Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

4148763 .. 4153961

Gene Structure 5199 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ46553

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGACCACTCCTCTGACCGCCACGTCATCACTATCCTTCTTCTTCTTCATCATCGTCTCCATCGCCGTAGCCGTCTCCATCTCTCTCTTCCATTACAAACCCCACCGGAACCCCGCAGCCGTAGATCCTCCTCCACCGCAAAACTACGTCGTTCGATTCGTCGAGTACAAGAGGTGGGAGGATCACCGGGAGTATCTGAAATCGAGTGTGCCGGCGGAGGGATGGGACTGGATCGAGAGGAACAATCCGGCCATGGATTACCCGACGGATTTCGGGCTGGTGCGGATTGACGATGGAGCCAGGGAATTGGTGATTGGGGAGATTGGGAAATTGGGGATGGTGAAGGATGTGAATGCTGACGTCAGGTATGGGAGGGGTTTGCTTAGTGAGGAGGAGAAAGTTGGGGCCTTTGTGGATGGGGAGAAGCGGCCGGGGAAGATGTTCACGGCGATGTCGTTTAGCGAAGACGACGGAGCAGTGAGTAATGGCTCTGTTAGCTGGAAAAGGGAGCTCTTGGCTGAGAGATCTCAAATAACTTCATTATTTGGAGCGGATTTTCTTTGGACAAAAGGGTATACCGGTACGAAAGTGAAAATGGCCATATTTGATACTGGTATTCGAGCTAATCACCCGCACTTTCGAAATATTAAGGAGCGGACAAACTGGACAAATGAGGACACATTAAATGACAATCTTGGACATGGGACATTTGTTGCTGGTGTTGTTGCTGGTGTAGATGCAGAGTGTCTTGGATTTGCTCCTGATACAGAGATATATGCGTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGATGCATTCAACTATGCCATTGCGACCAATATGGATGTACTGAACTTGAGCATAGGTGGACCTGATTACTTGGACCTCCCATTTGTGGAGAAGGTCTGGGAAATAACAGCAAACAACATAATTATGGTTTCAGCAATTGGAAATGATGGACCACTTTATGGGACTCTAAATAATCCAGCAGACCAAAGTGATGTCATTGGTGTTGGTGGCATCGACTACAGTGATCACATTGCTTCTTTTTCCTCACGAGGCATGAGTACTTGGGAGATGCCGCACGGTTACGGTCGTGTTAAGCCAGATGTCGTTGCATATGGACGGGAAATTATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCTGGCACTAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTTTGTCTGCTTGTGAGTGTTATCCCTGAAAGCAGTCGGAAGGATACTTTAAATCCAGCAAGCATGAAACAAGCATTGGTTGAGGGTGCTGCAAAACTCGGTGGTCCAAATATGTATGAGCAGGGTGCAGGGAGGGTTGATCTGTTAGAATCATATGAAATCCTGAAGAATTACCAACCTCGTGCCAGCATCTTCCCTAGTATTCTTGATTATACAGACTGCCCATACTCTTGGCCCTTCTGTCGTCAGCCACTTTATGCTGGTGCCATGCCTGTTATCTTCAATGCTACCATTCTAAATGGAATGGGTGTAATTGGCTATGTTGAAAGTCCACCAACTTGGCATCCTTCAGATGAAGTAGGGAATCTTCTAAGTATTCACTTTACATATTCTAATGTTATCTGGCCTTGGACTGGTTATTTAGCGCTTCACATGCAAATTAAGGACGAAGGTGCACAATTTTCAGGAGAGATTAATGGCAATGTTACTCTTAGGGTATACAGTCCTCCATCTCCGGGAGAAAAGAATCTTCGGATTAGCACTTGTGTGCTTCAGTTAAGATTGAAGATTGTTCCAACTCCACCAAGATCAAAACGAGTTTTGTGGGACCAGTTTCACAGTATTAAATACCCTCCAGGTTACATCCCTAGAGACTCTTTGGATGTTCGCAATGACATTCTTGACTGGCATGGAGATCACCTGCATACAAATTTTCACATTATGTTCAACATGTTACGAGATGCTGGGTATTATGTAGAAACACTTGGTTCGCCTCTTACTTGCTTTGATGCTCTTCGATATGGGACACTTCTTCTGGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAGAAGCTGCGAGATGATGTTCTTAATTCTGGACTGGGATTGGTTGTGTTTTCTGAGTGGTATAATGTAGACACGATGGTGAAGATGAGATTCTTTGATGATAACACACGTAGCTGGTGGACTCCAGTCACTGGAGGTGCAAATGTTCCAGCGTTGAACGACCTGCTGGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATCAATGGTGAGCAAAGTCGGTATGCATCTGGAACGGATATTGTGAGGTTTCCGAAAGGGGGTTATGTACACAAGTTCCCTTTCCTGGATAGCTCTGAAAGTGGGGCCACTCAGAATGTACTTCGGGATCCTGAGGTGACAATGGCAGATTCTCCCATTCTTGGTCTTGTACAGGTGGGTGAAGGTCGTGTTGTAGTGTATGGAGATTCTAACTGTTTGGACAGCAGTCATATGGTAACTAATTGTTATTGGCTCTTGAGAAAAATGTTAGATTTCACCGGTGGAAACATCAGAGATCCTGTGCTTTTTTCAAGCTCGGTCAAACAAAGTTCACCTTTATATATGGAAGATAACCAATTACCTTCTCGTAGAACAGATGTGAATTTTTCTACATATTCCGCTGTAGTGGGGAAGGAATTGATCTGTGGGAGTGACTCTGTATTTGAAATATGGGGGACTAAAGGTTACAGTTTACAGGTCAGGGGAACAAACAGAAGATTACCAGGCTCTTCTGTGTCTAATCCAGGTAGGGGTTTGAATTCTACTGTCGAGGCTTCCCATTTGGAGCATTCCAAGTTGACACTGGGAAATAGAAGTGATCCTTCGGGATATAGGGATGAGCTTGATATGCCTGTGTTAGTTGCTAGTCACTGGCTTGTTCCTGCAATAATTGCCGTAACTGGTTTGGTGGTATTTTTGAGCTTCTGGAGAATTAGACAGAAGCGACGACGACGAAGAAAAGGATCTAGCTCTGGTCGATATTCTAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

1023

Amino Acids

113.69

Weight (kDa)

5.55

Isoelectric Point (pI)

41.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	47 - 119	4.6e-13	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	194 - 446	8.4e-42	Subtilase family
MBTPS1_3rd	PF23094	474 - 602	2.7e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	603 - 874	2.2e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1935
AasI	GACNNNNNNGTC	1 cut(s)	22
AatII	GACGTC	1 cut(s)	363
Acc36I	ACCTGC	2 cut(s)	1935, 2250
AccB1I	GGYRCC	1 cut(s)	1490
AccB7I	CCANNNNNTGG	2 cut(s)	986, 2210
AccBSI	CCGCTC	2 cut(s)	551, 655
AccI	GTMKAC	3 cut(s)	575, 1725, 2148
AcoI	YGGCCR	3 cut(s)	249, 428, 597
AcsI	RAATTY	3 cut(s)	1936, 2680, 2836
AcuI	CTGAAG	3 cut(s)	1412, 1560, 1763
AcyI	GRCGYC	1 cut(s)	360
AfaI	GTAC	9 cut(s)	167, 583, 867, 1087, 1171, 2063, 2367, 2418, 2469
AfeI	AGCGCT	1 cut(s)	1653
AflIII	ACRYGT	2 cut(s)	1956, 2186
AgeI	ACCGGT	2 cut(s)	578, 2576
AhlI	ACTAGT	1 cut(s)	1196
AjiI	CACGTC	1 cut(s)	24
AjnI	CCWGG	5 cut(s)	299, 1861, 2382, 2800, 2821
AjuI	GAANNNNNNNTTGG	4 cut(s)	297, 329, 1286, 1318
AleI	CACNNNNGTG	3 cut(s)	1199, 1772, 2156
Alw21I	GWGCWC	2 cut(s)	513, 1684
Alw26I	GTCTC	3 cut(s)	61, 79, 1872
