MD07G1309500.v1.1

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003548
Physical Location & Seq
Reverse (-)
36475443 .. 36477651
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1309500.v1.1.491

Sequence Viewer

Length: 768 bp
ATGATGGAGATAAGGACGGTGAAAGTTAGCAATGTCTCCCTCGGTGCAACGGAACTTGATTTAAAGGACTTCTTTTCCTTCTCTGGTGATATTGAATACATTGAAATTCAAGGTGACAGCGAGAGGTCTCAGGTTGCATATGTAACCTTCAAGGATTTCAAGGGGGCAGAAACTTCAGTGCTTCTTTCGGGAGCAACAATTGTCGATCAGTCCGTTACCATAGATCTGGCTCCAGATTACAAGCTTCCAGCTGCTGTGGCTTCAGCACTTCCAACTGCAACAGAAAATTTTAGTCCTGTTGGTGCTCAACCTGCTGTTCACAACGCAGAGGATGTCGTGAGCAGCATGCTCGCCAAAGGATTCATTTTGGGAAAAGATGCGATCAACAAGGCAAAGGCCTTTGATGAGAAACACCAGCTTAGTTCCACTGCGACGGCCACAGTTTCTTCTTTGGACCAAAAGATTGGTTTCACTGATAAACTTAGTGCTGGCACAACCATGGTAAATGATAAGGTGAAGCAAATGGATGAAAGGTTTCAGGTTTCTGAGAAGACCAAGATTGCGTTTTCAGCTGCTGAGCAAACGGTGAGTAGTGCTGGATCTGCAATCATGCAGAATCGTTACGTATTGACTGGGACATCATGGGTAACCGATGCTTTTAGAAGGGCTGCCAGGGCGGCAACAGAAGTGGGCCAGAAGACGACGGAAAAAGTTTCAGCTCAGGAAGAGGGTGGAAGGAAAGTGGAAGGAAGTGATCCGGTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

256

Amino Acids

27.27

Weight (kDa)

5.03

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 8 - 67 6.7e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
AciI CCGC 1 cut(s) 677
AclWI GGATC 2 cut(s) 607, 749
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 105, 286
AcuI CTGAAG 2 cut(s) 159, 246
AgsI TTSAA 5 cut(s) 95, 104, 110, 151, 160
AjnI CCWGG 1 cut(s) 671
AluBI AGCT 5 cut(s) 244, 251, 418, 572, 719
AluI AGCT 5 cut(s) 244, 251, 418, 572, 719
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 2 cut(s) 40, 132
AlwI GGATC 2 cut(s) 607, 749
AlwNI CAGNNNCTG 2 cut(s) 254, 575
AoxI GGCC 3 cut(s) 396, 435, 691
ApeKI GCWGC 4 cut(s) 251, 342, 572, 668
ApoI RAATTY 2 cut(s) 105, 286
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 3 cut(s) 454, 691, 760
AsuHPI GGTGA 5 cut(s) 31, 98, 125, 526, 598
AvaII GGWCC 2 cut(s) 454, 760
BbsI GAAGAC 2 cut(s) 557, 704
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 4 cut(s) 238, 354, 559, 655
BceAI ACGGC 1 cut(s) 450
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 1 cut(s) 673
BcoDI GTCTC 2 cut(s) 40, 132
BfuAI ACCTGC 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 677
BglII AGATCT 1 cut(s) 223
BisI GCNGC 5 cut(s) 252, 343, 573, 669, 678
BlpI GCTNAGC 1 cut(s) 576
BlsI GCNGC 5 cut(s) 253, 344, 574, 670, 679
Bme1390I CCNGG 1 cut(s) 673
Bme18I GGWCC 2 cut(s) 454, 760
BmgT120I GGNCC 3 cut(s) 454, 691, 760
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 673
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 2 cut(s) 367, 643
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 2 cut(s) 557, 704
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 720
Bpu1102I GCTNAGC 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 40, 498, 672
BsaWI WCCGGW 1 cut(s) 757
Bse1I ACTGG 2 cut(s) 637, 763
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 673
BseDI CCNNGG 3 cut(s) 40, 498, 672
BseGI GGATG 2 cut(s) 337, 532
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 4 cut(s) 143, 537, 567, 734
BseNI ACTGG 2 cut(s) 637, 763
BseXI GCAGC 4 cut(s) 238, 354, 559, 655
BshFI GGCC 3 cut(s) 398, 437, 693
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 649
BsmAI GTCTC 2 cut(s) 40, 132
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 3 cut(s) 398, 437, 693
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 5 cut(s) 205, 223, 381, 599, 754
Bsp1720I GCTNAGC 1 cut(s) 576
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 1 cut(s) 677
BspANI GGCC 3 cut(s) 398, 437, 693
BspCNI CTCAG 4 cut(s) 142, 538, 568, 733
BspLI GGNNCC 1 cut(s) 231
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 2 cut(s) 607, 749
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 2 cut(s) 637, 763
BssECI CCNNGG 3 cut(s) 40, 498, 672
BssMI GATC 5 cut(s) 205, 223, 381, 599, 754
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 673
Bst4CI ACNGT 3 cut(s) 19, 442, 586
Bst6I CTCTTC 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 625
BstC8I GCNNGC 3 cut(s) 347, 351, 490
BstDEI CTNAG 6 cut(s) 129, 419, 482, 546, 576, 720
BstDSI CCRYGG 1 cut(s) 498
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 2 cut(s) 337, 532
BstKTI GATC 5 cut(s) 208, 226, 384, 602, 757
BstMAI GTCTC 2 cut(s) 40, 132
BstMBI GATC 5 cut(s) 205, 223, 381, 599, 754
BstMWI GCNNNNNNNGC 6 cut(s) 257, 311, 569, 602, 674, 677
BstNI CCWGG 1 cut(s) 673
BstNSI RCATGY 1 cut(s) 349
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 1 cut(s) 671
BstSNI TACGTA 1 cut(s) 625
BstV1I GCAGC 4 cut(s) 238, 354, 559, 655
BstV2I GAAGAC 2 cut(s) 557, 704
BstX2I RGATCY 2 cut(s) 223, 599
BstXI CCANNNNNNTGG 2 cut(s) 226, 464
BstYI RGATCY 2 cut(s) 223, 599
BsuRI GGCC 3 cut(s) 398, 437, 693
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 2 cut(s) 337, 532
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 3 cut(s) 183, 426, 471
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 3 cut(s) 347, 351, 490
CaiI CAGNNNCTG 2 cut(s) 254, 575
Cfr13I GGNCC 3 cut(s) 454, 691, 760
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviAII CATG 4 cut(s) 346, 499, 610, 642
DdeI CTNAG 6 cut(s) 129, 419, 482, 546, 576, 720
DpnI GATC 5 cut(s) 207, 225, 383, 601, 756
DpnII GATC 5 cut(s) 205, 223, 381, 599, 754
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco105I TACGTA 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 498
