pycom04g15870
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001828
Physical Location & Seq
Forward (+)
18633003 .. 18636444
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15870.1

Sequence Viewer

Length: 1254 bp
ATGGAAGTGGGAAGGAGGATATCGGCGAGTCCTCGGCCGTGCTCGGGGCGGCGAGTGGTGGCGAAGAAGAGGAGGGGTGGTTCCGATGGGTTCGTTAACAGCGTGAAGAAGCTTCAGAGGCGTGAGATTTCTTCGAAGCGTGATCGGGCTTTTGGGATGAGCAATTCCCAGGAGAGGTTCCGCAACATGCACCTCGTGGAGCAATATGATACTCATGATCCCAAGGGGCATTCTTCGGCCATATTGCCTTTTCTGATGAAAAGGACAAAGGTCATTGAGATAGTTTCTGCACGTGACATCGTCTTTGCTCTTGCACACTCTGGTGTTTGCGCAGCTTTTAGTAGAGAGAAAAACGAGAGGATTTGTTTCATGAATGTTAGTCCTGATGAAGTTATTCGAAGCTTGTTCTATAATAAGAACAACGATTCACTCATCACAGTTTCGGTGTATGCTTCAGACAACTTCAGCTCTTTGAAATGCAGATCCACAAGGATTGAATATATAAGGAGGGGACAGCCAGAAGCTGGTTTTTCTCTTTTTGAGTCTGAGTCTTTGAAGTGGCCTGGGTTTGTAGAGTTTGATGATGTTAATGGCAAGGTGCTGACATACTCAGCACAAGATAGTATATACAAGGTGTTTGATCTGAAAAACTATACCATGCTGTACTCTATATCAGACAAACATGTCCAAGAAATCAAAATCAGTCCAGGCATCATGCTGTTGATTTATAACAGAGCTAGTAGCCATGTTCCTCTGAAAATTCTGTCAATAGAAGATGGCACAGTTCTCAAAGACTTCAATCATCTACTTCATAGAAATAAGAAGGTGGACTTCATAGAACAGTTCAACGAAAAGCTTCTTGTCAAGCAAGAAAATGAGAATCTTCAGATTCTGGATGTTCGAAATGCTGAGCTGATGGAAGTTAGCAGAACAGACTTCATAACTCCATCAGCATTTATCTTTCTGTATGAGAACCAGCTATTCCTGACATTCAGAAATCGATTGGTGTCTGTCTGGAACTTTCGTGGAGAACTTGTCACTGCGTTTGAGGATCGCTTGTTATGGCATCCTGACTGCAATACCAATAACATTTATATAACAAGCGATCAGGATCTTATCATATCCTACTGCAAGGCCGATTCTGATCGATCATTGGTCAGAAGGAAATGCTGGATCTATTCATGTCAGCAATATCTTAACCGGAAAATGCCTGGCCAAAATAAATCTGAGCAATGGTGGTCCCAAGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

48.53

Weight (kDa)

9.29

Isoelectric Point (pI)

