Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

3649476 .. 3654066

Gene Structure 4591 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1045500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGACCGCTCCTCCTCCTCGCGCCGCCACGTCATCACTCTCTATCCTCTTCGTCGTGATCTCCATTTCAGTTTCCATCTCCATCTCTCTCCTCCATTTCAGACCCCATAATTCCCCAACCCTAAGCCGAAACCTTCCCCCAAATTCGTGGCGTCCCCAGGCCAACCGCCAAAGGTACGTCGTTCGATTTGTGGAGTACAGGAGGGCGGAGGAGCTCCGAGAGTATTTGAAATCGGGTGTGCGATCCGATGGGTGGGATTGGATCGAGAGGAACAACCCGGCGAAGAAGTATCCGACGGATTTCGGGTTGGTTTGGATTGAAGACTCGGCGAAAGAAAGGGTATTTTCGGAAATTAGGAGCTTGGGAATGGTGAAGGATGTGAATGCGGATGTGAGGTATGGAAGGGGTTTGCTTGCTGAGGGGAAAAGGGACAGAGTTGGGGCTTTTGTCGATGGGAAGAAGCGGCCGGGGAAGATTTTGACGGGGATGTCGTTCAGTGAAGCCGGCGGCGATGCGTTTTACTCCGCCACAAGCAATTCTTCGATTCGCTGGAGAAGGCAGCTCATGTCTCAGAGATCTCAAGTTACTTCATTGTTTGGGGCTGAGTCCCTTTGGGAAAAAGGTTATACTGGTGCAAATGTCAAAATGGCCATCTTTGACACTGGAATTCGAGCTAATCACCCGCATTTTCGTAATATCAAGGAGCGCACAAACTGGACAAATGAAGATACTTTAAATGACAATCTTGGGCATGGGACATTTGTTGCTGGTGTTATTGCTGGAGTAGATGCAGAGTGTCTTGGTTATGCTCCTGACACAGAGATTTATGCTTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGACGCTTTCAACTATGCAATTGCAACCAATATGGATGTGCTAAATCTGAGTATAGGTGGACCTGATTACTTGGACCTCCCATTTGTAGAGAAGGTCTGGGAAATAACTGCAAACAACATAATTATGGTTTCGGCAATTGGAAATGACGGACCACTTTATGGAACTCTAAACAATCCAGCAGACCAAAGTGATATCATTGGTGTTGGTGGCATTGACTACAGTGACCACATTGCTTCTTTCTCGTCACGTGGCATGAGTACATGGGAGATGCCTCATGGTTATGGTCGGGTGAAGCCAGATGTTGTTGCATATGGACGGGACATCATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCAGGCACAAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTATGTCTTCTTGTCAGTGTTATCCCTGTGAGCAATAGGAAGGATATTTTAAATCCAGCAAGCATGAAACAAGCACTAGTTCAGGGTGCTGCGAAGCTTTCTGGTCCGAATATGTTTGAGCAGGGTGCAGGCAGGGTTGATCTGTTAGAATCATATGAAATCCTCAAGAGTTACCAACCTCGTGCCAGCATCTTCCCTAGCACTCTTGATTATACGGATTGTCCTTACTCTTGGCCCTTCTGTCGTCAGCCCCTTTATGCAGGTGCCATGCCTGTTATCTTTAATGCCACCATTCTAAATGGGATGGGTGTAATAGGCTACGTTGAAAGTCCACCGACATGGCATCCTTCAGATGATGTAGGGAATCTTCTAAGCATTCACTTTACTTATTCTGAAGTTATTTGGCCTTGGACTGGTTATTTAGCACTTCACATGCAAATTAAGGAAGAAGGGGCACAATTTTCTGGAGAGATAGGAGGCAATGTTACTCTTAGGGTATACAGTCCTCCAGCTCTAGGAGAGACGGATCGTCAGATGAGTACTTGTGTGCTTCAGTTGAAATTGAAAATTGTCCCAACTCCACCAAGATCGAAACGAGTTTTGTGGGATCAGTTTCACAATATTAAATACCCTCCAGGTTATATCCCTAGAGATTCTTTGGATGTCCGTAATGACATTCTTGATTGGCATGGGGATCACCTGCATACGAATTTTCACATTATGTTCAACATGTTACGAGACGCTGGGTATTATGTCGAAACACTCGGTTCGCCATTTACTTGCTTTGATGCTCTCCGGTATGGGACCCTTCTTCTTGTAGATCTTGAAGAAGAGTACTTTCAGGATGAGATTGAAAAGCTTCGAGACGATGTTCTTAATTCTGGACTGGGATTAGTGGTGTTTTCTGAATGGTATAATGTGGACACAATGGTGAAGATGAGATTCTTTGATGATAACACACGGAGCTGGTGGACTCCAGTCACTGGTGGCGCAAATGTTCCTGCGTTGAATGACCTTTTAGCTCCATTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATTAATGGTGAGCATAGTCGATATGCATCTGGAACGGATATTGTGAGGTTTCCACGAGGTGGTTATGTGCACAAGTTCCCTTTCGTTGATAGCTCTGAGAGTGGGGCTACTCAAAATGTACTTCGGACTCCTGAGATGACCACGGCAGATTCTCCCATTCTTGGTCTTGTGGAGGCGGGTGAAGGTCGTGTTGTAGTCTATGGAGATTCTAACTGTTTGGACAGCAGTCACATGGTGACGAATTGTTATTGGCTCTTGAGGAAAATATTAGATTTCACCGGTGGAAACATCAAAGATCCCGTGCTTTTCTCAAACTCGGTTAAACAAAGTTCACCTTTACATATAGAAGACAACCAATTACCATCTCGTAGGACTGACGTGAATTTTTCTACATATTCTGCTGTTATGGGAAGGGAATTGATGTGTGGAAGTGACTCCGTATTTGAAATCTGGGGGACTAAAGGTTACAGTTCACAGGTCAGGGGAACAAACAGAAGATTACCGGGCTATTCTGTGGTTGACTTGGATAGAGGTTTAAATTCTACAGCCACAACAACAACAACAACAAAGCCTTTTCCCACTAAGTGGGGTCGGCTATATGAATCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

986

Amino Acids

109.6

Weight (kDa)

5.88

Isoelectric Point (pI)

42.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	57 - 129	1.5e-13	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	211 - 467	2.2e-41	Subtilase family
MBTPS1_3rd	PF23094	491 - 619	1e-53	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	620 - 891	1.2e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1529, 1986
AasI	GACNNNNNNGTC	1 cut(s)	487
Acc36I	ACCTGC	2 cut(s)	1529, 1986
AccB1I	GGYRCC	1 cut(s)	1541
AccB7I	CCANNNNNTGG	4 cut(s)	1037, 2261, 2408, 2934
AccBSI	CCGCTC	1 cut(s)	8
AccI	GTMKAC	1 cut(s)	1776
AccII	CGCG	1 cut(s)	21
AclWI	GGATC	8 cut(s)	237, 269, 1203, 1216, 1812, 1893, 1980, 2639
AcoI	YGGCCR	2 cut(s)	464, 648
AcsI	RAATTY	5 cut(s)	142, 666, 1987, 2731, 2887
AcuI	CTGAAG	3 cut(s)	1611, 1692, 1814
AcvI	CACGTG	1 cut(s)	1127
AcyI	GRCGYC	1 cut(s)	151
AdeI	CACNNNGTG	3 cut(s)	2408, 2584, 2934
AfaI	GTAC	7 cut(s)	176, 197, 1138, 1222, 1819, 2114, 2469
AfiI	CCNNNNNNNGG	8 cut(s)	252, 1037, 1691, 1793, 2261, 2408, 2510, 2934
AflIII	ACRYGT	1 cut(s)	2007
AgeI	ACCGGT	1 cut(s)	2627
AhdI	GACNNNNNGTC	1 cut(s)	1806
AhlI	ACTAGT	1 cut(s)	1354
AjiI	CACGTC	2 cut(s)	30, 2728
AjnI	CCWGG	2 cut(s)	156, 1912
