Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

14524576 .. 14529998

Gene Structure 5423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1183900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGACCGCTCCTCCTCGCGCCGCTACGTCATCACTCTCTTTTCTCTTCGTCGTCATCGCCGTTTCCATTTCCATCTCCATCTCTCTCCTCCGCTTCAAACCCCAAAATTCCCCAACCCTAAACGTAAACCTTCCCCCAAATTCGTCGCGTCCCGAGACCAACCGCCAAAACTACGTCGTTCGATTCGTGGAATACAGGAGGGCGGAGGAGCTCCGAGAGTATTTGAAATCGAGCGTGCGATCCGATGGGTGGGATTGGATCGAGAGGAACAACCCGGCGAAGAAGTACCCGACGGATTTCGGGTTGGTTTGGATTGAAGAATCGTCGAGAGAGACGGTGGTTTCAGAAATTGGGAGCTTGGGAATGGTGAAGGATGTGAATGCCGATGTGAGGTATGGGAGGGGTTTACTTGCTGAGGGAAAGAGGGACAAAGTTGGGGCTTTTGTCGATGGGGAGAAGCGGCCGGGGAAGATTTTGACGGCGATGTCGTTCAGTGAAGCCGGCGGCGATGCATTTTACTCGGCCGCAAGTAATTCTTCGGTTAGCTGGAGAAGGCAGCTCATGTCTCAGAGATCTCAAGTTACTTCAATGTTTGGGGCGGAGTCCCTTTGGGAAAAAGGATATGCTGGTGCCAAAGTTAAAATGGCCATCTTTGACACTGGAATTCGAGCAGATCACCCGCATTTTCGTAATATCAAGGAGCGCACAAACTGGACAAATGAAGATACTTTGAATGACAACCTTGGGCATGGGACATTTGTTGCTGGTGTTATTGCTGGTGTAGATGCTGAGTGTCTTGGTTTTGCTCCTGACACAGAGATTTATGCTTTTCGTGTGTTTACAGATGCACAGGTATCATATACATCATGGTTCCTTGACGCATTCAACTATGCAATTGCGACCAATATGGATGTGCTAAATCTGAGTATAGGTGGACCTGATTACTTGGACCTCCCATTTGTAGAGAAGGTTTGGGAAATAACGGCAAACAACATAATTATGGTTTCAGCAATTGGAAATGACGGTCCGCTTTACGGAACTCTAAACAATCCAGCAGACCAAAGTGATGTCATTGGCGTTGGTGGCATTGACTACAGTGATCACATTGCTTCTTTCTCGTCACGTGGCATGAGTACATGGGAGATGCCTCATGGTTATGGTCGGCTGAAGCCTGATGTTGTTGCATATGGACGGGACATCATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCAGGCACAAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTATGTCTTCTTGTCAGTGTTATCCCTGTGAGCAATAGGAAGGATATATTAAATCCAGCAAGCATGAAACAAGCACTGGTTGAGGGTGCTGCGAAGCTTTCTGGTCCGAATATGTATGAGCAGGGTGCAGGCAGGGTTGATCTGTTAGAATCATACGAAATCCTCAAGAGCTACCAACCTCGTGCCAGCATCTTCCCTAGCACTCTCGATTATACAGATTGCCCTTACTCTTGGCCCTTCTGTCGTCAGCCCCTTTATGCAGGTGCCATGCCTGTTATCTTTAATGCTACCATTCTAAATGGAATGGGTGTAATAGGCTACGTTGAAAGTCCACCCACATGGCATCCTTCGGATGATGTAGGAAATCTTTTAAGCATTCACTTTACTTATTCTGAAGTTATTTGGCCTTGGACTGGTTATTTAGCTCTTCACATGCAAATTAAGGAAGAAGGTGCACAATTTTCTGGAGAGATTGGAGGCAATGTTACTCTTAGGGTGTACAGTCCTCCAGCTCTAGGAGAGACGGATATTCGGATTAGTACTTGTGTGCTTCAGTTGAAATTAAATATTGTCCCAACTCCGCCAAGATCGAAACGAGTTTTGTGGGATCAGTTTCACAGCATTAAATACCCTCCAGGTTATATCCCCAGAGATTCTTTGGATGTTCGTAATGACATTCTTGACTGGCATGGGGATCACCTGCATACGAATTTTCACATTATGTTCAACATGTTACGAGACGCTGGGTATTATGTCGAAACACTCGGTTCGCCATTTACTTGCTTTGATGCTCTCCGTTATGGGACACTTCTTCTTGTAGATCTTGAAGAAGAGTACTTTCGGGAGGAGATTGAAAAGCTGCAAGATGATGTACTTAATTCTGGACTGGGATTAGTGGTGTTTTCTGAATGGTATAATGTGGACACAATGGTGAAGATGAGATTCTTTGATGATAACACACGGAGCTGGTGGACTCCAGTCACTGGTGGCGCAAATGTTCCAGCATTGAATGACCTTTTAGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATTAATGGTGAGCATAGTCGATATGCATCTGGAACGAATATAGTGAGGTTTCCACGAGGTGGTTATGTGCACAAGTTCCCTTTCATGGATAGCTCTGAGAGTGGGGCCACTCAAAATGTACTTCGGACTCCTGAGATGACCACGGCAGATTCTCCCATTCTTGGTCTTGTTGAGGTGGGTGAAGGTCGTGTTGTAGTCTATGGAGACTCTAACTGTTTGGACAGCAGCCATATGGTGACAAATTGTTATTGGCTCTTGAGGAAAATATTAGATTTCACCGGTGGAAACATCAAAGACCCCGTGCTTTTCTCAAACTCGGTTAAACAAAGTTCACCTTTACATATGGAAGACAACCAATTACCGTCTCGTAGAACTGATGTGAATTTCTCTACATATTCTTCTGTTGTGGGAAAGGAATTGATGTGTGGGAATGACTCCGTGTTTGAAATCTGGGGGACTAAAGGTTACAGTTCACAGGTCAGGGGAACAAACAGAAGACTACCGGGTTATTCCGTGGTTGACTTGGATAGAGGTTTAAATTCTAAAGCCGATACTTCCAATTCAAAACGTACCAAATTGAGTGTGGAAAGTAGAAGTGATCCTTTGGGATACTTGGGCCTGTTCTATGGGGACGAGCTTGACATGCCTGTGCTAGTTCCTACTCATTGGCTTGTTCCTGCAATCATTCTTGGAACTGGACTTTTGGTATTTTTGAGCTTTTGGAGAATCCGACAGAAACGACGGCGGCGAAGGAAAGGATCTGGTTCCGGTCGGTATTCTAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

1045

Amino Acids

115.98

Weight (kDa)

5.8

Isoelectric Point (pI)

43.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	55 - 128	2.2e-14	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	210 - 462	4.3e-41	Subtilase family
MBTPS1_3rd	PF23094	490 - 618	2.2e-53	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	619 - 890	2.7e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1526, 1983
AasI	GACNNNNNNGTC	1 cut(s)	484
Acc36I	ACCTGC	2 cut(s)	1526, 1983
AccB1I	GGYRCC	2 cut(s)	629, 1538
AccB7I	CCANNNNNTGG	2 cut(s)	2258, 2405
AccBSI	CCGCTC	1 cut(s)	8
AccII	CGCG	2 cut(s)	18, 148
AclWI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1890, 1977, 2939, 3112
AcoI	YGGCCR	3 cut(s)	461, 522, 645
AcsI	RAATTY	6 cut(s)	106, 139, 663, 1984, 2728, 2884
AcuI	CTGAAG	3 cut(s)	1187, 1689, 1811
AcvI	CACGTG	1 cut(s)	1124
AdeI	CACNNNGTG	1 cut(s)	2405
AfaI	GTAC	9 cut(s)	287, 1135, 1219, 1775, 1816, 2111, 2148, 2466, 2917
AfiI	CCNNNNNNNGG	9 cut(s)	249, 390, 1034, 1688, 1790, 2258, 2405, 2431, 2507
AflIII	ACRYGT	1 cut(s)	2004
AgeI	ACCGGT	1 cut(s)	2624
AjnI	CCWGG	1 cut(s)	1909
AleI	CACNNNNGTG	2 cut(s)	1247, 2204
Alw21I	GWGCWC	3 cut(s)	213, 1732, 2418