53.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 49 - 384 1.4e-192 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AasI GACNNNNNNGTC 1 cut(s) 683
Acc16I TGCGCA 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 524
AciI CCGC 2 cut(s) 49, 181
AclWI GGATC 5 cut(s) 212, 477, 1059, 1119, 1181
AcoI YGGCCR 3 cut(s) 35, 237, 1213
AcsI RAATTY 1 cut(s) 759
AcuI CTGAAG 4 cut(s) 98, 438, 448, 869
AcvI CACGTG 1 cut(s) 293
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 1 cut(s) 665
AfiI CCNNNNNNNGG 3 cut(s) 44, 174, 524
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 5 cut(s) 475, 497, 556, 799, 847
AjnI CCWGG 4 cut(s) 168, 562, 706, 1210
AleI CACNNNNGTG 1 cut(s) 321
AloI GAACNNNNNNTCC 2 cut(s) 64, 96
AluBI AGCT 9 cut(s) 112, 335, 402, 468, 524, 737, 856, 913, 979
AluI AGCT 9 cut(s) 112, 335, 402, 468, 524, 737, 856, 913, 979
Alw21I GWGCWC 1 cut(s) 44
AlwI GGATC 5 cut(s) 212, 477, 1059, 1119, 1181
AlwNI CAGNNNCTG 2 cut(s) 524, 892
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 5 cut(s) 35, 237, 560, 1134, 1213
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 759
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 2 cut(s) 393, 855
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 1239
AsuII TTCGAA 3 cut(s) 134, 397, 901
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 1215
BauI CACGAG 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 344
BccI CCATC 4 cut(s) 80, 770, 910, 955
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 4 cut(s) 170, 564, 708, 1212
BfaI CTAG 1 cut(s) 738
BisI GCNGC 2 cut(s) 50, 333
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 2 cut(s) 51, 334
Bme1390I CCNGG 4 cut(s) 170, 564, 708, 1212
Bme18I GGWCC 1 cut(s) 1239
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 1239
BmiI GGNNCC 3 cut(s) 82, 179, 1241
BmrFI CCNGG 4 cut(s) 170, 564, 708, 1212
BmsI GCATC 2 cut(s) 720, 1075
BoxI GACNNNNGTC 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 3 cut(s) 134, 397, 901
Bsa29I ATCGAT 2 cut(s) 1000, 1147
BsaAI YACGTR 1 cut(s) 293
BsaBI GATNNNNATC 2 cut(s) 1110, 1143
BsaJI CCNNGG 5 cut(s) 32, 168, 222, 563, 1243
BsaWI WCCGGW 1 cut(s) 1200
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 1020, 1050
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 174, 524
Bse3DI GCAATG 1 cut(s) 1238
Bse8I GATNNNNATC 2 cut(s) 1110, 1143
BseBI CCWGG 4 cut(s) 170, 564, 708, 1212
BseCI ATCGAT 2 cut(s) 1000, 1147
BseDI CCNNGG 5 cut(s) 32, 168, 222, 563, 1243
BseGI GGATG 3 cut(s) 162, 901, 1066
BseJI GATNNNNATC 2 cut(s) 1110, 1143
BseLI CCNNNNNNNGG 3 cut(s) 44, 174, 524
BseMI GCAATG 1 cut(s) 1238
BseMII CTCAG 4 cut(s) 537, 624, 900, 1218
BseRI GAGGAG 1 cut(s) 85
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 5 cut(s) 37, 239, 562, 1136, 1215
BshVI ATCGAT 2 cut(s) 1000, 1147
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 1 cut(s) 1201
BslFI GGGAC 2 cut(s) 525, 1225
BslI CCNNNNNNNGG 3 cut(s) 44, 174, 524
BsmFI GGGAC 2 cut(s) 525, 1225
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 5 cut(s) 37, 239, 562, 1136, 1215
BsoBI CYCGRG 1 cut(s) 43
Bsp119I TTCGAA 3 cut(s) 134, 397, 901
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1720I GCTNAGC 1 cut(s) 909
BspACI CCGC 2 cut(s) 49, 181
