Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Reverse (-)

88611951 .. 88617199

Gene Structure 5249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00151320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3117 bp

ATGACCACTCCTCTGACCGCCACGTCATCACTCTCCTTCTTCATCTTCATCATCGTCTCCATCGCCGTAGCCGTCTCCATCTCTCTCTTCCATTACAAACCCCACCAGAACCCCCCAACCCTAGAGCCCAGCGCAACCCTAGATCCTCCTCCACCGCAAAACTACGTCGTTCGATTCGTCGAGTACAAGAGGTGGGAGGATCACCGGGAGTATCTGAAATCGACCGTGCCGGCGGAGGGATGGGACTGGATCGAGAGGAACAATCCGGCCATGGATTACCCGACGGATTTCGGGCTCGTCCGGATTGACGATGGAGCCAGGGAATTGGTGATTGGGGAGATTGGGAAATTGGGGATGGTGAAGGATGTGAATGCTGACGTCAGGTATGGGAGGGGTTTGCTTAGTGAGGAGGAGAAGAGGGAGAAAGTTGGGGCCTTTGTGGATGGGGAGAAGCGGCCGGGAAAGATGTTCACGGCGATGTCGTTTAGCGAAGACGACGGAGCGGTAGTGAGTAATGGCTCTGTTAGCTGGAAAAGGGAGCTCTTGGCTGAGAGATCTCAAATAACTTCATTGTTTGGAGCGGATTTTCTTTGGACAAAAGGGTATACCGGTACGAAAGTGAAAATGGCCATATTTGATACTGGTATTCGAGCTAATCACCCGCACTTTCGAAATATTAAGGAGCGGACAAACTGGACAAATGAGGATACATTAAACGACAATCTTGGACATGGGACATTTGTTGCTGGTGTTGTTGCTGGTGTAGATGCAGAGTGTCTTGGATTTGCTCCTGATACAGAGATATATGCGTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGATGCATTCAACTATGCCATTGCGACCAATATGGATGTACTGAACTTGAGCATAGGTGGACCTGATTACTTGGACCTTCCATTTGTGGAGAAGGTCTGGGAAATAACAGCAAACAACATAATTATGGTTTCAGCAATTGGAAACGATGGACCACTTTATGGGACCCTAAATAATCCAGCAGACCAAAGTGATGTCATTGGTGTTGGTGGCATCGACTACAGTGATCACATTGCTTCTTTTTCCTCACGAGGCATGAGTACTTGGGAGATGCCGCATGGTTATGGTCGTGTTAAGCCAGATGTCGTTGCATATGGACGGGAAATTATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCTGGCACTAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTTTGTCTGCTTGTGAGTGTTATCCCTGAAAGCAGTCGGAAGGAGACTTTAAATCCAGCAAGCATGAAACAAGCATTGGTTGAGGGTGCTGCAAAACTCGGTGGTCCAAATATGTATGAGCAGGGTGCAGGGAGGGTTGATCTGTTAGAATCATATGAAATCCTGAAAAATTACCAACCTCGTGCCAGCATCTTCCCTAGTATTCTTGATTATACAGACTGCCCATACTCTTGGCCCTTCTGTCGTCAGCCACTTTATGCTGGTGCCATGCCTGTTATCTTCAACGCTACCATTCTAAATGGAATGGGCGTAATTGGCTATGTTGAAAGTCCACCAACTTGGCATCCTTCAGATGAAGTAGGGAATCTTCTAAGTATTCACTTTACATATTCTAATGTTATCTGGCCTTGGACTGGTTATTTAGCGCTTCACATGCAAATTAAGGACGAAGGTGCACAATTTTCAGGAGAGATTAGCGGCAATGTTACTCTTAGGGTGTACAGTCCTCCATCTCCGGGAGAAAAGAATCTTCGGATTAGCACTTGTGTGCTTCAGTTAAGATTGAAGATTGTTCCAACTCCACCAAGATCAAAACGAGTTTTGTGGGACCAGTTTCACAGTATTAAATACCCTCCAGGTTACATCCCTAGAGACTCTTTGGATGTTCGCAATGACATTCTTGACTGGCATGGAGATCACCTGCATACAAATTTTCACATTATGTTTAACATGTTACGAGATGCTGGGTATTATGTAGAAACACTTGGTTCGCCTCTTACTTGCTTTGATGCTCTTCGATATGGGACACTTCTTCTGGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAGAAGCTGCGAGATGATGTTCTTAATTCTGGACTGGGATTGGTTGTGTTTTCTGAGTGGTATAATGTAGACACGATGGTGAAGATGAGATTCTTTGATGATAACACACGTAGCTGGTGGACTCCAGTCACTGGAGGTGCAAATGTTCCAGCGTTGAACGACCTGCTGGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATCAATGGTGAGCAAAGTCGGTATGCATCTGGAACAGATATTGTGAGGTTTCCGAAAGGGGGTTATGTACACAAGTTCCCTTTCCTGGATAGCTCTGAAAGTGGGGCCACTCAGAATGTACTTCGGGATCCTGAGGTGACAATGGCAGATTCTCCCATTCTTGGTCTTGTACAGGTGGGTGAAGGTCGTGTTGTAGTGTATGGAGATTCTAACTGTTTGGACAGCAGTCATATGGTAACTAATTGTTATTGGCTCTTGAGAAAAATGCTAGATTTCACCGGTGGAAACATCAGAGATCCTGTGCTTTTTTCAAGCTCGGTCAAACAAAGTTCACCTTTATATATGGAAGATAACCAATTACCGTCTCGTAGAACAGATGTGAATTTTTCTACATATTCCGCTGTAGTGGGGAAGGAATTGATCTGTGGGAGTGACTCTGTATTTGAAATATGGGGGACTAAAGGTTACAGTTTACAGGTCAGGGGAACAAACAGAAGATTACCAGGCTCTTCTGTGTCTAATCCAGGTAGAGGTTTGAATTCTACTGTCGAGGCTTCCCATTTGAAGCATTCCGAGTCAACACTGGGAAATAGAAGTGATCCTTCGGGATACTTGGGAATAATCTATACGGATGAGCTTGATATGCCTGTGTTAGTTGCTAGTCACTGGCTTGTTCCTGCAATAATTGCCGTAACTGGTTTGGTGGTATTTTTGAGCTTCTGGAGAATTAGACAGAAGCGACGACGACGAAGAAAAGGATCTAGTTCTGGTCGATATTCTAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

1038

Amino Acids

115.29

Weight (kDa)

5.47

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	53 - 125	5.7e-13	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	204 - 456	8.2e-42	Subtilase family
MBTPS1_3rd	PF23094	484 - 612	2.7e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	613 - 884	2.3e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1965
AasI	GACNNNNNNGTC	1 cut(s)	22
AatII	GACGTC	1 cut(s)	381
Acc36I	ACCTGC	2 cut(s)	1965, 2280
AccB1I	GGYRCC	1 cut(s)	1520
AccB7I	CCANNNNNTGG	2 cut(s)	1016, 2240
AccBSI	CCGCTC	3 cut(s)	503, 581, 685
AccI	GTMKAC	2 cut(s)	605, 2178
AccIII	TCCGGA	1 cut(s)	300
AcoI	YGGCCR	3 cut(s)	267, 455, 627
AcsI	RAATTY	3 cut(s)	1966, 2710, 2866
AcuI	CTGAAG	2 cut(s)	1590, 1793
AcyI	GRCGYC	1 cut(s)	378
AfeI	AGCGCT	1 cut(s)	1683
AfiI	CCNNNNNNNGG	9 cut(s)	236, 1016, 1670, 1772, 2240, 2387, 2413, 2489, 2858
AflIII	ACRYGT	2 cut(s)	1986, 2216
AgeI	ACCGGT	2 cut(s)	608, 2606
AhlI	ACTAGT	1 cut(s)	1226
AjiI	CACGTC	1 cut(s)	24
AjnI	CCWGG	5 cut(s)	317, 1891, 2412, 2830, 2851
AjuI	GAANNNNNNNTTGG	4 cut(s)	315, 347, 1316, 1348
AleI	CACNNNNGTG	3 cut(s)	1229, 1802, 2186
