MD07G1238900.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001828
Physical Location & Seq
Forward (+)
31060619 .. 31063994
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1238900.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGGAAGGGGGAAGGAGAATCTCGGCGAGTCCTCGGCCTTGCAACGGTAGGAGGGTGGTGGCGAAGAAACGACCTCGTATTGGTGGGGTTGATGGGTTCGTCAACAGCGTTAAGAAGCTCCAGAGGCGCGAAATCAGCTCCAAGCGCGACCGTGCCTTCACTATGAGCGATGCCCAAGAGAGGTTTCGCAATATCCGCCTCCAGGAGGAATATGATACGCATGATCCTAAGGGTCATTGTGCCATGGTTCTGCCTTTCCTGCGAAAGAGGTCCAAAATTATTGAAATTGTAGCAGCACGTGACATCGTGTTTGCCCTCGCACAATCCGGTGTTTGTGCAGCATTTAGCCGAGAAACCAACCAGAGAATATGCTTTCTGAATGTCAGTCCTGATGAAGTGATAAGAAGCTTGTTTTATAATAAAAACAATGACTCACTTATCACCGTTTCGGTTTATGCCTCAGACAACTTCAGTTCTTTGAAATGCAGAAGCACAAGGATTGAATACATAAGAAGGGGTAAACCAGATGCTGGATTTGCTCTTTTTGAATCCGAGTCACTGAAATGGCCTGGTTTTGTAGAGTTTGATGATGTAAATGGGAAGGTTCTTACTTATTCTGCACAGGATAGTATATACAAGGTGTTTGATCTGAAAAACTACACAATGTTGTACTCCATATCGGATAAGAATGTCCAAGAGATTAAGATCAGCCCTGGGATCATGCTGTTGATTTTTACTAAAGCTAGTAGCCATGTTCCTCTTAAGATTCTGTCTATAGAGGATGGTACTGTCCTTAAATCCTTTAACCATCTCCTTCATCGGAATAAGAAGGTGGATTTCATCGAACAGTTCAACGAAAAGCTTCTTGTAAAGCAAGAAAATGAGAACCTTCAAATTCTTGATGTTCGCAATTTTGAGATGACAGAAGTAAGCAGAACTCAATTCATGACACCATCAGCCTTCATATTTCTGTATGAGAATCAGTTATTCCTGACGTTTCGGAATCGAACCGTGGCTGTTTGGAACTTTAGAGGAGAACTTGTGACTTCGTTTGAGGATCATCTTTTGTGGCATCCCGACTGCAATACCAATAACATATACATTACGAGTGACCAGGATCTTATCATTTCTTACTGCAAGGCCGATTCTGACGATCCATTATCTGAAGGAAATGCTGGATCCATAAATATCAGCAATATCTTGACCGGAAAATGCCTTGCTAAGATAAGAGCAACCAACAACGCTGAAAAAGAATGCACTTGTAGTGGTGAGTCTTGTGGTAGCAGTTGCAATTCGAAGAAGCGGGCCCTTTCATCCCGAATTAGGAGTACAGTTGCAGAAGCCTTGGAAGACATTACTGCTCTCTTCTACGACGAAGAGCGCAACGAGATTTACACGGGCAATAGGCTCGGTCTAGTTCACGTATGGTCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

479

Amino Acids

54.48

Weight (kDa)

8.7

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 51 - 476 1.2e-226 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 530
AccII CGCG 2 cut(s) 129, 147
AciI CCGC 2 cut(s) 196, 1303
AclWI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
AcsI RAATTY 1 cut(s) 894
AcuI CTGAAG 2 cut(s) 454, 1185
AcvI CACGTG 1 cut(s) 299
AfaI GTAC 3 cut(s) 671, 787, 1330
AfiI CCNNNNNNNGG 7 cut(s) 44, 80, 180, 202, 205, 530, 1323
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 6 cut(s) 284, 481, 503, 548, 853, 893
AjnI CCWGG 4 cut(s) 201, 568, 712, 1113
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 5 cut(s) 118, 138, 408, 743, 862
AluI AGCT 5 cut(s) 118, 138, 408, 743, 862
AlwI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 4 cut(s) 35, 566, 1140, 1305
ApaI GGGCCC 1 cut(s) 1309
ApeKI GCWGC 2 cut(s) 293, 338
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 129, 147, 1383
AspS9I GGNCC 3 cut(s) 270, 1305, 1306
AsuHPI GGTGA 2 cut(s) 433, 1280
AsuII TTCGAA 1 cut(s) 1295
AvaII GGWCC 1 cut(s) 270
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 1309
BamHI GGATCC 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 1309
BbrPI CACGTG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 1356
BbvI GCAGC 2 cut(s) 305, 350
BccI CCATC 4 cut(s) 86, 776, 816, 961