AleI	CACNNNNGTG	2 cut(s)	1250, 2207
Alw21I	GWGCWC	2 cut(s)	216, 2421
Alw26I	GTCTC	4 cut(s)	573, 1793, 2010, 2136
Alw44I	GTGCAC	1 cut(s)	2417
AlwI	GGATC	8 cut(s)	237, 269, 1203, 1216, 1812, 1893, 1980, 2639
AlwNI	CAGNNNCTG	1 cut(s)	2261
AoxI	GGCC	5 cut(s)	159, 464, 648, 1511, 1682
ApaLI	GTGCAC	1 cut(s)	2417
ApeKI	GCWGC	2 cut(s)	559, 1367
ApoI	RAATTY	5 cut(s)	142, 666, 1987, 2731, 2887
ArsI	GACNNNNNNTTYG	4 cut(s)	944, 976, 2774, 2806
AseI	ATTAAT	1 cut(s)	2352
AsiGI	ACCGGT	1 cut(s)	2627
Asp700I	GAANNNNTTC	1 cut(s)	2136
AspLEI	GCGC	3 cut(s)	23, 708, 2270
AspS9I	GGNCC	6 cut(s)	938, 952, 1028, 1382, 1512, 2082
AsuC2I	CCSGG	3 cut(s)	278, 468, 2853
AvaII	GGWCC	5 cut(s)	938, 952, 1028, 1382, 2082
BaeGI	GKGCMC	2 cut(s)	1735, 2421
BalI	TGGCCA	1 cut(s)	650
BamHI	GGATCC	1 cut(s)	1208
BanI	GGYRCC	1 cut(s)	1541
BanII	GRGCYC	1 cut(s)	216
BarI	GAAGNNNNNNTAC	2 cut(s)	1629, 1661
BauI	CACGAG	2 cut(s)	1458, 2403
BbrPI	CACGTG	1 cut(s)	1127
BbsI	GAAGAC	3 cut(s)	327, 1277, 2703
Bbv12I	GWGCWC	2 cut(s)	216, 2421
BbvCI	CCTCAGC	1 cut(s)	417
BbvI	GCAGC	2 cut(s)	571, 1354
BccI	CCATC	7 cut(s)	83, 89, 242, 446, 659, 1576, 2719
BceAI	ACGGC	1 cut(s)	2508
BciT130I	CCWGG	2 cut(s)	158, 1914
BciVI	GTATCC	1 cut(s)	300
BcnI	CCSGG	3 cut(s)	278, 468, 2853
BcoDI	GTCTC	4 cut(s)	573, 1793, 2010, 2136
BcuI	ACTAGT	1 cut(s)	1354
BfaI	CTAG	5 cut(s)	1355, 1476, 1793, 1926, 2956
BfmI	CTRYAG	2 cut(s)	1096, 2892
BfuAI	ACCTGC	2 cut(s)	1529, 1986
BfuI	GTATCC	1 cut(s)	300
BglII	AGATCT	2 cut(s)	575, 2098
BisI	GCNGC	5 cut(s)	24, 464, 508, 560, 1368
BlsI	GCNGC	5 cut(s)	25, 465, 509, 561, 1369
BmcAI	AGTACT	2 cut(s)	1819, 2114
Bme1390I	CCNGG	5 cut(s)	158, 278, 468, 1914, 2853
Bme18I	GGWCC	5 cut(s)	938, 952, 1028, 1382, 2082
BmeRI	GACNNNNNGTC	1 cut(s)	1806
BmgBI	CACGTC	2 cut(s)	30, 2728
BmgT120I	GGNCC	6 cut(s)	938, 952, 1028, 1382, 1512, 2082
BmiI	GGNNCC	5 cut(s)	875, 1210, 1543, 2083, 2084
BmrFI	CCNGG	5 cut(s)	158, 278, 468, 1914, 2853
BmrI	ACTGGG	1 cut(s)	2174
BmsI	GCATC	8 cut(s)	502, 778, 838, 1137, 1476, 1630, 2056, 2384
BmuI	ACTGGG	1 cut(s)	2174
BoxI	GACNNNNGTC	2 cut(s)	2255, 2574
BpiI	GAAGAC	3 cut(s)	327, 1277, 2703
BpmI	CTGGAG	6 cut(s)	571, 801, 1764, 1770, 1896, 2238
Bpu10I	CCTNAGC	2 cut(s)	122, 417
BpuEI	CTTGAG	3 cut(s)	564, 1427, 2626