Eco147I AGGCCT 1 cut(s) 398
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 454, 760
Eco57I CTGAAG 2 cut(s) 159, 246
Eco91I GGTNACC 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 646
EcoRII CCWGG 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 4 cut(s) 349, 502, 613, 645
FaiI YATR 7 cut(s) 139, 141, 221, 347, 500, 611, 643
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 649
FatI CATG 4 cut(s) 345, 498, 609, 641
FauNDI CATATG 1 cut(s) 139
Fnu4HI GCNGC 5 cut(s) 252, 343, 573, 669, 678
FokI GGATG 2 cut(s) 344, 539
Fsp4HI GCNGC 5 cut(s) 252, 343, 573, 669, 678
GluI GCNGC 5 cut(s) 252, 343, 573, 669, 678
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 3 cut(s) 398, 437, 693
HapII CCGG 1 cut(s) 758
Hin1II CATG 4 cut(s) 349, 502, 613, 645
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 2 cut(s) 360, 616
HpaII CCGG 1 cut(s) 758
HphI GGTGA 5 cut(s) 31, 98, 125, 526, 598
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 547
Hpy188III TCNNGA 4 cut(s) 189, 233, 337, 722
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 2 cut(s) 436, 706
HpyAV CCTTC 5 cut(s) 88, 157, 657, 729, 740
HpyCH4III ACNGT 3 cut(s) 19, 442, 586
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 5 cut(s) 47, 137, 278, 605, 613
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 311, 569, 602, 674, 677
HpyF3I CTNAG 6 cut(s) 129, 419, 482, 546, 576, 720
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 4 cut(s) 349, 502, 613, 645
Kzo9I GATC 5 cut(s) 205, 223, 381, 599, 754
LmnI GCTCC 2 cut(s) 191, 235
Lsp1109I GCAGC 4 cut(s) 238, 354, 559, 655
LweI GCATC 2 cut(s) 367, 643
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 5 cut(s) 113, 142, 214, 620, 646
MalI GATC 5 cut(s) 207, 225, 383, 601, 756
MboI GATC 5 cut(s) 205, 223, 381, 599, 754
MboII GAAGA 4 cut(s) 438, 562, 709, 737
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 2 cut(s) 223, 599
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 3 cut(s) 105, 198, 286
MmeI TCCRAC 1 cut(s) 296
MnlI CCTC 4 cut(s) 50, 117, 322, 721
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 2 cut(s) 251, 572
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 673
MunI CAATTG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 673
MwoI GCNNNNNNNGC 6 cut(s) 257, 311, 569, 602, 674, 677
NcoI CCATGG 1 cut(s) 498
NdeI CATATG 1 cut(s) 139
NdeII GATC 5 cut(s) 205, 223, 381, 599, 754
NlaIII CATG 4 cut(s) 349, 502, 613, 645
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 349
PaeI GCATGC 1 cut(s) 349
PceI AGGCCT 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 2 cut(s) 360, 616
PkrI GCNGC 5 cut(s) 253, 344, 574, 670, 679
Ppu21I YACGTR 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 671
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 671
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 3 cut(s) 454, 691, 760
PstNI CAGNNNCTG 2 cut(s) 254, 575
PsuI RGATCY 2 cut(s) 223, 599
PvuII CAGCTG 2 cut(s) 251, 572
RseI CAYNNNNRTG 1 cut(s) 497
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 5 cut(s) 252, 343, 573, 669, 678
Sau3AI GATC 5 cut(s) 205, 223, 381, 599, 754
Sau96I GGNCC 3 cut(s) 454, 691, 760
ScrFI CCNGG 1 cut(s) 673
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 2 cut(s) 367, 643
SinI GGWCC 2 cut(s) 454, 760
SmiMI CAYNNNNRTG 1 cut(s) 497
SnaBI TACGTA 1 cut(s) 625
SphI GCATGC 1 cut(s) 349
Sse9I AATT 3 cut(s) 105, 198, 286
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 1 cut(s) 677
StuI AGGCCT 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 671
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 3 cut(s) 19, 442, 586
TaiI ACGT 1 cut(s) 627
TaqI TCGA 1 cut(s) 204
TasI AATT 3 cut(s) 105, 198, 286
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 2 cut(s) 360, 616
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 3 cut(s) 183, 433, 478
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 4 cut(s) 251, 342, 572, 668
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 352, 543
TspGWI ACGGA 3 cut(s) 65, 202, 719
TspRI CASTG 3 cut(s) 183, 433, 478
VpaK11BI GGWCC 2 cut(s) 454, 760
XapI RAATTY 2 cut(s) 105, 286
XceI RCATGY 1 cut(s) 349
XmnI GAANNNNTTC 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.