Alw44I	GTGCAC	1 cut(s)	1680
AlwNI	CAGNNNCTG	1 cut(s)	2210
Aor51HI	AGCGCT	1 cut(s)	1653
AoxI	GGCC	7 cut(s)	249, 405, 428, 597, 1460, 1631, 2403
ApaLI	GTGCAC	1 cut(s)	1680
ApeKI	GCWGC	3 cut(s)	116, 1316, 2086
ApoI	RAATTY	3 cut(s)	1936, 2680, 2836
ArsI	GACNNNNNNTTYG	4 cut(s)	893, 925, 2723, 2755
AseI	ATTAAT	1 cut(s)	1701
AsiGI	ACCGGT	2 cut(s)	578, 2576
Asp700I	GAANNNNTTC	1 cut(s)	440
AspLEI	GCGC	1 cut(s)	1654
AspS9I	GGNCC	8 cut(s)	405, 887, 901, 977, 1331, 1461, 1834, 2403
AsuC2I	CCSGG	3 cut(s)	188, 432, 1743
AsuII	TTCGAA	1 cut(s)	640
AvaII	GGWCC	5 cut(s)	887, 901, 977, 1331, 1834
AxyI	CCTNAGG	1 cut(s)	2430
BaeGI	GKGCMC	1 cut(s)	1684
BaeI	ACNNNNGTAYC	2 cut(s)	573, 606
BalI	TGGCCA	1 cut(s)	599
BamHI	GGATCC	2 cut(s)	1157, 2425
BanI	GGYRCC	1 cut(s)	1490
BanII	GRGCYC	1 cut(s)	513
BarI	GAAGNNNNNNTAC	2 cut(s)	1578, 1610
BauI	CACGAG	2 cut(s)	1074, 1407
BbsI	GAAGAC	1 cut(s)	471
Bbv12I	GWGCWC	2 cut(s)	513, 1684
BbvI	GCAGC	3 cut(s)	128, 1303, 2073
BccI	CCATC	9 cut(s)	68, 86, 216, 287, 331, 410, 968, 1744, 2149
BceAI	ACGGC	5 cut(s)	50, 56, 104, 462, 2957
BciT130I	CCWGG	5 cut(s)	301, 1863, 2384, 2802, 2823
BciVI	GTATCC	1 cut(s)	1264
BclI	TGATCA	1 cut(s)	1051
BcnI	CCSGG	3 cut(s)	188, 432, 1743
BcoDI	GTCTC	3 cut(s)	61, 79, 1872
BcuI	ACTAGT	1 cut(s)	1196
BfaI	CTAG	5 cut(s)	1197, 1425, 1875, 2943, 3045
BfmI	CTRYAG	2 cut(s)	1045, 2700
BfoI	RGCGCY	1 cut(s)	1655
BfuAI	ACCTGC	2 cut(s)	1935, 2250
BfuI	GTATCC	1 cut(s)	1264
BglII	AGATCT	2 cut(s)	524, 2047
BisI	GCNGC	5 cut(s)	117, 428, 1100, 1317, 2087
BlsI	GCNGC	5 cut(s)	118, 429, 1101, 1318, 2088
BmcAI	AGTACT	2 cut(s)	1087, 2063
Bme1390I	CCNGG	8 cut(s)	188, 301, 432, 1743, 1863, 2384, 2802, 2823
Bme18I	GGWCC	5 cut(s)	887, 901, 977, 1331, 1834
BmgBI	CACGTC	1 cut(s)	24
BmgT120I	GGNCC	8 cut(s)	405, 887, 901, 977, 1331, 1461, 1834, 2403
BmrFI	CCNGG	8 cut(s)	188, 301, 432, 1743, 1863, 2384, 2802, 2823
BmrI	ACTGGG	2 cut(s)	2123, 2891
BmuI	ACTGGG	2 cut(s)	2123, 2891
BoxI	GACNNNNGTC	2 cut(s)	2204, 2523
BpiI	GAAGAC	1 cut(s)	471
BpmI	CTGGAG	4 cut(s)	1845, 2187, 2232, 3025
Bpu14I	TTCGAA	1 cut(s)	640
BpuEI	CTTGAG	2 cut(s)	895, 2575
BpuMI	CCSGG	3 cut(s)	188, 432, 1743
BsaAI	YACGTR	1 cut(s)	2189
BsaHI	GRCGYC	1 cut(s)	360
BsaJI	CCNNGG	4 cut(s)	252, 300, 431, 1634
BsaWI	WCCGGW	3 cut(s)	105, 578, 2576
Bse118I	RCCGGY	3 cut(s)	211, 578, 2576
