MD02G1030900.v1.1

Ethylene-responsive transcription factor WIN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003109
Physical Location & Seq
Reverse (-)
2411213 .. 2412459
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1030900.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGTGAAATCGAAAAAGTACAGAGGCGTCAGGCAGCGCCACTGGGGCTCTTGGGTCTCAGAAATCCGCCACCCCTTACTGAAGAGAAGGGTGTGGCTAGGCACGTTTGAGACAGCTGAAGAAGCAGCCCGAGCATACGATGAAGCTTCCGTTTTGATGAGCGGACGAAATGCCAAGACCAATTTCCCAATAATAACTTCTACAACTCAGAGTAATGGTACCCGTACCACAGTAGGATCATCAGATCACCCAAAAACTAGTAGCGGCGACTTGGATTCACGATCCGAACAGAAGGGTCTGTCGGAAATCTTGCATGCCAAGCTAAGGAAATGCGGCAAGATACCATCTCCATCCATGACCTGCTTGAGGCTCGACAATGAGAGCTCTCATATAGGAGTATGGCAAAAACGTGCAGGTCAACGCTCTGATAACTCCAATTGGGTCATGACTGTTCCACTTGGCAAGAAGAAGAATAATAGTACTGTTGATACTAATAATGCTGATGATCAGAGTGTTCTGTCATCACAATTTATGTCGAATTCAGATCAATCTGCTTCGATTGAAACATCATCAGAGAGGCCCCCCCAACTTATGGCGGAGATGGATGAAGAAGAGAAAATTGCGTTGCAGATGATAGAAGAGCTGCTTAACGGAAATTGTGCAAGTTCGGATTTATCATTTGGCATTCAACAAGGGGAGGAAAGAATTTATTTGTAG

Protein Analysis

239

Amino Acids

26.54

Weight (kDa)