Alw26I	GTCTC	7 cut(s)	149, 326, 570, 1790, 2007, 2544, 2715
Alw44I	GTGCAC	2 cut(s)	1728, 2414
AlwI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1890, 1977, 2939, 3112
AlwNI	CAGNNNCTG	1 cut(s)	2258
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	7 cut(s)	461, 522, 645, 1508, 1679, 2451, 2962
ApaLI	GTGCAC	2 cut(s)	1728, 2414
ApeKI	GCWGC	4 cut(s)	556, 1364, 2134, 2571
ApoI	RAATTY	6 cut(s)	106, 139, 663, 1984, 2728, 2884
ArsI	GACNNNNNNTTYG	4 cut(s)	941, 973, 2771, 2803
AseI	ATTAAT	1 cut(s)	2349
AsiGI	ACCGGT	1 cut(s)	2624
AspLEI	GCGC	3 cut(s)	20, 705, 2267
AspS9I	GGNCC	7 cut(s)	935, 949, 1025, 1379, 1509, 2451, 2962
AsuC2I	CCSGG	3 cut(s)	275, 465, 2850
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	4 cut(s)	935, 949, 1025, 1379
BaeGI	GKGCMC	2 cut(s)	1732, 2418
BalI	TGGCCA	1 cut(s)	647
BamHI	GGATCC	1 cut(s)	1205
BanI	GGYRCC	2 cut(s)	629, 1538
BanII	GRGCYC	1 cut(s)	213
BauI	CACGAG	2 cut(s)	1455, 2400
BbrPI	CACGTG	1 cut(s)	1124
BbsI	GAAGAC	3 cut(s)	1274, 2700, 2848
Bbv12I	GWGCWC	3 cut(s)	213, 1732, 2418
BbvCI	CCTCAGC	1 cut(s)	414
BbvI	GCAGC	4 cut(s)	568, 1351, 2121, 2583
BccI	CCATC	5 cut(s)	80, 86, 239, 443, 656
BceAI	ACGGC	5 cut(s)	44, 495, 999, 2505, 3104
BcgI	CGANNNNNNTGC	2 cut(s)	501, 535
BciT130I	CCWGG	1 cut(s)	1911
BciVI	GTATCC	1 cut(s)	2948
BclI	TGATCA	1 cut(s)	1099
BcnI	CCSGG	3 cut(s)	275, 465, 2850
BcoDI	GTCTC	7 cut(s)	149, 326, 570, 1790, 2007, 2544, 2715
BfaI	CTAG	3 cut(s)	1473, 1790, 2999
BfmI	CTRYAG	1 cut(s)	1093
BfuAI	ACCTGC	2 cut(s)	1526, 1983
BfuI	GTATCC	1 cut(s)	2948
BglII	AGATCT	2 cut(s)	572, 2095
BisI	GCNGC	9 cut(s)	21, 461, 505, 525, 557, 1365, 2135, 2572, 3092
BlsI	GCNGC	9 cut(s)	22, 462, 506, 526, 558, 1366, 2136, 2573, 3093
BmcAI	AGTACT	2 cut(s)	1816, 2111
Bme1390I	CCNGG	4 cut(s)	275, 465, 1911, 2850
Bme18I	GGWCC	4 cut(s)	935, 949, 1025, 1379
BmeT110I	CYCGRG	1 cut(s)	152
BmgT120I	GGNCC	7 cut(s)	935, 949, 1025, 1379, 1509, 2451, 2962
BmiI	GGNNCC	6 cut(s)	631, 872, 1207, 1540, 2452, 3112
BmrFI	CCNGG	4 cut(s)	275, 465, 1911, 2850
BmrI	ACTGGG	1 cut(s)	2171
BmsI	GCATC	8 cut(s)	499, 775, 835, 1134, 1473, 1627, 2053, 2381
BmuI	ACTGGG	1 cut(s)	2171
BoxI	GACNNNNGTC	1 cut(s)	2252
BpiI	GAAGAC	3 cut(s)	1274, 2700, 2848
BpmI	CTGGAG	5 cut(s)	568, 1761, 1767, 1893, 2235
Bpu10I	CCTNAGC	1 cut(s)	414
BpuEI	CTTGAG	3 cut(s)	561, 1424, 2623
BpuMI	CCSGG	3 cut(s)	275, 465, 2850