Alw21I	GWGCWC	2 cut(s)	543, 1714
Alw26I	GTCTC	5 cut(s)	61, 79, 1295, 1902, 2697
Alw44I	GTGCAC	1 cut(s)	1710
AlwNI	CAGNNNCTG	1 cut(s)	2240
Aor13HI	TCCGGA	1 cut(s)	300
Aor51HI	AGCGCT	1 cut(s)	1683
AoxI	GGCC	7 cut(s)	267, 432, 455, 627, 1490, 1661, 2433
ApaLI	GTGCAC	1 cut(s)	1710
ApeKI	GCWGC	2 cut(s)	1346, 2116
ApoI	RAATTY	3 cut(s)	1966, 2710, 2866
ArsI	GACNNNNNNTTYG	4 cut(s)	923, 955, 2753, 2785
AsiGI	ACCGGT	2 cut(s)	608, 2606
AspLEI	GCGC	2 cut(s)	134, 1684
AspS9I	GGNCC	9 cut(s)	432, 917, 931, 1007, 1020, 1361, 1491, 1864, 2433
AsuC2I	CCSGG	3 cut(s)	206, 459, 1773
AsuII	TTCGAA	1 cut(s)	670
AvaII	GGWCC	6 cut(s)	917, 931, 1007, 1020, 1361, 1864
AxyI	CCTNAGG	1 cut(s)	2460
BaeGI	GKGCMC	1 cut(s)	1714
BaeI	ACNNNNGTAYC	2 cut(s)	603, 636
BalI	TGGCCA	1 cut(s)	629
BamHI	GGATCC	2 cut(s)	1187, 2455
BanI	GGYRCC	1 cut(s)	1520
BanII	GRGCYC	3 cut(s)	129, 297, 543
BarI	GAAGNNNNNNTAC	2 cut(s)	1608, 1640
BauI	CACGAG	2 cut(s)	1104, 1437
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	2 cut(s)	543, 1714
BbvI	GCAGC	2 cut(s)	1333, 2103
BccI	CCATC	9 cut(s)	68, 86, 234, 305, 349, 437, 998, 1774, 2179
BceAI	ACGGC	4 cut(s)	50, 56, 489, 3002
BciT130I	CCWGG	5 cut(s)	319, 1893, 2414, 2832, 2853
BciVI	GTATCC	2 cut(s)	700, 2930
BclI	TGATCA	1 cut(s)	1081
BcnI	CCSGG	3 cut(s)	206, 459, 1773
BcoDI	GTCTC	5 cut(s)	61, 79, 1295, 1902, 2697
BcuI	ACTAGT	1 cut(s)	1226
BfaI	CTAG	8 cut(s)	122, 140, 1227, 1455, 1905, 2597, 2988, 3090
BfmI	CTRYAG	2 cut(s)	1075, 2730
BfoI	RGCGCY	1 cut(s)	1685
BfuAI	ACCTGC	2 cut(s)	1965, 2280
BfuI	GTATCC	2 cut(s)	700, 2930
BglII	AGATCT	2 cut(s)	554, 2077
BisI	GCNGC	5 cut(s)	455, 1130, 1347, 1735, 2117
BlsI	GCNGC	5 cut(s)	456, 1131, 1348, 1736, 2118
BmcAI	AGTACT	2 cut(s)	1117, 2093
Bme1390I	CCNGG	8 cut(s)	206, 319, 459, 1773, 1893, 2414, 2832, 2853
Bme18I	GGWCC	6 cut(s)	917, 931, 1007, 1020, 1361, 1864
BmgBI	CACGTC	1 cut(s)	24
BmgT120I	GGNCC	9 cut(s)	432, 917, 931, 1007, 1020, 1361, 1491, 1864, 2433
BmrFI	CCNGG	8 cut(s)	206, 319, 459, 1773, 1893, 2414, 2832, 2853
BmrI	ACTGGG	2 cut(s)	2153, 2921
BmuI	ACTGGG	2 cut(s)	2153, 2921
BoxI	GACNNNNGTC	2 cut(s)	2234, 2553
BpiI	GAAGAC	1 cut(s)	498
BpmI	CTGGAG	4 cut(s)	1875, 2217, 2262, 3070
Bpu14I	TTCGAA	1 cut(s)	670
BpuEI	CTTGAG	2 cut(s)	925, 2605
BpuMI	CCSGG	3 cut(s)	206, 459, 1773
BsaAI	YACGTR	1 cut(s)	2219