BspANI GGCC 5 cut(s) 37, 239, 562, 1136, 1215
BspCNI CTCAG 4 cut(s) 538, 623, 901, 1219
BspDI ATCGAT 2 cut(s) 1000, 1147
BspHI TCATGA 2 cut(s) 214, 369
BspLI GGNNCC 3 cut(s) 82, 179, 1241
BspPI GGATC 5 cut(s) 212, 477, 1059, 1119, 1181
BspT104I TTCGAA 3 cut(s) 134, 397, 901
BsrDI GCAATG 1 cut(s) 1238
BssECI CCNNGG 5 cut(s) 32, 168, 222, 563, 1243
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 2 cut(s) 222, 1243
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 4 cut(s) 170, 564, 708, 1212
Bst4CI ACNGT 3 cut(s) 439, 784, 843
Bst6I CTCTTC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 3 cut(s) 134, 397, 901
BstDEI CTNAG 4 cut(s) 546, 610, 909, 1227
BstF5I GGATG 3 cut(s) 162, 901, 1066
BstHHI GCGC 1 cut(s) 332
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 4 cut(s) 170, 564, 708, 1212
BstNSI RCATGY 2 cut(s) 190, 686
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 4 cut(s) 168, 562, 706, 1210
BstV1I GCAGC 1 cut(s) 344
BstX2I RGATCY 3 cut(s) 482, 1111, 1173
BstYI RGATCY 3 cut(s) 482, 1111, 1173
BstZI CGGCCG 1 cut(s) 35
Bsu15I ATCGAT 2 cut(s) 1000, 1147
BsuRI GGCC 5 cut(s) 37, 239, 562, 1136, 1215
BsuTUI ATCGAT 2 cut(s) 1000, 1147
BtsCI GGATG 3 cut(s) 162, 901, 1066
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 1 cut(s) 1038
CaiI CAGNNNCTG 2 cut(s) 524, 892
CciI TCATGA 2 cut(s) 214, 369
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 1239
ClaI ATCGAT 2 cut(s) 1000, 1147
Csp6I GTAC 1 cut(s) 664
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 8 cut(s) 187, 215, 370, 658, 683, 715, 746, 1182
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 4 cut(s) 546, 610, 909, 1227
DraIII CACNNNGTG 1 cut(s) 196
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 3 cut(s) 35, 237, 1213
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 222, 1243
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 1239
Eco52I CGGCCG 1 cut(s) 35
Eco57I CTGAAG 4 cut(s) 98, 438, 448, 869
Eco72I CACGTG 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 4 cut(s) 168, 562, 706, 1210
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 222, 1243
ErhI CCWWGG 2 cut(s) 222, 1243
FaeI CATG 8 cut(s) 190, 218, 373, 661, 686, 718, 749, 1185
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 2 cut(s) 525, 1225
FatI CATG 8 cut(s) 186, 214, 369, 657, 682, 714, 745, 1181
Fnu4HI GCNGC 2 cut(s) 50, 333
FokI GGATG 3 cut(s) 169, 908, 1053
Fsp4HI GCNGC 2 cut(s) 50, 333
FspBI CTAG 1 cut(s) 738
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 50, 333
HaeIII GGCC 5 cut(s) 37, 239, 562, 1136, 1215
HapII CCGG 1 cut(s) 1201
HhaI GCGC 1 cut(s) 332
Hin1II CATG 8 cut(s) 190, 218, 373, 661, 686, 718, 749, 1185
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 3 cut(s) 110, 400, 854
HinfI GANTC 7 cut(s) 28, 425, 542, 548, 880, 889, 1139
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 1 cut(s) 1201
Hpy166II GTNNAC 2 cut(s) 97, 829
Hpy188III TCNNGA 8 cut(s) 215, 370, 383, 893, 985, 1015, 1070, 1109
Hpy8I GTNNAC 2 cut(s) 97, 829
HpyAV CCTTC 3 cut(s) 6, 817, 1155
HpyCH4III ACNGT 3 cut(s) 439, 784, 843
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 6 cut(s) 190, 290, 314, 480, 1077, 1131