BsaAI	YACGTR	1 cut(s)	1124
BsaBI	GATNNNNATC	1 cut(s)	2371
BsaI	GGTCTC	1 cut(s)	149
BsaJI	CCNNGG	5 cut(s)	464, 742, 1682, 2487, 2859
BsaWI	WCCGGW	2 cut(s)	2624, 3113
BsaXI	ACNNNNNCTCC	3 cut(s)	25, 1743, 1773
Bsc4I	CCNNNNNNNGG	9 cut(s)	249, 390, 1034, 1688, 1790, 2258, 2405, 2431, 2507
Bse118I	RCCGGY	2 cut(s)	500, 2624
Bse1I	ACTGG	9 cut(s)	664, 716, 1356, 1693, 1964, 2166, 2252, 2263, 3046
Bse3DI	GCAATG	2 cut(s)	1104, 1762
Bse8I	GATNNNNATC	1 cut(s)	2371
BseBI	CCWGG	1 cut(s)	1911
BseDI	CCNNGG	5 cut(s)	464, 742, 1682, 2487, 2859
BseGI	GGATG	5 cut(s)	379, 916, 1618, 1633, 1942
BseJI	GATNNNNATC	1 cut(s)	2371
BseLI	CCNNNNNNNGG	9 cut(s)	249, 390, 1034, 1688, 1790, 2258, 2405, 2431, 2507
BseMI	GCAATG	2 cut(s)	1104, 1762
BseMII	CTCAG	6 cut(s)	405, 581, 780, 914, 2433, 2469
BseNI	ACTGG	9 cut(s)	664, 716, 1356, 1693, 1964, 2166, 2252, 2263, 3046
BseRI	GAGGAG	3 cut(s)	77, 221, 2135
BseSI	GKGCMC	2 cut(s)	1732, 2418
BseX3I	CGGCCG	2 cut(s)	461, 522
BseXI	GCAGC	4 cut(s)	568, 1351, 2121, 2583
BseYI	CCCAGC	1 cut(s)	2018
BsgI	GTGCAG	1 cut(s)	1422
Bsh1236I	CGCG	2 cut(s)	18, 148
Bsh1285I	CGRYCG	3 cut(s)	464, 525, 3118
BshFI	GGCC	7 cut(s)	463, 524, 647, 1510, 1681, 2453, 2964
BshNI	GGYRCC	2 cut(s)	629, 1538
BshTI	ACCGGT	1 cut(s)	2624
BsiEI	CGRYCG	3 cut(s)	464, 525, 3118
BsiHKAI	GWGCWC	3 cut(s)	213, 1732, 2418
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	6 cut(s)	275, 464, 501, 2625, 2849, 3114
BslFI	GGGAC	9 cut(s)	135, 440, 589, 766, 1208, 1832, 2092, 2815, 2990
BslI	CCNNNNNNNGG	9 cut(s)	249, 390, 1034, 1688, 1790, 2258, 2405, 2431, 2507
BsmAI	GTCTC	7 cut(s)	149, 326, 570, 1790, 2007, 2544, 2715
BsmBI	CGTCTC	4 cut(s)	326, 1790, 2007, 2715
BsmFI	GGGAC	9 cut(s)	135, 440, 589, 766, 1208, 1832, 2092, 2815, 2990
BsmI	GAATGC	3 cut(s)	385, 881, 1650
BsnI	GGCC	7 cut(s)	463, 524, 647, 1510, 1681, 2453, 2964
Bso31I	GGTCTC	1 cut(s)	149
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	3 cut(s)	213, 1732, 2418
Bsp1407I	TGTACA	1 cut(s)	1773
BspANI	GGCC	7 cut(s)	463, 524, 647, 1510, 1681, 2453, 2964
BspCNI	CTCAG	6 cut(s)	406, 580, 781, 915, 2434, 2470
BspFNI	CGCG	2 cut(s)	18, 148
BspLI	GGNNCC	6 cut(s)	631, 872, 1207, 1540, 2452, 3112
BspMI	ACCTGC	2 cut(s)	1526, 1983
BspPI	GGATC	8 cut(s)	234, 266, 1200, 1213, 1890, 1977, 2939, 3112
BspQI	GCTCTTC	1 cut(s)	1707
BspT107I	GGYRCC	2 cut(s)	629, 1538