BpuMI	CCSGG	3 cut(s)	278, 468, 2853
BsaAI	YACGTR	1 cut(s)	1127
BsaBI	GATNNNNATC	1 cut(s)	2374
BsaHI	GRCGYC	1 cut(s)	151
BsaJI	CCNNGG	4 cut(s)	156, 467, 1685, 2490
BsaWI	WCCGGW	2 cut(s)	2073, 2627
BsaXI	ACNNNNNCTCC	3 cut(s)	25, 1746, 1776
Bsc4I	CCNNNNNNNGG	8 cut(s)	252, 1037, 1691, 1793, 2261, 2408, 2510, 2934
Bse118I	RCCGGY	2 cut(s)	503, 2627
Bse1I	ACTGG	7 cut(s)	634, 667, 719, 1696, 2169, 2255, 2266
Bse3DI	GCAATG	2 cut(s)	1107, 1765
Bse8I	GATNNNNATC	1 cut(s)	2374
BseBI	CCWGG	2 cut(s)	158, 1914
BseDI	CCNNGG	4 cut(s)	156, 467, 1685, 2490
BseGI	GGATG	8 cut(s)	382, 394, 492, 919, 1587, 1621, 1945, 2128
BseJI	GATNNNNATC	1 cut(s)	2374
BseLI	CCNNNNNNNGG	8 cut(s)	252, 1037, 1691, 1793, 2261, 2408, 2510, 2934
BseMI	GCAATG	2 cut(s)	1107, 1765
BseMII	CTCAG	6 cut(s)	408, 584, 594, 917, 2436, 2472
BseNI	ACTGG	7 cut(s)	634, 667, 719, 1696, 2169, 2255, 2266
BseRI	GAGGAG	3 cut(s)	6, 80, 224
BseSI	GKGCMC	2 cut(s)	1735, 2421
BseX3I	CGGCCG	1 cut(s)	464
BseXI	GCAGC	2 cut(s)	571, 1354
BseYI	CCCAGC	1 cut(s)	2021
BsgI	GTGCAG	1 cut(s)	1425
Bsh1236I	CGCG	1 cut(s)	21
Bsh1285I	CGRYCG	1 cut(s)	467
BshFI	GGCC	5 cut(s)	161, 466, 650, 1513, 1684
BshNI	GGYRCC	1 cut(s)	1541
BshTI	ACCGGT	1 cut(s)	2627
BsiEI	CGRYCG	1 cut(s)	467
BsiHKAI	GWGCWC	2 cut(s)	216, 2421
BsiSI	CCGG	6 cut(s)	278, 467, 504, 2074, 2628, 2852
BslFI	GGGAC	8 cut(s)	138, 443, 592, 769, 1211, 1835, 2095, 2818
BslI	CCNNNNNNNGG	8 cut(s)	252, 1037, 1691, 1793, 2261, 2408, 2510, 2934
BsmAI	GTCTC	4 cut(s)	573, 1793, 2010, 2136
BsmBI	CGTCTC	3 cut(s)	1793, 2010, 2136
BsmFI	GGGAC	8 cut(s)	138, 443, 592, 769, 1211, 1835, 2095, 2818
BsmI	GAATGC	2 cut(s)	388, 1653
BsnI	GGCC	5 cut(s)	161, 466, 650, 1513, 1684
Bsp1286I	GDGCHC	3 cut(s)	216, 1735, 2421
BspANI	GGCC	5 cut(s)	161, 466, 650, 1513, 1684
BspCNI	CTCAG	6 cut(s)	409, 583, 595, 918, 2437, 2473
BspFNI	CGCG	1 cut(s)	21
BspLI	GGNNCC	5 cut(s)	875, 1210, 1543, 2083, 2084
BspMI	ACCTGC	2 cut(s)	1529, 1986
BspPI	GGATC	8 cut(s)	237, 269, 1203, 1216, 1812, 1893, 1980, 2639
BspT107I	GGYRCC	1 cut(s)	1541
BsrBI	CCGCTC	1 cut(s)	8
BsrDI	GCAATG	2 cut(s)	1107, 1765
BsrFI	RCCGGY	2 cut(s)	503, 2627
BsrI	ACTGG	7 cut(s)	634, 667, 719, 1696, 2169, 2255, 2266
BssAI	RCCGGY	2 cut(s)	503, 2627
BssECI	CCNNGG	4 cut(s)	156, 467, 1685, 2490
BssNAI	GTATAC	1 cut(s)	1777
