MD01G1236900.v1.1

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003548
Physical Location & Seq
Reverse (-)
32415678 .. 32417340
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1236900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGAGATAAGGACATTGAAAGTTAGCAATGTCTCCCTAGCTGCAACGGAACATGATTTAAAGGATTTCTTTTCCTTCTCCGGTGAAGTTGAATACATTGAAATTCAAGGCGACAACGAGAGGTCTCAGGTTGCATATGTTACCTTCAAGGATTTCAAGGGGGCAGAAACTTCAGTGCTTCTTTCGGGAGCAACAATTGTCAATCAGTCCGTTACCATAGATCTGGCTCCAGATTACAAGCTTCCAGCTGCTGTGGCTTCAGCACTTCCGACTGCAACGGAAAATGTAAGTCCTGGTGGTGTGCAATCTGCTGTACACAATGCAGAGGATGTCGTGAGCAGCATGCTCGCCAAAGGATTCATTTTGGGAAAAGATGCGATCAACAAGGCAAAGGCCTTTGATGAGAAACACCAGCTTAGTTCCACTGCGACGGCCACAGTTTCTGCTTTGGACCAAAAGATTGGTTTCACTGACAAACTTAATGCTGGCACAAGCATGGTAAATGATAAGGTGAAGCAAATGGATGAAAGGTTTCAGGTTTCTGAGAAGACCAAGGTTGTGTTTTCAGCTGCAGAGCAAACGGTCAGTAGTGCTGGATCTGCAATCATGAAGAATCATTACATATTAACTGGGACATCGTGGGTAACCGGTGCTTTTAATAGGGCTGCCAAGGCAGCAACTGAAGTGAGCCAAAAGACAATTGATAAGGTTTCAGCTCAGGAAGAGGGTGGAAGGAAAGTGGAAGGAAGTGGTCCGGTCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

255

Amino Acids

27.0

Weight (kDa)

5.6

Isoelectric Point (pI)