7.74

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 6 - 49 3.3e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 368, 404
Acc65I GGTACC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 162
AciI CCGC 5 cut(s) 67, 162, 264, 333, 596
AclWI GGATC 2 cut(s) 244, 276
AcsI RAATTY 2 cut(s) 538, 705
AcuI CTGAAG 2 cut(s) 101, 138
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 4 cut(s) 20, 220, 226, 481
AfiI CCNNNNNNNGG 3 cut(s) 324, 366, 592
AgsI TTSAA 2 cut(s) 563, 689
AhlI ACTAGT 1 cut(s) 257
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 181, 199, 213
AluBI AGCT 5 cut(s) 116, 146, 322, 384, 643
AluI AGCT 5 cut(s) 116, 146, 322, 384, 643
Alw21I GWGCWC 1 cut(s) 386
Alw26I GTCTC 2 cut(s) 61, 104
AlwI GGATC 2 cut(s) 244, 276
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 3 cut(s) 34, 125, 643
ApoI RAATTY 2 cut(s) 538, 705
Asp718I GGTACC 1 cut(s) 218
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 579
AsuHPI GGTGA 2 cut(s) 16, 239
AvaI CYCGRG 1 cut(s) 129
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 50, 386
Bbv12I GWGCWC 1 cut(s) 386
BbvI GCAGC 3 cut(s) 46, 137, 630
BccI CCATC 3 cut(s) 352, 358, 595
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 61, 104
BcuI ACTAGT 1 cut(s) 257
BfaI CTAG 2 cut(s) 98, 258
BfoI RGCGCY 1 cut(s) 40
BfuAI ACCTGC 2 cut(s) 368, 404
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 5 cut(s) 35, 126, 265, 334, 644
BlsI GCNGC 5 cut(s) 36, 127, 266, 335, 645
BmcAI AGTACT 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 579
BmiI GGNNCC 2 cut(s) 220, 581
BmrI ACTGGG 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 52
Bpu10I CCTNAGC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 385
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 61
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 366, 592
Bse1I ACTGG 1 cut(s) 47
BseGI GGATG 2 cut(s) 350, 610
BseLI CCNNNNNNNGG 3 cut(s) 324, 366, 592
BseMII CTCAG 2 cut(s) 72, 221
BseNI ACTGG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 46, 137, 630
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 1 cut(s) 580
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 129
BslI CCNNNNNNNGG 3 cut(s) 324, 366, 592
BsmAI GTCTC 2 cut(s) 61, 104
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 1 cut(s) 580
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 129
Bsp1286I GDGCHC 2 cut(s) 50, 386
Bsp143I GATC 5 cut(s) 236, 244, 281, 505, 544
BspACI CCGC 5 cut(s) 67, 162, 264, 333, 596
BspANI GGCC 1 cut(s) 580
BspCNI CTCAG 2 cut(s) 71, 220
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 220, 581
BspMI ACCTGC 2 cut(s) 368, 404
BspPI GGATC 2 cut(s) 244, 276
BspQI GCTCTTC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 162
BsrI ACTGG 1 cut(s) 47
BssMI GATC 5 cut(s) 236, 244, 281, 505, 544
BssNI GRCGYC 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 232, 451, 484
Bst6I CTCTTC 3 cut(s) 77, 606, 633
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 3 cut(s) 58, 207, 323
BstENI CCTNNNNNAGG 1 cut(s) 364
BstF5I GGATG 2 cut(s) 350, 610
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 5 cut(s) 239, 247, 284, 508, 547
BstMAI GTCTC 2 cut(s) 61, 104
BstMBI GATC 5 cut(s) 236, 244, 281, 505, 544
BstMWI GCNNNNNNNGC 4 cut(s) 45, 122, 131, 319
BstNSI RCATGY 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 46, 137, 630
BsuRI GGCC 1 cut(s) 580
BtsCI GGATG 2 cut(s) 350, 610
BtsIMutI CAGTG 1 cut(s) 40
BveI ACCTGC 2 cut(s) 368, 404
Cac8I GCNNGC 1 cut(s) 315
CciI TCATGA 1 cut(s) 444
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 579
CseI GACGC 1 cut(s) 16
Csp6I GTAC 4 cut(s) 19, 219, 225, 480
CviAII CATG 3 cut(s) 314, 355, 445
CviQI GTAC 4 cut(s) 19, 219, 225, 480
DdeI CTNAG 3 cut(s) 58, 207, 323
DpnI GATC 5 cut(s) 238, 246, 283, 507, 546
DpnII GATC 5 cut(s) 236, 244, 281, 505, 544
Eam1104I CTCTTC 3 cut(s) 77, 606, 633
EarI CTCTTC 3 cut(s) 77, 606, 633
EciI GGCGGA 2 cut(s) 56, 611
Ecl136II GAGCTC 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 50, 386