BsaHI	GRCGYC	1 cut(s)	378
BsaJI	CCNNGG	3 cut(s)	270, 318, 1664
BsaWI	WCCGGW	3 cut(s)	300, 608, 2606
BsaXI	ACNNNNNCTCC	2 cut(s)	492, 522
Bsc4I	CCNNNNNNNGG	9 cut(s)	236, 1016, 1670, 1772, 2240, 2387, 2413, 2489, 2858
Bse118I	RCCGGY	3 cut(s)	229, 608, 2606
Bse21I	CCTNAGG	1 cut(s)	2460
Bse3DI	GCAATG	4 cut(s)	876, 1086, 1744, 1933
BseAI	TCCGGA	1 cut(s)	300
BseBI	CCWGG	5 cut(s)	319, 1893, 2414, 2832, 2853
BseDI	CCNNGG	3 cut(s)	270, 318, 1664
BseGI	GGATG	9 cut(s)	245, 360, 370, 448, 898, 1600, 1899, 1924, 2965
BseLI	CCNNNNNNNGG	9 cut(s)	236, 1016, 1670, 1772, 2240, 2387, 2413, 2489, 2858
BseMI	GCAATG	4 cut(s)	876, 1086, 1744, 1933
BseMII	CTCAG	4 cut(s)	540, 2154, 2451, 2453
BseRI	GAGGAG	4 cut(s)	138, 422, 425, 2117
BseSI	GKGCMC	1 cut(s)	1714
BseX3I	CGGCCG	1 cut(s)	455
BseXI	GCAGC	2 cut(s)	1333, 2103
BseYI	CCCAGC	2 cut(s)	128, 2000
BsgI	GTGCAG	1 cut(s)	1404
Bsh1285I	CGRYCG	2 cut(s)	225, 458
BshFI	GGCC	7 cut(s)	269, 434, 457, 629, 1492, 1663, 2435
BshNI	GGYRCC	1 cut(s)	1520
BshTI	ACCGGT	2 cut(s)	608, 2606
BsiEI	CGRYCG	2 cut(s)	225, 458
BsiHKAI	GWGCWC	2 cut(s)	543, 1714
BsiSI	CCGG	8 cut(s)	205, 230, 266, 301, 458, 609, 1772, 2607
BslFI	GGGAC	6 cut(s)	257, 748, 1033, 1877, 2074, 2797
BslI	CCNNNNNNNGG	9 cut(s)	236, 1016, 1670, 1772, 2240, 2387, 2413, 2489, 2858
BsmAI	GTCTC	5 cut(s)	61, 79, 1295, 1902, 2697
BsmBI	CGTCTC	3 cut(s)	61, 79, 2697
BsmFI	GGGAC	6 cut(s)	257, 748, 1033, 1877, 2074, 2797
BsmI	GAATGC	3 cut(s)	376, 863, 2896
BsnI	GGCC	7 cut(s)	269, 434, 457, 629, 1492, 1663, 2435
Bsp119I	TTCGAA	1 cut(s)	670
Bsp1286I	GDGCHC	4 cut(s)	129, 297, 543, 1714
Bsp13I	TCCGGA	1 cut(s)	300
Bsp1407I	TGTACA	3 cut(s)	1755, 2395, 2497
Bsp19I	CCATGG	1 cut(s)	270
BspANI	GGCC	7 cut(s)	269, 434, 457, 629, 1492, 1663, 2435
BspCNI	CTCAG	4 cut(s)	541, 2155, 2452, 2452
BspEI	TCCGGA	1 cut(s)	300
BspMI	ACCTGC	2 cut(s)	1965, 2280
BspQI	GCTCTTC	2 cut(s)	2055, 2842
BspT104I	TTCGAA	1 cut(s)	670
BspT107I	GGYRCC	1 cut(s)	1520
BsrBI	CCGCTC	3 cut(s)	503, 581, 685
BsrDI	GCAATG	4 cut(s)	876, 1086, 1744, 1933
BsrFI	RCCGGY	3 cut(s)	229, 608, 2606
BsrGI	TGTACA	3 cut(s)	1755, 2395, 2497
BssAI	RCCGGY	3 cut(s)	229, 608, 2606
BssECI	CCNNGG	3 cut(s)	270, 318, 1664
BssNAI	GTATAC	1 cut(s)	606
BssNI	GRCGYC	1 cut(s)	378
BssSI	CACGAG	2 cut(s)	1104, 1437
BssT1I	CCWWGG	2 cut(s)	270, 1664
Bst1107I	GTATAC	1 cut(s)	606
Bst2BI	CACGAG	2 cut(s)	1104, 1437
Bst2UI	CCWGG	5 cut(s)	319, 1893, 2414, 2832, 2853
Bst4CI	ACNGT	8 cut(s)	226, 1079, 1760, 1877, 2543, 2691, 2798, 2875
Bst6I	CTCTTC	5 cut(s)	92, 410, 2055, 2082, 2842
BstACI	GRCGYC	1 cut(s)	378
BstAPI	GCANNNNNTGC	1 cut(s)	3014
BstAUI	TGTACA	3 cut(s)	1755, 2395, 2497
BstBAI	YACGTR	1 cut(s)	2219
BstBI	TTCGAA	1 cut(s)	670
BstC8I	GCNNGC	4 cut(s)	231, 1318, 1444, 2277
BstDEI	CTNAG	7 cut(s)	401, 549, 1628, 1748, 2163, 2439, 2460
BstDSI	CCRYGG	1 cut(s)	270
BstF5I	GGATG	9 cut(s)	245, 360, 370, 448, 898, 1600, 1899, 1924, 2965
BstH2I	RGCGCY	1 cut(s)	1685
BstHHI	GCGC	2 cut(s)	134, 1684
BstMAI	GTCTC	5 cut(s)	61, 79, 1295, 1902, 2697
BstMCI	CGRYCG	2 cut(s)	225, 458
BstMWI	GCNNNNNNNGC	7 cut(s)	525, 1212, 1221, 1572, 1690, 2971, 3014
BstNI	CCWGG	5 cut(s)	319, 1893, 2414, 2832, 2853
BstNSI	RCATGY	2 cut(s)	1693, 1990
BstPAI	GACNNNNGTC	2 cut(s)	2234, 2553
BstSCI	CCNGG	8 cut(s)	204, 317, 457, 1771, 1891, 2412, 2830, 2851
BstSFI	CTRYAG	2 cut(s)	1075, 2730
BstSLI	GKGCMC	1 cut(s)	1714
BstV1I	GCAGC	2 cut(s)	1333, 2103
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	7 cut(s)	142, 554, 1187, 2077, 2455, 2623, 3086
BstXI	CCANNNNNNTGG	3 cut(s)	325, 1488, 1596
BstYI	RGATCY	7 cut(s)	142, 554, 1187, 2077, 2455, 2623, 3086
BstZ17I	GTATAC	1 cut(s)	606
BstZI	CGGCCG	1 cut(s)	455
Bsu36I	CCTNAGG	1 cut(s)	2460
BsuI	GTATCC	2 cut(s)	700, 2930
BsuRI	GGCC	7 cut(s)	269, 434, 457, 629, 1492, 1663, 2435
BtgI	CCRYGG	1 cut(s)	270
BtgZI	GCGATG	2 cut(s)	46, 491
BtrI	CACGTC	1 cut(s)	24
BtsCI	GGATG	9 cut(s)	245, 360, 370, 448, 898, 1600, 1899, 1924, 2965
BtsIMutI	CAGTG	4 cut(s)	1084, 2238, 2909, 2992
BveI	ACCTGC	2 cut(s)	1965, 2280
Cac8I	GCNNGC	4 cut(s)	231, 1318, 1444, 2277
CaiI	CAGNNNCTG	1 cut(s)	2240
CfoI	GCGC	2 cut(s)	134, 1684
Cfr10I	RCCGGY	3 cut(s)	229, 608, 2606
Cfr13I	GGNCC	9 cut(s)	432, 917, 931, 1007, 1020, 1361, 1491, 1864, 2433
CspAI	ACCGGT	2 cut(s)	608, 2606
CspCI	CAANNNNNGTGG	2 cut(s)	1224, 1259
DdeI	CTNAG	7 cut(s)	401, 549, 1628, 1748, 2163, 2439, 2460
DraI	TTTAAA	1 cut(s)	1308
DrdI	GACNNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	3 cut(s)	267, 455, 627
EagI	CGGCCG	1 cut(s)	455
Eam1104I	CTCTTC	5 cut(s)	92, 410, 2055, 2082, 2842
EarI	CTCTTC	5 cut(s)	92, 410, 2055, 2082, 2842
EciI	GGCGGA	1 cut(s)	248
