MD06G1025100.v1.1

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003548
Physical Location & Seq
Forward (+)
3045002 .. 3048250
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1025100.v1.1.491

Sequence Viewer

Length: 840 bp
ATGTCGGTAACAACCATCAAGGTCAGCAATCTTTCCTTGGGTGCAAGTGAGAGAGATATAAAGGAGTTCTTTTGTTTTTCTGGTGATATTGCTTATGTCGAAATGATGAGTGATACTGAGCGGTCCCAAATTGCATATGTTACCTTCAAGGATGTACAAGGAGCTGAGACTGCAGTTCTCCTTTCGGGAGCTACAATAGTGGATATGTCTGTCACCATAACTCTGGCTCCAGATTACGAGTTGCCACCTGATGCTATAGCACCAAATGTAACAGAGAATAAAACTCCTGGTGGGGGTGAATCTGCATTCCGCAAGGCAGAGGATGTTGTTAGTGGCATGCTTGCCAAGGGCTTTCTCTTAGGCAAAGATGCAGTCAACAGGGCAAAGGGCTTTGATGAGAAACACCAGTTGACCTCAACGGCTTCAGAAAAAGTTGCTTCTTTTGACCAAAAGATTGGTCTCAGCGAGAAAATAAGTGCTGGTACTTCTGCGGTCAGTGGCAAAGTTCGCGAAGTGGATCAGAAATTTCAGGTCTCAGAGAAAACCAAATCAGCTTTTGCAGCTGCTGAGCAGACAGTCAGTAGCGCTGGATCTGCTATAATGAAGAACCGGTATGTGTTTACCAGCGCTGCATGGGTTACAGGTGCTTTTAATAAGGTTGCAAAAGCAGCTGAAGAGGTTGGTCAGAAAACACAAGAGAAAGTTGGAAAGGTTGAAGAAGAGAAACATAGAAAAATAGAGGACGACTACGCTCAAGTTCTATCGGATTCTCCTAAAGCTTCTGCAGCAACCCAGCACGATCCCTCCAAGCCTGAACCTGTCAAAGGTTTGATCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

280

Amino Acids

29.8

Weight (kDa)