Eco31I GGTCTC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 384
Eco57I CTGAAG 2 cut(s) 101, 138
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 579
EcoRI GAATTC 1 cut(s) 538
EcoT38I GRGCYC 2 cut(s) 50, 386
FaeI CATG 3 cut(s) 317, 358, 448
FaiI YATR 9 cut(s) 136, 315, 356, 390, 392, 400, 446, 533, 593
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FatI CATG 3 cut(s) 313, 354, 444
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 5 cut(s) 35, 126, 265, 334, 644
FokI GGATG 2 cut(s) 337, 617
FriOI GRGCYC 2 cut(s) 50, 386
Fsp4HI GCNGC 5 cut(s) 35, 126, 265, 334, 644
FspBI CTAG 2 cut(s) 98, 258
GlaI GCGC 1 cut(s) 38
GluI GCNGC 5 cut(s) 35, 126, 265, 334, 644
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 1 cut(s) 580
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 3 cut(s) 317, 358, 448
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 1 cut(s) 419
HindII GTYRAC 1 cut(s) 419
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 1 cut(s) 275
HphI GGTGA 2 cut(s) 16, 239
Hpy166II GTNNAC 1 cut(s) 419
Hpy188III TCNNGA 2 cut(s) 279, 445
Hpy8I GTNNAC 1 cut(s) 419
HpyAV CCTTC 2 cut(s) 81, 286
HpyCH4III ACNGT 3 cut(s) 232, 451, 484
HpyCH4IV ACGT 2 cut(s) 104, 409
HpyCH4V TGCA 4 cut(s) 313, 413, 628, 662
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 122, 131, 319
HpyF3I CTNAG 3 cut(s) 58, 207, 323
HpySE526I ACGT 2 cut(s) 104, 409
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 3 cut(s) 317, 358, 448
HspAI GCGC 1 cut(s) 37
KpnI GGTACC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 5 cut(s) 236, 244, 281, 505, 544
LguI GCTCTTC 1 cut(s) 633
LpnPI CCDG 4 cut(s) 16, 28, 373, 399
Lsp1109I GCAGC 3 cut(s) 46, 137, 630
MaeI CTAG 2 cut(s) 98, 258
MaeII ACGT 2 cut(s) 104, 409
MalI GATC 5 cut(s) 238, 246, 283, 507, 546
MbiI CCGCTC 1 cut(s) 162
MboI GATC 5 cut(s) 236, 244, 281, 505, 544
MboII GAAGA 7 cut(s) 94, 131, 478, 481, 620, 623, 650
MfeI CAATTG 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 50, 386
MluCI AATT 7 cut(s) 181, 436, 527, 538, 618, 655, 705
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 4 cut(s) 17, 360, 570, 691
MseI TTAA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 116
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 684
MwoI GCNNNNNNNGC 4 cut(s) 45, 122, 131, 319
NdeII GATC 5 cut(s) 236, 244, 281, 505, 544
NlaIII CATG 3 cut(s) 317, 358, 448
NlaIV GGNNCC 2 cut(s) 220, 581
NspI RCATGY 1 cut(s) 317
PaeI GCATGC 1 cut(s) 317
PagI TCATGA 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 633
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 592
PkrI GCNGC 5 cut(s) 36, 127, 266, 335, 645
Psp124BI GAGCTC 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 220, 581
PspPI GGNCC 1 cut(s) 579
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 4 cut(s) 20, 220, 226, 481
RsaNI GTAC 4 cut(s) 19, 219, 225, 480
SacI GAGCTC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 5 cut(s) 35, 126, 265, 334, 644
Sau3AI GATC 5 cut(s) 236, 244, 281, 505, 544
Sau96I GGNCC 1 cut(s) 579
ScaI AGTACT 1 cut(s) 481
SduI GDGCHC 2 cut(s) 50, 386
SetI ASST 9 cut(s) 107, 118, 148, 324, 362, 386, 412, 418, 645
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
SpeI ACTAGT 1 cut(s) 257
SphI GCATGC 1 cut(s) 317
Sse9I AATT 7 cut(s) 181, 436, 527, 538, 618, 655, 705
SsiI CCGC 5 cut(s) 67, 162, 264, 333, 596
SspMI CTAG 2 cut(s) 98, 258
SstI GAGCTC 1 cut(s) 386
TaaI ACNGT 3 cut(s) 232, 451, 484
TaiI ACGT 2 cut(s) 107, 412
TaqI TCGA 4 cut(s) 11, 372, 536, 557
TasI AATT 7 cut(s) 181, 436, 527, 538, 618, 655, 705
TatI WGTACW 2 cut(s) 18, 479
TauI GCSGC 2 cut(s) 267, 336
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 1 cut(s) 47
TseI GCWGC 3 cut(s) 34, 125, 643
TspDTI ATGAA 2 cut(s) 156, 621
TspGWI ACGGA 2 cut(s) 139, 666
TspRI CASTG 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 592
XagI CCTNNNNNAGG 1 cut(s) 364
XapI RAATTY 2 cut(s) 538, 705
XceI RCATGY 1 cut(s) 317
XspI CTAG 2 cut(s) 98, 258
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.