Ecl136II	GAGCTC	1 cut(s)	541
EclXI	CGGCCG	1 cut(s)	455
Eco130I	CCWWGG	2 cut(s)	270, 1664
Eco24I	GRGCYC	3 cut(s)	129, 297, 543
Eco47I	GGWCC	6 cut(s)	917, 931, 1007, 1020, 1361, 1864
Eco47III	AGCGCT	1 cut(s)	1683
Eco52I	CGGCCG	1 cut(s)	455
Eco53kI	GAGCTC	1 cut(s)	541
Eco57I	CTGAAG	2 cut(s)	1590, 1793
Eco81I	CCTNAGG	1 cut(s)	2460
EcoICRI	GAGCTC	1 cut(s)	541
EcoO109I	RGGNCCY	2 cut(s)	432, 1020
EcoRI	GAATTC	1 cut(s)	2866
EcoRII	CCWGG	5 cut(s)	317, 1891, 2412, 2830, 2851
EcoT14I	CCWWGG	2 cut(s)	270, 1664
EcoT22I	ATGCAT	2 cut(s)	865, 2356
EcoT38I	GRGCYC	3 cut(s)	129, 297, 543
ErhI	CCWWGG	2 cut(s)	270, 1664
Esp3I	CGTCTC	3 cut(s)	61, 79, 2697
FalI	AAGNNNNNCTT	4 cut(s)	2647, 2679, 2914, 2946
FaqI	GGGAC	6 cut(s)	257, 748, 1033, 1877, 2074, 2797
FauI	CCCGC	1 cut(s)	669
FauNDI	CATATG	3 cut(s)	1168, 1411, 2559
FbaI	TGATCA	1 cut(s)	1081
FblI	GTMKAC	2 cut(s)	605, 2178
Fnu4HI	GCNGC	5 cut(s)	455, 1130, 1347, 1735, 2117
FokI	GGATG	9 cut(s)	252, 367, 377, 455, 905, 1587, 1886, 1931, 2972
FriOI	GRGCYC	3 cut(s)	129, 297, 543
Fsp4HI	GCNGC	5 cut(s)	455, 1130, 1347, 1735, 2117
FspBI	CTAG	8 cut(s)	122, 140, 1227, 1455, 1905, 2597, 2988, 3090
GlaI	GCGC	2 cut(s)	133, 1683
GluI	GCNGC	5 cut(s)	455, 1130, 1347, 1735, 2117
GsaI	CCCAGC	2 cut(s)	132, 2004
GsuI	CTGGAG	4 cut(s)	1875, 2217, 2262, 3070
HaeII	RGCGCY	1 cut(s)	1685
HaeIII	GGCC	7 cut(s)	269, 434, 457, 629, 1492, 1663, 2435
HapII	CCGG	8 cut(s)	205, 230, 266, 301, 458, 609, 1772, 2607
HhaI	GCGC	2 cut(s)	134, 1684
Hin1I	GRCGYC	1 cut(s)	378
Hin6I	GCGC	2 cut(s)	132, 1682
HinP1I	GCGC	2 cut(s)	132, 1682
HincII	GTYRAC	1 cut(s)	2907
HindII	GTYRAC	1 cut(s)	2907
HindIII	AAGCTT	1 cut(s)	1213
HpaII	CCGG	8 cut(s)	205, 230, 266, 301, 458, 609, 1772, 2607
Hpy99I	CGWCG	7 cut(s)	170, 182, 286, 500, 3072, 3075, 3078
HpyCH4III	ACNGT	8 cut(s)	226, 1079, 1760, 1877, 2543, 2691, 2798, 2875
HpyCH4IV	ACGT	4 cut(s)	23, 165, 378, 2218
HpyF10VI	GCNNNNNNNGC	7 cut(s)	525, 1212, 1221, 1572, 1690, 2971, 3014
HpyF3I	CTNAG	7 cut(s)	401, 549, 1628, 1748, 2163, 2439, 2460
HpySE526I	ACGT	4 cut(s)	23, 165, 378, 2218
Hsp92I	GRCGYC	1 cut(s)	378
HspAI	GCGC	2 cut(s)	132, 1682
KflI	GGGWCCC	1 cut(s)	1020
Kpn2I	TCCGGA	1 cut(s)	300
KroI	GCCGGC	1 cut(s)	229
KroNI	GCCGGC	1 cut(s)	231
Ksp22I	TGATCA	1 cut(s)	1081
LguI	GCTCTTC	2 cut(s)	2055, 2842