BspTNI	GGTCTC	1 cut(s)	149
BsrBI	CCGCTC	1 cut(s)	8
BsrDI	GCAATG	2 cut(s)	1104, 1762
BsrFI	RCCGGY	2 cut(s)	500, 2624
BsrGI	TGTACA	1 cut(s)	1773
BsrI	ACTGG	9 cut(s)	664, 716, 1356, 1693, 1964, 2166, 2252, 2263, 3046
BssAI	RCCGGY	2 cut(s)	500, 2624
BssECI	CCNNGG	5 cut(s)	464, 742, 1682, 2487, 2859
BssSI	CACGAG	2 cut(s)	1455, 2400
BssT1I	CCWWGG	2 cut(s)	742, 1682
Bst2BI	CACGAG	2 cut(s)	1455, 2400
Bst2UI	CCWGG	1 cut(s)	1911
Bst4CI	ACNGT	7 cut(s)	337, 1025, 1097, 1778, 2561, 2709, 2816
Bst6I	CTCTTC	3 cut(s)	50, 1707, 2100
BstAUI	TGTACA	1 cut(s)	1773
BstBAI	YACGTR	1 cut(s)	1124
BstC8I	GCNNGC	5 cut(s)	236, 502, 1336, 1405, 1462
BstDEI	CTNAG	7 cut(s)	414, 567, 789, 923, 1766, 2442, 2478
BstDSI	CCRYGG	2 cut(s)	2487, 2859
BstF5I	GGATG	5 cut(s)	379, 916, 1618, 1633, 1942
BstFNI	CGCG	2 cut(s)	18, 148
BstHHI	GCGC	3 cut(s)	20, 705, 2267
BstMAI	GTCTC	7 cut(s)	149, 326, 570, 1790, 2007, 2544, 2715
BstMCI	CGRYCG	3 cut(s)	464, 525, 3118
BstMWI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 2989
BstNI	CCWGG	1 cut(s)	1911
BstNSI	RCATGY	3 cut(s)	1711, 2008, 2992
BstPAI	GACNNNNGTC	1 cut(s)	2252
BstSCI	CCNGG	4 cut(s)	273, 463, 1909, 2848
BstSFI	CTRYAG	1 cut(s)	1093
BstSLI	GKGCMC	2 cut(s)	1732, 2418
BstUI	CGCG	2 cut(s)	18, 148
BstV1I	GCAGC	4 cut(s)	568, 1351, 2121, 2583
BstV2I	GAAGAC	3 cut(s)	1274, 2700, 2848
BstX2I	RGATCY	4 cut(s)	572, 1205, 2095, 3104
BstXI	CCANNNNNNTGG	1 cut(s)	1614
BstYI	RGATCY	4 cut(s)	572, 1205, 2095, 3104
BstZI	CGGCCG	2 cut(s)	461, 522
BsuI	GTATCC	1 cut(s)	2948
BsuRI	GGCC	7 cut(s)	463, 524, 647, 1510, 1681, 2453, 2964
BtgI	CCRYGG	2 cut(s)	2487, 2859
BtgZI	GCGATG	3 cut(s)	40, 497, 522
BtsCI	GGATG	5 cut(s)	379, 916, 1618, 1633, 1942
BtsIMutI	CAGTG	6 cut(s)	499, 657, 1102, 1297, 1349, 2256
BveI	ACCTGC	2 cut(s)	1526, 1983
Cac8I	GCNNGC	5 cut(s)	236, 502, 1336, 1405, 1462
CaiI	CAGNNNCTG	1 cut(s)	2258
CfoI	GCGC	3 cut(s)	20, 705, 2267
Cfr10I	RCCGGY	2 cut(s)	500, 2624
Cfr13I	GGNCC	7 cut(s)	935, 949, 1025, 1379, 1509, 2451, 2962
CpoI	CGGWCCG	1 cut(s)	1025
CseI	GACGC	3 cut(s)	137, 887, 2024
Csp6I	GTAC	9 cut(s)	286, 1134, 1218, 1774, 1815, 2110, 2147, 2465, 2916
CspAI	ACCGGT	1 cut(s)	2624
CspCI	CAANNNNNGTGG	2 cut(s)	1242, 1277
CspI	CGGWCCG	1 cut(s)	1025
CviQI	GTAC	9 cut(s)	286, 1134, 1218, 1774, 1815, 2110, 2147, 2465, 2916
DdeI	CTNAG	7 cut(s)	414, 567, 789, 923, 1766, 2442, 2478
