MD06G1025200.v1.1

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003548
Physical Location & Seq
Forward (+)
3052051 .. 3055407
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1025200.v1.1.491

Sequence Viewer

Length: 840 bp
ATGTCGGTAACAACCATCAAGGTCAGCAATCTTTCCTTGGGTGCAAGTGAGAGAGACATAAAGGAGTTCTTTTCTTTTTCTGGTGATATTGCTTATGTCGAAATGATGAGTGATACTGAGCGATCCCAAATTGCATATGTTACCTTCAAAGATGTACAAGGAGCTGAGACTGCAGTTCTTCTTTCGGGAGCTACAATAGTGGATATGTCTGTCAGCATAACTCTGGCTCCAGATTACGAGTTGCCACCTGATGCTATAGCACCAAGTGCAACAGAGAATAAAACTCCTGGTGGGGATGAATCTGCATTCCGGAAGGCAGAGGATGTTGTTAGTGGCATGCTTGCTAAGGGCTTTCTCTTGGGCAAAGATGCAGTCAACAAGGCAAAGGGCTTTGATGAGAAACACCAATTGACCTCAACGGCTTCAGAAAAAGTTGCTTCCTTTGACAAAAAGATTGGTCTCAGCGAGAAAATAAGTGCTGGTACTTCTGCGGTCAGTGGCAAAGTTCGCGAAGTGGATCAGAAATTCCAGGTCTCAGAGAAAACCAAATCAGCTTTTGCAGCTGCTGAGCAGACGGTCAGTAGCGCTGGATCTGCTATAATGAAGAACCGGTATGTGTTTACTAGCGCTCAATGGGTTACAGGTGCTTTTAATAAGGTTGCAAAAGCAGCTGAAGAGGTTGGTCAGAAAACAAAAGAGAAAGTTGGAAAGGTTGAAGAAGAGAAACATAGAAAAATAGAGGATGACTATGCTCAAGTTCTATCGGATTCTCCTAAAGCTTCTGCAGCAACCCAGCACGATCCCTCCAAGCCTGAACCCGTTAAAGGTTTGATCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

280

Amino Acids

29.8

Weight (kDa)