BssNI	GRCGYC	1 cut(s)	151
BssSI	CACGAG	2 cut(s)	1458, 2403
BssT1I	CCWWGG	1 cut(s)	1685
Bst1107I	GTATAC	1 cut(s)	1777
Bst2BI	CACGAG	2 cut(s)	1458, 2403
Bst2UI	CCWGG	2 cut(s)	158, 1914
Bst4CI	ACNGT	4 cut(s)	1100, 1781, 2564, 2819
Bst6I	CTCTTC	2 cut(s)	53, 2103
BstACI	GRCGYC	1 cut(s)	151
BstBAI	YACGTR	1 cut(s)	1127
BstC8I	GCNNGC	5 cut(s)	414, 505, 1339, 1408, 1465
BstDSI	CCRYGG	1 cut(s)	2490
BstF5I	GGATG	8 cut(s)	382, 394, 492, 919, 1587, 1621, 1945, 2128
BstFNI	CGCG	1 cut(s)	21
BstHHI	GCGC	3 cut(s)	23, 708, 2270
BstMAI	GTCTC	4 cut(s)	573, 1793, 2010, 2136
BstMCI	CGRYCG	1 cut(s)	467
BstMWI	GCNNNNNNNGC	2 cut(s)	1233, 1242
BstNI	CCWGG	2 cut(s)	158, 1914
BstNSI	RCATGY	2 cut(s)	1714, 2011
BstPAI	GACNNNNGTC	2 cut(s)	2255, 2574
BstSCI	CCNGG	5 cut(s)	156, 276, 466, 1912, 2851
BstSFI	CTRYAG	2 cut(s)	1096, 2892
BstSLI	GKGCMC	2 cut(s)	1735, 2421
BstUI	CGCG	1 cut(s)	21
BstV1I	GCAGC	2 cut(s)	571, 1354
BstV2I	GAAGAC	3 cut(s)	327, 1277, 2703
BstX2I	RGATCY	4 cut(s)	575, 1208, 2098, 2644
BstXI	CCANNNNNNTGG	2 cut(s)	147, 1617
BstYI	RGATCY	4 cut(s)	575, 1208, 2098, 2644
BstZ17I	GTATAC	1 cut(s)	1777
BstZI	CGGCCG	1 cut(s)	464
BsuI	GTATCC	1 cut(s)	300
BsuRI	GGCC	5 cut(s)	161, 466, 650, 1513, 1684
BtgI	CCRYGG	1 cut(s)	2490
BtgZI	GCGATG	1 cut(s)	525
BtrI	CACGTC	2 cut(s)	30, 2728
BtsCI	GGATG	8 cut(s)	382, 394, 492, 919, 1587, 1621, 1945, 2128
BtsIMutI	CAGTG	5 cut(s)	502, 660, 1105, 1300, 2259
BveI	ACCTGC	2 cut(s)	1529, 1986
Cac8I	GCNNGC	5 cut(s)	414, 505, 1339, 1408, 1465
CaiI	CAGNNNCTG	1 cut(s)	2261
CfoI	GCGC	3 cut(s)	23, 708, 2270
Cfr10I	RCCGGY	2 cut(s)	503, 2627
Cfr13I	GGNCC	6 cut(s)	938, 952, 1028, 1382, 1512, 2082
CseI	GACGC	3 cut(s)	140, 890, 2027
Csp6I	GTAC	7 cut(s)	175, 196, 1137, 1221, 1818, 2113, 2468
CspAI	ACCGGT	1 cut(s)	2627
CspCI	CAANNNNNGTGG	2 cut(s)	1245, 1280
CviQI	GTAC	7 cut(s)	175, 196, 1137, 1221, 1818, 2113, 2468
DraI	TTTAAA	3 cut(s)	735, 1329, 2886
DraIII	CACNNNGTG	3 cut(s)	2408, 2584, 2934
DrdI	GACNNNNNNGTC	1 cut(s)	487
DriI	GACNNNNNGTC	1 cut(s)	1806
DseDI	GACNNNNNNGTC	1 cut(s)	487
EaeI	YGGCCR	2 cut(s)	464, 648
EagI	CGGCCG	1 cut(s)	464
Eam1104I	CTCTTC	2 cut(s)	53, 2103
Eam1105I	GACNNNNNGTC	1 cut(s)	1806
EarI	CTCTTC	2 cut(s)	53, 2103
EciI	GGCGGA	2 cut(s)	221, 514
Ecl136II	GAGCTC	1 cut(s)	214