DraI	TTTAAA	1 cut(s)	2883
DraIII	CACNNNGTG	1 cut(s)	2405
DrdI	GACNNNNNNGTC	1 cut(s)	484
DseDI	GACNNNNNNGTC	1 cut(s)	484
EaeI	YGGCCR	3 cut(s)	461, 522, 645
EagI	CGGCCG	2 cut(s)	461, 522
Eam1104I	CTCTTC	3 cut(s)	50, 1707, 2100
EarI	CTCTTC	3 cut(s)	50, 1707, 2100
EciI	GGCGGA	3 cut(s)	218, 614, 1845
Ecl136II	GAGCTC	1 cut(s)	211
EclXI	CGGCCG	2 cut(s)	461, 522
Eco130I	CCWWGG	2 cut(s)	742, 1682
Eco24I	GRGCYC	1 cut(s)	213
Eco31I	GGTCTC	1 cut(s)	149
Eco47I	GGWCC	4 cut(s)	935, 949, 1025, 1379
Eco52I	CGGCCG	2 cut(s)	461, 522
Eco53kI	GAGCTC	1 cut(s)	211
Eco57I	CTGAAG	3 cut(s)	1187, 1689, 1811
Eco72I	CACGTG	1 cut(s)	1124
Eco88I	CYCGRG	1 cut(s)	152
EcoICRI	GAGCTC	1 cut(s)	211
EcoRI	GAATTC	1 cut(s)	663
EcoRII	CCWGG	1 cut(s)	1909
EcoT14I	CCWWGG	2 cut(s)	742, 1682
EcoT22I	ATGCAT	2 cut(s)	514, 2374
EcoT38I	GRGCYC	1 cut(s)	213
ErhI	CCWWGG	2 cut(s)	742, 1682
Esp3I	CGTCTC	4 cut(s)	326, 1790, 2007, 2715
FalI	AAGNNNNNCTT	6 cut(s)	520, 552, 2665, 2697, 2932, 2964
FaqI	GGGAC	9 cut(s)	135, 440, 589, 766, 1208, 1832, 2092, 2815, 2990
FauI	CCCGC	1 cut(s)	687
FauNDI	CATATG	3 cut(s)	1186, 2577, 2688
FbaI	TGATCA	1 cut(s)	1099
Fnu4HI	GCNGC	9 cut(s)	21, 461, 505, 525, 557, 1365, 2135, 2572, 3092
FokI	GGATG	5 cut(s)	386, 923, 1605, 1640, 1949
FriOI	GRGCYC	1 cut(s)	213
Fsp4HI	GCNGC	9 cut(s)	21, 461, 505, 525, 557, 1365, 2135, 2572, 3092
FspBI	CTAG	3 cut(s)	1473, 1790, 2999
GlaI	GCGC	3 cut(s)	19, 704, 2266
GluI	GCNGC	9 cut(s)	21, 461, 505, 525, 557, 1365, 2135, 2572, 3092
GsaI	CCCAGC	1 cut(s)	2022
GsuI	CTGGAG	5 cut(s)	568, 1761, 1767, 1893, 2235
HaeIII	GGCC	7 cut(s)	463, 524, 647, 1510, 1681, 2453, 2964
HapII	CCGG	6 cut(s)	275, 464, 501, 2625, 2849, 3114
HgaI	GACGC	3 cut(s)	137, 887, 2024
HhaI	GCGC	3 cut(s)	20, 705, 2267
Hin6I	GCGC	3 cut(s)	18, 703, 2265
HinP1I	GCGC	3 cut(s)	18, 703, 2265
HincII	GTYRAC	1 cut(s)	2866
HindII	GTYRAC	1 cut(s)	2866
HindIII	AAGCTT	2 cut(s)	1231, 1370
HpaII	CCGG	6 cut(s)	275, 464, 501, 2625, 2849, 3114
Hpy99I	CGWCG	6 cut(s)	53, 148, 179, 295, 328, 3090
HpyCH4III	ACNGT	7 cut(s)	337, 1025, 1097, 1778, 2561, 2709, 2816
HpyCH4IV	ACGT	6 cut(s)	26, 123, 174, 1123, 1596, 2914
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 2989
HpyF3I	CTNAG	7 cut(s)	414, 567, 789, 923, 1766, 2442, 2478
HpySE526I	ACGT	6 cut(s)	26, 123, 174, 1123, 1596, 2914
HspAI	GCGC	3 cut(s)	18, 703, 2265