LmnI	GCTCC	7 cut(s)	314, 500, 538, 578, 682, 793, 2284
Lsp1109I	GCAGC	2 cut(s)	1333, 2103
MaeI	CTAG	8 cut(s)	122, 140, 1227, 1455, 1905, 2597, 2988, 3090
MaeII	ACGT	4 cut(s)	23, 165, 378, 2218
MbiI	CCGCTC	3 cut(s)	503, 581, 685
MfeI	CAATTG	1 cut(s)	993
MflI	RGATCY	7 cut(s)	142, 554, 1187, 2077, 2455, 2623, 3086
MhlI	GDGCHC	4 cut(s)	129, 297, 543, 1714
MlsI	TGGCCA	1 cut(s)	629
MluNI	TGGCCA	1 cut(s)	629
MlyI	GAGTC	4 cut(s)	1904, 2224, 2756, 2912
MmeI	TCCRAC	2 cut(s)	1274, 1856
Mox20I	TGGCCA	1 cut(s)	629
Mph1103I	ATGCAT	2 cut(s)	865, 2356
MroI	TCCGGA	1 cut(s)	300
MroNI	GCCGGC	1 cut(s)	229
MscI	TGGCCA	1 cut(s)	629
MseI	TTAA	9 cut(s)	678, 713, 1149, 1307, 1698, 1814, 1881, 1983, 2133
MslI	CAYNNNNRTG	6 cut(s)	476, 980, 1137, 1229, 1802, 2186
Msp20I	TGGCCA	1 cut(s)	629
MspA1I	CMGCKG	1 cut(s)	2729
MspI	CCGG	8 cut(s)	205, 230, 266, 301, 458, 609, 1772, 2607
MspR9I	CCNGG	8 cut(s)	206, 319, 459, 1773, 1893, 2414, 2832, 2853
MunI	CAATTG	1 cut(s)	993
Mva1269I	GAATGC	3 cut(s)	376, 863, 2896
MvaI	CCWGG	5 cut(s)	319, 1893, 2414, 2832, 2853
MwoI	GCNNNNNNNGC	7 cut(s)	525, 1212, 1221, 1572, 1690, 2971, 3014
NaeI	GCCGGC	1 cut(s)	231
NciI	CCSGG	3 cut(s)	206, 459, 1773
NcoI	CCATGG	1 cut(s)	270
NdeI	CATATG	3 cut(s)	1168, 1411, 2559
NgoMIV	GCCGGC	1 cut(s)	229
NmuCI	GTSAC	4 cut(s)	2236, 2464, 2759, 2990
NsiI	ATGCAT	2 cut(s)	865, 2356
NspI	RCATGY	2 cut(s)	1693, 1990
NspV	TTCGAA	1 cut(s)	670
OliI	CACNNNNGTG	3 cut(s)	1229, 1802, 2186
PaqCI	CACCTGC	1 cut(s)	1965
PciI	ACATGT	1 cut(s)	1986
PciSI	GCTCTTC	2 cut(s)	2055, 2842
PcsI	WCGNNNNNNNCGW	3 cut(s)	69, 174, 479
PctI	GAATGC	3 cut(s)	376, 863, 2896
PdiI	GCCGGC	1 cut(s)	231
PfeI	GAWTC	8 cut(s)	174, 1406, 1621, 1783, 2199, 2308, 2477, 2534
PflMI	CCANNNNNTGG	2 cut(s)	1016, 2240
PfoI	TCCNGGA	2 cut(s)	1771, 2412
PinAI	ACCGGT	2 cut(s)	608, 2606
PkrI	GCNGC	5 cut(s)	456, 1131, 1348, 1736, 2118
PleI	GAGTC	4 cut(s)	1904, 2224, 2756, 2911
PpsI	GAGTC	4 cut(s)	1904, 2224, 2756, 2911
Ppu21I	YACGTR	1 cut(s)	2219
PpuMI	RGGWCCY	1 cut(s)	1020
PscI	ACATGT	1 cut(s)	1986
PshAI	GACNNNNGTC	2 cut(s)	2234, 2553
Psp124BI	GAGCTC	1 cut(s)	543
Psp5II	RGGWCCY	1 cut(s)	1020
Psp6I	CCWGG	5 cut(s)	317, 1891, 2412, 2830, 2851
PspFI	CCCAGC	2 cut(s)	128, 2000
PspGI	CCWGG	5 cut(s)	317, 1891, 2412, 2830, 2851
PspPI	GGNCC	9 cut(s)	432, 917, 931, 1007, 1020, 1361, 1491, 1864, 2433