5.45

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 7 - 66 2.4e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 121
AccII CGCG 1 cut(s) 510
AciI CCGC 3 cut(s) 121, 310, 491
AclWI GGATC 4 cut(s) 525, 598, 794, 826
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 408, 693
AfaI GTAC 2 cut(s) 156, 484
AfeI AGCGCT 2 cut(s) 586, 628
AfiI CCNNNNNNNGG 2 cut(s) 293, 824
AgeI ACCGGT 1 cut(s) 609
AgsI TTSAA 2 cut(s) 148, 716
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 6 cut(s) 164, 191, 554, 563, 671, 779
AluI AGCT 6 cut(s) 164, 191, 554, 563, 671, 779
Alw26I GTCTC 3 cut(s) 161, 464, 538
AlwI GGATC 4 cut(s) 525, 598, 794, 826
AlwNI CAGNNNCTG 1 cut(s) 566
Aor51HI AGCGCT 2 cut(s) 586, 628
ApeKI GCWGC 5 cut(s) 560, 563, 629, 668, 785
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 4 cut(s) 357, 389, 442, 474
AsiGI ACCGGT 1 cut(s) 609
AspLEI GCGC 2 cut(s) 587, 629
AspS9I GGNCC 1 cut(s) 123
AsuHPI GGTGA 3 cut(s) 95, 205, 308
AvaII GGWCC 1 cut(s) 123
BbvI GCAGC 5 cut(s) 550, 572, 616, 680, 797
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 288
BcoDI GTCTC 3 cut(s) 161, 464, 538
BfmI CTRYAG 3 cut(s) 171, 255, 783
BfoI RGCGCY 2 cut(s) 588, 630
BisI GCNGC 5 cut(s) 561, 564, 630, 669, 786
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 5 cut(s) 562, 565, 631, 670, 787
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 2 cut(s) 125, 228
BmrFI CCNGG 1 cut(s) 288
BmsI GCATC 2 cut(s) 241, 358
BpmI CTGGAG 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 464, 538
BsaJI CCNNGG 2 cut(s) 36, 345
BsaWI WCCGGW 1 cut(s) 609
BsaXI ACNNNNNCTCC 4 cut(s) 211, 241, 788, 818
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 824
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 36, 345
BseGI GGATG 2 cut(s) 157, 328
BseLI CCNNNNNNNGG 2 cut(s) 293, 824
BseMII CTCAG 5 cut(s) 108, 156, 475, 549, 558
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 5 cut(s) 550, 572, 616, 680, 797
BseYI CCCAGC 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 609
BsiSI CCGG 1 cut(s) 610
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 2 cut(s) 293, 824
BsmAI GTCTC 3 cut(s) 161, 464, 538
BsmFI GGGAC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 305
Bso31I GGTCTC 2 cut(s) 464, 538
Bsp1407I TGTACA 1 cut(s) 154
Bsp143I GATC 4 cut(s) 517, 590, 799, 831
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 3 cut(s) 121, 310, 491
BspCNI CTCAG 5 cut(s) 109, 157, 474, 548, 559
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 125, 228
BspMAI CTGCAG 2 cut(s) 175, 787
BspPI GGATC 4 cut(s) 525, 598, 794, 826
BspTNI GGTCTC 2 cut(s) 464, 538
BsrBI CCGCTC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 609
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 36, 345
BssMI GATC 4 cut(s) 517, 590, 799, 831
BssT1I CCWWGG 2 cut(s) 36, 345
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 2 cut(s) 669, 714
BstAUI TGTACA 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 338, 342
BstDEI CTNAG 6 cut(s) 117, 165, 358, 461, 535, 567
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 2 cut(s) 157, 328
BstFNI CGCG 1 cut(s) 510
BstH2I RGCGCY 2 cut(s) 588, 630
BstHHI GCGC 2 cut(s) 587, 629
BstKTI GATC 4 cut(s) 520, 593, 802, 834
BstMAI GTCTC 3 cut(s) 161, 464, 538
BstMBI GATC 4 cut(s) 517, 590, 799, 831
BstMWI GCNNNNNNNGC 6 cut(s) 170, 507, 560, 593, 668, 785
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 3 cut(s) 171, 255, 783
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 5 cut(s) 550, 572, 616, 680, 797
BstX2I RGATCY 1 cut(s) 590
BstXI CCANNNNNNTGG 2 cut(s) 223, 455
BstYI RGATCY 1 cut(s) 590
BtsCI GGATG 2 cut(s) 157, 328
BtsIMutI CAGTG 1 cut(s) 502
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 338, 342
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 2 cut(s) 587, 629
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 1 cut(s) 123
Csp6I GTAC 2 cut(s) 155, 483
CspAI ACCGGT 1 cut(s) 609
CviAII CATG 2 cut(s) 337, 633