Bse21I	CCTNAGG	1 cut(s)	2430
Bse3DI	GCAATG	4 cut(s)	846, 1056, 1714, 1903
BseBI	CCWGG	5 cut(s)	301, 1863, 2384, 2802, 2823
BseDI	CCNNGG	4 cut(s)	252, 300, 431, 1634
BseGI	GGATG	9 cut(s)	227, 342, 352, 421, 868, 1570, 1869, 1894, 2920
BseMI	GCAATG	4 cut(s)	846, 1056, 1714, 1903
BseMII	CTCAG	4 cut(s)	510, 2124, 2421, 2423
BseRI	GAGGAG	4 cut(s)	120, 404, 407, 2087
BseSI	GKGCMC	1 cut(s)	1684
BseX3I	CGGCCG	1 cut(s)	428
BseXI	GCAGC	3 cut(s)	128, 1303, 2073
BseYI	CCCAGC	1 cut(s)	1970
BsgI	GTGCAG	1 cut(s)	1374
Bsh1285I	CGRYCG	2 cut(s)	431, 1114
BshFI	GGCC	7 cut(s)	251, 407, 430, 599, 1462, 1633, 2405
BshNI	GGYRCC	1 cut(s)	1490
BshTI	ACCGGT	2 cut(s)	578, 2576
BsiEI	CGRYCG	2 cut(s)	431, 1114
BsiHKAI	GWGCWC	2 cut(s)	513, 1684
BsiSI	CCGG	8 cut(s)	106, 187, 212, 248, 431, 579, 1742, 2577
BslFI	GGGAC	6 cut(s)	239, 718, 1003, 1847, 2044, 2767
BsmAI	GTCTC	3 cut(s)	61, 79, 1872
BsmBI	CGTCTC	2 cut(s)	61, 79
BsmFI	GGGAC	6 cut(s)	239, 718, 1003, 1847, 2044, 2767
BsmI	GAATGC	3 cut(s)	358, 833, 2866
BsnI	GGCC	7 cut(s)	251, 407, 430, 599, 1462, 1633, 2405
Bsp119I	TTCGAA	1 cut(s)	640
Bsp1286I	GDGCHC	2 cut(s)	513, 1684
Bsp1407I	TGTACA	2 cut(s)	2365, 2467
Bsp19I	CCATGG	1 cut(s)	252
BspANI	GGCC	7 cut(s)	251, 407, 430, 599, 1462, 1633, 2405
BspCNI	CTCAG	4 cut(s)	511, 2125, 2422, 2422
BspMI	ACCTGC	2 cut(s)	1935, 2250
BspQI	GCTCTTC	2 cut(s)	2025, 2812
BspT104I	TTCGAA	1 cut(s)	640
BspT107I	GGYRCC	1 cut(s)	1490
BsrBI	CCGCTC	2 cut(s)	551, 655
BsrDI	GCAATG	4 cut(s)	846, 1056, 1714, 1903
BsrFI	RCCGGY	3 cut(s)	211, 578, 2576
BsrGI	TGTACA	2 cut(s)	2365, 2467
BssAI	RCCGGY	3 cut(s)	211, 578, 2576
BssECI	CCNNGG	4 cut(s)	252, 300, 431, 1634
BssNAI	GTATAC	2 cut(s)	576, 1726
BssNI	GRCGYC	1 cut(s)	360
BssSI	CACGAG	2 cut(s)	1074, 1407
BssT1I	CCWWGG	2 cut(s)	252, 1634
Bst1107I	GTATAC	2 cut(s)	576, 1726
Bst2BI	CACGAG	2 cut(s)	1074, 1407
Bst2UI	CCWGG	5 cut(s)	301, 1863, 2384, 2802, 2823
Bst4CI	ACNGT	8 cut(s)	1049, 1106, 1112, 1730, 1847, 2513, 2768, 2845
Bst6I	CTCTTC	4 cut(s)	92, 2025, 2052, 2812
BstACI	GRCGYC	1 cut(s)	360
BstAPI	GCANNNNNTGC	1 cut(s)	2969
BstAUI	TGTACA	2 cut(s)	2365, 2467
BstBAI	YACGTR	1 cut(s)	2189
BstBI	TTCGAA	1 cut(s)	640
BstC8I	GCNNGC	4 cut(s)	213, 1288, 1414, 2247
BstDEI	CTNAG	7 cut(s)	383, 519, 1598, 1718, 2133, 2409, 2430
BstDSI	CCRYGG	1 cut(s)	252