KroI	GCCGGC	1 cut(s)	500
KroNI	GCCGGC	1 cut(s)	502
Ksp22I	TGATCA	1 cut(s)	1099
LguI	GCTCTTC	1 cut(s)	1707
LmnI	GCTCC	8 cut(s)	13, 208, 216, 354, 700, 811, 2238, 2302
Lsp1109I	GCAGC	4 cut(s)	568, 1351, 2121, 2583
LweI	GCATC	8 cut(s)	499, 775, 835, 1134, 1473, 1627, 2053, 2381
MaeI	CTAG	3 cut(s)	1473, 1790, 2999
MaeII	ACGT	6 cut(s)	26, 123, 174, 1123, 1596, 2914
MaeIII	GTNAC	7 cut(s)	580, 1119, 1759, 2007, 2254, 2581, 2810
MbiI	CCGCTC	1 cut(s)	8
MfeI	CAATTG	2 cut(s)	894, 1011
MflI	RGATCY	4 cut(s)	572, 1205, 2095, 3104
MhlI	GDGCHC	3 cut(s)	213, 1732, 2418
MlsI	TGGCCA	1 cut(s)	647
MluNI	TGGCCA	1 cut(s)	647
MlyI	GAGTC	5 cut(s)	611, 2242, 2467, 2546, 2774
MmeI	TCCRAC	1 cut(s)	3100
Mox20I	TGGCCA	1 cut(s)	647
Mph1103I	ATGCAT	2 cut(s)	514, 2374
MroNI	GCCGGC	1 cut(s)	500
MscI	TGGCCA	1 cut(s)	647
MslI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1612, 2204, 2993
Msp20I	TGGCCA	1 cut(s)	647
MspI	CCGG	6 cut(s)	275, 464, 501, 2625, 2849, 3114
MspR9I	CCNGG	4 cut(s)	275, 465, 1911, 2850
MunI	CAATTG	2 cut(s)	894, 1011
Mva1269I	GAATGC	3 cut(s)	385, 881, 1650
MvaI	CCWGG	1 cut(s)	1911
MvnI	CGCG	2 cut(s)	18, 148
MwoI	GCNNNNNNNGC	4 cut(s)	1083, 1230, 1239, 2989
NaeI	GCCGGC	1 cut(s)	502
NciI	CCSGG	3 cut(s)	275, 465, 2850
NdeI	CATATG	3 cut(s)	1186, 2577, 2688
NgoMIV	GCCGGC	1 cut(s)	500
NlaIV	GGNNCC	6 cut(s)	631, 872, 1207, 1540, 2452, 3112
NmeAIII	GCCGAG	1 cut(s)	500
NmuCI	GTSAC	3 cut(s)	1119, 2254, 2581
NsiI	ATGCAT	2 cut(s)	514, 2374
NspI	RCATGY	3 cut(s)	1711, 2008, 2992
OliI	CACNNNNGTG	2 cut(s)	1247, 2204
PaqCI	CACCTGC	2 cut(s)	1526, 1983
PciI	ACATGT	1 cut(s)	2004
PciSI	GCTCTTC	1 cut(s)	1707
PcsI	WCGNNNNNNNCGW	4 cut(s)	57, 183, 485, 3091
PctI	GAATGC	3 cut(s)	385, 881, 1650
PdiI	GCCGGC	1 cut(s)	502
PfeI	GAWTC	8 cut(s)	183, 320, 1424, 1928, 2217, 2326, 2495, 3072
PflMI	CCANNNNNTGG	2 cut(s)	2258, 2405
PinAI	ACCGGT	1 cut(s)	2624
PkrI	GCNGC	9 cut(s)	22, 462, 506, 526, 558, 1366, 2136, 2573, 3093
PleI	GAGTC	5 cut(s)	610, 2242, 2467, 2546, 2774
PmaCI	CACGTG	1 cut(s)	1124
PmlI	CACGTG	1 cut(s)	1124
PpsI	GAGTC	5 cut(s)	610, 2242, 2467, 2546, 2774
Ppu21I	YACGTR	1 cut(s)	1124
PscI	ACATGT	1 cut(s)	2004
PshAI	GACNNNNGTC	1 cut(s)	2252
PshBI	ATTAAT	1 cut(s)	2349
Psp124BI	GAGCTC	1 cut(s)	213
Psp6I	CCWGG	1 cut(s)	1909
PspCI	CACGTG	1 cut(s)	1124
PspFI	CCCAGC	1 cut(s)	2018
PspGI	CCWGG	1 cut(s)	1909