5.61

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 7 - 66 1.3e-08 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 309
AciI CCGC 1 cut(s) 491
AclWI GGATC 5 cut(s) 117, 525, 598, 794, 826
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 408, 693
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 2 cut(s) 156, 484
AfeI AGCGCT 2 cut(s) 586, 628
AfiI CCNNNNNNNGG 1 cut(s) 824
AgeI ACCGGT 1 cut(s) 609
AgsI TTSAA 2 cut(s) 148, 716
AjnI CCWGG 2 cut(s) 286, 528
AluBI AGCT 6 cut(s) 164, 191, 554, 563, 671, 779
AluI AGCT 6 cut(s) 164, 191, 554, 563, 671, 779
Alw26I GTCTC 4 cut(s) 48, 161, 464, 538
AlwI GGATC 5 cut(s) 117, 525, 598, 794, 826
AlwNI CAGNNNCTG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 309
Aor51HI AGCGCT 2 cut(s) 586, 628
ApeKI GCWGC 4 cut(s) 560, 563, 668, 785
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AsiGI ACCGGT 1 cut(s) 609
AspLEI GCGC 2 cut(s) 587, 629
AsuHPI GGTGA 1 cut(s) 95
BbvI GCAGC 4 cut(s) 550, 572, 680, 797
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 2 cut(s) 288, 530
BcoDI GTCTC 4 cut(s) 48, 161, 464, 538
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 3 cut(s) 171, 255, 783
BfoI RGCGCY 2 cut(s) 588, 630
BisI GCNGC 4 cut(s) 561, 564, 669, 786
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 4 cut(s) 562, 565, 670, 787
Bme1390I CCNGG 2 cut(s) 288, 530
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 288, 530
BmsI GCATC 2 cut(s) 241, 358
BpmI CTGGAG 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 464, 538
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 2 cut(s) 309, 609
BsaXI ACNNNNNCTCC 4 cut(s) 211, 241, 788, 818
Bsc4I CCNNNNNNNGG 1 cut(s) 824
Bse118I RCCGGY 1 cut(s) 609
BseAI TCCGGA 1 cut(s) 309
BseBI CCWGG 2 cut(s) 288, 530
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 3 cut(s) 301, 328, 748
BseLI CCNNNNNNNGG 1 cut(s) 824
BseMII CTCAG 5 cut(s) 108, 156, 475, 549, 558
BseXI GCAGC 4 cut(s) 550, 572, 680, 797
BseYI CCCAGC 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 609
BsiSI CCGG 2 cut(s) 310, 610
BslI CCNNNNNNNGG 1 cut(s) 824
BsmAI GTCTC 4 cut(s) 48, 161, 464, 538
BsmI GAATGC 1 cut(s) 305
Bso31I GGTCTC 2 cut(s) 464, 538
Bsp13I TCCGGA 1 cut(s) 309
Bsp1407I TGTACA 1 cut(s) 154
Bsp143I GATC 5 cut(s) 122, 517, 590, 799, 831
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 1 cut(s) 491
BspCNI CTCAG 5 cut(s) 109, 157, 474, 548, 559
BspEI TCCGGA 1 cut(s) 309
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 2 cut(s) 175, 787
BspPI GGATC 5 cut(s) 117, 525, 598, 794, 826
BspTNI GGTCTC 2 cut(s) 464, 538
BsrFI RCCGGY 1 cut(s) 609
BsrGI TGTACA 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 5 cut(s) 122, 517, 590, 799, 831
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 288, 530
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 2 cut(s) 669, 714
BstAPI GCANNNNNTGC 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 338, 342
BstDEI CTNAG 6 cut(s) 117, 165, 345, 461, 535, 567
BstF5I GGATG 3 cut(s) 301, 328, 748
BstFNI CGCG 1 cut(s) 510
BstH2I RGCGCY 2 cut(s) 588, 630
BstHHI GCGC 2 cut(s) 587, 629
BstKTI GATC 5 cut(s) 125, 520, 593, 802, 834
BstMAI GTCTC 4 cut(s) 48, 161, 464, 538
BstMBI GATC 5 cut(s) 122, 517, 590, 799, 831
BstMWI GCNNNNNNNGC 7 cut(s) 170, 266, 507, 560, 593, 668, 785
BstNI CCWGG 2 cut(s) 288, 530
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 286, 528
BstSFI CTRYAG 3 cut(s) 171, 255, 783
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 550, 572, 680, 797
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BtsCI GGATG 3 cut(s) 301, 328, 748
BtsIMutI CAGTG 1 cut(s) 502
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 338, 342
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 2 cut(s) 587, 629
Cfr10I RCCGGY 1 cut(s) 609
Csp6I GTAC 2 cut(s) 155, 483
CspAI ACCGGT 1 cut(s) 609
CviAII CATG 1 cut(s) 337
CviQI GTAC 2 cut(s) 155, 483
DdeI CTNAG 6 cut(s) 117, 165, 345, 461, 535, 567