BcgI CGANNNNNNTGC 2 cut(s) 1390, 1424
BciT130I CCWGG 4 cut(s) 203, 570, 714, 1115
BfaI CTAG 2 cut(s) 744, 1415
BfmI CTRYAG 1 cut(s) 774
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 2 cut(s) 294, 339
BlsI GCNGC 2 cut(s) 295, 340
Bme1390I CCNGG 4 cut(s) 203, 570, 714, 1115
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 270, 1305, 1306
BmiI GGNNCC 2 cut(s) 1180, 1307
BmrFI CCNGG 4 cut(s) 203, 570, 714, 1115
BmsI GCATC 3 cut(s) 160, 517, 1081
BpiI GAAGAC 1 cut(s) 1356
BpmI CTGGAG 2 cut(s) 104, 185
Bpu14I TTCGAA 1 cut(s) 1295
BsaAI YACGTR 2 cut(s) 299, 1423
BsaBI GATNNNNATC 1 cut(s) 704
BsaJI CCNNGG 6 cut(s) 32, 243, 712, 713, 1011, 1344
BsaWI WCCGGW 2 cut(s) 326, 1205
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 7 cut(s) 44, 80, 180, 202, 205, 530, 1323
Bse21I CCTNAGG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 4 cut(s) 203, 570, 714, 1115
BseDI CCNNGG 6 cut(s) 32, 243, 712, 713, 1011, 1344
BseGI GGATG 3 cut(s) 787, 1072, 1313
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 7 cut(s) 44, 80, 180, 202, 205, 530, 1323
BseMII CTCAG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 1047
BseSI GKGCMC 1 cut(s) 1309
BseXI GCAGC 2 cut(s) 305, 350
BsgI GTGCAG 2 cut(s) 357, 603
Bsh1236I CGCG 2 cut(s) 129, 147
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 4 cut(s) 37, 568, 1142, 1307
BsiEI CGRYCG 1 cut(s) 151
BsiSI CCGG 2 cut(s) 327, 1206
BslI CCNNNNNNNGG 7 cut(s) 44, 80, 180, 202, 205, 530, 1323
BsmI GAATGC 1 cut(s) 1259
BsnI GGCC 4 cut(s) 37, 568, 1142, 1307
Bsp119I TTCGAA 1 cut(s) 1295
Bsp120I GGGCCC 1 cut(s) 1305
Bsp1286I GDGCHC 1 cut(s) 1309
Bsp143I GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 2 cut(s) 196, 1303
BspANI GGCC 4 cut(s) 37, 568, 1142, 1307
BspCNI CTCAG 1 cut(s) 473
BspFNI CGCG 2 cut(s) 129, 147
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 1180, 1307
BspPI GGATC 7 cut(s) 218, 725, 1065, 1125, 1148, 1173, 1186
BspQI GCTCTTC 1 cut(s) 1371
BspT104I TTCGAA 1 cut(s) 1295
BspTI CTTAAG 1 cut(s) 761
BssECI CCNNGG 6 cut(s) 32, 243, 712, 713, 1011, 1344
BssMI GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
BssT1I CCWWGG 2 cut(s) 243, 1344
Bst2UI CCWGG 4 cut(s) 203, 570, 714, 1115
Bst4CI ACNGT 7 cut(s) 47, 152, 445, 790, 849, 1012, 1333
Bst6I CTCTTC 2 cut(s) 1370, 1371
BstAFI CTTAAG 1 cut(s) 761
BstBAI YACGTR 2 cut(s) 299, 1423
BstBI TTCGAA 1 cut(s) 1295
BstC8I GCNNGC 1 cut(s) 1305
BstDEI CTNAG 3 cut(s) 228, 460, 1221
BstDSI CCRYGG 2 cut(s) 243, 1011
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 3 cut(s) 787, 1072, 1313
BstFNI CGCG 2 cut(s) 129, 147
BstHHI GCGC 3 cut(s) 129, 147, 1383
BstKTI GATC 8 cut(s) 226, 649, 708, 720, 1060, 1120, 1156, 1181
BstMBI GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
BstNI CCWGG 4 cut(s) 203, 570, 714, 1115
BstSCI CCNGG 4 cut(s) 201, 568, 712, 1113
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 1 cut(s) 1309
BstUI CGCG 2 cut(s) 129, 147
BstV1I GCAGC 2 cut(s) 305, 350
BstV2I GAAGAC 1 cut(s) 1356
BstX2I RGATCY 2 cut(s) 1117, 1178
BstYI RGATCY 2 cut(s) 1117, 1178
