCTTAAAAACTATACCATGCTGTACTCTATATCAGACAAACATGTACAAGAAATCAAAATCAGTCCAGGCATCATGCTGTTGATTTATAACAGAGCTACTAGCCATGTTCCTCTGAAGATTCTATCAATAGAAGATGGCACAGTTCTCAAAGACTTCAATCATCTACTTCATAGAAATAAGAAGGTGGACTTCATAGAACAGTTCAATGAAAAGCTTCTCGTCAAGCAAGAAAATGAGAACCTTCAGATTCTGGATGTTCGAAATGCTGAGATGATGGAAGTTAACAGAACAGACTTCATAACTCCATCAGCATTTATTTTTCTGTATGAGAACCAGTTATTCCTGACGTTCAGAAATCGACTGGTGTCTGTGTGGAACTTTCGTGGAGAACTTGTAACTGCATTTGAGGATCACTTGTTATGGCATCCTGACTGCAATACCAATAACATTTATATAACAAGCGATCAGGATCTTATCATATCTTACTGCAAGGCTGATTCTGATGATCATTGGTCAGAAGCAAATGGTATCTCTTTCCACTTCCTTTTCAGTTTTTTGTACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

46.49

Weight (kDa)

7.67

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 48 - 389 9.3e-195 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
Acc16I TGCGCA 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 521
AciI CCGC 2 cut(s) 178, 285
AclWI GGATC 3 cut(s) 474, 1056, 1116
AcoI YGGCCR 2 cut(s) 35, 234
AcuI CTGAAG 4 cut(s) 95, 445, 773, 866
AcvI CACGTG 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 3 cut(s) 662, 684, 1199
AfiI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 5 cut(s) 472, 494, 553, 796, 844
AjnI CCWGG 3 cut(s) 165, 559, 703
AleI CACNNNNGTG 1 cut(s) 318
AluBI AGCT 9 cut(s) 78, 109, 146, 332, 399, 465, 521, 734, 853
AluI AGCT 9 cut(s) 78, 109, 146, 332, 399, 465, 521, 734, 853
Alw21I GWGCWC 1 cut(s) 44
AlwI GGATC 3 cut(s) 474, 1056, 1116
AlwNI CAGNNNCTG 2 cut(s) 521, 889
AoxI GGCC 3 cut(s) 35, 234, 557
ApeKI GCWGC 1 cut(s) 329
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 2 cut(s) 390, 852
AspLEI GCGC 1 cut(s) 329
AsuII TTCGAA 2 cut(s) 131, 898
BauI CACGAG 1 cut(s) 191
BbrPI CACGTG 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 341
BccI CCATC 4 cut(s) 77, 767, 907, 952
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 3 cut(s) 167, 561, 705
BclI TGATCA 1 cut(s) 1144
BfaI CTAG 2 cut(s) 738, 1204
BisI GCNGC 2 cut(s) 285, 330
BlsI GCNGC 2 cut(s) 286, 331
Bme1390I CCNGG 3 cut(s) 167, 561, 705
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 3 cut(s) 167, 561, 705
BmsI GCATC 3 cut(s) 458, 717, 1072
BoxI GACNNNNGTC 2 cut(s) 266, 1003
Bpu14I TTCGAA 2 cut(s) 131, 898
BsaAI YACGTR 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 1107
BsaJI CCNNGG 4 cut(s) 32, 165, 219, 560
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
Bse1I ACTGG 2 cut(s) 973, 1005
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 3 cut(s) 167, 561, 705
BseDI CCNNGG 4 cut(s) 32, 165, 219, 560
BseGI GGATG 3 cut(s) 159, 898, 1063
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
BseMII CTCAG 3 cut(s) 534, 621, 897
BseNI ACTGG 2 cut(s) 973, 1005
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 37, 236, 559
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
BsmFI GGGAC 1 cut(s) 522
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 3 cut(s) 37, 236, 559
Bsp119I TTCGAA 2 cut(s) 131, 898
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1407I TGTACA 2 cut(s) 682, 1197
Bsp143I GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
BspACI CCGC 2 cut(s) 178, 285
BspANI GGCC 3 cut(s) 37, 236, 559