DpnI GATC 5 cut(s) 124, 519, 592, 801, 833
DpnII GATC 5 cut(s) 122, 517, 590, 799, 831
DraIII CACNNNGTG 1 cut(s) 266
Eam1104I CTCTTC 2 cut(s) 669, 714
EarI CTCTTC 2 cut(s) 669, 714
Eco130I CCWWGG 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 464, 538
Eco47III AGCGCT 2 cut(s) 586, 628
Eco57I CTGAAG 2 cut(s) 408, 693
EcoRII CCWGG 2 cut(s) 286, 528
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 1 cut(s) 340
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 1 cut(s) 336
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 4 cut(s) 561, 564, 669, 786
FokI GGATG 3 cut(s) 308, 335, 755
Fsp4HI GCNGC 4 cut(s) 561, 564, 669, 786
FspBI CTAG 1 cut(s) 624
GlaI GCGC 2 cut(s) 586, 628
GluI GCNGC 4 cut(s) 561, 564, 669, 786
GsaI CCCAGC 1 cut(s) 796
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 2 cut(s) 588, 630
HapII CCGG 2 cut(s) 310, 610
HhaI GCGC 2 cut(s) 587, 629
Hin1II CATG 1 cut(s) 340
Hin6I GCGC 2 cut(s) 585, 627
HinP1I GCGC 2 cut(s) 585, 627
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 2 cut(s) 299, 767
HpaII CCGG 2 cut(s) 310, 610
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 376, 621
Hpy188I TCNGA 6 cut(s) 427, 522, 538, 687, 766, 839
Hpy188III TCNNGA 4 cut(s) 186, 230, 310, 509
Hpy8I GTNNAC 2 cut(s) 376, 621
HpyAV CCTTC 2 cut(s) 154, 307
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4V TGCA 9 cut(s) 44, 134, 173, 269, 305, 371, 560, 662, 785
HpyF10VI GCNNNNNNNGC 7 cut(s) 170, 266, 507, 560, 593, 668, 785
HpyF3I CTNAG 6 cut(s) 117, 165, 345, 461, 535, 567
Hsp92II CATG 1 cut(s) 340
HspAI GCGC 2 cut(s) 585, 627
Kpn2I TCCGGA 1 cut(s) 309
Kzo9I GATC 5 cut(s) 122, 517, 590, 799, 831
LmnI GCTCC 3 cut(s) 161, 188, 232
Lsp1109I GCAGC 4 cut(s) 550, 572, 680, 797
LweI GCATC 2 cut(s) 241, 358
MaeI CTAG 1 cut(s) 624
MaeIII GTNAC 3 cut(s) 7, 139, 637
MalI GATC 5 cut(s) 124, 519, 592, 801, 833
MboI GATC 5 cut(s) 122, 517, 590, 799, 831
MboII GAAGA 5 cut(s) 170, 616, 686, 728, 731
MfeI CAATTG 1 cut(s) 407
MflI RGATCY 1 cut(s) 590
MluCI AATT 3 cut(s) 129, 407, 524
MmeI TCCRAC 1 cut(s) 685
MnlI CCTC 5 cut(s) 313, 424, 670, 733, 814
MroI TCCGGA 1 cut(s) 309
MseI TTAA 2 cut(s) 651, 822
MspA1I CMGCKG 2 cut(s) 563, 671
MspI CCGG 2 cut(s) 310, 610
MspR9I CCNGG 2 cut(s) 288, 530
MunI CAATTG 1 cut(s) 407
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 2 cut(s) 288, 530
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 7 cut(s) 170, 266, 507, 560, 593, 668, 785
NdeI CATATG 1 cut(s) 136
NdeII GATC 5 cut(s) 122, 517, 590, 799, 831
NlaIII CATG 1 cut(s) 340
NlaIV GGNNCC 1 cut(s) 228
NruI TCGCGA 1 cut(s) 510
NspI RCATGY 1 cut(s) 340
PaeI GCATGC 1 cut(s) 340
PctI GAATGC 1 cut(s) 305
PfeI GAWTC 2 cut(s) 299, 767
PinAI ACCGGT 1 cut(s) 609
PkrI GCNGC 4 cut(s) 562, 565, 670, 787
Psp6I CCWGG 2 cut(s) 286, 528
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 2 cut(s) 286, 528
PspN4I GGNNCC 1 cut(s) 228
PstI CTGCAG 2 cut(s) 175, 787
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 590
PvuII CAGCTG 2 cut(s) 563, 671
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 2 cut(s) 156, 484
RsaNI GTAC 2 cut(s) 155, 483
SaqAI TTAA 2 cut(s) 651, 822
SatI GCNGC 4 cut(s) 561, 564, 669, 786
Sau3AI GATC 5 cut(s) 122, 517, 590, 799, 831
ScrFI CCNGG 2 cut(s) 288, 530
SfaNI GCATC 2 cut(s) 241, 358
SfcI CTRYAG 3 cut(s) 171, 255, 783
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
SphI GCATGC 1 cut(s) 340
Sse9I AATT 3 cut(s) 129, 407, 524
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 2 cut(s) 286, 528
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 577
TaqI TCGA 1 cut(s) 99
TasI AATT 3 cut(s) 129, 407, 524
TatI WGTACW 1 cut(s) 154
TfiI GAWTC 2 cut(s) 299, 767
Tru1I TTAA 2 cut(s) 651, 822
Tru9I TTAA 2 cut(s) 651, 822
TscAI CASTG 1 cut(s) 502
TseI GCWGC 4 cut(s) 560, 563, 668, 785
TspDTI ATGAA 2 cut(s) 312, 617
TspRI CASTG 1 cut(s) 502
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 340
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.