BstF5I	GGATG	9 cut(s)	227, 342, 352, 421, 868, 1570, 1869, 1894, 2920
BstH2I	RGCGCY	1 cut(s)	1655
BstHHI	GCGC	1 cut(s)	1654
BstMAI	GTCTC	3 cut(s)	61, 79, 1872
BstMCI	CGRYCG	2 cut(s)	431, 1114
BstMWI	GCNNNNNNNGC	6 cut(s)	495, 1182, 1191, 1660, 2926, 2969
BstNI	CCWGG	5 cut(s)	301, 1863, 2384, 2802, 2823
BstNSI	RCATGY	2 cut(s)	1663, 1960
BstPAI	GACNNNNGTC	2 cut(s)	2204, 2523
BstSCI	CCNGG	8 cut(s)	186, 299, 430, 1741, 1861, 2382, 2800, 2821
BstSFI	CTRYAG	2 cut(s)	1045, 2700
BstSLI	GKGCMC	1 cut(s)	1684
BstV1I	GCAGC	3 cut(s)	128, 1303, 2073
BstV2I	GAAGAC	1 cut(s)	471
BstX2I	RGATCY	7 cut(s)	124, 524, 1157, 2047, 2425, 2593, 3041
BstXI	CCANNNNNNTGG	3 cut(s)	307, 1458, 1566
BstYI	RGATCY	7 cut(s)	124, 524, 1157, 2047, 2425, 2593, 3041
BstZ17I	GTATAC	2 cut(s)	576, 1726
BstZI	CGGCCG	1 cut(s)	428
Bsu36I	CCTNAGG	1 cut(s)	2430
BsuI	GTATCC	1 cut(s)	1264
BsuRI	GGCC	7 cut(s)	251, 407, 430, 599, 1462, 1633, 2405
BtgI	CCRYGG	1 cut(s)	252
BtgZI	GCGATG	2 cut(s)	46, 464
BtrI	CACGTC	1 cut(s)	24
BtsCI	GGATG	9 cut(s)	227, 342, 352, 421, 868, 1570, 1869, 1894, 2920
BtsI	GCAGTG	1 cut(s)	483
BtsIMutI	CAGTG	5 cut(s)	483, 1054, 2208, 2879, 2947
BveI	ACCTGC	2 cut(s)	1935, 2250
Cac8I	GCNNGC	4 cut(s)	213, 1288, 1414, 2247
CaiI	CAGNNNCTG	1 cut(s)	2210
CfoI	GCGC	1 cut(s)	1654
Cfr10I	RCCGGY	3 cut(s)	211, 578, 2576
Cfr13I	GGNCC	8 cut(s)	405, 887, 901, 977, 1331, 1461, 1834, 2403
Csp6I	GTAC	9 cut(s)	166, 582, 866, 1086, 1170, 2062, 2366, 2417, 2468
CspAI	ACCGGT	2 cut(s)	578, 2576
CspCI	CAANNNNNGTGG	2 cut(s)	1194, 1229
CviAII	CATG	9 cut(s)	253, 701, 819, 1081, 1291, 1495, 1660, 1916, 1957
CviQI	GTAC	9 cut(s)	166, 582, 866, 1086, 1170, 2062, 2366, 2417, 2468
DdeI	CTNAG	7 cut(s)	383, 519, 1598, 1718, 2133, 2409, 2430
DraI	TTTAAA	1 cut(s)	1278
DrdI	GACNNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	3 cut(s)	249, 428, 597
EagI	CGGCCG	1 cut(s)	428
Eam1104I	CTCTTC	4 cut(s)	92, 2025, 2052, 2812
EarI	CTCTTC	4 cut(s)	92, 2025, 2052, 2812
EciI	GGCGGA	1 cut(s)	230
Ecl136II	GAGCTC	1 cut(s)	511
EclXI	CGGCCG	1 cut(s)	428
Eco130I	CCWWGG	2 cut(s)	252, 1634
Eco24I	GRGCYC	1 cut(s)	513
Eco47I	GGWCC	5 cut(s)	887, 901, 977, 1331, 1834
Eco47III	AGCGCT	1 cut(s)	1653
Eco52I	CGGCCG	1 cut(s)	428
Eco53kI	GAGCTC	1 cut(s)	511
Eco57I	CTGAAG	3 cut(s)	1412, 1560, 1763
Eco81I	CCTNAGG	1 cut(s)	2430
EcoICRI	GAGCTC	1 cut(s)	511