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 4 cut(s) 37, 568, 1142, 1307
BtgI CCRYGG 2 cut(s) 243, 1011
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 3 cut(s) 787, 1072, 1313
BtsIMutI CAGTG 1 cut(s) 557
Cac8I GCNNGC 1 cut(s) 1305
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 945
CfoI GCGC 3 cut(s) 129, 147, 1383
Cfr13I GGNCC 3 cut(s) 270, 1305, 1306
Csp6I GTAC 3 cut(s) 670, 786, 1329
CviAII CATG 5 cut(s) 221, 244, 721, 752, 946
CviQI GTAC 3 cut(s) 670, 786, 1329
DdeI CTNAG 3 cut(s) 228, 460, 1221
DpnI GATC 8 cut(s) 225, 648, 707, 719, 1059, 1119, 1155, 1180
DpnII GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
Eam1104I CTCTTC 2 cut(s) 1370, 1371
EarI CTCTTC 2 cut(s) 1370, 1371
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 243, 1344
Eco24I GRGCYC 1 cut(s) 1309
Eco47I GGWCC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 454, 1185
Eco72I CACGTG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 1306
EcoRII CCWGG 4 cut(s) 201, 568, 712, 1113
EcoT14I CCWWGG 2 cut(s) 243, 1344
EcoT38I GRGCYC 1 cut(s) 1309
ErhI CCWWGG 2 cut(s) 243, 1344
FaeI CATG 5 cut(s) 224, 247, 724, 755, 949
FatI CATG 5 cut(s) 220, 243, 720, 751, 945
FauI CCCGC 1 cut(s) 1296
Fnu4HI GCNGC 2 cut(s) 294, 339
FokI GGATG 3 cut(s) 794, 1059, 1300
FriOI GRGCYC 1 cut(s) 1309
Fsp4HI GCNGC 2 cut(s) 294, 339
FspBI CTAG 2 cut(s) 744, 1415
GlaI GCGC 3 cut(s) 128, 146, 1382
GluI GCNGC 2 cut(s) 294, 339
GsuI CTGGAG 2 cut(s) 104, 185
HaeIII GGCC 4 cut(s) 37, 568, 1142, 1307
HapII CCGG 2 cut(s) 327, 1206
HhaI GCGC 3 cut(s) 129, 147, 1383
Hin1II CATG 5 cut(s) 224, 247, 724, 755, 949
Hin6I GCGC 3 cut(s) 127, 145, 1381
HinP1I GCGC 3 cut(s) 127, 145, 1381
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 2 cut(s) 406, 860
HpaII CCGG 2 cut(s) 327, 1206
HphI GGTGA 2 cut(s) 433, 1280
Hpy166II GTNNAC 3 cut(s) 103, 521, 1420
Hpy188I TCNGA 9 cut(s) 378, 463, 553, 651, 682, 822, 1002, 1150, 1165
Hpy188III TCNNGA 8 cut(s) 121, 389, 899, 946, 991, 1076, 1201, 1317
Hpy8I GTNNAC 3 cut(s) 103, 521, 1420
Hpy99I CGWCG 1 cut(s) 1376
HpyAV CCTTC 9 cut(s) 6, 166, 507, 595, 823, 824, 899, 970, 1160
HpyCH4III ACNGT 7 cut(s) 47, 152, 445, 790, 849, 1012, 1333
HpyCH4IV ACGT 3 cut(s) 298, 995, 1422
HpyCH4V TGCA 9 cut(s) 42, 338, 486, 620, 1083, 1137, 1257, 1290, 1337
HpyF10VI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
HpyF3I CTNAG 3 cut(s) 228, 460, 1221
HpySE526I ACGT 3 cut(s) 298, 995, 1422
Hsp92II CATG 5 cut(s) 224, 247, 724, 755, 949
HspAI GCGC 3 cut(s) 127, 145, 1381
Kzo9I GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 2 cut(s) 123, 143
Lsp1109I GCAGC 2 cut(s) 305, 350
LweI GCATC 3 cut(s) 160, 517, 1081
MaeI CTAG 2 cut(s) 744, 1415
MaeII ACGT 3 cut(s) 298, 995, 1422
MaeIII GTNAC 4 cut(s) 299, 555, 1042, 1109
MalI GATC 8 cut(s) 225, 648, 707, 719, 1059, 1119, 1155, 1180
MboI GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
MboII GAAGA 5 cut(s) 76, 1309, 1357, 1361, 1388
MflI RGATCY 2 cut(s) 1117, 1178
MhlI GDGCHC 1 cut(s) 1309
MluCI AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
