Rw2G003000

Ethylene-responsive transcription factor WIN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003109
Physical Location & Seq
Reverse (-)
2832357 .. 2833696
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003000.1

Sequence Viewer

Length: 675 bp
ATGTGCATGCTTAGAATCCATCTCCTAGCTCTCTATCTCTTTTTCAAATTGGCAAAAATGGTGCAGTCAACAAAGAAGTTTAGAGGTGTCCGGCAGCGGAATTGGGGTTCTTGGGTCTCTGAAATTCGTCATCCACTGCTGAAGAGAAGGGTGTGGCTAGGCACATTTGACAGTGCTGAAGAGGCGGCAAGAGCATATGATGCAGCTGCCATTTTGATGAGTGGCCGAAATGCCAAAACAAATTTCCCAACAACTACTCAAACTAGTAGTAGTACTACTCCGAAAACTAGTACTATTAAGGATTCTTCGTCAACACCAAATGATTTATCAGATATCTTACGCGCAAAGCTGAGGAAATGTAGCAACGTACTCTCTCCATCCCTGACGTGCTTGAGGCTGGACAACGAGAGCTCTCATATCGGAGTCTGGCAAAAGCGAGCAGGTCAGCGATCTGATCATTCCAACTGGGTCATGACTGTCCAACTTGGAAAGAAGAATATTAAAGCTGATGATGGTACCACCAACCAGCAAAGTGTTTCATCACCATCAATGTTTTCAGAGTCTCCAGCATCACCATCATTAGAAAAGTCTAAACTAATAGCAGATGCTTATGAAATTACTGAAGAGGAGAGAATTTCATTGCAGATGATAGAGGAGCTGCTTAACAGAAATTGA

Protein Analysis

224

Amino Acids

25.2

Weight (kDa)

9.71

Isoelectric Point (pI)