EcoO109I	RGGNCCY	1 cut(s)	405
EcoRI	GAATTC	1 cut(s)	2836
EcoRII	CCWGG	5 cut(s)	299, 1861, 2382, 2800, 2821
EcoT14I	CCWWGG	2 cut(s)	252, 1634
EcoT22I	ATGCAT	2 cut(s)	835, 2326
EcoT38I	GRGCYC	1 cut(s)	513
ErhI	CCWWGG	2 cut(s)	252, 1634
Esp3I	CGTCTC	2 cut(s)	61, 79
FaeI	CATG	9 cut(s)	256, 704, 822, 1084, 1294, 1498, 1663, 1919, 1960
FalI	AAGNNNNNCTT	4 cut(s)	2617, 2649, 2884, 2916
FaqI	GGGAC	6 cut(s)	239, 718, 1003, 1847, 2044, 2767
FatI	CATG	9 cut(s)	252, 700, 818, 1080, 1290, 1494, 1659, 1915, 1956
FauI	CCCGC	2 cut(s)	121, 639
FauNDI	CATATG	3 cut(s)	1138, 1381, 2529
FbaI	TGATCA	1 cut(s)	1051
FblI	GTMKAC	3 cut(s)	575, 1725, 2148
Fnu4HI	GCNGC	5 cut(s)	117, 428, 1100, 1317, 2087
FokI	GGATG	9 cut(s)	234, 349, 359, 428, 875, 1557, 1856, 1901, 2927
FriOI	GRGCYC	1 cut(s)	513
Fsp4HI	GCNGC	5 cut(s)	117, 428, 1100, 1317, 2087
FspBI	CTAG	5 cut(s)	1197, 1425, 1875, 2943, 3045
GlaI	GCGC	1 cut(s)	1653
GluI	GCNGC	5 cut(s)	117, 428, 1100, 1317, 2087
GsaI	CCCAGC	1 cut(s)	1974
GsuI	CTGGAG	4 cut(s)	1845, 2187, 2232, 3025
HaeII	RGCGCY	1 cut(s)	1655
HaeIII	GGCC	7 cut(s)	251, 407, 430, 599, 1462, 1633, 2405
HapII	CCGG	8 cut(s)	106, 187, 212, 248, 431, 579, 1742, 2577
HhaI	GCGC	1 cut(s)	1654
Hin1I	GRCGYC	1 cut(s)	360
Hin1II	CATG	9 cut(s)	256, 704, 822, 1084, 1294, 1498, 1663, 1919, 1960
Hin6I	GCGC	1 cut(s)	1652
HinP1I	GCGC	1 cut(s)	1652
HincII	GTYRAC	1 cut(s)	2877
HindII	GTYRAC	1 cut(s)	2877
HindIII	AAGCTT	1 cut(s)	1183
HpaII	CCGG	8 cut(s)	106, 187, 212, 248, 431, 579, 1742, 2577
Hpy99I	CGWCG	7 cut(s)	152, 164, 268, 473, 3027, 3030, 3033
HpyCH4III	ACNGT	8 cut(s)	1049, 1106, 1112, 1730, 1847, 2513, 2768, 2845
HpyCH4IV	ACGT	4 cut(s)	23, 147, 360, 2188
HpyF10VI	GCNNNNNNNGC	6 cut(s)	495, 1182, 1191, 1660, 2926, 2969
HpyF3I	CTNAG	7 cut(s)	383, 519, 1598, 1718, 2133, 2409, 2430
HpySE526I	ACGT	4 cut(s)	23, 147, 360, 2188
Hsp92I	GRCGYC	1 cut(s)	360
Hsp92II	CATG	9 cut(s)	256, 704, 822, 1084, 1294, 1498, 1663, 1919, 1960
HspAI	GCGC	1 cut(s)	1652
KroI	GCCGGC	1 cut(s)	211
KroNI	GCCGGC	1 cut(s)	213
Ksp22I	TGATCA	1 cut(s)	1051
LguI	GCTCTTC	2 cut(s)	2025, 2812
LmnI	GCTCC	8 cut(s)	296, 473, 508, 548, 652, 763, 2254, 2863
Lsp1109I	GCAGC	3 cut(s)	128, 1303, 2073
MaeI	CTAG	5 cut(s)	1197, 1425, 1875, 2943, 3045
MaeII	ACGT	4 cut(s)	23, 147, 360, 2188
MbiI	CCGCTC	2 cut(s)	551, 655
MfeI	CAATTG	1 cut(s)	963