MlyI GAGTC 4 cut(s) 37, 425, 563, 1280
MroXI GAANNNNTTC 1 cut(s) 861
MseI TTAA 5 cut(s) 111, 702, 762, 795, 804
MslI CAYNNNNRTG 1 cut(s) 562
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 2 cut(s) 327, 1206
MspR9I CCNGG 4 cut(s) 203, 570, 714, 1115
Mva1269I GAATGC 1 cut(s) 1259
MvaI CCWGG 4 cut(s) 203, 570, 714, 1115
MvnI CGCG 2 cut(s) 129, 147
MwoI GCNNNNNNNGC 6 cut(s) 124, 135, 144, 195, 259, 536
NcoI CCATGG 1 cut(s) 243
NdeII GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
NlaIII CATG 5 cut(s) 224, 247, 724, 755, 949
NlaIV GGNNCC 2 cut(s) 1180, 1307
NmeAIII GCCGAG 2 cut(s) 13, 374
NmuCI GTSAC 4 cut(s) 299, 555, 1042, 1109
NspV TTCGAA 1 cut(s) 1295
PagI TCATGA 1 cut(s) 945
PasI CCCWGGG 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 1371
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 1259
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 6 cut(s) 18, 548, 766, 979, 1003, 1145
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 2 cut(s) 295, 340
PleI GAGTC 4 cut(s) 36, 425, 562, 1279
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
PpsI GAGTC 4 cut(s) 36, 425, 562, 1279
Ppu21I YACGTR 2 cut(s) 299, 1423
PsiI TTATAA 1 cut(s) 417
Psp6I CCWGG 4 cut(s) 201, 568, 712, 1113
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 4 cut(s) 201, 568, 712, 1113
PspN4I GGNNCC 2 cut(s) 1180, 1307
PspOMI GGGCCC 1 cut(s) 1305
PspPI GGNCC 3 cut(s) 270, 1305, 1306
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 2 cut(s) 1117, 1178
RsaI GTAC 3 cut(s) 671, 787, 1330
RsaNI GTAC 3 cut(s) 670, 786, 1329
RseI CAYNNNNRTG 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 5 cut(s) 111, 702, 762, 795, 804
SatI GCNGC 2 cut(s) 294, 339
Sau3AI GATC 8 cut(s) 223, 646, 705, 717, 1057, 1117, 1153, 1178
Sau96I GGNCC 3 cut(s) 270, 1305, 1306
SchI GAGTC 4 cut(s) 37, 425, 563, 1280
ScrFI CCNGG 4 cut(s) 203, 570, 714, 1115
SduI GDGCHC 1 cut(s) 1309
SfaNI GCATC 3 cut(s) 160, 517, 1081
SfcI CTRYAG 1 cut(s) 774
SfuI TTCGAA 1 cut(s) 1295
SinI GGWCC 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 562
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
SsiI CCGC 2 cut(s) 196, 1303
SspMI CTAG 2 cut(s) 744, 1415
StyD4I CCNGG 4 cut(s) 201, 568, 712, 1113
StyI CCWWGG 2 cut(s) 243, 1344
TaaI ACNGT 7 cut(s) 47, 152, 445, 790, 849, 1012, 1333
TaiI ACGT 3 cut(s) 301, 998, 1425
TaqI TCGA 3 cut(s) 843, 1006, 1295
TaqII GACCGA 1 cut(s) 1400
TasI AATT 7 cut(s) 276, 285, 894, 910, 941, 1291, 1320
TatI WGTACW 2 cut(s) 669, 1328
TfiI GAWTC 6 cut(s) 18, 548, 766, 979, 1003, 1145
Tru1I TTAA 5 cut(s) 111, 702, 762, 795, 804
Tru9I TTAA 5 cut(s) 111, 702, 762, 795, 804
TscAI CASTG 1 cut(s) 564
TseFI GTSAC 4 cut(s) 299, 555, 1042, 1109
TseI GCWGC 2 cut(s) 293, 338
Tsp45I GTSAC 4 cut(s) 299, 555, 1042, 1109
TspDTI ATGAA 6 cut(s) 408, 806, 829, 934, 952, 1302
TspRI CASTG 1 cut(s) 564
Van91I CCANNNNNTGG 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 894
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 2 cut(s) 744, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.