Rorug01G0482900

Ethylene-responsive transcription factor WIN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003109
Physical Location & Seq
Reverse (-)
57030570 .. 57033335
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0482900.1

Sequence Viewer

Length: 582 bp
ATGGGTCAAGCTTTTCGGAAGCTCTTCGATGCCTTCTTCGGAAACACTGAGATGCGAGTTGTGATGCTTGGACTAGATGCGGCGGGTAAAACAACCATCCTTTACAAACTGCATATTGGAGAAGTCTTGTCAACTGTTCCTACAATAGGTTTTAATGTGGAAAAAGTTCAGTACAAGAATGTGATGTTCACAGTTTGGGATGTTGGTGGGCAAGAGAAACTAAGGCCGCTCTGGAGGCATTACTTTAATAATACGGATGGACTGATTTATGTCGTTGACTCTTTGGACAGGGAGAGAATTGGACGAGCCAAGGCAGAATTTCAGGCCATAATCAGTGATCCATTTATGCTCAACAGTGTCATCTTGGTGTTTGCGAACAAACAGGATATGAAAGGAGCTATGTCCCCAATGGAAGTATGTGAAGGGCTAGGCCTGTTTGATCTCAAGAACAGAAAATGGCACATACAGGGGACCTGTGCCCTTCGGGGTGATGGCCTTTATGAGGGCTTGGATTGGCTAGCTAACACCCTCAAGGAGATGAGAGCTGCTGGATACTCTTCAGTGGGCACCTCATCATTCTAA

Protein Analysis

193

Amino Acids

21.72

Weight (kDa)