BspCNI CTCAG 3 cut(s) 535, 620, 898
BspHI TCATGA 2 cut(s) 211, 366
BspLI GGNNCC 1 cut(s) 176
BspPI GGATC 3 cut(s) 474, 1056, 1116
BspT104I TTCGAA 2 cut(s) 131, 898
BsrGI TGTACA 2 cut(s) 682, 1197
BsrI ACTGG 2 cut(s) 973, 1005
BssECI CCNNGG 4 cut(s) 32, 165, 219, 560
BssMI GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 3 cut(s) 167, 561, 705
Bst4CI ACNGT 3 cut(s) 436, 781, 840
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 2 cut(s) 682, 1197
BstBAI YACGTR 1 cut(s) 290
BstBI TTCGAA 2 cut(s) 131, 898
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 3 cut(s) 543, 607, 906
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 3 cut(s) 159, 898, 1063
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 5 cut(s) 482, 1051, 1105, 1111, 1147
BstMBI GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 115, 527
BstNI CCWGG 3 cut(s) 167, 561, 705
BstNSI RCATGY 2 cut(s) 187, 683
BstPAI GACNNNNGTC 2 cut(s) 266, 1003
BstSCI CCNGG 3 cut(s) 165, 559, 703
BstV1I GCAGC 1 cut(s) 341
BstX2I RGATCY 2 cut(s) 479, 1108
BstYI RGATCY 2 cut(s) 479, 1108
BstZI CGGCCG 1 cut(s) 35
BsuRI GGCC 3 cut(s) 37, 236, 559
BtsCI GGATG 3 cut(s) 159, 898, 1063
Cac8I GCNNGC 1 cut(s) 523
CaiI CAGNNNCTG 2 cut(s) 521, 889
CciI TCATGA 2 cut(s) 211, 366
CfoI GCGC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 661, 683, 1198
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 7 cut(s) 184, 212, 367, 655, 680, 712, 743
CviQI GTAC 3 cut(s) 661, 683, 1198
DdeI CTNAG 3 cut(s) 543, 607, 906
DpnI GATC 5 cut(s) 481, 1050, 1104, 1110, 1146
DpnII GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
DraIII CACNNNGTG 1 cut(s) 193
EaeI YGGCCR 2 cut(s) 35, 234
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 219
Eco52I CGGCCG 1 cut(s) 35
Eco57I CTGAAG 4 cut(s) 95, 445, 773, 866
Eco72I CACGTG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoRII CCWGG 3 cut(s) 165, 559, 703
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 7 cut(s) 187, 215, 370, 658, 683, 715, 746
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 522
FatI CATG 7 cut(s) 183, 211, 366, 654, 679, 711, 742
FbaI TGATCA 1 cut(s) 1144
Fnu4HI GCNGC 2 cut(s) 285, 330
FokI GGATG 3 cut(s) 166, 905, 1050
Fsp4HI GCNGC 2 cut(s) 285, 330
FspBI CTAG 2 cut(s) 738, 1204
FspI TGCGCA 1 cut(s) 328
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 285, 330
HaeIII GGCC 3 cut(s) 37, 236, 559
HapII CCGG 1 cut(s) 49
HhaI GCGC 1 cut(s) 329
Hin1II CATG 7 cut(s) 187, 215, 370, 658, 683, 715, 746
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 2 cut(s) 107, 851
HinfI GANTC 6 cut(s) 422, 539, 545, 757, 886, 1136
HpaI GTTAAC 1 cut(s) 922
HpaII CCGG 1 cut(s) 49
Hpy166II GTNNAC 3 cut(s) 94, 826, 922
Hpy188III TCNNGA 7 cut(s) 212, 367, 380, 890, 982, 1067, 1106
Hpy8I GTNNAC 3 cut(s) 94, 826, 922
HpyAV CCTTC 3 cut(s) 6, 814, 890
HpyCH4III ACNGT 3 cut(s) 436, 781, 840
HpyCH4IV ACGT 2 cut(s) 289, 986
HpyCH4V TGCA 7 cut(s) 187, 311, 449, 477, 1040, 1074, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 527
HpyF3I CTNAG 3 cut(s) 543, 607, 906
HpySE526I ACGT 2 cut(s) 289, 986
Hsp92II CATG 7 cut(s) 187, 215, 370, 658, 683, 715, 746