CviQI GTAC 2 cut(s) 155, 483
DdeI CTNAG 6 cut(s) 117, 165, 358, 461, 535, 567
DpnI GATC 4 cut(s) 519, 592, 801, 833
DpnII GATC 4 cut(s) 517, 590, 799, 831
Eam1104I CTCTTC 2 cut(s) 669, 714
EarI CTCTTC 2 cut(s) 669, 714
Eco130I CCWWGG 2 cut(s) 36, 345
Eco31I GGTCTC 2 cut(s) 464, 538
Eco47I GGWCC 1 cut(s) 123
Eco47III AGCGCT 2 cut(s) 586, 628
Eco57I CTGAAG 2 cut(s) 408, 693
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 36, 345
ErhI CCWWGG 2 cut(s) 36, 345
FaeI CATG 2 cut(s) 340, 636
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 109
FatI CATG 2 cut(s) 336, 632
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 5 cut(s) 561, 564, 630, 669, 786
FokI GGATG 2 cut(s) 164, 335
Fsp4HI GCNGC 5 cut(s) 561, 564, 630, 669, 786
GlaI GCGC 2 cut(s) 586, 628
GluI GCNGC 5 cut(s) 561, 564, 630, 669, 786
GsaI CCCAGC 1 cut(s) 796
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 2 cut(s) 588, 630
HapII CCGG 1 cut(s) 610
HhaI GCGC 2 cut(s) 587, 629
Hin1II CATG 2 cut(s) 340, 636
Hin6I GCGC 2 cut(s) 585, 627
HinP1I GCGC 2 cut(s) 585, 627
HincII GTYRAC 2 cut(s) 376, 411
HindII GTYRAC 2 cut(s) 376, 411
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 2 cut(s) 299, 767
HpaII CCGG 1 cut(s) 610
HphI GGTGA 3 cut(s) 95, 205, 308
Hpy166II GTNNAC 3 cut(s) 376, 411, 621
Hpy188I TCNGA 5 cut(s) 427, 522, 538, 687, 766
Hpy188III TCNNGA 3 cut(s) 186, 230, 509
Hpy8I GTNNAC 3 cut(s) 376, 411, 621
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4V TGCA 9 cut(s) 44, 134, 173, 305, 371, 560, 632, 662, 785
HpyF10VI GCNNNNNNNGC 6 cut(s) 170, 507, 560, 593, 668, 785
HpyF3I CTNAG 6 cut(s) 117, 165, 358, 461, 535, 567
Hsp92II CATG 2 cut(s) 340, 636
HspAI GCGC 2 cut(s) 585, 627
Kzo9I GATC 4 cut(s) 517, 590, 799, 831
LmnI GCTCC 3 cut(s) 161, 188, 232
Lsp1109I GCAGC 5 cut(s) 550, 572, 616, 680, 797
LweI GCATC 2 cut(s) 241, 358
MaeIII GTNAC 5 cut(s) 7, 139, 211, 268, 637
MalI GATC 4 cut(s) 519, 592, 801, 833
MbiI CCGCTC 1 cut(s) 121
MboI GATC 4 cut(s) 517, 590, 799, 831
MboII GAAGA 4 cut(s) 616, 686, 728, 731
MflI RGATCY 1 cut(s) 590
MluCI AATT 2 cut(s) 129, 524
MmeI TCCRAC 1 cut(s) 685
MnlI CCTC 5 cut(s) 313, 424, 670, 733, 814
MseI TTAA 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 563, 671
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 6 cut(s) 170, 507, 560, 593, 668, 785
NdeI CATATG 1 cut(s) 136
NdeII GATC 4 cut(s) 517, 590, 799, 831
NlaIII CATG 2 cut(s) 340, 636
NlaIV GGNNCC 2 cut(s) 125, 228
NmuCI GTSAC 1 cut(s) 211
NruI TCGCGA 1 cut(s) 510
NspI RCATGY 1 cut(s) 340
PaeI GCATGC 1 cut(s) 340
PctI GAATGC 1 cut(s) 305
PfeI GAWTC 2 cut(s) 299, 767
PinAI ACCGGT 1 cut(s) 609
PkrI GCNGC 5 cut(s) 562, 565, 631, 670, 787
Psp6I CCWGG 1 cut(s) 286
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 2 cut(s) 125, 228
PspPI GGNCC 1 cut(s) 123
PstI CTGCAG 2 cut(s) 175, 787
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 590
PvuII CAGCTG 2 cut(s) 563, 671
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 2 cut(s) 156, 484
RsaNI GTAC 2 cut(s) 155, 483
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 5 cut(s) 561, 564, 630, 669, 786
Sau3AI GATC 4 cut(s) 517, 590, 799, 831
Sau96I GGNCC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 288
SfaNI GCATC 2 cut(s) 241, 358
SfcI CTRYAG 3 cut(s) 171, 255, 783
SinI GGWCC 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
SphI GCATGC 1 cut(s) 340
Sse9I AATT 2 cut(s) 129, 524
SsiI CCGC 3 cut(s) 121, 310, 491
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 2 cut(s) 36, 345
TaaI ACNGT 1 cut(s) 577
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 129, 524
TatI WGTACW 1 cut(s) 154
TfiI GAWTC 2 cut(s) 299, 767
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 5 cut(s) 560, 563, 629, 668, 785
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 617
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 123
XagI CCTNNNNNAGG 1 cut(s) 822
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.