MflI	RGATCY	7 cut(s)	124, 524, 1157, 2047, 2425, 2593, 3041
MhlI	GDGCHC	2 cut(s)	513, 1684
MlsI	TGGCCA	1 cut(s)	599
MluNI	TGGCCA	1 cut(s)	599
MlyI	GAGTC	4 cut(s)	985, 1874, 2194, 2726
MmeI	TCCRAC	2 cut(s)	1244, 1826
Mox20I	TGGCCA	1 cut(s)	599
Mph1103I	ATGCAT	2 cut(s)	835, 2326
MroNI	GCCGGC	1 cut(s)	211
MroXI	GAANNNNTTC	1 cut(s)	440
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	9 cut(s)	648, 683, 1119, 1277, 1668, 1701, 1784, 1851, 2103
MslI	CAYNNNNRTG	5 cut(s)	449, 950, 1199, 1772, 2156
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	1 cut(s)	2699
MspI	CCGG	8 cut(s)	106, 187, 212, 248, 431, 579, 1742, 2577
MspR9I	CCNGG	8 cut(s)	188, 301, 432, 1743, 1863, 2384, 2802, 2823
MunI	CAATTG	1 cut(s)	963
Mva1269I	GAATGC	3 cut(s)	358, 833, 2866
MvaI	CCWGG	5 cut(s)	301, 1863, 2384, 2802, 2823
MwoI	GCNNNNNNNGC	6 cut(s)	495, 1182, 1191, 1660, 2926, 2969
NaeI	GCCGGC	1 cut(s)	213
NciI	CCSGG	3 cut(s)	188, 432, 1743
NcoI	CCATGG	1 cut(s)	252
NdeI	CATATG	3 cut(s)	1138, 1381, 2529
NgoMIV	GCCGGC	1 cut(s)	211
NlaIII	CATG	9 cut(s)	256, 704, 822, 1084, 1294, 1498, 1663, 1919, 1960
NmuCI	GTSAC	4 cut(s)	2206, 2434, 2729, 2945
NsiI	ATGCAT	2 cut(s)	835, 2326
NspI	RCATGY	2 cut(s)	1663, 1960
NspV	TTCGAA	1 cut(s)	640
OliI	CACNNNNGTG	3 cut(s)	1199, 1772, 2156
PaqCI	CACCTGC	1 cut(s)	1935
PciI	ACATGT	1 cut(s)	1956
PciSI	GCTCTTC	2 cut(s)	2025, 2812
PcsI	WCGNNNNNNNCGW	3 cut(s)	69, 156, 452
PctI	GAATGC	3 cut(s)	358, 833, 2866
PdiI	GCCGGC	1 cut(s)	213
PdmI	GAANNNNTTC	1 cut(s)	440
PfeI	GAWTC	8 cut(s)	156, 1376, 1591, 1753, 2169, 2278, 2447, 2504
PflMI	CCANNNNNTGG	2 cut(s)	986, 2210
PfoI	TCCNGGA	2 cut(s)	1741, 2382
PinAI	ACCGGT	2 cut(s)	578, 2576
PkrI	GCNGC	5 cut(s)	118, 429, 1101, 1318, 2088
PleI	GAGTC	4 cut(s)	985, 1874, 2194, 2726
PpsI	GAGTC	4 cut(s)	985, 1874, 2194, 2726
Ppu21I	YACGTR	1 cut(s)	2189
PscI	ACATGT	1 cut(s)	1956
PshAI	GACNNNNGTC	2 cut(s)	2204, 2523
PshBI	ATTAAT	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	513
Psp6I	CCWGG	5 cut(s)	299, 1861, 2382, 2800, 2821
PspFI	CCCAGC	1 cut(s)	1970
PspGI	CCWGG	5 cut(s)	299, 1861, 2382, 2800, 2821
PspPI	GGNCC	8 cut(s)	405, 887, 901, 977, 1331, 1461, 1834, 2403
PstNI	CAGNNNCTG	1 cut(s)	2210
PsuI	RGATCY	7 cut(s)	124, 524, 1157, 2047, 2425, 2593, 3041
RsaI	GTAC	9 cut(s)	167, 583, 867, 1087, 1171, 2063, 2367, 2418, 2469