HspAI GCGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 1144
KspAI GTTAAC 1 cut(s) 922
Kzo9I GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
LmnI GCTCC 3 cut(s) 47, 83, 396
Lsp1109I GCAGC 1 cut(s) 341
LweI GCATC 3 cut(s) 458, 717, 1072
MaeI CTAG 2 cut(s) 738, 1204
MaeII ACGT 2 cut(s) 289, 986
MaeIII GTNAC 2 cut(s) 290, 1033
MalI GATC 5 cut(s) 481, 1050, 1104, 1110, 1146
MboI GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
MboII GAAGA 7 cut(s) 76, 79, 115, 120, 222, 766, 782
MflI RGATCY 2 cut(s) 479, 1108
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 160
MlyI GAGTC 2 cut(s) 548, 554
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 2 cut(s) 390, 852
MseI TTAA 3 cut(s) 585, 642, 921
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 3 cut(s) 167, 561, 705
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 3 cut(s) 167, 561, 705
MwoI GCNNNNNNNGC 2 cut(s) 115, 527
NdeII GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
NlaIII CATG 7 cut(s) 187, 215, 370, 658, 683, 715, 746
NlaIV GGNNCC 1 cut(s) 176
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 187, 683
NspV TTCGAA 2 cut(s) 131, 898
OliI CACNNNNGTG 1 cut(s) 318
PagI TCATGA 2 cut(s) 211, 366
PciI ACATGT 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 2 cut(s) 390, 852
PfeI GAWTC 4 cut(s) 422, 757, 886, 1136
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 521
PkrI GCNGC 2 cut(s) 286, 331
PleI GAGTC 2 cut(s) 547, 553
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
PpsI GAGTC 2 cut(s) 547, 553
Ppu21I YACGTR 1 cut(s) 290
PscI ACATGT 1 cut(s) 679
PshAI GACNNNNGTC 2 cut(s) 266, 1003
PsiI TTATAA 1 cut(s) 726
Psp6I CCWGG 3 cut(s) 165, 559, 703
PspCI CACGTG 1 cut(s) 290
PspGI CCWGG 3 cut(s) 165, 559, 703
PspN4I GGNNCC 1 cut(s) 176
PstNI CAGNNNCTG 2 cut(s) 521, 889
PsuI RGATCY 2 cut(s) 479, 1108
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 3 cut(s) 662, 684, 1199
RsaNI GTAC 3 cut(s) 661, 683, 1198
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 3 cut(s) 585, 642, 921
SatI GCNGC 2 cut(s) 285, 330
Sau3AI GATC 5 cut(s) 479, 1048, 1102, 1108, 1144
SchI GAGTC 2 cut(s) 548, 554
ScrFI CCNGG 3 cut(s) 167, 561, 705
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 3 cut(s) 458, 717, 1072
SfuI TTCGAA 2 cut(s) 131, 898
SmiMI CAYNNNNRTG 1 cut(s) 318
Sse9I AATT 1 cut(s) 160
SsiI CCGC 2 cut(s) 178, 285
SspMI CTAG 2 cut(s) 738, 1204
StyD4I CCNGG 3 cut(s) 165, 559, 703
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 436, 781, 840
TaiI ACGT 2 cut(s) 292, 989
TaqI TCGA 4 cut(s) 131, 537, 898, 997
TasI AATT 1 cut(s) 160
TatI WGTACW 3 cut(s) 660, 682, 1197
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 4 cut(s) 422, 757, 886, 1136
Tru1I TTAA 3 cut(s) 585, 642, 921
Tru9I TTAA 3 cut(s) 585, 642, 921
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 8 cut(s) 269, 355, 383, 399, 797, 820, 861, 925
TspGWI ACGGA 1 cut(s) 34
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 521
XagI CCTNNNNNAGG 1 cut(s) 194
XceI RCATGY 2 cut(s) 187, 683
XmnI GAANNNNTTC 2 cut(s) 390, 852
XspI CTAG 2 cut(s) 738, 1204
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.