EclXI	CGGCCG	1 cut(s)	464
Eco130I	CCWWGG	1 cut(s)	1685
Eco24I	GRGCYC	1 cut(s)	216
Eco32I	GATATC	1 cut(s)	1072
Eco47I	GGWCC	5 cut(s)	938, 952, 1028, 1382, 2082
Eco52I	CGGCCG	1 cut(s)	464
Eco53kI	GAGCTC	1 cut(s)	214
Eco57I	CTGAAG	3 cut(s)	1611, 1692, 1814
Eco72I	CACGTG	1 cut(s)	1127
EcoICRI	GAGCTC	1 cut(s)	214
EcoO109I	RGGNCCY	1 cut(s)	2082
EcoRI	GAATTC	1 cut(s)	666
EcoRII	CCWGG	2 cut(s)	156, 1912
EcoRV	GATATC	1 cut(s)	1072
EcoT14I	CCWWGG	1 cut(s)	1685
EcoT22I	ATGCAT	1 cut(s)	2377
EcoT38I	GRGCYC	1 cut(s)	216
ErhI	CCWWGG	1 cut(s)	1685
Esp3I	CGTCTC	3 cut(s)	1793, 2010, 2136
FalI	AAGNNNNNCTT	4 cut(s)	523, 555, 2668, 2700
FaqI	GGGAC	8 cut(s)	138, 443, 592, 769, 1211, 1835, 2095, 2818
FauI	CCCGC	2 cut(s)	690, 2518
FauNDI	CATATG	2 cut(s)	1189, 1432
FblI	GTMKAC	1 cut(s)	1776
Fnu4HI	GCNGC	5 cut(s)	24, 464, 508, 560, 1368
FokI	GGATG	8 cut(s)	389, 401, 499, 926, 1594, 1608, 1952, 2135
FriOI	GRGCYC	1 cut(s)	216
Fsp4HI	GCNGC	5 cut(s)	24, 464, 508, 560, 1368
FspBI	CTAG	5 cut(s)	1355, 1476, 1793, 1926, 2956
GlaI	GCGC	3 cut(s)	22, 707, 2269
GluI	GCNGC	5 cut(s)	24, 464, 508, 560, 1368
GsaI	CCCAGC	1 cut(s)	2025
GsuI	CTGGAG	6 cut(s)	571, 801, 1764, 1770, 1896, 2238
HaeIII	GGCC	5 cut(s)	161, 466, 650, 1513, 1684
HapII	CCGG	6 cut(s)	278, 467, 504, 2074, 2628, 2852
HgaI	GACGC	3 cut(s)	140, 890, 2027
HhaI	GCGC	3 cut(s)	23, 708, 2270
Hin1I	GRCGYC	1 cut(s)	151
Hin6I	GCGC	3 cut(s)	21, 706, 2268
HinP1I	GCGC	3 cut(s)	21, 706, 2268
HincII	GTYRAC	1 cut(s)	2869
HindII	GTYRAC	1 cut(s)	2869
HindIII	AAGCTT	3 cut(s)	1234, 1373, 2135
HpaII	CCGG	6 cut(s)	278, 467, 504, 2074, 2628, 2852
Hpy99I	CGWCG	3 cut(s)	56, 182, 298
HpyCH4III	ACNGT	4 cut(s)	1100, 1781, 2564, 2819
HpyCH4IV	ACGT	5 cut(s)	29, 177, 1126, 1599, 2727
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1233, 1242
HpySE526I	ACGT	5 cut(s)	29, 177, 1126, 1599, 2727
Hsp92I	GRCGYC	1 cut(s)	151
HspAI	GCGC	3 cut(s)	21, 706, 2268
KflI	GGGWCCC	1 cut(s)	2082
KroI	GCCGGC	1 cut(s)	503
KroNI	GCCGGC	1 cut(s)	505
LmnI	GCTCC	8 cut(s)	13, 211, 219, 357, 703, 814, 2241, 2305
Lsp1109I	GCAGC	2 cut(s)	571, 1354
LweI	GCATC	8 cut(s)	502, 778, 838, 1137, 1476, 1630, 2056, 2384
MaeI	CTAG	5 cut(s)	1355, 1476, 1793, 1926, 2956
MaeII	ACGT	5 cut(s)	29, 177, 1126, 1599, 2727
MbiI	CCGCTC	1 cut(s)	8