7.75

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 6 - 176 1.4e-70 ADP-ribosylation factor family
SRPRB PF09439 17 - 139 6.8e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 2.9e-12 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 132 3.4e-10 Ras family
Gtr1_RagA PF04670 20 - 129 3.7e-08 Gtr1/RagA G protein conserved region
G-alpha PF00503 44 - 130 4.2e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 229
AciI CCGC 3 cut(s) 80, 83, 227
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 146, 502
AluBI AGCT 5 cut(s) 11, 22, 398, 521, 545
AluI AGCT 5 cut(s) 11, 22, 398, 521, 545
AlwI GGATC 1 cut(s) 332
AoxI GGCC 4 cut(s) 224, 324, 430, 493
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 317
Asp700I GAANNNNTTC 2 cut(s) 23, 165
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 1 cut(s) 500
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 2 cut(s) 481, 569
BanI GGYRCC 1 cut(s) 566
BbvI GCAGC 1 cut(s) 532
BccI CCATC 3 cut(s) 104, 251, 485
BciVI GTATCC 1 cut(s) 545
BfaI CTAG 3 cut(s) 74, 428, 518
BfuI GTATCC 1 cut(s) 545
BisI GCNGC 3 cut(s) 81, 227, 546
BlsI GCNGC 3 cut(s) 82, 228, 547
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 2 cut(s) 472, 568
BmsI GCATC 4 cut(s) 19, 42, 54, 67
BmtI GCTAGC 1 cut(s) 521
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 428, 515
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 502
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 3 cut(s) 96, 205, 262
BseLI CCNNNNNNNGG 2 cut(s) 146, 502
BseMII CTCAG 1 cut(s) 39
BseSI GKGCMC 2 cut(s) 481, 569
BseXI GCAGC 1 cut(s) 532
BshFI GGCC 4 cut(s) 226, 326, 432, 495
BshNI GGYRCC 1 cut(s) 566
BslFI GGGAC 2 cut(s) 388, 484
BslI CCNNNNNNNGG 2 cut(s) 146, 502
BsmFI GGGAC 2 cut(s) 388, 484
BsnI GGCC 4 cut(s) 226, 326, 432, 495
Bsp1286I GDGCHC 2 cut(s) 481, 569
Bsp143I GATC 2 cut(s) 337, 439
BspACI CCGC 3 cut(s) 80, 83, 227
BspANI GGCC 4 cut(s) 226, 326, 432, 495
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 472, 568
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 566
BsrBI CCGCTC 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 2 cut(s) 337, 439
BssT1I CCWWGG 1 cut(s) 309
Bst4CI ACNGT 3 cut(s) 136, 193, 356
Bst6I CTCTTC 2 cut(s) 29, 562
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 2 cut(s) 48, 221
BstENI CCTNNNNNAGG 2 cut(s) 144, 500
BstF5I GGATG 3 cut(s) 96, 205, 262
BstKTI GATC 2 cut(s) 340, 442
BstMBI GATC 2 cut(s) 337, 439
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstSLI GKGCMC 2 cut(s) 481, 569
BstV1I GCAGC 1 cut(s) 532
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 226, 326, 432, 495
BtsCI GGATG 3 cut(s) 96, 205, 262
BtsIMutI CAGTG 4 cut(s) 45, 340, 361, 567
Cac8I GCNNGC 1 cut(s) 519
Cfr13I GGNCC 1 cut(s) 471
Csp6I GTAC 1 cut(s) 172
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 2 cut(s) 48, 221
DpnI GATC 2 cut(s) 339, 441
DpnII GATC 2 cut(s) 337, 439
Eam1104I CTCTTC 2 cut(s) 29, 562
EarI CTCTTC 2 cut(s) 29, 562
Eco130I CCWWGG 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 1 cut(s) 543
EcoNI CCTNNNNNAGG 2 cut(s) 144, 500
EcoO109I RGGNCCY 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 9 cut(s) 114, 270, 329, 347, 389, 401, 418, 464, 501
FaqI GGGAC 2 cut(s) 388, 484
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 3 cut(s) 81, 227, 546
FokI GGATG 3 cut(s) 83, 212, 269
Fsp4HI GCNGC 3 cut(s) 81, 227, 546
FspBI CTAG 3 cut(s) 74, 428, 518
GluI GCNGC 3 cut(s) 81, 227, 546
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 4 cut(s) 226, 326, 432, 495
HincII GTYRAC 2 cut(s) 132, 277
HindII GTYRAC 2 cut(s) 132, 277
HindIII AAGCTT 1 cut(s) 9
HinfI GANTC 1 cut(s) 278
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 3 cut(s) 132, 189, 277
Hpy188I TCNGA 2 cut(s) 18, 41
Hpy188III TCNNGA 2 cut(s) 232, 445
Hpy8I GTNNAC 3 cut(s) 132, 189, 277
HpyAV CCTTC 3 cut(s) 43, 416, 491
HpyCH4III ACNGT 3 cut(s) 136, 193, 356
HpyCH4V TGCA 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 2 cut(s) 48, 221
Kzo9I GATC 2 cut(s) 337, 439
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 1 cut(s) 395
LpnPI CCDG 8 cut(s) 217, 274, 308, 368, 446, 452, 487, 534
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 4 cut(s) 19, 42, 54, 67
MaeI CTAG 3 cut(s) 74, 428, 518
MalI GATC 2 cut(s) 339, 441
MbiI CCGCTC 1 cut(s) 229
MboI GATC 2 cut(s) 337, 439
MboII GAAGA 3 cut(s) 16, 28, 549
MhlI GDGCHC 2 cut(s) 481, 569
MluCI AATT 2 cut(s) 297, 317
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 4 cut(s) 228, 496, 539, 580
MroXI GAANNNNTTC 2 cut(s) 23, 165
MseI TTAA 2 cut(s) 153, 246
MslI CAYNNNNRTG 2 cut(s) 50, 365
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 2 cut(s) 337, 439
NheI GCTAGC 1 cut(s) 517
NlaIV GGNNCC 2 cut(s) 472, 568
PceI AGGCCT 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 29
PdmI GAANNNNTTC 2 cut(s) 23, 165
PkrI GCNGC 3 cut(s) 82, 228, 547
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 471
PspN4I GGNNCC 2 cut(s) 472, 568
PspPI GGNCC 1 cut(s) 471
PspPPI RGGWCCY 1 cut(s) 471
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 50, 365
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 153, 246
SatI GCNGC 3 cut(s) 81, 227, 546
Sau3AI GATC 2 cut(s) 337, 439
Sau96I GGNCC 1 cut(s) 471
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 2 cut(s) 481, 569
SetI ASST 8 cut(s) 13, 24, 151, 400, 476, 523, 547, 572
SfaNI GCATC 4 cut(s) 19, 42, 54, 67
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 2 cut(s) 50, 365
SmlI CTYRAG 2 cut(s) 443, 530
SmoI CTYRAG 2 cut(s) 443, 530
Sse9I AATT 2 cut(s) 297, 317
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 3 cut(s) 80, 83, 227
SspMI CTAG 3 cut(s) 74, 428, 518
StuI AGGCCT 1 cut(s) 432
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 136, 193, 356
TaqI TCGA 1 cut(s) 27
TasI AATT 2 cut(s) 297, 317
TatI WGTACW 1 cut(s) 171
TauI GCSGC 2 cut(s) 83, 229
Tru1I TTAA 2 cut(s) 153, 246
Tru9I TTAA 2 cut(s) 153, 246
TscAI CASTG 4 cut(s) 52, 340, 361, 567
TseI GCWGC 1 cut(s) 545
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 4 cut(s) 52, 340, 361, 567
VpaK11BI GGWCC 1 cut(s) 471
XagI CCTNNNNNAGG 2 cut(s) 144, 500
XapI RAATTY 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 23, 165
XspI CTAG 3 cut(s) 74, 428, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.