29.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 7 - 68 1e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 604
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 3 cut(s) 156, 243, 702
AfaI GTAC 1 cut(s) 315
AgeI ACCGGT 1 cut(s) 647
AgsI TTSAA 6 cut(s) 19, 92, 101, 107, 148, 157
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 6 cut(s) 41, 241, 248, 415, 569, 716
AluI AGCT 6 cut(s) 41, 241, 248, 415, 569, 716
Alw26I GTCTC 2 cut(s) 37, 129
AlwI GGATC 1 cut(s) 604
AlwNI CAGNNNCTG 3 cut(s) 251, 443, 680
AoxI GGCC 2 cut(s) 393, 432
ApeKI GCWGC 6 cut(s) 41, 248, 339, 569, 665, 674
ApoI RAATTY 1 cut(s) 102
AsiGI ACCGGT 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 531
AspS9I GGNCC 3 cut(s) 451, 752, 757
AsuHPI GGTGA 2 cut(s) 95, 523
AvaII GGWCC 3 cut(s) 451, 752, 757
BarI GAAGNNNNNNTAC 2 cut(s) 602, 634
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 6 cut(s) 28, 235, 351, 556, 652, 686
BceAI ACGGC 1 cut(s) 447
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 37, 129
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 570
BglII AGATCT 1 cut(s) 220
BisI GCNGC 6 cut(s) 42, 249, 340, 570, 666, 675
BlsI GCNGC 6 cut(s) 43, 250, 341, 571, 667, 676
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 3 cut(s) 451, 752, 757
BmgT120I GGNCC 3 cut(s) 451, 752, 757
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 294
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 552, 669
BsaWI WCCGGW 3 cut(s) 80, 647, 754
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 1 cut(s) 634
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 552, 669
BseGI GGATG 2 cut(s) 334, 529
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 3 cut(s) 140, 534, 731
BseNI ACTGG 1 cut(s) 634
BseXI GCAGC 6 cut(s) 28, 235, 351, 556, 652, 686
BshFI GGCC 2 cut(s) 395, 434
BshTI ACCGGT 1 cut(s) 647
BsiSI CCGG 3 cut(s) 81, 648, 755
BslFI GGGAC 1 cut(s) 646
BsmAI GTCTC 2 cut(s) 37, 129
BsmFI GGGAC 1 cut(s) 646
BsnI GGCC 2 cut(s) 395, 434
Bso31I GGTCTC 1 cut(s) 129
Bsp1407I TGTACA 1 cut(s) 313
Bsp143I GATC 3 cut(s) 220, 378, 596
BspANI GGCC 2 cut(s) 395, 434
BspCNI CTCAG 3 cut(s) 139, 535, 730
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 647
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 1 cut(s) 634
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 2 cut(s) 552, 669
BssMI GATC 3 cut(s) 220, 378, 596
BssT1I CCWWGG 2 cut(s) 552, 669
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 439, 583
Bst6I CTCTTC 1 cut(s) 717
BstAUI TGTACA 1 cut(s) 313
BstC8I GCNNGC 3 cut(s) 344, 348, 487
BstDEI CTNAG 4 cut(s) 126, 416, 543, 717
BstEII GGTNACC 1 cut(s) 643
BstF5I GGATG 2 cut(s) 334, 529
BstKTI GATC 3 cut(s) 223, 381, 599
BstMAI GTCTC 2 cut(s) 37, 129
BstMBI GATC 3 cut(s) 220, 378, 596
BstMWI GCNNNNNNNGC 4 cut(s) 254, 599, 671, 674
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 346
BstPI GGTNACC 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 292
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 6 cut(s) 28, 235, 351, 556, 652, 686
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 2 cut(s) 220, 596
BstXI CCANNNNNNTGG 2 cut(s) 223, 461
BstYI RGATCY 2 cut(s) 220, 596
BsuRI GGCC 2 cut(s) 395, 434
BtsCI GGATG 2 cut(s) 334, 529
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 3 cut(s) 180, 423, 468
Cac8I GCNNGC 3 cut(s) 344, 348, 487
CaiI CAGNNNCTG 3 cut(s) 251, 443, 680
CciI TCATGA 1 cut(s) 606
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 3 cut(s) 451, 752, 757
Csp6I GTAC 1 cut(s) 314
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 4 cut(s) 53, 343, 496, 607
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 4 cut(s) 126, 416, 543, 717
DpnI GATC 3 cut(s) 222, 380, 598
DpnII GATC 3 cut(s) 220, 378, 596
DraI TTTAAA 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco130I CCWWGG 2 cut(s) 552, 669
Eco147I AGGCCT 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 3 cut(s) 451, 752, 757
Eco57I CTGAAG 3 cut(s) 156, 243, 702
Eco91I GGTNACC 1 cut(s) 643
EcoO65I GGTNACC 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 552, 669
ErhI CCWWGG 2 cut(s) 552, 669
FaeI CATG 4 cut(s) 56, 346, 499, 610
FaiI YATR 8 cut(s) 54, 136, 138, 218, 344, 497, 608, 623
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 646
FatI CATG 4 cut(s) 52, 342, 495, 606
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 6 cut(s) 42, 249, 340, 570, 666, 675
FokI GGATG 2 cut(s) 341, 536
Fsp4HI GCNGC 6 cut(s) 42, 249, 340, 570, 666, 675
FspBI CTAG 1 cut(s) 38
GluI GCNGC 6 cut(s) 42, 249, 340, 570, 666, 675
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 2 cut(s) 395, 434
HapII CCGG 3 cut(s) 81, 648, 755
Hin1II CATG 4 cut(s) 56, 346, 499, 610
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 2 cut(s) 357, 613
HpaII CCGG 3 cut(s) 81, 648, 755
HphI GGTGA 2 cut(s) 95, 523
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 270, 544
Hpy188III TCNNGA 5 cut(s) 186, 230, 334, 607, 719
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 4 cut(s) 85, 154, 726, 737
HpyCH4III ACNGT 2 cut(s) 439, 583
HpyCH4V TGCA 7 cut(s) 44, 134, 275, 304, 323, 572, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 599, 671, 674
HpyF3I CTNAG 4 cut(s) 126, 416, 543, 717
Hsp92II CATG 4 cut(s) 56, 346, 499, 610
Kzo9I GATC 3 cut(s) 220, 378, 596
LmnI GCTCC 2 cut(s) 188, 232
Lsp1109I GCAGC 6 cut(s) 28, 235, 351, 556, 652, 686
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 139, 211, 643
MalI GATC 3 cut(s) 222, 380, 598
MboI GATC 3 cut(s) 220, 378, 596
MboII GAAGA 3 cut(s) 559, 622, 734
MfeI CAATTG 2 cut(s) 195, 699
MflI RGATCY 2 cut(s) 220, 596
MluCI AATT 3 cut(s) 102, 195, 699
MmeI TCCRAC 1 cut(s) 293
MnlI CCTC 3 cut(s) 114, 319, 718
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 4 cut(s) 59, 480, 626, 657
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 2 cut(s) 248, 569
MspI CCGG 3 cut(s) 81, 648, 755
MspR9I CCNGG 1 cut(s) 294
MunI CAATTG 2 cut(s) 195, 699
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 4 cut(s) 254, 599, 671, 674
NdeI CATATG 1 cut(s) 136
NdeII GATC 3 cut(s) 220, 378, 596
NlaIII CATG 4 cut(s) 56, 346, 499, 610
NlaIV GGNNCC 1 cut(s) 228
NspI RCATGY 1 cut(s) 346
PaeI GCATGC 1 cut(s) 346
PagI TCATGA 1 cut(s) 606
PceI AGGCCT 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 357, 613
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 6 cut(s) 43, 250, 341, 571, 667, 676
Psp6I CCWGG 1 cut(s) 292
PspEI GGTNACC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 3 cut(s) 451, 752, 757
PstI CTGCAG 1 cut(s) 574
PstNI CAGNNNCTG 3 cut(s) 251, 443, 680
PsuI RGATCY 2 cut(s) 220, 596
PvuII CAGCTG 2 cut(s) 248, 569
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 4 cut(s) 59, 480, 626, 657
SatI GCNGC 6 cut(s) 42, 249, 340, 570, 666, 675
Sau3AI GATC 3 cut(s) 220, 378, 596
Sau96I GGNCC 3 cut(s) 451, 752, 757
ScrFI CCNGG 1 cut(s) 294
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 3 cut(s) 451, 752, 757
SmiMI CAYNNNNRTG 1 cut(s) 494
SphI GCATGC 1 cut(s) 346
Sse9I AATT 3 cut(s) 102, 195, 699
SseBI AGGCCT 1 cut(s) 395
SspMI CTAG 1 cut(s) 38
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 2 cut(s) 552, 669
TaaI ACNGT 2 cut(s) 439, 583
TasI AATT 3 cut(s) 102, 195, 699
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 357, 613
Tru1I TTAA 4 cut(s) 59, 480, 626, 657
Tru9I TTAA 4 cut(s) 59, 480, 626, 657
TscAI CASTG 3 cut(s) 180, 430, 475
TseI GCWGC 6 cut(s) 41, 248, 339, 569, 665, 674
TspDTI ATGAA 3 cut(s) 349, 540, 623
TspGWI ACGGA 3 cut(s) 62, 199, 293
TspRI CASTG 3 cut(s) 180, 430, 475
VpaK11BI GGWCC 3 cut(s) 451, 752, 757
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.