MfeI	CAATTG	2 cut(s)	897, 1014
MflI	RGATCY	4 cut(s)	575, 1208, 2098, 2644
MhlI	GDGCHC	3 cut(s)	216, 1735, 2421
MlsI	TGGCCA	1 cut(s)	650
MluNI	TGGCCA	1 cut(s)	650
MlyI	GAGTC	5 cut(s)	317, 614, 2245, 2470, 2777
MmeI	TCCRAC	1 cut(s)	317
Mox20I	TGGCCA	1 cut(s)	650
Mph1103I	ATGCAT	1 cut(s)	2377
MroNI	GCCGGC	1 cut(s)	503
MroXI	GAANNNNTTC	1 cut(s)	2136
MscI	TGGCCA	1 cut(s)	650
MseI	TTAA	9 cut(s)	734, 1328, 1560, 1719, 1902, 2154, 2352, 2670, 2885
MslI	CAYNNNNRTG	5 cut(s)	1001, 1158, 1250, 1615, 2207
Msp20I	TGGCCA	1 cut(s)	650
MspI	CCGG	6 cut(s)	278, 467, 504, 2074, 2628, 2852
MspR9I	CCNGG	5 cut(s)	158, 278, 468, 1914, 2853
MunI	CAATTG	2 cut(s)	897, 1014
Mva1269I	GAATGC	2 cut(s)	388, 1653
MvaI	CCWGG	2 cut(s)	158, 1914
MvnI	CGCG	1 cut(s)	21
MwoI	GCNNNNNNNGC	2 cut(s)	1233, 1242
NaeI	GCCGGC	1 cut(s)	505
NciI	CCSGG	3 cut(s)	278, 468, 2853
NdeI	CATATG	2 cut(s)	1189, 1432
NgoMIV	GCCGGC	1 cut(s)	503
NlaIV	GGNNCC	5 cut(s)	875, 1210, 1543, 2083, 2084
NmeAIII	GCCGAG	1 cut(s)	305
NmuCI	GTSAC	6 cut(s)	1100, 1122, 2257, 2576, 2584, 2780
NsiI	ATGCAT	1 cut(s)	2377
NspI	RCATGY	2 cut(s)	1714, 2011
OliI	CACNNNNGTG	2 cut(s)	1250, 2207
PaqCI	CACCTGC	2 cut(s)	1529, 1986
PciI	ACATGT	1 cut(s)	2007
PcsI	WCGNNNNNNNCGW	1 cut(s)	488
PctI	GAATGC	2 cut(s)	388, 1653
PdiI	GCCGGC	1 cut(s)	505
PdmI	GAANNNNTTC	1 cut(s)	2136
PfeI	GAWTC	9 cut(s)	544, 1427, 1642, 1931, 2220, 2329, 2498, 2555, 2951
PflMI	CCANNNNNTGG	4 cut(s)	1037, 2261, 2408, 2934
PinAI	ACCGGT	1 cut(s)	2627
PkrI	GCNGC	5 cut(s)	25, 465, 509, 561, 1369
PleI	GAGTC	5 cut(s)	317, 613, 2245, 2470, 2777
PmaCI	CACGTG	1 cut(s)	1127
PmlI	CACGTG	1 cut(s)	1127
PpsI	GAGTC	5 cut(s)	317, 613, 2245, 2470, 2777
Ppu21I	YACGTR	1 cut(s)	1127
PpuMI	RGGWCCY	1 cut(s)	2082
PscI	ACATGT	1 cut(s)	2007
PshAI	GACNNNNGTC	2 cut(s)	2255, 2574
PshBI	ATTAAT	1 cut(s)	2352
Psp124BI	GAGCTC	1 cut(s)	216
Psp5II	RGGWCCY	1 cut(s)	2082
Psp6I	CCWGG	2 cut(s)	156, 1912
PspCI	CACGTG	1 cut(s)	1127
PspFI	CCCAGC	1 cut(s)	2021
PspGI	CCWGG	2 cut(s)	156, 1912
PspN4I	GGNNCC	5 cut(s)	875, 1210, 1543, 2083, 2084
PspPI	GGNCC	6 cut(s)	938, 952, 1028, 1382, 1512, 2082
PspPPI	RGGWCCY	1 cut(s)	2082
PstNI	CAGNNNCTG	1 cut(s)	2261
PsuI	RGATCY	4 cut(s)	575, 1208, 2098, 2644
RsaI	GTAC	7 cut(s)	176, 197, 1138, 1222, 1819, 2114, 2469