PspPPI	RGGWCCY	1 cut(s)	1020
PstNI	CAGNNNCTG	1 cut(s)	2240
PsuI	RGATCY	7 cut(s)	142, 554, 1187, 2077, 2455, 2623, 3086
RseI	CAYNNNNRTG	6 cut(s)	476, 980, 1137, 1229, 1802, 2186
SacI	GAGCTC	1 cut(s)	543
SapI	GCTCTTC	2 cut(s)	2055, 2842
SaqAI	TTAA	9 cut(s)	678, 713, 1149, 1307, 1698, 1814, 1881, 1983, 2133
SatI	GCNGC	5 cut(s)	455, 1130, 1347, 1735, 2117
Sau96I	GGNCC	9 cut(s)	432, 917, 931, 1007, 1020, 1361, 1491, 1864, 2433
ScaI	AGTACT	2 cut(s)	1117, 2093
SchI	GAGTC	4 cut(s)	1904, 2224, 2756, 2912
ScrFI	CCNGG	8 cut(s)	206, 319, 459, 1773, 1893, 2414, 2832, 2853
SduI	GDGCHC	4 cut(s)	129, 297, 543, 1714
SfcI	CTRYAG	2 cut(s)	1075, 2730
SfuI	TTCGAA	1 cut(s)	670
SgrAI	CRCCGGYG	1 cut(s)	2606
SinI	GGWCC	6 cut(s)	917, 931, 1007, 1020, 1361, 1864
SmiMI	CAYNNNNRTG	6 cut(s)	476, 980, 1137, 1229, 1802, 2186
SmlI	CTYRAG	2 cut(s)	904, 2584
SmoI	CTYRAG	2 cut(s)	904, 2584
SpeI	ACTAGT	1 cut(s)	1226
SspI	AATATT	1 cut(s)	676
SspMI	CTAG	8 cut(s)	122, 140, 1227, 1455, 1905, 2597, 2988, 3090
SstI	GAGCTC	1 cut(s)	543
StyD4I	CCNGG	8 cut(s)	204, 317, 457, 1771, 1891, 2412, 2830, 2851
StyI	CCWWGG	2 cut(s)	270, 1664
TaaI	ACNGT	8 cut(s)	226, 1079, 1760, 1877, 2543, 2691, 2798, 2875
TaiI	ACGT	4 cut(s)	26, 168, 381, 2221
TaqII	GACCGA	1 cut(s)	2635
TatI	WGTACW	9 cut(s)	183, 895, 1115, 1199, 1755, 2091, 2395, 2446, 2497
TauI	GCSGC	3 cut(s)	457, 1132, 1737
TfiI	GAWTC	8 cut(s)	174, 1406, 1621, 1783, 2199, 2308, 2477, 2534
Tru1I	TTAA	9 cut(s)	678, 713, 1149, 1307, 1698, 1814, 1881, 1983, 2133
Tru9I	TTAA	9 cut(s)	678, 713, 1149, 1307, 1698, 1814, 1881, 1983, 2133
TscAI	CASTG	4 cut(s)	1084, 2245, 2916, 2999
TseFI	GTSAC	4 cut(s)	2236, 2464, 2759, 2990
TseI	GCWGC	2 cut(s)	1346, 2116
Tsp45I	GTSAC	4 cut(s)	2236, 2464, 2759, 2990
TspDTI	ATGAA	6 cut(s)	31, 37, 558, 1337, 1428, 1626
TspGWI	ACGGA	4 cut(s)	299, 513, 2271, 2972
TspRI	CASTG	4 cut(s)	1084, 2245, 2916, 2999
Van91I	CCANNNNNTGG	2 cut(s)	1016, 2240
VneI	GTGCAC	1 cut(s)	1710
VpaK11BI	GGWCC	6 cut(s)	917, 931, 1007, 1020, 1361, 1864
XapI	RAATTY	3 cut(s)	1966, 2710, 2866
XceI	RCATGY	2 cut(s)	1693, 1990
XmiI	GTMKAC	2 cut(s)	605, 2178
XspI	CTAG	8 cut(s)	122, 140, 1227, 1455, 1905, 2597, 2988, 3090
ZraI	GACGTC	1 cut(s)	379
ZrmI	AGTACT	2 cut(s)	1117, 2093
Zsp2I	ATGCAT	2 cut(s)	865, 2356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.