RsaNI	GTAC	9 cut(s)	166, 582, 866, 1086, 1170, 2062, 2366, 2417, 2468
RseI	CAYNNNNRTG	5 cut(s)	449, 950, 1199, 1772, 2156
SacI	GAGCTC	1 cut(s)	513
SapI	GCTCTTC	2 cut(s)	2025, 2812
SaqAI	TTAA	9 cut(s)	648, 683, 1119, 1277, 1668, 1701, 1784, 1851, 2103
SatI	GCNGC	5 cut(s)	117, 428, 1100, 1317, 2087
Sau96I	GGNCC	8 cut(s)	405, 887, 901, 977, 1331, 1461, 1834, 2403
ScaI	AGTACT	2 cut(s)	1087, 2063
SchI	GAGTC	4 cut(s)	985, 1874, 2194, 2726
ScrFI	CCNGG	8 cut(s)	188, 301, 432, 1743, 1863, 2384, 2802, 2823
SduI	GDGCHC	2 cut(s)	513, 1684
SfcI	CTRYAG	2 cut(s)	1045, 2700
SfuI	TTCGAA	1 cut(s)	640
SgrAI	CRCCGGYG	1 cut(s)	2576
SinI	GGWCC	5 cut(s)	887, 901, 977, 1331, 1834
SmiMI	CAYNNNNRTG	5 cut(s)	449, 950, 1199, 1772, 2156
SmlI	CTYRAG	2 cut(s)	874, 2554
SmoI	CTYRAG	2 cut(s)	874, 2554
SpeI	ACTAGT	1 cut(s)	1196
SspI	AATATT	1 cut(s)	646
SspMI	CTAG	5 cut(s)	1197, 1425, 1875, 2943, 3045
SstI	GAGCTC	1 cut(s)	513
StyD4I	CCNGG	8 cut(s)	186, 299, 430, 1741, 1861, 2382, 2800, 2821
StyI	CCWWGG	2 cut(s)	252, 1634
TaaI	ACNGT	8 cut(s)	1049, 1106, 1112, 1730, 1847, 2513, 2768, 2845
TaiI	ACGT	4 cut(s)	26, 150, 363, 2191
TaqII	GACCGA	1 cut(s)	2605
TatI	WGTACW	8 cut(s)	165, 865, 1085, 1169, 2061, 2365, 2416, 2467
TauI	GCSGC	2 cut(s)	430, 1102
TfiI	GAWTC	8 cut(s)	156, 1376, 1591, 1753, 2169, 2278, 2447, 2504
Tru1I	TTAA	9 cut(s)	648, 683, 1119, 1277, 1668, 1701, 1784, 1851, 2103
Tru9I	TTAA	9 cut(s)	648, 683, 1119, 1277, 1668, 1701, 1784, 1851, 2103
TscAI	CASTG	5 cut(s)	483, 1054, 2215, 2886, 2954
TseFI	GTSAC	4 cut(s)	2206, 2434, 2729, 2945
TseI	GCWGC	3 cut(s)	116, 1316, 2086
Tsp45I	GTSAC	4 cut(s)	2206, 2434, 2729, 2945
TspDTI	ATGAA	5 cut(s)	37, 528, 1307, 1398, 1596
TspGWI	ACGGA	4 cut(s)	281, 486, 2241, 2348
TspRI	CASTG	5 cut(s)	483, 1054, 2215, 2886, 2954
Van91I	CCANNNNNTGG	2 cut(s)	986, 2210
VneI	GTGCAC	1 cut(s)	1680
VpaK11BI	GGWCC	5 cut(s)	887, 901, 977, 1331, 1834
VspI	ATTAAT	1 cut(s)	1701
XapI	RAATTY	3 cut(s)	1936, 2680, 2836
XceI	RCATGY	2 cut(s)	1663, 1960
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2878
XmiI	GTMKAC	3 cut(s)	575, 1725, 2148
XmnI	GAANNNNTTC	1 cut(s)	440
XspI	CTAG	5 cut(s)	1197, 1425, 1875, 2943, 3045
ZraI	GACGTC	1 cut(s)	361
ZrmI	AGTACT	2 cut(s)	1087, 2063
Zsp2I	ATGCAT	2 cut(s)	835, 2326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.