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 4 cut(s) 546, 610, 909, 1227
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 8 cut(s) 190, 218, 373, 661, 686, 718, 749, 1185
HspAI GCGC 1 cut(s) 330
KspAI GTTAAC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 2 cut(s) 720, 1075
MaeI CTAG 1 cut(s) 738
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 293, 1036
MboII GAAGA 7 cut(s) 76, 79, 118, 123, 225, 785, 875
MflI RGATCY 3 cut(s) 482, 1111, 1173
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 1215
MluCI AATT 2 cut(s) 163, 759
MluNI TGGCCA 1 cut(s) 1215
MlyI GAGTC 3 cut(s) 37, 551, 557
Mox20I TGGCCA 1 cut(s) 1215
MroXI GAANNNNTTC 2 cut(s) 393, 855
MscI TGGCCA 1 cut(s) 1215
MseI TTAA 3 cut(s) 96, 588, 1197
MslI CAYNNNNRTG 1 cut(s) 321
Msp20I TGGCCA 1 cut(s) 1215
MspI CCGG 1 cut(s) 1201
MspR9I CCNGG 4 cut(s) 170, 564, 708, 1212
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 4 cut(s) 170, 564, 708, 1212
MwoI GCNNNNNNNGC 1 cut(s) 118
NlaIII CATG 8 cut(s) 190, 218, 373, 661, 686, 718, 749, 1185
NlaIV GGNNCC 3 cut(s) 82, 179, 1241
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 2 cut(s) 293, 1036
NsbI TGCGCA 1 cut(s) 331
NspI RCATGY 2 cut(s) 190, 686
NspV TTCGAA 3 cut(s) 134, 397, 901
OliI CACNNNNGTG 1 cut(s) 321
PagI TCATGA 2 cut(s) 214, 369
PciI ACATGT 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 2 cut(s) 393, 855
PfeI GAWTC 4 cut(s) 425, 880, 889, 1139
PflFI GACNNNGTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 2 cut(s) 51, 334
PleI GAGTC 3 cut(s) 36, 550, 556
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 3 cut(s) 36, 550, 556
Ppu21I YACGTR 1 cut(s) 293
PscI ACATGT 1 cut(s) 682
PshAI GACNNNNGTC 1 cut(s) 269
PsiI TTATAA 1 cut(s) 729
Psp6I CCWGG 4 cut(s) 168, 562, 706, 1210
PspCI CACGTG 1 cut(s) 293
PspGI CCWGG 4 cut(s) 168, 562, 706, 1210
PspN4I GGNNCC 3 cut(s) 82, 179, 1241
PspPI GGNCC 1 cut(s) 1239
PstNI CAGNNNCTG 2 cut(s) 524, 892
PsuI RGATCY 3 cut(s) 482, 1111, 1173
PsyI GACNNNGTC 1 cut(s) 299
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 3 cut(s) 96, 588, 1197
SatI GCNGC 2 cut(s) 50, 333
Sau96I GGNCC 1 cut(s) 1239
SchI GAGTC 3 cut(s) 37, 551, 557
ScrFI CCNGG 4 cut(s) 170, 564, 708, 1212
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 2 cut(s) 720, 1075
SfuI TTCGAA 3 cut(s) 134, 397, 901
SinI GGWCC 1 cut(s) 1239
SmiMI CAYNNNNRTG 1 cut(s) 321
Sse9I AATT 2 cut(s) 163, 759
SsiI CCGC 2 cut(s) 49, 181
SspMI CTAG 1 cut(s) 738
StyD4I CCNGG 4 cut(s) 168, 562, 706, 1210
StyI CCWWGG 2 cut(s) 222, 1243
TaaI ACNGT 3 cut(s) 439, 784, 843
TaiI ACGT 1 cut(s) 295
TaqI TCGA 6 cut(s) 134, 397, 901, 1000, 1147, 1249
TasI AATT 2 cut(s) 163, 759
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 4 cut(s) 425, 880, 889, 1139
Tru1I TTAA 3 cut(s) 96, 588, 1197
Tru9I TTAA 3 cut(s) 96, 588, 1197
TscAI CASTG 1 cut(s) 1045
TseFI GTSAC 2 cut(s) 293, 1036
TseI GCWGC 1 cut(s) 332
Tsp45I GTSAC 2 cut(s) 293, 1036
TspDTI ATGAA 8 cut(s) 272, 358, 386, 402, 800, 823, 928, 1170
TspRI CASTG 1 cut(s) 1045
Tth111I GACNNNGTC 1 cut(s) 299
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 1239
XapI RAATTY 1 cut(s) 759
XceI RCATGY 2 cut(s) 190, 686
XmnI GAANNNNTTC 2 cut(s) 393, 855
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.