RsaNI	GTAC	7 cut(s)	175, 196, 1137, 1221, 1818, 2113, 2468
RseI	CAYNNNNRTG	5 cut(s)	1001, 1158, 1250, 1615, 2207
SacI	GAGCTC	1 cut(s)	216
SaqAI	TTAA	9 cut(s)	734, 1328, 1560, 1719, 1902, 2154, 2352, 2670, 2885
SatI	GCNGC	5 cut(s)	24, 464, 508, 560, 1368
Sau96I	GGNCC	6 cut(s)	938, 952, 1028, 1382, 1512, 2082
ScaI	AGTACT	2 cut(s)	1819, 2114
SchI	GAGTC	5 cut(s)	317, 614, 2245, 2470, 2777
ScrFI	CCNGG	5 cut(s)	158, 278, 468, 1914, 2853
SduI	GDGCHC	3 cut(s)	216, 1735, 2421
SfaNI	GCATC	8 cut(s)	502, 778, 838, 1137, 1476, 1630, 2056, 2384
SfcI	CTRYAG	2 cut(s)	1096, 2892
SgrAI	CRCCGGYG	1 cut(s)	2627
SinI	GGWCC	5 cut(s)	938, 952, 1028, 1382, 2082
SmiMI	CAYNNNNRTG	5 cut(s)	1001, 1158, 1250, 1615, 2207
SmlI	CTYRAG	3 cut(s)	579, 1442, 2605
SmoI	CTYRAG	3 cut(s)	579, 1442, 2605
SpeI	ACTAGT	1 cut(s)	1354
SspI	AATATT	2 cut(s)	1900, 2616
SspMI	CTAG	5 cut(s)	1355, 1476, 1793, 1926, 2956
SstI	GAGCTC	1 cut(s)	216
StyD4I	CCNGG	5 cut(s)	156, 276, 466, 1912, 2851
StyI	CCWWGG	1 cut(s)	1685
TaaI	ACNGT	4 cut(s)	1100, 1781, 2564, 2819
TaiI	ACGT	5 cut(s)	32, 180, 1129, 1602, 2730
TaqI	TCGA	9 cut(s)	184, 264, 450, 542, 670, 1868, 2034, 2140, 2368
TatI	WGTACW	6 cut(s)	195, 1136, 1220, 1817, 2112, 2467
TauI	GCSGC	3 cut(s)	26, 466, 510
TfiI	GAWTC	9 cut(s)	544, 1427, 1642, 1931, 2220, 2329, 2498, 2555, 2951
Tru1I	TTAA	9 cut(s)	734, 1328, 1560, 1719, 1902, 2154, 2352, 2670, 2885
Tru9I	TTAA	9 cut(s)	734, 1328, 1560, 1719, 1902, 2154, 2352, 2670, 2885
TscAI	CASTG	5 cut(s)	502, 667, 1105, 1300, 2266
TseFI	GTSAC	6 cut(s)	1100, 1122, 2257, 2576, 2584, 2780
TseI	GCWGC	2 cut(s)	559, 1367
Tsp45I	GTSAC	6 cut(s)	1100, 1122, 2257, 2576, 2584, 2780
TspDTI	ATGAA	4 cut(s)	579, 738, 1358, 1449
TspGWI	ACGGA	8 cut(s)	311, 1041, 1508, 1817, 1934, 2254, 2399, 2776
TspRI	CASTG	5 cut(s)	502, 667, 1105, 1300, 2266
Van91I	CCANNNNNTGG	4 cut(s)	1037, 2261, 2408, 2934
VneI	GTGCAC	1 cut(s)	2417
VpaK11BI	GGWCC	5 cut(s)	938, 952, 1028, 1382, 2082
VspI	ATTAAT	1 cut(s)	2352
XapI	RAATTY	5 cut(s)	142, 666, 1987, 2731, 2887
XceI	RCATGY	2 cut(s)	1714, 2011
XmiI	GTMKAC	1 cut(s)	1776
XmnI	GAANNNNTTC	1 cut(s)	2136
XspI	CTAG	5 cut(s)	1355, 1476, 1793, 1926, 2956
ZrmI	AGTACT	2 cut(s)	1819, 2114
Zsp2I	ATGCAT	1 cut(s)	2377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.