PspN4I	GGNNCC	6 cut(s)	631, 872, 1207, 1540, 2452, 3112
PspPI	GGNCC	7 cut(s)	935, 949, 1025, 1379, 1509, 2451, 2962
PstNI	CAGNNNCTG	1 cut(s)	2258
PsuI	RGATCY	4 cut(s)	572, 1205, 2095, 3104
RsaI	GTAC	9 cut(s)	287, 1135, 1219, 1775, 1816, 2111, 2148, 2466, 2917
RsaNI	GTAC	9 cut(s)	286, 1134, 1218, 1774, 1815, 2110, 2147, 2465, 2916
RseI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1612, 2204, 2993
Rsr2I	CGGWCCG	1 cut(s)	1025
RsrII	CGGWCCG	1 cut(s)	1025
SacI	GAGCTC	1 cut(s)	213
SapI	GCTCTTC	1 cut(s)	1707
SatI	GCNGC	9 cut(s)	21, 461, 505, 525, 557, 1365, 2135, 2572, 3092
Sau96I	GGNCC	7 cut(s)	935, 949, 1025, 1379, 1509, 2451, 2962
ScaI	AGTACT	2 cut(s)	1816, 2111
SchI	GAGTC	5 cut(s)	611, 2242, 2467, 2546, 2774
ScrFI	CCNGG	4 cut(s)	275, 465, 1911, 2850
SduI	GDGCHC	3 cut(s)	213, 1732, 2418
SfaNI	GCATC	8 cut(s)	499, 775, 835, 1134, 1473, 1627, 2053, 2381
SfcI	CTRYAG	1 cut(s)	1093
SgrAI	CRCCGGYG	1 cut(s)	2624
SinI	GGWCC	4 cut(s)	935, 949, 1025, 1379
SmiMI	CAYNNNNRTG	6 cut(s)	998, 1155, 1247, 1612, 2204, 2993
SmlI	CTYRAG	3 cut(s)	576, 1439, 2602
SmoI	CTYRAG	3 cut(s)	576, 1439, 2602
SspI	AATATT	2 cut(s)	1843, 2613
SspMI	CTAG	3 cut(s)	1473, 1790, 2999
SstI	GAGCTC	1 cut(s)	213
StyD4I	CCNGG	4 cut(s)	273, 463, 1909, 2848
StyI	CCWWGG	2 cut(s)	742, 1682
TaaI	ACNGT	7 cut(s)	337, 1025, 1097, 1778, 2561, 2709, 2816
TaiI	ACGT	6 cut(s)	29, 126, 177, 1126, 1599, 2917
TatI	WGTACW	7 cut(s)	1133, 1217, 1773, 1814, 2109, 2146, 2464
TauI	GCSGC	5 cut(s)	23, 463, 507, 527, 3094
TfiI	GAWTC	8 cut(s)	183, 320, 1424, 1928, 2217, 2326, 2495, 3072
TscAI	CASTG	6 cut(s)	499, 664, 1102, 1297, 1356, 2263
TseFI	GTSAC	3 cut(s)	1119, 2254, 2581
TseI	GCWGC	4 cut(s)	556, 1364, 2134, 2571
Tsp45I	GTSAC	3 cut(s)	1119, 2254, 2581
TspDTI	ATGAA	3 cut(s)	735, 1355, 2419
TspGWI	ACGGA	8 cut(s)	308, 1050, 1814, 2060, 2251, 2289, 2773, 2848
TspRI	CASTG	6 cut(s)	499, 664, 1102, 1297, 1356, 2263
Van91I	CCANNNNNTGG	2 cut(s)	2258, 2405
VneI	GTGCAC	2 cut(s)	1728, 2414
VpaK11BI	GGWCC	4 cut(s)	935, 949, 1025, 1379
VspI	ATTAAT	1 cut(s)	2349
XapI	RAATTY	6 cut(s)	106, 139, 663, 1984, 2728, 2884
XceI	RCATGY	3 cut(s)	1711, 2008, 2992
XcmI	CCANNNNNNNNNTGG	3 cut(s)	640, 1930, 2926
XspI	CTAG	3 cut(s)	1473, 1790, 2999
ZrmI	AGTACT	2 cut(s)	1816, 2111
Zsp2I	ATGCAT	2 cut(s)	514, 2374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.