67.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 26 - 73 7.3e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 431
Acc65I GGTACC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 515
AccII CGCG 1 cut(s) 342
AciI CCGC 2 cut(s) 97, 185
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 3 cut(s) 123, 241, 633
AcuI CTGAAG 3 cut(s) 161, 198, 642
AfaI GTAC 4 cut(s) 274, 292, 369, 517
AgsI TTSAA 1 cut(s) 46
AhlI ACTAGT 2 cut(s) 263, 287
AjiI CACGTC 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AloI GAACNNNNNNTCC 2 cut(s) 91, 123
AluBI AGCT 6 cut(s) 29, 206, 349, 411, 506, 658
AluI AGCT 6 cut(s) 29, 206, 349, 411, 506, 658
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 2 cut(s) 121, 567
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 4 cut(s) 94, 203, 206, 658
ApoI RAATTY 3 cut(s) 123, 241, 633
Asp718I GGTACC 1 cut(s) 515
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 534, 564
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 413
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 4 cut(s) 106, 193, 215, 645
BccI CCATC 5 cut(s) 27, 385, 506, 553, 583
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 121, 567
BcuI ACTAGT 2 cut(s) 263, 287
BfaI CTAG 4 cut(s) 26, 158, 264, 288
BfuAI ACCTGC 1 cut(s) 431
BisI GCNGC 5 cut(s) 95, 186, 204, 207, 659
BlsI GCNGC 5 cut(s) 96, 187, 205, 208, 660
BmcAI AGTACT 2 cut(s) 274, 292
BmgBI CACGTC 1 cut(s) 387
BmiI GGNNCC 1 cut(s) 517
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 3 cut(s) 190, 578, 595
BmuI ACTGGG 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 470
Bse3DI GCAATG 1 cut(s) 638
BseGI GGATG 2 cut(s) 130, 377
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 2 cut(s) 641, 668
BseXI GCAGC 4 cut(s) 106, 193, 215, 645
BsgI GTGCAG 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 225
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 91
BsmAI GTCTC 2 cut(s) 121, 567
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 413
Bsp143I GATC 2 cut(s) 449, 454
BspACI CCGC 2 cut(s) 97, 185
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 342
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 517
BspMI ACCTGC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 1 cut(s) 470
BssMI GATC 2 cut(s) 449, 454
Bst4CI ACNGT 2 cut(s) 173, 478
Bst6I CTCTTC 3 cut(s) 137, 174, 618
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 8, 438
BstDEI CTNAG 2 cut(s) 11, 350
BstF5I GGATG 2 cut(s) 130, 377
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 2 cut(s) 452, 457
BstMAI GTCTC 2 cut(s) 121, 567
BstMBI GATC 2 cut(s) 449, 454
BstMWI GCNNNNNNNGC 3 cut(s) 182, 191, 200
BstNSI RCATGY 1 cut(s) 10
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 4 cut(s) 106, 193, 215, 645
BsuRI GGCC 1 cut(s) 225
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 2 cut(s) 130, 377
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 134, 178
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 8, 438
CciI TCATGA 1 cut(s) 471
CfoI GCGC 1 cut(s) 344
Csp6I GTAC 4 cut(s) 273, 291, 368, 516
CviAII CATG 2 cut(s) 7, 472
CviJI RGCY 9 cut(s) 29, 157, 206, 225, 349, 397, 411, 506, 658
CviKI_1 RGCY 9 cut(s) 29, 157, 206, 225, 349, 397, 411, 506, 658
CviQI GTAC 4 cut(s) 273, 291, 368, 516
DdeI CTNAG 2 cut(s) 11, 350
DpnI GATC 2 cut(s) 451, 456
DpnII GATC 2 cut(s) 449, 454
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 3 cut(s) 137, 174, 618
EarI CTCTTC 3 cut(s) 137, 174, 618
Ecl136II GAGCTC 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 121
Eco32I GATATC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 411
Eco57I CTGAAG 3 cut(s) 161, 198, 642
EcoICRI GAGCTC 1 cut(s) 411
EcoRV GATATC 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 2 cut(s) 10, 475
FaiI YATR 6 cut(s) 8, 196, 198, 417, 473, 612
FatI CATG 2 cut(s) 6, 471
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 5 cut(s) 95, 186, 204, 207, 659
FokI GGATG 2 cut(s) 117, 364
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 5 cut(s) 95, 186, 204, 207, 659
FspBI CTAG 4 cut(s) 26, 158, 264, 288
GlaI GCGC 1 cut(s) 343
GluI GCNGC 5 cut(s) 95, 186, 204, 207, 659
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 91
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 10, 475
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 2 cut(s) 69, 312
HindII GTYRAC 2 cut(s) 69, 312
HinfI GANTC 4 cut(s) 15, 302, 423, 560
HpaII CCGG 1 cut(s) 91
HphI GGTGA 2 cut(s) 534, 564
Hpy166II GTNNAC 2 cut(s) 69, 312
Hpy188I TCNGA 6 cut(s) 121, 282, 331, 422, 454, 559
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 2 cut(s) 69, 312
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 2 cut(s) 173, 478
HpyCH4IV ACGT 2 cut(s) 366, 386
HpyCH4V TGCA 4 cut(s) 6, 64, 203, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 191, 200
HpyF3I CTNAG 2 cut(s) 11, 350
HpySE526I ACGT 2 cut(s) 366, 386
Hsp92II CATG 2 cut(s) 10, 475
HspAI GCGC 1 cut(s) 342
KpnI GGTACC 1 cut(s) 519
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 2 cut(s) 449, 454
LmnI GCTCC 1 cut(s) 655
LpnPI CCDG 8 cut(s) 104, 383, 395, 412, 426, 451, 539, 579
Lsp1109I GCAGC 4 cut(s) 106, 193, 215, 645
LweI GCATC 3 cut(s) 190, 578, 595
MaeI CTAG 4 cut(s) 26, 158, 264, 288
MaeII ACGT 2 cut(s) 366, 386
MalI GATC 2 cut(s) 451, 456
MboI GATC 2 cut(s) 449, 454
MboII GAAGA 5 cut(s) 154, 191, 297, 505, 635
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 7 cut(s) 47, 100, 123, 241, 615, 633, 670
MlyI GAGTC 2 cut(s) 432, 569
MmeI TCCRAC 2 cut(s) 486, 505
MnlI CCTC 6 cut(s) 77, 175, 345, 387, 619, 646
MseI TTAA 3 cut(s) 297, 501, 663
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 2 cut(s) 97, 206
MspI CCGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 182, 191, 200
NdeI CATATG 1 cut(s) 196
NdeII GATC 2 cut(s) 449, 454
NlaIII CATG 2 cut(s) 10, 475
NlaIV GGNNCC 1 cut(s) 517
NspI RCATGY 1 cut(s) 10
PaeI GCATGC 1 cut(s) 10
PagI TCATGA 1 cut(s) 471
PfeI GAWTC 2 cut(s) 15, 302
PkrI GCNGC 5 cut(s) 96, 187, 205, 208, 660
PleI GAGTC 2 cut(s) 431, 568
PpsI GAGTC 2 cut(s) 431, 568
Psp124BI GAGCTC 1 cut(s) 413
PspN4I GGNNCC 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 4 cut(s) 274, 292, 369, 517
RsaNI GTAC 4 cut(s) 273, 291, 368, 516
RseI CAYNNNNRTG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 413
SaqAI TTAA 3 cut(s) 297, 501, 663
SatI GCNGC 5 cut(s) 95, 186, 204, 207, 659
Sau3AI GATC 2 cut(s) 449, 454
ScaI AGTACT 2 cut(s) 274, 292
SchI GAGTC 2 cut(s) 432, 569
SduI GDGCHC 1 cut(s) 413
SfaNI GCATC 3 cut(s) 190, 578, 595
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SpeI ACTAGT 2 cut(s) 263, 287
SphI GCATGC 1 cut(s) 10
Sse9I AATT 7 cut(s) 47, 100, 123, 241, 615, 633, 670
SsiI CCGC 2 cut(s) 97, 185
SspI AATATT 1 cut(s) 499
SspMI CTAG 4 cut(s) 26, 158, 264, 288
SstI GAGCTC 1 cut(s) 413
TaaI ACNGT 2 cut(s) 173, 478
TaiI ACGT 2 cut(s) 369, 389
TasI AATT 7 cut(s) 47, 100, 123, 241, 615, 633, 670
TatI WGTACW 2 cut(s) 272, 290
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 15, 302
Tru1I TTAA 3 cut(s) 297, 501, 663
Tru9I TTAA 3 cut(s) 297, 501, 663
TscAI CASTG 2 cut(s) 141, 178
TseI GCWGC 4 cut(s) 94, 203, 206, 658
TspDTI ATGAA 3 cut(s) 528, 627, 627
TspRI CASTG 2 cut(s) 141, 178
XapI RAATTY 3 cut(s) 123, 241, 633
XceI RCATGY 1 cut(s) 10
XspI CTAG 4 cut(s) 26, 158, 264, 288
ZrmI AGTACT 2 cut(s) 274, 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.